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Nanomaterials (Basel) ; 9(10)2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31640135


For the enhancement of the anticorrosion and antibacterial performance of the biomedical alloy Ti6Al4V, a novel Cu incorporated multilayer Ta2O5ceramic composite coating Cu-Ta2O5/Ta2O5/Ta2O5-TiO2/TiO2/Ti (coating codeCu-MTa2O5) was developed by radio frequency (RF) and direct current (DC) reactive magnetron sputtering. Meanwhile, to better display the multilayer Ta2O5 coating mentioned above, a monolayer Ta2O5 ceramic coating was deposited onto the surface of Ti6Al4V alloy as a reference. The surface morphology, microstructure, phase constituents, and elemental states of the coating were evaluated by atomic force microscopy, scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy, respectively. The adhesion strength, wettability, anticorrosion and antibacterial properties of the coating were examined by a scratch tester, contact angle measurement, electrochemical workstations, and plate counting method, respectively. The results showed that the deposited coatings were amorphous and hydrophobic. Cu doped into the Ta2O5 coating existed as CuO and Cu2O. A Ta2O5-TiO2/TiO2/Ti multi-interlayer massively enhanced the adhesion strength of the coating, which was 2.9 times stronger than that of the monolayer Ta2O5coating. The multilayer Cu-MTa2O5 coating revealed a higher corrosion potential and smaller corrosion current density as compared to the uncoated Ti6Al4V, indicating the better anticorrosion performance of Ti6Al4V. Moreover, a 99.8% antibacterial effect of Cu-MTa2O5 coated against Staphylococcus aureuswas obtained.

Medicine (Baltimore) ; 96(14): e6552, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28383426


BACKGROUND: Previous studies investigating the association between BRAF mutations and nonsmall cell lung cancer (NSCLC) remain controversial. To address the issue, we performed an updated meta-analysis of related articles. METHODS: We conducted a comprehensive literature search in the electronic databases including ISI Science Citation Index, EMBASE, PubMed, and CNKI (up to January 2016). The odds ratios (ORs) and 95% confidence interval (CI) were assessed based on random-effects or fixed-effects models according to the heterogeneity of eligible studies. RESULTS: A total of 16 studies enrolled 11,711 patients with NSCLC were involved in the meta-analysis. The overall BRAF mutation rate was 2.6% (303/11,711). There was a significant association between BRAF mutations and adenocarcinomas (ADCs) in NSCLC compared with non-ADCs (OR = 3.96, 95% CI = 2.13-7.34, P < 0.0001). No significant difference was observed in smoking and stage in patients with BRAF mutations. However, a significant difference of BRAF mutation rate was observed between women and men (OR = 0.72, 95% CI = 0.55-0.95, P = 0.02). In addition, the BRAF mutations were more frequent in women (OR = 0.45, 95% CI = 0.26-0.77, P = 0.004) and never smokers (OR = 0.12, 95% CI = 0.05-0.29, P < 0.00001). CONCLUSIONS: BRAF mutations in ADCS and female significantly increased the risk of NSCLC compared to non-ADCS and male, respectively. BRAFV mutation in NSCLC patients was significantly associated with female and nonsmokers.

Carcinoma Pulmonar de Células não Pequenas/genética , Neoplasias Pulmonares/genética , Proteínas Proto-Oncogênicas B-raf/genética , Humanos
Exp Biol Med (Maywood) ; 242(1): 45-52, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27480251


Increasing evidence suggests that miR-194 is down-regulated in esophageal squamous cell carcinoma tumor tissue. However, the role and underlying mechanism of miR-194 in esophageal squamous cell carcinoma have not been well defined. We used DIANA, TargetScan and miRanda to perform target prediction analysis and found KDM5B is a potential target of miR-194. Based on these findings, we speculated that miR-194 might play a role in esophageal squamous cell carcinoma development and progression by regulation the expression of KDM5B. We detected the expression of miR-194 and KDM5B by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot assays, respectively, and found down-regulation of miR-194 and up-regulation of KDM5B existed in esophageal squamous cell carcinoma cell lines. By detecting proliferation, invasion and apoptosis of TE6 and TE14 cells transfected with miR-194 mimics or mimic control, miR-194 was found to inhibit proliferation and invasion and promote apoptosis of esophageal squamous cell carcinoma cells. miR-194 was further verified to regulate proliferation, apoptosis and invasion of esophageal squamous cell carcinoma cells by directly targeting KDM5B. Furthermore, animal studies were performed and showed that overexpression of miR-194 inhibited the growth of esophageal squamous cell carcinoma tumors in vivo. These results confirmed our speculation that miR-194 targets KDM5B to inhibit esophageal squamous cell carcinoma development and progression. These findings offer new clues for esophageal squamous cell carcinoma development and progression and novel potential therapeutic targets for esophageal squamous cell carcinoma.

Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Neoplasias Esofágicas/genética , Neoplasias Esofágicas/patologia , Histona Desmetilases com o Domínio Jumonji/genética , MicroRNAs/genética , Proteínas Nucleares/genética , Proteínas Repressoras/genética , Regiões 3' não Traduzidas , Animais , Apoptose/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação para Baixo , Carcinoma de Células Escamosas do Esôfago , Regulação Neoplásica da Expressão Gênica , Humanos , Histona Desmetilases com o Domínio Jumonji/metabolismo , Masculino , Camundongos Endogâmicos BALB C , Proteínas Nucleares/metabolismo , Proteínas Repressoras/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto