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1.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 35(10): 1251-1258, 2021 Oct 15.
Artigo em Chinês | MEDLINE | ID: mdl-34651477

RESUMO

Objective: To explore the short-term effectiveness of domestic robot-assisted total knee arthroplasty (RATKA) conducted by a prospective randomized controlled trial. Methods: Patients who were scheduled for primary unilateral TKA between October 2020 and December 2020 were eligible in this randomized controlled trial. According to the random number table method, they were allocated to the traditional TKA group and the RATKA group [application of the Yuanhua robotic-assisted TKA (YUANHUA-TKA) system during operation]. A total of 63 patients met the selection criteria were enrolled in the study, of which 3 cases voluntarily withdrew from the trial. And finally 60 cases were enrolled for analysis; of which 28 cases were in the RATKA group and 32 cases were in the traditional TKA group. There was no significant difference in gender, age, body mass index, American Society of Anesthesiologists (ASA) classification, duration of osteoarthritis, surgical side, and preoperative knee visual analogue scale (VAS) resting and motion scores, joint range of motion (ROM), Knee Society Score (KSS), Western Ontario and McMaster University Osteoarthritis Index (WOMAC) pain, stiffness, and function scores, hip-knee-ankle angle (HKA) deviation ( P>0.05). The operation time and intraoperative blood loss of the two groups were recorded. Knee joint VAS resting and motion scores, ROM, KSS scores, and WOMAC pain, stiffness, and function scores were used to evaluate the knee joint function and pain. Gait analysis (flexion and extension angle) was conducted at 3 months after operation. The full length X-ray films of lower limbs and anteroposterior and lateral X-ray films of knee joint were taken. The HKA deviation, lateral tibia component (LTC), frontal femoral component (FFC), frontal tibia component (FTC), and lateral femoral component (LFC) measured on the X-ray films were used to evaluat the lower limb alignment and prosthesis position. Results: The operations of the two groups completed successfully; the incisions healed by first intention after operation, and no complications related to the operation occurred. The operation time of the RATKA group was significantly longer than that of the traditional TKA group ( t=12.253, P=0.001), and there was no significant difference in intraoperative blood loss between the two groups ( t=3.382, P=0.071). All patients were followed up 3 months. At 3 months after operation, the knee joint VAS resting and motion scores, ROM, KSS scores, and WOMAC pain, stiffness, and function scores improved significantly when compared with preoperatively in the two groups ( P<0.05); there was no significant difference of pre- and post-operative indicators between the two groups ( P>0.05). The gait analysis showed that the flexion and extension angle in the RATKA group was significantly bigger than that in the traditional TKA group ( t=9.469, P=0.003). X-ray films reexamination at 3 months after operation showed that the prostheses in the two groups were in good positions, and there was no adverse events such as prosthesis loosening or sinking. There were significant differences in the HKA deviation between pre- and post-operation in the two groups ( P<0.05), but the difference of pre- and post-operative HKA deviation between the two groups was not significant ( t=1.254, P=0.267). There was no significant difference in FFC, FTC, and LFC between the two groups ( P>0.05); the LTC was significantly smaller in the RATKA group than in the traditional TKA group ( t=17.819, P=0.000), which was closer to the ideal value. Conclusion: YUANHUA-TKA system can improve the accuracy of osteotomy and the prosthesis placement as well as the lower limb alignment. Its short-term effectiveness can be promised, but long-term effectiveness needs to be further studied.

2.
Sci Total Environ ; 804: 150125, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34520912

RESUMO

Phosphorus (P) from detergents contributes to water pollution and eutrophication. Understanding the impacts of detergent use on P inputs to surface waters and their main drivers is vital for supporting Sustainable Development Goals on clean water. This study aims to quantify past and future trends in P inputs to surface waters from detergent use in China. We modify the Model to Assess River Input of Nutrient to seAs (MARINA) model to assess the effects of past policies and explore options for the future on mitigating detergents P losses in China. The total consumption of detergents tripled from 2000 to 2018. However, P inputs to surface waters from detergent use decreased by 35% during these years. Although P losses vary across regions, most losses occurred in rural areas. Clearly, the P-free detergent policy which was initiated in the year 2000 has been effective. Without this policy, the detergent P losses would likely have increased fourfold during 2000-2018. In the future, detergent P inputs to surface waters in China may be further reduced to very low levels (95% reduction relative to 2018) by a combination of completely P-free detergents, an increasing urbanized population connected to sewage systems, and improving P removal in sewage treatment systems. Our results enhance the understanding of P pollution in surface waters from detergents and, illustrate the effectiveness of measures to control detergent P losses.

3.
Pain Manag Nurs ; 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34474997

RESUMO

PURPOSE: To examine the association of hypertension with knee pain severity in individuals with knee osteoarthritis (OA). DESIGN: Cross-sectional study of baseline data collected by the Osteoarthritis Initiative. METHODS: Participants with knee OA (N=1,363) were categorized into four groups based on blood pressure (BP): 1) systolic < 120 mm HG and diastolic < 80 mm Hg; 2) 120 ≤ systolic < 130 mm Hg and diastolic < 80 mm Hg; 3) 130 ≤ systolic < 140 mm Hg or 80 ≤ diastolic < 90 mm Hg; 4) systolic ≥ 140 mm Hg or diastolic ≥ 90 mm Hg. OA knee pain severity was measured by Pain subscale of Western Ontario and McMaster Universities Osteoarthritis Index in the past 48 hours, Pain subscale of Knee Injury and Osteoarthritis Outcome Score (KOOS) in the past 7 days, and numeric rating scale (NRS) in the past 30 days. Linear regression was used to examine the relationship between hypertension and knee pain severity. RESULTS: Compared with the normal BP group, individuals with stage 2 hypertension reported significantly higher OA knee pain severity by KOOS in the past 7 days (ß=-2.05 [95% CI -4.09, -0.01], p=0.049) and by NRS in the past 30 days (ß=0.31 [95% CI 0.01, 0.62], p=0.045) after adjustments for demographic and medical factors. CONCLUSIONS: Hypertension was associated with higher OA knee pain severity in individuals with knee OA. CLINICAL IMPLICATIONS: Nurses can recommend adjunctive non-pharmacological treatments and adherence strategies to help control hypertension, which may help decrease OA knee pain.

4.
Sensors (Basel) ; 21(18)2021 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-34577467

RESUMO

Different cultivars of pear trees are often planted in one orchard to enhance yield for its gametophytic self-incompatibility. Therefore, an accurate and robust modelling method is needed for the non-destructive determination of leaf nitrogen (N) concentration in pear orchards with mixed cultivars. This study proposes a new technique based on in-field visible-near infrared (VIS-NIR) spectroscopy and the Adaboost algorithm initiated with machine learning methods. The performance was evaluated by estimating leaf N concentration for a total of 1285 samples from different cultivars, growth regions, and tree ages and compared with traditional techniques, including vegetation indices, partial least squares regression, singular support vector regression (SVR) and neural networks (NN). The results demonstrated that the leaf reflectance responded to the leaf nitrogen concentration were more sensitive to the types of cultivars than to the different growing regions and tree ages. Moreover, the AdaBoost.RT-BP had the best accuracy in both the training (R2 = 0.96, root mean relative error (RMSE) = 1.03 g kg-1) and the test datasets (R2 = 0.91, RMSE = 1.29 g kg-1), and was the most robust in repeated experiments. This study provides a new insight for monitoring the status of pear trees by the in-field VIS-NIR spectroscopy for better N managements in heterogeneous pear orchards.


Assuntos
Pyrus , Análise dos Mínimos Quadrados , Aprendizado de Máquina , Nitrogênio , Espectroscopia de Luz Próxima ao Infravermelho
5.
Mol Med Rep ; 24(4)2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34435646

RESUMO

Chronic hepatitis B can lead to liver cirrhosis and primary hepatocellular carcinoma. The present study aimed to investigate whether C­X­C motif chemokine receptor 3 (CXCR3) regulates the genes in Toll­like receptors (TLRs)/myeloid differentiation primary response protein 88 (MyD88) signaling pathway in the development of hepatitis B into cirrhosis and liver cancer in vitro. A hepatitis B virus (HBV) overexpression lentivirus was constructed and infected into a LX­2 cell line to obtain stable HBV­overexpressing cells (named HBV­LX­2 cells). The CXCR3 gene was knocked down using small interfering RNA in HBV­LX­2 cells. Cell Counting Kit­8 assays, cell scratch tests and flow cytometry were used to detect cell proliferation, migration and apoptosis, respectively. The levels of IL­1ß and IL­6 in serum samples of patients with liver cancer were measured via ELISA, and the collagen content in liver cancer tissues was detected using Masson staining. Western blotting was used to detect the expression levels of proteins in the TLRs/MyD88 signaling pathway. Excessive fibrosis was identified in the liver cancer tissues, and the serum levels of IL­6 and IL­1ß were abnormally increased in patients with liver cancer. It was found that interfering with CXCR3 inhibited cell proliferation and migration, as well as promoted the apoptosis of HBV­LX­2 cells. Moreover, interfering with CXCR3 inhibited the expression levels of collagen type I α 1 chain and the proteins in the TLRs/MyD88 pathway. In conclusion, CXCR3 knockdown could inhibit the expression levels of proteins in the TLR4/MyD88 signaling pathway, decrease cell proliferation and migration, and promote cell apoptosis, thus inhibiting the development of liver cirrhosis to liver cancer.

6.
Trials ; 22(1): 545, 2021 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-34407832

RESUMO

BACKGROUND: Proton pump inhibitor (PPI) is effective for the treatment of nonerosive gastroesophageal reflux (NERD), but long-term use of PPI is prone to have complications and recurrence after withdrawal. Traditional Chinese medicine (TCM) can relieve the symptoms of reflux and improve the quality of life. The purpose of this study is to evaluate the safety and efficacy of Hewei Jiangni recipe (HWJNR) in the treatment of NERD with cold-heat complex syndrome, and clarify the mechanism of HWJNR on NERD based on the correlation analysis of intestinal flora and metabolites. METHODS: This is a single-center, randomized controlled, double-blind, placebo-controlled clinical trial in which 72 eligible participants with NERD and TCM syndrome of intermingled heat and cold will be randomly allocated in the ratio of 1:1 to two groups: TCM group and western medicine group. The TCM group will receive HWJNR with omeprazole enteric-coated tablets placebo, while the western medicine group will receive omeprazole enteric-coated tablets with HWJNR placebo. Each group will be treated for 8 weeks. The primary outcome is the score of gastroesophageal reflux disease (GERD) health-related quality of life questionnaire (GERD-Q). Secondary outcomes include SF-36 quality of life scale (SF-36), patient-reported outcomes (PRO) self-rating scale score, syndrome score of TCM, and adverse events. Mechanistic outcome is the correlation analysis of intestinal flora and metabolites from healthy individuals and NERD participants before and after the treatment respectively. DISCUSSION: The goal of this trial is to investigate the efficacy and safety of HWJNR in the treatment of NERD with cold-heat complex syndrome, and to study the composition structure and metabolite expression profile of intestinal flora in patients with NERD through 16SrRNA sequencing and metabolomic correlation analysis of fecal flora, which makes us identify the dominant links of treatment and reveal the potential mechanism of HWJNR. ChiCTR2000041225 . Registered on 22 December 2020.


Assuntos
Medicamentos de Ervas Chinesas , Qualidade de Vida , China , Método Duplo-Cego , Medicamentos de Ervas Chinesas/efeitos adversos , Temperatura Alta , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
7.
Artigo em Inglês | MEDLINE | ID: mdl-34374557

RESUMO

Ralstonia solanacearum RSc0454 is predicated as a FAD-linked oxidase based on protein homologies, while containing distinct domains of LDH and SDH. Current study demonstrates RSc0454 exhibits LDH activity and is essential for pathogenicity. Here, we characterized involvement of RSc0454 on bacterial growth and expression of the T3SS in R. solanacearum. RSc0454 mutant grew normally in rich medium but grew faintly in host plants, and failed to grow in minimal medium. Supplementary succinate, but not lactate, substantially restored some phenotypes of RSc0454 mutants, including faint growth in plants, diminished growth in minimal medium, and lost pathogenicity. The T3SS Expression is directly controlled by a master regulator HrpB, and HrpG and PrhG positively regulate hrpB expression in parallel ways. Deletion of RSc0454 substantially reduced expression levels of hrpB and T3SS both in vitro and in planta. Moreover, RSc0454 is revealed to be required for the T3SS expression via HrpG and PrhG, but through novel pathway, and impaired expression of these genes was not due to growth deficiency of RSc0454 mutants. RSc0454 is suggested to be important for redox balance inside cells and supplementary NADH partially restored diminished growth of RSc0454 mutant in minimal medium at presence of succinate at some moderate concentrations, indicating that unbalanced redox in RSc0454 mutant might be responsible for its no growth in minimal medium. All taken together, these results provide novel insights into understanding of various biological functions of this FAD-linked oxidase RSc0454 and involvement of the redox balance on expression of the T3SS in R. solanacearum.

8.
J Colloid Interface Sci ; 603: 252-258, 2021 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-34186403

RESUMO

Rechargeable zinc-air batteries (ZABs) is primarily driven by the couple of oxygen evolution reaction (OER) and oxygen reduction reaction (ORR). Currently,it is still challenging to develop cost-effective, highly efficient, and robust bifunctional catalysts for ZABs. Herein, a novel silver decorated cobalt carbonate (Ag@CoCO3) hybrid catalyst is proposed as the potential bifunctional catalyst to drive OER and ORR for ZABs. Engineering Ag nanoparticles onto the surface of CoCO3 microsphere not only facilitates the charge transfer, but also modulates the electronic structure, which are beneficial to intrinsic bifunctional activity. As a result, this Ag@CoCO3 catalyst yields a substantially enhanced bifunctionality compared to the pristine CoCO3 catalyst. Moreover, the homemade Ag@CoCO3 based ZABs provides a high peak power density of 146 mW cm-2, superior to 107 mW cm-2 for CoCO3 based ZABs and 111 mW cm-2 for commercial Pt/C-IrO2 based ZABs.

9.
Sci Total Environ ; 786: 147361, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-33971610

RESUMO

Animal manure fertilization facilitates the proliferation and dissemination of antibiotic resistance genes (ARGs) in soil, posing high risks to humans and ecosystem health. Although studies suggest that soil types could shape the ARG profiles in greenhouse soil, there is still a lack of comparative studies on the fate of ARGs in different types of manured soils under field trials. Thus, a metagenomic approach was used to decipher the fate of ARGs in 12-year long-term fertilized (inorganic fertilizer, compost manure and a mix of them) acidic, near-neutral and alkaline soils. A total of 408 unique ARG subtypes with multidrug, glycopeptide, beta-lactam and aminoglycoside resistance genes were identified as the most universal ARG types in all soil samples. Genes conferred to beta-lactam was the predominant ARG type in all the manure-amended soils. Genomic and statistical analyses showed that manure application caused the enrichment of 98 and 91 ARG subtypes in acidic and near-neutral soils, respectively, and 8 ARG subtypes in alkaline soil. The abundances of Proteobacteria (acidic and near-neutral soils) and Actinobacteria (alkaline soil), which are the potential hosts of ARGs, were clearly increased in manured soils. Random forest modelling and Pearson correlation analysis revealed that the soil properties (pH and bio-available Zn) and mobile genetic elements had considerable impacts on the transmission of ARGs. A structural equation model further indicated that soil types shaped the ARG profiles by significantly (P < 0.01) influencing the soil properties, bacterial abundance and bacterial diversity, where bacterial abundance was the major factor influencing the ARG profiles. This study systematically explored the mechanisms shaping the ARG profiles of long-term manured soils, and this information could support strategies to manage the dissemination of ARGs in different soil types.


Assuntos
Esterco , Solo , Animais , Antibacterianos , Ecossistema , Genes Bacterianos , Humanos , Microbiologia do Solo
10.
Chin Med J (Engl) ; 134(7): 829-839, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33538509

RESUMO

BACKGROUND: MicroRNAs are closely associated with the progression and outcomes of multiple human diseases, including sepsis. In this study, we examined the role of miR-23a in septic injury. METHODS: Lipopolysaccharide (LPS) was used to induce sepsis in a rat model and H9C2 and HK-2 cells. miR-23a expression was evaluated in rat myocardial and kidney tissues, as well as H9C2 and HK-2 cells. A miR-23a mimic was introduced into cells to identify the role of miR-23a in cell viability, apoptosis, and the secretion of inflammatory cytokines. Furthermore, the effect of Rho-associated kinase 1 (ROCK1), a miR-23a target, on cell damage was evaluated, and molecules involved in the underlying mechanism were identified. RESULTS: In the rat model, miR-23a was poorly expressed in myocardial (sham vs. sepsis 1.00 ±â€Š0.06 vs. 0.27 ±â€Š0.03, P < 0.01) and kidney tissues (sham vs. sepsis 0.27 ±â€Š0.03 vs. 1.00 ±â€Š0.06, P < 0.01). Artificial overexpression of miR-23a resulted in increased proliferative activity (DNA replication rate: Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 34.13 ±â€Š3.12 vs. 12.94 ±â€Š1.21 vs. 13.31 ±â€Š1.43 vs. 22.94 ±â€Š2.26, P < 0.05; HK-2 cells: 15.17 ±â€Š1.43 vs. 34.52 ±â€Š3.46 vs. 35.19 ±â€Š3.12 vs. 19.87 ±â€Š1.52, P < 0.05), decreased cell apoptosis (Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 11.39 ±â€Š1.04 vs. 32.57 ±â€Š2.29 vs. 33.08 ±â€Š3.12 vs. 21.63 ±â€Š2.35, P < 0.05; HK-2 cells: 15.17 ±â€Š1.43 vs. 34.52 ±â€Š3.46 vs. 35.19 ±â€Š3.12 vs. 19.87 ±â€Š1.52, P < 0.05), and decreased production of inflammatory cytokines, including interleukin-6 (Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 59.61 ±â€Š5.14 vs. 113.54 ±â€Š12.30 vs. 116.51 ±â€Š10.69 vs. 87.69 ±â€Š2.97 ng/mL; P < 0.05, F = 12.67, HK-2 cells: 68.12 ±â€Š6.44 vs. 139.65 ±â€Š16.62 vs. 143.51 ±â€Š13.64 vs. 100.82 ±â€Š9.74 ng/mL, P < 0.05, F = 9.83) and tumor necrosis factor-α (Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 103.20 ±â€Š10.31 vs. 169.67 ±â€Š18.84 vs. 173.61 ±â€Š15.91 vs. 133.36 ±â€Š12.32 ng/mL, P < 0.05, F = 12.67, HK-2 cells: 132.51 ±â€Š13.37 vs. 187.47 ±â€Š16.74 vs. 143.51 ±â€Š13.64 vs. 155.79 ±â€Š15.31 ng/mL, P < 0.05, F = 9.83) in cells. However, ROCK1 was identified as a miR-23a target, and further up-regulation of ROCK1 mitigated the protective function of miR-23a in LPS-treated H9C2 and HK-2 cells. Moreover, ROCK1 suppressed sirtuin-1 (SIRT1) expression to promote the phosphorylation of nuclear factor-kappa B (NF-κB) p65, indicating the possible involvement of this signaling pathway in miR-23a-mediated events. CONCLUSION: Our results indicate that miR-23a could suppress LPS-induced cell damage and inflammatory cytokine secretion by binding to ROCK1, mediated through the potential participation of the SIRT1/NF-κB signaling pathway.


Assuntos
Lipopolissacarídeos , MicroRNAs , Animais , Apoptose/genética , Linhagem Celular , Citocinas , Inflamação/genética , MicroRNAs/genética , NF-kappa B , Ratos , Sirtuína 1 , Quinases Associadas a rho/genética
11.
Geriatr Nurs ; 42(2): 517-523, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33039202

RESUMO

The purpose of this study was to describe older adults' social network and support during a physical activity intervention and its association with physical activity. Mixed methods were used for this secondary analysis of existing data from a physical activity intervention. Seventy-three participants who completed a session by telephone on using support comprised the sample. Participants reported on average approximately eight individuals in their social network. Half reported support for physical activity from a spouse/partner, adult child, or friend. Those who perceived support for self-reported physical activity tended to have greater improvements on average from baseline for mean daily minutes of physical activity than those with no perceived support. Four-fifths developed a plan for a spouse/partner, friend, adult child, or sibling to provide physical activity support. Participants reported that the session was helpful and increased their confidence in using support; over one-quarter intended to use support after the intervention ended.

12.
Sci Total Environ ; 752: 141795, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32892043

RESUMO

Sugarcane is the second largest bioenergy crop in the world and it accounts for 80% of global sugar production. Grown mostly in wet and warm tropics with relatively high nitrogen (N) fertiliser input and crop residue retention, sugarcane production is a significant source of nitrous oxide (N2O) emission. Yet, a global evaluation of research on N2O emission from sugarcane crop is lacking. Here, we conducted a meta-analysis using data from 141 measurements compiled from 15 sugarcane field studies reported from different countries to i) quantify N2O emissions and emission factors (EFs) globally, and for tropics and sub-tropics, and ii) identify the key factors that promote N2O emission. Our analysis shows that the global mean total N2O emission from sugarcane production reached 2.26 (CI: 1.93-2.62) kg N2O-N ha-1 yr-1 with an estimated EF of 1.21% (CI: 0.971-1.46%). N2O emissions increased exponentially with increase in N fertiliser rate, questioning the adequacy of Intergovernmental Panel on Climate Change (IPCC) default EF value (1%) for sugarcane N2O emission estimation. Mean total N2O emissions and EFs in tropics and sub-tropics did not vary significantly. Supplementing synthetic N fertiliser (SN) with organic amendments (OA) significantly increased mean N2O emission (~1.4-fold) and EF (~2.5-fold) compared to SN. A remarkable reduction in N2O emission (38.6%) and EF (61.5%) was evident when enhanced efficiency fertilisers (EEF) replaced SN. In contrast, crop residue removal had little impact on N2O emission and EF, but both parameters showed an upward trend with irrigation and increased rainfall. Soil carbon content and pH were emerged as key regulators of sugarcane N2O emission and EF. It is concluded that global sugarcane N2O emission could be significant and that there is considerable prospect for mitigating the emission through innovative nutrient formulations and precision agriculture that help meet crop nutrient demand without compromising environmental imperatives.


Assuntos
Óxido Nitroso , Saccharum , Agricultura , Fertilizantes/análise , Nitrogênio , Óxido Nitroso/análise , Solo
13.
PeerJ ; 8: e9928, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32999767

RESUMO

Acute lung injury (ALI) is associated with histopathological diffuse alveolar damage. The potential role of mesenchymal stem cells (MSCs) in the treatment of various clinical disorders have been widely documented, such as those for ALI. Recent evidence has demonstrated that exosomes from endothelial progenitor cells can improve outcomes of the lipopolysaccharide (LPS)-induced ALI. However, there has been no research on the potential role of MSC-exosomes in the treatment of sepsis-induced ALI, which is worth further exploration. Thus, the objective of our study was to identify whether the MSC-exosomes could reverse ALI. The ALI model induced by LPS was established in this study. MTT assay was performed to test cell proliferation. Expression of inflammatory factors (TNF-α, IL-6, and IL-10) in the LPS-treated type II alveolar epithelial cells (AECs) (MLE-12) was detected by ELISA. After co-culture of MSC-exosomes with LPS-treated MLE-12 cells, we found that the cell proliferation of MLE-12 cells gradually increased. Furthermore, we selected five of the Nrf-2/ARE- and NF-κB signaling pathway-related genes to explore if MSC-exosomes could reverse LPS-induced ALI through Nrf-2/ARE and NF-κB signaling pathways. QRT-PCR and western blot experiment results showed that the expression of these five genes were significantly regulated after stimulation with high-concentration LPS and exosome intervention. Taken together, these findings highlighted the fact that MSC-exosomes could reverse ALI through the Nrf-2/ARE and NF-κB signaling pathways. The MSC-exosome may be the potential future therapeutic strategy for the treatment of ALI.

14.
J Gerontol Nurs ; 46(11): 28-36, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33095890

RESUMO

The current study examined the prevalence of insomnia and its relationship with daily function in older adults with asthma. Data on 278 older adults with asthma (aged ≥60 years) from the 2005-2008 National Health and Nutrition Examination Survey were analyzed. Insomnia was present in 40% of the study sample and was associated with a greater number of activities of daily living (ADL) and instrumental ADL (IADL) limitations. In multivariate analyses accounting for covariates including depressive symptoms, insomnia was not associated with having at least two ADL/IADL limitations. However, those with co-occurring insomnia and depressive symptoms were more likely to have at least two ADL/IADL limitations compared to those with either condition singly. Insomnia in the presence of depressive symptoms may impede one's ability to perform ADL/IADL. Current findings suggest that assessment and treatment of insomnia and depression in older adults with asthma may be important for maintaining active, independent living. [Journal of Gerontological Nursing, 46(11), 28-36.].

15.
BMC Cardiovasc Disord ; 20(1): 404, 2020 09 10.
Artigo em Inglês | MEDLINE | ID: mdl-32912159

RESUMO

BACKGROUND: N-acetylneuraminic acid (Neu5Ac) is a functional metabolite involved in coronary artery disease (CAD). We aimed to evaluate the relationship between serum Neu5Ac and the risk and prognosis of acute coronary syndrome (ACS) in a real-world prospective study. METHODS: Patients with suspected ACS who underwent coronary angiography were included. Serum Neu5Ac was measured at admission. Coronary lesion severity was evaluated by Gensini Score. GRACE risk stratification was performed at admission. Major adverse cardiac events (MACEs) were recorded during follow-up. RESULTS: A total of 766 patients, including 537 with unstable angina (UAP), 100 with myocardial infarction (MI), and 129 without CAD were included. The circulating Neu5Ac level was significantly higher in patients with MI (median [1QR]: 297[220, 374] ng/ml) than in those with UAP (227 [114, 312] ng/ml) or without CAD (207 [114, 276] ng/ml; both p < 0.001). Serum level of Neu5Ac was positively correlated with age, hypertension, serum uric acid, creatinine, MB isoform of creatine kinase (CK-MB), and Gensini score (all p < 0.05). Receiver operating characteristic curve analysis showed that a higher serum Neu5Ac was potentially associated with MI and high-risk GRACE stratification in ACS patients. Logistic analysis identified only elevated serum Neu5Ac as an independent predictor of MACEs in these patients (odds ratio [OR]: 1.003, 95% confidence interval [CI]: 1.002-1.005, p < 0.001). CONCLUSIONS: Serum Neu5Ac is associated with myocardial injury, GRACE risk category, and prognosis in ACS patients.


Assuntos
Síndrome Coronariana Aguda/sangue , Ácido N-Acetilneuramínico/sangue , Síndrome Coronariana Aguda/diagnóstico por imagem , Idoso , Biomarcadores/sangue , Angiografia Coronária , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Admissão do Paciente , Prognóstico , Estudos Prospectivos , Medição de Risco , Fatores de Risco , Fatores de Tempo , Regulação para Cima
16.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(9): 1253-1258, 2020 Sep 30.
Artigo em Chinês | MEDLINE | ID: mdl-32990230

RESUMO

OBJECTIVE: To explore the correlation of plasma N-acetyl-neuraminic acid level with Thrombolysis In Myocardial Infarction (TIMI) risk score and clinical outcomes of patients with acute coronary syndrome (ACS). METHODS: We consecutively enrolled 708 consecutive patients (401 male and 307 female, mean age 63.6±10.6 years) undergoing coronary angiography in our hospital between October, 2018 and July, 2019, including 597 patients with ACS and 111 without ACS (control group). The patients with ACS group were divided into high (n=104), moderate (n=425) and low (n=68) risk groups according to their TIMI risk scores. All the participants were examined for plasma Neu5Ac level using liquid chromatography-tandem mass spectrometry and underwent coronary angiography with their Gensini scores calculated. The patients with ACS were followed up after discharge for a mean of 15 months for the occurrence of major adverse cardiac events (Mace). Binary logistic regression analysis was performed to identify the risk factors of Mace in these patients. RESULTS: Plasma Neu5Ac levels were significantly higher in ACS group than in the control group (P < 0.05). ROC curve analysis showed that plasma Neu5Ac level could assist in the diagnosis of ACS (0.648 [0.597-0.699]) with a sensitivity of 39.2% and a specificity of 86.5% at the cutoff value of 288.50 ng/mL. In the ACS patients, plasma Neu5Ac level was significantly higher in the high-risk group than in the moderate-risk and low-risk groups (P < 0.05) and could assist in the diagnosis of a high risk (0.645 [0.588-0.703]) with a sensitivity of 42.3% and a specificity of 80.1% at the cutoff value of 327.50 ng/ mL. Plasma Neu5Ac was positively correlated with age, serum uric acid, creatinine, lipoprotein a, Ddimer, C-reactive protein, MB isoform of creatine kinase and Gensini score and negatively correlated with high-density lipoprotein level. During the followup, 80 ACS patients experienced Mace, who had significantly higher plasma Neu5Ac level than those without Mace (n=517). Logistic regression analysis showed that plasma Neu5Ac level and a history of previous stroke were independent risk factors for the occurrence of Mace. CONCLUSIONS: Plasma Neu5Ac level can provide assistance in the diagnosis and risk stratification of ACS and is an independent risk factor for prognosis of ACS patients.


Assuntos
Síndrome Coronariana Aguda , Infarto do Miocárdio , Idoso , Angiografia Coronária , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição de Risco , Ácido Úrico
17.
Environ Sci Technol ; 54(19): 11929-11939, 2020 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-32856903

RESUMO

Agriculture contributes considerably to nitrogen (N) inputs to the world's rivers. In this study, we aim to improve our understanding of the contribution of different crops to N inputs to rivers. To this end, we developed a new model system by linking the MARINA 2.0 (Model to Assess River Input of Nutrient to seAs) and WOFOST (WOrld FOod STudy) models. We applied this linked model system to the Yangtze as an illustrative example. The N inputs to crops in the Yangtze River basin showed large spatial variability. Our results indicate that approximately 6,000 Gg of N entered all rivers of the Yangtze basin from crop production as dissolved inorganic N (DIN) in 2012. Half of this amount is from the production of single rice, wheat, and vegetables, where synthetic fertilizers were largely applied. In general, animal manure contributes 12% to total DIN inputs to rivers. Three-quarters of manure-related DIN in rivers are from vegetable, fruit, and potato production. The contributions of crops to river pollution differ among sub-basins. For example, potato is an important source of DIN in rivers of some upstream sub-basins. Our results may help to prioritize the dominant crop sources for management to mitigate N pollution in the future.


Assuntos
Rios , Poluentes Químicos da Água , Agricultura , China , Monitoramento Ambiental , Fertilizantes , Nitrogênio/análise , Oceanos e Mares , Poluentes Químicos da Água/análise
18.
J Environ Manage ; 270: 110888, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32721326

RESUMO

Nitrogen (N) fertilizer-induced soil acidification in Chinese croplands is well-known, but insight in the impacts of different N fertilizer management approaches (fertilizer type and rate) on soil acidification rates is very limited. Here, we conducted a field experiment on a moderate acid soil to quantify soil acidification rates in response to N fertilization by different fertilizer types and N rates through monitoring the fate of elements (mainly nutrients) related to H+ production and consumption. Two N fertilizer types (urea and NH4Cl) and three N rates (control, optimized and conventional, 0/120/240 kg N ha-1 for wheat, 0/160/320 kg N ha-1 for maize) were included. Nitrogen addition led to an average H+ production of 4.0, 8.7, 11.4, 29.7 and 52.6 keq ha-1 yr-1, respectively, for the control, optimized urea, conventional urea, optimized NH4Cl and conventional NH4Cl plots. This was accompanied with a decline in soil base saturation of 1-10% and in soil pH of 0.1-0.7 units in the topsoil (0-20 cm). Removal of base cations by crop harvesting and N transformations contributed ~70% and ~20% to the H+ production in the urea treated plots, being ~20% and ~75% in the NH4Cl treated plots, respectively. The large NH4+ input via fertilization in the NH4Cl treated plots strongly enhanced the H+ production induced by N transformations. The low contribution of N transformations to the H+ production in the urea treated plots was due to the limited NO3- leaching, induced by the high N losses to air caused by denitrification. Increased N addition by urea, however, strongly increased H+ production by enhanced plant uptake of base cations, mainly due to a large potassium uptake in straw. Our results highlight the important role of optimizing fertilizer form and N rate as well as straw return to the field in alleviating soil acidification.


Assuntos
Fertilizantes , Triticum , Agricultura , China , Concentração de Íons de Hidrogênio , Nitrogênio , Solo , Zea mays
19.
Gen Physiol Biophys ; 39(3): 219-228, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32525815

RESUMO

Alisol B 23-acetate (AB23A) is a natural triterpenoid isolated from Chinese herbal medicine and has a variety of biological functions, especially anti-cancer effects. However, the effects and mechanisms of AB23A in hepatocellular carcinoma (HCC) remain unclear. Cell viability, invasion and migration were measured by MTT, Transwell and wound healing assays, respectively. To detect cell cycle and apoptosis, a flow cytometry assay was used. Tumor xenograft experiment was performed to measure tumor growth. The enzymatic assay was used to determine the activity of matrix metalloproteinase (MMP)-2 and -9. Furthermore, the mRNA and protein levels of Bcl-2, Bax, Caspase-3/-9, MMP-2/-9 and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) were detected by RT-PCR and Western blotting assays. AB23A suppressed cell viability in a concentration-dependent manner, blocked cell cycle, and induced apoptosis via up-regulating Bax, Caspase-3 and Caspase-9, and down-regulating Bcl-2 in HCC cells both in vitro and in vivo. In addition, AB23A inhibited cell invasion and migration through down-regulating MMP-2/-9 activities. The effects of AB23A might be associated with the PI3K/Akt signaling pathway in HCC cells. Taken together, the present data demonstrated that AB23A might play a role in suppressing the progression of HCC, revealing the value of AB23A for hepatocellular carcinoma treatment in clinic.


Assuntos
Apoptose/efeitos dos fármacos , Carcinoma Hepatocelular/patologia , Colestenonas/farmacologia , Neoplasias Hepáticas/patologia , Animais , Proteínas Reguladoras de Apoptose , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Humanos , Fosfatidilinositol 3-Quinases , Transdução de Sinais , Ensaios Antitumorais Modelo de Xenoenxerto
20.
Oncol Lett ; 20(1): 308-316, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32565957

RESUMO

Immune/stromal-associated genes may be promising biomarkers for cancer diagnosis and the determination of clinical cancer treatment options. The aim of the present study was to identify prognostic stromal/immune-associated genes in renal cell carcinoma (RCC). RCC gene expression data (885 cases) were obtained from The Cancer Genome Atlas database. Immune/stromal scores were calculated by using the ESTIMATE package in R. Immune/stromal scores were significantly associated with Tumor-Node-Metastasis stage, clinical stage and overall survival rate (P<0.05). There were 419 differentially expressed genes (DEGs) based on immune scores and 738 DEGs based on stromal scores. Among these DEGs, 406 DEGs based on stromal scores and 252 DEGs based on immune scores were significantly associated with overall survival rate (P<0.05). The biological functions of these DEGs were primarily enriched in the 'immune response' and 'regulation of cell migration and proliferation'. These DEGs were observed in a protein-protein interaction network. A LASSO Cox regression model was used to build a prognostic 6 gene-based classifier, including the IL21R, ATP6V1C2, GBP1, P2RY10, GBP4 and TNNC2 genes [area under the curve (AUC) =0.776]. The predictive model which combined this classifier with clinical prognostic factors had a high accuracy in predicting patient survival in RCC (combined AUC =0.899). Taken together, these results demonstrated that there are significant associations between immune/stromal scores and clinicopathological staging. A set of tumor microenvironment-associated genes that have powerful prognostic value in patients with RCC were identified in the present study.

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