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1.
Food Chem ; 398: 133911, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35969989

RESUMO

A superior sectioning sample preparation protocol is the basic guarantee for maintaining data reliability in MALDI-TOF-MSI analysis. Despite significant advances in sample preparation, visualization of lipids in hard and dry cereals remains difficult due to their inherent physicochemical properties. Thus, a cryosections preparation method was designed via poly-l-lysine soaking, conductive tape adhesion and embedding medium fixation, which preserved the spatial integrity of lipids in cereals without causing analyte delocalization and obvious background interference. Higher signal intensity and clearer imaging of lipids in rice, wheat and barley (Qingke) were obtained using the established sample preparation method. Moreover, the spatial distribution of lipids was visualized in rice with different storage years, which found that the phosphatidylinositols (PIs) and phosphatidylglycerols (PGs) could be used for evaluation of rice aging degree. Our study provided molecular level guidance for further discussion of rice aging mechanism, rice quality evaluation and safety monitoring.


Assuntos
Grão Comestível , Lipídeos , Reprodutibilidade dos Testes , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
2.
Front Microbiol ; 13: 840562, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35369425

RESUMO

Antibiotic resistance is one of the most important environmental challenges. Microalgae has been considered as a promising green media for environmental purification. In this work, sulfadimethoxine (SDM) biodegradation potential of Chlorella sp. L38 and Phaeodactylum tricornutum MASCC-0025 is investigated. Experimental results indicated that the tested freshwater and marine microalgae strains presented stress response to SDM addition. For Chlorella sp. L38, it has a good adaptability to SDM condition via antioxidant enzyme secretion (SOD, MDA, and CAT up to 23.27 U/mg, 21.99 µmol/g, and 0.31 nmol/min/mg) with removal rate around 88%. P. tricornutum MASCC-0025 exhibited 100% removal of 0.5 mg/L SDM. With increasing salinity (adding a certain amount of NaCl) of cultivation media, the removal rate of SDM by microalgae increased. Although its adaptive process was slower than Chlorella sp. L38, the salinity advantage would facilitate enzyme accumulation. It indicated that microalgae could be used to remove SDM from freshwater and marine environment via suitable microalgae strain screening.

3.
Anal Cell Pathol (Amst) ; 2022: 9303081, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36090016

RESUMO

Background: GPNMB is a newly discovered tumour-promoting factor that may promote tumour cell progression by activating the PI3K/AKT pathway by EGFR. However, there are insufficient studies about GPNMB in ESCC. This study investigated the relationship between GPNMB and EGFR/PI3K pathway genes in ESCC. Methods: The expression levels of GPNMB, EGFR, p-PI3K, and Ki-67 were examined using immunohistochemistry. Statistical analysis was done by SPSS 22.0 and R. Results: GPNMB mRNA expression is higher in ESCC compared with paracancerous tissues. The expression of EGFR, PIK3CA, PIK3CB, and AKT1 was increased in GPNMB upregulated samples. GPNMB expression was positively correlated with EGFR, p-PI3K, and Ki-67 expression. GPNMB was expressed higher in the AJCC III stage, lymph node metastasis, and moderately poorly differentiated patients. EGFR was higher expressed in patients with vascular invasion; p-PI3K expression in Kazak was higher than that in Han; Ki-67 expression was higher in tumour size ≥ 3 cm. Patients with high expression of GPNMB, p-PI3K, and Ki-67 had worse OS. p-PI3K, Ki-67, nerve invasion, and lymphatic metastasis were independent risk factors, and postoperative adjuvant therapy was a protective factor in ESCC. Conclusion: As a tumour-promoting factor, GPNMB is expected to be a potential target for ESCC.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Esofágicas , Carcinoma de Células Escamosas do Esôfago , Carcinoma de Células Escamosas/metabolismo , Classe I de Fosfatidilinositol 3-Quinases , Receptores ErbB/genética , Receptores ErbB/metabolismo , Neoplasias Esofágicas/patologia , Humanos , Antígeno Ki-67 , Metástase Linfática , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Prognóstico
4.
Front Oncol ; 12: 897596, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091102

RESUMO

Objectives: A radiomics-based explainable eXtreme Gradient Boosting (XGBoost) model was developed to predict central cervical lymph node metastasis (CCLNM) in patients with papillary thyroid carcinoma (PTC), including positive and negative effects. Methods: A total of 587 PTC patients admitted at Binzhou Medical University Hospital from 2017 to 2021 were analyzed retrospectively. The patients were randomized into the training and test cohorts with an 8:2 ratio. Radiomics features were extracted from ultrasound images of the primary PTC lesions. The minimum redundancy maximum relevance algorithm and the least absolute shrinkage and selection operator regression were used to select CCLNM positively-related features and radiomics scores were constructed. Clinical features, ultrasound features, and radiomics score were screened out by the Boruta algorithm, and the XGBoost model was constructed from these characteristics. SHapley Additive exPlanations (SHAP) was used for individualized and visualized interpretation. SHAP addressed the cognitive opacity of machine learning models. Results: Eleven radiomics features were used to calculate the radiomics score. Five critical elements were used to build the XGBoost model: capsular invasion, radiomics score, diameter, age, and calcification. The area under the curve was 91.53% and 90.88% in the training and test cohorts, respectively. SHAP plots showed the influence of each parameter on the XGBoost model, including positive (i.e., capsular invasion, radiomics score, diameter, and calcification) and negative (i.e., age) impacts. The XGBoost model outperformed the radiologist, increasing the AUC by 44%. Conclusions: The radiomics-based XGBoost model predicted CCLNM in PTC patients. Visual interpretation using SHAP made the model an effective tool for preoperative guidance of clinical procedures, including positive and negative impacts.

5.
Int J Ophthalmol ; 15(9): 1476-1482, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36124192

RESUMO

AIM: To compare the ciliary body thickness between eyes with primary angle closure (PAC) and primary angle-closure glaucoma (PACG) with the normal eyes, and to investigate the association between ciliary body thickness and ciliary processes situation. METHODS: In this cross-sectional study, 57 patients with PAC/PACG were matched to 57 normal subjects after propensity score matching (PSM) adjusting for age and gender. All subjects underwent conventional ocular examinations and ultrasound biomicroscopy (UBM) examination, among which the patients with PAC/PACG performed the examinations one month after laser peripheral iridotomy (LPI). Quantitative parameters were measured, which included ciliary body thickness at the position of 1 mm posterior to the scleral spur (CBT1), trabecular-ciliary process distance (TCPD) and trabecular-ciliary process angle (TCA). RESULTS: Eyes with PAC/PACG presented significantly thinner CBT1, shorter TCPD and smaller TCA (P<0.001) than the normal eyes, both in comparison of the means of four quadrants and in comparisons of each quadrant. After removing images with peripheral anterior synechia (PAS), the same results were also found in comparisons between the two groups. Significant correlations were found between TCPD (R 2=0.537, P<0.001) and TCA (R 2=0.517, P<0.001) with CBT1. CONCLUSION: Eyes with PAC/PACG have thinner ciliary body thickness and more anteriorly situated ciliary processes. Thinner ciliary body thickness is associated with anterior situation of the ciliary processes.

6.
Pest Manag Sci ; 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36129097

RESUMO

BACKGROUND: Insect neuropeptides control essential physiological metabolic activities. In our previous studies, Capability/CAP2b (PK/CAPA) analog 1895 applied alone or as a combination of CAPA analogs (1895+2315) was reported to decrease aphid fitness. While this was obtained with the combination of two peptide analogs of the same neuropeptide class, the effect of combining peptide analogs of different neuropeptide classes has not been explored so far. RESULTS: In this study, we assessed the effect of combinations of the PK/CAPA analog 1895 with neuropeptide analogs of four different classes (adipokinetic hormones analog: 2271; myosuppressin analog: 2434; kinin analog: 2460; tachykinin-related peptide analog: 2463) on the fitness of aphids. We found that the combination of 1895 and AKH analog 2271 was the most effective one to control Myzus persicae. The triple combination 1895+2271+2315 provided a synergistic effect by further increasing aphid mortality and reducing reproduction relative to 1895+2315. Additionally, a biosafety assessment of the combination 1895+2271+2315 showed no significant lethal nor sub-lethal effects on survival rates and food intake for the pollinator (Bombus terrestris) and the two representative natural enemies (Harmonia axyridis and Nasonia vitripennis). CONCLUSION: These results could facilitate establishment of the triple combination 1895+2315+2271, and/or inclusion of 2nd generation analogs, as alternatives to broad spectrum and less friendly insecticides. This article is protected by copyright. All rights reserved.

8.
World J Emerg Med ; 13(5): 379-385, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119773

RESUMO

BACKGROUND: Exosomes and exosomal microRNAs have been implicated in tumor occurrence and metastasis. Our previous study showed that microRNA-761 (miR-761) is overexpressed in hepatocellular carcinoma (HCC) tissues and that its inhibition affects mitochondrial function and inhibits HCC metastasis. The mechanism by which exosomal miR-761 modulates the tumor microenvironment has not been elucidated. METHODS: Exosomal miR-761 was detected in six cell lines. Cell counting kit-8 (CCK-8) and transwell migration assays were performed to determine the function of exosomal miR-761 in HCC cells. The luciferase reporter assay was used to analyze miR-761 target genes in normal fibroblasts (NFs). The inhibitors AZD1480 and C188-9 were employed to determine the role of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in the transformation of cancer-associated fibroblasts (CAFs). RESULTS: In this study, we characterized the mechanism by which miR-761 reprogrammed the tumor microenvironment. We found that HCC-derived exosomal miR-761 was taken up by NFs. Moreover, HCC exosomes affected the tumor microenvironment by activating NFs via suppressor of cytokine signaling 2 (SOCS2) and the JAK2/STAT3 signaling pathway. CONCLUSIONS: These results demonstrated that exosomal miR-761 modulated the tumor microenvironment via SOCS2/JAK2/STAT3 pathway-dependent activation of CAFs. Our findings may inspire new strategies for HCC prevention and therapy.

9.
Anal Methods ; 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36124761

RESUMO

In the egg market, due to the different nutritional values of eggs laid by hens under different feeding conditions, it is common for low-quality eggs to be counterfeited as high-quality eggs. This paper proposes a residual dense comprehensively regulated convolutional neural network (RDCR-Net) to identify the quality of eggs laid by hens under different feeding conditions. Firstly, a hyperspectral system is used to obtain the spectral information of eggs. Secondly, due to the complex structure of the spectral information, a comprehensively regulated convolution (CRConv) is proposed to extract features hidden in the spectral information through feature transformation in multiple spaces. Thirdly, due to the limited availability of spectral information training samples, deep networks may suffer from feature degradation. The residual dense comprehensively regulated block (RDCR-Block) is proposed to tightly connect multiple CRConv layers with residual dense connections. Finally, the RDCR-Block is taken as the central unit, and the RDCR-Net is designed to identify egg spectral information. In the comparison of multi-model results, the RDCR-Net obtains the best classification performance with 96.29% accuracy, 97.53% precision, 97.14% recall, and 96.19% kappa coefficient. In summary, the RDCR-Net effectively extracts the deep features of spectral information, achieves high accuracy in identifying eggs laid by hens under different feeding conditions, and provides a new method for egg quality traceability.

10.
BMC Anesthesiol ; 22(1): 296, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36114451

RESUMO

BACKGROUND: This study was designed to examine extubation time and to determine its association with postoperative pneumonia (POP) after meningioma resection. METHODS: We studied extubation time for 598 patients undergoing meningioma resection from January 2016 to December 2020. Extubation time was analysed as a categorical variable and patients were grouped into extubation within 21 minutes, 21-35 minutes and ≥ 35 minutes. Our primary outcome represented the incidence of POP. The association between extubation time and POP was assessed using multivariable logistic regression mixed-effects models which adjusted for confounders previously reported. Propensity score matching (PSM) was also performed at a ratio of 1:1 to minimize potential bias. RESULTS: Among 598 patients (mean age 56.1 ± 10.7 years, 75.8% female), the mean extubation time was 32.4 minutes. Extubation was performed within 21 minutes (32.4%), 21-35 minutes (31.2%) and ≥ 35 minutes (36.4%), respectively, after surgery. Older patients (mean age 57.8 years) were prone to delayed extubation (≥ 35 min) in the operating room, and more inclined to perioperative fluid infusion. When extubation time was analysed as a continuous variable, there was a U-shaped relation of extubation time with POP (P for nonlinearity = 0.044). After adjustment for confounders, extubation ≥35 minutes was associated with POP (odds ratio [OR], 2.73 95% confidence interval [CI], 1.36 ~ 5.47). Additionally, the results after PSM were consistent with those before matching. CONCLUSIONS: Delayed extubation after meningioma resection is associated with increased pneumonia incidence. Therefore, extubation should be performed as early as safely possible in the operation room.


Assuntos
Neoplasias Meníngeas , Meningioma , Pneumonia , Idoso , Extubação/efeitos adversos , Feminino , Humanos , Masculino , Neoplasias Meníngeas/complicações , Neoplasias Meníngeas/cirurgia , Meningioma/complicações , Meningioma/cirurgia , Pessoa de Meia-Idade , Pneumonia/epidemiologia , Pneumonia/etiologia , Estudos Retrospectivos , Fatores de Risco , Fatores de Tempo
11.
Artigo em Inglês | MEDLINE | ID: mdl-36099220

RESUMO

Toward the development of effective and efficient brain-computer interface (BCI) systems, precise decoding of brain activity measured by an electroencephalogram (EEG) is highly demanded. Traditional works classify EEG signals without considering the topological relationship among electrodes. However, neuroscience research has increasingly emphasized network patterns of brain dynamics. Thus, the Euclidean structure of electrodes might not adequately reflect the interaction between signals. To fill the gap, a novel deep learning (DL) framework based on the graph convolutional neural networks (GCNs) is presented to enhance the decoding performance of raw EEG signals during different types of motor imagery (MI) tasks while cooperating with the functional topological relationship of electrodes. Based on the absolute Pearson's matrix of overall signals, the graph Laplacian of EEG electrodes is built up. The GCNs-Net constructed by graph convolutional layers learns the generalized features. The followed pooling layers reduce dimensionality, and the fully-connected (FC) softmax layer derives the final prediction. The introduced approach has been shown to converge for both personalized and groupwise predictions. It has achieved the highest averaged accuracy, 93.06% and 88.57% (PhysioNet dataset), 96.24% and 80.89% (high gamma dataset), at the subject and group level, respectively, compared with existing studies, which suggests adaptability and robustness to individual variability. Moreover, the performance is stably reproducible among repetitive experiments for cross-validation. The excellent performance of our method has shown that it is an important step toward better BCI approaches. To conclude, the GCNs-Net filters EEG signals based on the functional topological relationship, which manages to decode relevant features for brain MI. A DL library for EEG task classification including the code for this study is open source at https://github.com/SuperBruceJia/ EEG-DL for scientific research.

12.
J Environ Manage ; 321: 115982, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36104886

RESUMO

Hydrodynamic cavitation has been a promising method and technology in wastewater treatment, while the principles based on the design of cavitational reactors to optimize cavitation yield and performance remains lacking. Computational fluid dynamics (CFD), a supplementation of experimental optimization, has become an essential tool for this issue, owing to the merits of low investment and operating costs. Nevertheless, researchers with a non-engineering background or few CFD fundamentals used straightforward numerical strategies to treat cavitating flows, and this might result in many misinterpretations and consequently poor computations. This review paper presents the rationale behind hydrodynamic cavitation and application of cavitation modeling specific to the reactors in wastewater treatment. In particular, the mathematical models of multiphase flow simulation, including turbulence closures and cavitation models, are comprehensively described, whilst the advantages and shortcomings of each model are also identified and discussed. Examples and methods of the coupling of CFD technology, with experimental observations to investigate into the hydrodynamic behavior of cavitating devices that feature linear and swirling flows, are also critically summarized. Modeling issues, which remain unaddressed, i.e., the implementation strategies of numerical models, and the definition of cavitation numbers are identified and discussed. Finally, the advantages of CFD modeling are discussed and the future of CFD applications in this research area is also outlined. It is expected that the present paper would provide decision-making support for CFD beginners to efficiently perform CFD modeling and promote the advancement of cavitation simulation of reactors in the field of wastewater treatment.


Assuntos
Hidrodinâmica , Purificação da Água , Simulação por Computador , Modelos Teóricos , Tecnologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-36112494

RESUMO

Block-copolymer-derived ordered mesoporous carbon (OMC) materials have great potential in many applications, such as adsorption, catalysis, and energy conversions; however, their formation process and the kinetic mechanism remain unclear. Herein, a N-doped OMC (N-OMC) with sp2-bonded C atoms is developed via self-assembly of the polystyrene-block-poly(4-vinyl pyridine) block copolymer. By correlating the external morphologies with the internal chemical states, the formation process can be concluded as follows: (1) pore evolution via polystyrene domain degradation and (2) regularization and graphitization of the residual carbon via the removal of sp3 C atoms. In addition, the thickness of the N-OMC shows a power function relationship with the spin-coating rate, and the N content can be incredibly increased up to 26.34 at. % in an NH3 carbonization atmosphere. With the as-prepared N-OMC as the support for loading of the pseudo-atomic-scale Pt (Pt/N-OMC), a high electrochemical active surface area value of 99.64 m2·g-1 and a half-wave potential (E1/2) of 0.850 VRHE are achieved, showing great potential in developing single-atom electrocatalysts.

14.
Neurochem Res ; 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36063295

RESUMO

Lead (Pb) is considered to be a major environmental pollutant and occupational health hazard worldwide which may lead to neuroinflammation. However, an effective treatment for Pb-induced neuroinflammation remains elusive. The aim of this study was to investigate the mechanisms of Pb-induced neuroinflammation, and the therapeutic effect of sodium para-aminosalicylic acid (PAS-Na, a non-steroidal anti-inflammatory drug) in rat cerebral cortex. The results indicated that Pb exposure induced pathological damage in cerebral cortex, accompanied by increased levels of inflammatory factors tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1ß). Moreover, Pb decreased the expression of silencing information regulator 2 related enzyme 1 (SIRT1) and brain-derived neurotrophic factor (BDNF), and increased the levels of high mobile group box 1 (HMGB1) expression and p65 nuclear factor-κB (NF-κB) phosphorylation. PAS-Na treatment ameliorated Pb-induced histopathological changes in rat cerebral cortex. Moreover, PAS-Na reduced the Pb-induced increase of TNF-α and IL-1ß levels concomitant with a significant increase in SIRT1 and BDNF levels, and a decrease in HMGB1 and the phosphorylation of p65 NF-κB expression. Thus, PAS-Na may exert anti-inflammatory effects by mediating the SIRT1/HMGB1/NF-κB pathway and BDNF expression. In conclusion, in this novel study PAS-Na was shown to possess an anti-inflammatory effect on cortical neuroinflammation, establishing its efficacy as a potential treatment for Pb exposures.

15.
Cell Mol Biol Lett ; 27(1): 72, 2022 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-36058940

RESUMO

Osteoporotic fractures lead to increased disability and mortality in the elderly population. With the rapid increase in the aging population around the globe, more effective treatments for osteoporosis and osteoporotic fractures are urgently required. The underlying molecular mechanisms of osteoporosis are believed to be due to the increased activity of osteoclasts, decreased activity of osteoblasts, or both, which leads to an imbalance in the bone remodeling process with accelerated bone resorption and attenuated bone formation. Currently, the available clinical treatments for osteoporosis have mostly focused on factors influencing bone remodeling; however, they have their own limitations and side effects. Recently, cytokine immunotherapy, gene therapy, and stem cell therapy have become new approaches for the treatment of various diseases. This article reviews the latest research on bone remodeling mechanisms, as well as how this underpins current and potential novel treatments for osteoporosis.


Assuntos
Reabsorção Óssea , Osteoporose , Fraturas por Osteoporose , Idoso , Remodelação Óssea , Reabsorção Óssea/complicações , Reabsorção Óssea/terapia , Humanos , Osteoclastos , Osteoporose/tratamento farmacológico , Fraturas por Osteoporose/complicações
16.
Front Aging Neurosci ; 14: 966647, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072484

RESUMO

Objectives: Studies have shown that the frequent participation of the elderly in cognitive stimulation activities is associated with a reduced risk of dementia, but the prospective evidence of this association is limited. Methods: We used data from a prospective cohort study of the Chinese Longitudinal Healthy Longevity Survey (CLHLS), and included 11,821 community-living Chinese individuals aged 65 years or older at 2008 baseline who were free of dementia, and were followed up every 2-3 years until 2018. Cox proportional hazards models were applied to generate the hazard ratios (HRs) and 95% confidence intervals (CIs) for analyzing the associations between the frequency of playing cards/mahjong and the incidence of dementia. Results: A total of 821 participants were diagnosed with dementia during the 10-year follow-up. The average age of patients with dementia and non dementia were 89 and 90 years old, respectively. Compared with participants who rarely or never played cards/mahjong, participants who played cards/mahjong almost every day had a significantly lower risk of dementia (HR = 0.63; 95%CI, 0.42-0.95) after the multivariable-adjusted model. Similar results were observed in subgroup analyses based on sex (male: HR = 0.52, 0.28-0.96; female: HR = 0.62, 0.36-0.98), age (<85years: HR = 0.55, 0.32-0.89), regularly exercise (yes: HR = 0.44, 0.28-0.87) and MMSE score [above median (25): HR = 0.66, 0.41-0.92]. Conclusions: Playing cards/mahjong in the elderly may contribute to reducing the risk of dementia.

17.
Front Public Health ; 10: 980465, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36062100

RESUMO

Objective: Disability and social support can impact depressive symptoms of the elderly. Yet, studies infrequently discuss the moderating role of social support when evaluating the association between disability and depressive symptoms. The purpose of this study was to explore the association between disability, social support, and depressive symptoms among the Chinese elderly, and further examine the moderating effect of social support. Materials and methods: Using the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) data set, we finally selected 9,231 Chinese elderly after screening. The Center for Epidemiologic Studies Depression Scale (CESD-10) was used to evaluate depressive symptoms in the elderly. Disability was measured by basic activities of daily living (B-ADL) and instrumental activities of daily living (I-ADL). Social support included contact with family and friends, sick care, and money received, measured by five self-reported questions. We used multiple linear regression and moderating model to explore the association between disability, social support, and depressive symptoms. Results: A total of 9,231 patients were included in this study, and approximately 26.75% of the elderly had depressive symptoms. Study found that depressive symptoms were associated with social support (ß B-ADL = -0.108, 95% CI: -0.168- -0.047; ß I-ADL = -0.098, 95% CI: -0.156- -0.039), ß B-ADL (ß = 0.296, 95% CI: 0.248-0.343) and I-ADL (ß = 0.174, 95% CI: 0.152-0.195). Moreover, the result also showed that social support moderated the effects of B-ADL ( ß B - A D L * s o c i a l s u p p o r t = 0.034, 95% CI: 0.014-0.053, F = 11.57, p = 0.001) and I-ADL ( ß I - A D L * s o c i a l s u p p o r t = 0.025, 95% CI: 0.017-0.033) on depressive symptoms. Conclusions: The study suggests that disability and social support can affect depressive symptoms, and social support moderates the effect of disability on depressive symptoms. Therefore, taking effective measures to reduce the elderly disability rate of disability and increase their social support are necessary condition for realizing mental health.


Assuntos
Atividades Cotidianas , Pessoas com Deficiência , Atividades Cotidianas/psicologia , Idoso , China/epidemiologia , Depressão/epidemiologia , Depressão/psicologia , Pessoas com Deficiência/psicologia , Humanos , Apoio Social
18.
Metabolites ; 12(9)2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36144229

RESUMO

Metabolism-associated fatty liver disease (MAFLD) is one of the most common causes of liver disease; however, the underlying processes remain unknown. This study aimed to investigate the changes of free fatty acids (FFA) on the expression of genes related to the AMP-activated protein kinase (AMPK) signaling pathway in the primary hepatocytes of laying hens. The primary hepatocytes of laying hens were treated with FFA (containing a 2:1 ratio of oleic and palmitic acids) for 24 h. FFA significantly increased lipid droplet accumulation, decreased glycogen synthesis, increased the levels of triglycerides (TG), total cholesterol (TC), reactive oxygen species (ROS), malondialdehyde (MDA), and glucose content in the supernatant (GLU) in the primary hepatocytes of laying hens, and decreased the levels of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD), as well as mitochondrial membrane potential (MMP). The results of the PCR array combined with Western blotting experiments showed that the activity of AMPK was inhibited. Inhibition of AMPK signaling pathway decreases the expression of genes involved in fatty acid oxidation, increases the expression of genes involved in lipid synthesis, decreases the expression of genes involved in glycogen synthesis, increases the expression of genes involved in glycolysis, increases the expression of genes involved in oxidative stress, and increases the expression of genes involved in cell proliferation and apoptosis. Taken together, our results suggest that FFA can affect the homeostasis of the AMPK signaling pathway by altering energy metabolic homeostasis, inducing oxidative stress, and adjusting the onset of cell proliferation and apoptosis.

19.
Front Pharmacol ; 13: 893758, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36059974

RESUMO

Gut homeostasis is important for human health, and its disruption can lead to inflammatory bowel disease (IBD). Flos Puerariae is a herb with a wide variety of pharmacological activities including antioxidant, antidiabetic, antialcoholismic and anti-inflammatory properties. However, the role of Flos Puerariae on treating IBD remains obscure. Here, we employed Drosophila melanogaster as a model organism to investigate the protective effect of Flos Puerariae extract (FPE) against sodium dodecyl sulfate (SDS)-induced intestinal injury. Our data showed that FPE had no toxic effect in flies, and significantly extended lifespan in SDS-inflamed flies, reduced stem cell proliferation in the midgut, and maintained intestinal morphological integrity. Furthermore, FPE remarkably recused the altered expression level of genes and proteins in Nrf2/Keap1 signaling, JAK-STAT signaling and Wnt signaling pathways in gut of inflammation flies. Thus, FPE has a protective effect against intestinal injury possibly via increasing the Nrf2/keap1 pathway and suppressing the JAK-STAT and Wnt signaling pathways, which would have tremendous potential for treating IBD.

20.
Ann Neurol ; 2022 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-36088542

RESUMO

OBJECTIVE: Despite the increasing number of genes associated with Charcot-Marie-Tooth (CMT) disease, many patients currently still lack appropriate genetic diagnosis for this disease. Autosomal dominant mutations in aminoacyl-tRNA synthetases (ARSs) have been implicated in CMT. Here, we describe causal missense mutations in the gene encoding Seryl-tRNA synthetase 1 (SerRS) for three families affected with CMT. METHODS: Whole-exome sequencing was performed in 16 patients and 14 unaffected members of three unrelated families. The functional impact of the genetic variants identified was investigated using bioinformatic prediction tools and confirmed using cellular and biochemical assays. RESULTS: Combined linkage analysis for the three families revealed significant linkage (Zmax LOD = 6.9) between the genomic co-ordinates on chromosome 1: 108681600-110300504. Within the linkage region, heterozygous SerRS missense variants segregated with the clinical phenotype in the three families. The mutant SerRS proteins exhibited reduced aminoacylation activity and abnormal SerRS dimerization, which suggests the impairment of total protein synthesis and induction of eIF2α phosphorylation. INTERPRETATION: Our findings suggest the heterozygous SerRS variants identified represent a novel cause for autosomal dominant CMT. Mutant SerRS proteins are known to impact various molecular and cellular functions. Our findings provide significant advances on the current understanding of the molecular mechanisms associated with ARS-related CMT. This article is protected by copyright. All rights reserved.

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