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Plant Physiol Biochem ; 125: 205-211, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29475086


Siliceous frustules of diatom algae contain unique long-chain polyamines, including those having more than six nitrogen atoms. These polyamines participate in the formation of the siliceous frustules of the diatoms but their precise physiological role is not clear. The main hypotheses include formation of a polyamine and polyphosphate supramolecular matrix. We have synthesized novel fluorescent dyes from a synthetic oligomeric mixture of polyamines and the fluorophore 7-nitro-2,1,3-benzoxadiazole. The long polyamine chain ensures the high affinity of these dyes to silica, which allows their application in the staining of siliceous materials, such as valves of diatom algae and fossilized samples from sediments. The fluorescently stained diatom valves were found to be promising liquid flow tracers in hydrodynamic tests. Furthermore, complexation of the polyamine component of the dyes with carbonic polymeric acids results in changes to the visible spectrum of the fluorophore, which allows study of the stability of the complex vs the length of the polyamine chain. Using poly (vinyl phosphonic acid) as a model for phosphate functionality in silaffins (a potential matrix in the formation of biogenic silica) little complexation with the polyamine fluorophores was observed, bringing into question the role of a polyamine - polymeric phosphate matrix in biosilicification.

4-Cloro-7-nitrobenzofurazano/química , Poliaminas Biogênicas , Diatomáceas , Corantes Fluorescentes/química , Coloração e Rotulagem/métodos , Poliaminas Biogênicas/química , Poliaminas Biogênicas/metabolismo , Diatomáceas/citologia , Diatomáceas/metabolismo
Nat Prod Res ; 31(6): 639-644, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27486824


The potential antibacterial activity of secondary metabolites produced by Streptomyces sp. 156A isolated from Lake Baikal was investigated. The selective liquid-liquid extraction method was applied to obtain a mixture of nactins (polynactin) produced by the strain. The polynactin consisted of nonactin (3%), monactin (18%), dinactin (36%), trinactin (31%) and tetranactin (12%). The compounds were identified by MS/MS, 1H and 13C NMR methods. The loss of neutral 184 and 198 Da fragments from a sodiated molecular ion, [M + Na]+, of nactins was observed in the MS/MS spectrum. The polynactin was shown to possess the antibiotic activity against Gram-positive strains including opportunistic strains and strains isolated from various ecosystems of Lake Baikal.

Antibacterianos/farmacologia , Macrolídeos/farmacologia , Streptomyces/química , Antibacterianos/biossíntese , Farmacorresistência Bacteriana/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Lagos/microbiologia , Extração Líquido-Líquido , Macrolídeos/química , Testes de Sensibilidade Microbiana , Federação Russa , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Streptomyces/isolamento & purificação , Espectrometria de Massas em Tandem
Anal Biochem ; 486: 5-13, 2015 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-26103595


4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) is widely applied as a fluorescent tagging reagent in biochemistry, as a derivatization agent in analytical chemistry, and as a component for design of fluorescent nanoparticles. Four new 7-nitrobenzo-2-oxa-1,3-diazole (NBD)-tagged polyamines containing two to four amine moieties were synthesized and used as an effective tool for staining of siliceous frustules of the diatom algae and spicules of the siliceous sponges, including fossilized samples. An unexpected reaction between NBD-Cl and tertiary amine groups was found, giving rise to NBD-tagged amines with elimination of an alkyl group. The reaction proceeds through the Meisenheimer complex and quaternary salt, which transform to the product by Hofmann reaction (alkene elimination) or nucleophilic substitution (halogenated compound formation). In the case of polyamines, NBD-Cl causes chain scissoring, giving a set of NBD-tagged amines. The found NBD-Cl reaction with tertiary amines must be taken into account when using NBD-Cl and similar activated aromatic systems for amine derivatization in analytical and biochemistry applications. The reaction with polyamines opens the way to libraries of NBD-tagged compounds.

4-Cloro-7-nitrobenzofurazano/química , Corantes Fluorescentes/química , Poliaminas/química , 4-Cloro-7-nitrobenzofurazano/síntese química , Animais , Diatomáceas/química , Corantes Fluorescentes/síntese química , Poríferos/química , Coloração e Rotulagem