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1.
Aust Endod J ; 2021 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-33754413

RESUMO

This study evaluated the ability of reciprocating glide path instruments to reach the full working length (RFWL). Thirty mesial roots of mandibular molars were matched into 2 groups (n = 15 teeth, 30 root canals), according to the system used for glide path: WaveOne Gold Glider (WOGG) and R-Pilot. The samples with fractured instruments or when it was not possible to RFWL were scanned using micro-computed tomography. Fractured instruments were also visualised through a scanning electron microscopy to verify the type of fracture. The parameters were compared statistically by Fisher's and chi-square tests (P < 0.05). No instrument fractures were observed in the R-pilot, while 2 fractures occurred in the WOGG group (P > 0.05). RFLW was achieved in 29 canals (96.66%) and 28 canals (93.33%) with R-pilot and WOGG instruments, respectively (P > 0.05). The instruments are able to RFWL in mesial canals of mandibular molars. WOGG showed two fractures while no R-Pilot instruments fractured.

2.
Dent Mater ; 37(5): 863-874, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33648745

RESUMO

OBJECTIVE: The aim of this study was to evaluate the influence of the incorporation of chlorhexidine-hexametaphosphate nanoparticles (CHX-HMP NPs) on antibacterial, cytotoxic and physicochemical properties of AH Plus (AH), MTA Fillapex (MTA) and Pulp Canal Sealer (PCS). METHODS: The NPs were synthesized and characterized by Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), zeta potential, Atomic Force Microscopy (AFM) and Energy-Dispersive X-ray Spectroscopy (EDS). The incorporation was made by weight, 2% and 5% of NPs. The antimicrobial activity, cytotoxicity, flow, radiopacity, setting time, solubility and pH were evaluated. The statistical analysis was performed by two-way analysis of variance test and Tukey post hoc test (P < 0.05). RESULTS: SEM analysis showed the tendency for CHX-HMP NPs to cluster, the effective mean diameter measured by DLS: 169.39 nm and the zeta potential: -10.18 mV. The NPs were individually measured by AFM: 22.99-52.75 nm. EDS analysis identified the presence of C, N, O, Na, P, Cl. After incorporation: The Direct Contact Test showed an increase in the antimicrobial action of AH, PCS and MTA; the sealers showed a decrease in flow and at 24 h of immersion also an increase in solubility, but did not affect the radiopacity of the samples; AH setting time increased and MTA did not reach setting under any of the conditions tested. All samples showed a decrease in pH value as the immersion time progressed. SIGNIFICANCE: The incorporation of NPs can improve the antimicrobial performance of endodontic sealers without impairing other biological and physicochemical properties.


Assuntos
Nanopartículas , Materiais Restauradores do Canal Radicular , Antibacterianos/farmacologia , Compostos de Cálcio , Clorexidina/farmacologia , Cavidade Pulpar , Combinação de Medicamentos , Resinas Epóxi , Teste de Materiais , Fosfatos , Silicatos
3.
Braz Oral Res ; 35: e021, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33605352

RESUMO

The aim of the present study was to evaluate the cyclic fatigue, torsional resistance and surface roughness of Reciproc R25 instruments in four different situations, namely as new instruments and as instruments tested after clinical preparation of one, two or three maxillary molars with four root canals. The total time required to perform each root canal preparation was recorded. Cyclic fatigue resistance was determined by the time to fracture using a customized testing device (n = 10 per group). The torsional test evaluated the torque and angle of rotation to failure according to ISO 3630-1 (n = 10 per group). The roughness of the working parts of new and used instruments was evaluated with a profilometer (n = 5 per group). Statistical analysis was performed using one-way ANOVA and Tukey's test. The level of significance was set at 5%. No fractures or deformations were observed after clinical use. Higher preparation time was needed during the third use of the instruments for all root canals (p < 0.05). There were no significant differences among the groups in regard to either cyclic fatigue or torsional resistance (p > 0.05). Regarding the roughness measurements, groove depth was higher on new and one- versus two- or three-maxillary-molar-prepared instruments (p < 0.05). It can be concluded that the clinical use of Reciproc instruments increased preparation time and decreased surface roughness. However, clinical use did not affect the cyclic fatigue or torsional resistance of the Reciproc instruments.


Assuntos
Preparo de Canal Radicular , Titânio , Instrumentos Odontológicos , Desenho de Equipamento , Teste de Materiais , Estresse Mecânico , Torque
4.
Aust Endod J ; 47(1): 81-89, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33368843

RESUMO

This study aimed to evaluate the influence of large apical preparations with Reciproc (REC), Hyflex CM (HCM) and Twisted File Adaptive (TFA) systems using micro-computed tomography (MCT). Ninety mesiobuccal (MB) and distobuccal (DB) root canals of maxillary molars (n = 45) were scanned using MCT before and after the shaping procedures. The root canals (n = 15) were prepared until REC 40.06, HCM 40.04 and TFA 35.04. The root canal transportation (RCT), centring ability (CA), change in volume of the root canal and at different levels (VC), remaining dentine thickness (RDT), removal of dentine wall (RDW) and working time (WT) were evaluated. Data were analysed using the Kruskal-Wallis and Dunn tests, and the one-way ANOVA and Tukey tests with a level of significance set at 5%. No significant difference among the instruments was found regarding the RCT, CA, RDT, RDW and WT (P > 0.05), in larger apical preparations in curved MB and straight DB canals of maxillary molars.


Assuntos
Cavidade Pulpar , Preparo de Canal Radicular , Cavidade Pulpar/diagnóstico por imagem , Desenho de Equipamento , Dente Molar/diagnóstico por imagem , Microtomografia por Raio-X
5.
Braz. oral res. (Online) ; 35: e021, 2021. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1153624

RESUMO

Abstrac The aim of the present study was to evaluate the cyclic fatigue, torsional resistance and surface roughness of Reciproc R25 instruments in four different situations, namely as new instruments and as instruments tested after clinical preparation of one, two or three maxillary molars with four root canals. The total time required to perform each root canal preparation was recorded. Cyclic fatigue resistance was determined by the time to fracture using a customized testing device (n = 10 per group). The torsional test evaluated the torque and angle of rotation to failure according to ISO 3630-1 (n = 10 per group). The roughness of the working parts of new and used instruments was evaluated with a profilometer (n = 5 per group). Statistical analysis was performed using one-way ANOVA and Tukey's test. The level of significance was set at 5%. No fractures or deformations were observed after clinical use. Higher preparation time was needed during the third use of the instruments for all root canals (p < 0.05). There were no significant differences among the groups in regard to either cyclic fatigue or torsional resistance (p > 0.05). Regarding the roughness measurements, groove depth was higher on new and one- versus two- or three-maxillary-molar-prepared instruments (p < 0.05). It can be concluded that the clinical use of Reciproc instruments increased preparation time and decreased surface roughness. However, clinical use did not affect the cyclic fatigue or torsional resistance of the Reciproc instruments.

6.
J Clin Exp Dent ; 12(11): e1058-e1065, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33262872

RESUMO

Background: To evaluate the physicochemical properties and cytotoxicity of AH Plus, MTA Fillapex and TotalFill BC Sealer. Volumetric changes were also evaluating using micro-computed tomography (micro-CT). Material and Methods: Radiopacity and flow were evaluated in accordance with the ISO 6876, while setting time was evaluated in accordance with the ASTM- C266-08 specifications. The release of Ca2+ ions and pH were measured with spectrophotometer and pH meter, respectively, after different time intervals (1h, 3h, 24h, 72h, 168h, and 360h). Cytotoxicity was evaluated by MTT reduction assay to check 3T3 cells viability at 24, 48 and 72 hours. Volumetric change was evaluated by micro-CT, by using 30 acrylic teeth, filled with gutta-percha cones and the tested root canal sealer. The samples were evaluated after 168h, 360h and 720h of immersion in distilled water. Data were statistically analyzed by one-way ANOVA and Tukey test or by Kruskal-Wallis and Dunn tests (P<0.05). Results: MTA Fillapex and TotalFill BC Sealer showed lower radiopacity than AH Plus (P<0.05). The MTA Fillapex showed the highest flow, while AH Plus showed the lowest flow (P<0.05). The initial and final setting time of AH Plus were lower than MTA Fillapex and TotalFill BC Sealer (P<0.05). In general, TotalFill BC Sealer presented higher Ca2+ ion release and pH than the other tested sealers. TotalFill BC Sealer also showed overall lower cytotoxicity when compared to the other sealers. Volumetric change of AH Plus and TotalFill BC Sealer was lower than MTA Fillapex (P<0.05). Conclusions: AH Plus, MTA Fillapex and TotalFill BC Sealer showed slight differences in the physicochemical properties and cytotoxicity, but all suitable for an endodontic sealer. However, AH Plus and TotalFill BC Sealer showed low volumetric changes when compared to MTA Fillapex. Key words:Calcium silicate, cytotoxicity, physicochemical properties, micro computed tomography.

7.
Clin Oral Investig ; 2020 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-33219876

RESUMO

INTRODUCTION: The aim of the present study was to compare the cyclic fatigue resistance of Reciproc R25 (R25) and Reciproc Blue R25 (R25B) instruments, after simulated clinical use in traditional (TradAC) and ultraconservative (UltraAC) endodontic access cavities. METHODS: Forty mandibular molars were randomly assigned into the following groups, according to the type of access and instrument to be used: TradAC and R25, TradAC and R25B, UltraAC and R25, and UltraAC and R25B. Teeth were accessed accordingly, and the root canals were prepared using "RECIPROC ALL" kinematics. The cyclic fatigue resistance of the forty used instruments was obtained measuring the time to fracture in an artificial stainless-steel canal. Ten brand new R25 and R25B were used as control groups. The fracture surfaces and the side cutting edges of the instruments were examined with a scanning electron microscope. Data were statistically analyzed using one-way ANOVA and post hoc Tukey tests with a significance level of P < 0.05. RESULTS: R25B instruments showed significantly higher cyclic fatigue resistance than R25, regardless of the access cavity type (P < 0.05). No differences were observed in the cyclic fatigue resistance between instruments without simulated clinical use and used in TradAC (P > 0.05). R25 and R25B used in UltraAC showed significantly lower cyclic fatigue resistance compared with the instruments used in TradAC and without simulated clinical use (P < 0.05). CONCLUSIONS: R25B files showed improved cyclic fatigue resistance than R25. The use of R25B and R25 files in mandibular molars with UltraACs decreased their cyclic fatigue resistance, compared with TradAC. CLINICAL RELEVANCE: The use of Reciproc and Reciproc Blue files in mandibular molars with ultra-conservative endodontic access cavities reduced their cyclic fatigue resistance. Clinicians should be aware about the reduced cyclic fatigue resistance of these files when used in mandibular molars with UltraAC, due to the synergistic effect of access angulation and severe curvature induced in the endodontic files.

8.
Clin Oral Investig ; 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33236240

RESUMO

OBJECTIVES: This study evaluated the danger zone (DZ) in mesial roots of mandibular molars and the correlation between anatomical references of the DZ and some anatomical landmarks including tooth/root length, depth of mesial and distal grooves, and inter-canal orifices distance. MATERIAL AND METHODS: Twenty-eight mesial roots of mandibular molars with 2 independent canals were scanned and divided into 2 groups according to root length. The anatomical landmarks were correlated (Pearson or Spearman coefficients) with root level, thickness, and position of the DZ and also compared (independent samples t or Mann-Whitney tests) between the 2 groups at α = 5%. RESULTS: No statistical difference was observed between groups regarding DZ parameters and depth of mesial and distal grooves (P > 0.05). Orifice distance in group 2 (4.49 ± 0.75 mm) was significantly greater than group 1 (3.76 ± 0.89 mm) (P < 0.05). Significant correlations (P < 0.05) were found between (i) DZ level and root/tooth length (r = 0.54 and 0.49, respectively), (ii) DZ thickness and distal groove depth (r = - 0.45), and orifice distance (r = 0.38), and (iii) DZ position and depth of mesial (r = 0.39) and distal (r = 0.40) grooves. Other variables such as root length and distal groove depth (r = 0.28), and orifice distance and mesial groove depth (r = 0.36) were also correlated (P < 0.05). CONCLUSIONS: The length of tooth/root, the distance of canal orifices, and the depth of mesial/distal grooves of mesial roots of mandibular molars might be predictive factors for the root level, position, and thickness of the DZ. CLINICAL RELEVANCE: The length, distance of mesial canal orifices, and the depth of mesial and distal grooves of the mesial roots of mandibular molars might be moderate predictive factors for the root level, position, and thickness of the DZ.

9.
J Endod ; 2020 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-32818567

RESUMO

INTRODUCTION: The aim of this study was to evaluate the cyclic fatigue resistance of different heat-treated nickel-titanium reciprocating instruments in 2 different situations: new and used instruments after preparing 3 curved canals. METHODS: A total sample of 60 nickel-titanium instruments of 3 systems (n = 20 per system) were used in this study: ProDesign R (tip 25, 0.06 taper; Easy Dental Equipment, Belo Horizonte, Brazil), Reciproc Blue (tip 25, 0.08v taper; VDW, Munich, Germany), and WaveOne Gold (tip 25, 0.07v taper; Dentsply Sirona, Ballaigues, Switzerland). Thirty new instruments (n = 10 per system) were used to prepare 90 curved single-rooted mandibular premolars (n = 30). Each instrument was used to prepare 3 root canals, and after each canal preparation the instrument was ultrasonic cleaned and submitted to autoclave sterilization procedures. The other 30 instruments (n = 10 per system) were kept without use. Then, the new and used instruments were subjected to the cyclic fatigue test in an artificial canal with a 30° angle and a 5-mm radius of curvature. The time and number of cycles to fatigue were recorded. Data were analyzed using the unpaired t test for intragroup comparison. For intergroup comparison, analysis of variance and the Tukey test for multiple comparisons were used. RESULTS: The intergroup comparison of new instruments showed that ProDesign R had the highest cyclic fatigue resistance followed by Reciproc Blue and WaveOne Gold (P < .05). Regarding the used instruments, WaveOne Gold had the lowest cyclic fatigue resistance (P < .05). The intragroup comparison between new and used instruments showed that WaveOne Gold and ProDesign R presented a significant reduction in the cyclic fatigue resistance after simulated clinical use (P < .05); no difference was found with the Reciproc Blue instruments (P > .05). CONCLUSIONS: ProDesign R had the highest cyclic fatigue resistance, whereas WaveOne Gold had the lowest for new and used instruments. Simulated clinical use affected the cyclic fatigue resistance of ProDesign R and WaveOne Gold but not of Reciproc Blue instruments.

10.
Clin Oral Investig ; 2020 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-32789655

RESUMO

OBJECTIVES: To present and explore the potential of an animal-based experimental model developed to determine the set of root canal sealers in vivo. The setting of AH Plus, BioC Sealer, TotalFill BC Sealer, and Sealapex was determined using either ISO 6876 or the novel in vivo method proposed in this study. MATERIAL AND METHODS: The in vitro setting time of the sealers tested was determined in accordance with ISO 6876:2012. In determining the in vivo set, 24 adult Wistar rats were followed up for two evaluation periods: 1 and 4 weeks. Their upper-right incisor was extracted, and its pulp tissue was removed. The root canal was then filled from retrograde with one of the 4 sealers, and the tooth was re-implanted and fixed with a layer of a flowable composite resin. After 1 or 4 weeks of the surgical procedures, the animals were euthanized, and their incisors were extracted. Two-mm-thick slices of the middle third of the tooth root were obtained and assessed with a Gillmore device, to determine whether or not the sealer had set. RESULTS: The following in vitro results were obtained by using ISO 6876 methodology: AH Plus set after a mean time of 423 ± 20 min and 476 ± 35 min, in metal and plaster molds, respectively. BioC Sealer set after 7 days (in dental plaster molds), whereas TotalFill BC Sealer and Sealapex did not set even after 25 days in both tested conditions (metal or dental plaster molds). Using the novel in vivo methodology, AH Plus, BioC Sealer, and TotalFill BC Sealer set after both 7 and 30 days. In contrast, Sealapex did not set at either time point. CONCLUSIONS: AH Plus and BioC Sealer set under both in vitro and in vivo test conditions. TotalFill BC Sealer did not set under in vitro conditions but did after 1 week under in vivo conditions. Sealapex did not set under either in vitro or in vivo conditions. CLINICAL RELEVANCE: The influence of the testing conditions on the setting results is a clear indication that new in vivo experimental models should be useful in future studies on Bioceramics root canal sealers.

11.
Braz Oral Res ; 34: e069, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32696911

RESUMO

The aim of this randomized clinical trial was to compare the occurrence and intensity of postoperative pain and analgesic intake after root canal treatment, using different root canal sealers. Sixty single-rooted teeth diagnosed with asymptomatic necrosis and apical periodontitis were randomly assigned to 3 experimental groups (n=20), according to the root canal sealer: AH Plus, Endofill or MTA Fillapex. Endodontic treatment was performed in two sessions, and calcium hydroxide was used as the intracanal dressing. Patients were instructed to record pain intensity as none, slight, moderate and severe. Scores from 1 to 4 were attributed to each level of pain after 24 h, 48 h and 7 days. The need for analgesic intake was also recorded. Differences in the incidence of postoperative pain and the need for an analgesic were analyzed using the chi-square test. Differences in pain intensity after treatment were analyzed using the ordinal (linear) chi-square test. No significant differences were detected among the groups in terms of either incidence or intensity of postoperative pain, or need for analgesic intake, at any timepoint (p>0.05). No pain was reported after 7 days. AH Plus, Endofill and MTA Fillapex used for filling root canals resulted in the same rate of postoperative pain and need for analgesic medication.


Assuntos
Cavidade Pulpar , Hidróxido de Cálcio , Resinas Epóxi , Humanos , Dor Pós-Operatória , Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Tratamento do Canal Radicular
12.
J Endod ; 2020 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-32721486

RESUMO

INTRODUCTION: The aim of the present study was to evaluate the morphologic changes on the apical foramen and the formation of dentinal microcracks after foraminal enlargement in straight and curved root canals using scanning electron microscopic (SEM) and micro-computed tomographic (micro-CT) analyses. METHODS: Sixty teeth were selected and divided into 2 experimental groups: GI (incisors/straight group, n = 30) and GM (molars/curved group, n = 30). Each group was divided into 2 subgroups (n = 15) according to the analysis performed: SEM or micro-CT analysis. The incisors and mesiobuccal canal of molars were instrumented with Reciproc Blue R25 instruments (VDW, Munich, Germany) using standardized shaping procedures in the apical foramen and 1 mm beyond. The occurrence of foraminal deformation was analyzed by scanning electron microscopy. The z test was used for statistical analysis (P < .05). Micro-CT imaging was used to observe the presence of microcracks in the root apical third. Afterward, pre- and postoperative cross-sectional images were screened to identify the presence of dentinal defects. RESULTS: The curvature of the canal did not influence the occurrence of foraminal deformation either for instrumentation in the foramen or for instrumentation beyond the foramen (P > .05). It was also observed that the increase in the working length did not influence the appearance of new deformations in the GI/SEM and GM-SEM groups (P > .05). Qualitative micro-CT analysis showed the presence of dentinal defects in 0.8% (112) and 1.5% (208) of the cross sections of incisors and molars, respectively, from a total of 13,987 slices. All dentinal defects identified in the analysis of any postoperative scans were already present in the corresponding preoperative images. Therefore, no new microcracks were observed after root canal preparation. CONCLUSIONS: The preparation of straight and moderately curved root canals with Reciproc Blue, regardless of the working length, did not influence the occurrence of apical foramen deformations and did not cause the formation of dentinal microcracks.

13.
Restor Dent Endod ; 45(2): e25, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32483542

RESUMO

Objectives: This systematic review evaluated the influence of autoclave sterilization procedures on the cyclic fatigue resistance of heat-treated nickel-titanium (NiTi) instruments. Materials and Methods: A systematic search without restrictions was conducted in the following electronic databases: PubMed, Scopus, Web of Science, ScienceDirect, Cochrane, and Open Grey. The hand search was also performed in the main endodontic journals. The eligible studies were submitted to the methodological assessment and data extraction. Results: From 203 abstracts, a total of 10 articles matched the eligible criteria. After reading the full articles, 2 were excluded because of the absence of the heat-treated instruments in the experimental design and 3 due to the lack of a control group using heat-treated instruments without autoclave sterilization. From the 5 included studies, 1 presented a low risk of bias, 3 presented moderate and 1 high risk. It was observed heterogeneous findings in the included studies, with autoclave sterilization cycles increasing, decreasing or not affecting the cyclic fatigue life of heat-treated NiTi instruments. However, the retrieved studies evaluating the cyclic fatigue resistance of endodontic instruments presented different protocols and assessing outcomes, this variability makes the findings less comparable within and also between groups and preclude the establishment of an unbiased scientific evidence base. Conclusions: Considering the little scientific evidence and considerable risk of bias, it is still possible to conclude that autoclave sterilization procedures appear to influence the cyclic fatigue resistance of heat-treated NiTi instruments.

14.
Clin Oral Investig ; 24(9): 3299-3305, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31965283

RESUMO

OBJECTIVES: To assess C-shaped root canal configuration by identifying its prevalence and each configuration type proportion, according to tooth (mandibular first or second molar) and demographic characteristics in a Brazilian population, using cone-beam computed tomography (CBCT). Moreover, it was verified if there is a relationship between root canal configuration observed in two-dimensional reconstructions of CBCT and presence of C-shape. METHODS: Mandibular jaw CBCT scans (184 males and 220 females, aged 15 to 80 years), which presented 1464 mandibular molars (710 first molars and 754 second molars), were assessed. Teeth were evaluated for the presence and type of C-shaped root canals by observing the roots at five levels in CBCT axial reconstructions. Root canal configuration was assessed in panoramic reconstructions. Data were statistically analyzed at a significance level of 5%. RESULTS: Of the 1464 mandibular molars, 125 (8.5%) were classified as C-shaped. This variation was more prevalent in females (n = 107, 85.6%) and in second molars (n = 108, 86.4%). C1 (uninterrupted C-shaped canal) was the most prevalent type of C-shaped configuration (41.76%), while C5 (no canal lumen) was the least prevalent type (0.96%). Single root with single canal in panoramic reconstructions was the most predominant configuration for C-shaped teeth (n = 54, 43.2%). Fused roots presented 17.2 higher odds of being associated with C-shaped root canals than non-fused roots. CONCLUSIONS: C-shaped root canals were more prevalent in mandibular second molars and in females. Additionally, clinicians should bear in mind the greater possibility of C-shaped configuration in mandibular molars with fused roots. CLINICAL RELEVANCE: Mandibular molars with C-shaped canals present a clinical challenge. A higher C-shaped proportion was noted in radiographic fused root types, which had 17.2 higher odds of presenting such anatomy when compared to radiographic non-fused roots. Root radiographic features may help in diagnosis of complex C-shaped morphologies.

15.
Rev. bras. odontol ; 77(1): 1-5, jan. 2020. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1117693

RESUMO

Objetivo: este estudo teve como objetivo comparar a resistência de união à dentina de um material à base de silicato de cálcio fotopolimerizável modificado por resina (TheraCal LC®; Bisco, Schaumburg, IL, EUA) com MTA branco (WMTA®; Angelus, Londrina, PR, Brasil). Materiais e Métodos: dezesseis incisivos superiores e caninos humanos foram selecionados e três discos de 1 mm foram obtidos a partir do terço médio de cada raiz. Na superfície coronal de cada disco, dois furos de 1,2 mm de largura foram perfurados na dentina. Em seguida, os buracos artificiais foram preenchidos com um dos materiais testados: WMTA® e TheraCal LC®. As fatias dentárias preenchidas foram armazenadas em uma solução salina tamponada com fosfato (PBS) (pH 7,2) por 7 dias a 37°C. Depois disso, a avaliação do push-out foi realizada com uma ponta do êmbolo de 1,0 mm. A carga foi aplicada a uma velocidade de 0,5 mm / min até o deslocamento do selador. Os resultados foram expressos em MPa. O teste U de Mann-Whitney foi aplicado para classificar os materiais quanto à resistência adesiva à dentina. O nível de significância foi estabelecido em = 5%. Resultados: todas as amostras apresentaram resultados de resistência de união à dentina mensuráveis e não ocorreram falhas prematuras. O TheraCal LC® demonstrou valores superiores de resistência de união à dentina quando comparado ao WMTA® (P<0,0001). Conclusões: existe uma vantagem do TheraCal LC® sobre o WMTA® no que diz respeito à resistência da união ao empurrar e, portanto, pode ser considerado um material reparador promissor e inovador


Objective: this study aimed to compare the dentin bond strength of a resin-modified light-curable calcium-silicate-based material (TheraCal LC®; Bisco, Schaumburg, IL, USA) with White MTA (WMTA®; Angelus, Londrina, PR, Brazil). Materials and Methods: sixteen human maxillary incisors and canines were selected and three 1-mm-discs were obtained from the middle third of each root. On the coronal surface of each disc, two 1.2-mm-wide-holes were drilled through the dentin. Then, artificial holes were filled with one of the tested materials: WMTA® and TheraCal LC®. The filled dental slices were stored in a phosphate-buffered saline (PBS) solution (pH 7.2) for 7 days at 37°C. After that, push-out assessment was performed with a 1.0-mm-plunger-tip. Load was applied at a crosshead speed of 0.5 mm/min until sealer displacement. The results were expressed in MPa. Mann-Whitney U test was applied to rank materials regarding dentin push-out bond strength. Significance level was set at a = 5%. Results: All specimens showed measurable results and no premature failure occurred. TheraCal LC® demonstrated superior push-out bond strength values to dentin when compared to WMTA® (P<0.0001). Conclusions: there is advantage of TheraCal LC® over WMTA® as regards to the push-out bond strength and, therefore it may be taken as a promising and innovative reparative material


Assuntos
Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Tratamento do Canal Radicular , Cimento de Silicato , Materiais Dentários , Endodontia , Cura Luminosa de Adesivos Dentários
16.
Rev. bras. odontol ; 77(1): 1-4, jan. 2020. tab
Artigo em Inglês | LILACS | ID: biblio-1102944

RESUMO

Objetivo: O presente estudo teve como objetivo avaliar o potencial citotóxico de três diferentes pastas endodônticas em cultura celular de osteoblastos humanos. Material e Métodos: As pastas endodônticas Calen PMCC (SSWhite Artigos Dentários LTDA, Rio de Janeiro, Brasil), Feapex (Fórmula e Ação, São Paulo, Brasil) e CTZ (Lenzafarm, Belo Horizonte, Brasil) foram preparadas e eluídas em meio de cultura celular durante 24 horas em estufa a 37°C e 5% de CO2. Foram realizadas quatro diluições distintas desses meios nas concentrações 1: 1, 1: 2, 1: 4 e 1: 8. Culturas de células de Osteoblastos Humanos da linhagem Saos-2 foram expostas a estas diluições durante 24 horas. O controle negativo foi realizado expondo as células ao meio de cultura sem contato com nenhuma pasta endodôntica. A citotoxicidade desses meios foi avaliada utilizando o ensaio MTT e os resultados foram transformados em porcentagens de células viáveis em relação ao grupo controle negativo. A análise estatística foi realizada com nível de significância de 5%, utilizando ANOVA seguido do teste de Tukey. Resultados: A viabilidade celular foi significativamente alterada de acordo com o material testado (p <0,05) e sua concentração (p <0,05). Em todas as concentrações testadas, a pasta Feapex apresentou maior viabilidade celular comparada aos demais materiais (p <0,05). Embora não tenha sido observada diferença estatisticamente significante entre a pasta Calen PMCC e a pasta CTZ nas concentrações de 1: 1 e 1: 2 (p> 0,05), a pasta CTZ apresentou maior citotoxicidade nas concentrações de 1: 4 e 1: 8 (p <0,05 ). De forma geral, a citotoxicidade diminuiu com o aumento da diluição do material. Conclusão: De acordo com os resultados do presente estudo, a pasta endodôntica Feapex parece ser a melhor opção para utilização entre as pastas analisadas, pois apresentou menor citotoxicidade que as pastas Calen PMCC e CTZ


Objective: The aim of the present study was to evaluate the cytotoxic potential of three different endodontic filling materials on human osteoblast cell cultures. Material and Methods: The endodontic pastes Calen PMCC (SSWhite Dental Articles LTDA, Rio de Janeiro, Brazil), Feapex (Formula and Action, São Paulo, Brazil) and CTZ (Lenzafarm, Belo Horizonte, Brazil) were eluted in cell culture medium during 24 hours in an oven at 37°C and 5% CO2. Four distinct dilutions of these media were performed at the concentrations 1:1, 1:2, 1:4 and 1:8. Cell cultures of Saos-2 Human Osteoblast-like were exposed to these dilutions for 24 hours. The negative control group was performed by exposing the cells to culture medium without contact with any endodontic paste. The cytotoxic potential of these media was evaluated using the MTT assay and the results were transformed into viable cell percentages in relation to the negative control group. Statistical analysis was submitted with a significance level of 5%, using univariate Analysis of Variance (ANOVA) followed by Tukey's test. Results: Cell viability was significantly altered according to the material tested (p<0.05) and its concentration (p<0.05). Feapex samples presented higher cell viability than the other materials in all concentrations tested (p<0.05). Although no statistically significant difference was observed between Calen PMCC paste and CTZ paste at concentrations of 1: 1 and 1: 2 (p>0.05), CTZ paste showed a higher cytotoxicity at concentrations of 1: 4 and 1: 8 (p<0.05). In general, cytotoxicity decreases with increasing material dilution. Conclusion: According to the results of the present study, Feapex endodontic paste seems to be the better option for use among the analyzed pastes, since it presented lower cytotoxicity than Calen PMCC and CTZ pastes


Assuntos
Tratamento do Canal Radicular , Odontopediatria , Endodontia , Materiais Restauradores do Canal Radicular/farmacologia
17.
Rev. bras. odontol ; 77(1): 1-5, jan. 2020. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1103880

RESUMO

Objetivo: O objetivo deste estudo foi avaliar a influência do consumo crônico de álcool no aumento da destruição óssea periapical em ratos. Material e métodos: Foram selecionados 12 ratos machos da linhagem Wistar, aleatoriamente divididos nos grupos controle e álcool (n=6). Os ratos do grupo álcool foram submetidos à auto-administração de solução alcoólica contendo 25% de álcool puro. O grupo controle recebeu apenas água filtrada durante o estudo. Após as 5 semanas de adaptação do grupo álcool, todos os ratos foram anestesiados e a polpa dos seus primeiros molares inferiores esquerdos foi exposta à cavidade oral para indução da lesão periapical. Após 28 dias de exposição pulpar, os ratos foram sacrificados por sobredose de anestesia, e suas mandíbulas foram removidas e seccionadas para avaliação microtomográfica. As hemimandíbulas esquerdas foram fixadas e escaneadas no microtomógrafo SkyScan 1173 (Bruker, Kontich, Belgium). O tamanho da lesão periradicular foi medido a partir das imagens de microtomografia computadorizada (micro-CT), onde foram calculados a área de superfície e o volume da lesão. Também foram avaliadas a taxa percentual de ganho de peso e a ingestão de sólidos/líquidos dos grupos. Os dados foram estatisticamente analisados utilizando o teste t de Student (p<0,05). Resultados: Os animais do grupo controle tiveram uma maior taxa percentual de ganho de peso corporal e de ingestão tanto de sólidos como de líquidos (p<0,05). As lesões periapicais apresentaram maior volume e maior área nos animais do grupo álcool, em comparação ao grupo controle (p<0,05). Conclusão: O consumo crônico de álcool contribuiu para o aumento da destruição óssea periapical em casos de periodontite apical.


Objective: the aim of this study was to evaluate the influence of alcohol consumption on the increase of periapical bone destruction in rats. Material and Methods: the sample included 12 Wistar male rats, randomly assigned into a control group and an alcohol group (n=6). Rats in the alcohol group were submitted to self-administration of a 25% pure alcoholic solution. The control group received only filtered water throughout the study. After 5 weeks of adaptation to the alcohol dose, all animals were anesthetized and the pulps of their mandibular left first molar were exposed to the oral cavity to induce periapical lesion. Twenty-eight days after the pulp exposure, those rats were euthanized due to overdose of anesthesia and their mandibles were removed and sectioned to obtain a micro-computed tomographic (micro-CT) scan. The rats' left hemimandibles were fixed and scanned on the SkyScan 1173 (Bruker, Konitch, Belgium) microtomograph. The size of the periradicular lesions was measured from the images obtained on the micro-CT and the surface area and volume were calculated. It was also evaluated the weight gain rate and the ingestion of solid/liquid of both groups. Data were analyzed by the Student's t-test (p<0.05). Results: the control group showed higher rates of weight gain and ingested more solid and liquid than the alcohol group (p<0.05). Periapical lesions found in the alcohol group had higher volume and surface area than the ones of the control group (p<0.05). Conclusion: the chronic consumption of alcohol contributed to the increase of periapical bone destruction in cases of apical periodontitis


Assuntos
Periodontite , Alcoolismo , Microtomografia por Raio-X
18.
Aust Endod J ; 46(2): 191-196, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31814249

RESUMO

This study evaluated the antimicrobial effectiveness of 6.5% Vitis vinifera grape seed extract (GSE) against Enterococcus faecalis biofilm using confocal laser scanning microscopy (CLSM). Saline solution (SS), 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) were used for comparison. Dentin discs were inoculated with E. faecalis strain establishing a 3-week-old biofilm. Discs (n = 10) were exposed to 5.25% NaOCl, 2% CHX, 6.5% GSE and SS (negative control) for 10 min. Discs were stained with the fluorescent LIVE/DEAD-BacLight™ dye and analysed using CLSM. The proportion of dead cells in biofilm was analysed using one-way anova and Tukey tests (P < 0.05). A higher proportion of dead cells was found in GSE group compared with CHX and SS (P < 0.05). NaOCl group was associated with the highest proportion of dead cells (P < 0.05). GSE presented antimicrobial activity against E. faecalis; however, NaOCl was the most effective irrigant solution. GSE was more effective than CHX and SS.


Assuntos
Enterococcus faecalis , Extrato de Sementes de Uva , Biofilmes , Clorexidina , Dentina , Microscopia Confocal , Irrigantes do Canal Radicular , Hipoclorito de Sódio
19.
Arch Oral Biol ; 113: 104589, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31735252

RESUMO

OBJECTIVE: To assess the influence of cone-beam computed tomographic (CBCT) voxel size and population demographic aspects (age, gender and geographic region) on the prevalence of the second mesiobuccal root canal (MB2) in maxillary first and second molars. DESIGN: Prevalence studies using CBCT technology on MB2 canal were searched between May and September 2019. The protocol was registered in PROSPERO. Four electronic databases and 5 peer-reviewed endodontic journals were screened. Authors were contacted and bibliographic references hand-searched. Eighty three studies were submitted to full text analysis and scientific merit evaluation by 2 evaluators using Joanna Briggs Institute Critical Appraisal tool. Twenty six studies were finally pooled into a meta-analysis. Forest plots with a 95% confidence interval were undertaken. Meta-regression was used to identify possible sources of heterogeneity and funnel plot visual analysis to assess publication bias. RESULTS: The 26 studies reported anatomical data from the mesiobuccal root of 15,285 first molars and 8,641 second molars. Pooled prevalence of MB2 canal was higher in maxillary first molar (69.6%; 64.5%-74.8%) than in second molars (39.0%; 31.1%-46.9%) (p < 0.05). A significantly higher prevalence odds of having MB2 canal was detected in males (p < 0.05). Meta-regression excluded gender, age and voxel size as a source variance, but identified tooth group and geographic region as possible sources of heterogeneity. CONCLUSION: MB2 canal prevalence was significantly higher in maxillary first molars. Males showed higher odds of having MB2 canal than females. Geographic region seemed to influence MB2 prevalence outcome. Knowing these preoperative factors would help to anticipate MB2 morphologies in clinics.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Cavidade Pulpar/diagnóstico por imagem , Dente Molar , Estudos Transversais , Feminino , Humanos , Masculino , Maxila , Raiz Dentária
20.
Braz. oral res. (Online) ; 34: e069, 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1132696

RESUMO

Abstract The aim of this randomized clinical trial was to compare the occurrence and intensity of postoperative pain and analgesic intake after root canal treatment, using different root canal sealers. Sixty single-rooted teeth diagnosed with asymptomatic necrosis and apical periodontitis were randomly assigned to 3 experimental groups (n=20), according to the root canal sealer: AH Plus, Endofill or MTA Fillapex. Endodontic treatment was performed in two sessions, and calcium hydroxide was used as the intracanal dressing. Patients were instructed to record pain intensity as none, slight, moderate and severe. Scores from 1 to 4 were attributed to each level of pain after 24 h, 48 h and 7 days. The need for analgesic intake was also recorded. Differences in the incidence of postoperative pain and the need for an analgesic were analyzed using the chi-square test. Differences in pain intensity after treatment were analyzed using the ordinal (linear) chi-square test. No significant differences were detected among the groups in terms of either incidence or intensity of postoperative pain, or need for analgesic intake, at any timepoint (p>0.05). No pain was reported after 7 days. AH Plus, Endofill and MTA Fillapex used for filling root canals resulted in the same rate of postoperative pain and need for analgesic medication.

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