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1.
Emerg Microbes Infect ; 8(1): 218-231, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30866775

RESUMO

The yellow fever virus (YFV) caused a severe outbreak in Brazil in 2016-2018 that rapidly spread across the Atlantic Forest in its most populated region without viral circulation for almost 80 years. A comprehensive entomological survey combining analysis of distribution, abundance and YFV natural infection in mosquitoes captured before and during the outbreak was conducted in 44 municipalities of five Brazilian states. In total, 17,662 mosquitoes of 89 species were collected. Before evidence of virus circulation, mosquitoes were tested negative but traditional vectors were alarmingly detected in 82% of municipalities, revealing high receptivity to sylvatic transmission. During the outbreak, five species were found positive in 42% of municipalities. Haemagogus janthinomys and Hg. leucocelaenus are considered the primary vectors due to their large distribution combined with high abundance and natural infection rates, concurring together for the rapid spread and severity of this outbreak. Aedes taeniorhynchus was found infected for the first time, but like Sabethes chloropterus and Aedes scapularis, it appears to have a potential local or secondary role because of their low abundance, distribution and infection rates. There was no evidence of YFV transmission by Aedes albopictus and Aedes aegypti, although the former was the most widespread species across affected municipalities, presenting an important overlap between the niches of the sylvatic vectors and the anthropic ones. The definition of receptive areas, expansion of vaccination in the most affected age group and exposed populations and the adoption of universal vaccination to the entire Brazilian population need to be urgently implemented.


Assuntos
Surtos de Doenças , Mosquitos Vetores/classificação , Febre Amarela/epidemiologia , Febre Amarela/transmissão , Animais , Brasil/epidemiologia , Cidades , Feminino , Masculino , Mosquitos Vetores/virologia , Filogeografia , Dinâmica Populacional , Vírus da Febre Amarela
2.
Einstein (Sao Paulo) ; 16(3): eAO4206, 2018 Sep 17.
Artigo em Inglês, Português | MEDLINE | ID: mdl-30231143

RESUMO

OBJECTIVE: To evaluate the effects of right sciatic nerve compression and cryotherapy on muscle tissue. METHODS: We used 42 male Wistar rats, subdivided in the following Groups Control, Injury 3, Injury 8 and Injury 15 submitted to nerve compression and euthanized in the 3rd, 8th and 15th day after surgery. The Cryotherapy Injury 3 was entailed treatment with cryotherapy by immersion of the animal in recipient for 20 minutes during 1 day, then animals were euthanized at the 3rd day after surgery, and the Cryotherapy Injury 8 and the Cryotherapy Injury 15 was treated for 6 days, and euthanized at the 8th and 15th day after surgery. Functional evaluation was performed by the grasping strength of the right pelvic limb. The right tibialis anterior muscles were evaluated for mass, smaller diameter and cross-sectional area. In the Cryotherapy Injury 8 and the Cryotherapy Injury 15 groups, the hydroxyproline was dosed in the right soles. RESULTS: In the compression there was a significant difference in the Injury Groups compared with the Control Group (p<0.05). In the smaller diameter, the compression in Control Group was higher than Injury 8 (p=0.0094), Injury 15 (p=0.002) and Cryotherapy Injury 15 (p<0.001) groups. The comparison between groups with euthanasia in the same post-operative period, a significant difference (p=0.0363) was seen in day 8th after surgery, and this result in Cryotherapy Injury Group was greater than Injury Group. In the fiber area, Control Group was also higher than the Injury 8 (p=0.0018), the Injury 15 (p<0.001) and the Cryotherapy Injury 15 (p<0.001). In hydroxyproline, no significant difference was seen between groups. CONCLUSION: Nerve damage resulted in decreased muscle strength and trophism, the cryotherapy delayed hypotrophy, but this effect did not persist after cessation of treatment.


Assuntos
Crioterapia/métodos , Síndromes de Compressão Nervosa/patologia , Síndromes de Compressão Nervosa/terapia , Nervo Isquiático/patologia , Neuropatia Ciática/patologia , Neuropatia Ciática/terapia , Animais , Modelos Animais de Doenças , Hipertrofia/fisiopatologia , Masculino , Debilidade Muscular/fisiopatologia , Síndromes de Compressão Nervosa/fisiopatologia , Distribuição Aleatória , Ratos Wistar , Valores de Referência , Reprodutibilidade dos Testes , Nervo Isquiático/fisiopatologia , Nervo Isquiático/cirurgia , Neuropatia Ciática/fisiopatologia , Fatores de Tempo , Resultado do Tratamento
3.
Rev Bras Ortop ; 53(3): 276-280, 2018 May-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29892576

RESUMO

Objective: To evaluate the neurotrophin mRNA expression and axon count in the median nerve of Wistar rats submitted to neural mobilization (NM) after nerve compression. Methods: Eighteen animals were randomly divided into G1 (nerve compression only), G2 (NM for 1 min), and G3 (NM for 3 min). For NM, the animals were anesthetized and the right scapula received the mobilization, adapted as indicated for humans, on alternate days, from the third to the 13th postoperative (PO) day, totaling six days of therapy. On the 14th PO day, animals were anesthetized and euthanized. Fragments of the median nerve, distal to the compression procedure, were removed for histomorphometric analysis and expression of neurotrophins, nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF) by RT-PCR. Results: Histomorphometric analysis revealed differences in the number of axons in the injured side, which was significantly lower in the injured limb nerve compared to the control limb, whereas the RT-PCR analysis showed no significant differences in the expression of NGF or BDNF. Conclusion: NM treatment did not affect median nerve regeneration, which maintained normal recovery rates.

4.
Rev. bras. ortop ; 53(3): 276-280, May-June 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-959146

RESUMO

ABSTRACT Objective: To evaluate the neurotrophin mRNA expression and axon count in the median nerve of Wistar rats submitted to neural mobilization (NM) after nerve compression. Methods: Eighteen animals were randomly divided into G1 (nerve compression only), G2 (NM for 1 min), and G3 (NM for 3 min). For NM, the animals were anesthetized and the right scapula received the mobilization, adapted as indicated for humans, on alternate days, from the third to the 13th postoperative (PO) day, totaling six days of therapy. On the 14th PO day, animals were anesthetized and euthanized. Fragments of the median nerve, distal to the compression procedure, were removed for histomorphometric analysis and expression of neurotrophins, nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF) by RT-PCR. Results: Histomorphometric analysis revealed differences in the number of axons in the injured side, which was significantly lower in the injured limb nerve compared to the control limb, whereas the RT-PCR analysis showed no significant differences in the expression of NGF or BDNF. Conclusion: NM treatment did not affect median nerve regeneration, which maintained normal recovery rates.


RESUMO Objetivo: Avaliar a expressão de RNAm de neurotrofinas e a contagem de axônios no nervo mediano de ratos Wistar submetidos à mobilização neural (MN) após compressão nervosa. Métodos: Foram divididos aleatoriamente 18 animais em G1 (apenas compressão nervosa), G2 (MN por 1 minuto) e G3 (MN por 3 minutos). Para a MN, os animais foram anestesiados e o membro escapular direito recebeu a mobilização, adaptada da forma indicada para humanos, em dias alternados, do terceiro ao 13° dia de pós-operatório (PO), em seis dias de terapia. No 14° dia PO, os animais foram anestesiados e eutanasiados. Fragmentos do nervo mediano, distais ao procedimento de compressão, foram retirados para análise histomorfométrica e de expressão das neutrotrofinas, fator de crescimento do nervo (NGF) e fator de crescimento derivado do cérebro (BNDF) por RT-PCR. Resultados: A análise histomorfométrica evidenciou diferenças no número de axônios nos lados lesionados, que foi significativamente menor no nervo do membro lesado comparado com o membro controle; por sua vez, a análise por RT-PCR não apontou diferenças significativas na expressão de NGF e nem de BNDF. Conclusão: O tratamento de MN não afetou a regeneração do nervo mediano, que manteve índices normais de recuperação.


Assuntos
Animais , Ratos , Exercício , Ratos Wistar , Peptídeos e Proteínas de Sinalização Intercelular , Histologia , Nervo Mediano , Regeneração Nervosa
5.
Einstein (Säo Paulo) ; 16(3): eAO4206, 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-953187

RESUMO

ABSTRACT Objective: To evaluate the effects of right sciatic nerve compression and cryotherapy on muscle tissue. Methods: We used 42 male Wistar rats, subdivided in the following Groups Control, Injury 3, Injury 8 and Injury 15 submitted to nerve compression and euthanized in the 3rd, 8th and 15th day after surgery. The Cryotherapy Injury 3 was entailed treatment with cryotherapy by immersion of the animal in recipient for 20 minutes during 1 day, then animals were euthanized at the 3rd day after surgery, and the Cryotherapy Injury 8 and the Cryotherapy Injury 15 was treated for 6 days, and euthanized at the 8th and 15th day after surgery. Functional evaluation was performed by the grasping strength of the right pelvic limb. The right tibialis anterior muscles were evaluated for mass, smaller diameter and cross-sectional area. In the Cryotherapy Injury 8 and the Cryotherapy Injury 15 groups, the hydroxyproline was dosed in the right soles. Results: In the compression there was a significant difference in the Injury Groups compared with the Control Group (p<0.05). In the smaller diameter, the compression in Control Group was higher than Injury 8 (p=0.0094), Injury 15 (p=0.002) and Cryotherapy Injury 15 (p<0.001) groups. The comparison between groups with euthanasia in the same post-operative period, a significant difference (p=0.0363) was seen in day 8th after surgery, and this result in Cryotherapy Injury Group was greater than Injury Group. In the fiber area, Control Group was also higher than the Injury 8 (p=0.0018), the Injury 15 (p<0.001) and the Cryotherapy Injury 15 (p<0.001). In hydroxyproline, no significant difference was seen between groups. Conclusion: Nerve damage resulted in decreased muscle strength and trophism, the cryotherapy delayed hypotrophy, but this effect did not persist after cessation of treatment.


RESUMO Objetivo: Avaliar os efeitos da compressão nervosa do isquiático direito e da crioterapia no tecido muscular. Métodos: Foram utilizados 42 ratos Wistar machos, subdivididos nos Grupos Controle, Lesão 3, Lesão 8 e Lesão 15, submetidos a compressão nervosa e eutanasiados, respectivamente, no 3°, 8° e 15° dias pós-operatório; Lesão Crioterapia 3, tratado com crioterapia, por imersão durante 20 minutos, por 1 dia, e eutanasiados no 3° dia pós-operatório; e Lesão Crioterapia 8 e Lesão Crioterapia 15, tratados durante 6 dias e eutanasiados no 8° e 15° dias pós-operatório. A avaliação funcional foi realizada pela força de preensão do membro pélvico direito. Os músculos tibiais anteriores direitos foram avaliados quanto a massa, menor diâmetro e área de secção transversa. Em Lesão Crioterapia 8 e Lesão Crioterapia 15, foi dosada a hidroxiprolina nos sóleos direitos. Resultados: Na preensão, houve diferença significativa nos Grupos Lesão quando comparados ao Grupo Controle (p<0,05). No menor diâmetro, o Grupo Controle foi maior que Lesão 8 (p=0,0094), Lesão 15 (p = 0,002) e Lesão Crioterapia 15 (p<0,001). Na comparação entre os grupos com eutanásia no mesmo pós-operatório, houve diferença significativa (p=0,0363) no 8° pós-operatório, sendo Lesão Crioterapia maior que Lesão. Na área das fibras, o Grupo Controle também foi maior que Lesão 8 (p=0,0018), Lesão 15 (p<0,001) e Lesão Crioterapia 15 (p<0,001). Na hidroxiprolina, não houve diferença significativa entre os grupos. Conclusão: A lesão nervosa resultou na diminuição da força e em trofismo muscular, e a crioterapia retardou a hipotrofia, porém este efeito não se manteve após o tratamento cessar.

6.
Motriz (Online) ; 23(4): e1017100, 2017. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-895023

RESUMO

AIMS: Platelet-Rich Fibrin (PRF) is a new and promising technique for tissue repair, however, there is still a gap about its action on peripheral nerve injury, as well as its association with physical exercise. Thus, the aim of this study was to evaluate the effects of the PRF associated with physical exercise in a model of median nerve compression. METHODS: 42 rats, divided into: Control, non-injured limb, and other six groups with nerve lesion (Control lesion; Treated with PRF; Treadmill walking; Free swimming; PRF and treadmill; PRF and swimming). The lesion model was performed with median nerve compression. To obtain the PRF, 1.5 mL of blood was centrifuged and the fibrin clot positioned directly over the compression region. The exercise protocols were performed during 2 weeks. The evaluations performed were grip strength tests and histologic analysis. RESULTS: both, grip strength and histomorphometric evaluations (fiber numbers and axon density) did not present significant differences between the treated and lesion groups, however, in the morphological evaluation it was possible to distinguish characteristics of the repair process for the treated groups. CONCLUSION: a slight qualitative improvement was observed for the treated groups, especially when it was associated PRF with free swimming.(AU)


Assuntos
Animais , Masculino , Ratos , Terapia por Exercício , Fibrina , Nervo Mediano , Força Muscular , Ratos Wistar
7.
Braz. j. microbiol ; 47(3): 680-690, July-Sept. 2016. tab, graf
Artigo em Inglês | LILACS-Express | ID: lil-788964

RESUMO

ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of β-xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the β-xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production.

8.
Rev. dor ; 17(2): 121-124, tab
Artigo em Português | LILACS-Express | ID: lil-787999

RESUMO

RESUMO JUSTIFICATIVA E OBJETIVOS: Fibrina rica em plaquetas é uma técnica nova e promissora na aceleração do reparo, com possíveis efeitos analgésicos, contudo, ainda há uma lacuna com relação à lesão nervosa periférica, bem como com a associação com exercícios físicos. Assim, o objetivo deste estudo foi avaliar os efeitos da fibrina rica em plaquetas associada a exercício físico sobre a nocicepção e o edema, em modelo experimental de compressão do nervo mediano. MÉTODOS: Foram utilizados 36 ratos, todos submetidos a compressão do nervo mediano e divididos em seis grupos: G1: sem manipulações adicionais; G2: compressão e tratado com fibrina rica em plaquetas; G3: compressão e tratado com natação livre; G4: compressão e exercício de caminhada em esteira; G5: natação livre + fibrina rica em plaquetas; G6: caminhada em esteira + fibrina rica em plaquetas. O modelo de lesão foi realizado com amarria do nervo mediano, com fio catgut 4.0 cromado. Para obtenção da fibrina rica em plaquetas, 1,5mL de sangue foi centrifugado e o coágulo de fibrina foi posicionado diretamente sobre a região da compressão. Os protocolos de exercício foram realizados durante 2 semanas, entre o 3º e 14º dias de pós-operatório. As avaliações nociceptivas e de edema ocorreram, respectivamente, pelo limiar de retirada de pata e pletismometria, nos momentos prévios à lesão, no 3º, 7º e 15º dias de pós-operatório. RESULTADOS: Não houve diferenças entre os grupos, apenas entre as avaliações, denotando que houve aumento da nocicepção e do edema, o qual perdurou ou foi decaindo, respectivamente, com o passar do tempo. CONCLUSÃO: O uso isolado ou associado da fibrina rica em plaquetas com exercícios físicos não produziu alterações na nocicepção e edema.


ABSTRACT BACKGROUND AND OBJECTIVES: Platelet-rich fibrin is a new and promising technique to accelerate repair, with possible analgesic effects; however, there is still a gap with regard to peripheral nerve injury and the association with physical exercises. So, this study aimed at evaluating the effects of platelet-rich fibrin associated to physical exercises on nociception and edema in experimental median nerve compression model. METHODS: Thirty-six rats, all submitted to median nerve compression, were divided in six groups: G1: without additional manipulation; G2: compression and treated with platelet-rich fibrin; G3: compression and treated with free swimming; G4: compression and walking on a treadmill; G5: free swimming + platelet-rich fibrin; G6: walking on a treadmill + platelet-rich fibrin. Injury was induced by tying the median nerve with chrome plated catgut 4.0. Platelet-rich fibrin was obtained by centrifuging 1.5 mL of blood and positioning the fibrin clot directly on the compression region. Exercises were carried out during two weeks, between the 3rd and 14th postoperative days. Nociception and edema were evaluated, respectively, by flinch threshold and plethysmometer, in moments before injury and in the 3rd, 7th and 15th postoperative days. RESULTS: There have been no differences among groups, only among evaluations, showing increased nociception and edema, which has lasted or improved, respectively, over time. CONCLUSION: Platelet-rich fibrin alone or associated to physical exercises has not changed nociception and edema.

9.
World J Microbiol Biotechnol ; 28(9): 2879-88, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22806729

RESUMO

The xynB1 gene (CCNA 01040) of Caulobacter crescentus that encodes a bifunctional enzyme containing the conserved ß-Xylosidase and α-L-Arabinofuranosidase (ß-Xyl I-α-L-Ara) domains was amplified by PCR and cloned into the vector pJet1.2Blunt. The xynB1 gene was subcloned into the vector pPROEX-hta that produces a histidine-fused translation product. The overexpression of recombinant ß-Xyl I-α-L-Ara was induced with IPTG in BL21 (DE3) and the resulting intracellular protein was purified with pre-packaged nickel-Sepharose columns. The recombinant ß-Xyl I-α-L-Ara exhibited a specific ß-Xylosidase I activity of 1.25 U mg(-1) to oNPX and a specific α-L-Arabinofuranosidase activity of 0.47 U mg(-1) to pNPA. The predominant activity of the recombinant enzyme was its ß-Xylosidase I activity, and the enzymatic characterization was focused on it. The ß-Xylosidase I activity was high over the pH range 3-10, with maximal activity at pH 6. The enzyme activity was optimal at 45 °C, and a high degree of stability was verified over 240 min at this temperature. Moreover, ß-Xylosidase activity was inhibited in the presence of the metals Zn(2+) and Cu(2+), and the enzyme exhibited K(M) and V(Max) values of 2.89 ± 0.13 mM and 1.4 ± 0.04 µM min(-1) to oNPX, respectively. The modeled structure of ß-xylosidase I showed that its active site is highly conserved compared with other structures of the GH43 family. The increase in the number of contact residues responsible for maintaining the dimeric structure indicates that this dimer is more stable than the tetramer form.


Assuntos
Caulobacter crescentus/enzimologia , Caulobacter crescentus/genética , Xilosidases/metabolismo , Sequência de Aminoácidos , Clonagem Molecular , DNA Bacteriano/genética , Genes Bacterianos , Glicosídeo Hidrolases/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Estrutura Terciária de Proteína , Recombinação Genética , Análise de Sequência de DNA , Especificidade por Substrato , Xilosidases/genética , Xilosidases/isolamento & purificação
10.
Braz. arch. biol. technol ; 51(4): 471-477, June-Aug. 2008. ilus, tab
Artigo em Inglês | LILACS-Express | ID: lil-622653

RESUMO

This study aimed at the sequence and analysis of the mtDNA control region (CR) of the Diatraea saccharalis. The genome PCR amplification was performed using the complementary primers to the flanking regions of Bombyx mori CR mitochondrial segment. The sequencing revealed that the amplified product was 568 bp long, which was smaller than that observed for B. mori (725 bp). Within the amplified segment, a sequence with 338 nucleotides was identified as the control region, which displayed a high AT content (93.5%). The D. saccharalis mtDNA CR multiple sequence alignment analysis showed that this region had high similarity with the Lepidoptera Cydia pomonella.


A broca da cana, Diatraea saccharalis pertence à família dos lepidópteros. A presença da larva pode ser extremamente destrutiva, chegando a inviabilizar a atividade canavieira, causando prejuízos consideráveis à agroindústria sucro-alcooleira. Atualmente a broca da cana vem sendo extinta da plantação por métodos de controle biológico, entretanto a evolução desses programas depende de maiores conhecimentos básicos da biologia molecular deste inseto. O estudo do segmento do genoma mitocondrial denominado região controle é amplamente utilizado em análises genéticas e filogenéticas em insetos. O objetivo desse trabalho foi sequenciar e analisar a região controle do genoma mitocondrial de Diatraea saccharalis. Esse segmento apresentou 338 nucleotídeos, menor que o observado em Bombyx mori, com conteúdo de 93,5% de A/T. As analises realizadas mostraram que Diatraea saccharalis apresenta 76% de similaridade com Cydia pomonella.

11.
BMB Rep ; 41(5): 394-9, 2008 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-18510871

RESUMO

Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and from Chinese and Japanese strains demonstrated that 51.6% of the fib-L third intron is conserved. One of these conserved segments, 41 bp long, contains the sequence CGTTATTATACATATT, which is duplicated in the B. mori Nd-s(D) mutant. In the present work, electrophoretic mobility assays and computational analyses revealed a major peak of intrinsic bent DNA within the segment that undergoes breakage in the previously-described Nd-s(D) mutation. This result suggested that this intrinsically-curved region might mediate DNA cleavage and enhance recombination events in the third intron of the Bombyx mori fib-L gene.


Assuntos
Bombyx/genética , Fibroínas/genética , Mutação , Animais , Sequência de Bases , Bombyx/metabolismo , DNA/química , DNA/genética , DNA/metabolismo , Ensaio de Desvio de Mobilidade Eletroforética , Íntrons/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Análise de Sequência de DNA
12.
Braz. arch. biol. technol ; 48(3): 351-357, May 2005. ilus
Artigo em Inglês | LILACS-Express | ID: lil-415299

RESUMO

Observações à microscopia eletrônica e estudos bioquímicos de cromossomos e núcleos sem histonas tem suportado a hipótese que o DNA de eucariotos é organizado em alças associadas com o esqueleto cromossômico ou à matriz nuclear. A observação da matriz nuclear sem a remoção do DNA, através da digestão com enzimas de restrição, apresenta uma figura em halo que representa a liberação das alças de DNA. Um protocolo para a obtenção de halos nucleares de núcleos politênicos de insetos, através da extração de proteínas usando o detergente LIS, é reportado nesse trabalho. Foram realizadas análises utilizando-se microscopia de fluorescência e microscopia de varredura confocal a laser. A extração de halos nucleares foi possível somente com o isolamento da fração nuclear em tampão sem espermina e espermidina. A obtenção de halos nucleares de núcleos politênicos de glândula salivar de Bradysia hygida contribui significativamente para o estudo da estrutura e função dessas organelas tão especiais.

13.
Rev. bras. biol ; 52(1): 1-5, fev. 1992. ilus
Artigo em Português | LILACS | ID: lil-116190

RESUMO

Os autores observaram a nível da ultra-estrutura os mucocitos presentes nas três porçöes de oviduto da coelha - istmo, ampola e infundíbulo -, com a finalidade de correlacionar os seus aspectos morfológicos aos dados histoquímicos presentes na Literatura. Os resultados indicaram um único tipo de mucocito presente no istmo e de dois tipos na ampola e no infundíbulo. No que diz respeito ao mucocito presente no istmo, foi possível fazer uma correlaçäo com os dados histoquímicos. O mesmo näo ocorreu com os outros tipos encontrados na ampola e no infundíbulo. Os autores referem ainda que a ampola - no que diz respeito a morfologia dos mucocitos - pode ser considerada uma regiäo de transiçäo entre o istmo e o infundíbulo


Assuntos
Animais , Feminino , Coelhos , Tubas Uterinas/ultraestrutura , Coelhos/anatomia & histologia
14.
Rev. bras. biol ; 51(1): 221-4, fev. 1991. ilus, tab
Artigo em Português | LILACS | ID: lil-113700

RESUMO

Procurou-se detectar, por intermédio de métodos histoquímicos a natureza carboidrática do muco elaborado pelas células secretoras do epitélio que reveste as porçöes do infundíbulo, ampola e istmo do oviduto de coelha em anestro. Com base nos resultados obtidos, foi possível concluir que o oviduto de coelha é revestido por uma camada de muco. Este muco está representado por uma fraçäo neutra e outra ácida. Com relaçäo ao componente ácido, encontramos que as três porçöes do oviduto estäo revestidas por carboximucina, sendo que no infundíbulo encontramos também uma sialomucina e na ampola e no istmo uma sulfomucina. Os autores procuram relacionar estes dados com aspectos fisiológicos


Assuntos
Animais , Coelhos , Epitélio/química , Tubas Uterinas/química , Histocitoquímica/métodos , Mucinas/química , Coelhos
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