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1.
Medicine (Baltimore) ; 99(1): e18489, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895782

RESUMO

BACKGROUND: In a multitude of previous studies, Chlamydia trachomatis (CT) plays an important role in the occurrence of ectopic pregnancy (EP). However, the predictive value of CT infections in the occurrence of EP has not been estimated worldwide. We thus evaluated, by means of a meta-analysis, the current status of the association between CT infections with EP and the potential predictive value of CT infections in EP. METHODS: We evaluated studies performed between the database construction time and August 2018 published in PubMed, the Cochrane Library, EMBASE, and the Web of Science (SCI). The relationship between CT and EP was calculated based upon the predetermined entry criteria for control group selection and the original data. The related articles were analyzed using a random-effects model, and the heterogeneity of the studies was assessed using the I index. Data were analyzed with the STATA 12.0 software. RESULTS: Twenty-five studies that recruited 11960 patients were included in the present meta-analysis, and the relation of CT infections with EP were assessed. The association between CT infections and EP risk showed an odds ratio (OR) of 3.03, with a 95% confidence interval (CI) of 2.37 to 3.89. Our results showed that there was a statistically significant difference between the intervention and control groups. The prevalence of CT infections in EP was then calculated by a subgroup analysis: African (OR, 2.22; 95% CI, 1.14-4.31), European (OR, 3.16; 95% CI, 2.10-4.47), North American (OR, 3.07; 95% CI, 1.78-5.31), and Asian (OR, 3.39; 95% CI, 1.95-5.90). CONCLUSIONS: From the results of numerous studies conducted on different continents, this meta-analysis showed a clear association between EP and prior CT infections, that is, CT infections increase the risk of EP occurrence.


Assuntos
Infecções por Chlamydia/epidemiologia , Gravidez Ectópica/epidemiologia , Adulto , Chlamydia trachomatis , Feminino , Humanos , Razão de Chances , Gravidez , Gravidez Ectópica/etiologia , Prevalência , Fatores de Risco , Sensibilidade e Especificidade
2.
Medicine (Baltimore) ; 98(38): e17329, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31568019

RESUMO

Thus far, all clinical trials evaluating the efficacy of embryo transfer strategies have selectively delayed the first frozen embryo transfer (FET) by at least 1 menstrual cycle. Nevertheless, this approach, which is based solely on clinical experience, may create unnecessary psychological stress on infertile patients who are anxious to conceive as soon as possible. This study aimed to investigate whether the time interval between oocyte retrieval and subsequent FET affects reproductive outcomes.We implemented a large retrospective cohort study in a single assisted reproductive technology (ART) unit at a university-based hospital, including 1540 autologous FET cycles performed in freeze-all cycles. The beginning of the FET was classified as either 'cycle 1' (performing FET within the first menstrual cycle) or 'cycle ≥2' (performing FET after one or more menstrual cycles). Live birth rate (LBR) was the primary outcome of our study.The mean interval for 'cycle 1' and 'cycle ≥2' FETs was 25.72 ±â€Š5.10 days and 75.33 ±â€Š24.85 days, respectively (P < .001). The type of controlled ovarian hyperstimulation (COH) and endometrial preparation protocols differed significantly between groups (P = .008 and P = .004, respectively). However, FET groups were similar in many ways. Univariate analysis showed that there was no significant difference in LBR between the different cycles (33.1% after 'cycle 1' FET vs 34.2% after 'cycle ≥2' FET, P = .68). To evaluate whether LBR remained unchanged after adjustment for potential confounders, we performed multivariate logistic regression. FET timing had no significant impact on LBR in the first FET (odds ratio [OR]: 1.06, 95% confidence interval [CI]: 0.80-1.39).In accordance with the present study, it might not be necessary for clinicians to wait more than 1 menstrual cycle before performing FET. This allows us to reduce otiose deferment in FET, without adversely affecting reproductive outcomes.


Assuntos
Fase de Clivagem do Zigoto , Criopreservação , Transferência Embrionária/métodos , Ciclo Menstrual , Adulto , Feminino , Humanos , Gravidez , Resultado da Gravidez , Técnicas de Reprodução Assistida , Estudos Retrospectivos
3.
Libyan J Med ; 14(1): 1652058, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31405338

RESUMO

Objective: To investigate the effect of the duration of gonadotropin releasing hormone agonist (GnRH-a) use on the outcome of in vitro fertilization and embryo transfer (IVF-ET) during the short-acting long-term hyperstimulation cycle. Methodology: Clinical data from 776 patients receiving controlled ovarian stimulation (COS) after short-term regimen downregulation were retrospectively analyzed. According to the duration of GnRH-a, the patients were divided into 3 groups: Group A, 14 days for GnRH-a; Group B, 15-17 days for GnRH-a; and Group C, >18 days for GnRH-a. The clinical data, treatment and clinical outcomes were compared among the groups. Results: There were no significant differences in fertilization rate, implantation rate, clinical pregnancy rate, abortion rate, ovarian hyperstimulation syndrome (OHSS) rate(P > 0.05). The total costs in group A were significantly less than those in group B and C(P < 0.001). The number of eggs and quality embryos generated in group A was significantly higher than that in groups B and C (P = 0.014, P = 0.005). Conclusions: In the short-acting GnRH agonist long protocol, satisfactory IVF-ET pregnancy outcome was obtained with the use of GnRH-a for 14 days under the premise of lowering the receptor-regulating standard. Excessive application of GnRH-a will affect the number of eggs and embryos and increase the cost of medical treatment.

4.
Sci Rep ; 9(1): 10873, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31350457

RESUMO

The complexity of follicular fluid metabolome presents a significant challenge for qualitative and quantitative metabolite profiling, and for discovering the comprehensive biomarkers. In order to address this challenge, a novel SWATHtoMRM metabolomics method was used for providing broad coverage and excellent quantitative capability to discover the human follicular fluid metabolites related to recurrent spontaneous abortion (RSA) after in vitro fertilization and embryo transfer, and to evaluate their relationship with pregnancy outcome. The follicular fluid samples from the spontaneous abortion group (n = 22) and the control group (n = 22) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were used to find and validate the differential metabolites between the two groups. A total of 18 follicular fluid metabolites, including amino acids, cholesterol, vitamins, fatty acids, cholic acid, lysophosphatidylcholine and other metabolites, were identified. In the RSA group, 8 metabolites, namely dehydroepiandrosterone, lysoPC(16:0), lysoPC(18:2), lysoPC(18:1), lysoPC(18:0), lysoPC(20:5), lysoPC(20:4), and lysoPC(20:3), were up-regulated, and 10 metabolites, namely phenylalanine, linoleate, oleic acid, docosahexaenoic acid, lithocholic acid, 25-hydroxyvitamin D3, hydroxycholesterol, 13-hydroxy-alpha-tocopherol, leucine, and tryptophan, were down-regulated. These differential metabolites related to RSA may provide a possible diagnostic basis and therapeutic target for RSA, as well as a scientific basis for elucidating the mechanism of RSA.

5.
Medicine (Baltimore) ; 98(27): e16213, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31277129

RESUMO

RATIONALE: The borderline form of empty follicle syndrome (EFS) is a phenomenon where only a few mature or immature oocytes are retrieved despite adequate response to controlled ovarian hyperstimulation (COH). It is a rare phenomenon with an unclear underlying mechanism, and there is currently no effective treatment. PATIENT CONCERNS: The patient received 3 assisted reproductive technology cycles, and although her follicular development and estrogen levels were normal during COH, the outcome with respect to the oocytes obtained was unsatisfactory. DIAGNOSES: Borderline form of EFS. INTERVENTIONS: In the context of undergoing GnRH-antagonist protocol, we implemented a double-trigger with human chorionic gonadotropin (hCG) after 6 hours of gonadotropin-releasing hormone agonist (GnRH-a) administration. OUTCOMES: Eleven oocytes were obtained (M I × 3, M II × 8), which underwent in vitro fertilization (IVF). After 18 hours, 7 oocytes showed normal fertilization, with 2 embryos formed 72 hours later (embryo rating, 6C II × 1, 9C II × 1); the embryos were then frozen. LESSONS: Oocyte maturation and ovulation are time-dependent processes, and that different patients require different lengths/intervals of time for treatment. Therefore, the borderline form of EFS, in general, may be treatable, and our novel trigger method provides a new treatment option for such patients in the future.


Assuntos
Gonadotropina Coriônica/farmacologia , Fármacos para a Fertilidade Feminina/farmacologia , Hormônio Liberador de Gonadotropina/agonistas , Síndrome de Hiperestimulação Ovariana/terapia , Indução da Ovulação/métodos , Adulto , Feminino , Fertilização In Vitro , Humanos , Recuperação de Oócitos , Gravidez , Substâncias para o Controle da Reprodução/farmacologia
6.
Reprod Biol Endocrinol ; 17(1): 45, 2019 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-31186025

RESUMO

BACKGROUND: Polycystic ovary syndrome (PCOS) is a complex disorder associated with multiple metabolic disturbance, including defective glucose metabolism and insulin resistance. The altered metabolites caused by the related metabolic disturbance may affect ovarian follicles, which can be reflected in follicular fluid composition. The aim of this study is to investigate follicular fluid metabolic profiles in women with PCOS using an advanced sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry. MATERIALS AND METHODS: Nineteen women with PCOS and twenty-one healthy controls undergoing IVF/ET were recruited, and their follicular fluid samples were collected for metabolomic study. Follicular fluid metabolic profiles, including steroid hormones, free fatty acids, bioactive lipids, and amino acids were analyzed using the principal component analysis (PCA) and partial least squares to latent structure-discriminant analysis (PLS-DA) model. RESULTS: Levels of free fatty acids, 3-hydroxynonanoyl carnitine and eicosapentaenoic acid were significantly increased (P < 0.05), whereas those of bioactive lipids, lysophosphatidylcholines (LysoPC) (16:0), phytosphingosine, LysoPC (14:0) and LysoPC (18:0) were significantly decreased in women with PCOS (P < 0.05). Additionally, levels of steroid hormone deoxycorticosterone and two amino acids, phenylalanine and leucine were higher in the PCOS patients (P < 0.05). CONCLUSION: Women with PCOS display unique metabolic profiles in their follicular fluid, and this data may provide us with important biochemical information and metabolic signatures that enable a better understanding of the pathogenesis of PCOS.


Assuntos
Biomarcadores/análise , Líquido Folicular/química , Espectrometria de Massas/métodos , Metabolômica/métodos , Folículo Ovariano/química , Síndrome do Ovário Policístico/diagnóstico , Adulto , Aminoácidos/análise , Ácidos Graxos não Esterificados/análise , Feminino , Humanos , Análise dos Mínimos Quadrados , Lipídeos/análise , Síndrome do Ovário Policístico/metabolismo , Análise de Componente Principal
7.
J Pak Med Assoc ; 68(12): 1769-1781, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30504940

RESUMO

OBJECTIVE: To assess the difference in the metabolomics profiles of follicular fluid between older and younger reproductive-aged women. METHODS: The retrospective study was conducted at the Centre of Reproduction and Genetics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China and comprised patient data related to the period between July and October 2015. Follicular fluid was obtained from male-factor infertility women aged 28-35 years as the younger group A, and those aged 35-42 years as the older group B. The subjects were undergoing in vitro fertilization / intracytoplasmic sperm injection and were retrospectively analysed by ultra-performance liquid chromatography-high-resolution mass spectrometry. The fragments were structurally identified using debris' information obtained from fragmented ion scans to identify the different compounds. RESULTS: Of the 55 cases studied, 28(51%) were in group A with a mean age of 29.57 ± 2.92 years, and 27(49%) were in group B with a mean age of 39.19±2.95 years. Compared with the group A, four types of compounds, hormones, licithin, lysophospholipids, and protein degradation fragments, were expressed significantly differentially in group B (p<0.05 each). Nicotine glucuronide and phosphatidylcholine were found only in Group B follicular fluid. CONCLUSIONS: The components of follicular fluid and relative contents were found changed with ageing.


Assuntos
Envelhecimento/metabolismo , Líquido Folicular , Metaboloma/fisiologia , Metabolômica/métodos , Reprodução/fisiologia , Adulto , Biomarcadores/análise , Biomarcadores/metabolismo , Feminino , Fertilização In Vitro , Líquido Folicular/química , Líquido Folicular/metabolismo , Humanos , Infertilidade Feminina/metabolismo , Análise dos Mínimos Quadrados , Estudos Retrospectivos
8.
Pharmazie ; 73(6): 318-323, 2018 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-29880083

RESUMO

AIM OF THE STUDY: Sequential window acquisition of all theoretical fragment-ion spectra (SWATHTM), a powerful high-resolution mass spectrometric data independent acquisition technique, was used to identify differences that relate certain metabolites to endometriosis (EMT) in follicular fluid collected from EMT patients and a control group. METHODS: A case-control study was conducted to analyze the EMT-related metabolites and the IVF clinical data of 33 subjects. Subjects were divided between the observation group (17 cases, infertility due to EMT) and the control group (16 cases, infertility due to male factor, such as obstructive azoospermia). RESULTS: Analysis revealed three metabolites including phytosphingosine, LysoPC(18:2(9Z,12Z)) and LysoPC(18:0), which were closely related to infertility associated withEMT. In the EMT group, LysoPC(18:2(9Z,12Z)) and LysoPC(18:0) were upregulated, while phytosphingosine was downregulated. CONCLUSIONS: This study employed, for the first time, the SWATHTM data acquisition mode for the metabolomics study of human follicular fluid in patients with EMT. The differential metabolite profiles of follicular fluid were identified and mapped. These differential metabolites are involved in cell proliferation and apoptosis, energy metabolism, inflammatory responses and angiogenesis. The differential metabolite profile may be a new tool for early noninvasive assessment of the developmental potential of oocytes in patients with EMT.


Assuntos
Endometriose/metabolismo , Líquido Folicular/metabolismo , Espectrometria de Massas/métodos , Metabolômica/métodos , Adulto , Estudos de Casos e Controles , Endometriose/complicações , Feminino , Fertilização In Vitro/métodos , Humanos , Infertilidade/etiologia , Infertilidade/metabolismo , Lisofosfatidilcolinas/metabolismo , Masculino , Esfingosina/análogos & derivados , Esfingosina/metabolismo
9.
Biomed Res Int ; 2018: 1780391, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29951527

RESUMO

Objective: We reveal the relationship between progesterone level in follicular fluid and oocyte quality based on sequential window acquisition of all theoretical fragment-ion spectra (SWATH™), a powerful high-resolution mass spectrometric data independent acquisition technique. Method: Follicular fluid samples were collected from 22 subjects (the level of progesterone > 1.5 ng/mL) of progesterone group, as well as from 22 subjects (the level of progesterone < 1.5 ng/mL) of control group, and analyzed using UPLC-Q-TOF. All methods were performed in accordance with ISO 9001:2008. Novel SWATH acquisition mode on an ultra-high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (with resolving power 20,000-40,000) was investigated for the analysis of human follicular fluid. The principal component variable grouping detects intersample variable correlation and groups variables with similar profiles which simplifies interpretation and highlights related ions and fragments. It can also extract product ion spectra from the data collected by fragmenting a wide precursor ion window. Results: Follicular fluid from the two groups differed with respect to five metabolites. Follicular fluid from the progesterone group contained elevated levels of 8-hydroxyguanosine and 4-hydroxynonenal and reduced levels of ATP, estradiol, and L-carnitine. The increased progesterone level on the day of HCG injection could negatively impact oocyte quality, thus reducing the pregnancy rate of IVF patients.


Assuntos
Líquido Folicular/metabolismo , Metabolômica , Oócitos , Progesterona/sangue , Cromatografia Líquida de Alta Pressão , Estradiol , Feminino , Fertilização In Vitro , Humanos , Gravidez
10.
Sensors (Basel) ; 14(11): 21791-825, 2014 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-25412217

RESUMO

This paper presents a real-time motion planning approach for autonomous vehicles with complex dynamics and state uncertainty. The approach is motivated by the motion planning problem for autonomous vehicles navigating in GPS-denied dynamic environments, which involves non-linear and/or non-holonomic vehicle dynamics, incomplete state estimates, and constraints imposed by uncertain and cluttered environments. To address the above motion planning problem, we propose an extension of the closed-loop rapid belief trees, the closed-loop random belief trees (CL-RBT), which incorporates predictions of the position estimation uncertainty, using a factored form of the covariance provided by the Kalman filter-based estimator. The proposed motion planner operates by incrementally constructing a tree of dynamically feasible trajectories using the closed-loop prediction, while selecting candidate paths with low uncertainty using efficient covariance update and propagation. The algorithm can operate in real-time, continuously providing the controller with feasible paths for execution, enabling the vehicle to account for dynamic and uncertain environments. Simulation results demonstrate that the proposed approach can generate feasible trajectories that reduce the state estimation uncertainty, while handling complex vehicle dynamics and environment constraints.

11.
Sensors (Basel) ; 11(8): 7341-63, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22164021

RESUMO

The star centroid estimation is the most important operation, which directly affects the precision of attitude determination for star sensors. This paper presents a theoretical study of the systematic error introduced by the star centroid estimation algorithm. The systematic error is analyzed through a frequency domain approach and numerical simulations. It is shown that the systematic error consists of the approximation error and truncation error which resulted from the discretization approximation and sampling window limitations, respectively. A criterion for choosing the size of the sampling window to reduce the truncation error is given in this paper. The systematic error can be evaluated as a function of the actual star centroid positions under different Gaussian widths of star intensity distribution. In order to eliminate the systematic error, a novel compensation algorithm based on the least squares support vector regression (LSSVR) with Radial Basis Function (RBF) kernel is proposed. Simulation results show that when the compensation algorithm is applied to the 5-pixel star sampling window, the accuracy of star centroid estimation is improved from 0.06 to 6 × 10(-5) pixels.

12.
Opt Express ; 16(3): 1448-59, 2008 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-18542219

RESUMO

The estimate of the scene information, such as the region of ground/non-ground, the relative depth of the ground and the unevenness of ground, is important for applications such as video surveillance, mapbuilding and etc. Previous research in this field is based on specific assumptions which are difficult to satisfy in practical situations. In this paper a practical algorithm is proposed to estimate the scene information in monocular video. With the pedestrian detection results for a period of time, the Pedestrian-Scene Map (PS Map), consisting of the average width of a pedestrian and occurrence probability of a pedestrian at each position of the scene, is learned by integrating the pedestrian samples with different sizes at different positions of the scene. Furthermore, the relative depth of ground region, the ground/non-ground region and the unevenness of ground can be measured with PS Map. Experimental results illustrated the proposed method's effectiveness with stationary uncalibrated camera for unconstrained environment.


Assuntos
Algoritmos , Inteligência Artificial , Aumento da Imagem/métodos , Interpretação de Imagem Assistida por Computador/métodos , Imagem Tridimensional/métodos , Reconhecimento Automatizado de Padrão/métodos , Gravação em Vídeo/métodos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
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