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1.
J Nanosci Nanotechnol ; 20(2): 789-801, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383074

RESUMO

Magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites were prepared via a solution combustion process, and were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), Energy dispersive X-ray Spectroscopy (EDX) and vibrating sample magnetometer (VSM). The magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites were employed to remove Congo red (CR) from aqueous solution, and the adsorption process was optimized by response surface methodology (RSM). The optimum conditions were the silica content of 12.6 wt%, the calcination temperature of 501 °C and the pH value of 7.13. The adsorption kinetics and the adsorption isotherm of CR onto magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites at room temperature were investigated, and the intraparticle diffusion kinetics model and Redlich-Peterson isotherm model fitted well the respective process.

2.
Sensors (Basel) ; 19(17)2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31470505

RESUMO

A surface plasmon resonance temperature sensor based on a side opening hollow-core microstructured optical fiber is proposed in this paper. This design employs a gold nanowire to excite the plasmon mode, and can be easily filled with the sensing medium through the side opening of the fiber, which not only simplifies the fabrication of the sensor but can also use the high refractive index sensing medium. The coupling characteristics, sensing performance and fabrication tolerance of the sensor are analyzed by using the finite element method. The simulation results indicate that the maximum sensitivity is 3.21 nm/°C for the x-polarized core mode in the temperature range of 13.27-50.99 °C, and 4.98 nm/°C for the y-polarized core mode in the temperature range of 14.55-51.19 °C, when benzene is used as the sensing medium. The sensor also shows a good stability in the range of ±10% fabrication tolerance.

3.
Metab Brain Dis ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31482248

RESUMO

Alzheimer's disease (AD), a chronic, progressive, neurodegenerative disorder, is the most common type of dementia. Beta amyloid (Aß) peptide aggregation and phosphorylated tau protein accumulation are considered as one of the causes for AD. Our previous studies have demonstrated the neuroprotective effect of the Rho kinase inhibitor fasudil, but the mechanism remains elucidated. In the present study, we examined the effects of fasudil on Aß1-42 aggregation and apoptosis and identified the intracellular signaling pathways involved in these actions in primary cultures of mouse hippocampal neurons. The results showed that fasudil increased neurite outgrowth (52.84%), decreased Aß burden (46.65%), Tau phosphorylation (96.84%), and ROCK-II expression. In addition, fasudil reversed Aß1-42-induced decreased expression of Bcl-2 and increases in caspase-3, cleaved-PARP, phospho-JNK(Thr183/Tyr185), and phospho-ASK1(Ser966). Further, fasudil decreased mitochondrial membrane potential and intracellular calcium overload in the neurons treated with Aß1-42. These results suggest that inhibition of Rho kinase by fasudil reverses Aß1-42-induced neuronal apoptosis via the ASK1/JNK signal pathway, calcium ions, and mitochondrial membrane potential. Fasudil could be a drug of choice for treatment of Alzheimer's disease.

4.
J Clin Invest ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31483291

RESUMO

Electronic nicotine delivery systems (ENDS) or e-cigarettes have emerged as a popular recreational tool among adolescents and adults. Although the use of ENDS is often promoted as a safer alternative to conventional cigarettes, few comprehensive studies have assessed the long-term effects of vaporized nicotine and its associated solvents, propylene glycol (PG) and vegetable glycerin (VG). Here, we show that compared with smoke exposure, mice receiving ENDS vapor for 4 months failed to develop pulmonary inflammation or emphysema. However, ENDS exposure, independent of nicotine, altered lung lipid homeostasis in alveolar macrophages and epithelial cells. Comprehensive lipidomic and structural analyses of the lungs revealed aberrant phospholipids in alveolar macrophages and increased surfactant-associated phospholipids in the airway. In addition to ENDS-induced lipid deposition, chronic ENDS vapor exposure downregulated innate immunity against viral pathogens in resident macrophages. Moreover, independent of nicotine, ENDS-exposed mice infected with influenza demonstrated enhanced lung inflammation and tissue damage. Together, our findings reveal that chronic e-cigarette vapor aberrantly alters the physiology of lung epithelial cells and resident immune cells and promotes poor response to infectious challenge. Notably, alterations in lipid homeostasis and immune impairment are independent of nicotine, thereby warranting more extensive investigations of the vehicle solvents used in e-cigarettes.

5.
J Phys Chem B ; 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31401833

RESUMO

The investigation on proteinlike specific functions of nanoparticles (NPs) has been a huge challenge. Here, the biocompatibility of Au nanoparticles (AuNPs) to antigens hen egg white lysozyme and epidermal growth factor receptor was studied first by molecular dynamics (MD) simulations and the research results revealed that antigens could form quickly a stable binding with the AuNPs and kept the structural integrity of the protein, which demonstrated better biocompatibility of AuNPs. Then, two types of complementary-determining regions (CDRs) were grafted onto the AuNPs to design a novel multi-CDR-functional nanobody. By means of MD simulations under physiological conditions, we found that the bindings of the designed nanobody and the antigens were stable and safe. Compared with the results of antigens interacting with the natural antibody, the redundant CDRs on AuNPs bound with the nonactive site in the antigens to form a stable conformation, which leaded to the powerful binding capacity of the designed nanobody than that of the natural antibody. This study provided available insights into the biocompatibility of AuNPs and important theoretical proofs to the multi-CDR-functional nanobody applied in biological systems, which were expected to help in design of novel multifunctional nanobodies.

7.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1131-1137, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418368

RESUMO

OBJECTIVE: To explore the clinical and pathologic features as well as prognosis of systemic EBV-positive T-cell lymphoma in children. METHODS: The clinical data including clinical manifestation, pathologic changes and treatment in 16 patients with children's systemic EBV-positive T-cell lymphoma were analyzed retrospectively, and follow-up of patients were carried out. RESULTS: The 16 cases included 12 males and 4  females with median age of 3.3 years old. It was demonstrated that the clinical and pathological features of the children's systemic EBV-positive T-cell lymphoma were as followed fever, hepatosplenomegaly, cytopenia, lymphadenopathy, and hemophagocytosis in bone marrow or organ. Histologically, the structures of lymph node was normal, partially or completely destoryed. The paracortical zone was expanded with prominent infiltration of small to medium-sized atypical lymphocytes. The major immunophenotypic characteristics were as follows: (1) Almost all biopsies exhibited prominent T cell proliferation. (2) CD3 was expressed in 16 patients (100%, 16/16), CD4 in 5 patients (31.3%, 5/16),CD5 in 13 patients (81.3%, 13/16),CD7 was expressed in 11 patients (68.8%, 11/16),CD8 in 15 patients (93.8%, 15/16),CD4 and CD8 were expressed in 5 patients (31.3%, 5/16),CD4 and CD8 double-negative in patients (6.3%, 1/16),16 patients were CD56 negative (100%, 16/16). (3) TCR gene cloning rearrangement in 16 patients (93.8%, 15/16). (4) EBV-EBER was expressed in 16 patients (100%, 16/16). 11 out of 16 cases died, 1 cese failed to be followed up, 1 case relapsed,and 3 cases survived, reseptively. The media survival time was 4 months. CONCLUSION: Systemic EBV-positive T-cell lymphoma predominantly occurred in childhood and early teen-age, and lacks specific clinic features, usually combined with hemophagocytic syndrome. The confirmed diagnosis requires comprehensive analysis of clinical manifestation, pathomorphology, immunohistochemical detection, EBV-EBER insite hybridization, and TCR gene test. The overall prognosis of the disease is poor and the fatality rate is high.


Assuntos
Infecções por Vírus Epstein-Barr , Linfoma de Células T , Adolescente , Pré-Escolar , Infecções por Vírus Epstein-Barr/complicações , Feminino , Herpesvirus Humano 4 , Humanos , Linfoma de Células T/etiologia , Masculino , Estudos Retrospectivos , Linfócitos T
8.
Acta Haematol ; : 1-7, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31382264

RESUMO

OBJECTIVE: The predictive value of pre-autologous stem cell transplantation (pre-ASCT) positron emission tomography/computed tomography (PET/CT) scans according to different criteria remains elusive in patients with diffuse large B-cell lymphoma (DLBCL). METHODS: A total of 46 DLBCL patients treated with pre-ASCT were enrolled in the present study, and two methods, Deauville score and maximal standardized uptake value reduction (ΔSUVmax), were used to evaluate the PET/CT scans before transplantation. RESULTS: In patients with Deauville 1-3 and ≥4, the 2-year progression-free survival (PFS) rates were 82.8 and 11.8% (p < 0.001), respectively, while the 2-year overall survival (OS) rates were 89.7 and 41.2%, respectively (p < 0.001). When using the ΔSUVmax cut-off of 66% criterion, in patients with a ΔSUVmax of >66 and ≤66%, the 2-year PFS rates were 78.1 and 7.1%, respectively (p < 0.001), while the 2-year OS rates were 87.5 and 35.7%, respectively (p < 0.001). In the univariate analysis, the ΔSUVmax, Deauville score, NCCN-IPI and serum lactate dehydrogenase levels were significantly correlated with the 2-year PFS/OS. Furthermore, the multivariate analysis revealed that the Deauville score was an independent prognostic factor for 2-year PFS. CONCLUSION: The present results indicate that PET/CT scans at pre-ASCT can predict the survival of DLBCL patients, and the Deauville score is better than ΔSUVmax in prognostic prediction.

9.
Adv Mater ; : e1903955, 2019 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-31423664

RESUMO

Lithium-sulfur (Li-S) batteries have arousing interest because of their high theoretical energy density. However, they often suffer from sluggish conversion of lithium polysulfides (LiPS) during the charge/discharge process. Single nickel (Ni) atoms on nitrogen-doped graphene (Ni@NG) with Ni-N4 structure are prepared and introduced to modify the separators of Li-S batteries. The oxidized Ni sites of the Ni-N4 structure act as polysulfide traps, efficiently accommodating polysulfide ion electrons by forming strong Sx 2- ⋅⋅⋅NiN bonding. Additionally, charge transfer between the LiPS and oxidized Ni sites endows the LiPS on Ni@NG with low free energy and decomposition energy barrier in an electrochemical process, accelerating the kinetic conversion of LiPS during the charge/discharge process. Furthermore, the large binding energy of LiPS on Ni@NG also shows its ability to immobilize the LiPS and further suppresses the undesirable shuttle effect. Therefore, a Li-S battery based on a Ni@NG modified separator exhibits excellent rate performance and stable cycling life with only 0.06% capacity decay per cycle. It affords fresh insights for developing single-atom catalysts to accelerate the kinetic conversion of LiPS for highly stable Li-S batteries.

10.
Ying Yong Sheng Tai Xue Bao ; 30(8): 2558-2566, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418178

RESUMO

We explored the stoichiometric characteristic of Artemisia sacrorum communities and its correlation with soil characters by considering the aspect and slope position, and variation of soil nutrient in Yangqingchuan catchment of Wuqi. Our results showed that total carbon, total nitrogen, total phosphorus contents and C:N ratio in A. sacrorum (both shoot and root) in the top of hillock>sunny slope>semi-shady slope>shady slope. The C:P ratio in shoot and the N:P ratio in root decreased consistently. The N:P ratio in shoot and the C:P ratio in root decreased first and then increased. While the total nitrogen and total phosphorus in shoot, and the organic carbon in root increased first and then decreased with the decreases of slope position, the C:N ratio and N:P ratio in shoot decreased first and then increased. The stoichiometric characteristic of A. sacrorum communities was positively correlated with the soil stoichiometric characteristics. However, the C:N ratio, C:P ratio and N:P ratio of A. sacrorum and the total phosphorus of root were negatively correlated to corresponding soil indices. The correlation between shoot nutrition and soil was greater than that between soil and root nutrition. In conclusion, middle position of shady slope was optimal for the growth of A. sacrorum. The stoichiometric characteristics of plants were significantly correlated with soil nutrient condition. Aspect and slope position had a significant effect on the stoichiometric cha-racteristics of A. sacrorum community and soil. Proper A. sacrorum community could help restore soil nutrition.


Assuntos
Artemisia/fisiologia , Solo/química , China , Nitrogênio , Fósforo
11.
Ying Yong Sheng Tai Xue Bao ; 30(8): 2746-2756, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418200

RESUMO

To understand the effects of agricultural management activities on soil arbuscular mycorrhizal (AM) fungi diversity, the high-throughput sequencing based on Illumina MiSeq platform, and the fatty acids fingerprints were used to examine the effects of maize straw returning on soil arbuscular mycorrhizal fungi. The relationships between AM fungal community composition, AM fungal biomass and soil factors after maize straw returning were examined for four continuous years. A total of 2430 operational taxonomic units (OTUs) of AM fungi were classified into 10 genera and 143 species, respectively, which belonged to 1 phylum, 3 classes, 4 orders, 8 families. There was no significant difference in AM fungal community richness (Chaoles index and ACE index) and diversity (Shannon, Simpson diversity indices) in different treatments. Paraglomus and Glomus were dominant genera among all AM fungal communities. With the increase of the maize straw returned amounts, the abundance of Glomus reduced. Under the treatments of 3000 and 9000 kg·hm-2 straw returned, the abundance of Glomus and Acaulospora had significant differences with the control (0 kg·hm-2). Compared with the control, there were significant differences between Archaeospora, Paraglomus and Glomus in the treatment of 3000 kg·hm-2 straw returned. Results from non-metric multi-dimensional scale (NMDS) analysis showed that under 9000 and 12000 kg·hm-2 straw returning treatments, the difference between the ß diversity of soil AM fungi and the spatial distance of controls was farther apart than the other treatments. The effect of straw returning on the ß diversity of AM fungi was significant. The multivariate analysis results revealed the relationship of the spatial variation between the soil physicochemical properties and AM fungi richness and diversity could be explained at 82.8% cumulative variables. The total nitrogen and available nitrogen were the most important factors driving soil microbial communities biomass marked by PLFAs and AM fungal biomass (NLFAs). The continuous maize straw returning to the field changed the genera composition of AM fungi. With the increases of straw returning amounts, the specific species of AM fungi decreased and the similarity between AM fungi community composition decreased. Straw returning increased soil AM fungi biomass and its contribution to soil total microbial biomass.


Assuntos
Micobioma , Micorrizas , Microbiologia do Solo , Solo/química , Zea mays
12.
Neurosci Lett ; 711: 134442, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31442514

RESUMO

Curiosity is an important driving force for human development. This study employed resting-state functional magnetic resonance imaging (fMRI) data (n = 400) and a cross-validation-based predictive framework based on the functional connectivity between and within the default mode network (DMN), fronto-parietal task control network (FPN), and salience network (SN) to explore the relationship between these and curiosity. We found that the curiosity network consisting of functional connections in DMN, FPN, and SN can successfully predict curiosity, and the functional connections within the prefrontal cortex (PFC) and between its nodes and the angular gyrus (AG) made a greater contribution to prediction power. More important, although curiosity has long been considered the main intrinsic motivation of learning and self-efficacy (individual's confidence in his or her own ability) is the primary factor affecting learning motivation, few studies have explored the relationship between the two. Therefore, we speculated that the relationship between the curiosity brain network and curiosity might also be related to self-efficacy. In this study, the strength of the curiosity network was calculated as a mediation variable to explore the relationship between self-efficacy and curiosity. The results revealed that the strength of the curiosity brain network mediates the association between self-efficacy and curiosity score. Our findings suggest that self-efficacy plays an important role in shaping individuals' trait curiosity and extends understanding of the neural mechanism of curiosity.

13.
Ying Yong Sheng Tai Xue Bao ; 30(6): 2056-2062, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31257779

RESUMO

The endophytic bacteria were isolated from the roots of polycyclic aromatic hydrocarbon (PAHs)-tolerant plant. We investigated their ability of PAHs degradation and plant growth promo-ting, with the aim to provide theoretical support for bacterial-plant cooperative soil remediation. Kochia scoparia living in coking plant area were selected for strains isolation. Eight endophytic bacteria strains, which used pyrene and 1-aminocyclopropane-1-carboxylic acid (ACC) as sole carbon and nitrogen source, were isolated from the roots of K. scoparia. Three endophytic bacteria, KSE4, KSE7 and KSE8, displayed high degradation efficiency in pyrene degradation experiment. They were identified as Bacillus sp., Pseudomonas sp., and Sphingobacterium sp., respectively. The abili-ties of those three strains to produce ACC deaminase and their effects on seed germination of K. scoparia were examined under pyrene stress through liquid culture tests. The results showed that the activity of ACC deaminase decreased with increasing pyrene concentration (0-15 mg·L-1). KSE7 had the strongest promotion effect. When pyrene concentration reached to 15 mg·L-1, the germination rate and bud length of K. scoparia increased by 44.8% and 61.1%, respectively. Our results indicated that KSE7 is a promising bacterial strain for soil remediation in coking plant area.


Assuntos
Bassia scoparia/metabolismo , Biodegradação Ambiental , Raízes de Plantas/microbiologia , Pirenos/metabolismo , Poluentes do Solo/metabolismo , Bactérias , Hidrocarbonetos Policíclicos Aromáticos
14.
J Neurol Sci ; 404: 86-90, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31352294

RESUMO

BACKGROUND AND PURPOSE: To investigate the influence of carotid artery stenting (CAS) with embolic protection device (EPD) on the cognitive function of patients with near-occlusion of the cervical internal carotid artery (ICA). METHODS: From February 2014 to December 2017, a total of 79 symptomatic patients were recruited in this study. Of these patients, 31 patients refused to receive CAS therapy. They were divided into the CAS group (48 patients) and the medical treatment group (31 patients). Montreal cognitive assessment (MoCA) instrument was used for the evaluation of cognitive function. The analyzed endpoints included cumulative 12 month incidence of ipsilateral ischemic cerebrovascular events and MoCA scores at 1 month and 12 months after treatment. RESULTS: Cumulative 12 months incidence of ipsilateral ischemic cerebrovascular events was lower in patients who underwent CAS than in patients on medical treatment (P < 0.05). In CAS group, the total MoCA score, scores of attention and delayed recall at months 1 and 12 increased when compared with those at baseline (P < 0.05). In medical treatment group, the total MoCA score and attention score at month 12 decreased when compared with those at baseline (P < 0.05). In CAS group, the total MoCA score, scores of line connection test, drawing clock, attention and delayed recall were improved at 1 and 12 months when compared with medical treatment at the same time points (P < 0.05). CONCLUSIONS: CAS with EPD not only decreases the risk of ipsilateral TIA and stroke but also may improve the cognitive function of symptomatic patients with ICA near-occlusion.

15.
Biomed Pharmacother ; 118: 109120, 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31302419

RESUMO

PROBLEM AND OBJECT: 5-fluorouracil (5-FU) is a pyrimidine-like antimetabolite. It has been widely used in human cancer therapies; however, the drug sensitivity can be very low and the survival outcome can be very poor dependent on tumor types and individual heterogeneity. This research was aimed to study the effects of matrix metalloproteinase 1 (MMP1) gene on nasopharyngeal carcinoma (NPC) cell proliferation, viability, invasiveness, apoptosis, and sensitivity to 5-FU. METHODS: Bioinformatics method was used to screen out the differentiated expressed genes in nasopharyngeal carcinomas compared with normal nasopharyngeal epithelial tissues. qRT-PCR was used to determine the expression of MMP1 mRNA, and western blot was used to determine the protein expression of MMP1, thymidylate synthase (TS), and dihydropyrimidine dehydrogenase (DPD). Colony foci formation assay, CCK8 assay, and BrdU incorporation assay were used to study the cell proliferation. Transwell invasion assay was carried out to determine cell invasion. Flow cytometry and caspase 3/7 activation assay were used to detect cell apoptosis. RESULTS: MMP1 gene was the most significantly upregulated gene in nasopharyngeal carcinomas according to the bioinformatics analysis. And the upregulation of MMP1 gene was confirmed in both NPC tissues and cell lines using RT-QPCR and western blot technique. When 5-FU was not a player, the forced overexpression of MMP1 gene led to enhanced growth and invasion of CNE1 and HNE1 cell lines, whereas MMP1 gene knockdown resulted in the opposite outcome. When 5-FU was added, MMP1 gene knockdown led to significantly suppressed cell proliferation and enhanced cell apoptosis. Also, MMP1 gene knockdown caused significantly lower level of TS and DPD enzymes. CONCLUSION: Not only the knockdown of MMP1 gene led to suppressed proliferation and invasion but also increased the sensitivity to 5-FU of CNE1 and HNE1 cells. Our results provided convincing evidence that MMP1 gene knockdown could offer a favorable sensitivity approach for NPC with 5-FU treatment.

16.
Medicine (Baltimore) ; 98(30): e16572, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31348287

RESUMO

INTRODUCTION: Epithelioid haemangioendothelioma (EHE) is a rare vascular neoplasm that primarily occurs in the soft tissues of the extremities, with "intermediate" or "borderline" malignant biological characteristics and a propensity for recurrence and metastasis. Oral cavity involvement is extremely rare, with only 5 reported cases of intraosseous EHE of the mandible. Herein, we report an additional case and review the related literature. PATIENT CONCERNS: A 44-year-old man was referred to our institution with a 2-month history of left lower lip numbness and a 2-week history of painful swelling of the left mandible. He had received antibiotic treatment, which failed to ameliorate his symptoms. His medical and family histories were not significant. DIAGNOSIS: Enhanced computed tomography revealed a poorly defined, 4.5 × 3.5 × 1.0 cm osteolytic lesion in the angle and ramus of the mandible, as well as an eroded lingual cortex. Histopathological examination confirmed a diagnosis of EHE. INTERVENTIONS: Surgical resection was performed via segmental mandibulectomy and the defect was reconstructed using a vascularized fibular flap. OUTCOMES: The patient did not exhibit signs of locoregional recurrence after 3 years of follow-up. CONCLUSIONS: Intraosseous EHE of the mandible is an unpredictable lesion with a relatively benign course; however, the few reported cases of this disease do not highlight any factors that can predict the risk of locoregional recurrence or metastasis. There is also no consensus regarding the optimal treatment for intraosseous EHE; however, we recommend extensive local excision with close clinical follow-up.


Assuntos
Hemangioendotelioma Epitelioide/patologia , Mandíbula/patologia , Adulto , Hemangioendotelioma Epitelioide/cirurgia , Humanos , Masculino , Mandíbula/cirurgia , Osteotomia Mandibular
17.
Allergy ; 2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-31267532

RESUMO

BACKGROUND: Germline-encoded innate immune pattern recognition receptors (PRR) are expressed at epithelial surfaces and modulate epithelial defenses. Evidence suggests that stimulation of the Toll-like receptor (TLR) family of PRR may regulate epithelial barrier integrity by upregulating tight junction (TJ) complex protein expression, but it is not known whether this mechanism is utilized in esophageal epithelial cells. TJ complex proteins maintain intact barrier function and are dysregulated in atopic disorders including eosinophilic esophagitis. METHODS: Pattern recognition receptors expression was assessed in EoE and control primary esophageal epithelial cells, demonstrating robust expression of TLR2 and TLR3. The three-dimensional air-liquid interface culture (ALI) model was used to test whether TLR2 or TLR3 stimulation alters epithelial barrier function using an in vitro model of human epithelium. Transepithelial electrical resistance (TEER) and FITC-Dextran permeability were evaluated to assess membrane permeability. ALI cultures were evaluated by histology, immunohistochemistry, Western blotting, and chromatin immunoprecipitation (ChIP). RESULTS: TLR3 stimulation did not change TEER in the ALI model. TLR2 stimulation increased TEER (1.28- to 1.31-fold) and decreased paracellular permeability to FITC-Dextran, and this effect was abolished by treatment with anti-TLR2 blocking antibody. TJ complex proteins claudin-1 and zonula occludens-1 were upregulated following TLR2 stimulation, and ChIP assay demonstrated altered histone 4 acetyl binding at the TJP1 enhancer and CLDN1 enhancer and promoter following zymosan treatment, implying the occurrence of durable chromatin changes. CONCLUSIONS: Our findings implicate the TLR2 pathway as a potential regulator of esophageal epithelial barrier function and suggest that downstream chromatin modifications are associated with this effect.

18.
Chem Commun (Camb) ; 55(62): 9184-9187, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31305803

RESUMO

Herein, ultrasmall MnO nanoparticles encapsulated by nitrogen-rich doped carbon matrix hybrids are synthesized by annealing ethylenediaminetetraacetic manganese [Mn2(EDTA)] precursors for the first time. The resulting MnO@NDC composite exhibits excellent rate capability and outstanding cycling stability as an advanced anode material for lithium-ion batteries.

19.
Food Chem ; 299: 125095, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31279124

RESUMO

Hydrogen gas (H2), a multifunctional signaling molecule, has received increasing attention in recent years. In the present study, hydrogen-rich water (HRW) (2 ppm) was used for the processing of sprouted black barley (Hordeum distichum L.), and the results showed that the HRW treatment could significantly increase the germination rate and growth rate of black barley (P < 0.05). A chemical component analysis showed that in sprouted black barley, the HRW treatment could change the distribution of phytochemicals (e.g., the ionic strength of guanosine), increase the concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, Ca and Fe and the hydroxyl radical scavenging rate, and decrease the protein, fat, starch and dietary fibre contents compared with the results obtained after treatment with ultra-pure water (P < 0.05). HRW can be used for the processing of sprouted grains to effectively increase their germination efficiency and concentrations of bioactive phytochemicals.

20.
J Periodontol ; 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31347700

RESUMO

BACKGROUND: Serum amyloid A (SAA) has been identified to trigger inflammation response, and play a crucial role in chronic inflammatory diseases. However, the regulatory mechanism of SAA still remains unclear during the development of periodontitis METHODS: SAA mRNA and protein expression were detected in healthy and inflammatory gingival tissues using real-time polymerase chain reaction (PCR) and immunohistochemistry. Human recombinant SAA (Apo-SAA), Pam3CSK4 (a Toll-like receptor (TLR) 2 ligand), siRNA-SAA, or TLR2 neutralizing antibody was applied to treat human gingival fibroblasts, respectively, or combined. SAA, TLRs, and inflammatory cytokines interleukin (IL)-6 and IL-8 were analyzed by real-time PCR, western blotting, or enzyme-linked immunosorbent assay. RESULTS: SAA expression increased in human inflammatory gingival tissues from patients with periodontitis (P <0.05). Apo-SAA could increase not only the mRNA expression of TLR2 (P <0.05), but also IL-6 and IL-8 mRNA and protein levels (P <0.05) which was suppressed by TLR2 antibody in human gingival fibroblasts. Pam3CSK4 increased SAA, IL-6, and IL-8 levels (P <0.05). However, the expression of SAA, IL-6, and IL-8 decreased after transfection of siRNA-SAA (P <0.05). CONCLUSION: SAA not only increases in inflammatory gingiva, but also triggers inflammatory cytokine secretion via interacting with TLR2 pathway in human gingival fibroblasts, which indicates that SAA is involved in periodontal inflammation.

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