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2.
Biomed Res Int ; 2020: 3650935, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33354565

RESUMO

Ischemic stroke (IS) greatly threatens human health resulting in high mortality and substantial loss of function. Recent studies have shown that the outcome of IS has sex specific, but its mechanism is still unclear. This study is aimed at identifying the sexually dimorphic to peripheral immune response in IS progression, predicting potential prognostic biomarkers that can lead to sex-specific outcome, and revealing potential treatment targets. Gene expression dataset GSE37587, including 68 peripheral whole blood samples which were collected within 24 hours from known onset of symptom and again at 24-48 hours after onset (20 women and 14 men), was downloaded from the Gene Expression Omnibus (GEO) datasets. First, using Bioconductor R package, two kinds of differentially expressed genes (DEGs) (nonsex-specific- and sex-specific-DEGs) were screened by follow-up (24-48 hours) vs. baseline (24 hours). 30 nonsex-specific DEGs (1 upregulated and 29 downregulated), 79 female-specific DEGs (25 upregulated and 54 downregulated), and none of male-specific DEGs were obtained finally. Second, bioinformatics analysis of female-specific DEGs was performed. Gene Ontology (GO) functional annotation analysis shows that DEGs were mainly enriched in translational initiation, cytosolic ribosome, and structural constituent of ribosome. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis shows that the top 6 enrichment pathways are ribosome, nuclear factor--kappa B (NF-kappa B) signaling pathway, apoptosis, mineral absorption, nonalcoholic fatty liver disease, and pertussis. Three functional modules were clustered in the protein-protein interaction (PPI) network of DEGs. The top 10 key genes of the PPI network constructed were selected, including RPS14, RPS15A, RPS24, FAU, RPL27, RPL31, RPL34, RPL35A, RSL24D1, and EEF1B2. Sex difference of ribosome in stroke-induced peripheral immunosuppression may be the potential mechanism of sex disparities in outcome after IS, and women are more likely to have stroke-induced immunosuppression. RPS14, RPS15A, RPS24, FAU, RPL27, RPL31, RPL34, RPL35A, RSL24D1, and EEF1B2 may be novel prognostic biomarkers and potential therapeutic targets for IS.

3.
Clin Lab ; 66(6)2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32538042

RESUMO

BACKGROUND: As the mechanism of systemic inflammatory response in the course of cancer progression is gradually revealed, research has begun to focus on the two indictors of neutrophil to lymphocyte ratio (NLR) and the platelet to lymphocyte ratio (PLR) which may be associated with clinical disease development, treatment, and prognosis in patients who are undergoing surgery, chemotherapy, targeted therapy, and immunotherapy. We aim to define the clinical application values of those two biomarkers in multiple cancers. METHODS: PubMed and Web of Science are used to perform the systematic literature research. Related articles and references were identified for analyzing the association of between NLR and PLR with treatment outcome, as well as progression of cancers. RESULTS: NLR and PLR are convenient, easy to calculate, economical, and practical biomarkers, effectively predicting treatment outcome and risk of death based on inflammatory cells. Elevated NLR and PLR are significantly in line with worse clinical pathological characteristics, deeper invasiveness, more lymph node metastasis and advanced TNM stage. A significant association was observed that high NLR and PLR predict poor overall survival and disease-free survival. CONCLUSIONS: NLR and PLR can be used as available biomarkers in prognostic survival and formulation of treatment strategy of multiple cancers.

4.
Aging (Albany NY) ; 12(4): 3558-3573, 2020 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-32087603

RESUMO

RNA binding proteins (RBPs) dysregulation have been reported in various malignant tumors and associated with the occurrence and development of cancer. However, the role of RBPs in lung adenocarcinoma (LUAD) is poorly understood. We downloaded the RNA sequencing data of LUAD from the Cancer Genome Atlas (TCGA) database and determined the differently expressed RBPs between normal and cancer tissues. The study then systemically investigated the expression and prognostic value of these RBPs by a series of bioinformatics analysis. A total of 223 differently expressed RBPs were identified, including 101 up-regulated and 122 down-regulated RBPs. Eight RBPs (IGF2BP1, IFIT1B, PABPC1, TLR8, GAPDH, PIWIL4, RNPC3, and ZC3H12C) were identified as prognosis related hub gene and used to construct a prognostic model. Further analysis indicated that the patients in the high-risk subgroup had poor overall survival(OS) compared to those in low-risk subgroup based on the model. The area under the curve of the time-dependent receiver operator characteristic curve of the prognostic model are 0.775 in TCGA cohort and 0.814 in GSE31210 cohort, confirming a good prognostic model. We also established a nomogram based on eight RBPs mRNA and internal validation in the TCGA cohort, which displayed a favorable discriminating ability for lung adenocarcinoma.

5.
Huan Jing Ke Xue ; 39(10): 4825-4833, 2018 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-30229633

RESUMO

Diethyl phthalate (DEP) is a plastic additive that entered the soil environment due to the extensive use of plastic products. However, its toxicity to soil animals and the associated toxicity mechanism were not completely understood. Eisenia foetida was selected as the research object and exposed to simulated contaminated soil with different concentrations of DEP. Antioxidant enzyme activity, reactive oxygen species (ROS) content, Glutathione-S-Transferase (GST) activity, Malondialdehyde (MDA) content and amount of DNA damage in the earthworms were used as evaluation parameters for the study. The results showed that under DEP stress, the activities of antioxidant enzymes, GST and ROS in earthworms changed and resulted in gene damage. Under the stress of 0.1-50 mg·kg-1 DEP exposure during the 28 d experiment, the level of ROS increased and there was a "dose-effect" relationship. Excessive ROS gave rise to an increase of MDA content in the body from lipid peroxidation. Under the combined action of ROS and MDA, DNA in the body cavity of earthworm was damaged and there was also a "dose-effect" relationship between the degree of damage and the concentration of DEP. In summary, DEP may cause a certain degree of damage to organisms, with damage to the DNA of earthworms representing fairly strong eco-toxicological effects. Therefore, adequate attention should be paid to DEP disposal.


Assuntos
Dano ao DNA , Oligoquetos/efeitos dos fármacos , Estresse Oxidativo , Ácidos Ftálicos/toxicidade , Poluentes do Solo/toxicidade , Animais , Catalase/metabolismo , Glutationa Transferase/metabolismo , Malondialdeído/metabolismo , Oligoquetos/enzimologia , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
6.
Chin J Integr Med ; 23(4): 245-252, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27484764

RESUMO

Chinese medicine (CM) has been used in clinical treatment for thousands of years in China, Japan, Korea, and other countries. CM is at present attracting many attentions around the world for reproductive health care and disease prevention, including treatment of female infertility. This review focuses on the CM treatment for female infertility patients, and supplies a summary on the efficacy, safety, and mechanism of some Chinese herbal medicines, herbal medicine-derived active compounds, and acupuncture. A large number of researches have reported that CM could alleviate or even cure female infertility by regulating hormone, improving reproductive outcome of in vivo fertilization, affecting embryonic implantation, curing polycystic ovarian syndrome, endometriosis, pelvic inflammatory disease, relieving mental stress, and regulating immune system. Meanwhile, a few studies claimed that there was little adverse reaction of CM in randomized controlled trials. However, up to present there is a lack of adequate evidences with molecular mechanistic researches and randomized controlled trials to prove the CM as an effective and safe treatment for infertility. Thus, utility of CM as a complementary medicine will be a feasible method to improve the outcome of female infertility treatment.


Assuntos
Terapias Complementares , Infertilidade Feminina/terapia , Medicina Tradicional Chinesa , Implantação do Embrião , Feminino , Fertilização In Vitro , Hormônios/metabolismo , Humanos , Infertilidade Feminina/imunologia
7.
Environ Sci Pollut Res Int ; 23(18): 18552-63, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27294701

RESUMO

This study investigated the treatment performance for aging leachate containing refractory organic pollutants by TiO2-organobentonite photocatalyst combined with polyaluminum chloride (PAC) coagulant. TiO2 was immobilized on organobentonite granules as a supporter modified by cetyltrimethylammonium chloride (CTAC). The prepared catalysts were characterized by ESEM, FTIR, and XRD analysis, which showed that TiO2-organobentonite catalyst had uniform coating of TiO2 on support. Chemical oxygen demand (COD) and NH3-N removal rates by combination of TiO2-CTAC2.0 photocatalysis and PAC coagulation were evaluated, optimized, and compared to that by either treatment alone, with respect to TiO2-CTAC2.0 dose, photocatalytic contact time, pH, and PAC dose. Furthermore, higher removal rates (COD 80 %; NH3-N 46 %) were achieved by response surface methodology (RSM) when TiO2-CTAC2.0 photocatalysis was followed by PAC coagulation at optimized conditions. The optimized experimental conditions were TiO2-CTAC2.0 dosage of 5.09 g/L, at pH 5.53, photocatalytic contact time for 180 min, and PAC dosage of 1062 mg/L.


Assuntos
Compostos de Bis-Trimetilamônio/química , Eliminação de Resíduos/métodos , Titânio/química , Poluentes Químicos da Água/química , Hidróxido de Alumínio/análise , Bentonita/química , Análise da Demanda Biológica de Oxigênio , Compostos de Bis-Trimetilamônio/análise , Catálise , Cetrimônio , Compostos de Cetrimônio , Coagulantes , Poluentes Químicos da Água/análise
8.
Medicine (Baltimore) ; 95(24): e3941, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27311006

RESUMO

The main obstacle to achieving an R0 resection after a major hepatectomy is inability to preserve an adequate future liver remnant (FLR) to avoid postoperative liver failure (PLF). Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) is a novel technique for resecting tumors that were previously considered unresectable, and this technique results in a vast increase in the volume of the FLR in a short period of time. However, this technique continues to provoke heated debate because of its high mortality and morbidity.The evolution of ALPPS and its advantages and disadvantages have been systematically reviewed and evaluated in accordance with current evidence. Electronic databases (PubMed and Medline) were searched for potentially relevant articles from January 2007 to January 2016.ALPPS has evolved into various modified forms. Some of these modified techniques have reduced the difficulty of the procedure and enhanced its safety. Current evidence indicates that the advantages of ALPPS are rapid hypertrophy of the FLR, the feasibility of the procedure, and a higher rate of R0 resection in comparison to other techniques. However, ALPPS is associated with worse major complications, more deaths, and early tumor recurrence.Hepatobiliary surgeons should carefully consider whether to perform ALPPS. Some modified forms of ALPPS have reduced the mortality and morbidity of the procedure, but they cannot be recommended over the original procedure currently. Portal vein embolization (PVE) is still the procedure of choice for patients with a tumor-free FLR, and ALPPS could be used as a salvage procedure when PVE fails. More persuasive evidence needs to be assembled to determine whether ALPPS or two-stage hepatectomy (TSH) is better for patients with a tumor involving the FLR. Evidence with regard to long-term oncological outcomes is still limited. More meticulous comparative studies and studies of the 5-year survival rate of ALPPS could ultimately help to determine the usefulness of ALPPS. Indications and patient selection for the procedure need to be determined.


Assuntos
Hepatectomia/métodos , Neoplasias Hepáticas/cirurgia , Veia Porta/cirurgia , Procedimentos Cirúrgicos Vasculares/métodos , Humanos , Ligadura , Neoplasias Hepáticas/irrigação sanguínea
9.
World J Gastroenterol ; 22(1): 262-74, 2016 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-26755875

RESUMO

The prevalence of hepatocellular carcinoma (HCC) worldwide parallels that of persistent infection with the hepatitis B virus (HBV) and/or hepatitis C virus (HCV). According to recommendations by the World Health Organization guidelines for HBV/HCV, alpha-fetoprotein (AFP) testing and abdominal ultrasound should be performed in routine surveillance of HCC every 6 mo for high-risk patients. These examinations have also been recommended worldwide by many other HCC guidelines over the past few decades. In recent years, however, the role of AFP in HCC surveillance and diagnosis has diminished due to advances in imaging modalities. AFP was excluded from the surveillance and/or diagnostic criteria in the HCC guidelines published by the American Association for the Study of Liver Diseases in 2010, the European Association for the Study of the Liver in 2012, and the National Comprehensive Cancer Network in 2014. Other biomarkers, including the Lens culinaris agglutinin-reactive fraction of AFP (AFP-L3), des-γ-carboxyprothrombin, Dickkopf-1, midkine, and microRNA, are being studied in this regard. Furthermore, increasing attention has focused on the clinical utility of biomarkers as pre-treatment predictors for tumor recurrence and as post-treatment monitors. Serum and tissue-based biomarkers and genomics may aid in the diagnosis of HCC, determination of patient prognosis, and selection of appropriate treatment. However, further studies are needed to better characterize the accuracy and potential role of these approaches in clinical practice.


Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma Hepatocelular/diagnóstico , Neoplasias Hepáticas/diagnóstico , Biomarcadores , Carcinoma Hepatocelular/etiologia , Carcinoma Hepatocelular/metabolismo , Hepatite B/complicações , Hepatite C/complicações , Humanos , Neoplasias Hepáticas/etiologia , Neoplasias Hepáticas/metabolismo , Prognóstico , Precursores de Proteínas , Protrombina , alfa-Fetoproteínas/metabolismo
10.
J Hazard Mater ; 286: 493-502, 2015 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-25603298

RESUMO

Improving the reduction kinetics is crucial in the electroreduction process of Cr(VI). In this study, we developed a novel adsorption-electroreduction system for accelerated removal of Cr(VI) by employing reticulated vitreous carbon electrode modified with sulfuric acid-glycine co-doped polyaniline (RVC/PANI-SA-GLY). Firstly, response surface methodology confirmed the optimum polymerization condition of co-doped polyaniline for modifying electrodes (Aniline, sulfuric acid and glycine, respectively, of 0.2 mol/L, 0.85 mol/L, 0.93 mol/L) when untraditional dopant glycine was added. Subsequently, RVC/PANI-SA-GLY showed higher Cr(VI) removal percentages in electroreduction experiments over RVC electrode modified with sulfuric acid doped polyaniline (RVC/PANI-SA) and bare RVC electrode. In contrast to RVC/PANI-SA, the improvement by RVC/PANI-SA-GLY was more significant and especially obvious at more negative potential, lower initial Cr(VI) concentration, relatively less acidic solution and higher current densities, best achieving 7.84% higher removal efficiency with entire Cr(VI) eliminated after 900 s. Current efficiencies were likewise enhanced by RVC/PANI-SA-GLY under quite negative potentials. Fourier transform infrared (FTIR) and energy dispersive spectrometer (EDS) analysis revealed a possible adsorption-reduction mechanism of RVC/PANI-SA-GLY, which greatly contributed to the faster reduction kinetics and was probably relative to the absorption between protonated amine groups of glycine and HCrO4(-). Eventually, the stability of RVC/PANI-SA-GLY was proven relatively satisfactory.


Assuntos
Compostos de Anilina/química , Carbono/química , Cromo/química , Glicina/química , Ácidos Sulfúricos/química , Adsorção , Eletrodos , Oxirredução , Eliminação de Resíduos Líquidos/métodos
11.
Zhonghua Zhong Liu Za Zhi ; 34(3): 196-200, 2012 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-22780973

RESUMO

OBJECTIVE: To evaluate the expression of BRCA1, ERCC1, TUBB3 and PRR13 mRNA and their relationship with clinical chemosensitivity in primary ovarian cancer, and to assess the predictive value of joint detection of both BRCA1 and ERCC1 genes for the treatment of primary ovarian cancer. METHODS: Primary epithelial ovarian tumor samples were collected from 46 patients who underwent cytoreductive surgery. Real-time quantitative PCR was used to analyze the relative expression of BRCA1, ERCC1, TUBB3 and PRR13 mRNA in those cases. The correlation of clinical chemosensitivity and the test results was statistically analyzed. The efficacy of the joint prediction of clinical chemosensitivity by combining the two drug resistance gene detection was evaluated. RESULTS: The BRCA1 mRNA relative expression logarithm in the clinical-resistant group was 0.673±2.143, and clinical-sensitive group -1.436±2.594 (P=0.008). The ERCC1 mRNA relative expression logarithm in the clinical-resistant group was -0.529±1.982 and clinical-sensitive group -3.188±2.601 (P=0.001). BRCA1 and ERCC1 expression level is negatively correlated with platinum-based chemosensitivity. The PRR13 expressions in the two groups were not significantly different (P=0.074), and the TUBB3 expressions between the two groups were also not significantly different (P=0.619). When the intercept point value BRCA1 mRNA expression logarithm was -0.6, the predictive sensitivity, specificity, positive predictive value and negative predictive value were 73.3%, 75.0%, 84.6% and 60.0%, respectively, with the best comprehensive assessment. When the intercept point value of ERCC1 mRNA expression logarithm was -1, the predictive sensitivity, specificity, positive predictive value and negative predictive value were 80.0%, 68.8%, 82.8% and 64.7%, respectively, with the best comprehensive assessment. The combination detection of BRCA1 and ERCC1 can improve the chemotherapeutic sensitivity, specificity, positive predictive value and negative predictive value to 86.7%, 68.8%, 83.9% and 73.3%, respectively. CONCLUSIONS: BRCA1 and ERCC1 mRNA expression has a negative correlation with the clinical sensitivity of platinum-based chemotherapy. Combination detection of the two drug-resistance associated genes can improve the predictive efficacy of ovarian cancer chemosensitivity and beneficial to individual treatment of ovarian cancer.


Assuntos
Proteína BRCA1/metabolismo , Proteínas de Ligação a DNA/metabolismo , Resistencia a Medicamentos Antineoplásicos , Endonucleases/metabolismo , Neoplasias Epiteliais e Glandulares/metabolismo , Neoplasias Ovarianas/metabolismo , Proteínas Repressoras/metabolismo , Tubulina (Proteína)/metabolismo , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Proteína BRCA1/genética , Antígeno Ca-125/sangue , Carboplatina/administração & dosagem , Carcinoma Epitelial do Ovário , Proteínas de Ligação a DNA/genética , Endonucleases/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Epiteliais e Glandulares/tratamento farmacológico , Neoplasias Epiteliais e Glandulares/cirurgia , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/cirurgia , Paclitaxel/administração & dosagem , RNA Mensageiro/metabolismo , Proteínas Repressoras/genética , Tubulina (Proteína)/genética
12.
Zhonghua Fu Chan Ke Za Zhi ; 46(3): 193-8, 2011 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-21575453

RESUMO

OBJECTIVE: To predict clinical chemotherapy sensitivity of primary ovarian cancer by jointing adenosine triphosphate (ATP)-tumor chemo-sensitivity assay (TCA) method in vitro and detection of drug resistance genes, provide reference for clinical treatment. METHODS: Forty-seven primary epithelial ovarian tumor samples were collected from the patients who received cytoreductive surgery. Viable ovarian cancer cells obtained from malignant tissue were tested for their sensitivity to carboplatin (CBP), cisplatin (DDP), paclitaxel (PTX) and CBP + PTX using ATP-TCA method in vitro; at same time, real-time quantitative PCR was used to analysis BRCA1 and ERCC1 mRNA relative expression in forty-six specimens (1 frozen tumor samples mRNA were not detected due to serious degradation). The relationship between ATP-TCA test results, clinical indicators, and the effectiveness of the joint prediction on clinical chemo-sensitivity by combining these two methods were statistically analyzed using chi-square test. RESULTS: (1) The results showns that three programs of DDP, CBP and PTX + CBP were significantly related with clinical results (P < 0.05) in vitro, in which the compliance rate in PTX + CBP program was the highest 83% (39/47), and the predictive sensitivity, predictive specificity, positive predictive value, negative predictive value and predictive accurate rate were 90%, 71%, 84% and 80%, respectively. PTX + CBP combined in vitro test results was also related with residual tumor size and neoadjuvant chemotherapy, which was more prone to drug resistance with residual tumor larger than 2 cm (P = 0.023) and with neoadjuvant chemotherapy (P = 0.011). (2) BRCA1 mRNA expression levels in the clinical-resistant group and the clinical-sensitive group was 0.673 ± 2.143 and -1.436 ± 2.594 (P = 0.008), ERCC1 mRNA expression levels in the clinical-resistant group and the clinical-sensitive group was -0.529 ± 1.982 and -3.188 ± 2.601 (P = 0.001). There were also significant correlation among the expression levels of BRCA1, ERCC1 mRNA and clinical efficacy (P < 0.01). (3) ATP-TCA and detection of drug resistance genes combined to predict the clinical application of PTX + CBP resistance may occur in 8/9 cases. CONCLUSIONS: ATP-TCA may be an ideal method of in vitro drug sensitivity testing method, which could effectively predict clinical chemotherapy sensitivity. Combination of the drug-resistant associated genes detection method and the ATP-TCA method can increase the predictive effectiveness of ovarian cancer chemosensitivity and guide individual chemotherapy of ovarian cancer.


Assuntos
Trifosfato de Adenosina/metabolismo , Antineoplásicos/farmacologia , Proteína BRCA1/metabolismo , Proteínas de Ligação a DNA/metabolismo , Endonucleases/metabolismo , Neoplasias Ovarianas/tratamento farmacológico , Adulto , Idoso , Antineoplásicos/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Proteína BRCA1/genética , Antígeno Ca-125/sangue , Cisplatino/farmacologia , Proteínas de Ligação a DNA/genética , Resistencia a Medicamentos Antineoplásicos , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Endonucleases/genética , Feminino , Humanos , Pessoa de Meia-Idade , Neoplasias Ovarianas/metabolismo , Neoplasias Ovarianas/patologia , Paclitaxel/farmacologia , Valor Preditivo dos Testes , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Análise de Regressão , Sensibilidade e Especificidade
13.
Am J Obstet Gynecol ; 204(6): 544.e9-17, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21497788

RESUMO

OBJECTIVE: The objective of the study was to investigate the expression and regulation of polycomb group (PcG) proteins in human neural tube defects (NTDs). STUDY DESIGN: PcG proteins in human NTD fetuses and age-matched controls were detected by Western blot. The relation between PcG proteins and microribonucleic acids was predicted and confirmed by the bioinformatics method, real-time polymerase chain reaction (PCR), dual-luciferase activity assay, and Western blot. The trimethyl condition of histone H3 Lys27 (H3K27) was detected by immunohistochemical and immunofluorescence. RESULTS: Embryonic ectoderm development protein (EED) was differentially detected in placenta, cerebral cortex, and spinal cord from NTDs and age-matched controls. MiR-30b can interact with 3'-untranslated region (UTR) of Eed and regulate endogenous EED expression in neural tissues. In addition, we found an inverse relationship between the miR-30b expression and the amount of trimethyl H3K27. CONCLUSION: Differential expression of EED exists in the nerves system in human NTDs and that is regulated by miR-30b.


Assuntos
MicroRNAs/fisiologia , Defeitos do Tubo Neural/genética , Proteínas Repressoras/genética , Adulto , Feminino , Feto , Regulação da Expressão Gênica , Humanos , Masculino , Complexo Repressor Polycomb 2
14.
Int J Mol Sci ; 11(2): 719-30, 2010 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-20386663

RESUMO

The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. There were reports to show that some microRNAs (miRNAs) may play a key role during embryo implantation in mouse. However, the miR-320 expression profiles in the rat uterus during peri-implantation are unknown. In the present study, we found that the expression level of miR-320 was lower on day 5 of gestation (g.d. 5) in rats than g.d.3 and g.d.4 and restored gradually from g.d.6. MiR-320 was specifically localized in glandular and luminal epithelia and decidua. The expression of miR-320 was not significantly different in the pseudopregnant uterus and decreased in the uteri of rats subjected to activation of delayed implantation. Artificial decidualization and treatment with progesterone increased the miR-320 expression. Thus, miR-320 was differentially expressed in the rat uterus during implantation. The expression level was affected by active blastocysts and decidualization during the window of implantation. Steroid hormones, progesterone stimulated miR-320 expression.


Assuntos
Implantação do Embrião , Regulação da Expressão Gênica/efeitos dos fármacos , MicroRNAs/genética , MicroRNAs/metabolismo , Útero/metabolismo , Animais , Blastocisto/metabolismo , Decídua/metabolismo , Implantação do Embrião/genética , Feminino , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Idade Gestacional , Gravidez , Progesterona/farmacologia , Progestinas/farmacologia , Ratos , Ratos Sprague-Dawley , Útero/efeitos dos fármacos
15.
Zhonghua Zhong Liu Za Zhi ; 32(11): 855-8, 2010 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-21223693

RESUMO

OBJECTIVE: To explore the value of adenosine triphosphate-tumor chemosensitivity assay (ATP-TCA) in individualized treatment of recurrent epithelial ovarian cancer (REOC), and to evaluate the correlation between the in vitro chemosensitivity assay and clinical drug sensitivity. METHODS: Sixty-nine REOC specimens were tested by ATP-TCA assay retrospectively. The patients were divided into strong sensitive, moderate sensitively and resistant groups according to the ATP-TCA assay results. The clinical results were evaluated according to imaging and serum CA125 analysis. The correlation between in vitro ATP-TCA assay and clinical outcome was statistically analyzed by χ(2) test. The progression free survival (PFS) and overall survival (OS) of each group were analyzed using Kaplan-Meier method. RESULTS: The results of ATP-TCA assay had significant correlation with clinical outcome. The clinical chemotherapy outcome became better with increased drug sensitivity in vitro (χ(2) = 9.066, P = 0.004). The sensitivity, specificity, positive predictive value, negative predictive value and accuracy rate for ATP-TCA method to predict the clinical chemotherapy sensitivity of REOC were 87.5%, 45.9%, 58.3%, 80.9% and 65.2%, respectively. The mean PFS of strong sensitive group, moderately sensitive group and resistant group were 187.1 days, 195.0 days and 60.3 days, respectively. The mean OS were 476.7, 335.7 and 237.5 days, respectively, following the start of TCA-directed therapy. The PFS and OS of the two sensitivity groups in vitro were significantly longer than that of the in vitro-resistant group (P < 0.01). CONCLUSION: The results of ATP-TCA assay are well correlated with clinical treatment responses. The assay may be an important and useful method for individualized chemotherapy for recurrent ovarian cancer.


Assuntos
Trifosfato de Adenosina/metabolismo , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Cistadenocarcinoma Seroso/tratamento farmacológico , Neoplasias Ovarianas/tratamento farmacológico , Antígeno Ca-125/sangue , Carcinoma Endometrioide/sangue , Carcinoma Endometrioide/tratamento farmacológico , Carcinoma Endometrioide/metabolismo , Cistadenocarcinoma Seroso/sangue , Cistadenocarcinoma Seroso/metabolismo , Desoxicitidina/administração & dosagem , Desoxicitidina/análogos & derivados , Intervalo Livre de Doença , Doxorrubicina/administração & dosagem , Resistencia a Medicamentos Antineoplásicos , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Etoposídeo/administração & dosagem , Feminino , Seguimentos , Humanos , Medições Luminescentes , Recidiva Local de Neoplasia , Neoplasias Ovarianas/sangue , Neoplasias Ovarianas/metabolismo , Paclitaxel/administração & dosagem , Valor Preditivo dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , Taxa de Sobrevida , Topotecan/administração & dosagem
16.
J Reprod Dev ; 56(1): 73-8, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19881221

RESUMO

Mammalian embryonic implantation requires reciprocal interactions between implantation-competent blastocysts and a receptive uterus. Some microRNAs might play a key role during embryo implantation in the mouse, but the let-7a expression profiles in the rat uterus during peri-implantation are unknown. In the study, the expression of let-7a in the uterus during early pregnancy, pseudopregnancy, artificial decidualization and activation of delayed implantation was detected by Northern blotting and in situ hybridization. The effect of steroid hormones on let-7a expression was also detected by Northern blotting and in situ hybridization. Here, we found that the expression level of let-7a was higher on gestation day 6-7 (g.d. 6-7) in rats than on g.d.4-5 and g.d.8-9. Let-7a was specifically localized in glandular and luminal epithelia and decidua. The expression of let-7a was not significantly different in the pseudopregnant uterus and increased significantly in the uteri of rats subjected to artificial decidualization and activation of delayed implantation. Treatment with estradiol-17beta or progesterone significantly increased let-7a expression. Thus, let-7a expression was significantly induced by the process of embryo invasion, and this increased expression level was mainly induced by active blastocysts and decidualization during the window of implantation, implying that let-7a may participate in endometrial decidualization. Steroid hormones, estradiol-17beta or progesterone stimulated let-7a expression.


Assuntos
Implantação do Embrião/fisiologia , MicroRNAs/metabolismo , Útero/metabolismo , Animais , Blastocisto/metabolismo , Blastocisto/fisiologia , Decídua/metabolismo , Decídua/fisiologia , Endométrio/metabolismo , Endométrio/fisiologia , Feminino , Hormônios Esteroides Gonadais/metabolismo , Hormônios Esteroides Gonadais/fisiologia , Gravidez , Pseudogravidez/fisiopatologia , Ratos , Ratos Sprague-Dawley
17.
Cell Physiol Biochem ; 23(4-6): 347-58, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19471102

RESUMO

BACKGROUND: MicroRNAs (miRNAs) are small noncoding RNAs whose function as modulators of gene expression is crucial for the proper control of cell growth. Although many microRNAs were found to express in central nervous system (CNS), the role of the regulatory networks in which they are involved and their function in the pathological process of nerve cells are only just emerging. In the present study, the possible mechanisms by which one neuronal miRNAs, miR-125b, affected the growth of nervous cells were investigated using in vitro cell line model. METHODS: The expression pattern of miR-125b in ATRA-treated human glioma cell lines was detected by Northern blotting and in situ localization. The effect of miR-125b on the proliferation and apoptosis of human glioma cells was analyzed by MTS assay, TUNEL and Flow cytometry analysis. In addition, the identification of target gene of miR-125b was studied by dual-luciferase activity assay and Immunoblot Analysis. RESULTS: We found differential expression of miR-125b in 1.0 microM all-trans-retinoic acid (ATRA)-treated human glioma cell lines. Up-regulation of miR-125b partially restored cell viability and inhibited cell apoptosis in U343 cells treated by ATRA. Down-regulation of miR-125b decreased human glioma cells proliferation and enhanced the sensitivity of human glioma cells to ATRA-induced apoptosis. In addition, we found an inverse relationship between the expression of miR-125b and the cell apoptosis-related protein Bcl-2 modifying factor (Bmf), and miR-125b can interact with 3'-untranslated region (UTR) of Bmf. CONCLUSION: These findings indicate that overexpression of miR-125b promotes human glioma cell proliferation and inhibits ATRA-induced cell apoptosis and low expression of miR-125b sensitizes cells to ATRA-induced apoptosis. BMF may play an important role in the process of miR-125b influencing cell apoptosis.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Apoptose , Proliferação de Células , Glioma/metabolismo , MicroRNAs/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Sequência de Bases , Regulação para Baixo , Glioma/genética , Humanos , MicroRNAs/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Tretinoína/farmacologia , Células Tumorais Cultivadas , Proteína X Associada a bcl-2/metabolismo
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