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1.
ACS Nano ; 14(3): 3696-3702, 2020 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-32150394

RESUMO

Light emission induced by chemical reactions, known as chemiluminescence (CL), has been widely used for bioassays, biosensors, imaging, and illumination applications. Most known CL systems exhibit flash-type single-color light emissions, which limit their applications. Long-lasting multicolor CL in aqueous solutions is highly desirable, especially for biological applications, but remains a challenge. Herein, we report a simple strategy of achieving highly efficient cascade Förster resonance energy transfer (FRET) in the dynamic nanoassembly of ß-cyclodextrin (ß-CD), CL reagents, and fluorophores in aqueous solution, which emits intensive multicolor CL with adjustable wavelength within 410-610 nm. ß-CD can bind CL reagents and fluorophores to form a dynamic nanoassembly. These nanoassemblies can bring the included luminescent intermediate and fluorophores into close proximity and proper alignment, which should greatly enhance the FRET efficiency between luminescent intermediate and fluorophores. Indeed, the cascade FRET efficiency in this supramolecular nanoassembly reaches up to 92%, which is comparable with the cascade FRET systems based on covalently linked donors and acceptors. By using hydroxypropyl methylcellulose as the thickener to slow the diffusion (to elongate the CL emission), and using Ca(OH)2 solid (a low solubility strong base) as buffer to maintain the pH in the optimal range for the CL reaction, this nanoassembly system has been further developed to achieve slow-diffusion-controlled catalytic CL reactions, which enables long-lasting multicolor CL in aqueous solution that is visible to naked eyes and lasts for more than 20 h. The multicolor CL systems can be used to prepare transformable two-dimensional multicolor codes for encryption application.

2.
ChemSusChem ; 12(16): 3808-3816, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31216377

RESUMO

Organic-inorganic halide perovskite solar cells (PSCs) have reached certified efficiencies of over 23 % with expensive organic hole-transporting materials. However, the use of an inorganic hole-transport layer (HTL) remains crucial as it would reduce cost combined with higher mobility and stability. In this direction, the application of Cu2 O as the top layer in PSCs is still complicated owing to the difficulty of solution processing. Herein, a solution-processing method is reported for preparing Cu2 O nanocubes as a p-type HTL in regular structure (n-i-p) PSCs. The controlled synthesis of Cu2 O nanocubes in a size range of 60-80 nm is achieved without using any surfactants, which are usually toxic and tricky to remove. The new structure of these Cu2 O nanocubes enhances the carrier mobility with preferable energy alignment to the perovskite layer and superb stability. The PSCs based on these Cu2 O nanocubes HTMs could achieve an efficiency exceeding 17 % with high stability, whereas organic P3HT-based PSCs display an efficiency of 15.59 % with a poorer running stability. This indicates that Cu2 O nanocubes are a promising HTM for efficient and stable PSCs.

4.
ACS Appl Mater Interfaces ; 11(24): 22021-22027, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31140268

RESUMO

Poly(3,4-ethylenedioxythiophene)/polystyrene sulfonate (PEDOT:PSS) plays an important role in inverted planar perovskite solar cells (IPPSCs) as an efficient hole extraction and transfer layer (HTL). The IPPSCs based on PEDOT:PSS normally display inferior performance with a reduced open-circuit voltage. To address this problem, here sodium citrate-doped PEDOT:PSS is adopted as an effective HTL for improving the performance of IPPSCs. Sodium citrate-doped PEDOT:PSS HTL improves the conversion efficiency of IPPSCs from 15.05% of reference cells to 18.39%. The large increase of the open-circuit voltage ( VOC) from 1.057 to 1.134 V is the main source for this performance enhancement. With the help of characterization analysis of ultraviolet photoelectron spectroscopy, scanning electron microscopy, electrochemical impedance spectroscopy, etc., the higher work function of the doped PEDOT:PSS film and the uniform crystallinity of the perovskite film on it are disclosed as the reasons for the increased VOC and the consequent performance enhancement.

5.
Public Health Nurs ; 36(3): 257-269, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30680796

RESUMO

BACKGROUND: Multidrug-resistant tuberculosis (MDR-TB) brings major challenges to the health care workers (HCWs). This study is to determine the risk factors for MDR-TB, latent tuberculosis infection (LTBI), and tuberculosis (TB) disease among HCWs in China. METHODS: A meta-analysis was conducted to evaluate the risk factors for MDR-TB, LTBI, and TB disease among HCWs using a random-effects model, and the pooled odds ratios (ORs) with 95% confidence interval (CI) were used as effect indicators. RESULTS: We identified 46 eligible studies and found eight factors were associated with MDR. The ORs with 95% CI are migrant population 1.96 (95% CI, 1.50-2.57), low family income 2.23 (95% CI, 1.74-2.85), retreatment 7.22 (95% CI, 5.63-9.26), anti-TB treatment history 5.65 (95% CI, 4.80-6.65), multiple episodes of treatment 3.28 (95% CI, 2.60-4.13), adverse reactions 3.48 (95% CI, 2.54-4.76), interrupted treatment 3.18 (95% CI, 2.60-3.89), and lung cavities 1.42 (95% CI, 1.14-1.77). Work duration as a HCW for 5 years and above increased the risk of LTBI and TB. HCWs aged 30 years and above were more susceptible to TB (OR = 1.70, 95% CI: 1.37-2.09). CONCLUSION: The risk factors for MDR-TB in China are possibly migrant population, low family income, retreatment, anti-TB treatment history, adverse reactions, interrupted treatment, and lung cavities. Longer work duration and greater age are risk factors for LTBI and TB among HCWs.


Assuntos
Pessoal de Saúde/estatística & dados numéricos , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia , China/epidemiologia , Humanos , Tuberculose Latente/epidemiologia , Fatores de Risco , Tuberculose/epidemiologia
6.
Laryngoscope ; 129(9): E318-E328, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-30597574

RESUMO

OBJECTIVES/HYPOTHESIS: Transformer-2 protein homolog beta (Tra2ß) generally plays an important role in various human cancers, but its role and the underlying mechanisms in laryngeal squamous cell carcinoma (LSCC) remained unknown. So this study aimed to assess the clinical significance and regulatory mechanisms of Tra2ß in LSCC. STUDY DESIGN: Laboratory analysis. METHODS: Expression of Tra2ß was compared in human LSCC tissue samples and paired adjacent normal tissue samples. The in vitro effects of Tra2ß expression in Hep-2 cells on their proliferation, invasion, and migration were assessed by CCK-8 assays, Matrigel invasion, and transwell migration assays. In addition, the effects of downregulation of Tra2ß on the activation of PI3K/AKT signaling pathway were measured using Western blot analysis. The effect of Tra2ß on the growth of tumors was detected in the Hep-2-injected xenograft models in vivo. RESULTS: Reverse-transcription quantitative polymerase chain reaction analysis and immunochemistry analysis indicated that the increased expression of Tra2ß in LSCC was significantly associated with poor differentiation, lymph node metastasis, and advanced clinical stage. In vitro knockdown of Tra2ß caused a significant decrease in the proliferation, invasion, and migration of Hep-2 cells. Tra2ß silencing decreased the expression of Bcl-2 but increased Bax and Caspase-3 both in mRNA and protein levels. Furthermore, knockdown of Tra2ß eliminated the suppressive effects of activation of PI3K/AKT signaling. In vivo knockdown of Tra2ß significantly inhibited the tumor growth of Hep-2-injected xenograft mice. CONCLUSIONS: The results of the present study demonstrated that knockdown of Tra2ß inhibits the proliferation and invasion of LSCC cells, at least partly via inhibiting PI3K/AKT signaling. LEVEL OF EVIDENCE: NA Laryngoscope, 129:E318-E328, 2019.


Assuntos
Carcinoma de Células Escamosas/genética , Inativação Gênica/fisiologia , Neoplasias Laríngeas/genética , Proteínas do Tecido Nervoso/metabolismo , Fatores de Processamento de Serina-Arginina/metabolismo , Transdução de Sinais/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Humanos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo
7.
J Org Chem ; 83(18): 10916-10921, 2018 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-30122034

RESUMO

We report a crystalline compound 2-azido-4,6-dimethoxy-1,3,5-triazine (ADT) as an intrinsically safe, highly efficient, and shelf-stable diazo-transfer reagent. Because the decomposition of ADT is an endothermal process (Δ H = 30.3 kJ mol-1), ADT is intrinsically nonexplosive, as proved by thermal, friction, and impact tests. The diazo-transfer reaction based on ADT gives diazo compounds in excellent yields within several minutes at room temperature. ADT is very stable upon >1 year storage under air at room temperature.

8.
J Appl Toxicol ; 38(11): 1437-1446, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30051583

RESUMO

Cadmium is considered one of the most harmful carcinogenic heavy metals in the human body. Although many scientists have performed research on cadmium toxicity mechanism, the toxicokinetic process of cadmium toxicity remains unclear. In the present study, the kinetic response of proteome in/and A549 cells to exposure of exogenous cadmium was profiled. A549 cells were treated with cadmium sulfate (CdSO4 ) for different periods and expressions of proteins in cells were detected by two-dimensional gel electrophoresis. The kinetic expressions of proteins related to cadmium toxicity were further investigated by reverse transcription-polymerase chain reaction and western blotting. Intracellular cadmium accumulation and content fluctuation of several essential metals were observed after 0-24 hours of exposure by inductively coupled plasma mass spectrometry. Fifty-four protein spots showed significantly differential responses to CdSO4 exposure at both 4.5 and 24 hours. From these proteins, four expression patterns were concluded. Their expressions always exhibited a maximum abundance ratio after CdSO4 exposure for 24 hours. The expression of metallothionein-1 and ZIP-8, concentration of total protein, and contents of cadmium, zinc, copper, cobalt and manganese in cells also showed regular change. In synthesis, the replacement of the essential metals, the inhibition of the expression of metal storing protein and the activation of metal efflux system are involved in cadmium toxicity.


Assuntos
Cádmio/toxicidade , Proteínas de Transporte de Cátions/metabolismo , Metalotioneína/metabolismo , Proteoma/metabolismo , Células A549 , Cádmio/metabolismo , Proteínas de Transporte de Cátions/genética , Técnicas de Cultura de Células , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Expressão Gênica/efeitos dos fármacos , Humanos , Metalotioneína/genética , Proteoma/genética , Fatores de Tempo , Toxicocinética
9.
Sci Total Environ ; 642: 1145-1152, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30045496

RESUMO

Simultaneous nitrification, denitrification and phosphorus removal (SNDPR) using aerobic granules is a promising approach in water treatment. The present work investigated the effects of influent carbon to nitrogen (N) ratios (20, 10, and 4) on the SNDPR performance in aerobic granular sequencing batch reactors (AGSBR) under high aeration rate. Results revealed that granules remained long-term stability when the DO concentration was 7-8 mg/L. With the decline of COD/N ratios, the denitrification efficiency was reduced due to the accumulation of nitrate, although the removal of COD and TP remained stable with good efficiency. Rising concentration of ammonia N led to the increase of PN/PS ratio of EPS as well as the protein types according to the results of 3D-EEM fluorescence spectroscopy. MiSeq pyrosequencing technology indicated that the decreasing ratio of COD/N under high DO concentration contributed to accumulation of GAOs and DNPAOs rather than PAOs.

10.
J Dent ; 74: 79-89, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29702152

RESUMO

OBJECTIVES: The water-associated attributes of resin-dentin interfaces created by contemporary adhesives are important determinants of bond integrity and stability. In the present work, these attributes were estimated from the perspectives of causality, to examine the behavior of the first and most-recently launched versions of universal adhesives when applied in either the etch-and-rinse mode or the self-etch mode. METHODS: The immediate cause of interfacial permeability and the time-dependent cause of water sorption were investigated in conjunction with the intermediate effect of interface degradation and the more long-term effect of loss of mechanical strength, before and after thermomechanical cycling. The results were compared with control etch-and-rinse and self-etch adhesives. RESULTS: Although the introduction of this new class of universal adhesives has brought forth significant changes to the dental adhesion arena, including more application options, reduced bonding armamentarium and increased user friendliness, the water-associated attributes that are critical for making resin-dentin bonds more durable to environmental challenges and less susceptible to degradation have remained unchanged at large, when compared with benchmarks established by former classes of adhesives. CONCLUSION: It appears that the current trend of adhesive development has brought forth significant changes but lacks the vigor that demarcates progress and technological sublimity. CLINICAL SIGNIFICANCE: The advent of the user friendly universal adhesives has brought forth significant changes to the dental adhesion arena. However, the elements that are critical for making resin-dentin bonds more durable to environmental challenges and less susceptible to degradation have remained unchanged at large.


Assuntos
Colagem Dentária/métodos , Adesivos Dentinários/química , Dentina/química , Cimentos de Resina/química , Água/química , Ataque Ácido Dentário/métodos , Resinas Compostas/química , Coroas , Esmalte Dentário , Materiais Dentários/química , Humanos , Teste de Materiais , Dente Serotino , Permeabilidade , Propriedades de Superfície , Resistência à Tração
11.
Phys Chem Chem Phys ; 20(12): 8064-8070, 2018 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-29513316

RESUMO

Many physical processes such as exciton interfacial dissociation, exciton interfacial recombination, and exciton-electron and exciton-hole interactions coexist at the interface of organic solar cells (OSC). In this study, the direction of free charge generation is defined as the direction from the interface to the side where free charges are left. For a p-n type device, the direction of free electron (hole) generation from exciton dissociation at the donor/accepter (D/A) interface is the same as the subsequent transportation direction under the built-in electric field. However, the direction of free electron (hole) generation from exciton-exciton recombination across the D/A interface is opposite to the direction of free charge transportation. Both free charges generated from exciton interfacial dissociation and recombination are contributed to the photocurrent for a p-n type device. In a device with a heterojunction formed by two n-type materials (here it is defined as an n-n type device), the direction of free electron (hole) generation from exciton recombination across the interface is also the same as the subsequent free charge transportation. At the same time, there are also some free electrons (free holes) generated by exciton interfacial dissociation. The direction of free charge generation from exciton dissociation for this n-n type device is also opposite to the direction of free charge transportation. However, only free charges generated from exciton interfacial recombination are contributed to the photocurrent for an n-n type device. But so far there has been no direct experimental evidence to prove the above theories. In this work, an NPB interfacial layer with a high LUMO was introduced in an n-n type OSC to inhibit the backflow of electrons, which are generated from exciton dissociation at the heterojunction formed by two n-type materials, enhancing the device performance accordingly. This work is conducive to interfacial engineering in an OSC to further improve its performance.

12.
Acta Biomater ; 67: 354-365, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29274477

RESUMO

Intrafibrillar silicified collagen scaffold (SCS) is a promising biomaterial for bone regeneration because it promotes cell homing and angiogenesis in bone defects via monocyte modulation. In the present study, a rat femoral defect model was used to examine the contribution of monocyte signaling pathways to SCS modulation. Activation of the monocyte p38 signaling pathway by SCS resulted in monocyte differentiation into TRAP-positive mononuclear cells. These cells demonstrated increased secretion of SDF-1α, VEGFa and PDGF-BB, which, in turn, promoted homing of bone marrow stromal cells (BMSCs) and endothelial progenitor cells (EPCs), as well as local vascularization. Monocyte differentiation and secretion were blocked after inhibition of the p38 pathway, which resulted in reduction in cell homing and angiogenesis. Taken together, these novel findings indicate that the p38 signaling pathway is crucial in SCS-modulated monocyte differentiation and secretion, which has a direct impact on SCS-induced bone regeneration. STATEMENT OF SIGNIFICANCE: Intrafibrillar silicified collagen scaffold (SCS) is a promising biomaterial for bone regeneration. The present work demonstrates that SCS possesses favorable bone regeneration potential in a rat femoral defect model. The degrading scaffold modulates monocyte differentiation and release of certain cytokines to recruit MSCs and EPCs, as well as enhances local vascularization by activating the p38 MAPK signaling pathway. These findings indicate that SCS contributes to bone defect regeneration by stimulating host cell homing and promoting local angiogenesis and osteogenesis without the need for loading cytokines or xenogenous stem cells.


Assuntos
Regeneração Óssea/fisiologia , Colágenos Fibrilares/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Monócitos/enzimologia , Dióxido de Silício/química , Tecidos Suporte/química , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Regeneração Óssea/efeitos dos fármacos , Citocinas/metabolismo , Modelos Animais de Doenças , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Fêmur/patologia , Masculino , Camundongos Endogâmicos C57BL , Monócitos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley
13.
Pak J Pharm Sci ; 30(5(Special)): 1829-1832, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29084653

RESUMO

The aim of present research work is study the effect of levofloxacononone chalcone derivatives on apoptosis and autophagy of HCC SMMC-7721 cells in patients with hepatocellular carcinoma (HCC). The HCC SMMC-7721 cells in the logarithmic phase growth were inoculated, and the proliferation of SMMC -7721 cells was detected by MTT assay. The effect of levofloxacononone chalcone derivatives on SMMC-7721 cell cycle and apoptosis rate was detected by flow cytometry. Cells were treated by autophagy inhibitor chloroquine to validate the effect of levofloxacononone chalcone derivatives on cell proliferation and apoptosis. Levofloxacononone chalcone derivatives could significantly inhibit the proliferation of SMMC-7721 cells. Compared with the control group, the apoptosis rate of cells treated with levofloxacononone chalcone derivatives was increased significantly in 24h, showing significant difference between groups. Chloroquine could increase the inhibitory effect of low-dose levofloxacononone chalcone derivatives on SMMC-7721 cell proliferation, and decrease the inhibitory effect of high-dose levofloxacononone chalcone derivatives on SMMC-7721 cell proliferation. Levofloxacononone chalcone derivatives can obviously induce the apoptosis and autophagy of SMMC-7721 cells, at a low dose, its autophagy can protect cells; and at a high dose, the autophagy can decrease the cell proliferation rate, to promote cell apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Chalcona/análogos & derivados , Chalcona/farmacologia , Chalconas/farmacologia , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Chalcona/administração & dosagem , Cloroquina/farmacologia , Relação Dose-Resposta a Droga , Humanos
14.
Bioresour Technol ; 241: 127-133, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28551433

RESUMO

An aerobic granules simultaneous nitrification, denitrification and phosphorus removal (SNDPR) system was evaluated in terms of the reactor performance and microbial population dynamics with decreasing C/P ratios from 50 to 16. The effects of C/P ratios on organic carbon and nutrients removal were investigated, as well as the alpha diversity of the bacterial community and bacterial compositions by using Illumina MiSeq pyrosequencing technology. Finally, the relative abundances and distribution patterns were identified and assessed given the key functional groups involved in biological nutrients removals to reveal the effects of C/P ratios to aerobic granules in the SNDPR from the molecular level.


Assuntos
Reatores Biológicos , Desnitrificação , Fósforo , Carbono , Nitrificação , Nitrogênio , Esgotos
15.
Sci Rep ; 7: 44058, 2017 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-28276451

RESUMO

Stem cells therapy has been suggested as a promising option for the treatment of acute kidney injury (AKI). This study was performed to compare the abilities of xenogenic transplantation of human adipose stromal vascular fraction (SVF) and adipose-derived mesenchymal stem cells (AdMSCs) to facilitate the recovery of renal function and structure in a rat model of ischemia/reperfusion (IR) induced AKI. SVF or AdMSCs were transplanted to the injured kidney through intra-parenchymal injection. Significantly improved renal function and reduced tubular injury were observed in SVF and AdMSCs groups. Administration of SVF or AdMSCs contributed to significantly improved cell proliferation and markedly reduced cell apoptosis in parallel with reduced microvascular rarefaction in injured kidney. IR injury resulted in higher levels of inflammatory cytokines, whereas xenogenic transplantation of SVF or AdMSCs reduced but not induced inflammatory cytokines expression. Additionally, in vitro study showed that administration of SVF or AdMSCs could also significantly promote the proliferation and survival of renal tubular epithelial cells underwent hypoxia/reoxygenation injury through secreting various growth factors. However, cell proliferation was significantly promoted in SVF group than in AdMSCs group. In conclusion, our study demonstrated that administration of SVF or AdMSCs was equally effective in attenuating acute renal IR injury.


Assuntos
Tecido Adiposo/metabolismo , Nefropatias/terapia , Rim/metabolismo , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Traumatismo por Reperfusão/terapia , Tecido Adiposo/patologia , Adulto , Animais , Feminino , Xenoenxertos , Humanos , Rim/patologia , Nefropatias/metabolismo , Nefropatias/patologia , Masculino , Células-Tronco Mesenquimais/patologia , Pessoa de Meia-Idade , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia
16.
Biomaterials ; 113: 203-216, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27821306

RESUMO

The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotes in situ bone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-ß1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.


Assuntos
Regeneração Óssea , Colágeno/química , Monócitos/citologia , Neovascularização Fisiológica , Ácido Silícico/química , Crânio/fisiologia , Tecidos Suporte/química , Animais , Células Cultivadas , Quimiotaxia , Colágeno/imunologia , Imunomodulação , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/imunologia , Camundongos Endogâmicos C57BL , Monócitos/imunologia , Ácido Silícico/imunologia , Crânio/irrigação sanguínea , Crânio/imunologia , Crânio/lesões
17.
Zhonghua Nan Ke Xue ; 23(3): 217-222, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29706041

RESUMO

Objective: To compare the clinical effect of diode laser enucleation of the prostate (DIOD) with that of transurethral resection of the prostate (TURP) on benign prostate hyperplasia (BPH) with different prostate volumes. METHODS: This retrospective study included 256 BPH patients treated by DIOD (n = 141) or TURP (n = 115) from March 2012 to August 2015. According to the prostate volume, we divided the patients into three groups: <60 ml (42 for DIOD and 31 for TURP), 60-80 ml (51 for DIOD and 45 for TURP), and >80 ml (48 for DIOD and 39 for TURP). We obtained the relevant data from the patients before, during and at 6 months after surgery, and compared the two surgical strategies in operation time, perioperative levels of hemoglobin and sodium ion, post-operative urethral catheterization time and bladder irrigation time, pre- and post-operative serum PSA levels, International Prostate Symptoms Score (IPSS), post-void residual urine (PVR) volume and maximum urinary flow rate (Qmax), and incidence of post-operative complications among different groups. RESULTS: In the <60 ml group, there were no remarkable differences in the peri- and post-operative parameters between the two surgical strategies. In the 60-80 ml group, DIOD exhibited a significant superiority over TURP in the perioperative levels of hemoglobin (ï¼»3.25 ± 1.53ï¼½ g/L vs ï¼»4.77 ± 1.67ï¼½ g/L, P <0.05) and Na+ (ï¼»3.58 ± 1.27ï¼½mmol/L vs ï¼»9.67 ± 2.67ï¼½ mmol/L, P <0.01), bladder irrigation time (ï¼»30.06 ± 6.22ï¼½h vs ï¼»58.32 ± 10.25ï¼½ h, P <0.01), and urethral catheterization time (ï¼»47.61 ± 13.55ï¼½ h vs ï¼»68.01 ± 9.69ï¼½ h, P <0.01), but a more significant decline than the latter in the postoperative PSA level (ï¼»2.34 ± 1.29ï¼½ ng/ml vs ï¼»1.09 ± 0.72ï¼½ ng/ml, P <0.05), and similar decline was also seen in the >80 ml group (ï¼»3.35 ± 1.39ï¼½ ng/ml vs ï¼»1.76 ± 0.91ï¼½ ng/ml, P <0.05). No blood transfusion was necessitated and nor postoperative transurethral resection syndrome or urethral stricture observed in DIOD. However, the incidence rate of postoperative pseudo-urinary incontinence was significantly higher in the DIOD (22.7%, 32/141) than in the TURP group (7.83%, 9/115) (P <0.05). CONCLUSIONS: DIOD, with its obvious advantages of less blood loss, higher safety, faster recovery, and more definite short-term effectiveness, is better than TURP in the treatment of BPH with medium or large prostate volume and similar to the latter with small prostate volume.


Assuntos
Lasers Semicondutores/uso terapêutico , Hiperplasia Prostática/cirurgia , Ressecção Transuretral da Próstata/métodos , Humanos , Lasers Semicondutores/efeitos adversos , Masculino , Duração da Cirurgia , Tamanho do Órgão , Complicações Pós-Operatórias/etiologia , Próstata/patologia , Hiperplasia Prostática/patologia , Qualidade de Vida , Estudos Retrospectivos , Irrigação Terapêutica , Ressecção Transuretral da Próstata/efeitos adversos , Ressecção Transuretral da Próstata/estatística & dados numéricos , Resultado do Tratamento , Estreitamento Uretral/etiologia , Cateterismo Urinário , Incontinência Urinária/etiologia
18.
Stem Cells Transl Med ; 5(9): 1277-88, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27365485

RESUMO

UNLABELLED: : Ischemia/reperfusion (IR)-induced acute kidney injury (AKI) is a common clinical syndrome. Stem/progenitor cell therapy is a promising option to foster the intrinsic capacity for kidney regeneration. However, there are still several challenges to be resolved, including the potential risks during cell culture, low retention rate after transplantation, and unclear effect on the progression of chronic kidney disease (CKD). Recently, nonexpanded adipose stromal vascular fraction (SVF) has been regarded as an attractive cell source for cell-based therapy. Preconditioning with ischemia has been suggested as a useful method to promote the retention and survival of transplanted cells in vivo. In this study, freshly isolated autologous SVF was transplanted to the kidney of rats before ischemia, and then an IR-induced AKI model was established. Postischemic administration of SVF to the kidney was performed after renal IR injury was induced. A higher cell retention rate was detected in the preischemic group. Preischemic administration of SVF showed stronger functional and morphologic protection from renal IR injury than postischemic administration, through enhancing tubular cell proliferation and reducing apoptosis. Progression of kidney fibrosis was also significantly delayed by preischemic administration of SVF, which exhibited stronger inhibition of transforming growth factor-ß1-induced epithelia-mesenchymal transition and microvascular rarefaction. In addition, in vitro study showed that prehypoxic administration of SVF could significantly promote the proliferation, migration, and survival of hypoxic renal tubular epithelial cells. In conclusion, our study demonstrated that preischemic administration of nonexpanded adipose SVF protected the kidney from both acute IR injury and long-term risk of developing CKD. SIGNIFICANCE: Renal ischemia/reperfusion (IR) injury is a common clinical syndrome. Cell-based therapy provides a promising option to promote renal repair after IR injury. However, several challenges still remain because of the potential risks during cell culture, low retention rate after transplantation, and unclear effect on the progression of chronic kidney disease. Stromal vascular fraction (SVF) is considered as an attractive cell source. This study demonstrated that preischemic administration of uncultured SVF could increase cell retention and then improve renal function and structure at both early and long-term stage after IR, which may provide a novel therapeutic approach for IR injury.


Assuntos
Tecido Adiposo/irrigação sanguínea , Movimento Celular , Proliferação de Células , Nefropatias , Túbulos Renais/metabolismo , Traumatismo por Reperfusão , Animais , Nefropatias/metabolismo , Nefropatias/patologia , Nefropatias/prevenção & controle , Túbulos Renais/patologia , Masculino , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Traumatismo por Reperfusão/prevenção & controle , Células Estromais/metabolismo , Células Estromais/patologia , Células Estromais/transplante
19.
Asian Pac J Cancer Prev ; 17(4): 2217-21, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27221921

RESUMO

Vascular endothelial growth factor 2 (VEGFR2) was initially identified as a receptor of VEGF on endothelial cells with a role in regulating angiogenesis during organism development and tumorigenesis. Previously, in cancer tissue, VEGFR2 has been reported to be expressed in endothelial cells. In our research, we found that VEGFR2 was expressed not only in endothelial cells but also cancer cells in head and neck squamous cell carcinomas (HNSCCs). Knockdown of VEGFR2 in Hep2 cells could arrest the cell cycle in G0/G1, leading to a decrease in proliferation. We also present evidence that MAPK/ERK signal pathways and expression of CDK1 downstream of VEGFR2 might regulate proliferation and cell cycle arrest. Furthermore, we discovered that down-regulate VEGRF2 in Hep2 cells could significantly affect the invasion ability. Taken together, our data suggest that VEGFR2 might regulate proliferation and invasion in HNSCC cancer cells in vivo.


Assuntos
Carcinoma de Células Escamosas/patologia , Movimento Celular , Proliferação de Células , Neoplasias de Cabeça e Pescoço/patologia , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Apoptose , Western Blotting , Carcinoma de Células Escamosas/metabolismo , Ciclo Celular , Neoplasias de Cabeça e Pescoço/metabolismo , Humanos , Técnicas Imunoenzimáticas , Invasividade Neoplásica , Células Tumorais Cultivadas
20.
PLoS One ; 10(7): e0133451, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26196515

RESUMO

Zinc, an essential trace element, is involved in many important physiological processes. Cell responses to zinc stress show time-dependent effects besides concentration-dependence and tissue-specificity. Herein, we investigated the time-dependent differential expression of the proteome in A549 cells after administered with ZnSO4 for both 9 and 24 h using 2DE. 123 differentially expressed protein spots were detected, most of which were up-regulated by Zn2+ treatment. Interestingly, 49 proteins exhibited significant differential expression repeatedly during these two treatment periods, and moreover showed a conserved change with different ratios and four time-dependent expression patterns. Pattern 1 (up-regulated with rapid initial induction and subsequent repression) and pattern 4 (down-regulated with steady repression) were the predominant expression patterns. The abundances of the proteins in patterns 1 and 4 after 24 h of zinc treatment are always lower than that after 9 h, indicating that exogenous zinc reduced the expression of proteins in cells after 24 h or longer. Importantly, these findings could also reflect the central challenge in detecting zinc homeostasis proteins by 2DE or other high throughput analytical methods resulting from slight variation in protein expression after certain durations of exogenous zinc treatment and/or low inherent protein content in cells. These time-dependent proteome expression patterns were further validated by measuring dynamic changes in protein content in cells and in expression of two proteins using the Bradford method and western blotting, respectively. The time-dependent changes in total zinc and free Zn2+ ion contents in cells were measured using ICP-MS and confocal microscopy, respectively. The kinetic process of zinc homeostasis regulated by muffling was further revealed. In addition, we identified 50 differentially expressed proteins which are predominantly involved in metabolic process, cellular process or developmental process, and function as binding, catalytic activity or structural molecule activity. This study further elucidates our understanding of dynamic nature of the cellular response to zinc stress and the mechanism of zinc homeostasis.


Assuntos
Proteoma/análise , Proteômica/métodos , Estresse Fisiológico , Sulfato de Zinco/farmacologia , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Western Blotting , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Regulação para Baixo/efeitos dos fármacos , Eletroforese em Gel Bidimensional , Humanos , Cinética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Espectrometria de Massas/métodos , Microscopia Confocal , Proteoma/classificação , Proteoma/metabolismo , Fatores de Tempo , Regulação para Cima/efeitos dos fármacos
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