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1.
Med Phys ; 2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34719042

RESUMO

PURPOSE: Cerebrovascular segmentation in magnetic resonance imaging (MRI) plays an important role in the diagnosis and treatment of cerebrovascular diseases. Many segmentation frameworks based on convolutional neural networks (CNNs) or U-Net-like structures have been proposed for cerebrovascular segmentation. Unfortunately, the segmentation results are still unsatisfactory, particularly in the small/thin cerebrovascular due to the following reasons: (1) the lack of attention to multiscale features in encoder caused by the convolutions with single kernel size; (2) insufficient extraction of shallow and deep-seated features caused by the depth limitation of transmission path between encoder and decoder; (3) insufficient utilization of the extracted features in decoder caused by less attention to multiscale features. METHODS: Inspired by U-Net++, we propose a novel 3D U-Net-like framework termed Usception for small cerebrovascular. It includes three blocks: Reduction block, Gap block, and Deep block, aiming to: (1) improve feature extraction ability by grouping different convolution sizes; (2) increase the number of multiscale features in different layers by grouping paths of different depths between encoder and decoder; (3) maximize the ability of decoder in recovering multiscale features from Reduction and Gap block by using convolutions with different kernel sizes. RESULTS: The proposed framework is evaluated on three public and in-house clinical magnetic resonance angiography (MRA) data sets. The experimental results show that our framework reaches an average dice score of 69.29%, 87.40%, 77.77% on three data sets, which outperform existing state-of-the-art methods. We also validate the effectiveness of each block through ablation experiments. CONCLUSIONS: By means of the combination of Inception-ResNet and dimension-expanded U-Net++, the proposed framework has demonstrated its capability to maximize multiscale feature extraction, thus achieving competitive segmentation results for small cerebrovascular.

2.
FEBS Open Bio ; 11(11): 3051-3062, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34510826

RESUMO

Human papillomavirus type 18 (HPV18) has high carcinogenic power in invasive cervical cancer (ICC) development. However, the underlying mechanism remains elusive. The carcinogenic properties of HPV18 require the PDZ-binding motif of its E6 oncoprotein (HPV18 E6) to degrade its target PSD95/Dlg/ZO-1 (PDZ) proteins. In this study, we demonstrated that the PDZ protein membrane-associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3) inhibited the Wnt/ß-catenin pathway, and subsequently cervical cancer (CC) cell migration and invasion, via decreasing ß-catenin levels. By reducing MAGI3 protein levels, HPV18 E6 promoted CC cell migration and invasion through activation of Wnt/ß-catenin signaling. Furthermore, HPV18 rather than HPV16 was preferentially associated with the downregulation of MAGI3 and activation of the Wnt/ß-catenin pathway in CC. These findings shed light on the mechanism that gives HPV18 its high carcinogenic potential in CC progression.

3.
IEEE Trans Image Process ; 30: 7677-7688, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34449357

RESUMO

Pose-based person image synthesis aims to generate a new image containing a person with a target pose conditioned on a source image containing a person with a specified pose. It is challenging as the target pose is arbitrary and often significantly differs from the specified source pose, which leads to large appearance discrepancy between the source and the target images. This paper presents the Pose Transform Generative Adversarial Network (PoT-GAN) for person image synthesis where the generator explicitly learns the transform between the two poses by manipulating the corresponding multi-scale feature maps. By incorporating the learned pose transform information into the multi-scale feature maps of the source image in a GAN architecture, our method reliably transfers the appearance of the person in the source image to the target pose with no need for any hard-coded spatial information depicting the change of pose. According to both qualitative and quantitative results, the proposed PoT-GAN demonstrates a state-of-the-art performance on three publicly available datasets for person image synthesis.

4.
Org Lett ; 23(15): 5998-6003, 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34237938

RESUMO

Herein we report binary acid Sc(OTf)3/TfOH-catalyzed alkenylation of arenes with alkynes. In this system, the high-energy vinyl carbocations with activated and weakly coordinating trifluoromethanesulfonate anions by Lewis acid Sc(III) can undergo facile Friedel-Crafts reactions with arenes to give the desired adducts in up to 90% yield and with high Z-selectivity.

5.
Neuroreport ; 32(12): 1001-1008, 2021 08 11.
Artigo em Inglês | MEDLINE | ID: mdl-34102645

RESUMO

OBJECTIVE: Ferroptosis plays an important role in traumatic brain injury (TBI). The p53 protein is a major mediator of ferroptosis. However, the role of p53-mediated ferroptosis in TBI has not been studied. Sirtuin 2 (SIRT2) exerts a protective effects role in TBI, although the underlying mechanism of this protection remains unclear. In the present study, we tested the hypothesis that that SIRT2 mitigates TBI by regulating p53-mediated ferroptosis. METHODS AND RESULTS: To model TBI in mice, we used the controlled cortical impact (CCI) injury method. We found that ferroptosis was significantly activated by CCI, and peaked 3 days following CCI, as evidenced by upregulation of GPX4 and SLC7A11, increased content of decreases glutathione, lipid peroxidation, malondialdehyde and ferrous ion. Inhibition of ferroptosis significantly alleviated neurological indications and brain edema. In addition, knockout of p53 significantly blocked ferroptosis following CCI. Furthermore, we found that inhibition of SIRT2 upregulated the acetylation of p53, as well as p53 expression, and exacerbated ferroptosis following CCI. Interestingly, knockout of p53 rescued the SIRT2 inhibition-induced exacerbation of ferroptosis. CONCLUSIONS: These findings indicate that p53-mediated ferroptosis contributes to the pathogenesis of TBI. Furthermore, we demonstrate that SIRT2 exerts a neuroprotective effect against TBI by suppressing p53-mediated ferroptosis.

6.
Dokl Biochem Biophys ; 497(1): 144-150, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33895931

RESUMO

Ovarian cancer is the malignant tumour of the female reproductive organ with highest mortality rate among all the types of gynaecological tumours. This study investigated the effect of Dioscorea deltoidea leaf extract (DDLE) on OV-90 and CAOV4 ovarian cancer cells. The results demonstrated that DDLE suppresses OV-90 and CAOV3 cell viability significantly in dose dependent manner. The OV-90 and CAOV3 cell viability were reduced to 24 and 27% respectively with 20 mg/mL DDLE treatment. Five mg/mL DDLE treatment of OV-90 and CAOV4 cells raised percentage of cells in G2-phase to 55.9 and 51.2%, respectively. In 5 mg/mL DDLE -treated OV-90 and CAOV4 cells a prominent suppression in cyclin-D1 and cyclin B1 proteins was observed in 48 h. The DDLE treatment promoted OV-90 and CAOV3 cell apoptosis to 34.65 and 29.89%, respectively. The Fas, FasL, cleaved caspase-3, and Bax levels were up-regulated markedly in the cells after DDLE treatment. Moreover, DDLE treatment suppressed p-mTOR, p-AKT and p-PI3K expression in OV-90 and CAOV3 cells. Thus, DDLE suppressed ovary cancer cell viability and elevated cell apoptosis. Inhibitory effect of DDLE on ovarian cancer cells is associated with targeting PI3K/AKT/mTOR pathway.


Assuntos
Dioscorea/química , Neoplasias Ovarianas/patologia , Fosfatidilinositol 3-Quinases/metabolismo , Extratos Vegetais/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Feminino , Humanos , Folhas de Planta/química , Regulação para Cima/efeitos dos fármacos
7.
PLoS One ; 16(4): e0249578, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33861786

RESUMO

INTRODUCTION AND OBJECTIVE: Ankylosing spondylitis (AS) has characteristics of spinal bone bridge and fusion. Although BMD reduction in AS may be presumed to be due to spinal inflammation, this study was designed to confirm whether immobilization of the spine due to syndesmophytes is related to BMD reduction, as immobilization itself is a risk factor for BMD reduction. METHODS: Among male patients diagnosed with AS according to the modified New York criteria, those who underwent bone density tests with quantitative computed tomography (QCT) were retrospectively analyzed through a chart review. The correlation between the presence or absence of bone bridges for each vertebral body level of the L spine confirmed with radiography and BMD confirmed with QCT was analyzed. RESULTS: A total of 47 male patients with AS were enrolled. The mean patient age was 46.8 ± 8.2 years, and the mean disease duration was 7.9 ± 6.4 years. The trabecular BMD of the lumbar spine (L1-L4) ranged from 23.1 to 158.45 mg/cm3 (mean 102.2 ± 37 mg/cm3), as measured with QCT. The lumbar BMD measurements showed that 30 patients (63.8%) had osteopenia or osteoporosis. Bone bridge formation showed a negative correlation with BMD. Low BMD was significantly correlated with bone bridge in the vertebral body (p < 0.05). Positive correlations were observed between bone bridge score and BASMI flexion score, whereas significant negative correlations were found between BMD and BASMI flexion score (p < 0.05). CONCLUSION: Decreased mobility of the vertebrae due to bone bridge formation affects the decrease in BMD in patients with AS.


Assuntos
Densidade Óssea , Doenças Ósseas Metabólicas/patologia , Lâmina de Crescimento/patologia , Vértebras Lombares/patologia , Fraturas da Coluna Vertebral/patologia , Espondilite Anquilosante/complicações , Tomografia Computadorizada por Raios X/métodos , Adulto , Doenças Ósseas Metabólicas/diagnóstico por imagem , Doenças Ósseas Metabólicas/etiologia , Lâmina de Crescimento/diagnóstico por imagem , Humanos , Vértebras Lombares/diagnóstico por imagem , Vértebras Lombares/lesões , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fraturas da Coluna Vertebral/diagnóstico por imagem
8.
Cell Rep ; 34(12): 108891, 2021 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-33761354

RESUMO

Myeloid lineage cells use TLRs to recognize and respond to diverse microbial ligands. Although unique transcription factors dictate the outcome of specific TLR signaling, whether lineage-specific differences exist to further modulate the quality of TLR-induced inflammation remains unclear. Comprehensive analysis of global gene transcription in human monocytes, monocyte-derived macrophages, and monocyte-derived dendritic cells stimulated with various TLR ligands identifies multiple lineage-specific, TLR-responsive gene programs. Monocytes are hyperresponsive to TLR7/8 stimulation that correlates with the higher expression of the receptors. While macrophages and monocytes express similar levels of TLR4, macrophages, but not monocytes, upregulate interferon-stimulated genes (ISGs) in response to TLR4 stimulation. We find that TLR4 signaling in macrophages uniquely engages transcription factor IRF1, which facilitates the opening of ISG loci for transcription. This study provides a critical mechanistic basis for lineage-specific TLR responses and uncovers IRF1 as a master regulator for the ISG transcriptional program in human macrophages.

9.
Medicine (Baltimore) ; 100(3): e24112, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33546019

RESUMO

BACKGROUND: Stroke is the main cause of death and disability in the world and insomnia is a common complication of stroke patients. Insomnia will not only seriously affect the prognosis and quality of life of patients with stroke, but even cause the recurrence of stroke. Many studies have proved that acupuncture and moxibustion can effectively improve insomnia symptoms. This study will systematically evaluate the effectiveness and safety of acupuncture combined with moxibustion in treating insomnia after stroke. METHODS: The following 8 databases will be searched from the inception to October 31, 2020, including China National Knowledge Infrastructure Database (CNKI), Chinese Scientific Journal Database (VIP database), China Biomedical Literature Database (CBM), Wanfang Data Chinese Database, PubMed, the Cochrane Central Register of Controlled Trials (CENTRAL), Allied and Alternative Medicine Database (AMED), Excerpt Medica Database (Embase). We will also search for ongoing trials from the World Health Organization International Clinical Trial Registration Platform search portal, Chinese Clinical Trial Register, Clinical trials.gov. In addition, the reference lists of studies meeting the inclusion criteria will also be searched for achieving the comprehensive retrieval to the maximum. All randomized controlled trials of acupuncture and moxibustion in treating insomnia after stroke will be included. Two reviewers will conduct literature screening, data extraction, and quality evaluation respectively. The main outcome is the Pittsburgh sleep quality index (PSQI), and the secondary outcomes include clinical efficacy, quality of life, and safety. RevMan V.5.4.1 will be used for meta-analysis. We will express the results as risk ratio (RR) with 95% confidence intervals (CIs) for dichotomous data and mean difference (MD) or standard mean difference (SMD) 95% CIs for continuous data. RESULTS: This study will provide a comprehensive review of the available evidence of acupuncture combined with moxibustion in treating insomnia after stroke. CONCLUSION: The conclusion of our study will provide the updated evidence to judge the effectiveness and safety of acupuncture combined with moxibustion for the treatment of insomnia after stroke. TRIAL REGISTRATION NUMBER: PROSPERO CRD42020216720.


Assuntos
Moxibustão , Distúrbios do Início e da Manutenção do Sono/terapia , Acidente Vascular Cerebral/complicações , Humanos , Metanálise como Assunto , Distúrbios do Início e da Manutenção do Sono/etiologia , Revisões Sistemáticas como Assunto
10.
Proc Natl Acad Sci U S A ; 118(7)2021 02 16.
Artigo em Inglês | MEDLINE | ID: mdl-33568531

RESUMO

Recent studies have identified thousands of long noncoding RNAs (lncRNAs) in mammalian genomes that regulate gene expression in different biological processes. Although lncRNAs have been identified in a variety of immune cells and implicated in immune response, the biological function and mechanism of the majority remain unexplored, especially in sepsis. Here, we identify a role for a lncRNA-gastric adenocarcinoma predictive long intergenic noncoding RNA (GAPLINC)-previously characterized for its role in cancer, now in the context of innate immunity, macrophages, and LPS-induced endotoxic shock. Transcriptome analysis of macrophages from humans and mice reveals that GAPLINC is a conserved lncRNA that is highly expressed following macrophage differentiation. Upon inflammatory activation, GAPLINC is rapidly down-regulated. Macrophages depleted of GAPLINC display enhanced expression of inflammatory genes at baseline, while overexpression of GAPLINC suppresses this response. Consistent with GAPLINC-depleted cells, Gaplinc knockout mice display enhanced basal levels of inflammatory genes and show resistance to LPS-induced endotoxic shock. Mechanistically, survival is linked to increased levels of nuclear NF-κB in Gaplinc knockout mice that drives basal expression of target genes typically only activated following inflammatory stimulation. We show that this activation of immune response genes prior to LPS challenge leads to decreased blood clot formation, which protects Gaplinc knockout mice from multiorgan failure and death. Together, our results identify a previously unknown function for GAPLINC as a negative regulator of inflammation and uncover a key role for this lncRNA in modulating endotoxic shock.


Assuntos
Imunidade Inata , Choque Séptico/imunologia , Animais , Células Cultivadas , Feminino , Humanos , Lipopolissacarídeos/toxicidade , Masculino , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Choque Séptico/etiologia , Choque Séptico/genética , Células THP-1 , Transcriptoma
11.
Oral Dis ; 27(6): 1443-1450, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33012087

RESUMO

OBJECTIVE: To estimate the risk of developing autoimmune disease in patients diagnosed having recurrent aphthous stomatitis (RAS) through a nationwide population-based cohort study. METHODS: This study included two group of patients who had three or more episodes with aphthae diagnosed from their physician (RAS group) and a similar matched group of patients without aphthae (control group). Both groups were collected within the period of 2005-2007 from the Korean National Health Insurances claims database. Non-RAS cohort was matched after frequency matching. The final enrolled subjects were observed during a follow-up period from 2008 to 2015 and those who received autoimmune diseases diagnoses during follow-up were identified. The hazard ratio (HR) for developing autoimmune diseases was estimated. RESULTS: A total of 4,637 patients with RAS and 4,637 controls were included. The risk of overall autoimmune diseases was significantly increased in the RAS group (adjusted HR [aHR)], 1.19). With regard to each disease entity, patients with RAS showed an increased risk of Behcet's disease (31.16), systemic lupus erythematous (SLE) (1.74), ankylosing spondylitis (AS) (1.47), gout (1.47), Hashimoto thyroiditis (1.42), Graves' disease (1.37), and rheumatoid arthritis (RA) (1.19). CONCLUSION: RAS-like lesion may be an early sign of systemic autoimmune disease, as it was associated with an increased risk of Graves' disease, Hashimoto thyroiditis, SLE, AS, gout, RA, and Behcet's disease from real-world data.


Assuntos
Doenças Autoimunes , Síndrome de Behçet , Estomatite Aftosa , Doenças Autoimunes/complicações , Doenças Autoimunes/epidemiologia , Estudos de Coortes , Humanos , Projetos de Pesquisa , Estomatite Aftosa/epidemiologia , Estomatite Aftosa/etiologia
12.
IEEE Trans Vis Comput Graph ; 27(1): 151-164, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31329121

RESUMO

Recently, effort has been made to apply deep learning to the detection of mesh saliency. However, one major barrier is to collect a large amount of vertex-level annotation as saliency ground truth for training the neural networks. Quite a few pilot studies showed that this task is difficult. In this work, we solve this problem by developing a novel network trained in a weakly supervised manner. The training is end-to-end and does not require any saliency ground truth but only the class membership of meshes. Our Classification-for-Saliency CNN (CfS-CNN) employs a multi-view setup and contains a newly designed two-channel structure which integrates view-based features of both classification and saliency. It essentially transfers knowledge from 3D object classification to mesh saliency. Our approach significantly outperforms the existing state-of-the-art methods according to extensive experimental results. Also, the CfS-CNN can be directly used for scene saliency. We showcase two novel applications based on scene saliency to demonstrate its utility.

13.
Org Lett ; 22(22): 8941-8946, 2020 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-33166144

RESUMO

A bioinspired radical oxidative α-oxyamination of pyruvate with an oxoammonium salt through multiple-site concerted proton-electron transfer process has been developed, which was facilitated by anchoring the mercapoto chains as a "hopping" site at the γ-position of α-keto esters.

14.
Genome Biol ; 21(1): 281, 2020 11 19.
Artigo em Inglês | MEDLINE | ID: mdl-33213505

RESUMO

BACKGROUND: Systemic lupus erythematosus (SLE) is a clinically heterogeneous autoimmune disease characterized by the development of anti-nuclear antibodies. Susceptibility to SLE is multifactorial, with a combination of genetic and environmental risk factors contributing to disease development. Like other polygenic diseases, a significant proportion of estimated SLE heritability is not accounted for by common disease alleles analyzed by SNP array-based GWASs. Death-associated protein 1 (DAP1) was implicated as a candidate gene in a previous familial linkage study of SLE and rheumatoid arthritis, but the association has not been explored further. RESULTS: We perform deep sequencing across the DAP1 genomic segment in 2032 SLE patients, and healthy controls, and discover a low-frequency functional haplotype strongly associated with SLE risk in multiple ethnicities. We find multiple cis-eQTLs embedded in a risk haplotype that progressively downregulates DAP1 transcription in immune cells. Decreased DAP1 transcription results in reduced DAP1 protein in peripheral blood mononuclear cells, monocytes, and lymphoblastoid cell lines, leading to enhanced autophagic flux in immune cells expressing the DAP1 risk haplotype. Patients with DAP1 risk allele exhibit significantly higher autoantibody titers and altered expression of the immune system, autophagy, and apoptosis pathway transcripts, indicating that the DAP1 risk allele mediates enhanced autophagy, leading to the survival of autoreactive lymphocytes and increased autoantibody. CONCLUSIONS: We demonstrate how targeted sequencing captures low-frequency functional risk alleles that are missed by SNP array-based studies. SLE patients with the DAP1 genotype have distinct autoantibody and transcription profiles, supporting the dissection of SLE heterogeneity by genetic analysis.

15.
J Cancer ; 11(20): 6114-6121, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32922551

RESUMO

Background: This study aimed to develop a predictive model based on the risk of locoregional recurrence (LRR) in epidermal growth factor receptor (EGFR)-mutant stage III-pN2 lung adenocarcinoma after complete resection. Methods: A total of 11,020 patients with lung surgery were screened to determine completely resected EGFR-mutant stage III-pN2 lung adenocarcinoma. Patients were excluded if they received preoperative therapy or postoperative radiation therapy (PORT). The time from surgery to LRR was recorded. Clinicopathological variables with statistical significance predicting LRR in the multivariate Cox regression were incorporated into the competing risk nomogram. Patients were then sub-grouped based on different recurrence risk as a result of the nomogram. Results: Two hundred and eighty-eight patients were enrolled, including 191 (66.3%) with unforeseen N2 (IIIA1-2), 75 (26.0%) with minimal/single station N2 (IIIA3), and 22 (7.6%) with bulky and/or multilevel N2 (IIIA4). The 2-year overall cumulative incidence of LRR was 27.2% (confidence interval [CI], 16.3%-38.0%). IIIA4 disease (hazard ratio, 2.65; CI, 1.15-6.07; P=0.022) and extranodal extension (hazard ratio, 3.33; CI, 1.76-6.30; P<0.001) were independent risk factors for LRR and were incorporated into the nomogram. Based on the nomogram, patients who did not have any risk factor (low-risk) had a significantly lower predicted 2-year incidence of LRR than those with any of the risk factors (high-risk; 4.6% vs 21.9%, P<0.001). Conclusions: Pre-treatment bulky/multilevel N2 and pathological extranodal extension are risk factors for locoregional recurrence in EGFR-mutant stage III-pN2 lung adenocarcinoma. Intensive adjuvant therapies and active follow-up should be considered in patients with any of the risk factors.

16.
J Korean Med Sci ; 35(31): e260, 2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32776721

RESUMO

BACKGROUND: To elucidate the achievement rates of imaging remission and to examine the characteristics associated with imaging remission status among patients with rheumatoid arthritis (RA) who have attained clinical remission. METHODS: Ninety-seven patients with RA patients who had attained clinical remission, defined by DAS28-ESR < 2.6 were enrolled. Power Doppler ultrasonography (PDUS) was performed on 16 joints and 2 tendons, including the first to third metacarpophalangeal, second and third proximal interphalangeal, radiocarpal (RC), second and third metatarsophalangeal joints, and extensor carpi ulnaris tendons. They were graded based on a dichotomous assessment. The clinical and laboratory data of patients who had attained imaging remission were compared to those of patients who had attained only clinical remission. RESULTS: The imaging remission rate was 51.5% in patients who had attained clinical remission. Forty-seven patients (48.5%) were PDUS positive. Power Doppler was detected most frequently in the right RC joint (n = 40). PDUS positive patients had higher evaluator global assessment (EGA) scores (P < 0.001) than PDUS negative patients. PDUS positive patients also had higher clinical disease activity index and simplified clinical disease activity index scores than PDUS negative patients. Patients who had attained imaging remission had lower pain scores and used nonsteroidal anti-inflammatory drugs less frequently. Patients who had attained imaging remission had higher rheumatoid factor (RF) and anti-cyclic citrullinated peptide levels. A low EGA score was found to be a predictor of imaging remission achievement among patients who had attained clinical remission. CONCLUSION: Only 51.5% of the patients with RA who had attained clinical remission were also in imaging remission. Patients who had attained imaging remission had lower EGA scores and higher RF levels than patients who had attained only clinical remission.


Assuntos
Artrite Reumatoide/diagnóstico , Articulações/diagnóstico por imagem , Tendões/diagnóstico por imagem , Ultrassonografia Doppler , Adulto , Idoso , Artrite Reumatoide/diagnóstico por imagem , Artrite Reumatoide/patologia , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Peptídeos Cíclicos/análise , Valor Preditivo dos Testes , Fator Reumatoide/análise , Índice de Gravidade de Doença
17.
Carbohydr Res ; 496: 108114, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32823055

RESUMO

The interaction between sugars and water molecules plays a crucial role in exploring the complex metabolic reactions of living systems. The fully random conformational search is employed to investigate the first hydration shell structure of glucose. The circumference of glucose is divided into three hydrate sites according to the location of water molecules. Especially, four water molecules can saturate hydrate site AI and while more water molecules will be required to saturate hydrate sites AIII and AII. Moreover, the hydration energies of water molecules at different hydration region indicate that the competition of the three hydrate sites for water molecules changes dynamically with the increase of water molecules. In addition, we calculate the Raman spectra of the hydrated glucose, which are in good agreement with the experimental and theoretical Raman spectra of the glucose solution.


Assuntos
Glucose/química , Teoria Quântica , Água/química , Configuração de Carboidratos , Simulação de Dinâmica Molecular , Análise Espectral Raman
18.
DNA Cell Biol ; 39(6): 1041-1050, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32401536

RESUMO

Ovarian cancer (OC) is one of gynecological malignancies that seriously affects women's health. Mounting evidence demonstrated that long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) play important roles in various biological processes related to the pathogenesis of OC. This research aimed to investigate the regulatory mechanism of lncRNA SCAMP1/miR-137/CXCL12 (C-X-C motif chemokine ligand 12) axis on OC progression. In this study, we found that SCAMP1 was highly expressed in OC cells, which promoted OC cell invasion and angiogenesis. In addition, our research confirmed that SCAMP1 could bind with miR-137, and SCAMP1 sponged miR-137 to accelerate the progression of OC. We also observed that CXCL12 was a downstream target gene for miR-137, and miR-137 targeted CXCL12 to participate in the regulation of OC. Finally, through TCGA database, we found that SCAMP1 (or CXCL12) was upregulated as well as miR-137 was downregulated in OC tissues, and high (or low) level of them was associated with poor prognosis. miR-137 expression was negatively correlated with SCAMP1 (or CXCL12) expression, and SCAMP1 expression was positively correlated with CXCL12 expression in OC. In summary, our study clarified the role of SCAMP1/miR-137/CXCL12 axis in OC, and this finding may provide a potential therapeutic target of OC.


Assuntos
Quimiocina CXCL12/genética , MicroRNAs/genética , Neovascularização Patológica/genética , Neoplasias Ovarianas/patologia , RNA Longo não Codificante/genética , Proteínas de Transporte Vesicular/genética , Sequência de Bases , Linhagem Celular Tumoral , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Invasividade Neoplásica/genética , Neoplasias Ovarianas/irrigação sanguínea , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/genética , Prognóstico
19.
Materials (Basel) ; 13(6)2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32192188

RESUMO

Structures made of Roller Compacted Concrete (RCC) may be subjected to dynamic loads during their service life. Understanding the dynamic material properties of RCC and the performance of RCC structures is essential for better analysis and design of RCC structures. As full-scale tests are often unaffordable, numerical simulation methods are continuously employed. However, in numerical simulations, determining a reasonable constitutive relationship for RCC materials is still limited due to the complexity of the composite and the special rolling and compacting construction technology. In this paper, the triaxial compressive test and split Hopkinson pressure bar (SHPB) experimental results for RCC are introduced as an experimental foundation. Parameter calibrations and modifications in terms of the strength yield surface, the strain rate effect and the failure criterion for the RCC materials are presented. Numerical verification is illustrated for simulating the SHPB experiment and predicting the dynamic compressive characteristics of RCC specimens with a modified HJC model. The results reveal that the simulation results for the modified model have better agreement with the test data than those with the model before modification and have better simulation results. Sensitivity studies of the key parameters on the yield surface of the modified HJC model are conducted to improve the simulation effect for numerically predicting the performance of RCC structures exposed to explosive and impact loads.

20.
IEEE Trans Vis Comput Graph ; 26(6): 2204-2218, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-30530330

RESUMO

An importance measure of 3D objects inspired by human perception has a range of applications since people want computers to behave like humans in many tasks. This paper revisits a well-defined measure, distinction of 3D surface mesh, which indicates how important a region of a mesh is with respect to classification. We develop a method to compute it based on a classification network and a Markov Random Field (MRF). The classification network learns view-based distinction by handling multiple views of a 3D object. Using a classification network has an advantage of avoiding the training data problem which has become a major obstacle of applying deep learning to 3D object understanding tasks. The MRF estimates the parameters of a linear model for combining the view-based distinction maps. The experiments using several publicly accessible datasets show that the distinctive regions detected by our method are not just significantly different from those detected by methods based on handcrafted features, but more consistent with human perception. We also compare it with other perceptual measures and quantitatively evaluate its performance in the context of two applications. Furthermore, due to the view-based nature of our method, we are able to easily extend mesh distinction to 3D scenes containing multiple objects.

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