Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 121
Filtrar
1.
Anim Sci J ; 92(1): e13513, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33586241

RESUMO

Phytase is an enzyme that has the ability to release phosphorous (P) from phytate by hydrolyzing inositol-phosphate linkages. Recently, thermostable phytases have gained great consideration because the reduction in phytase activity was found when exposed to heat during feed pelleting. In this study, the effects of the granular thermostable phytase (Aspergillus niger) on growth performance and nutrient utilization of broilers were investigated. A total of 96 21-day-old Arbor Acres broilers were randomly distributed into six treatments including basal diet (control) and basal diet supplemented with 500, 1,000, 2,000, 4,000, 8,000 U of phytase/kg. In general, the metabolizable energy (ME) and the apparent and true availability of dry matter (DM), organic matter (OM), ether extract (EE), crude protein (CP), and amino acids (AA) showed both linearly (p < .01) and quadratically (p < .01) increase with increasing levels of phytase in the diet. Additionally, diet supplementation with phytase could improve (p < .05) body weight (BW), average daily gain (ADG), and feed/gain (F/G) on day 42 compared with the control. The results suggested that diet supplementation of the granular thermostable phytase in the crumbled pellets could improve chicken growth performance and nutrient utilization.

2.
Medicine (Baltimore) ; 100(7): e24527, 2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33607783

RESUMO

ABSTRACT: A history of transient ischemic attack, severity of disease, urinary output, hematocrit, hypocapnia, and hypotension during direct revascularization (superficial temporal artery to middle cerebral artery [STA-MCA]) in patients with Moyamoya disease (MMD) may lead to a poor prognosis, however, to our knowledge evidence for end-tidal carbon dioxide (ETCO2) targets is lacking. Within the ranges of standardized treatment, the article was primarily designed to study the risk factors associated with the neurologic outcomes during STA-MCA for MMD especially including ETCO2 ranges and the duration in different groups. The primary goals of this study were to investigate the risk factors for neurologic deterioration and explore the association between ETCO2 ranges and neurologic outcome during general anesthesia for STA-MCA.This retrospective observational study included 56 consecutively adult Moyamoya patients who underwent STA-MCA under general anesthesia between January 2015 and August 2019. ETCO2 was summarized per patient every 5 minutes. Clinical outcome was assessed with clinical presentation, computed tomography findings, magnetic resonance imaging findings, cerebral angiography, and the modified Rankin Scale scores at discharge as main outcome measure. The outcomes were also compared for the duration of surgery, anesthesia, and the length of stay.A total of 56 patients were studied, all patients had comprehensive ETCO2 measurements. The incidence of postoperative complications was 44.6% (25/56). There was no association between age, sex, hypertension, diabetes, smoking history, drinking history, sevoflurane use, invasive arterial blood pressure monitoring, combined encephalomyosynangiosis and postoperative complications. Duration of surgery (P = .04), anesthesia (P = .036), hospital stay (P = .023) were significant correlates of postoperative complications. In the multiple logistic regression model, they were not the significant predictors. The ETCO2 ranges and the length of time in different groups within the current clinical setting was not associated with postoperative complications (P > .05).Within a standardized intraoperative treatment strategy, we found that postoperative complications had no significant correlation with sex, age, hypertension, diabetes, smoking history, drinking history, invasive arterial blood pressure monitoring, combined encephalomyosynangiosis, or sevoflurane use. Further, hypocapnia and hypercapnia during STA-MCA were not found to be associated with postoperative complications in patients with MMD.

3.
Environ Pollut ; 275: 116587, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33582626

RESUMO

Traditional composting has already shown a certain effect in eliminating antibiotic residues, antibiotic-resistant bacteria (ARBs), and antibiotic resistance genes (ARGs). It is worth noting that the rebounding of ARGs and the succession of the bacterial community during conventional aerobic composting are still serious threats. Considering the probable risk, improved and adaptable technologies are urgently needed to control antibiotic resistance efficiently. This study monitored how thermophilic aerobic composting affected the ARGs, as well as the bacterial diversity during the composting of cow manure spiked with sulfamethoxazole (SMX) at different concentrations. Results showed that the degradation of SMX was enhanced during thermophilic aerobic composting (control > SMX25 > SMX50 > SMX100) and was no longer detected after 20 days of composting. High temperature or heat significantly stimulated the rebounding of certain genes. After 35 days, the abundance of detected genes (sul2, sulA, dfrA7, and dfrA1) significantly decreased (p < 0.05) in control and antibiotic-spiked treatments, except for sul1. The addition of three concentrations of SMX elicited a sharp effect on bacterial diversity, and microbial structure in SMX25 led to significant differences with others (p < 0.05). The network analysis revealed more rigorous interactions among ARGs and abundant genera, suggesting that the host of ARGs potentially increased at low concentrations of SMX. Especially, genera g_norank_f__Beggiatoaceae, Ruminiclostridium, Caldicoprobacter, g_norank_o_MBA03, Hydrogenispora, and Ruminiclostridium_1 were major potential hosts for sul1. In conclusion, the rebounding of ARGs could be intermitted partially, and more efficient control of antibiotic resistance could be achieved in the thermophilic composting compared to conventional methods.

4.
BMC Cardiovasc Disord ; 21(1): 24, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413149

RESUMO

BACKGROUND: The present study aimed to assess the correlation of fibroblast growth factor (FGF)-23 expression with clinical characteristics, then further explore its value in predicting 2-year in-stent restenosis (ISR) risk in coronary heart disease (CHD) patients underwent percutaneous coronary intervention (PCI) with drug-eluting stent (DES). METHODS: In this prospective, single-center, observational study, totally 214 CHD patients treated by PCI with DES were consecutively recruited, and peripheral blood samples were collected prior to PCI with DES for serum samples isolation. Following, FGF-23 level in the serum samples was detected via enzyme linked-immuno-sorbent Assay. The follow-up coronary angiography was performed at 1 year and 2 years after PCI or if suspected ISR symptoms occurred. RESULTS: FGF-23 was positively correlated with fasting blood-glucose, insulin resistance, serum creatinine, serum uric acid, LDL-C, high-sensitivity C-reactive protein, cardiac troponin I and N-terminal-proB-type natriuretic peptide, while was negatively associated with HDL-C and left ventricular ejection fraction (all P < 0.01). Furthermore, FGF-23 was positively correlated with hypercholesteremia, hyperuricemia and family history of CAD (all P < 0.05). However, it did not correlate with other chronic complications, biochemical indexes, lesion features or PCI parameters (all P > 0.05). Moreover, FGF-23 level was higher in 2-year ISR patients (n = 38) compared to 2-year non-ISR patients (n = 176) (P < 0.001), and receiver operating characteristic curve indicated that FGF-23 was of good value in predicting 2-year ISR risk (AUC 0.828, 95% CI 0.761-0.896). CONCLUSION: FGF-23 correlates with endocrine and metabolism dysregulation, worse cardiac and renal function, inflammation level, stenosis degree of target lesion, and serves as an independent risk factor for 2-year ISR risk in CHD patients underwent PCI with DES.

5.
J Psychiatr Res ; 135: 37-46, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33445059

RESUMO

Only a few studies investigated the impact of quarantine on anxiety of general population during a second wave of COVID-19 breakout. We aimed to compare anxiety levels of quarantined and non-quarantined people and investigate factors affecting anxiety during the second COVID-19 pandemic. A total of 1837 participants were included in this cross-sectional study. Anxiety was measured by the State-Trait Anxiety Inventory (STAI). Participants were divided into the quarantined group (QG) and non-quarantined group (Non-QG). The mean STAI-S score in the QG was significantly higher than Non-QG (41.8 ± 11.2 vs 40.01 ± 9.9), so was the proportion of severe state anxiety (11.6% vs 5.5%). Males in the QG were significantly more anxious than females evaluated by both STAI-S and STAI-T. High income was independent protective factors while moderate or bad health status and high trait anxiety level were independent risk factors for severe state anxiety. In conclusion, the COVID-19 confinement could significantly increase anxiety of quarantined people. Males were more vulnerable to the quarantine of COVID-19 with significantly increased anxiety level than females. The results suggest that attention should be paid to anxiety during a second round of quarantine due to COVID-19 and are of help in planning psychological interventions.

6.
Plant Physiol Biochem ; 159: 301-311, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33418189

RESUMO

AIMS: Alkaline salt is one of the most devastating environmental factors limiting alfalfa productivity, however, the mechanisms underlying adaptation of alfalfa to alkaline remain unclear. Our aim is to investigate proteomic and metabolomic differences in growth and root of alfalfa under alkaline salt in Rhizobium-alfalfa symbiotic relationships. METHODS: Rhizobium-inoculated and non-inoculated alfalfa plants were treated with 200 mmol/L NaHCO3 to investigate physiological, metabolic, and proteomic responses of root-nodule symbiosis under alkaline-induced stress, using an integrated approach combining metabolome and proteome analysis with measurements of physiological parameters. RESULTS: The improved tolerance to alkalinity was observed in RI-plants compared with NI-plants. RI-plants accumulated more proline and MDH, and had higher antioxidant activity and relatively high RWC but low MDA content and low Na+/K+ ratio. The stress-related genes (P5CS, GST13, H+-Ppase, NADP-Me, SDH, and CS) were actively upregulated in RI plants under alkaline stress. In RI-plants, damage caused by alkaline stress was mainly alleviated by decreasing oxidative damage, enhancing the organic acid and amino acid metabolic processes, and scavenging harmful ROS by activating the phenylpropanoid biosynthetic pathway. CONCLUSIONS: We revealed distinct proteins and metabolites related to alkali tolerance in RI-plants compared to NI-plants. Alkali tolerance of rhizobia-inoculated alfalfa was enhanced by altered proteins and metabolic processes as well as decreased oxidative damage.


Assuntos
Medicago sativa , Estresse Oxidativo , Rhizobium , Álcalis/toxicidade , Medicago sativa/efeitos dos fármacos , Medicago sativa/microbiologia , Estresse Oxidativo/fisiologia , Proteômica , Rhizobium/fisiologia
7.
Artigo em Inglês | MEDLINE | ID: mdl-33360679

RESUMO

ASK120067, an oral irreversible tyrosine kinase inhibitor (TKI) targeting the epidermal growth factor receptor (EGFR), is formulated for the management of patients with non-small cell lung cancer (NSCLC) who harbor T790M resistant and EGFR active mutations. Two rapid and high-throughput methods based on liquid chromatography-tandem mass spectrometry to detect ASK120067 and its primary metabolite CCB4580030 in human plasma were developed and applied in the clinical trials. A protein precipitation method using acetonitrile coupled with a gradient elution separation in a BEH C18 column (1.7 µm, 2.1 × 50 mm) was used to process plasma and separation analytes. The chromatographic separation was performed on the mobile phase of 5 mM ammonium acetate in water with 0.1% formic acid (A) and acetonitrile (B), and the flow rate was 0.4 mL/min. The multiple reaction monitoring (MRM) mode was selected to monitor the precursor-to-product ion transitions of m/z 546.2 â†’ m/z 431.2 for ASK120067 and m/z 532.1 â†’ m/z 420.2 for CCB4580030 at the positive ionization mode. The precision and accuracy of the two methods for ASK1200067 and CCB4580030 were within acceptable range for the linear range in 5.00-5000 ng/mL and 0.500-500 ng/mL, respectively. Further stabilities for the two analytes and internal standard were also investigated covered the entire experimental process beginning from harvesting whole blood to plasma extraction and analysis. ASK120067 was then administered without issue onto a dose-escalation, the first-in-human Phase I clinical trial in Chinese NSCLC patients to determine the pharmacokinetics of oral ASK120067 administration.

8.
Ann Palliat Med ; 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33302639

RESUMO

BACKGROUND: Pulse-taking is widely used for diagnosis and treatment in traditional Chinese medicine (TCM), and protein complexes in serum perform various biological functions. The Balanced constitution is one of the major constitutions in TCM, people with Balanced constitution can also share some common characteristics with unbalanced constitution types. METHODS: Blue native polyacrylamide gel electrophoresis (BN-PAGE) was applied to the serum of 25 people with balanced constitutions. The patterns of the protein complexes could be recognized according to the number, molecular weight, and intensity of the gel bands. All of the individual bands from these patterns were cut and in-gel-digested with trypsin, followed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis for protein identification and biological function analysis. RESULTS: The protein complex patterns were roughly categorized as type A and B with high stability and reproducibility, and there were 15 and 16 gel bands in type A and type B, respectively. Among the 25 serum samples, 14 belonged to type A, and 11 belonged to type B. High-abundance proteins significantly decreased from 99% to 44% after BN-PAGE separation. The unique proteins in type A were mainly related to lipid metabolism, while the unique proteins in type B were involved in biological processes related to immune response and inflammatory regulation. The Qi-deficiency constitution converted score of type A was higher than that of type B, while the Damp-heat constitution converted score of type A was lower than that of type B. CONCLUSIONS: Our study provided an objective reference for diagnosis and prognosis, which might lay a foundation for establishing the characteristic protein complex spectra of all of the TCM constitutions.

9.
J Exp Clin Cancer Res ; 39(1): 286, 2020 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-33317572

RESUMO

BACKGROUND: Increasing evidence has revealed the close link between mitochondrial dynamic dysfunction and cancer. MIEF2 (mitochondrial elongation factor 2) is mitochondrial outer membrane protein that functions in the regulation of mitochondrial fission. However, the expression, clinical significance and biological functions of MIEF2 are still largely unclear in human cancers, especially in ovarian cancer (OC). METHODS: The expression and clinical significance of MIEF2 were determined by qRT-PCR, western blot and immunohistochemistry analyses in tissues and cell lines of OC. The biological functions of MIEF2 in OC were determined by in vitro and in vivo cell growth and metastasis assays. Furthermore, the effect of MIEF2 on metabolic reprogramming of OC was determined by metabolomics and glucose metabolism analyses. RESULTS: MIEF2 expression was significantly increased in OC mainly due to the down-regulation of miR-424-5p, which predicts poor survival for patients with OC. Knockdown of MIEF2 significantly suppressed OC cell growth and metastasis both in vitro and in vivo by inhibiting G1-S cell transition, epithelial-to-mesenchymal transition (EMT) and inducing cell apoptosis, while forced expression of MIEF2 had the opposite effects. Mechanistically, mitochondrial fragmentation-suppressed cristae formation and thus glucose metabolism switch from oxidative phosphorylation to glycolysis was found to be involved in the promotion of growth and metastasis by MIEF2 in OC cells. CONCLUSIONS: MIEF2 plays a critical role in the progression of OC and may serve as a valuable prognostic biomarker and therapeutic target in the treatment of this malignancy.

10.
Bioresour Technol ; 320(Pt B): 124403, 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-33217693

RESUMO

Current work for animal manure processing is not up to the required standards and hence are not supposed to reflect the actual performance in antibiotic resistance control. As a result, this study carried out temperature-controlled aerobic composting, with sulfamethoxazole (SMX) as a typical antibiotic. The results of four different treatments demonstrated that temperature, water content, C/N ratio, EC, and pH showed no significant (p > 0.05) difference. Antibiotic resistance genes (ARGs) significantly decreased in the initial 10 days of the thermophilic phase, but the abundance of sul1 and sul2 increased greatly after 30 days. Moreover, ARGs were closely related with each other during the late stages of composting. A noteworthy effect of composting properties, especially temperature on bacterial community, which then had a positive effect on ARGs abundances. These findings provided evidence that the standard composting was still insufficient to control antibiotic resistance.

11.
Micromachines (Basel) ; 11(11)2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-33142822

RESUMO

Insertion loss and crosstalk noise will influence network performance severely, especially in optical networks-on-chip (ONoCs) when wavelength division multiplexing (WDM) technology is employed. In this paper, an insertion loss and crosstalk analysis model for WDM-based torus ONoCs is proposed to evaluate the network performance. To demonstrate the feasibility of the proposed methods, numerical simulations of the WDM-based torus ONoCs with optimized crossbar and crux optical routers are presented, and the worst-case link and network scalability are also revealed. The numerical simulation results demonstrate that the scale of the WDM-based torus ONoCs with the crux optical router can reach 6 × 5 or 5 × 6 before the noise power exceeds the signal power, and the network scale is 5 × 4 in the worst case when the optimized crossbar router is employed. Additionally, the simulated results of OptiSystem reveal that WDM-based torus ONoCs have better signal transmission quality when using the crux optical router, which is consistent with previous numerical simulations. Furthermore, compared with the single-wavelength network, WDM-based ONoCs have a great performance improvement in end-to-end (ETE) delay and throughput according to the simulated results of OPNET. The proposed network analysis method provides a reliable theoretical basis and technical support for the design and performance optimization of ONoCs.

12.
Micromachines (Basel) ; 11(11)2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33182518

RESUMO

Optical networks-on-chips (ONoCs) is an effective and extensible on-chip communication technology, which has the characteristics of high bandwidth, low consumption, and low delay. In the design process of ONoCs, power loss is an important factor for limiting the scalability of ONoCs. Additionally, the optical signal-to-noise ratio (OSNR) is an index to measure the quality of ONoCs. Nowadays, the routing algorithm commonly used in ONoCs is the dimension-order routing algorithm, but the routing paths selected by the algorithm have high power loss and crosstalk noise. In this paper, we propose a 5×5 all-pass optical router model for two-dimensional (2-D) mesh-based ONoCs. Based on the general optical router model and the calculation models of power loss and crosstalk noise, a novel algorithm is proposed in ordder to select the routing paths with the minimum power loss. At the same time, it can ensure that the routing paths have the approximately optimal OSNR. Finally, we employ the Cygnus optical router to verify the proposed routing algorithm. The results show that the algorithm can effectively reduce the power loss and improve the OSNR in the case of network sizes of 5×5 and 6×6. With the increase of the optical network scale, the algorithm can perform better in reducing the power loss and raising the OSNR.

13.
Cancer Manag Res ; 12: 8893-8902, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33061585

RESUMO

Objective: To investigate the anti-tumor effects of programmed cell death protein 1 (PD-1) scFv-secreting EGFR-chimeric antigen receptor-modified (CAR)-T cells against gastric cancer. Methods: Second-generation EGFR-CAR-T cells and fourth-generation PD-1 scFv-secreting EGFR-CAR-T cells were engineered. The anti-tumor activities of chimeric antigen receptor-modified (CAR)-T cells were analyzed in vitro by long-term co-culture with gastric cancer cells. The tumor scavenging capacity in vivo was evaluated in xenograft and PDX mouse models. Results: EGFR-CAR-T cells secreting PD-1 scFv showed enhanced long-term tumor cell killing capacity in vitro. These cells also showed significant anti-tumor effect in the subcutaneous xenograft model of gastric cancer as well as in the PDX model, and autocrine PD-1 antibody secretion significantly increased tumor infiltration of the CAR-T cells. Conclusion: EGFR-CAR-T cells secreting PD-1 scFv are highly effective against gastric cancer and offer new insights into anti-cancer immunotherapy.

14.
Artigo em Inglês | MEDLINE | ID: mdl-32898953

RESUMO

Objective: ZEA has estrogen-like effects. Our previous study has shown that ZEA (0.5-1.5 mg/kg) could induce abnormal uterine proliferation through TGF signaling pathway. In order to further study the other regulatory networks of uterine hypertrophy caused by ZEA, the potential mechanism of ZEA on porcine endometrial epithelial cells was explored by the Illumina Hiseq 2000 sequencing system in this study. Methods: The PECs were treated with ZEA at 0 (ZEA0), 5 (ZEA5), 20 (ZEA20), and 80 (ZEA80) µmol/L for 24 h. The collected cells were subjected to cell cycle, RNA-seq, qRT-PCR, immunofluorescence, and western blot analysis. Results: The proportion of cells in the S and G2 phases decreased (p < 0.05), but the proportion of cells in the G1 phase increased (p < 0.05) in the ZEA80 treatment. Data analysis revealed that the expression of Wnt pathway-related genes, estrogen-related genes, and MAPK pathway-related genes increased (p < 0.05), but the expression of genetic stability genes decreased (p < 0.05) with increasing ZEA concentrations. The relative mRNA and protein expression of WNT1, ß-catenin, glycogen synthase kinase 3ß (GSK-3ß) were increased (p < 0.05) with ZEA increasing, while the relative mRNA and protein expression of cyclin D1 (CCND1) was decreased (p < 0.05). Moreover, our immunofluorescence results indicate that ß-catenin accumulated around the nucleus from the cell membrane and cytoplasm with increasing ZEA concentrations. Conclusion: In summary, ZEA can activate the Wnt/ß-catenin signaling pathway by up-regulating WNT1 and ß-catenin expression, so as to promote the proliferation and development of porcine endometrial epithelial cells. At the same time, the up-regulation of GSK-3ß and down-regulation of CCND1, as well as the mRNA expression of other pathway related genes indicated that other potential effects of ZEA on the uterine development need further study.

15.
Int J Pept Res Ther ; : 1-9, 2020 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-32901205

RESUMO

Phage display is an effective and powerful technique that provides a route to discovery unique peptides targeting to tumor cells. Specifically binding peptides are considered as the valuable target directing molecule fragments with potential efficiency to improve the current tumor clinic, and offer new approaches for tumor prevention, diagnosis and treatment. We focus on the recent advances in the isolation of tumor-targeting peptides by biopanning methods, with particular emphasis on molecular imaging, and pharmaceutical targeting therapy.

16.
Tissue Cell ; 67: 101440, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32971457

RESUMO

Long non-coding RNA predicting cardiac remodeling (lnc LIPCAR) was implicated in several human diseases, while its role in atrial fibrillation (AF) remained poorly understood. Our study aimed to discover the role of LICPAR played in AF. Samples of atrial muscle tissues from patients diagnosed with sinus rhythm (SR) and atrial fibrillation (AF) were collected, and human atrial fibroblasts were isolated and identified under immunofluorescence staining. After Angiotensin II (Ang II, as a activator of TGF-ß) stimulation with LICPAR overexpression or knockdown, the viability and proliferation of atrial fibroblasts were respectively determined using cell counting kit-8 (CCK-8) assay and clone formation assay. Relative expressions of LICPAR, fibrosis- and transforming growth factor-ß (TGF-ß)/Smad2/3-pathway related proteins were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed. LICPAR and TGF-ß1 were upregulated and were positively correlated in atrial muscle tissues from AF. Atrial fibroblasts were identified as Vimentin positive. Further analysis indicated that Ang II enhanced the levels of LIPCAR, Smad2/3 phosphorylation and α-smooth muscle actin (α-SMA). Also, upregulating LIPCAR further promoted the promotive effects of Ang II on levels of LIPCAR, Collagen I, Collagen II, α-SMA and Smad2/3 phosphorylation, cell viability and proliferation of atrial fibroblasts, whereas silencing LIPCAR resulted in opposite effects. LICPAR regulates atrial fibrosis via modulating TGF-ß/Smad pathway, which provided a potential therapeutic method for AF in clinical practice in the future.

17.
Environ Res ; 191: 110228, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32950517

RESUMO

Membrane fouling has become the one of main obstacles for the widespread application of membrane technology in water treatment processes. Coagulation as pretreatment is proven to be effective for the alleviation of membrane fouling. In this study, the influence of humic acid (HA)/sodium alginate (SA) fractions in the structure and resistance of cake layer on the membrane surface was investigated. The presence of SA at an appropriate fraction could facilitate the formation of large and loosely branched flocs and thereby form a more permeable cake layer on the membrane surface due to good bridging and charge neutralization abilities of SA molecules. The particle image velocimetry (PIV) technique was employed for monitoring the dynamic formation process of cake layer under different HA/SA fractions. The cake layer with a higher thickness was observed to be rapidly formed on the membrane surface at the presence of SA in water. According to the theoretical analysis, the membrane fouling in coagulation-ultrafiltration (UF) combined system demonstrated to be highly dependent on the size and intra-porosity of flocs. The fractal dimension of flocs might have an impact on the resistance of cake layer through affecting the porosity of aggregated flocs. The SA molecules could be used as the coagulant aid for effective alleviation of membrane fouling and the improvement of filtration performance in a coagulation-UF combined system.

18.
Plant Physiol Biochem ; 154: 538-546, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32912487

RESUMO

The sulfite reductase gene in Medicago sativa L. (MsSiR) encodes sulfite reductase (SiR) and catalyses the conversion of sulfite to sulfate in the sulfite assimilation pathway. In this study, we investigated the role of MsSiR in alfalfa by generating transgenic alfalfa that ectopically expressed MsSiR under the control of the CaMV35S promoter. The differences in alkali tolerance between the MsSiR-overexpressing and wild-type (WT) plants were analyzed, and the MsSiR-overexpressing plants exhibited an improved phenotype under alkali stress. Compared to WT plants, these plants demonstrated improved antioxidant activity as well as decreased H2O2 and O2- contents and increased glutathione reduced (GSH), Cysteine (Cys) and glutathione oxidized (GSSG) contents. MsSiR-overexpressing plants also exhibited high levels of adenosyl phosphosulfate reductases (APR), sulfite oxidase (SO) and MsSiR expression under alkali stress. It was speculated that MsSiR is involved in sulfur metabolism pathways, including the stabilization of sulfate and sulfite levels and the synthesis of GSH. These two processes achieve alkali tolerance by positively regulating the detoxification and antioxidant activities of alfalfa.


Assuntos
Álcalis/efeitos adversos , Glutationa/análise , Medicago sativa , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/genética , Proteínas de Plantas/genética , Antioxidantes/análise , Peróxido de Hidrogênio , Medicago sativa/enzimologia , Medicago sativa/genética , Plantas Geneticamente Modificadas/enzimologia , Estresse Fisiológico
19.
Brain Behav ; 10(9): e01715, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32812388

RESUMO

BACKGROUND: Postpartum depression (PPD) is a common mental disease happens in perinatal period. Ketamine as an anesthesia and analgesia drug has been used for a long time. In recent years, ketamine is proved to have an antidepression effect with a single administration. We hypothesized that intraoperative ketamine can reduce postpartum depressive symptoms after cesarean delivery. METHODS: In a randomized, double-blind, placebo-controlled study trail, healthy women scheduled for cesarean delivery were randomly assigned to receive intravenous ketamine (0.25 mg/kg diluted to 5 ml with 0.9% saline) or placebo (5 ml of 0.9% saline) within 5 min following clamping of the neonatal umbilical cord. The primary outcome was the degree of postpartum depressive symptoms, which was evaluated by Edinburgh Postnatal Depression Scale (EPDS, a threshold of 9/10 was used) at 1 week, 2 weeks, and 1 month after delivery. The secondary outcome was the numerical rating scale (NRS) score of pain at 2 days postpartum. This trail is registered in the Chinese Clinical Trial Registry, number ChiCTR1900022464. RESULTS: Between 26 January 2019 and 15 July 2019, 502 subjects were screened and 330 were randomly allocated: 165 (50%) to the ketamine group and 165 (50%) to the placebo group. There were significant differences in the degree of postpartum depressive symptoms between subjects in the ketamine group and the placebo group at 1 week postpartum (13.1% vs. 22.6%, respectively; p = .029). However, no difference was found between subjects in the two groups at 2 weeks (11.8% vs. 16.8%, respectively; p = .209) and 1 month postpartum (10.5% vs. 14.2%, respectively; p = .319). The NRS score of wound pain (3.0 ± 0.9 vs. 4.0 ± 1.0, respectively; p < .001) and uterine contraction pain (3.0 ± 0.9 vs. 4.1 ± 0.9, respectively; p < .001) was lower in the ketamine group at 2 days postpartum compared with placebo group. The prevalence of headache, hallucination, and dizziness was higher in the ketamine group than the placebo group during the operation. CONCLUSIONS: Operative intravenous ketamine (0.25 mg/kg) can reduce the postpartum depressive symptoms for 1 week. The long-time effect is remained to be seen.

20.
Integr Cancer Ther ; 19: 1534735420942587, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32787468

RESUMO

PURPOSE: To explore a new therapeutic option for patients with hepatocellular carcinoma (HCC), the efficacy and safety of a group of traditional Chinese medicines (Banxia XieXin recipe) as monotherapy for patients with advanced HCC was studied. MATERIALS AND METHODS: The study included 68 patients with advanced HCC from August 16,2016 to August 15,2019 for analysis. These eligible patients received treatment with Banxia XieXin recipe for at least 1 month. The primary endpoints were progression-free survival (PFS) and overall survival (OS). The secondary efficacy endpoints included objective response rate (ORR) and disease control rate (DCR). In addition, safety was also assessed. RESULTS: The median treatment duration of these 68 patients was 10.3 months (range = 1.6-33.5 months), and follow-up is still ongoing. The median PFS was 6.07 months (95% confidence interval [CI] = 3.748-8.392 months), and the median OS was 12.60 months (95% CI = 8.019-17.181 months). The ORR was 10.3% and the DCR was 41.2%. In the subgroup analysis, the median OS in the transcatheter arterial chemoembolization (TACE) group was not reached, and the median OS in the NO TACE group was 11.30 months (95% CI = 3.219-19.381 months). In addition, no drug-related serious adverse events were observed during the study. CONCLUSION: This is the first clinical analysis of traditional Chinese medicine as a single treatment for advanced HCC. The obtained results are encouraging as they suggest that this panel of Chinese herbs is safe and it may be effective for patients with advanced HCC in a real-world clinical setting.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA