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1.
Int J Mol Sci ; 22(15)2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34360535

RESUMO

The aims of this study were to develop the magnolol-chitosan films and study the positive effect of the combination of magnolol and chitosan. The addition of magnolol made the magnolol-chitosan films exhibit higher density (1.06-1.87 g/cm3), but the relatively lower water vapor permeability (12.06-7.36 × 10-11·g·m-1·s-1·Pa-1) and water content (16.10-10.64%). The dense and smooth surface and cross-section of magnolol-chitosan films were observed by environmental scanning electron microscopy (ESEM) images. The interaction of magnolol and chitosan was observed by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). After the addition of magnolol, the antioxidant capacity of magnolol-chitosan films was increased from 18.99 to 82.00%, the growth of P. aeruginosa was inhibited and the inhibition percentage of biofilm formation was increased from 30.89 to 86.04%. We further verified that the application of magnolol-chitosan films on chilled pork significantly reduced the increases in pH value, inhibited the growth of microorganisms and extended the shelf life. Results suggest that magnolol had a positive effect on magnolol-chitosan films and could be effectively applied to pork preservation.


Assuntos
Anti-Infecciosos/farmacologia , Antioxidantes/farmacologia , Compostos de Bifenilo/farmacologia , Quitosana/química , Conservação de Alimentos/métodos , Lignanas/farmacologia , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa/efeitos dos fármacos , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Embalagem de Alimentos/métodos , Carne de Porco/análise , Infecções por Pseudomonas/microbiologia , Suínos
2.
Philos Trans R Soc Lond B Biol Sci ; 376(1832): 20200095, 2021 08 30.
Artigo em Inglês | MEDLINE | ID: mdl-34247503

RESUMO

The tempo of sex chromosome evolution-how quickly, in what order, why and how their particular characteristics emerge during evolution-remains poorly understood. To understand this further, we studied three closely related species of African clawed frog (genus Xenopus), that each has independently evolved sex chromosomes. We identified population polymorphism in the extent of sex chromosome differentiation in wild-caught Xenopus borealis that corresponds to a large, previously identified region of recombination suppression. This large sex-linked region of X. borealis has an extreme concentration of genes that encode transcripts with sex-biased expression, and we recovered similar findings in the smaller sex-linked regions of Xenopus laevis and Xenopus tropicalis. In two of these species, strong skews in expression (mostly female-biased in X. borealis, mostly male-biased in X. tropicalis) are consistent with expectations associated with recombination suppression, and in X. borealis, we hypothesize that a degenerate ancestral Y-chromosome transitioned into its contemporary Z-chromosome. These findings indicate that Xenopus species are tolerant of differences between the sexes in dosage of the products of multiple genes, and offer insights into how evolutionary transformations of ancestral sex chromosomes carry forward to affect the function of new sex chromosomes. This article is part of the theme issue 'Challenging the paradigm in sex chromosome evolution: empirical and theoretical insights with a focus on vertebrates (Part I)'.


Assuntos
Cromossomos Sexuais/genética , Processos de Determinação Sexual , Transcrição Genética , Xenopus/genética , Animais , Feminino , Masculino , Caracteres Sexuais
3.
Int J Biol Macromol ; 185: 620-628, 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34216663

RESUMO

Mandarin (Citrus reticulata L.) essential oil (MEO) reportedly displays excellent antimicrobial properties. In this study, MEO was loaded into chitosan nanoparticles (CSNPs). The characteristics, antibacterial properties and benefit in pork preservation of MEO-CSNPs were evaluated. The MEO-CSNPs displayed an excellent encapsulation efficiency (EE) (67.32%-82.35%), the particle size values of 131.3 nm-161.9 nm, and the absolute zeta potential values above 30 mV. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, and thermogravimetric analysis (TGA) revealed that the MEO was incorporated into CSNPs without requiring a chemical reaction, the antibacterial activity of the MEO remained. Furthermore, the damage of MEO-chitosan nanoemulsions (MEO-CSs) to the cell membranes of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) was confirmed by the change of bacterial cell morphology. The anti-biofilm assays verified that the MEO-CSs substantially inhibited biofilm formation and destroyed the mature biofilms. MEO-CSs were also applied to pork, proving a great potential for pork preservation. This study provides a potential approach for developing and utilizing MEO-CSs as natural antimicrobial agents in the food industry.


Assuntos
Quitosana/química , Citrus/química , Conservação de Alimentos/métodos , Óleos Voláteis/farmacologia , Carne de Porco , Animais , Membrana Externa Bacteriana/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Nanopartículas , Óleos Voláteis/química , Tamanho da Partícula , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/efeitos dos fármacos , Suínos , Termogravimetria , Difração de Raios X
4.
PLoS Genet ; 16(11): e1009121, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33166278

RESUMO

In many species, sexual differentiation is a vital prelude to reproduction, and disruption of this process can have severe fitness effects, including sterility. It is thus interesting that genetic systems governing sexual differentiation vary among-and even within-species. To understand these systems more, we investigated a rare example of a frog with three sex chromosomes: the Western clawed frog, Xenopus tropicalis. We demonstrate that natural populations from the western and eastern edges of Ghana have a young Y chromosome, and that a male-determining factor on this Y chromosome is in a very similar genomic location as a previously known female-determining factor on the W chromosome. Nucleotide polymorphism of expressed transcripts suggests genetic degeneration on the W chromosome, emergence of a new Y chromosome from an ancestral Z chromosome, and natural co-mingling of the W, Z, and Y chromosomes in the same population. Compared to the rest of the genome, a small sex-associated portion of the sex chromosomes has a 50-fold enrichment of transcripts with male-biased expression during early gonadal differentiation. Additionally, X. tropicalis has sex-differences in the rates and genomic locations of recombination events during gametogenesis that are similar to at least two other Xenopus species, which suggests that sex differences in recombination are genus-wide. These findings are consistent with theoretical expectations associated with recombination suppression on sex chromosomes, demonstrate that several characteristics of old and established sex chromosomes (e.g., nucleotide divergence, sex biased expression) can arise well before sex chromosomes become cytogenetically distinguished, and show how these characteristics can have lingering consequences that are carried forward through sex chromosome turnovers.


Assuntos
Cromossomos Sexuais/genética , Processos de Determinação Sexual/genética , Diferenciação Sexual/genética , Xenopus/genética , Animais , Feminino , Aptidão Genética , Gana , Masculino , Recombinação Genética
5.
Molecules ; 25(21)2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33114746

RESUMO

Staphylococcus aureus (S. aureus) creates an array of challenges for the food industry and causes foodborne diseases in people, largely due to its strong antibiotic resistance. Mandarin (Citrus reticulata L.) essential oil (MEO) is recognized as a natural and safe preservative; however, the antibacterial effects and mechanism of MEO to combat S. aureus are not yet clearly understood. This study will examine the inhibitory effects of MEO against S. aureus and explore the antibacterial mechanism thereof from the perspective of membrane destruction. The antibacterial activity of MEO on planktonic S. aureus was examined to determine the minimal inhibitory concentration (MIC). Scanning electron microscope (SEM) images revealed the direct impacts of MEO treatment on the cell structure of S. aureus. The cell membrane was observed to be depolarized, the determination of extracellular nucleic acids, proteins and intracellular adenosine triphosphate (ATP) confirmed the increased permeability of the cell membrane, its integrity was destroyed and the cellular constituents had leaked. These results, thus, provided conclusive evidence that MEO constrains the growth of planktonic S. aureus by affecting the permeability and integrity of its cell membrane. Our study provides a basis for the further development and utilization of MEO as a natural antibacterial agent in the food industry.


Assuntos
Antibacterianos/farmacologia , Citrus/química , Óleos Voláteis/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Potenciais da Membrana/efeitos dos fármacos , Staphylococcus aureus/citologia
6.
Huan Jing Ke Xue ; 41(3): 1456-1465, 2020 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608649

RESUMO

The bacterial community composition in four land-use types was determined and the visualized bacterial network was constructed by 16S rDNA Illumina MiSeq high-throughput sequencing technology and a molecular ecological network method. The results show that Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Actinobacteria, Planctomycetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Firmicutes, Nitrospirae, and Chlorobi are the main bacteria in this area. The number of nodes of urban green land, paddy field, and dry field bacteria networks is higher, and that of natural forest land is lower. The number of connections and average connectivity of dry fields are the highest; following are those of urban green land and paddy field, and those of natural forest land are the lowest. The four bacterial networks are dominated by positive correlation, and the ratio of competition relationship is TL > LD > HT > ST. The average network path and modularity of the soil bacteria networks of paddy field and dry land are small, while the average connectivity and clustering coefficient are higher. Some flora of Acidobacteria, Firmicutes, and Proteobacteria play an important role in the soil bacterial network in this area. The classification of operational taxonomic units is different among the key nodes of different bacterial molecular ecological networks, and there is almost no overlap. The relative abundance of bacteria of some key nodes in the four bacterial networks is low (<1%), and these are not the main bacteria in this area. The soil microflora in dry land are mainly affected by TP (P<0.05), the soil microflora in paddy field were mainly affected by clay, silt, and water content (P<0.05), and that in natural forest land and urban green land were mainly affected by C/N (P<0.05). The above results show that different land-use patterns lead to changes in soil physical and chemical properties and the interaction between soil bacteria species. The bacterial network of dry land soil is larger and the relationship between species is more complex. The bacteria in different land-use types are mainly cooperative, and the competition is weak. Compared with other land-use types, there is stronger competition between the bacteria in natural forest soil. The soil bacteria in paddy field and dry land are the most sensitive to the external environment, respond more quickly, and the community structure is easier to change. The response of soil bacteria in natural forest land and urban green land is slower, and the disturbance of environmental factors does not affect the whole bacterial ecological network in a short time, and thus the community structure is more stable. Some bacteria have the phenomenon of species role transformation between networks. The abundance and community distribution of microorganisms cannot indicate the strength of their connectivity between network nodes; low-abundance bacteria in soil play an important role in the construction of bacterial networks.


Assuntos
Microbiologia do Solo , Solo , Acidobacteria , Florestas , RNA Ribossômico 16S
7.
Mol Biol Evol ; 37(3): 799-810, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31710681

RESUMO

Phenotypic invariance-the outcome of purifying selection-is a hallmark of biological importance. However, invariant phenotypes might be controlled by diverged genetic systems in different species. Here, we explore how an important and invariant phenotype-the development of sexually differentiated individuals-is controlled in over two dozen species in the frog family Pipidae. We uncovered evidence in different species for 1) an ancestral W chromosome that is not found in many females and is found in some males, 2) independent losses and 3) autosomal segregation of this W chromosome, 4) changes in male versus female heterogamy, and 5) substantial variation among species in recombination suppression on sex chromosomes. We further provide evidence of, and evolutionary context for, the origins of at least seven distinct systems for regulating sex determination among three closely related genera. These systems are distinct in their genomic locations, evolutionary origins, and/or male versus female heterogamy. Our findings demonstrate that the developmental control of sexual differentiation changed via loss, sidelining, and empowerment of a mechanistically influential gene, and offer insights into novel factors that impinge on the diverse evolutionary fates of sex chromosomes.


Assuntos
Pipidae/fisiologia , Cromossomos Sexuais/genética , Animais , Evolução Biológica , Evolução Molecular , Feminino , Deriva Genética , Masculino , Fenótipo , Pipidae/genética , Recombinação Genética , Seleção Genética , Processos de Determinação Sexual , Diferenciação Sexual
8.
J Sep Sci ; 42(15): 2471-2481, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31012280

RESUMO

Overcoming epidermal growth factor receptor resistance is a critical problem that needs to be solved in clinical practice. Drugs that downregulate the fatty acid synthase-epidermal growth factor receptor will become novel treatments for non-small cell lung cancer. Solanum nigrum, extracted with water at 4°C, shows strong cytotoxic activity and inhibits tumor growth in Lewis tumor bearing-mice in a dose-dependent manner. A novel active compound in S. nigrum, solaoiacid, was successfully separated and purified from S. nigrum by preparative high-performance liquid chromatography with mass spectrometry and ultra high performance liquid chromatography with time-of-flight tandem mass spectrometry. The IC50 of solaoiacid on lung cancer cells was 2.3 µmol/L, which was significantly lower than that of the known steroidal glycoalkaloid. Label-free proteomics and STRING Network analysis were used to identify significantly deregulated proteins in lung cancer cells that were treated with the fresh ripe fruit extracts of S. nigrum. S. nigrum regulates multiple signal pathways, including the epidermal growth factor receptor pathway. S. nigrum downregulated 24 main proteins with direct roles in fatty acid biosynthesis. Both S. nigrum and solaoiacid showed strong downregulation of the fatty acid synthase-epidermal growth factor receptor and anti-non-small cell lung cancer effects, and thus will become a novel drug for the treatment of non-small cell lung cancer.


Assuntos
Alcaloides/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Extratos Vegetais/farmacologia , Solanum nigrum/química , Esteroides/farmacologia , Células A549 , Alcaloides/química , Alcaloides/isolamento & purificação , Animais , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Carcinoma Pulmonar de Células não Pequenas/patologia , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Regulação para Baixo/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Receptores ErbB/antagonistas & inibidores , Receptores ErbB/metabolismo , Frutas/química , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Masculino , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos C57BL , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Experimentais/metabolismo , Neoplasias Experimentais/patologia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Esteroides/química , Esteroides/isolamento & purificação
9.
Acta Biochim Biophys Sin (Shanghai) ; 50(11): 1158-1165, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30260364

RESUMO

The cluster of differentiation 81 (CD81), a member of the transmembrane 4 superfamily, is primarily found to be expressed in a wide variety of cells including T and B cells of vertebrates as a critical modulator. In the present study, the open reading frame of a CD81 gene homolog (Lja-CD81) was cloned in lamprey, Lampetra japonica, which is 702 bp long and encodes a protein of 233-amino acids. Although Lja-CD81 seems to be close to CD9 molecules in their full-length sequences, Lja-CD81 possesses higher identity to vertebrates' CD81 than to CD9 (including a lamprey CD9) molecules in their large extracellular loops. In addition, it also possesses a myristoylation site (Met-Gly-Val-Glu-Gly-Cys-Leu-Lys) in its N-terminal region which is identical to the N-terminal regions of CD81 molecules. These data suggest that CD9 and CD81 molecules diverged no later than the emergence of jawless vertebrates. The mRNA levels of Lja-CD81 in lymphocytes and supraneural myeloid bodies were up-regulated significantly after stimulation with mixed antigens, and a similar expressional pattern of Lja-CD81 at protein level was also confirmed. Furthermore, Lja-CD81 was found to be co-localized with variable lymphocyte receptor B (VLRB) evenly on the cell membrane of peripheral blood lymphocytes isolated from control group, but they were found to aggregate on one side of the membrane of peripheral blood VLRB+ lymphocytes after stimulation with mixed antigens. All these results indicate that the Lja-CD81 identified in lamprey may play an important role in the immune response of lamprey VLRB+ lymphocytes.


Assuntos
Proteínas de Peixes/imunologia , Lampreias/imunologia , Linfócitos/imunologia , Receptores de Antígenos/imunologia , Tetraspanina 28/imunologia , Sequência de Aminoácidos , Animais , Western Blotting , Clonagem Molecular , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Perfilação da Expressão Gênica , Lampreias/genética , Lampreias/metabolismo , Linfócitos/metabolismo , Microscopia Confocal , Receptores de Antígenos/genética , Receptores de Antígenos/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Tetraspanina 28/genética , Tetraspanina 28/metabolismo
10.
Huan Jing Ke Xue ; 39(1): 379-388, 2018 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965705

RESUMO

Topsoil (0-20 cm) samples (n=101) in 5 different land use types in Shenyang North New Area (SNNA), Shenyang, China were collected using the uniform grid layout method to investigate the spatial distribution characteristics, composition spectrum, and source analysis of 16 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants by the Environmental Protection Agency of the United States. Results showed that the total concentration of the 16 PAHs (ΣPAHs) in soils of SNNA ranged from 123.7 µg·kg-1 to 932.5 µg·kg-1. The PAH components were mainly dominated by 3-ring and 4-ring PAHs, of which the proportion of 3-ring PAHs was the highest. The spatial distribution of the ΣPAHs concentration was obvious, showing a decreasing tendency from south to north and from east to west. In the five soil types, the average concentrations of the ΣPAHs were relatively higher in the urban green space and the artificial forest, followed by the vegetable land, while the total PAH concentrations in paddy fields and corn fields were relatively lower and had no obvious spatial distribution differences. Source apportionment results studied using characteristic ratio analysis and factor analysis/multivariate linear regression showed that the main sources of PAHs in the topsoil of SNNA were mixed sources. Industrial coal combustion and motor vehicle exhaust were the main PAH contributors, with a combined contribution rate of 79.6%. The oil spill and coke oven contribution rate was about 16.2%, and the biomass fuel combustion was about 4.2%.

11.
Mol Carcinog ; 57(2): 193-200, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28976622

RESUMO

Autophagy is an evolutionarily conserved pathway in which cytoplasmic contents are degraded and recycled. This study found that submicromolar concentrations of urolithin A, a major polyphenol metabolite, induced autophagy in SW620 colorectal cancer (CRC) cells. Exposure to urolithin A also dose-dependently decreased cell proliferation, delayed cell migration, and decreased matrix metalloproteinas-9 (MMP-9) activity. In addition, inhibition of autophagy by Atg5-siRNA, caspases by Z-VAD-FMK suppressed urolithin A-stimulated cell death and anti-metastatic effects. Micromolar urolithin A concentrations induced both autophagy and apoptosis. Urolithin A suppressed cell cycle progression and inhibited DNA synthesis. These results suggest that dietary consumption of urolithin A could induce autophagy and inhibit human CRC cell metastasis. Urolithins may thus contribute to CRC treatment and offer an alternative or adjunct chemotherapeutic agent to combat this disease.


Assuntos
Autofagia/efeitos dos fármacos , Neoplasias Colorretais/tratamento farmacológico , Cumarínicos/farmacologia , Taninos Hidrolisáveis/farmacologia , Apoptose/efeitos dos fármacos , Caspases/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Neoplasias Colorretais/metabolismo , Humanos , Metaloproteinase 9 da Matriz/metabolismo , RNA Interferente Pequeno/metabolismo
12.
Front Cell Neurosci ; 11: 171, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28680394

RESUMO

Although development of cognitive decline in cancer patients who receive chemotherapy is common, the underlying mechanism(s) remains to be identified. As abnormalities in adult hippocampal neurogenesis may serve as substrate for cognitive dysfunction, the present study examines the effect of cyclophosphamide (CPP), a widely prescribed chemotherapeutic agent, on dendritic development of adult-born hippocampal granule cells in the rat. CPP was intraperitoneally injected into male Sprague-Dawley rats once a week for four consecutive weeks. Four weeks and 1 week after the last dose of CPP, Morris water maze test and doublecortin (DCX) immunohistochemistry were carried out to determine the effects of CPP on cognitive function and the rate of hippocampal neurogenesis, respectively. Adult newborn hippocampal granule cells were labeled at the same day as the first dose of CPP and were examined 10 weeks after labeling. Results showed that cognitive decline induced by CPP was associated with both suppressed adult hippocampal neurogenesis and abnormal development of dendrites of newborn granule cells. The abnormalities of dendrites in newborn granule cells after CPP exposure included less dendritic branching, shorter total dendritic length, thinner and torturous dendritic shafts with intermittent appearances of varicosities, and lower spine densities of stubby and thin types along dendritic shafts, but an increased density of mushroom-like spines. Adult-born granule cells in the presence of CPP, a widely used anti-cancer medication, display abnormal dendritic morphologies and fewer dendritic spines which may underlie cognitive dysfunction.

13.
Front Microbiol ; 8: 988, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28638370

RESUMO

Biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (EPS), are difficult to remove in food production facilities. In this study, the use of acidic electrolyzed water (AEW) to remove foodborne pathogen biofilms was evaluated. We used a green fluorescent protein-tagged Escherichia coli for monitoring the efficiency of AEW for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm disappeared rapidly and the population of biofilm cells was reduced by more than 67%. Additionally, AEW triggered EPS disruption, as indicated by the deformation of the carbohydrate C-O-C bond and deformation of the aromatic rings in the amino acids tyrosine and phenylalanine. These deformations were identified by EPS chemical analysis and Raman spectroscopic analysis. Scanning electron microscopy (SEM) images confirmed that the breakup and detachment of biofilm were enhanced after AEW treatment. Further, AEW also eradicated biofilms formed by both Gram-negative bacteria (Vibrio parahaemolyticus) and Gram-positive bacteria (Listeria monocytogenes) and was observed to inactivate the detached cells which are a potential source of secondary pollution. This study demonstrates that AEW could be a reliable foodborne pathogen biofilm disrupter and an eco-friendly alternative to sanitizers traditionally used in the food industry.

14.
Ann Thorac Surg ; 104(3): 841-846, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28366463

RESUMO

BACKGROUND: In recent years, minimally invasive transthoracic device closure has been introduced as an alternative treatment option for selected patients with juxtaarterial ventricular septal defects. This study evaluated the midterm safety and efficacy of using device closure in selected patients. METHODS: Between January 2008 and December 2014, 25 patients with juxtaarterial ventricular septal defects who met the inclusion criteria were enrolled in this study. Periventricular closure was attempted using minimally invasive transthoracic device closure without cardiopulmonary bypass under general anesthesia and transesophageal echocardiography guidance. Patients were strictly monitored according to a standard protocol by one specially appointed doctor. RESULTS: Minimally invasive transthoracic device closure was successfully performed in 23 patients (92%) with a median age of 18 months. Device closure failed in 2 patients (1 with aortic regurgitation and 1 with right ventricular outflow tract stenosis), and they were converted to an open operation. No severe complications (device shift, significant arrhythmia, ventricular outflow tract obstruction, or obvious valve regurgitation) were observed. There was no closure-associated valve regurgitation. No patient had worrisome progression of aortic regurgitation or pulmonary regurgitation. CONCLUSIONS: In select patients, minimally invasive transthoracic device closure of juxtaarterial ventricular septal defects appears to be safe and effective, with good midterm outcomes.


Assuntos
Procedimentos Cirúrgicos Cardíacos/instrumentação , Comunicação Interventricular/cirurgia , Ventrículos do Coração/cirurgia , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Dispositivo para Oclusão Septal , Adolescente , Criança , Pré-Escolar , Ecocardiografia Transesofagiana , Feminino , Seguimentos , Comunicação Interventricular/diagnóstico , Ventrículos do Coração/diagnóstico por imagem , Humanos , Lactente , Masculino , Desenho de Prótese , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
15.
Cancer Cell Int ; 16: 40, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27252596

RESUMO

[This retracts the article DOI: 10.1186/s12935-014-0154-0.].

16.
Dev Genes Evol ; 226(2): 87-98, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26935717

RESUMO

CD82, a member of the tetraspanins, is originally identified as an accessory molecule in T cell activation, and it participates in the formation of immune synapse both in T cells and antigen-presenting cells of jawed vertebrates. In the present study, a CD82 homologous complementary DNA (cDNA) sequence is identified in the lamprey Lampetra japonica. The open reading frame of this sequence is 801 bp long and encodes a 266-amino acid protein. The multialignment of this sequence with several typical CD82s and CD37s of jawed vertebrates shows that it also possesses their conserved four transmembrane domains and a six-cysteine motif Cys-Cys-Gly…Cys-Ser-Cys…Cys…Cys, which is a characteristic motif of CD82 and CD37 vertebrate tetraspanin sequences. Since it is close to CD82s in sequence similarity, we name it as Lja-CD82-like. From the distribution profile of the conserved motifs of CD82-like, CD82, and CD37 molecules from molluscas to mammals, it seems that the CD82s and CD37s evolved from a common ancestral gene through a gene duplication event to their modern forms by a short insertion or substitution approaches. The phylogenetic analysis indicated that CD82 and CD37 molecules of jawed vertebrates originated from a common ancestral gene which is close to agnathan CD82-like and evolved into two distinct paralogous groups maybe after the divergence of jawed and jawless vertebrates. An expression vector with trigger factor (TF) was constructed to ensure that Lja-CD82-like express in prokaryotic expression host. The expressions of Lja-CD82-like messenger RNA (mRNA) and protein in immune-related tissues of lamprey were detected by real-time quantitative polymerase chain reaction and western blotting. Results showed that the mRNA and the protein levels of Lja-CD82-like were significantly upregulated in lymphocyte-like cells, gills, and supraneural myeloid bodies after stimulation with mixed antigens, respectively. Our data provided a foundation for the further study of Lja-CD82-like and its role in immune response process of jawless vertebrates.


Assuntos
Evolução Molecular , Proteínas de Peixes/genética , Lampreias/genética , Tetraspaninas/genética , Motivos de Aminoácidos , Animais , Clonagem Molecular , Sequência Conservada , Proteínas de Peixes/química , Humanos , Proteína Kangai-1/química , Proteína Kangai-1/genética , Linfócitos , Filogenia , Homologia de Sequência de Aminoácidos , Tetraspaninas/química , Transcriptoma
17.
Gene ; 579(2): 193-200, 2016 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-26743129

RESUMO

The non-receptor protein tyrosine kinase (nrPTK) Fyn, a member of the avian sarcoma virus transforming gene (Src) kinase family, plays a very significant role in cell growth, survival, apoptosis, tumor formation and immune response. In this study, a homolog of nrPTK Fyn was identified for the first time in the lamprey, Lampetra japonica and was named "Lja-Fyn". The cDNA fragment of lamprey lja-fyn contains a 1611-bp open reading frame, which encodes a protein of 537 amino acids. Multiple sequence alignment analysis showed that it shares four conserved domains (Src homology (SH) 4, SH3, SH2 and protein kinases catalytic domains) and a variable unique domain with vertebrates Fyn molecules. Though Lja-Fyn has high sequence similarity with typical Fyn and Yes molecules of jawed vertebrates, the identities among Lja-Fyn and typical Fyn molecules in unique domain are relatively higher than that among Lja-Fyn and typical Yes molecules. The result indicates that Lja-Fyn is a homolog of Fyn rather than Yes. The phylogenetic analysis showed that Fyn, Yes and Src molecules are grouped into three distinct phylogenetic clusters, and Lja-Fyn is grouped as a single branch in Fyn cluster. The real-time quantitative PCR assay revealed the wide distribution of the lja-fyn mRNA in lamprey immune related tissues. After stimulation with mixed antigens, the levels of lja-fyn mRNA were obviously up-regulated in the gill and lymphocyte-like cells, and the similar results were got by western blot analysis of Lja-Fyn protein expression. These results indicated that nrPTK Lja-Fyn was likely to be involved in immune response. Furthermore, our present findings also provide the necessary information for understanding the distinction between lamprey Lja-Fyn and other members of jawed vertebrates in Src family.


Assuntos
Imunidade Inata/genética , Lampreias/genética , Proteínas Proto-Oncogênicas c-fyn/genética , Animais , Lampreias/imunologia , Proteínas Proto-Oncogênicas c-fyn/imunologia , Homologia de Sequência de Aminoácidos
18.
Environ Sci Pollut Res Int ; 23(5): 4868-75, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26545889

RESUMO

To improve the denitrification characteristics of anaerobic denitrifying bacteria and obviate the disadvantage of use of explosive hydrogen gas, tourmaline, a polar mineral, was added to the hydrogenotrophic denitrification system in this study. Microbial reduction of nitrate in the presence of tourmaline was evaluated to assess the promotion effect of tourmaline on nitrate biodegradation. The experiment results demonstrated that tourmaline speeded up the cultivation process of bacteria from 65 to 36 days. After domestication of the bacteria, nitrate (50 mg NO3 (-)-N L(-1)) was completely removed within 3 days in the combined tourmaline-bacteria system, and the generated nitrite was also removed within 8 days. The reduction rate in this system is higher relative to that in the bacteria system alone. Efficient removal of nitrate by tourmaline-supported anaerobic bacteria (without external hydrogen input) indicated that tourmaline might act as the sole hydrogen donor to sustain autotrophic denitrification. Besides the production of hydrogen, the promoted activity of anaerobic denitrifying bacteria might be caused by the change of water properties, e.g., the pH of aqueous solutions was altered to about 8.0 and the oxidation-reduction potential decreased by 62 % in the tourmaline system. The distinctive effects of tourmaline on bacteria were related to its electric properties.


Assuntos
Bactérias Anaeróbias/metabolismo , Reatores Biológicos/microbiologia , Minerais/química , Nitratos/análise , Poluentes Químicos da Água/análise , Processos Autotróficos , Biodegradação Ambiental , Compostos de Boro/química , Desnitrificação , Transporte de Elétrons , Água Subterrânea/química , Hidrogênio/química , Concentração de Íons de Hidrogênio , Nanopartículas , Tamanho da Partícula
19.
Yi Chuan ; 37(11): 1149-59, 2015 11.
Artigo em Chinês | MEDLINE | ID: mdl-26582529

RESUMO

In recent years, the antigen recognition mechanism based on variable lymphocyte receptors (VLRs) was found in agnathan lamprey. To illuminate the genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of lamprey and explore the evolutionary relationship of adaptive immune responses between the jawless and jawed vertebrates, we constructed cDNA libraries of lamprey (Lampetra japonica) gills before and after stimulation, and then performed high-throughput transcriptome sequencing and analysis. Through functional annotation of 88 525 assembled unigenes, 21 704 and 9769 unigenes were annotated in Gene Ontology (GO) and Kyto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. Among 999 unigenes involved in multiple pathways of immune system, 184 unigenes were highly homologous to 51 TCR (T cell receptor) and BCR (B cell receptor) signalling molecules in higher vertebrates, indicating that molecules involved in adaptive immune signalling pathways in higher vertebrates also exist in lampreys. In addition, identification of five VLRA, seven VLRB and four VLRC molecules suggest that at least three types of lymphocyte subsets are distributed in lamprey gill mucosal immune tissues. The results of real-time fluorescence quantitative PCR showed that the expression levels of Lck, Fyn and Zap70 were up-regulated after immune stimulation while those of Syk, Btk and Blnk were not changed significantly, indicating the activation of TCR-like signal transduction pathway after antigen stimulation in lamprey gill tissues. Our studies preliminaryly proved that two parallel adaptive immune systems in jawless and jawed vertebrates have common genetic basis, and also provided valuable clues to the exploration of signalling processes of VLRA⁺, VLRB⁺, and VLRC⁺ lymphocyte-like cells in response to antigens.


Assuntos
Imunidade nas Mucosas/genética , Lampreias/imunologia , Linfócitos/imunologia , Imunidade Adaptativa , Animais , Bases de Dados Genéticas , Biblioteca Gênica , Sequenciamento de Nucleotídeos em Larga Escala
20.
Front Cell Neurosci ; 9: 384, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26500490

RESUMO

To understand the potential role of enhanced hippocampal neurogenesis after pilocarpine-induced status epilepticus (SE) in the development of epilepsy, we quantitatively analyzed the geometry of apical dendrites, synaptic transmission, and activation levels of normotopically distributed mature newborn granule cells in the rat. SE in male Sprague-Dawley rats (between 6 and 7 weeks old) lasting for more than 2 h was induced by an intraperitoneal injection of pilocarpine. The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling. Mature granule cells born after SE had dendritic complexity similar to that of granule cells born naturally, but with denser mushroom-like spines in dendritic segments located in the outer molecular layer. Miniature inhibitory post-synaptic currents (mIPSCs) were similar between the controls and rats subjected to SE; however, smaller miniature excitatory post-synaptic current (mEPSC) amplitude with a trend toward less frequent was found in mature granule cells born after SE. After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells. Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

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