Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 21
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Molecules ; 25(4)2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32093422

RESUMO

Edible mushrooms are an important source of nutraceuticals and for the discovery of bioactive metabolites as pharmaceuticals. In this work, the OSMAC (One Strain, Many Active Compounds) approach was used to isolate two new compounds (1 and 2) along with seven known compounds (3-9) from a mycelial culture of a unique North American edible mushroom Hericium sp. The fruiting body was collected in Marine on St. Croix, Minnesota (USA), and mycelial cultures were grown on four different solid and liquid media. Extracts from the mycelial cultures were screened for antimicrobial activity and only the extract from the Cheerios substrate culture exhibited antifungal activity. Bioassay guided fractionation and HPLC analysis were used to isolate nine pure compounds and the structures of the known compounds were established by analysis of the NMR and mass spectrometry data and comparison to published reports. Compound 1 is a new erinacerin alkaloid and 2 is an aldehyde derivative of 4-hydroxy chroman. Four chlorinated orcinol derivatives (3-6), a pyran (7), erinaceolactone (8), and erinacine (9) were identified. Compound 4 showed antifungal activity against C. albicans and C. neoformans (MIC = 31.3-62.5 µg/mL, respectively). Compound 4 also inhibited biofilm formation of C. albicans and C. neoformans at 7.8 µg/mL. These results suggest that mycelial cultures of edible fungi may provide useful, bioactive compounds.

2.
Food Sci Nutr ; 7(9): 3062-3070, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31572599

RESUMO

Seedpod, the nonedible portion of lotus (Nelumbo nucifera Gaertn.), was reported to be rich in polyphenols. The objective of this study was to investigate the major bioactive polyphenols of the lotus seedpods. The total polyphenol content (TPC) from ethanol extract of lotus seedpod (PELS) was found to be 34.23 µg gallic acid equivalents (GAE)/mg extract. Four polyphenolic compounds were identified in the PELS, comprised of one flavan-3-ol (catechin) and three flavonoids (kaemferol, quercetin and hyperoside). In vitro antioxidant and antiproliferative properties of the PELS were evaluated. PELS exhibited 89.38%, 99.82%, 68.25%, and 95.82% scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, hydroxyl, and 2,2'azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals, respectively, at 1.6 mg/ml. The Fe3+ reducing power of PELS was 0.605 at 0.32 mg/ml, which is comparable to glutathione (GSH). The PELS showed 31.79% metal chelating capacity and 87.79% inhibition of linoleic acid auto-oxidation at 1.6 mg/ml. PELS showed cytotoxicity toward HepG2 and LNcap cell lines in vitro with IC50 values at 44.59 and 11.50 µg/ml, respectively. The findings of this study provide evidences that the inedible lotus seedpod could be a source for natural antioxidants and anticancer agents.

3.
PeerJ ; 7: e7705, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31598423

RESUMO

Background: Esophageal squamous cell carcinoma (ESCC) is a subtype of esophageal cancer with high incidence and mortality. Due to the poor 5-year survival rates of patients with ESCC, exploring novel diagnostic markers for early ESCC is emergent. Collagen, the abundant constituent of extracellular matrix, plays a critical role in tumor growth and epithelial-mesenchymal transition. However, the clinical significance of collagen genes in ESCC has been rarely studied. In this work, we systematically analyzed the gene expression of whole collagen family in ESCC, aiming to search for ideal biomarkers. Methods: Clinical data and gene expression profiles of ESCC patients were collected from The Cancer Genome Atlas and the gene expression omnibus databases. Bioinformatics methods, including differential expression analysis, survival analysis, gene sets enrichment analysis (GSEA) and co-expression network analysis, were performed to investigate the correlation between the expression patterns of 44 collagen family genes and the development of ESCC. Results: A total of 22 genes of collagen family were identified as differentially expressed genes in both the two datasets. Among them, COL1A1, COL10A1 and COL11A1 were particularly up-regulated in ESCC tissues compared to normal controls, while COL4A4, COL6A5 and COL14A1 were notably down-regulated. Besides, patients with low COL6A5 expression or high COL18A1 expression showed poor survival. In addition, a 7-gene prediction model was established based on collagen gene expression to predict patient survival, which had better predictive accuracy than the tumor-node-metastasis staging based model. Finally, GSEA results suggested that collagen genes might be tightly associated with PI3K/Akt/mTOR pathway, p53 pathway, apoptosis, cell cycle, etc. Conclusion: Several collagen genes could be potential diagnostic and prognostic biomarkers for ESCC. Moreover, a novel 7-gene prediction model is probably useful for predicting survival outcomes of ESCC patients. These findings may facilitate early detection of ESCC and help improves prognosis of the patients.

4.
Biomed Pharmacother ; 111: 579-587, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30599319

RESUMO

Dietary phenols are antioxidants with diverse physiological functions that are beneficial for human health. The objective of this research work was to investigate antioxidant activity of p-coumaric acid (p-CA) using four in vitro methods, the protective effects against oxidative stress in PC12 cells, and hypolipidemic effects on High fat-diet (HFD) mice model. The p-CA exhibited moderate antioxidant activity in the selected in vitro assay. The highest chelating activity of p-CA at 50 µg/mL was found to be 52.22%. Pretreatment with p-CA significantly enhanced cell viability of PC12 cell and suppressed AAPH-induced intracellular ROS generation and AAPH-induced LDH release. The hypolipidemic effects of p-CA (100 mg/kg BW) was directly linked to the increased expression of nuclear factor erythroid 2-related factor (Nrf2) by 2.0-fold, Glutathione peroxidase (Gpx) by 3.8-fold, Superoxide dismutase (SOD-1) by 1.6-fold, Heme oxygenase (HO-1) by 1.72-fold and NAD(P)H Quinone Dehydrogenase 1 (NQO-1) by 1.5-fold compared with HFD group. In addition to these effects, p-CA decreased total cholesterol and atherosclerosis index levels, and increased catalase (CAT) level in serum, total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) levels in liver as compared HFD group. Administration of p-CA also promoted the recovery of hyperlipidemia steatohepatitis in mice by ameliorating lipid peroxidation. These results suggested that p-CA is a potent antioxidant with potential therapeutic efficacy for treating hyperlipidemia symptoms.


Assuntos
Antioxidantes/uso terapêutico , Hiperlipidemias/prevenção & controle , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Propionatos/uso terapêutico , Animais , Antioxidantes/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Dieta Hiperlipídica/efeitos adversos , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos/métodos , Depuradores de Radicais Livres/farmacologia , Depuradores de Radicais Livres/uso terapêutico , Hiperlipidemias/metabolismo , Peroxidação de Lipídeos/fisiologia , Masculino , Camundongos , Estresse Oxidativo/fisiologia , Células PC12 , Propionatos/farmacologia , Ratos , Resultado do Tratamento
5.
Molecules ; 23(12)2018 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-30563201

RESUMO

The purpose of this research was to investigate the chemical profile, nutritional quality, antioxidant and hypolipidemic effects of Mexican chia seed oil (CSO) in vitro. Chemical characterization of CSO indicated the content of α-linolenic acid (63.64% of total fatty acids) to be the highest, followed by linoleic acid (19.84%), and saturated fatty acid (less than 11%). Trilinolenin content (53.44% of total triacylglycerols (TAGs)) was found to be the highest among seven TAGs in CSO. The antioxidant capacity of CSO, evaluated with ABTS•+ and DPPH• methods, showed mild antioxidant capacity when compared with Tocopherol and Catechin. In addition, CSO was found to lower triglyceride (TG) and low-density lipoprotein-cholesterol (LDL-C) levels by 25.8% and 72.9%respectively in a HepG2 lipid accumulation model. As CSO exhibits these chemical and biological characteristics, it is a potential resource of essential fatty acids for human use.


Assuntos
Compostos Fitoquímicos/química , Óleos Vegetais/química , Salvia/química , Antioxidantes/química , Ácidos Graxos/química , Células Hep G2 , Humanos , Compostos Fitoquímicos/metabolismo , Óleos Vegetais/metabolismo , Sementes/química , Triglicerídeos , Ácido alfa-Linoleico/química
6.
Biomed Pharmacother ; 108: 558-564, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30243089

RESUMO

The treatment of dermatophytosis has improved considerably over the past several decades following the introduction of the oral antifungals such as azoles and amphotericin B. However, these drugs have had limited success because the treated fungi often develop drug resistance, resulting in recurrence when applied in various topical formulations. Thus, there are constant needs for new topical agents that are effective against dermatophytosis. Dipyrithione is an attractive candidate to become an antifungal agent due to its broad spectrum of antimicrobial activities. In this study, we determined that dipyrithione could potently inhibit the growth of Trichophyton rubrum, which is the most common cause of dermatophytosis. The MIC50 value of dipyrithione against T. rubrum was measured as 6.03 µM, as compared with miconazole (MIC50: 1.38 µM). Additionally, the compound caused morphological changes in the fungi, which was examined using the morphological interference assay. The in vivo experiment further revealed that dipyrithione had a healing effect on the skin of guinea pigs infected with T. rubrum. Our studies have demonstrated that dipyrithione had a potent antifungal activity in vitro and in vivo, suggesting that it could be formulated as a potential antifungal lead compound in search for novel therapeutic agents against dermatophytosis.


Assuntos
Antifúngicos/farmacologia , Piridinas/farmacologia , Tinha/tratamento farmacológico , Trichophyton/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Feminino , Cobaias , Testes de Sensibilidade Microbiana , Pele/efeitos dos fármacos , Pele/microbiologia , Tinha/microbiologia
7.
Food Chem Toxicol ; 119: 50-60, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29772268

RESUMO

Pyrrolizidine alkaloids (PAs) are secondary metabolites of plants. PAs have been reported to be hepatotoxic, mutagenic, and carcinogenic; they are a significant group of natural toxins affecting livestock, wildlife, and humans. To date, over 10,000 PAs poisoning cases have been reported worldwide. In recent years, many articles have reported the detection of PAs in various foods, including honey, milk, meat, eggs, tea and salad. This review summarized the contamination of PAs in foods, state of the art detection methods and regulations by different countries and authorities, hoping to propose effective solutions to minimize the consumption of PAs in food.


Assuntos
Análise de Alimentos , Alcaloides de Pirrolizidina/química , Alcaloides de Pirrolizidina/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Contaminação de Alimentos , Humanos
8.
J Ethnopharmacol ; 191: 372-378, 2016 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-27340103

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Leigong Mountain is an area in the Southwest of China where there is a high incidence rate of athlete's foot, but the Miao people, a Chinese minority who reside in this mountainous area have suffered less from this disease due to their use of the herbal medicine Isodon flavidus (Hand.-Mazz.) H. Hara. AIM OF THE STUDY: The present study is to identify the active chemical constituents responsible for antifungal effects of the folk medicine plant. MATERIALS AND METHODS: The natural compounds were separated from the methanol extract of the twigs and leaves of I. flavidus by phytochemical study using chromatographic methods, and their chemical structures were determined by analysis of the spectroscopic data including 1D and 2D NMR spectra. The absolute configuration of fladin A (1) was further confirmed by X-ray crystallographic analysis. The compounds were evaluated for their antifungal activity against the athlete's foot fungus Trichophyton rubrum. They were further evaluated for their antimicrobial and anti-biofilm activity against the dental pathogens Streptococcus mutans, Porphyromonas gingivalis and Candida albicans. RESULTS: Phytochemical and biological studies of I. flavidus led to the discovery of two antifungal compounds, fladin A (1) and lophanic acid (2). Fladin A (1) is a novel diterpene with an unprecedented cyclic ether group formed between C-4 and C-9. Lophanic acid (2) displayed inhibition activity against the athlete's foot fungus Trichophyton rubrum with an MIC value of 7.8µg/mL, and fladin A (1) also showed inhibition activity against the fungus with a MIC value of 62.5µg/mL. CONCLUSIONS: Our identification of two antifungal compounds provided strong evidence for the Miao people to use I. flavidus as a medicinal plant for treatment of athlete's foot disease. The very different chemical structures of the active compounds from those in the market presents them as potential antifungal lead compounds for follow-up study.


Assuntos
Antifúngicos/farmacologia , Isodon/química , Extratos Vegetais/farmacologia , Tinha dos Pés/tratamento farmacológico , Trichophyton/efeitos dos fármacos , Antifúngicos/química , Antifúngicos/isolamento & purificação , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Cristalografia por Raios X , Metanol/química , Testes de Sensibilidade Microbiana , Estrutura Molecular , Fitoterapia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Caules de Planta/química , Plantas Medicinais , Espectroscopia de Prótons por Ressonância Magnética , Solventes/química , Tinha dos Pés/microbiologia , Trichophyton/crescimento & desenvolvimento
9.
Food Funct ; 7(7): 3017-30, 2016 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-27326537

RESUMO

There are many herbal teas that are found in nature that may be effective at treating the symptoms and also shortening the duration of viral infections. When combating viral infections, T lymphocytes are an indispensable part of human acquired immunity. However, studies on the use of natural products in stimulating lymphocyte-mediated interferon-gamma (IFN-γ) production are very limited. In this study, we found that acteoside, a natural phenylpropanoid glycoside from Kuding Tea, enhanced IFN-γ production in mouse lymphocytes in a dose-dependent manner, particularly in the CD4+ and CD8+ subsets of T lymphocytes. To this end, we suggest that the antiviral activity of acteoside was highly correlated to its inducing ability of IFN-γ production. Mechanistically, the activation of T-bet enhanced the promoter of IFN-γ and subsequently resulted in an increased IFN-γ production in T cells. Collectively, we have found a natural product with the capacity to selectively enhance mouse T cell IFN-γ production. Given the role of IFN-γ in the immune system, further studies to clarify the role of acteoside in inducing IFN-γ and prevention of viral infection are needed.


Assuntos
Antivirais/farmacologia , Produtos Biológicos/farmacologia , Glucosídeos/farmacologia , Interferon gama/metabolismo , Fenóis/farmacologia , Animais , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Relação Dose-Resposta Imunológica , Glicosídeos/farmacologia , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Ativação Linfocitária/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas , Células RAW 264.7 , Proteínas com Domínio T/metabolismo , Linfócitos T/efeitos dos fármacos , Linfócitos T/metabolismo , Chás de Ervas/análise
10.
Phytomedicine ; 23(6): 641-53, 2016 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-27161405

RESUMO

BACKGROUND: Catharanthus roseus (L.) G. Don consists of a range of dimeric indole alkaloids with significant antitumor activities. These alkaloids have been found to possess apoptosis-inducing activity against tumor cells in vitro and in vivo mediated by nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and c-Jun N-terminal kinase (JNK) pathways, in which DNA damage and mitochondrial dysfunction play important roles. In this study, a unique bisindole alkaloid named cathachunine, along with five known dimeric indole alkaloids, was obtained from C. roseus and investigated in vitro. PURPOSE: The aim of this study was to investigate the antitumor activity of isolated alkaloids and the mechanism through which cathachunine exerts its antitumor effect. STUDY DESIGN AND METHODS: Cell growth inhibition was assessed by WST-1 and lactate dehydrogenase (LDH) assays in HL60, K562 leukemia cells and EA.hy926 umbilical vein cells. Induction of apoptosis in HL60 cells was confirmed by observation of nuclear morphology, a caspase-3 activity assay and annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) double staining. The intrinsic apoptotic pathway induced by cathachunine was evidenced by B-cell lymphoma 2/Bcl-2-associated X protein (Bcl-2/Bax) dysregulation, loss of mitochondrial membrane potential, translocation of cytochrome c, and cleavage of caspase-3 and poly-ADP ribose polymerase (PARP). Reactive oxygen species (ROS) production after cathachunine treatment was determined by 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining. Cell cycle arrest of the S phase was also observed in HL60 cells after cathachunine treatment. RESULTS: The WST-1 and LDH assays showed that Catharanthus alkaloids were cytotoxic toward human leukemia cells to a greater extent than toward normal human endothelial cells, and the anti-proliferation and pro-apoptosis abilities of cathachunine were much more potent than other previously reported alkaloids. The induction of apoptosis by cathachunine occurred through an ROS-dependent mitochondria-mediated intrinsic pathway rather than an extrinsic pathway, and was regulated by the Bcl-2 protein family. CONCLUSION: An unprecedented bisindole alkaloid cathachunine which lost C-18' and C-19' was isolated from C. roseus. It exerted a potent antitumor effect toward human leukemia cells through the induction of apoptosis via an intrinsic pathway. Thus, this study provides evidence for a new lead compound from a natural source for anti-cancer investigations.


Assuntos
Alcaloides/farmacologia , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Leucemia/tratamento farmacológico , Extratos Vegetais/farmacologia , Antineoplásicos/uso terapêutico , Catharanthus/química , China , Humanos , Células K562/efeitos dos fármacos
11.
Chem Biol Drug Des ; 88(2): 293-301, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-26992112

RESUMO

Tuberculosis (TB) is a highly contagious disease mainly caused by Mycobacterium tuberculosis H37 RV . Antitubercular (anti-TB) bioassay-guided isolation of the CHCl3 extract of the leaves and stems of the medicinal plant Ardisia gigantifolia led to the isolation of two anti-TB 5-alkylresorcinols, 5-(8Z-heptadecenyl) resorcinol (1) and 5-(8Z-pentadecenyl) resorcinol (2). We further synthesized 15 derivatives based on these two natural products. These compounds (natural and synthetic) were evaluated for their anti-TB activity against Mycobacterium tuberculosis H37 RV . Resorcinols 1 and 2 exhibited anti-TB activity with MIC values at 34.4 and 79.2 µm in MABA assay, respectively, and 91.7 and 168.3 µm in LORA assay, respectively. Among these derivatives, compound 8 was found to show improved anti-TB activity than its synthetic precursor (2) with MIC values at 42.0 µm in MABA assay and 100.2 µm in LORA assay. The active compounds should be regarded as new hits for further study as a novel class of anti-TB agents. The distinct structure-activity correlations of the parent compound were elucidated based on these derivatives.


Assuntos
Antituberculosos/química , Antituberculosos/isolamento & purificação , Ardisia/química , Bioensaio , Extratos Vegetais/farmacologia , Resorcinóis/química , Resorcinóis/isolamento & purificação , Antituberculosos/farmacologia , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Resorcinóis/farmacologia , Espectrometria de Massas por Ionização por Electrospray
12.
J Ethnopharmacol ; 179: 128-36, 2016 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-26190352

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Ligustrum purpurascens Y.C. Yang (Oleaceae) is traditionally recorded as "Ku Ding Cha", a kind of functional tea in southern China for about two thousand years, which has been reported with sore throat alleviating and pathogenic heat expelling effects. However, there are no scientific studies demonstrating its antiviral activity. THE AIM OF THE STUDY: This study is aimed at investigating the anti-influenza virus effects of phenylethanoid glycosides isolated from L. purpurascens (LPG) as well as its corresponding mechanisms. MATERIALS AND METHODS: In vitro, hemagglutination assay was employed to detect the influenza virus titer; In vivo, C57BL/6J mice were given oral administration of LPG (100mg/kg, 300mg/kg, 900mg/kg) or ribavirin (100mg/kg) once daily for 5 successive days. Meanwhile, on the second day, mice were infected intranasally (i.n.) with A/FM/1/47 H1N1 virus. Mice survival rate and other clinical index were monitored for 15 days. Infected mice were sacrificed to measure the lung lesion and stained with hematoxylin-eosin. Flow cytometry analyses spleen lymphocytes and interferon-γ (IFN-γ) level. The IFN-γ knockout mice (IFN-γ(-/-) mice, C57BL/6J) which had been verified lacking IFN-γ through Western Blot, were applied in the death-protection test to identify the role of IFN-γ played in LPG antiviral effect. RESULTS: In vitro, LPG at 0.5mg/ml inhibited Influenza A Virus H1N1 type (H1N1) infection of MDCK cells. In vivo, LPG at 300 and 900mg/kg significantly decreased the mouse lung index (p<0.05), alleviated influenza-induced lethality and clinical symptoms, and therefore enhanced mouse survival (p<0.05). More detailed experiments demonstrated that antiviral cytokine IFN-γ was involved in the antiviral effect of LPG. Flow cytometric analysis revealed that LPG (900mg/kg) significantly induced secretion of IFN-γ by splenic CD4(+) and CD8(+) cells (p<0.05). Moreover, LPG (900mg/kg) protected wild-type C57BL/6J mice from H1N1 injury, whereas LPG-mediated survival protection disappeared in IFN-γ(-/-) mice. CONCLUSION: These results suggest that up-regulating endogenous IFN-γ by LPG may represent a novel therapeutic approach for H1N1 infection.


Assuntos
Antivirais/farmacologia , Glicosídeos/farmacologia , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana/tratamento farmacológico , Indutores de Interferon/farmacologia , Interferon gama/biossíntese , Ligustrum/química , Animais , Antivirais/toxicidade , Citocinas/metabolismo , Cães , Feminino , Humanos , Influenza Humana/virologia , Interferon gama/genética , Ligustrum/toxicidade , Pulmão/virologia , Contagem de Linfócitos , Células Madin Darby de Rim Canino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Edema Pulmonar/tratamento farmacológico , Edema Pulmonar/patologia , Ribavirina/farmacologia , Ribavirina/uso terapêutico , Análise de Sobrevida
13.
Int J Mol Sci ; 16(11): 27978-87, 2015 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-26610490

RESUMO

Henrin A (1), a new ent-kaurane diterpene, was isolated from the leaves of Pteris henryi. The chemical structure was elucidated by analysis of the spectroscopic data including one-dimensional (1D) and two-dimensional (2D) NMR spectra, and was further confirmed by X-ray crystallographic analysis. The compound was evaluated for its biological activities against a panel of cancer cell lines, dental bacterial biofilm formation, and HIV. It displayed anti-HIV potential with an IC50 value of 9.1 µM (SI = 12.2).


Assuntos
Fármacos Anti-HIV/química , Fármacos Anti-HIV/farmacologia , Diterpenos de Caurano/química , Diterpenos de Caurano/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Pteris/química , Fármacos Anti-HIV/envenenamento , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Linhagem Celular , Diterpenos de Caurano/isolamento & purificação , HIV-1/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Modelos Moleculares , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Extratos Vegetais/isolamento & purificação
14.
Int J Dermatol ; 53(10): 1213-20, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25041445

RESUMO

OBJECTIVES: This study was designed to explore the pathogenesis of psoriasis and to identify potential bio-targets. Genome array technology was used to analyze the gene expression profiles of lesional and non-lesional psoriatic skin samples and normal skin samples. METHODS: Gene expression profile GSE14905 was downloaded from the Gene Expression Omnibus (GEO) database. This included skin biopsy samples from normal healthy donors (n = 21), lesional skin biopsy samples from psoriasis patients (n = 33), and non-lesional skin biopsy samples from psoriasis patients (n = 28). Differentially expressed genes (DEGs) were identified using the Limma package in R language. Functions of specific DEGs were predicted by Gene Ontology (GO) enrichment analysis. A protein-protein interaction network was constructed to display the interactions among common DEGs. Finally, DAVID and WebGestalt were used to achieve a functional analysis of common DEGs. RESULTS: Totals of 1020, 562, and 643 genes, respectively, were identified as being differentially expressed in normal versus lesional, normal versus non-lesional, and lesional versus non-lesional samples. The specific DEGs in the three groups were enriched for several GO terms, including mitotic cell cycle, immune response, and response to organic matter. The 40 common DEGs in the three groups may be involved in the defense response pathway in the development of psoriasis. Furthermore, three genes (RGS1, SOCS3, and NAMPT) may play key roles in distinguishing lesional and non-lesional tissues from normal tissues, and 10 genes (PTRRC, ALDH1A3, SAMSA1, C15orf48, ZC3H12A, SOD2, IL8, LTF, RHCG, and IL7R) may play key roles in distinguishing non-lesional from normal and lesional samples. CONCLUSIONS: These genes may be considered as potential diagnostic markers and targets of therapeutics in psoriasis.


Assuntos
Perfilação da Expressão Gênica , Expressão Gênica , Análise de Sequência com Séries de Oligonucleotídeos , Psoríase/genética , Humanos
15.
J Agric Food Chem ; 61(26): 6568-73, 2013 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-23745668

RESUMO

Panax ginseng C. A. Mey has been used as a traditional medicine and functional food in Asia for thousands of years for its improvement of human immunity and metabolism and its antitumor and antifatigue activities. This study reports the impact of storage conditions and storage period on the quality of P. ginseng. The contents of four major ginsenosides in P. ginseng and phosphorylation activities of Akt of ginseng extracts were affected by both storage conditions and storage period. In contrast, the ATP generation capacity of ginseng extracts was affected by storage conditions, but not by storage period. The results showed that the quality of P. ginseng could be well maintained at a relative humidity between 70% and 90%, and dry conditions might decrease the quality of P. ginseng. Through dual-index evaluation, the present study extended our knowledge on the changes of ginsenosides and bioactivities in P. ginseng with respect to different storage conditions and storage periods.


Assuntos
Qualidade dos Alimentos , Armazenamento de Alimentos , Ginsenosídeos/análise , Panax/química , Animais , Antineoplásicos Fitogênicos/análise , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular , Células Cultivadas , China , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Ginsenosídeos/farmacologia , Humanos , Panax/crescimento & desenvolvimento , Fosforilação/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Processamento de Proteína Pós-Traducional/efeitos dos fármacos , Ratos , Transdução de Sinais/efeitos dos fármacos
16.
J Ethnopharmacol ; 144(3): 584-91, 2012 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-23064286

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Ligustrum purpurascens, named as "Ku ding cha", has been used as a kind of functional tea in southern China for about two thousand years, which has the effects on diuresis, anti-hypertension, weight-loss and anti-inflammation. THE AIM OF THE STUDY: This study was aimed to investigate the immune enhancement effects of the crude phenylethanoid glycosides (CPGs) from Ligustrum. Purpurascens on mice and analyze the chemical profiles of phenylethanoid glycosides in the CPGs. MATERIALS AND METHODS: The immune functions enhancing potential of CPGs was determined using serum hemolysin antibody, phagocytosis, splenocyte antibody production, and NK cells activity assays. The contents of five major constituents in the crude glycosides of Ligustrum purpurascens were determined by using liquid chromatography, other five glycosides were deduced according to their UV and MS spectra compared with the literature as well. RESULTS: In the immunizing experiment, mice treated with different doses of CPGs showed an increase (p<0.01) in the haemagglutination titre compared with the control group. The increases (p<0.05) were found to be significant at doses of 440 mg/kg and 1.32 g/kg in the experiments of antibody production of spleen cells, MΦ phagocytosis of chicken RBCs and NK cell activity. Further chemical characterization yielded 10 constituents from CPGs, five glycosides were quantified by HPLC and the structures of other five compounds were speculated according to their UV and MS spectra. CONCLUSION: The results suggested that phenylethanoid glycosides from Ligustrum purpurascens have immunomodulatory effects on mice.


Assuntos
Glicosídeos/farmacologia , Fatores Imunológicos/farmacologia , Ligustrum , Extratos Vegetais/farmacologia , Animais , Anticorpos/imunologia , Linhagem Celular Tumoral , Galinhas , Eritrócitos/imunologia , Feminino , Glicosídeos/isolamento & purificação , Testes de Hemaglutinação , Técnica de Placa Hemolítica , Fatores Imunológicos/isolamento & purificação , Células Matadoras Naturais/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/imunologia , Camundongos , Panax , Fagocitose/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Folhas de Planta , Raízes de Plantas , Ovinos , Baço/citologia , Baço/efeitos dos fármacos , Baço/imunologia
17.
Zhong Xi Yi Jie He Xue Bao ; 6(12): 1246-9, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19063837

RESUMO

OBJECTIVE: To explore the clinical efficacy of Herba Saxifragae cream (HS), a compound of traditional Chinese herbal medicine, on chronic eczema. METHODS: A total of 42 cases of chronic eczema were randomly divided into HS group (22 cases) and hydrocortisone (HC) group (20 cases). To the HS group, HS was externally applied twice a day continuously for 4 weeks. To the HC group, hydrocortisone was externally applied twice a day continuously for 4 weeks. The total score of symptom score reducing index (SSRI) was calculated before and after treatment in terms of itching degree, lesion shape and lesion area, in the evaluation of the clinical efficacy of HS on chronic eczema. RESULTS: After 2-week treatment, the total response rate of HS group was 77.3%, and the total response rate of HC group was 70.0%. After 4-week treatment, the total response rates were 86.4% and 85.0% in HS and HC groups respectively. There was no statistical difference in total scores of SSRI between the two groups (P>0.05), and neither was there the difference in the score of itching degree, lesion shape and lesion area (P>0.05). CONCLUSION: Herba Saxifragae cream has the same effect as hydrocortisone in treating chronic eczema.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Eczema/tratamento farmacológico , Fitoterapia , Saxifragaceae/química , Adulto , Idoso , Doença Crônica , Feminino , Humanos , Hidrocortisona/uso terapêutico , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento
18.
Zhong Xi Yi Jie He Xue Bao ; 6(6): 586-90, 2008 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-18559235

RESUMO

OBJECTIVE: To explore the clinical efficacy of Qinzhu Liangxue Decoction (QZLXD), a compound traditional Chinese herbal medicine, in patients with blood-heat type psoriasis vulgaris. METHODS: Fifty-eight patients diagnosed with blood-heat type psoriasis vulgaris were randomly divided into two groups: QZLXD group (30 cases) and ampeptide group (28 cases). Patients in both groups were treated for 4 weeks. Psoriasis Area and Severity Index (PASI) score, Dermatology Life Quality Index (DLQI) score and the level of serum vascular endothelial growth factor (VEGF) were obtained to evaluate the efficacy of the two treatments. RESULTS: There was a better curative result in QZLXD group than in ampeptide group (P<0.05). The response rates in QZLXD and ampeptide groups were 83.33% and 64.28% respectively. DLQI score was also significantly improved during 4-week treatment in QZLXD and ampeptide groups (P<0.05). There was no significant difference in VEGF level between QZLXD and ampeptide groups. CONCLUSION: QZLXD is remarkably advantageous in treatment of psoriasis vulgaris of blood-heat syndrome and improvement of patient's quality of life. The mechanism may be related to decrease in serum VEGF level and action against neogenesis of blood vessels.


Assuntos
Diagnóstico Diferencial , Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Tradicional Chinesa , Fitoterapia , Psoríase/tratamento farmacológico , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fator A de Crescimento do Endotélio Vascular/sangue
19.
Toxicol Sci ; 78(2): 241-7, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-14976354

RESUMO

The effect of the organophosphorous insecticide paraoxon on the integrity of the blood-brain barrier (BBB) and permeability of pyridostigmine (PYR), a peripheral inhibitor of cholinesterase activity, was examined in Long Evans rats. The integrity of the BBB was examined by measuring the number of capillaries leaking horseradish peroxidase, which was injected into the heart. Treatment with paraoxon at 100 microg/kg, intramuscularly, resulted in a 3- to 4-fold increase in the number of leaky capillaries in young rats (25 to 30 days old) but not in older rats (90 days old). Interestingly, young rats treated with PYR (30 mg/kg, po) 50 min before treatment with paraoxon showed an inhibited effect of paraoxon on the BBB. Furthermore, no increase in the degree of inhibition of acetylcholinesterase activity was observed in young rats treated with PYR before paraoxon compared with young rats treated with paraoxon alone. Cholinergic toxicity, as assessed by changes in behavior, was not observed in young rats treated with paraoxon alone; but, slight signs of cholinergic toxicity were observed in rats treated with PYR. Young rats treated with both PYR and paraoxon did not exhibit more extensive signs of toxicity than rats treated with paraoxon alone or PYR alone. The results indicate that treatment with paraoxon can compromise BBB permeability at dosages that do not induce cholinergic toxicity, but only in young rats. Also, PYR pre-exposure appears to protect the BBB from the paraoxon-induced alterations.


Assuntos
Comportamento Animal/efeitos dos fármacos , Barreira Hematoencefálica/efeitos dos fármacos , Inibidores da Colinesterase/farmacologia , Inseticidas/toxicidade , Paraoxon/toxicidade , Brometo de Piridostigmina/farmacologia , Fatores Etários , Animais , Barreira Hematoencefálica/metabolismo , Encéfalo/enzimologia , Encéfalo/metabolismo , Capilares/efeitos dos fármacos , Capilares/enzimologia , Capilares/fisiopatologia , Inibidores da Colinesterase/metabolismo , Colinesterases/análise , Colinesterases/metabolismo , Interações Medicamentosas , Peroxidase do Rábano Silvestre/administração & dosagem , Peroxidase do Rábano Silvestre/análise , Humanos , Masculino , Paraoxon/antagonistas & inibidores , Síndrome do Golfo Pérsico/etiologia , Síndrome do Golfo Pérsico/metabolismo , Brometo de Piridostigmina/metabolismo , Ratos , Ratos Long-Evans
20.
Toxicol Sci ; 69(1): 157-64, 2002 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12215670

RESUMO

Pyridostigmine, a carbamate cholinesterase (ChE) inhibitor, has been used for decades in the treatment of the autoimmune disorder myasthenia gravis and was used prophylactically to protect soldiers from possible organophosphorus nerve agent exposures during the Persian Gulf War. Pyridostigmine is a charged, quaternary compound and thus would not be expected to easily pass the blood-brain barrier. Some studies have suggested, however, that stress may alter blood-brain barrier integrity and allow pyridostigmine to enter the brain. We evaluated the effects of acute and repeated restraint stress on functional signs of cholinergic toxicity (i.e., autonomic dysfunction and involuntary movements) and brain regional cholinesterase inhibition following either acute or repeated pyridostigmine exposures. The acute, oral maximum-tolerated dosage (MTD) of pyridostigmine was estimated at 30 mg/kg. Peak ChE inhibition in whole blood occurred from 0.5 to 4 h after MTD exposure, whereas minimal (<20%) brain ChE inhibition was noted. For acute restraint studies, rats were either (1) restrained for 90 min and then given pyridostigmine (30 mg/kg, po), (2) given pyridostigmine and immediately restrained for 60 min, or (3) restrained for 3 h, given pyridostigmine, and restrained for an additional 60 min. In all cases, rats were evaluated for cholinergic toxicity (SLUD signs and involuntary movements) and sacrificed 1 h after pyridostigmine treatment. Plasma corticosterone was significantly elevated immediately after a single 60-min session of acute restraint stress, but returned to control levels by 1 and 3 h later. Pyridostigmine-induced toxicity was not enhanced nor was brain ChE inhibition altered by acute restraint stress. Blood-brain barrier permeability, assessed by accumulation of horseradish peroxidase in brain regions following intracardiac injection, was not increased by restraint stress. For repeated restraint studies, rats were given pyridostigmine (0, 3, or 10 mg/kg/day) immediately prior to daily restraint (60 min) for 14 consecutive days. Plasma corticosterone was elevated at 1 and 7 days but not at 14 days. Pyridostigmine-treated rats in both dosage groups exhibited slight signs of toxicity for the first 3-5 days, after which cholinergic signs dissipated. Repeated restraint had little effect on functional signs of pyridostigmine toxicity, however. Whole blood and diaphragm ChE were markedly reduced 1 h after the last treatment, but stress had no influence on ChE inhibition in either peripheral or central tissues. The results suggest that acute and repeated restraint stress have little effect on pyridostigmine neurotoxicity or apparent entry of pyridostigmine into the brain.


Assuntos
Encéfalo/enzimologia , Inibidores da Colinesterase/toxicidade , Síndromes Neurotóxicas/enzimologia , Síndromes Neurotóxicas/patologia , Brometo de Piridostigmina/toxicidade , Estresse Psicológico/patologia , Doença Aguda , Animais , Barreira Hematoencefálica/fisiologia , Encéfalo/efeitos dos fármacos , Colinesterases/metabolismo , Doença Crônica , Corticosterona/sangue , Peroxidase do Rábano Silvestre , Masculino , Sistema Nervoso Periférico/enzimologia , Sistema Nervoso Periférico/patologia , Ratos , Ratos Sprague-Dawley , Restrição Física
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA