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1.
J Environ Manage ; 325(Pt A): 116502, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36274310

RESUMO

Although the past twenty years have witnessed China's remarkable economic development, the cost in terms of greenhouse gas emissions and a deteriorating environment has been enormous. Numerous studies have revealed the influence of household factors on household carbon dioxide emissions (HCEs) and called for a reduction of HCEs to mitigate climate change, but few have focused on assessing the most significant household driving factors of HCEs. Using statistical data between 2005 and 2019 in Jiangsu, China, this study developed an extended stochastic impact by regression on population, affluence, and technology (STIRPAT) model to assess the most significant driving factors of HCEs. The results show that the most significant driving factors are household size, total population, unemployment, and urbanisation rate. The study found that HCEs are positively impacted by household size while negatively impacted by the unemployment rate. Based on the study's findings, the following suggestions are proposed to lower HCEs: (i) establish an optimal consumption concept to guide residents towards consuming reasonably; (ii) cultivate a low-carbon concept among residents and promote low-carbon emissions living; and (iii) pay close attention to population structure factors and formulate effective measures accordingly. The study provides insightful information on the key driving factors of HCEs, which can facilitate achieving carbon emissions neutrality.


Assuntos
Dióxido de Carbono , Gases de Efeito Estufa , Dióxido de Carbono/análise , Desenvolvimento Econômico , China , Gases de Efeito Estufa/análise , Tecnologia
2.
Behav Brain Res ; 438: 114211, 2023 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-36368442

RESUMO

Major depressive disorder is a complex psychiatric disorder with a high prevalence rate worldwide. Previous studies have demonstrated the involvement of the prelimbic cortex (PL) in mediating depressive-like behavior, however, the exact molecular mechanism taking place in the PL remains unclear. In the present study, we conducted high-throughput sequencing of mRNAs and miRNAs in PL tissue harvested from chronic social defeat stress (CSDS) susceptible male mice. We identified 59 differentially expressed mRNAs and 6 differentially expressed miRNAs, in which 40 mRNAs and 3 miRNAs were up-regulated, while 19 mRNAs and 3 miRNAs were down-regulated. Integrated analysis of miRNA-mRNA networks suggested that GPR35 signaling might be involved in CSDS-induced depressive-like behaviors. RT-PCR and western blot assays validated that Abra, Sell and GPR35 were up-regulated. Functionally, inhibition of GPR35 in the PL ameliorated CSDS-induced depressive-like behaviors. Thus, the present study provided a global view of mRNA and miRNA profiles in the PL of male stress susceptible mice, and suggested that GPR35 signaling was associated with CSDS-induced depressive-like behaviors. These results may be valuable for further investigations of the molecular regulatory mechanisms in stress-induced depression.


Assuntos
Transtorno Depressivo Maior , MicroRNAs , Camundongos , Masculino , Animais , Derrota Social , Depressão/metabolismo , Estresse Psicológico/metabolismo , RNA Mensageiro , MicroRNAs/genética , Suscetibilidade a Doenças , Camundongos Endogâmicos C57BL , Receptores Acoplados a Proteínas G
3.
J Environ Manage ; 325(Pt B): 116620, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36323123

RESUMO

The structure of biochar produced at various pyrolysis temperatures influences metal geochemical behavior. Here, the impact of wheat straw-derived biochar (300, 500, and 700 °C) on the immobilization and transformation of metals in the contaminated soil-plant system was assessed. The findings of the sequential extraction revealed that biochar additives had a substantial influence on the speciation of Cr, Ni, Pb, and Zn in the contaminated soil. The lowest F1 (exchangeable and soluble fraction) + F2 (carbonate fraction) accounted for Cr (44%) in WB-300, Ni (43.87%) in WB-500, Pb (43.79%), and Zn (49.78%) in WB-700 with applied amendments of their total amounts. The characterization results indicated that high pyrolysis temperatures (300-700 °C) increased the carbon-containing groups with the potential to adsorb metals from the soil-plant environment. The bioconcentration and translocation factors (BCF and TF) were less than 1, indicating that metal concentration was restricted to maize roots and translocation to shoots. Reactive oxygen species (ROS) intracellularly influence metal interactions with plants. Electron paramagnetic resonance (EPR) was performed to determine hydroxyl radical generation (•OH) in plant segments to assess the dominance of free radicals (FRs). Consequently, the formation of •OH significantly depends on the pyrolysis temperature and the interaction with a contaminated soil-plant environment. Thus, metal transformation can be effectively decreased in the soil-plant environment by applying WB amendments.


Assuntos
Metais Pesados , Poluentes do Solo , Poluentes do Solo/análise , Chumbo , Carvão Vegetal/química , Solo/química , Metais Pesados/química , Radicais Livres
4.
J Colloid Interface Sci ; 630(Pt B): 436-443, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36334480

RESUMO

Here, we report a facile approach to fabricate large area ordered arrays of TiO2 hierarchical nanostructures through space-confined seeding and growth on inverted pyramid templates. The mechanisms of space-confined seeding and growth have been systematically explored and studied. The drying TiO2 seed precursor solution prefers to accumulate on the narrow structures including the centre and edges of the inverted pyramid structures, which facilitates to reduce the free energy of the precursor solution surface and form crystal seeds. Followed by hydrothermal treatment, selective growth of TiO2 hierarchical nanostructures on desirable locations, such as only on the centre, only on the edges, or on the entire surface of the inverted pyramid templates, can be achieved. In addition, the growth temperature, duration and solvents affect the morphology of TiO2 hierarchical nanostructures. This work may provide a universal approach to obtain ordered arrays of metal oxide (e.g. ZnO and MnO2, etc.) nanostructures for applications in optics, electrics, energy, and catalysis.

5.
EJHaem ; 3(4): 1154-1164, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36467814

RESUMO

In this post hoc subgroup analysis of 200 patients enrolled in China from the phase III PHOENIX trial (N = 838, NCT01855750), addition of ibrutinib to rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP) did not improve event-free survival (EFS) versus placebo+R-CHOP in the intent-to-treat (ITT; n = 200, hazard ratio [HR] = 0.83, 95% confidence interval [CI]: 0·509-1.349; p = 0.4495) or activated B-cell-like (ABC; n = 141 [based on available gene-expression profiling data], HR = 0.86, 95% CI: 0.467-1.570; p = 0.6160) subpopulations. However, ibrutinib+R-CHOP improved EFS (HR = 0·50, 95% CI: 0.251-1.003) and progression-free survival (PFS; HR = 0.48, 95% CI: 0.228-1.009) versus placebo+R-CHOP in patients aged <60 but not ≥60 years. Grade ≥3 serious treatment-emergent adverse events occurred more with ibrutinib+R-CHOP (45·6% vs. 31·3%). The percentage of patients receiving ≥6 cycles of R-CHOP was similar across treatment arms in those <60 years. A numerical trend was seen towards improved EFS and PFS with ibrutinib+R-CHOP versus placebo+R-CHOP in patients with MYC-high/BCL2-high co-expression. In this slightly younger Chinese subgroup, ibrutinib+R-CHOP did not improve EFS in the ITT and ABC subpopulations but improved outcomes with manageable safety in patients <60 years, consistent with overall PHOENIX study outcomes.

6.
Comput Biol Med ; 152: 106344, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36470142

RESUMO

In recent years, emotion recognition based on electroencephalography (EEG) signals has attracted plenty of attention. Most of the existing works focused on normal or depressed people. Due to the lack of hearing ability, it is difficult for hearing-impaired people to express their emotions through language in their social activities. In this work, we collected the EEG signals of hearing-impaired subjects when they were watching six kinds of emotional video clips (happiness, inspiration, neutral, anger, fear, and sadness) for emotion recognition. The biharmonic spline interpolation method was utilized to convert the traditional frequency domain features, Differential Entropy (DE), Power Spectral Density (PSD), and Wavelet Entropy (WE) into the spatial domain. The patch embedding (PE) method was used to segment the feature map into the same patch to obtain the differences in the distribution of emotional information among brain regions. For feature classification, a compact residual network with Depthwise convolution (DC) and Pointwise convolution (PC) is proposed to separate spatial and channel mixing dimensions to better extract information between channels. Dependent subject experiments based on 70% training sets and 30% testing sets were performed. The results showed that the average classification accuracies by PE (DE), PE (PSD), and PE (WE) were 91.75%, 85.53%, and 75.68%, respectively which were improved by 11.77%, 23.54%, and 16.61% compared with DE, PSD, and WE. Moreover, the comparison experiments were carried out on the SEED and DEAP datasets with PE (DE), which achieved average accuracies of 90.04% (positive, neutral, and negative) and 88.75% (high valence and low valence). By exploring the emotional brain regions, we found that the frontal, parietal, and temporal lobes of hearing-impaired people were associated with emotional activity compared to normal people whose main emotional brain area was the frontal lobe.

7.
Artigo em Inglês | MEDLINE | ID: mdl-36455076

RESUMO

Emotion analysis has been employed in many fields such as human-computer interaction, rehabilitation, and neuroscience. But most emotion analysis methods mainly focus on healthy controls or depression patients. This paper aims to classify the emotional expressions in individuals with hearing impairment based on EEG signals and facial expressions. Two kinds of signals were collected simultaneously when the subjects watched affective video clips, and we labeled the video clips with discrete emotional states (fear, happiness, calmness, and sadness). We extracted the differential entropy (DE) features based on EEG signals and converted DE features into EEG topographic maps (ETM). Next, the ETM and facial expressions were fused by the multichannel fusion method. Finally, a deep learning classifier CBAM_ResNet34 combined Residual Network (ResNet) and Convolutional Block Attention Module (CBAM) was used for subject-dependent emotion classification. The results show that the average classification accuracy of four emotions recognition after multimodal fusion achieves 78.32%, which is higher than 67.90% for facial expressions and 69.43% for EEG signals. Moreover, visualization by the Gradient-weighted Class Activation Mapping (Grad-CAM) of ETM showed that the prefrontal, temporal and occipital lobes were the brain regions closely related to emotional changes in individuals with hearing impairment.

8.
Food Chem Toxicol ; : 113532, 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36455683

RESUMO

The development of breast cancer is usually related to multiple pathways. A combinatory therapeutic system that combines drug/siRNA targeting several independent pathways has become an attractive approach to reduce the side effects and improve the efficiency of antitumor drugs. Herein, we designed a unique micelle-liposome hybrid nanoparticle platform for the combinatory administration of the cytotoxic drug DOX and Sphk2-siRNA for the treatment of multidrug-resistant (MDR) cancer. The synthesized lipid dioleoyl ethanolamine (DE) and pH-responsive DOPE were used to produce DOX/siRNA co-loaded hybrid nanoparticle (DOX-MC-siSphk2/ASLNP), with high drug-loading capacity and transfection efficacy. We demonstrated that simultaneous cellular endocytosis of DOX/siRNA induced by nanoparticles in MCF-7/ADR cells could acquire higher drug cytotoxicity and contribute to increasing the apoptosis of tumor cell. Furthermore, DOX-MC-siSphk2/ASLNP could significantly block the tumor growth of MDR breast cancer in xenograft mouse model with lower cardiotoxicity.

9.
Redox Biol ; 58: 102522, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36335763

RESUMO

The balance between oxidation and antioxidant is crucial for maintaining homeostasis. Once disrupted, it can lead to various pathological outcomes and diseases, such as depression. Oxidative stress can result in or aggravate a battery of pathological processes including mitochondrial dysfunction, neuroinflammation, autophagical disorder and ferroptosis, which have been found to be involved in the development of depression. Inhibition of oxidative stress and related pathological processes can help improve depression. In this regard, the nuclear factor erythroid 2-related factor 2 (Nrf2) in the antioxidant defense system may play a pivotal role. Nrf2 activation can not only regulate the expression of a series of antioxidant genes that reduce oxidative stress and its damages, but also directly regulate the genes related to the above pathological processes to combat the corresponding alterations. Therefore, targeting Nrf2 has great potential for the treatment of depression. Activation of Nrf2 has antidepressant effect, but the specific mechanism remains to be elucidated. This article reviews the key role of Nrf2 in depression, focusing on the possible mechanisms of Nrf2 regulating oxidative stress and related pathological processes in depression treatment. Meanwhile, we summarize some natural and synthetic compounds targeting Nrf2 in depression therapy. All the above may provide new insights into targeting Nrf2 for the treatment of depression and provide a broad basis for clinical transformation.

10.
J Neurol ; 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36385201

RESUMO

OBJECTIVE: To describe the disease progression of Duchenne muscular dystrophy (DMD) in the pelvic and thigh muscles over 1-year using multiple-parameter quantitative magnetic resonance imaging (qMRI), and to determine the most responsive muscle and predict subclinical disease progression in functionally stable patients. METHODS: Fifty-four DMD patients (mean age 8.9 ± 2.5, range 5-15 years) completed baseline and 1-year follow-up qMRI examinations/biomarkers [3-point Dixon/fat fraction (FF); T1 mapping/T1; T2 mapping/T2]. Meanwhile, clinical assessments [NorthStar ambulatory assessment (NSAA) score] and timed function tests were performed in DMD patients. Twenty-four healthy male controls (range 5-15 years) accomplished baseline qMRI examinations. Group differences were compared using the Wilcoxon test. The standardized response mean (SRM) was taken as the responsiveness to the disease progression index. RESULTS: FF, T1, and T2 in all DMD age subgroups changed significantly over 1-year (P < 0.05). Even in functionally stable patients (NSAA score increased, unchanged, or decreased by 1-point) over 1-year, significant increases in FF and T2 and decreases in T1 were observed in gluteus maximus (GMa), gluteus medius, vastus lateralis, and adductor magnus (P < 0.05). Overall, the SRM of FF, T1, and T2 was all the highest in GMa, which were 1.25, - 0.92, and 0.93, respectively. CONCLUSIONS: qMRI biomarkers are responsive to disease progression and can also detect subclinical disease progression in functionally stable DMD patients over 1-year. GMa is the most responsive to disease progression of all the muscles analyzed. TRIAL REGISTRATION: Chinese Clinical Trial Registry ( http://www.chictr.org.cn/index.aspx ) ChiCTR1800018340, 09/12/2018, prospectively registered.

11.
Clin Exp Rheumatol ; 2022 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-36377573

RESUMO

OBJECTIVES: Many studies have found that CD38 expression is increased in rheumatoid arthritis (RA), an autoimmune disease in which immune tolerance is dysregulated. Inhibition of CD38 expression or activity can significantly alleviate collagen-induced arthritis (CIA), a well-known animal model used for the study of RA. This study aimed to confirm the therapeutic effect of 78c, a specific inhibitor of CD38, and the role of CD38+ NK cells in immune imbalance in RA. METHODS: CIA mice were injected with 78c to observe the therapeutic effect. CD38+ NK cells were extracted from human peripheral blood and treated with 78c. The pretreated NK cells were co-cultured with CD4+ T cells. RESULTS: We found that 78c significantly suppressed joint inflammation; reduced the levels of B cells, IL-6 and TNF-α; and increased the levels of IL-10, energy metabolism and spontaneous movement in CIA mice. 78c treatment also increased Treg cell numbers and decreased the Th1/Th2 ratio in the CIA model animals. Moreover, the proportion of CD38+ NK cells was increased in the CIA mice and significantly decreased following 78c treatment. Human CD4+ T cells that were co-cultured with 78c-pretreated CD38+ NK cells differentiated into more Treg cells and had lower Th17/Treg and Th1/Th2 ratios than CD4+ T cells co-cultured with CD38+ NK cells without the pretreatment. Transcriptomic analyses demonstrated that 78c changed expression pro les in CD38+ NK cells. CONCLUSIONS: These results suggested that 78c could be used for the treatment of RA and CIA as it alleviates the inhibitory effect of CD38+ NK cells on CD4+ T cell differentiation to Treg cells to restore immune balance.

12.
Front Surg ; 9: 984857, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36439528

RESUMO

Objective: Conventional second window indocyanine green (SWIG) technique has been widely attempted in near-infrared fluorescence (NIRF) imaging for intraoperative navigation of tumor radical resection. Nevertheless, the overuse of indocyanine green (ICG) led to an increased risk of drug lethal allergy and high medical cost. This prospective study was to explore clinical application of modified low-dose SWIG technique in guiding dermatofibrosarcoma protuberans (DFSPs) radical resection. Method: Patients with DFSPs were randomly assigned to control and experimental group. The ICG was injected intravenously 24 h before surgery, at a dose of 3.5 mg/kg in the control group and 25 mg/patient in the experiment group, respectively. Intraoperative NIRF imaging included serial views of gross tumor, tumor bed and cross-sectional specimen. Results: Although NIRF imaging of gross tumor and tumor bed in the experimental group demonstrated similar sensitivity and negative predictive value, the specificity and positive predictive value were obviously higher compared to control group. The tumor-to-background ratios of cross-sectional specimens in the experimental group was significantly higher than in the control group (P = 0.000). Data in both groups displayed that there was a positive correlation of tumor size in cross-sections between integrated histopathologic photomicrographs and NIRF imaging of specimen views (P = 0.000). NIRF imaging of cross-sectional specimens had a significant decrease in time cost, and an increase in the ability of examining more surgical margins (P = 0.000). Conclusion: This is the first study to demonstrate that a low-dose SWIG technique could improve the accuracy of near-infrared fluorescence image-guided dermatofibrosarcoma protuberans resection.Clinical Trial Registration: ChiCTR2100050174; date of registration: August 18, 2021 followed by "retrospectively registered".

13.
Mol Pain ; 18: 17448069221144246, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36424837

RESUMO

Osteoarthritis (OA) is a common osteoarthropathy with chronic inflammatory pain as the core symptom in middle-aged and elderly people. LncRNA MEG3 (Maternally expressed gene 3) is involved in the development of OA via regulation of angiogenesis, which causes the activation and overexpression of transient receptor potential vanilloid type-1 (TRPV1). In this study, we investigated the mechanism of MEG3-TRPV1 signaling in chronic inflammatory pain (CIP) of rat model. Chronic inflammatory pain was modeled using subcutaneous microinjection of complete Freund's adjuvant (CFA) into the left hind paw of rats. We showed that TRPV1 mRNA and protein were significantly increased, while MEG3 mRNA was significantly decreased, in the DRG and SDH of CFA-induced rats. In addition, intrathecal injection of MEG3-overexpressing lentivirus significantly downregulated TRPV1 expression and alleviated chronic inflammatory pain in CFA-induced rats. Treatment with a TRPV1 antagonist also significantly relieved chronic inflammatory pain in CFA-induced rats. In general, our results reveal that MEG3 alleviates chronic inflammatory pain by downregulating TRPV1 expression. These findings may provide new therapeutic targets in the treatment of patients with OA.

14.
Zhongguo Zhen Jiu ; 42(11): 1317-20, 2022 Nov 12.
Artigo em Chinês | MEDLINE | ID: mdl-36397233

RESUMO

Physicians in the past dynasties have improved the theory of fire needling from the aspects of fire needling instruments, clinical efficacy, application scope, operation, precautions, etc., which promoted the clinical application of fire needling. Modern fire needling breaks through the traditional clinical taboos such as heat syndrome, face, forbidden acupoints, and no needle retention. By using modern fire needling with various types, characteristics and functions, multiple needles and multiple methods are used to treat various diseases, which can further exert the therapeutic effect of fire needling and promote the popularization and application of fire needle therapy.


Assuntos
Terapia por Acupuntura , Pontos de Acupuntura , Procedimentos Cirúrgicos Vasculares , Agulhas , Resultado do Tratamento
15.
Mater Today Bio ; 17: 100468, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36340592

RESUMO

Uncontrolled bleeding remains as a leading cause of death in surgical, traumatic, and emergency situations. Management of the hemorrhage and development of hemostatic materials are paramount for patient survival. Owing to their inherent biocompatibility, biodegradability and bioactivity, biopolymers such as polysaccharides and polypeptides have been extensively researched and become a focus for the development of next-generation hemostatic materials. The construction of novel hemostatic materials requires in-depth understanding of the physiological hemostatic process, fundamental hemostatic mechanisms, and the effects of material chemistry/physics. Herein, we have recapitulated the common hemostatic strategies and development status of biopolymer-based hemostatic materials. Furthermore, the hemostatic mechanisms of various molecular structures (components and chemical modifications) are summarized from a microscopic perspective, and the design based on them are introduced. From a macroscopic perspective, the design of various forms of hemostatic materials, e.g., powder, sponge, hydrogel and gauze, is summarized and compared, which may provide an enlightenment for the optimization of hemostat design. It has also highlighted current challenges to the development of biopolymer-based hemostatic materials and proposed future directions in chemistry design, advanced form and clinical application.

16.
Food Sci Nutr ; 10(11): 3876-3889, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36348812

RESUMO

In recent years, biologically active ingredients derived from natural plants or functional foods have raised considerable interests for its anti-obesity effect. Quinoa (Chenopodium quinoa Willd.) is a traditional staple food in the Andean regions of Peru which contains a variety of bioactive components. This study aimed to investigate the potential therapeutic effect of total saponins extracted from quinoa bran (TSQ) on obese rats and explore whether the underlying mechanism is related to intestinal microbiota. Results showed that TSQ could decrease the body weight gain and visceral fat accumulation in the obese rats. Moreover, trends in ameliorating insulin resistance and improved glucose tolerance were observed. Indeed, Pearson's correlations analysis revealed that the variation in gut microbial composition was highly correlated to insulin resistance, IL-6, and LPS levels. Collectively, these results suggest that the prevention of obesity and inflammation by TSQ may be mediated by the modulation of gut microbial composition.

17.
Infect Drug Resist ; 15: 6423-6430, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36349214

RESUMO

This is the first report describing co-infection of Scedosporium apiospermum and Lichtheimia corymbifera caused by biogas inhalation in two people without underlying medical conditions. Two patients fell into the same pig manure pit at the same time while rescuing another patient (this person died in a few hours) and inhaled biogas. Both patients were diagnosed with pulmonary fungal disease and developed acute liver failure around Day 52. Their results were negative for the 1,3-ß-d-glucan test and weakly positive for the galactomannan test. They were treated with amphotericin B and/or posaconazole without surgery. The patient in case 2 required amphotericin B deoxycholate aerosol inhalation to complete the treatment. Both patients recovered completely. For patients with mucormycosis confined to the lungs who cannot tolerate intravenous drip amphotericin B, increasing the dose of nebulised administration maybe a salvage regimen.

18.
Front Oncol ; 12: 871786, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36330486

RESUMO

Background: Immunotherapy plays an important role in the treatment of triple-negative breast cancer (TNBC). This study aimed to identify immune-related genes that are associated with the prognosis of patients with TNBC as possible targets of immunotherapy, alongside their related tumor-infiltrating lymphocytes (TILs). Methods: The clinical data and gene expression profiles of patients with breast cancer were extracted from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and divided into training (n = 1,053) and verification (n = 508) groups. CIBERSORT was used to predict the differences in immune cell infiltration in patient subsets that were stratified according to risk. Gene Ontology (GO) enrichment analysis was used to identify pathways associated with immune-related genes in patient subsets that were stratified according to risk. The clinical data and insulin-like growth factor 2 receptor (IGF2R) expression profiles of patients with breast cancer were extracted from METABRIC. The expression of IGF2R and TILs were evaluated in a cohort containing 282 untreated patients with TNBC. The correlations of IGF2R expression, TILs, and clinicopathological parameters with patient prognosis were analyzed in the whole cohort. Results: The prognostic model, which was composed of 26 immune-related gene pairs, significantly distinguished between high- and low-risk patients. Univariate and multivariate analyses indicated that the model was an independent prognostic factor for breast cancer. Among the identified genes, the expression of IGF2R significantly distinguished between high- and low-risk patients in TCGA (P = 0.008) and in METABRIC patients (P < 0.001). The expression of IGF2R was significantly associated with clinical risk factors such as TNBC, estrogen receptor (ER)-negative expression, human epidermal growth factor receptor 2 (HER2)-positive expression, and age ≤60 years old in METABRIC patients. In addition, the patients with IGF2R-positive expression had lower disease-free survival (DFS) rates than those with IGF2R-negative expression in the TNBC cohort (67.8% vs. 78.5%, P = 0.023). IGF2R expression also was significantly negatively correlated with TILs, particularly with CD8+ TILs and CD19+ TILs in the cohort of patients with TNBC. Conclusion: IGF2R can be used as an indicator of a poor prognosis in patients with TNBC and as a potential target and research direction for TNBC immunotherapy in the future.

19.
J Immunol ; 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36368721

RESUMO

Abnormally high follicle-stimulating hormone (FSH) has been reported to associate with cardiovascular diseases in prostate cancer patients with specific androgen deprivation therapy and in menopausal women. All of the cardiovascular diseases were involved in atherosclerosis. However, the pathogenic mechanism of FSH-associated atherosclerosis remains uncertain. Apolipoprotein E-deficient mice were chosen to develop atherosclerosis, of which the plaques were analyzed with administration of short- and long-term FSH imitating androgen deprivation therapy-induced and menopausal FSH elevation. The study showed that short- and long-term exposure of FSH significantly accelerated atherosclerosis progression in apolipoprotein E-deficient mice, manifested as strikingly increased plaques in the aorta and its roots, increased macrophage content, reduced fibrin, and an enlarged necrotic core, suggesting a decrease in plaque stability. Furthermore, expression profiles from the Gene Expression Omnibus GSE21545 dataset revealed that macrophage inflammation was tightly associated with FSH-induced atherosclerotic progression. The human monocyte cell line THP-1 was induced by PMA and worked as a macrophage model to detect inflammatory factors and cellular functions. FSH remarkably promoted the expression of IL-1ß in macrophages and strikingly increased the chemotactic migratory capacity of macrophages toward MCP-1, but the promigratory capacity of FSH was attenuated in foam cells. Overall, we revealed that FSH significantly promoted the inflammatory response and migration of macrophages, thereby provoking atherosclerosis development.

20.
Ophthalmic Res ; 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36412621

RESUMO

INTRODUCTION: To assess changes of choroidal thickness (ChT) after administration of 1% atropine for 1 week in myopia, emmetropia, and hyperopia children. METHODS: A total of 235 children aged 4-8 years, which included 46 myopia, 34 emmetropia, and 155 hyperopia patients were recruited and divided into three groups according to the spherical equivalent with the use of 1% atropine twice a day for 1 week. The ChT was measured at baseline and 1 week. RESULTS: In the myopia and emmetropia groups, following administration of 1% atropine gel, the ChT thickened significantly under the fovea (i.e., from 278.29 ± 53.01 µm to 308.24 ± 57.3 µm, P < 0.05; from 336.10 ± 78.60 µm to 353.46 ± 70.22 µm, P < 0.05, respectively) and at all intervals from the fovea, while in the hyperopia group, there was no significant difference in the ChT except the nasal side (P < 0.05) . CONCLUSION: Topical administration of 1% atropine gel for 1 week significantly increased the subfoveal and parafoveal ChT in children with myopia and emmetropia. Atropine did not increase the ChT in hyperopia children, except at the nasal side.

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