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1.
Brain Res Bull ; 177: 73-80, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34555432

RESUMO

Our previous study showed that neuronal apoptosis was significantly increased upon treatment of conditioned medium (CM) from necroptotic astrocytes (NAS), leaving the underlying mechanism unclear. Considering the nutritive and supportive roles of astrocytes, we first examined the neurotrophic phenotype of necroptotic astrocytes with focus on glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF), two important neurotrophic factors, and it was unexpectedly found that the expression of GDNF and BDNF were up-regulated in necroptotic astrocytes in vitro. A question was raised as to whether the functional secreted forms of neurotrophic factors were increased. Considering that extracellular vesicles (EVs) were carriers of secreted substances and their roles in cellular interaction, we isolated EVs from astrocytes and found EVs from normal and necroptotic astrocytes (EVs-NAS) had characteristics of exosomes. We then examined GDNF and BDNF in EVs-NAS, and BDNF was interestingly found as an immature form of pro-BDNF. The expression of pro-BDNF was found to be increased in EVs-NAS, and EVs-NAS had a negative effect on neuronal survival. To verify that whether pro-BDNF was involved in the detrimental effect of EVs-NAS, anti-pro-BDNF antibody was applied, and we found that neuronal apoptosis-induced by EVs-NAS could be significantly attenuated by blocking pro-BDNF, which suggested that necroptotic astrocytes induced neuronal apoptosis partially through EVs-derived pro-BDNF. The data expand our understanding in neurotrophic phenotype of necroptotic astrocytes, and may provide us new strategies targeting on EVs-NAS in treatment of neurological diseases.

2.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 37(9): 775-780, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34533123

RESUMO

Objective To investigate the effect of necrostatin-1 on locomotor recovery after spinal cord injury (SCI) in mice, and to explore the role of apoptosis and M1 type-microglia/macrophage-mediated pro-inflammation in the protective effect. Methods Male C57BL/6 mice were randomly divided into four groups: control group, necrostatin-1 group, SCI model group, necrostatin-1-treated group after SCI, with 20 mice in each. For SCI model group, mice were anesthetized with 10 g/L pentobarbital sodium with a dose of 8 mL/kg. After skin disinfection, T8 laminectomy was performed under operating microscope, and the T8 spinal cord was clearly revealed. The injury model was established with a device designed by our own with the parameter at 0.2 mm-width for 20 seconds. Manual urination was performed once a day. For necrostatin-1-treated group after SCI, 7.8 mg/kg of necrostatin-1 was intravenously administrated at the 1, 2, and 3 days after SCI. For necrostatin-1 group, necrostatin-1 was intravenously injected for three days. Basso Mouse Scale(BMS) score and standardized rump-height index were used to evaluate locomotor function at 1-, 3-, 5-, 7-, 10- and 14-day after injury. To observe cell apoptosis in injured cord, TUNEL staining was performed at 1-, 3-, 7-, and 14-day after injury. Western blot and immunohistochemical staining were performed to detect the expression of inducible nitric oxide synthase (iNOS), a classical marker of M1 type microglia/macrophage. Real time quantitative PCR was used to detect mRNA levels of TNF-α, interleukin-1ß (IL-1ß), IL-18, IL-4, IL-5, and IL-10. Results Necrostatin-1 significantly promoted the locomotor recovery in mice after SCI, reduced cell apoptosis around the SCI area; decreased the protein expression of M1 type microglia/macrophage marker iNOS and the number of iNOS-positive microglia/macrophage, and down-regulated the transcription levels of pro-inflammatory cytokines TNF-α, IL-18, and IL-1ß, while promoting the transcription of anti-inflammatory cytokines IL-4, IL-5, and IL-10. Conclusion Necrostatin-1 significantly promotes locomotor function recovery after SCI in mice by reducing the number of apoptotic cells and inhibiting M1 microglia/macrophages-mediated pro-inflammatory factors.


Assuntos
Microglia , Traumatismos da Medula Espinal , Animais , Apoptose , Imidazóis , Indóis , Macrófagos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Recuperação de Função Fisiológica , Medula Espinal , Traumatismos da Medula Espinal/tratamento farmacológico
3.
Free Radic Biol Med ; 172: 578-589, 2021 08 20.
Artigo em Inglês | MEDLINE | ID: mdl-34242792

RESUMO

Acetaminophen (APAP) is the leading cause of acute liver failure (ALF), which is characterized by GSH depletion, oxidative stress and mitochondrial dysfunction. However, the specific mechanism of APAP-induced ALF remains to be clarified. In this study, we demonstrated that indoleamine 2,3-dioxygenase 1 (IDO1) aggravated APAP-induced ALF associated with excess lipid peroxidation, which was reversed by lipid peroxidation inhibitor (ferrostatin-1). Meanwhile, IDO1 deficiency effectively decreased the accumulation of reactive nitrogen species. Additionally, IDO1 deficiency prevented against APAP-induced liver injury through suppressing the activation of macrophages, thereby reduced their iron uptake and export, eventually reduced iron accumulation in hepatocytes through transferrin and transferrin receptor axis. In summary, our study confirmed that APAP-induced IDO1 aggravated ALF by triggering excess oxidative and nitrative stress and iron accumulation in liver. These results offer new insights for the clinical treatment of ALF or iron-dysregulated liver diseases in the future.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas , Dioxigenases , Falência Hepática Aguda , Acetaminofen/toxicidade , Animais , Doença Hepática Induzida por Substâncias e Drogas/genética , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Dioxigenases/metabolismo , Hepatócitos , Ferro/metabolismo , Fígado/metabolismo , Falência Hepática Aguda/induzido quimicamente , Falência Hepática Aguda/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Estresse Oxidativo
4.
Food Chem ; 339: 127855, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32858384

RESUMO

A magnetic o-hydroxyazobenzene (M-HAzo) porous organic polymer was facilely prepared by a green azo coupling reaction in aqueous solution. The prepared M-HAzo was applied as a new adsorbent for the first time to pre-concentrate phthalate esters (PAEs) from plastic bottled juice, followed by their determination with high performance liquid chromatography-ultraviolet detection. The effects of various parameters, i.e., the mass ratio of the Fe3O4@SiO2 to HAzo, extraction time, ionic strength, pH of the sample, desorption conditions were optimized. Under the optimized conditions, the M-HAzo based method exhibited good performance in terms of linear range (0.3-50.0 µg L-1), detection limit (0.08-0.50 µg L-1), accuracy (recovery of 78.0-115.0%) and repeatability (relative standard deviation of 2.9-7.8%). This work provides a sensitive method for analysis of PAEs at trace levels in drinks, which is featured with high sensitivity, simple operation and environmentally-friendly merit and will have a promising potential in analysis of other organic pollutants.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Ésteres/análise , Magnetismo , Ácidos Ftálicos/química , Compostos Azo/química , Ésteres/isolamento & purificação , Óxido Ferroso-Férrico/química , Sucos de Frutas e Vegetais/análise , Limite de Detecção , Polímeros/química , Reprodutibilidade dos Testes , Dióxido de Silício/química , Extração em Fase Sólida , Espectrofotometria Ultravioleta
5.
Biochem Pharmacol ; 180: 114153, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32679126

RESUMO

Caveolin-1 (Cav-1) is a multifunctional protein and critical for the production of nitric oxide (NO) in intestinal physiological and pathological conditions, but its role in the inflammatory bowel disease(IBD) are still controversial. In this study we tested the hypothesis that Cav-1 could be an important cellular defense against IBD through inhibiting nitrosative stress and mucosal barrier damage. Male wild-type mice and Cav-1 knockout (Cav-1-/-) mice were subjected to 3% dextran sodium sulfate(DSS) for 7d to establish the experimental colitis model. A representative iNOS inhibitor (1400 W) was adopted to suppress the activity of iNOS in parallel group. Body weight and disease activity index were monitored. The colon tissues were evaluated through histological analysis. We found Cav-1 was down-regulated in the colon tissue and accompanied with the increase of iNOS and NO levels after DSS administration. Cav-1-/- mice were greatly increased susceptibility to DSS-induced colitis with the more weight loss and higher disease score than WT mice. Ablation of Cav-1gene significantly resulted in RNS overproduction, tight junctions impaired and inflammation elevated, which aggravated the severity of the intestinal damages. Furthermore, pharmacologic inhibition of iNOS by 1400 W significantly attenuated DSS-induced colitis in both WT and Cav-1-/- groups. Our results revealed an important role of Cav-1 in preventing intestinal nitrosative stress and mucosal barrier damage in the development of DSS-induced colitis.


Assuntos
Caveolina 1/deficiência , Colite/induzido quimicamente , Colite/metabolismo , Sulfato de Dextrana/toxicidade , Mucosa Intestinal/metabolismo , Estresse Nitrosativo/fisiologia , Animais , Colite/prevenção & controle , Mucosa Intestinal/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Estresse Nitrosativo/efeitos dos fármacos
6.
Free Radic Biol Med ; 148: 151-161, 2020 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-31877357

RESUMO

Ferroptosis is a new regulated cells death manner defined as results of iron-dependent accumulation of lipid peroxidation. However, the specific mechanisms of regulating ferroptosis remain unclear. In our present study, we demonstrated that Caveolin-1 (Cav-1) played a central role in protecting hepatocytes against ferroptosis in autoimmunity-mediated hepatitis (AIH). The down-regulated Cav-1 in liver tissues, accompanied by ferroptotic events and RNS production, were contributed to the outcome of ConA-induced hepatic damage, which were rescued by ferrostatin-1 (an inhibitor of ferroptosis) in vivo and in vitro. Additionally, Cav-1 deficiency aggravated ConA-induced hepatocellular death and ferroptosis associated with excessive nitrogen stress response. Short hairpin RNA of Cav-1 in hepatocytes promoted ferroptosis and nitrative stress in response to erastin in vitro, which was ameliorated by Cav-1 over-expression. Meanwhile, administration of the iNOS inhibitor (1400W) or ONOO- scavenger (Fe-TMPyP), diminished reactive nitrogen species (RNS), remarkably reduced hepatocytes ferroptosis and attenuated ConA-induced liver damage. Furthermore, immune inhibition by gadolinium chloride (GdCl3), a well-known Kupffer cell depletor, elevated hepatic Cav-1 but inhibited ferroptosis and nitrative stress under ConA exposure. In conclusion, these data revealed a novel molecular mechanism of ferroptosis with the Cav-1 regulation was essential for pathogenesis of ConA-induced hepatitis. Downstream of Cav-1, RNS-mediated ferroptosis was a pivotal step that drives the execution of acute immune-mediated hepatic damage.


Assuntos
Ferroptose , Caveolina 1/genética , Hepatócitos , Peroxidação de Lipídeos , Nitrogênio
7.
J Chromatogr A ; 1583: 39-47, 2019 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-30459067

RESUMO

This work presents a simple and eco-friendly synthetic approach to fabricate a novel o-hydroxyazobenzene porous organic polymer (HAzo-POP) by diazo-coupling of 2,6-diaminoanthraquinone with m-trihydroxybenzene in aqueous solution. The prepared HAzo-POP possesses good stability and high adsorption capability towards aromatic organic pollutants due to its porous nature, highly conjugated structure and strong hydrogen bonding ability. The HAzo-POP was successfully used for the solid-phase extraction of phenylurea herbicides from six real samples prior to high performance liquid chromatography with ultraviolet detection. The analytical method showed good linearity in the range of 0.5-160.0 ng g-1 for celery, lettuce and tomato samples, and 0.4-160.0 ng mL-1 for milk, soybean milk and juice samples, with low limits of detection in the range from 0.05 to 0.30 ng g-1 (or mL-1). The HAzo-POP has a promising application potential for the adsorption of more aromatic organic compounds.


Assuntos
Compostos Azo/química , Química Verde/métodos , Hidrocarbonetos Aromáticos/química , Polímeros/química , Adsorção , Animais , Compostos Azo/síntese química , Bebidas/análise , Poluentes Ambientais/análise , Herbicidas/análise , Lycopersicon esculentum/química , Leite/química , Porosidade , Reprodutibilidade dos Testes , Extração em Fase Sólida , Espectroscopia de Infravermelho com Transformada de Fourier , Verduras/química , Água/química
8.
J Chromatogr A ; 1572: 20-26, 2018 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-30146376

RESUMO

A covalent organic framework (named as DAAQ-TFP) was prepared via a fast, environmentally-friendly, and easy-to-perform grinding method with 2,6-diaminoanthraquinone (DAAQ) and 1,3,5-triformylphloroglucinol (TFP) as building blocks. The DAAQ-TFP possesses large surface area, high porosity, good thermochemical stability, abundant O atoms and N-H groups, which render it great potential as sorbent for some compounds. It was employed as adsorbent to extract benzoylurea insecticides (BUs) from environmental water, juice, fruit and vegetables samples, followed by their determination with high-performance liquid chromatography (HPLC). The DAAQ-TFP showed a high adsorption capacity for BUs, and the presented method provided a very low limits of detection and high method recoveries. To explore its application potential and also to clarify the possible adsorption mechanisms of the DAAQ-TFP toward BUs, it was tested to extract different types of compounds, proving that the DAAQ-TFP can serve as a superior adsorbent, and adsorption mechanism is mainly based on the π-π stacking and H bonding.


Assuntos
Monitoramento Ambiental/métodos , Análise de Alimentos/métodos , Estruturas Metalorgânicas/química , Resíduos de Praguicidas/análise , Extração em Fase Sólida , Adsorção , Cromatografia Líquida de Alta Pressão , Frutas/química , Inseticidas/análise , Inseticidas/isolamento & purificação , Resíduos de Praguicidas/isolamento & purificação , Verduras/química , Água/química
9.
Materials (Basel) ; 10(12)2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-29194426

RESUMO

As one of the most promising technologies for next-generation lighting and displays, white organic light-emitting diodes (WOLEDs) have received enormous worldwide interest due to their outstanding properties, including high efficiency, bright luminance, wide viewing angle, fast switching, lower power consumption, ultralight and ultrathin characteristics, and flexibility. In this invited review, the main parameters which are used to characterize the performance of WOLEDs are introduced. Subsequently, the state-of-the-art strategies to achieve high-performance WOLEDs in recent years are summarized. Specifically, the manipulation of charges and excitons distribution in the four types of WOLEDs (fluorescent WOLEDs, phosphorescent WOLEDs, thermally activated delayed fluorescent WOLEDs, and fluorescent/phosphorescent hybrid WOLEDs) are comprehensively highlighted. Moreover, doping-free WOLEDs are described. Finally, issues and ways to further enhance the performance of WOLEDs are briefly clarified.

10.
J Chromatogr A ; 1519: 19-27, 2017 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-28888678

RESUMO

A magnetic porphyrin-based porous organic polymer (M-PPOP) with good porosity, high surface area and strong magnetism was prepared and employed as the adsorbent for the magnetic solid phase extraction of the phenylurea herbicides (metoxuron, monolinuron, chlorotoluron, and buturon) from bottled grape juice and tomato samples prior to their determination by high performance liquid chromatography. Under the optimized conditions, the developed method exhibited a good linear range, low limits of detection, low relative standard deviations (<6.8%), good method recoveries between 80.8% and 117%, and high enrichment factors (51-106). For a better elucidation of the adsorption of the M-PPOP towards the analytes, the extraction performance of the M-PPOP for different types of organic compounds including polycyclic aromatic hydrocarbons, phenylurea insecticides, phenylurea herbicides and phenols was studied. The results indicated that the π- stacking, hydrogen-bonding and hydrophobic interactions between the M-PPOP and the analytes played a main role for the adsorption. The results revealed that the M-PPOP material had a great potential for the enrichment of more organic pollutants from real samples.


Assuntos
Análise de Alimentos/métodos , Herbicidas/análise , Herbicidas/isolamento & purificação , Polímeros/síntese química , Extração em Fase Sólida/métodos , Adsorção , Cromatografia Líquida de Alta Pressão , Contaminação de Alimentos , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/isolamento & purificação , Porosidade , Extração em Fase Sólida/instrumentação , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/isolamento & purificação
11.
Mikrochim Acta ; 185(1): 19, 2017 12 05.
Artigo em Inglês | MEDLINE | ID: mdl-29594534

RESUMO

A magnetic mesoporous poly(melamine-formaldehyde) composite (Fe3O4-mPMF) was prepared via grafting poly(melamine-formaldehyde) onto the surface of amino-functionalized magnetite (Fe3O4) nanoparticles. The material was characterized by scanning electron micrography, transmission electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, nitrogen adsorption-desorption isotherms, and thermogravimetric analysis. It has a large surface area, a typical mesoporous structure, and a high thermal stability. It was employed as a magnetic sorbent for the solid phase extraction of the following endocrine disrupting chemicals (EDCs): Bisphenol A, 4-tert-butylphenol, 4-tert-octylphenol and nonylphenol. The EDCs were then quantified by HPLC. Under the optimized conditions, the response to the EDCs is linear in the range of 0.5-100 ng·mL-1, and the limits of detection are 0.02-0.1 ng·mL-1. The high adsorption capability of the Fe3O4-mPMF is mainly attributed to multiple interactions including π-stacking, hydrogen bonding, and hydrophobic interactions. The method was applied to the extraction of EDCs from spiked river water and bottled juice samples. The results demonstrated that the Fe3O4-mPMF is an efficient adsorbent for the extraction of organic compounds with large conjugated π-system, plenty of hydrogen-bonding sites, and strong hydrophobicity. Graphical abstract A magnetic mesoporous polymelamine-formaldehyde composite (Fe3O4-mPMF) was prepared and employed as a magnetic sorbent for the solid phase extraction of endocrine disrupting chemicals from river water and bottled juice samples prior to high-performance liquid chromatographic analysis.

12.
Phytother Res ; 25(7): 1087-94, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21480413

RESUMO

This study was designed to investigate mechanisms of the protective effects of Salvia miltiorrhiza polysaccharide (SMPS) against lipopolysaccharide (LPS)-induced immunological liver injury (ILI) in Bacille Calmette-Guérin (BCG)-primed mice. Two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis showed that three proteins are down-regulated and six proteins are up-regulated by SMPS. SMPS reduces the degree of liver injury by up-regulating the enzymes of the citric acid cycle, namely malate dehydrogenase (MDH) and 2-oxoglutarate dehydrogenase complex. LPS significantly increases nuclear factor kappa B (NF-κB) activation, inducible nitric oxide synthase (iNOS) expression and MDA level in BCG primed mice liver, whereas SMPS treatment protects against the immunological liver injury through inhibition of the NF-κB activation by up-regulation of PRDX6 and the subsequent attenuation of lipid peroxidation, iNOS expression and inflammation.


Assuntos
Fígado/patologia , Polissacarídeos/farmacologia , Salvia miltiorrhiza/química , Animais , Lipopolissacarídeos , Fígado/enzimologia , Fígado/imunologia , Malondialdeído/análise , Camundongos , Camundongos Endogâmicos , NF-kappa B/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Peroxirredoxina VI/metabolismo , Proteômica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Eletroforese em Gel Diferencial Bidimensional
13.
Chin J Integr Med ; 16(6): 510-7, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21110176

RESUMO

OBJECTIVE: To analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats. METHODS: GSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively. RESULTS: A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism. CONCLUSION: Proteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.


Assuntos
Proteômica/métodos , Estresse Psicológico/complicações , Estresse Psicológico/metabolismo , Sequência de Aminoácidos , Animais , Doença Crônica , Modelos Animais de Doenças , Eletroforese em Gel Bidimensional , Fígado/metabolismo , Masculino , Dados de Sequência Molecular , Pré-Albumina/genética , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Restrição Física , Coloração pela Prata , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Síndrome , Transcrição Genética
14.
Zhong Yao Cai ; 31(8): 1197-200, 2008 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-19112901

RESUMO

OBJECTIVE: To study the effects of essential oil extracted from pine needles on HepG2 cell line. METHODS: HepG2 cells were treated with essential oil extracted from pine needles. Cell growth rate was determined with MTF assay, cell morphologic changes were examined under transmission electromicroscope and HE straining. Flow cytometry was used to exmine apoptotic cells. Bcl-2 gene expression was determined by flow cytometry and telomerase activity by TRAP assay. RESULTS: Essential oils from pine needles could not only repress the growth of HepG2 cells significantly, but also induce apoptosis to them. Both dose-effect and time-effect relationship could be confirmed. Typical morphology changes of apoptosis such as nuclear enrichment and karyorrhexis were observed through transmission electromicroscope and HE straining. Telomerase activity was down regulated in the essential oil extracted from pine needles induced apoptotic cells. The expression of bcl-2 gene was suppressed after the essential oil from pine needles treatement. CONCLUSION: The essential oil extracted from pine needles can inhibit cell growth of HepG2 cell line and induce apoptosis, which may associate with inhibition of telomerase activity and bcl-2 may be involved in the regulation of telomerase activity.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Neoplasias Hepáticas/patologia , Pinus/química , Óleos Vegetais/farmacologia , Telomerase/metabolismo , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Citometria de Fluxo , Humanos , Neoplasias Hepáticas/metabolismo , Folhas de Planta/química , Óleos Vegetais/isolamento & purificação , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
15.
Nan Fang Yi Ke Da Xue Xue Bao ; 28(10): 1911-3, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18971199

RESUMO

OBJECTIVE: To observe the effects of bagasse polysaccharide on the immune functions of immunosuppressed mice. METHODS: Immunosuppressed mouse models were established by intraperitoneal injections with cyclophosphamide followed by daily intragastric administration of bagasse polysaccharide. After the treatments, the mice were examined for immune organ weight index, phagocytotic function of the macrophages, delayed type hypersensitivity, serum IgM level following exposure to chicken red blood cells, formation of hemolytic plaques, T cell percentage and lymphocyte transformation. RESULTS: Treatment of the immunosuppressed mice with bagasse polysaccharide at the daily dose of 200 and 400 mg/kg significantly increased the weight of the immune organs, phagocytotic function of the macrophages, delayed type hypersensitivity, serum IgM level against chicken red blood cells, formation of hemolytic plaques, T cell percentage and lymphocyte transformation. CONCLUSION: Bagasse polysaccharide can enhance the immune functions of immunosuppressed mice.


Assuntos
Celulose/química , Hospedeiro Imunocomprometido/imunologia , Ativação Linfocitária/efeitos dos fármacos , Polissacarídeos/farmacologia , Animais , Ciclofosfamida , Feminino , Macrófagos/imunologia , Masculino , Camundongos , Fagocitose/efeitos dos fármacos , Distribuição Aleatória
16.
Beijing Da Xue Xue Bao Yi Xue Ban ; 40(4): 401-4, 2008 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-18677388

RESUMO

OBJECTIVE: To investigate the clinical, pathological characteristics and outcomes of IgA nephropathy in the elderly. METHODS: Seventy patients over age 60 with IgA nephropathy were studied and compared with 82 patients under 60 years in the clinical and pathological features as well as the outcomes. RESULTS: Compared with non-elderly group, elder group patients had higher blood pressure [systolic pressure (142.0+/-20.4) mmHg vs (124.2+/-16.9) mmHg (1mmHg=0.133 kPa), diastolic pressure (83.1+/-11.8) mmHg vs (78.9+/-12.3) mmHg], serum creatinine [(172.7+/-125.8) micromol/L vs (94.4+/-42.5) micromol/L], serum cholesterol[(5.7+/-1.6) mmol/L vs (5.1+/-1.6) mmol/L], 24 hj urinary protein excretion rate [(3.4+/-2.9) g/d vs (1.8+/-2.0) g/d], and the incidence of CKD stages 3-5(64.0% vs 14.6%)(P<0.05). No significant differences were seen in the disease courses, rate of gross hematuria, serum triglyceride and serum IgA level between two groups(P>0.05).Renal pathological investigation showed the chronic lesions were dominated in elder group. There was significant difference in portion of glomerular sclerosis [(19.7+/-20.1)% vs (13.4+/-17.8)%], renal tubule atrophy (>1 score,34.2% vs 25.6%), interstitial fibrosis (>1,score 34.2% vs 18.2%), and arteriolosclerosis (>2 score,20.0% vs 8.5%) between two groups (P<0.05). However, there were no significant difference in proportion of mesangial proliferation, crescent and interstitial inflammatory cell infiltration (P>0.05). After a mean post-biopsy follow-up of (34.6+/-33.3) months, the 3-year and the 5-year renal survival rates for elder group were 74.6% and 62.2%, respectively, which were lower than those of non-elder group (100% and 92.9%, P=0.002). CONCLUSION: Elder patients with IgA nephropathy were more likely to suffer from hypertension, hyperlipidemia, renal insufficiency and chronic pathologic lesions, which might be the risk factors for the patient's unfavorable prognosis.


Assuntos
Glomerulonefrite por IGA/patologia , Hiperlipidemias/complicações , Hipertensão/complicações , Adulto , Fatores Etários , Idoso , Biópsia por Agulha , Feminino , Glomerulonefrite por IGA/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Fatores de Risco , Resultado do Tratamento
17.
Int J Biol Macromol ; 43(2): 170-5, 2008 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-18571229

RESUMO

This study was designed to evaluate the hepatoprotective effects of S. miltiorrhiza polysaccharides (SMPS) in immunological liver injury induced by Bacille-Calmette-Guerin (BCG) and lipopolysaccharide (LPS) in mice. SMPS effectively improved the liver index, spleen index and thymus index, reduced the serum levels of alanine aminotransferase, aspartate aminotransferase and nitric oxide, and restored liver homogenate contents of tumor necrosis factor-alpha and interleukin-1beta. The histopathological analysis suggested that SMPS reduced the degree of liver injury. The results suggest that SMPS play a protective role against immunological liver injury, which may have important implications for our understanding on the immunoregulatory mechanisms of polysaccharides.


Assuntos
Medicamentos de Ervas Chinesas/química , Hepatopatias/prevenção & controle , Fígado/efeitos dos fármacos , Extratos Vegetais/química , Polissacarídeos/farmacologia , Salvia miltiorrhiza/química , Alanina Transaminase/sangue , Análise de Variância , Animais , Aspartato Aminotransferases/sangue , Doença Hepática Induzida por Substâncias e Drogas , Interleucina-1beta/metabolismo , Lipopolissacarídeos/toxicidade , Fígado/imunologia , Fígado/lesões , Hepatopatias/sangue , Hepatopatias/metabolismo , Masculino , Camundongos , Mycobacterium bovis/imunologia , Óxido Nítrico/sangue , Baço/efeitos dos fármacos , Timo/efeitos dos fármacos , Fator de Necrose Tumoral alfa/metabolismo
18.
Nan Fang Yi Ke Da Xue Xue Bao ; 28(3): 494-6, 2008 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-18359723

RESUMO

OBJECTIVE: To investigate the protective effects of Hongbeiyegen (HBYG) against immunological liver injury induced by bacille Calmette-Guerin (BCG) and lipopolysaccharide (LPS). METHODS: Immunological liver injury was induced in rats by BCG and LPS injected via the tail vein. The liver index, thymus index and spleen index were calculated and the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and nitric oxide (NO) and liver homogenate contents of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were determined. RESULTS: HBYG significantly improved the liver index, thymus index and spleen index, and reduced the serum levels of ALT, AST and NO, and as the liver homogenate contents of TNF-alpha and IL-1beta. CONCLUSION: HBYG offers obvious protective effects against immunological injury liver in mice.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Euphorbiaceae/química , Hepatopatias/prevenção & controle , Fígado/efeitos dos fármacos , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Doença Hepática Induzida por Substâncias e Drogas , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Interleucina-1beta/metabolismo , Lipopolissacarídeos , Fígado/metabolismo , Fígado/patologia , Hepatopatias/imunologia , Masculino , Camundongos , Camundongos Endogâmicos , Mycobacterium bovis , Óxido Nítrico/sangue , Fitoterapia , Raízes de Plantas/química , Resultado do Tratamento , Fator de Necrose Tumoral alfa/metabolismo
19.
Beijing Da Xue Xue Bao Yi Xue Ban ; 39(5): 458-61, 2007 Oct 18.
Artigo em Chinês | MEDLINE | ID: mdl-17940559

RESUMO

OBJECTIVE: To compare the clinicopathological findings and prognosis of Henoch-Schonlein nephritis (HSPN) and IgA nephropathy (IgAN) in adults. METHODS: We enrolled 31 patients with HSPN and 62 patients with IgAN in the present study. They were followed up for 47+/-23 months and 47+/-20 months respectively, and their clinical manifestations and renal pathological findings were collected. Renal pathological changes were semiquantitatively graded. RESULTS: The clinical manifestations, including hypertension, excretion of serum creatinine and urinary protein, were similar in patients with HSPN and IgAN [38.7% vs 27.4%, (121+/-164) vs (106+/-43) micromol/L, (3.2+/-3.1) vs (2.8+/-2.9) g/d, P>0.05]. Renal pathological investigation showed endothelial proliferation in 40.6% (13/31) of HSPN patients and 19.4% (12/62) of IgAN patients and the difference was significant (P=0.021). In patients with IgAN, the tubulointerstitial chronicity index was higher than that in HSPN (2 vs 1, P=0.009), but there were no statistically significant differences in crescent formation(including segmental glomerular necrosis) and glomerular sclerosis(1 vs 0, 1 vs 1, P>0.05). In patients with HSPN capillary wall staining for IgA was more frequently found than in IgAN (71.0% vs 43.5%, P=0.013). With creatinine level doubling as the end point, the follow-up data indicated that the renal survival was 87.1% in HSPN and 91.9% in IgAN and there was no statistically significant difference between HSPN and IgAN (P=0.481). CONCLUSION: Although significant pathological difference was found between HSPN and IgAN, the renal clinical manifestations and long term outcome were similar between the two diseases in adults.


Assuntos
Glomerulonefrite por IGA/patologia , Nefrite/patologia , Púrpura de Schoenlein-Henoch/patologia , Adulto , Feminino , Glomerulonefrite por IGA/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Nefrite/diagnóstico , Nefrite/etiologia , Prognóstico , Púrpura de Schoenlein-Henoch/complicações , Púrpura de Schoenlein-Henoch/diagnóstico , Estudos Retrospectivos , Adulto Jovem
20.
Nan Fang Yi Ke Da Xue Xue Bao ; 27(2): 153-5, 2007 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-17355923

RESUMO

OBJECTIVE: To observe the therapeutic effect of Hongbeiyegen [the root of Alchornea trewioides(Benth.) Muell.-Arg.] on alcohol-induced liver fibrosis (AF) in rats and explore its mechanism. METHODS: In rats with AF, the serum levels of transforming growth factor beta1 (TGFbeta1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were detected along with examination of the changes in serum hyaluronic acid (HA), laminin (LN), procolagen type III (PC III), collagen type IV (C IV), glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminase (AST) levels. RESULTS: Compared with the control group, Hongbeiyegen could significantly reduce the levels of TGFbeta1, TIMP-1, HA, LN, PC III, CIV, ALT and AST in rats with AF. CONCLUSION: Hongbeiyegen can relieve and ameliorate liver fibrosis possibly by inhibiting the expression of TGFbeta1 and TIMP-1.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Euphorbiaceae/química , Cirrose Hepática Experimental/tratamento farmacológico , Raízes de Plantas/química , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Colágeno Tipo III/sangue , Colágeno Tipo IV/sangue , Etanol , Feminino , Ácido Hialurônico/sangue , Laminina/sangue , Cirrose Hepática Experimental/sangue , Cirrose Hepática Experimental/induzido quimicamente , Masculino , Fitoterapia , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Inibidor Tecidual de Metaloproteinase-1/sangue , Fator de Crescimento Transformador beta1/sangue
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