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1.
Photodiagnosis Photodyn Ther ; : 102622, 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34775066

RESUMO

OBJECTIVE: To evaluate the effect of potassium iodide (KI) addition on antimicrobial photodynamic therapy (aPDT) mediated by red laser (λ = 660 nm) and methylene blue in Streptococcus mutans biofilm model. METHODS: S. mutans biofilms were cultured in 96-well plates containing BHI broth with 1% sucrose for 18 h, 10% CO2 and 37°C and divided in groups (n = 3, in triplicate): C (NaCl 0.9%); CX (0.2% chlorhexidine); P (photosensitizer); KI (10, 25 and 50 mM); PKI (10, 25 and 50 mM); L (L 1: : 100 J/cm2, 9 J; L2: 200 J/cm2, 18 J); PL (photosensitizer + L1 or L2); KIL (KI at 10, 25 and 50 mM + L1 or L2); and PKIL (photosensitizer + 10, 25 and 50 mM KI + L1 or L2). Biofilms were submitted to three pre-irradiation (PI) times (5, 10, and 15 min). After the treatments, microbial counting's reduction was analyzed by Kruskal-Wallis and post-hoc Dunn's tests, respectively, and the interaction between light parameters and the PI times by two-way ANOVA (p < 0.05). RESULTS: The S. mutans viability significantly reduced in all aPDT groups, in the presence or absence of KI (p < 0.05). For all PI times, PKIL groups (10, 25, and 50 mM) significantly differed from PL groups (p < 0.05) with a reduction of 9.0 logs reached at 50 mM of KI with 15 min of PI, irradiated at 18 J. We found no significant interaction between PI time and irradiation (p > 0.05). CONCLUSION: Addition KI to TFDA mediated by methylene blue and red laser promoted an additional effect in reducing the microbial viability of S. mutans biofilm.

2.
J Dent Child (Chic) ; 88(2): 140-143, 2021 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-34321147

RESUMO

Aggressive periodontitis is a disease that causes severe destruction of periodontal tissues, showing early development and rapid progression in both primary and permanent dentitions. Due to familial aggregation, children of parents with periodontitis are considered to be at higher risk for disease occurrence, which suggests that they should be evaluated and monitored as early as possible. The purpose of this case report is to describe aspects related to early diagnosis of periodontitis in two children and their relationship with the parent's periodontal condition, exploring the familial component as a crucial factor that can lead to an early diagnosis and better clinical management in their offspring.


Assuntos
Periodontite Agressiva , Doenças da Gengiva , Periodontite Agressiva/diagnóstico , Periodontite Agressiva/tratamento farmacológico , Periodontite Agressiva/genética , Antibacterianos/uso terapêutico , Criança , Dentição Permanente , Humanos
3.
Photodiagnosis Photodyn Ther ; 34: 102283, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33813017

RESUMO

OBJECTIVE: To test the effect of antimicrobial photodynamic therapy (A-PDT) on the oral biofilm formed with early colonizing microorganisms, using the photosensitizer methylene blue coupled with ß-cyclodextrin nanoparticles and red light sources laser or LED (λ =660 nm). METHODS: The groups were divided into (n = 3, in triplicate): C (negative control, 0.9 % NaCl), CX (positive control, 0.2 % chlorhexidine), P (Photosensitizer/Nanoparticle), L (Laser), LED (light-emitting diode), LP (Laser + Photosensitizer/Nanoparticle) and LEDP (LED + Photosensitizer/Nanoparticle). A multispecies biofilm composed ofS. gordonii, S. oralis, S. mitis, and S. sanguinis was grown in microplates containing BHI supplemented with 1% sucrose (w/v) for 24 h. Light irradiations were applied with a laser at 9 J for 90 s (320 J/cm2), or with LED, at 8.1 J for 90 s (8.1 J/cm2). The microbial reduction was assessed by counting viable biofilm microorganisms in selective culture media, before and after the treatments. Data normality was assessed by the Shapiro-Wilk test, and the results were submitted to Kruskal-Wallis analysis, followed by Dunn's test, with a significance level of 5%. RESULTS: The groups LP and LEDP were able to significantly reduce the biofilm microorganism counts by as much as 4 log10 times compared to the negative control group (p < 0.05) and did not statistically differ from the positive control group (CX) (p > 0.05). CONCLUSION: The A-PDT mediated by encapsulated ß-cyclodextrin methylene blue irradiated by Laser or LED was effective in the microbial reduction of multispecies biofilm composed of early colonizing microorganisms.


Assuntos
Anti-Infecciosos , Fotoquimioterapia , beta-Ciclodextrinas , Biofilmes , Azul de Metileno/farmacologia , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Streptococcus mutans
4.
Sci Rep ; 11(1): 7496, 2021 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-33820926

RESUMO

This quasi-experimental study sought to investigate if the mechanical control of biofilm (3-times-a-day) modifies the saliva's ability to buffer the oral environment after 20% sucrose rinse (SR20%) in children with early childhood caries (ECC). Here, SR20% reduced the saliva's pH in both groups and the mechanical control of biofilm had a greater effect on this parameter after SR20% in CF children. The mechanical control of biofilm evidenced a higher buffering capacity in CF children before SR20%, which was not observed after SR20%. Otherwise, the absence of mechanical control of biofilm showed that buffering capacity was comparable in the two groups before SR20%, whereas after SR20% the saliva's buffering capacity of CF children was higher than ECC children. When biofilm was mechanically controlled, carbonic anhydrase VI activity did not change after SR20% whereas the absence of mechanical control of biofilm reduced this enzyme activity after SR20%. In conclusion, the mechanical control of biofilm did not change saliva's ability to buffer the oral environment after SR20% in children with ECC. On the other hand, CF children appeared to regulate more effectively the saliva's pH than ECC children while the absence of mechanical control of biofilm mediated their pH-modifying ability after SR20%.


Assuntos
Biofilmes , Cárie Dentária/microbiologia , Saliva/microbiologia , Sacarose/efeitos adversos , Tampões (Química) , Anidrases Carbônicas/metabolismo , Criança , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino , Saliva/enzimologia , Salivação/fisiologia , alfa-Amilases/metabolismo
5.
Photodiagnosis Photodyn Ther ; 33: 102093, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33212267

RESUMO

BACKGROUND: Herpes Simplex Virus Type 1 (HSV-1) is one of the most widespread infections that can effect the orofacial region. Recurrent infection is considered a life-long oral health problem, leading to pain, discomfort, and social restriction due to esthetic features when active. Effective therapies are needed. This study aimed to compare photodynamic therapy (PDT), Topical Acyclovir (AC), and the association of both in the healing process and self-reported symptomologies of HSV-1 recurrences. METHODS: Patients were randomly assigned into 3 groups (n = 25): PDT (low-power laser, 660 nm, 40 mW, 120 J/cm2, 4.8 J, 120 s per point) and methylene blue (0.005 %) as photosensitizer; AC (5%); PDT + AC.Data concerning lesion size, healing time, and self-reported healing parameters, such as pain, tingling, and edema were taken every day up to complete healing for all studied groups. RESULTS: There was no significant difference in healing time and pain between groups. AC group showed a significant minor reduction of the lesion compared to the AC-PDT group on day 1. Regarding edema and tingling, the comparison of treatments showed a statistical difference only on day 1, where PDT showed better results. CONCLUSION: With all the limitations of this study, it can be concluded that only on day 1 PDT showed positive effects in the treatment of herpes lesions in comparison to AC.


Assuntos
Herpes Labial , Herpesvirus Humano 1 , Fotoquimioterapia , Aciclovir/uso terapêutico , Herpes Labial/tratamento farmacológico , Humanos , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Recidiva
6.
RGO (Porto Alegre) ; 69: e20210023, 2021. graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1287739

RESUMO

ABSTRACT The aim of the current study is to present a treatment approach in a case of severe early childhood caries (ECC). A 5-year-old girl was referred to the Pediatric Dentistry Division with multiple premature tooth loss, masticatory difficulties, prolonged breast-feeding, and low quality of life, who was diagnosed with severe ECC. A three-phased treatment plan was implemented: 1. Disease control - consisting of behavioral changes in oral hygiene habits, diet guidance, professional biofilm removal followed by fluoride application and temporary restorations; 2. Infection control - teeth extractions and pulpotomy; and 3. Functional rehabilitation - direct and semi-indirect resin restorations, a removable partial and total prostheses in the lower and upper jaws, respectively. In conclusion, the proposed multidisciplinary approach resulted in a positive impact on the patient's nutrition and growth, speech production, communication, self-image, and social functioning leading to an improved quality of life.


RESUMO O objetivo do presente estudo é apresentar uma abordagem de tratamento em um caso de cárie na primeira infância (CPI). Uma menina de 5 anos foi encaminhada à área de Odontopediatria com múltiplas perdas dentárias precoces, dificuldades mastigatórias, amamentação prolongada e baixa qualidade de vida, com diagnóstico de CPI severa. Um plano de tratamento em três fases foi implementado: 1. Controle da doença - consistindo em mudanças comportamentais nos hábitos de higiene bucal, orientação alimentar, remoção profissional do biofilme seguida de aplicação de flúor e restaurações provisórias; 2. Controle de infecção - exodontias e pulpotomia; e 3. Reabilitação funcional - restaurações diretas e semi-indiretas de resina, próteses parcial e total removíveis nos maxilares inferior e superior, respectivamente. Em conclusão, a abordagem multidisciplinar proposta resultou em um impacto positivo na nutrição e crescimento do paciente, produção da fala, comunicação, autoimagem e socialização, levando a uma melhoria da qualidade de vida.

7.
Front Pediatr ; 8: 588965, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33363062

RESUMO

This brief communication assessed whether there was any relationship between the counts of lactobacilli (LB) and mutans streptococci (MS) in the oral cavity and intestine of obese and eutrophic children with early childhood caries (ECC). Seventy-eight preschoolers were assigned into the following groups: 1. obese children with ECC (OECC), 2. eutrophic children with ECC (EECC), 3. obese caries-free children (OCF), and 4. eutrophic caries-free children (ECF). The diagnosis of obesity and ECC was based on the World Health Organization criteria. Dental plaque and fecal samples were collected to assess the counts of MS and LB using selective media. Data were evaluated by Poisson regression analysis, Wilcoxon test, and Sign test. Microbial indicators of ECC in obese children were MS counts in the intestine [rate ratio (RR): 4.38] and presence of LB in the oral cavity (RR: 2.12). The indicators in eutrophic children were MS levels and the presence of LB, both in the oral cavity (RR: 6.35/1.50) and intestine (RR: 2.35/2.38) (p < 0.05). The comparison between MS levels in the mouth and in the intestine revealed significant differences only in the ECF group (p = 0.04). Regarding LB presence in the mouth vs. in the intestine, except for the OCF group (p = 0.03), no other statistical differences were found. Our preliminary findings highlighted that the levels of MS and the presence of LB in the oral cavity, as well as in the lower gastrointestinal tract were associated with ECC. Moreover, obesity was found to influence this relationship.

8.
J Clin Exp Dent ; 12(3): e209-e214, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32190189

RESUMO

Background: This research aimed to evaluate the salivary concentrations of fluoride (F-), calcium (Ca2+), and phosphate (Pi) after brackets bonding, and to identify the role of [F-], [Ca2+], and [Pi] on the development of active caries lesion (ACL) in individuals under fixed orthodontic treatment. Material and Methods: A longitudinal investigation with twenty-two individuals from 11 to 22 years of age was performed in four phases (baseline and after 1, 3, and 6 months). Analyses were carried out considering the salivary concentration of [F-], [Ca2+], and [Pi], as well as the caries index. Data were analyzed using the Friedman test, followed by the Wilcoxon test and the multivariate Cox model (p≤0.05). Results: 1 and 3 months after appliance bonding, the [Ca2+] was statistically lower than after 6 months (p<0.0083). On the other hand, salivary [F-] and [Pi] did not show any significant difference during the follow-up. The Cox model demonstrated that the increase of 1 µg/mL in Ca2+ decreased the risk of ACL development by 27%. In conclusion, the levels of Ca2+ changed during orthodontic treatment. Conclusions: A high Ca2+ level in the saliva is a protective factor for ACL development over time. Key words:Adolescents, bioinorganic chemistry, dental caries, orthodontic appliances.

9.
Clin Cosmet Investig Dent ; 12: 71-78, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32210634

RESUMO

Objective: This longitudinal study aimed to investigate if the plaque fluoride (PF) concentration can predict the early childhood caries onset. Design: As part of a larger study, 188 preschoolers were clinically evaluated for early childhood caries diagnosis, at baseline and after 1-year follow-up. After that, the final sample comprised: 1. children who had already caries at baseline (decayed, missing or filled surfaces, as well as white chalky white spot lesions adjacent to gingival margins) and developed at least one more cavitation after one year (n=16), and 2. children who never had or developed any caries lesions, including active white spots lesions (n=15). Before the clinical examinations, dental plaque was collected. PF concentration was determined with an ion-specific electrode. A chart was used to estimate the mean daily sugar exposure. The results were statistically analyzed by Spearman correlation and logistic regression analyses (α=0.05). Results: After one year, a positive significant correlation between caries development and liquid sucrose, total sugar and total sucrose consumption increments was found (p<0.05). Moreover, the solid sugar, solid sucrose and total sugar exposure at baseline were positively correlated with the presence of dental plaque at follow-up (p<0.05). To top it all, children with PF concentrations ≤0.1 µg/mg at baseline were 10 times more likely to develop caries. Conclusion: In conclusion, this study demonstrated for the first time in vivo that low PF concentration is a predictor of caries development in primary teeth.

10.
Photodiagnosis Photodyn Ther ; 28: 226-233, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546042

RESUMO

OBJECTIVE: This study evaluated the effect of hydrogen peroxide addition on ß-cyclodextrin-conjugated methylene blue in antimicrobial photodynamic therapy(a-PDT) in S. mutans biofilm model using laser or light emitting diode (LED) (λ = 660 nm). METHODS: A preliminary assay was performed to evaluate the cytotoxicity of hydrogen peroxide in oral fibroblasts by the colorimetric method (MTT). Afterwards, groups were divided into (n = 3, in triplicate): C (negative control), CX - chlorhexidine 0.2% (positive control), P (methylene blue/ß-cyclodextrin), H (Hydrogen Peroxide at 40 µM), PH, L (Laser), LP, LH (Laser+Hydrogen Peroxide), LPH, LED, LEDP, LEDH, and LEDPH. The biofilm was formed in 24 h with BHI + 1% sucrose (w/v). Light irradiations were conducted with laser, 9 J, 323 J/cm2, 113 s or with LED, 8.1 J, 8.1 J/cm2 for 90 s. Microbial reduction was evaluated by counting the viable microorganisms of the biofilm after the respective treatments, in a selective culture medium, and laser confocal microscopy evaluation. RESULTS: LP, LH, LPH, LEDP, LEDH, and LEDPH groups statistically reduced the counts of S.mutans compared with the C group and the log reductions were of 1.87, 1.94, 2.19, 0.91, 0.92, and 1.33, respectively; the addition of hydrogen peroxide did not potentiate the microbial reductions (LPH and LEDPH) compared with the LP and LEDP groups. CONCLUSION: The association of hydrogen peroxide with the conjugated ß-cyclodextrin nanoparticle as photosensitizer did not result in an enhanced effect of a-PDT; hydrogen peroxide behaved as a photosensitizer, since it reduced the number of S. mutans when associated with laser light.


Assuntos
Biofilmes/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Azul de Metileno/farmacologia , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Streptococcus mutans/efeitos dos fármacos , beta-Ciclodextrinas/farmacologia , Viabilidade Microbiana/efeitos dos fármacos , Microscopia Confocal , Nanopartículas
11.
Photobiomodul Photomed Laser Surg ; 37(9): 567-573, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31411536

RESUMO

Objective: This study aimed to investigate if ß-cyclodextrin nanoparticles potentiate the photodynamic antimicrobial chemotherapy (PACT) effects in single and microcosm oral biofilms using methylene blue (MB) and a red laser. Background data: Studies of PACT have demonstrated promising effects; however, the association of nanoparticles with photosensitizers could enhance the antimicrobial result. Materials and methods: Biofilms were grown on enamel blocks either with Streptococcus mutans or in a microcosm model (salivary microorganisms) supplemented with sucrose. PACT using 50 µM MB associated or not with 32 µM encapsulated ß-cyclodextrin with MB for 5 min, followed by irradiation with red laser (λ = 660 nm, 320 J/cm2), was conducted and the counts of viable microorganisms in proper selective media were determined. Data were analyzed by one-factor ANOVA, followed by Tukey's test, or Kruskal-Wallis test, followed by Dunn's post hoc test, all with a significance level of 5%. Results: In the single-species biofilm model, a significant reduction in S. mutans counts was found for all groups when light was present. In the microcosm biofilm model, no significant difference was found among the groups for total streptococci, but a significant reduction of S. mutans was observed for the PACT group of encapsulated ß-cyclodextrin+MB. However, no statistically significant difference was observed among the PACT groups. Conclusions: PACT with ß-cyclodextrin mediated with MB associated with a red laser reduced S. mutans in microcosm biofilms. However, the presence of ß-cyclodextrin nanoparticles did not potentiate the PACT effects in single or microcosm oral biofilms.


Assuntos
Biofilmes/efeitos dos fármacos , Nanopartículas , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Streptococcus mutans/efeitos dos fármacos , beta-Ciclodextrinas/farmacologia , Biofilmes/efeitos da radiação , Azul de Metileno , Viabilidade Microbiana , Streptococcus mutans/efeitos da radiação
12.
J. Health Biol. Sci. (Online) ; 7(2): 166-171, abr.-jun. 2019.
Artigo em Português | LILACS | ID: biblio-1005730

RESUMO

Introdução: cárie dentária é um problema de saúde pública e compromete a qualidade de vida dos indivíduos. O uso de dentifrícios fluoretados associado à escovação mecânica vem sendo bastante estudado como controle da doença. Objetivo: avaliar o conhecimento dos pais e/ou responsáveis por crianças frequentadoras da Clínica Odontológica da Faculdade Faesa (ES), sobre a higienização bucal e o uso de dentifrício fluoretado. Métodos: foram analisados 46 questionários, contendo 12 questões objetivas. Resultados: a análise estatística descritiva dos dados demonstrou que 78,3% das crianças utilizavam dentifrício fluoretado, 93,5% dos pais não sabiam a concentração de fluoreto presente no creme dental das crianças, desconheciam o momento para se introduzir o dentifrício fluoretado; 60,9% dos pais não sabiam o que poderia ocorrer a partir da ingestão do dentifrício fluoretado e 71,4% responderam que a função do fluoreto era prevenir a cárie. Para 50,0% das famílias, a introdução do creme dental fluoretado foi apenas a partir do segundo ano de vida e apenas 4,4% sabiam que as lesões de cárie se iniciam pela presença de uma mancha branca. Conclusão: pais e/ou responsáveis possuem conhecimento sobre a função do fluoreto, a quantidade de dentifrício que deve ser utilizada em cada faixa etária; porém, desconhecem a concentração de fluoreto presente no creme dental, não sabem o momento correto para introduzir o dentifrício fluoretado ou os riscos de desenvolvimento de fluorose dentária.


Introduction: Caries is considered a public health problem that compromise individual's quality of life. The use of fluoridated dentifrices together with mechanical brushing have been widely studied as a control of the disease. This research evaluated the level of knowledge of parents and/or guardians of children attending the Dental Clinic of Faesa College (Vitória, ES), regarding information about dental hygiene, use of toothpaste. Methods: We analyzed 46 questionnaires, containing 12 objective questions. Results: The descriptive statistical analysis showed that 78.3% of the children used fluoride dentifrice, 93.5% of the parents did not know the fluoride concentration present in the children's toothpaste, they did not know when to introduce the fluoride dentifrice, 60.9% of the parents did not know what could occur from the ingestion of the fluoride dentifrice and 71.4% answered that fluoride function was to prevent caries. For 50.0% of the families, the introduction of the fluoride toothpaste was only from the second year of life and only 4.4% knew that the caries lesions are initiated by the presence of a white spot. Conclusion: Parents and/or caregivers have knowledge about the function of fluoride, the amount of toothpaste that should be used in each age group; however, are unaware of the fluoride concentration present in the toothpaste, do not know the correct time to introduce the fluoride dentifrice or the risks of developing dental fluorosis.


Assuntos
Cárie Dentária , Fluoretos , Dentifrícios
13.
Caries Res ; 53(3): 296-304, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30317232

RESUMO

OBJECTIVE: We aimed to evaluate whether radiotherapy causes changes in the mineral composition, hardness, and morphology of enamel and dentin of primary teeth. MATERIALS AND METHODS: Thirty specimens of primary teeth were subjected to radiotherapy. At baseline and after 1,080, 2,160, and 3,060 cGy, the specimens were subjected to microhardness, FT-Raman spectroscopy, and scanning electron microscopy (SEM) analysis. The pH of artificial saliva was determined, as were the calcium and phosphate concentrations. The data were subjected to the Shapiro-Wilk normality test, showed a nonnormal distribution, and were compared by the Kruskal-Wallis test. RESULTS: The results showed that the microhardness of the enamel surface decreased after 2,160 cGy (281.5 ± 58 kgf/mm2) when compared to baseline (323.6 ± 59.5 kgf/mm2) (p = 0.045). For dentin, the surface hardness decreased after 1,080 cGy (34.9 ± 11.4 kgf/mm2) and 2,160 cGy (26 ± 3.5 kgf/mm2) when compared to baseline (56.5 ± 7.7 kgf/mm2) (p < 0.0001). The mineral and organic contents of phosphate (p < 0.0001), carbonate (p < 0.0001), amide (p = 0.0002), and hydrocarbons (p = 0.0031) of enamel decreased after 3,060 cGy (5,178 ± 1,082, 3,868 ± 524, 999 ± 180, and 959 ± 168 kgf/mm2, respectively). For dentin, we noticed a growing increase in phosphate v2, amide, and hydrocarbon content after 1,080 cGy (8,210 ± 2,599, 5,730 ± 1,818, and 6,118 ± 1,807 kgf/mm2, respectively) and 2,160 cGy (1,0071 ± 2,547, 7,746 ± 1,916, and 8,280 ± 2,079 kgf/mm2, respectively) and a reduction after 3,060 cGy (6,782 ± 2,175, 3,558 ± 1,884, and 3,565 ± 1,867 kgf/mm2, respectively) (p < 0.0001). SEM images showed cracks on enamel and degradation of peritubular dentin. CONCLUSION: We concluded that radiotherapy caused a reduction in surface hardness, changed mineral and organic composition, and promoted morphological changes on the enamel and dentin of primary teeth.


Assuntos
Esmalte Dentário/química , Dentina/química , Minerais/química , Radioterapia/efeitos adversos , Dente Decíduo/efeitos da radiação , Esmalte Dentário/efeitos da radiação , Dentina/efeitos da radiação , Dureza , Humanos , Técnicas In Vitro , Microscopia Eletrônica de Varredura
14.
Photomed Laser Surg ; 35(8): 415-420, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28783465

RESUMO

OBJECTIVE: Analyze the effect of photobiomodulation in the prevention of tooth sensitivity after in-office dental bleaching. BACKGROUND DATA: Tooth sensitivity is a common clinical consequence of dental bleaching. Therapies for prevention of sensitivity have been investigated in literature. MATERIALS AND METHODS: This study was developed as a randomized, placebo blind clinical trial. Fifty patients were selected (n = 10) and randomly divided into five groups: (1) control, (2) placebo, (3) laser before bleaching, (4) laser after bleaching, and (5) laser before and after bleaching. Irradiation was performed perpendicularly, in contact, on each tooth during 10 sec per point in two points. The first point was positioned in the middle of the tooth crown and the second in the periapical region. Photobiomodulation was applied using the following parameters: 780 nm, 40 mW, 10 J/cm2, 0.4 J per point. Pain was analyzed before, immediately after, and seven subsequent days after bleaching. Patients were instructed to report pain using the scale: 0 = no tooth sensitivity, 1 = gentle sensitivity, 2 = moderate sensitivity, 3 = severe sensitivity. RESULTS: There were no statistical differences between groups at any time (p > 0.05). More studies, with others parameters and different methods of tooth sensitivity analysis, should be performed to complement the results found. CONCLUSIONS: Within the limitation of the present study, the laser parameters of photobiomodulation tested in the present study were not efficient in preventing tooth sensitivity after in-office bleaching.


Assuntos
Sensibilidade da Dentina/prevenção & controle , Terapia com Luz de Baixa Intensidade/métodos , Clareadores Dentários/efeitos adversos , Clareamento Dental/efeitos adversos , Adulto , Consultórios Odontológicos , Sensibilidade da Dentina/induzido quimicamente , Feminino , Humanos , Masculino , Medição da Dor , Valores de Referência , Medição de Risco , Método Simples-Cego , Clareamento Dental/métodos , Clareadores Dentários/química , Resultado do Tratamento , Adulto Jovem
15.
Int J Paediatr Dent ; 27(6): 540-550, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28247450

RESUMO

BACKGROUND: Previous studies have shown during the orthodontic treatment, the prevalence of active caries lesions (ACL) in 30 to 70% of patients. AIM: To evaluate the effect of orthodontic treatment on salivary properties considering caries development. DESIGN: Twenty two individuals (11-22 years) were assessed regarding caries, oral hygiene, sugar exposure, salivary flow rate (SFR), buffering capacity, pH, carbonic anhydrase VI (CA VI) and amylase activity, at baseline, 1, 3 and 6 months after the orthodontic appliances placement. Caries index was determined by Nyvad criteria. CA VI and amylase activities were performed by zymography and ELISA, respectively. RESULTS: Buffering capacity decreased after 3 months (P < 0.0001) and showed positive correlation with pH at baseline and after 1 month in individuals who did not develop active caries lesions. Amylase activity decreased after 1 and 6 months (P = 0.0003) and presented positive correlation with CA VI in patients who developed ACL. SFR increased after 1 month (P = 0.0283) and showed positive correlation with CA VI activity after 3 months in individuals who did not develop ACL. Salivary pH showed negative correlation with CA VI after 1 month in patients who did not develop ACL. ACL were firstly diagnosed after 3 months. CONCLUSIONS: The present study demonstrated that saliva of individuals under orthodontic treatment is subjected to changes in properties that have implications on the onset of ACL.


Assuntos
Cárie Dentária/etiologia , Aparelhos Ortodônticos , Saliva/fisiologia , Adolescente , Amilases/metabolismo , Biofilmes , Anidrases Carbônicas/metabolismo , Criança , Dieta , Açúcares da Dieta , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino , Saliva/química , Saliva/enzimologia , Dente/microbiologia , Adulto Jovem
16.
Int J Paediatr Dent ; 27(3): 174-182, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27430359

RESUMO

AIM: This longitudinal study investigated the relationship among early childhood caries (ECC), α amylase, carbonic anhydrase VI (CA VI), and the presence of visible biofilm, besides detecting if these variables could predict risk for ECC. DESIGN: One hundred children were divided into two groups: caries group (n = 45) and caries-free group (n = 55). Visible biofilm on maxillary incisors was recorded, followed by caries diagnosis in preschoolers at baseline and at follow-up. Saliva samples were collected, and activities of CA VI and α amylase were determined. Data normality was assessed by Shapiro-Wilk test and then Mann-Whitney, Spearman correlation, and chi-square tests followed by multiple logistic regression analysis (α = 0.05, 95% confidence interval). RESULTS: CA VI activity was significantly higher in saliva of children with caries (P ≤ 0.05), and α amylase activity was significantly higher in saliva of caries-free children (P < 0.0001). Children with α amylase activity in saliva lower than 122.8 U/mL (OR = 3.33 P = 0.042) and visible biofilm on maxillary incisors (OR = 3.6 P = 0.009) were more likely to develop ECC than caries-free children. A negative correlation between caries and α amylase activity was found (P = 0.0008). CONCLUSIONS: The presence of visible biofilm and low salivary activity of α amylase may be considered risk predictors for ECC.


Assuntos
Biofilmes/crescimento & desenvolvimento , Anidrases Carbônicas/metabolismo , Cárie Dentária/enzimologia , Incisivo , Saliva/enzimologia , alfa-Amilases/metabolismo , Criança , Pré-Escolar , Testes de Atividade de Cárie Dentária , Feminino , Humanos , Estudos Longitudinais , Masculino , Maxila , Medição de Risco , Estatística como Assunto
17.
Lasers Med Sci ; 31(3): 539-47, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26873502

RESUMO

This study evaluated if Carbon dioxide (CO2) (λ 10.6 µm) laser irradiation combined with acidulated phosphate fluoride gel application (APF gel) enhances "CaF2" uptake by demineralized enamel specimens (DES) and inhibits enamel lesion progression. Thus, two studies were conducted and DES were subjected to APF gel combined or not with CO2 laser irradiation (11.3 or 20.0 J/cm(2), 0.4 or 0.7 W) performed before, during, or after APF gel application. In study 1, 165 DES were allocated to 11 groups. Fluoride as "CaF2 like material" formed on enamel was determined in 100 DES (n = 10/group), and the surface morphologies of 50 specimens were evaluated by scanning electron microscopy (SEM) before and after "CaF2" extraction. In study 2, 165 DES (11 groups, n = 15), subjected to the same treatments as in study 1, were further subjected to a pH-cycling model to simulate a high cariogenic challenge. The progression of demineralization in DES was evaluated by cross-sectional microhardness and polarized light microscopy analyses. Laser at 11.3 J/cm(2) applied during APF gel application increased "CaF2" uptake on enamel surface. Laser irradiation and APF gel alone arrested the lesion progression compared with the control (p < 0.05). Areas of melting, fusion, and cracks were observed. CO2 laser irradiation, combined with a single APF application enhanced "CaF2" uptake on enamel surface and a synergistic effect was found. However, regarding the inhibition of caries lesion progression, no synergistic effect could be demonstrated. In conclusion, the results have shown that irradiation with specific laser parameters significantly enhanced CaF2 uptake by demineralized enamel and inhibited lesion progression.


Assuntos
Fluoreto de Cálcio/metabolismo , Cárie Dentária/terapia , Dente Molar/efeitos da radiação , Desmineralização do Dente/terapia , Fluoreto de Fosfato Acidulado/farmacologia , Fluoreto de Cálcio/farmacologia , Quimiorradioterapia , Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/metabolismo , Esmalte Dentário/patologia , Esmalte Dentário/efeitos da radiação , Géis , Humanos , Lasers de Gás/uso terapêutico , Microscopia Eletrônica de Varredura , Dente Molar/efeitos dos fármacos , Dente Molar/metabolismo , Dente Molar/patologia
18.
J Biomed Opt ; 20(10): 108003, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26502235

RESUMO

The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. Thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (RMGIC)], LEDTB [photodynamic antimicrobial chemotherapy (PACT) with light-emitting diode associated with toluidine blue solution and RMGIC], and LMB [PACT with laser associated with methylene blue solution and RMGIC]. The participants were submitted to initial clinical and radiographic examinations. Demographic features and biofilm, gingival, and DMFT/DMFS indexes were evaluated, in addition to clinical and radiographic followups at 6 and 12 months after treatments. Carious dentin was collected before and after each treatment, and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae, and total bacteria was established by quantitative polymerase chain reaction. No signs of pain or restoration failure were observed. All therapies were effective in reducing the number of microorganisms, except for S. sobrinus. No statistical differences were observed among the protocols used. All therapies may be considered as effective modern approaches to minimal intervention for the management of deep primary caries treatment.


Assuntos
Anti-Infecciosos/administração & dosagem , Infecções Bacterianas/diagnóstico , Infecções Bacterianas/tratamento farmacológico , Cárie Dentária/diagnóstico , Cárie Dentária/tratamento farmacológico , Fotoquimioterapia/métodos , Infecções Bacterianas/microbiologia , Criança , Pré-Escolar , Terapia Combinada/métodos , Cárie Dentária/microbiologia , Feminino , Humanos , Masculino , Fármacos Fotossensibilizantes/uso terapêutico , Dente Decíduo/efeitos dos fármacos , Dente Decíduo/microbiologia , Dente Decíduo/patologia , Resultado do Tratamento
19.
RGO (Porto Alegre) ; 62(1)jan.-mar. 2014. ilus
Artigo em Português | LILACS-Express | LILACS | ID: lil-712102

RESUMO

An alternative approach in the management of deep caries lesions is the indirect pulp treatment, without re-entry in the cavity. It has been shown that the complete removal of the carious dentin became unnecessary after the total tooth sealing, because the remaining microorganisms of the affected dentin would stop proliferating and/or die. This case report describes the management of a deep caries lesion of an 11-year-old girl with great coronary destruction in the left mandibular permanent first molar. Infected dentinal walls were excavated with manual instruments and the remainder affected dentin was filled with ionomer glass cement and composite resin. After 36 months of followup, no clinical and radiographic signs and symptoms of pulp damage were observed. In conclusion, the indirect pulp treatment was considered a good alternative for the treatment of deep caries lesions as it reduced the risk of pulp exposure by not reopening the cavity, saved the patient and the dentist clinical time and arrested the lesion progression with radiographic density increase and absence of any pain symptoms.


Um novo conceito cl?nico no tratamento de les?es de c?rie profunda ? o tratamento pulpar indireto sem reabertura da cavidade. Tem sido demonstrado que a remo??o completa da dentina cariada tornou-se desnecess?ria ap?s selamento total do dente, pois os microorganismosremanescentes da dentina afetada n?o se proliferariam ou morreriam. Este relato de caso descreve o tratamento de les?es de c?rie profunda de uma menina de 11 anos de idade com grande destrui??o coron?ria no primeiro molar permanente mandibular. As paredes dentin?rias infectadas foram removidas com instrumentos manuais e a dentina afetada remanescente foi preenchida com cimento de ion?mero de vidro e resina composta. Ap?s 36 meses de acompanhamento, n?o se observou nenhum sinal e sintoma cl?nico e radiogr?fico de dano pulpar. Em conclus?o, o tratamento pulpar indireto foi considerado uma boa alternativa para o tratamento de les?es de carie profunda, j? que reduziu o risco de exposi??o pulpar, devido a n?o reabertura da cavidade, economizou tempo cl?nico para o paciente e o dentista e paralisou a progress?o da les?o com aumento da densidade radiogr?fica e aus?ncia de qualquer sintomatologia dolorosa.

20.
Braz. dent. sci ; 17(1): 54-61, 2014. tab, ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: lil-728105

RESUMO

Objective: Although the effects of Er:YAG (erbium:yttrium aluminium garnet) laser on cavity preparation as well as on dentin bonding to composite have been described in the literature, the longevity of this bond is still unknown. So, this study evaluated the short-term microtensile bond strength to dentin samples after different protocols of surface treatment. Materials and Methods: 60 bovine incisors were cleaned, worn to expose a dentin area and subdivided into groups according to treatment conditions: surface treatment (no irradiation – control group; dentin irradiation with Er:YAG laser 250 mJ/4 Hz; 160 mJ/10 Hz), adhesive system (Clearfil SE Bond - Kuraray; Adper Single Bond 2 - 3M/ESPE), and storage time (24 h; 90 days). After adhesive procedures, a block of Z250 composite resin (3M/ESPE) was built-up on each tooth. The teeth were sectioned to obtain samples for the microtensile bond strength test. Half of the samples were tested 24 h after cutting, and the other half were stored in distilled water for 90 days before testing. Intergroup analysis was also performed considering the same variables using ANOVA for multiple comparisons with Tukey test with a significance level of 5%. Data showed weaker bond strength for groups previously treated with laser (p < 0.05) compared with control groups, and these were not influenced by adhesive system used, nor by storage period. Stereoscopic microscope observations showed that fractures occurred predominantly at the adhesive interface in the groups irradiated with the Er:YAG laser. Conclusion: Within the parameters and variables used in this study, the Er:YAG laser could not provide an additional improvement in dentin-resin bond strength, irrespective of the type of adhesive system used or the storage period evaluated.


Objetivo: Ainda que a ação do laser de Er:YAG no condicionamento e preparo do substrato dentinário, bem como na resistência de união à resina composta já tenha sido descrita na literatura, a longevidade da adesão decorrente deste processo ainda não está bem estabelecida. Material e Métodos: Neste estudo, ensaios de microtração foram realizados em palitos obtidos de 60 incisivos bovinos, subdivididos em 12 grupos constituídos pela combinação das variáveis: tratamento dentinário prévio com o laser de Er:YAG (250 mJ/ 4 Hz; 160 mJ / 10 Hz) e sem irradiação (grupo controle), sistema adesivo (Clearfil SE Bond /Kuraray; Adper Single Bond / 3M ESPE) e período de armazenagem (24 h; 90 dias). Os resultados mostraram menor resistência à microtração (com diferença estatisticamente significante p = 0,05) em relação aos grupos não tratados com o laser, não importando o sistema adesivo empregado, nem o período de armazenagem. A observação ao microscópio estereoscópico mostrou que as fraturas ocorreram predominantemente na interface adesiva para os grupos submetidos ao laser de Er:YAG. Conclusão: Portanto, a irradiação com o laser, nos parâmetros e variáveis utilizados e em comparação com os grupos controle, afetou negativamente a adesão à dentina, não havendo alteração relevante na longevidade da adesão para os períodos de armazenagem avaliados.


Assuntos
Animais , Bovinos , Resinas Compostas , Dentina , Lasers de Estado Sólido
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