Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 251
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-31640946

RESUMO

The anterolateral thigh (ALT) flap is becoming the flap of choice for reconstruction of soft tissue defects with primary donor site closure. The purpose of this report was to review the reconstruction of dorsal and palmar defects of the hand with ALT flaps from one donor site. From 2016 to 2018, seven patients with dorsal and palm defects of the hand were reconstructed with the ALT flaps from one donor site. The cutaneous perforators of the two paddles were from a common vascular supply in five cases. The Michigan Hand Questionnaire (MHQ) score of the function was 37.8 ±â€¯10.0 points, and pain score was 32.9 ±â€¯23.4 points. All of the donor sites healed well in one stage. The use of ALT flaps from one donor site is a good strategy for the reconstruction of dorsal and palm defects of the hand with minimal donor site morbidity and a favorable outcome.

2.
ACS Nano ; 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31651153

RESUMO

Filamentous microorganisms traveling in aerosol particles display enhanced deposition and retention in the lungs. Inspired by this shape-related biological effect, we report here on the use of supramolecular filaments as potential inhalable drug carriers within aerosols via jet nebulization. We found that the peptide design and supramolecular stability play a crucial role in the interfacial stability and aerosolization properties of the supramolecular filaments. Monomeric units with a positively charged C-terminus produced filaments with reduced aerosol stability, promoting morphological changes after nebulization. Conversely, having a neutral or negatively charged terminus yielded filaments with enhanced stability, where supramolecular integrity is maintained with only reduced length. Our results suggest that molecular enrichment at the air-liquid interface during nebulization is the primary factor to deplete the monomeric peptide amphiphiles in solution, accounting for the observed morphological disruption/transitions. Importantly, encapsulation of drugs and dyes within filaments notably stabilize their supramolecular structure during nebulization, and the loaded filaments exhibit a linear release profile from a nebulizer device. We envision the use of this supramolecular carrier system as an effective platform for the inhalation-based treatment of many lung diseases.

3.
J Am Chem Soc ; 141(43): 17107-17111, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31614088

RESUMO

Nanostructured supramolecular polymers (SPs) are filamentous assemblies possessing a high degree of internal order and have important uses in regenerative medicine, drug delivery, and soft matter electronics. Despite recent advances in functional SPs, a challenging topic is the development of robust assembly protocols enabling the incorporation of various functional units without altering its supramolecular architecture. We report here the robust tubular assembly of camptothecin (CPT) analogues into functional SPs. Covalent linkage of two CPT moieties to various short hydrophilic segments (e.g., nonionic, cationic, anionic, and zwitterionic) leads to a class of CPT analogues that self-assemble in water into tubular SPs. Systemic administration of nonionic SPs effectively suppresses tumor growth. Furthermore, these tubular SPs act as universal dispersing agents in water for low-molecular-weight hydrophobes.

4.
Clinics (Sao Paulo) ; 74: e1077, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596338

RESUMO

OBJECTIVES: This study investigated whether tissue Doppler imaging parameters, especially the peak systolic velocity of the left ventricular lead-implanted segment (Ss), affect cardiac resynchronization therapy response. METHODS: In this case-control study, 110 enrolled patients were divided into cases (responder group, n=65) and controls (nonresponder group, n=45) based on whether their left ventricular end-systolic volume was reduced by ≥15% at 6 months after surgery. Preoperative clinical and echocardiographic data were collected. Multivariate logistic regression models were used to analyze the factors affecting the response to cardiac resynchronization therapy, and receiver operating characteristic curves were plotted to evaluate their diagnostic values. RESULTS: The proportion of patients with left bundle branch block in the case group was higher than that in the control group. The control group showed a higher left atrial volume index, E/A ratio and E/Em ratio but lower Ss than that of the case group. A multivariate regression analysis showed that left bundle branch block, Ss, and an E/Em ratio>14 were independent risk factors affecting the response to cardiac resynchronization therapy. Ss=4.1 cm/s was the best diagnostic threshold according to the receiver operating characteristic curve. CONCLUSIONS: Ss is an important factor affecting the response to cardiac resynchronization therapy. Patients with heart failure associated with Ss<4.1 cm/s have a higher risk of nonresponse.

5.
Sci Total Environ ; 692: 741-755, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31539982

RESUMO

Conventional nitrification/denitrification process is gradually being replaced with partial-nitritation/anammox (PN/A) processes due to its installation and running cost. However, high ammonia-oxidizing bacteria (AOB) and anaerobic ammonia-oxidizing (anammox) bacteria activity as well as optimum out-selection of nitrite-oxidizing bacteria (NOB) are necessary to achieving efficient PN/A process. Consequently, to enhance PN process via nitrifying bacteria enrichment/out-selection within psychrophilic environment, a novel pH-DO (dissolved oxygen) control strategy was proposed and the response of PN, kinetics, AOB enrichment, and NOB out-selection efficiency was investigated during start-up and long-term operation. With DO of 0.7 mg/L and pH of 7.5-7.9, quick start-up of the PN process was established within 34d as NO2--N accumulation ratio (NAR) reached 90.08 ±â€¯1.4%. Again, when NLR was elevated to 0.8 kg/m3·d (400mgNH4+-N/L), DO curtailed to 0.2 mg/L, pH maintained at 7.7 and free ammonium at 6.5 mg/L, NAR and NH4+-N removal rate could still reach 97.04 ±â€¯2.4% and 97.84 ±â€¯1.5%, respectively. After optimum control factors had been established, real nitrogen-rich-mine-wastewater was fed (DO, 0.2 mg/L, pH, 8.9, and free ammonia, 6.5 mg/L) and NAR and NH4+-N removal rate reached was 97.33 ±â€¯0.5% and 97.76 ±â€¯1.1%, respectively. Estimated kinetic parameters including maximum degradation rate (Vmax = 1.58/d), half-rate constant (Km = 33.8 mg/L), and inhibition constant (Ki = 201.6 mg/L) suggested that inhibition on NH4+-N oxidation was most feasible at higher concentration of NH4+-N. To elucidate biological mechanisms, 16S rRNA high-throughput revealed that AOB (Nitrosomonas) enrichment had increased from 0.08% to 49% whereas NOB (Nitrospira) abundance reduced from 1% to 0.034%, indicating pH-DO control efficiently enriched AOB and out-selected NOB. Conversely, when influent NH4+-N was curtailed to about 200 mg/L and free ammonia concentration maintained at 6.5 mg/L, the population of AOB was observably reduced by 6% within a period of 14 days, indicating control strategies including pH-DO control and substrate availability were the key factors which substantially influenced and promoted the activities and growth of AOBs in the present SBR.

6.
Chemistry ; 2019 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-31495994

RESUMO

Sodium-ion batteries have attracted interest as an alternative to lithium-ion batteries because of the abundance and cost effectiveness of sodium. However, suitable anode materials with high-rate and stable cycling performance are still needed to promote their practical application. Herein, three-dimensional Na2 Ti3 O7 nanowire arrays with enriched surface vacancies endowed by phosphorus doping are reported. As anodes for sodium-ion batteries, they deliver a high specific capacity of 290 mA h g-1 at 0.2 C, good rate capability (50 mA h g-1 at 20 C), and stable cycling capability (98 % capacity retention over 3100 cycles at 20 C). The superior electrochemical performance is attributed to the synergistic effects of the nanowire arrays and phosphorus doping. The rational structure can provide convenient channels to facilitate ion/electron transport and improve the capacitive contributions. Moreover, the phosphorus-doping-induced surface vacancies not only provide more active sites but also improve the intrinsic electrical conductivity of Na2 Ti3 O7 , which will enable electrode materials with excellent sodium storage performance. This work may provide an effective strategy for the synthesis of other anode materials with fast electrochemical reaction kinetics and good sodium storage performance.

7.
Proc Natl Acad Sci U S A ; 116(40): 19799-19801, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31527270

RESUMO

The cytoskeleton provides structural integrity to cells and serves as a key component in mechanotransduction. Tensins are thought to provide a force-bearing linkage between integrins and the actin cytoskeleton; yet, direct evidence of tensin's role in mechanotransduction is lacking. We here report that local force application to epithelial cells using a micrometer-sized needle leads to rapid accumulation of cten (tensin 4), but not tensin 1, along a fibrous intracellular network. Surprisingly, cten-positive fibers are not actin fibers; instead, these fibers are keratin intermediate filaments. The dissociation of cten from tension-free keratin fibers depends on the duration of cell stretch, demonstrating that the external force favors maturation of cten-keratin network interactions over time and that keratin fibers retain remarkable structural memory of a cell's force-bearing state. These results establish the keratin network as an integral part of force-sensing elements recruiting distinct proteins like cten and suggest the existence of a mechanotransduction pathway via keratin network.

8.
Cardiology ; 144(1-2): 18-26, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31476753

RESUMO

BACKGROUND: Heart failure may induce atrial dyssynchrony. We aim to investigate whether preimplantation left atrial (LA) dyssynchrony could predict newly detected atrial high-rate episodes (AHRE) after receiving cardiac resynchronization therapy defibrillator (CRT-D). METHODS: We conducted a retrospective analysis of consecutive patients who received CRT-D for standard indications and without a history of atrial fibrillation. The standard deviation of the time-to-peak strain in each LA segment during ventricular systole (SDs) and late diastole (SDa) were calculated to quantify LA dyssynchrony using two-dimensional speckle tracking echocardiography before device implantation. Patients were divided into the AHRE group and the AHRE-free group, depending on the presence of AHRE during device interrogation. RESULTS: Thirty-one patients (28%) had newly detected AHRE during a mean follow-up of 21 ± 9 months. Patients in the AHRE group had higher SDs (8.2 ± 2.6% vs. 6.3 ± 2.3%, p < 0.001) and SDa (5.4 ± 1.8% vs. 4.1 ± 1.4%, p < 0.001) values before implantation than patients in the AHRE-free group. In the multivariate logistic analysis, both SDs (OR 1.325, 95% CI: 1.074-1.636, p =0.009) and SDa (OR: 1.499, 95% CI: 1.071-2.098, p= 0.018) were independent predictors of newly detected AHRE. At a cutoff value of 7.4% for SDs and 5.3% for SDa, the Kaplan-Meier survival analysis showed that patients with higher SDs and SDa had significantly increased risks of newly detected AHRE after receiving CRT-D. CONCLUSIONS: Dyssynchronous LA lengthening and contraction could assist in the prediction of newly detected AHRE in patients with CRT-D.

9.
Cancer Med ; 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31489986

RESUMO

BACKGROUND: The aim of this study was to investigate the potential prognostic value of Kinesin-4 family genes mRNA expression in early-stage pancreatic ductal adenocarcinoma (PDAC) patients after pancreaticoduodenectomy. METHODS: Kaplan-Meier survival analysis method with log-rank test and Cox proportional hazards regression analysis were performed to figure out the association between Kinesin-4 family genes expression and PDAC patients overall survival time. Joint-effect survival analysis and stratified survival analysis were carried out to assess the prognosis prediction value of prognosis-related gene. Nomogram was constructed for the individualized prognosis prediction. In addition, we had used the gene set enrichment analysis and genome-wide co-expression analysis to further explore the potential mechanism. RESULTS: KIF21A expression level was significantly associated with PDAC patient clinical prognosis outcome and patient with a high expression of KIF21A would have a shorter overall survival time. The prognosis prediction significance of KIF21A was well validated by the joint-effect survival analysis, stratified survival analysis, and nomogram. Meanwhile, the gene set enrichment analysis and genome-wide co-expression analysis revealed that KIF21A might involve in DNA damage and repair, transcription and translation process, post-translation protein modification, cell cycle, carcinogensis genes and pathways. CONCLUSIONS: Our current research demonstrated that KIF21A could serve as a potential prognostic biomarker for patient with early-stage PDAC after pancreaticoduodenectomy.

10.
Int J Mol Med ; 44(5): 1932-1942, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31545484

RESUMO

Isosteviol sodium (STVNa), which is a derivate of the natural sweet­tasting glycoside stevioside, has recently been developed and it has been determined that this compound exhibits neuro­ and cardio­protective properties. In the current study, whether STVNa interferes with the development of cardiac hypertrophy, which is induced by isoprenaline (Iso), was investigated in an experimental rat model. Rats were treated with a vehicle (0.9% NaCl; control), isoprenaline (Iso; 5 mg/kg) or Iso (5 mg/kg) with STVNa (4 mg/kg; Iso + STVNa). Cardiomyocytes were isolated using enzymatic dissociation and were treated with 5 µM Iso for 24 h and co­treated with 5 µM STVNa. Brain natriuretic peptide (BNP) mRNA expression was determined using PCR analysis. Cell surface area, intracellular reactive oxygen species (ROS), mitochondrial transmembrane potential (ΔΨm), cytoplasmic Ca2+ and Ca2+ and contractile function were examined using a laser scanning confocal microscope. The current study demonstrated that STVNa inhibited Iso­induced cardiac hypertrophy by inhibiting cardiomyocyte size. STVNa significantly reduced cell surface area and decreased BNP mRNA expression in ventricular cardiomyocyte Iso­induced hypertrophy. STVNa was also revealed to restore ΔΨm and reduce ROS generation and intracellular Ca2+ concentration when compared with the Iso­treated group. Additionally, STVNa preserved Ca2+ transients in hypertrophic cardiomyocytes. In conclusion, the present study demonstrated that STVNa protects against Iso­induced myocardial hypertrophy by reducing oxidative stress, restoring ΔΨm and maintaining Ca2+ homeostasis.

11.
Oncol Rep ; 42(5): 1856-1868, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31432181

RESUMO

The present study aimed to determine the clinical significance and potential molecular mechanisms of C­C motif chemokine receptor (CCR) genes in patients with early­stage pancreatic ductal adenocarcinoma (PDAC). The transcriptomic, survival and clinical data of 112 patients with early­stage PDAC who underwent pancreaticoduodenectomy were obtained from The Cancer Genome Atlas. The prognostic values of the CCR genes involved in early­stage PDAC were evaluated using Kaplan­Meier analysis and the multivariate Cox proportional risk regression model, and the potential molecular mechanisms were determined using bioinformatics tools. The identified CCRs closely interacted with each other at both the gene and protein levels. High expression levels of CCR5 [adjusted P=0.012; adjusted hazard ration (HR)=0.478, 95% confidence interval (CI)=0.269­0.852], CCR6 (adjusted P=0.026; adjusted HR=0.527, 95% CI=0.299­0.927) and CCR9 (adjusted P=0.001; adjusted HR=0.374, 95% CI=0.209­0.670) were significantly associated with longer overall survival times in patients with early­stage PDAC. The contribution of CCR5, CCR6 and CCR9 to the outcome of early­stage PDAC was also demonstrated. Combined survival analysis of CCR5, CCR6 and CCR9 suggested that patients with high expression levels of these CCRs exhibited the most favorable outcomes. A prognostic signature was constructed in terms of the expression level of CC5, CCR6 and CCR9, and time­dependent receiver operating characteristic curves indicated that this signature was able to effectively predict the outcome of patients with early­stage PDAC. The potential molecular mechanisms of CCR5, CC6 and CCR9 in PDAC include its intersection of the P53, nuclear factor (NF)­κB, generic transcription, mitogen­activated protein kinase and STAT signaling pathways. Collectively, this highlights that CCR5, CCR6 and CCR9 are potential prognostic biomarkers for early­stage PDAC.

12.
Chem Commun (Camb) ; 55(67): 9923-9926, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31368462

RESUMO

A series of gold-catalysed intramolecular anti-Markovnikov hydroamination-initiated azidation, allylation and heteroarylation reactions of chiral homopropargyl sulfonamides have been developed. Various enantioenriched 2,5-disubstituted pyrrolidines are obtained in moderate to excellent yields with excellent enantioselectivities and generally high diastereoselectivities.

13.
Int Immunopharmacol ; 75: 105787, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31401382

RESUMO

Estrogen has been reported to inhibit neutrophil infiltration related inflammation and suppress neutrophils migration in vitro, but the underlying mechanism is not fully understood. By using HL-60 differentiated neutrophil-like cells (dHL-60) and human neutrophils, we examined the effect of 17-ß estradiol (E2) on cell migration and superoxide production in response to chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) and explored the mechanisms involved. We found that fMLP significantly induced dHL-60 cell and neutrophil migration and superoxide production, which was inhibited by ERK inhibitor PD98059. E2 significantly inhibited fMLP-induced dHL-60 cell and neutrophil migration and superoxide production at both physiological and pharmacological concentrations. Mechanistic studies showed that pretreatment of these cells with E2 rapidly elevated the protein level of mitogen-activated protein kinase phosphatase 2 (MKP-2) and inhibited fMLP-induced ERK phosphorylation. Pretreatment of these cells with estrogen receptor (ER) antagonist ICI 182780 reversed the inhibition of fMP-induced cell migration and superoxide production, and the induction of MKP-2 expression and the suppression of fMP-induced ERK phosphorylation by E2. However, pretreatment of cells with G-protein coupled ER antagonist G15 had no such effect. Collectively, these results demonstrate that fMLP stimulates neutrophil chemotaxis and superoxide production through activating ERK, and indicate that ER-mediated upregulation of MKP-2 may dephosphorylate ERK and contribute to the inhibitory effect of E2 on neutrophil activation by fMLP. Our study reveals new mechanisms involved in the anti-inflammatory activity of estrogen.

14.
Oncol Rep ; 42(3): 895-910, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31322232

RESUMO

The aim of the present study was to investigate the diagnostic and prognostic value of Wingless­type MMTV integration site (WNT) gene family expression in patients with hepatitis B virus (HBV)­related hepatocellular carcinoma (HCC). The clinical data of the patients and gene expression levels were downloaded from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Receiver operating characteristic curve analysis was used to investigate the diagnostic value of WNT genes. Cox proportional hazard regression analysis and Kaplan­Meier survival analysis were performed to evaluate the association of WNT gene expression level with overall survival (OS) and recurrence­free survival (RFS). A nomogram was constructed for the prediction of prognosis. Hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated. Diagnostic receiver operating characteristic curve analysis suggested that WNT2 had a high diagnostic value, with an area under the curve (AUC) of >0.800 (P<0.0001, AUC=0.810, 95% CI: 0.767­0.852). Survival analysis indicated that the expression level of WNT1 was significantly associated with OS and RFS (adjusted P=0.033, adjusted HR=0.607, 95% CI: 0.384­0.960; and adjusted P=0.007, adjusted HR=0.592, 95% CI: 0.404­0.868, respectively). In the TCGA validation cohort, we also observed that WNT2 was significantly differentially expressed between HCC tissues and adjacent non­tumor tissues, and WNT1 was associated with both the OS and RFS of HCC. Therefore, through the GSE14520 HBV­related HCC cohort we concluded that WNT2 may serve as a diagnostic biomarker and WNT1 may serve as a prognostic biomarker. These results may also be extended to TCGA HCC verification cohort.

15.
Medicine (Baltimore) ; 98(29): e16377, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31335687

RESUMO

RATIONALE: Small intestine stromal tumors (SISTs) are a type of gastrointestinal stromal tumor (GIST) that has an insidious onset. Natural orifice specimen extraction (NOSE) surgery has been gradually developed for the treatment of colorectal, stomach, small intestine, hepatobiliary, and gynecological tumors because of its safety and feasibility. This case study explored the possibility of applying the NOSE method for the treatment of SIST. PATIENT CONCERNS: A 59-year-old male patient was admitted to the hospital after having an irregular abdominal mass for >1 month that was detected by a medical examination. Thoracic and abdominopelvic enhanced computer tomography revealed irregular masses on the left side of the abdominal cavity. DIAGNOSIS: Sist. INTERVENTIONS: Nose (laparoscopic resection of intestinal stromal tumors with transrectal extract specimen and no abdominal auxiliary incision) surgery was performed. OUTCOMES: The patient underwent operation successfully and recuperates well with no complications. LESSONS: Nose surgery is minimally invasive, results in patient recuperation with no complications, and is considered to be feasible for SIST treatment.


Assuntos
Colectomia/métodos , Tumores do Estroma Gastrointestinal , Neoplasias Intestinais , Laparoscopia/métodos , Cirurgia Endoscópica por Orifício Natural/métodos , Tumores do Estroma Gastrointestinal/patologia , Tumores do Estroma Gastrointestinal/fisiopatologia , Tumores do Estroma Gastrointestinal/cirurgia , Humanos , Neoplasias Intestinais/patologia , Neoplasias Intestinais/fisiopatologia , Neoplasias Intestinais/cirurgia , Intestinos/diagnóstico por imagem , Intestinos/patologia , Intestinos/cirurgia , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento
17.
Ultrason Sonochem ; 57: 48-56, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31208618

RESUMO

This study uses a novel fabrication method of thin metal coatings by Ni electroplating combining ultrasonic agitation with supercritical CO2 (US-SC-CO2) mixed into conventional electrolyte. Coatings were also produced by the conventional and regular SC-CO2 electroplating methods for comparison. The characteristics obtained from the three fabrication methods such as surface morphology, hardness, roughness; crystallographic orientation, grain size; wear and corrosion resistance were all individually analyzed. Results show that plating quality achieved by US-SC-CO2 method is superior to that of regular SC-CO2 and conventional methods. With US-SC-CO2 process we achieved smoother and more compact surface morphologies, smaller grain size, lower surface roughness and higher microhardness, which also suggests good wear resistance. From XRD analysis we observed changes in preferred orientation due to application of the various methods. From the results of US-SC-CO2 electroplating and the operating mechanism of ultrasonic agitation we can confirm that this new type of ultrasonic agitation can indeed replace the role of surfactants to enhance coating aspect and properties, reducing their influence over the deposited metal coating, associated costs, and waste. In H2SO4 solution, the nickel coating fabricated by US-SC-CO2 method displayed the best polarization resistance among the three processes. More detailed experimental results and in depth discussion are presented in this paper.

18.
Int Immunopharmacol ; 73: 581-589, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31234092

RESUMO

Inhaled terbutaline is commercially available ß2-agonist which consists of equivalent amount of R- and S-enantiomer. In this study, we aimed to investigate the effects of single enantiomers of terbutaline and its racemate in an ovalbumin (OVA)-induced mouse model of asthma via. seven days inhalation and the potential mechanisms involved. In a standard experimental asthma model, BALB/c mice were sensitized and challenged with OVA. R-terbutaline (R-ter), S-terbutaline (S-ter) or racemic terbutaline (rac-ter) was given via. nose-only inhalation for one week. Airway responsiveness to methacholine was measured by the plethysmography in conscious mice. Eosinophils counts in blood and bronchoalveolar (BAL) fluid were determined. The OVA-sIgE in plasma and inflammatory cytokines and mediators in BAL fluid or lung tissue were analyzed by ELISA, qRT-PCR or western blotting. Airway inflammation and remodeling were evaluated with hematoxylin and eosin (HE), periodic acid-Schiff (PAS), and Masson staining. Drug distribution and deposition after inhalation were determined by LC-MS/MS. Our data showed that R-ter efficiently ameliorated asthma responses, including airway hyperresponsiveness, eosinophils influx and IL-5 in BALF, plasma OVA-sIgE and significantly reduced pulmonary inflammation, peribronchial smooth muscle layer thickness, goblet cell hyperplasia, and deposition of collagen fibers, as well as downregulation of p38 MAPK phosphorylation and NF-κB expression. Racemic mixture exhibited diminished effects while S-ter enhanced airway responsiveness to methacholine and exerted pro-asthmatic effects.

19.
J Am Chem Soc ; 141(30): 11997-12004, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31251590

RESUMO

Spontaneous association above a threshold concentration is a hallmark of supramolecular polymerization, in which monomeric units self-assemble into polymeric aggregates through noncovalent interactions. This self-initiated supramolecular process differs from the conventional covalent chain-growth polymerization in that the latter often involves the use of a different chemical entity as an initiator to trigger/control the polymerization process. We report here the use of a small molecule hydrophobe, paclitaxel (PTX), as an effective promoter to induce the supramolecular polymerization of a peptide-paclitaxel conjugate, Spheropax (Spax). We found that Spax monomers alone in water self-assemble into spherical micelles of approximately 6.5 nm in diameter but, in the presence of free PTX, undergo a supramolecular polymerization process to form filamentous assemblies of several micrometers in length. Increasing the ratio of promoter to monomer (PTX/Spax) induces Spax's directional polymerization and expedites its kinetic process. We believe these findings provide important insight into the initiator-controlled supramolecular polymerization process.

20.
Asian J Androl ; 21(5): 433-437, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31134920

RESUMO

Prostate cancer is the most common malignancy in the reproductive system of older males. Androgen deprivation therapy (ADT) is an important treatment for prostate cancer patients. However, almost all prostate cancer patients unavoidably progress to the castration-resistant stage after ADT treatment. Recent studies have shown that tumor-associated immune cells play major roles in the initiation, progression, and metastasis of prostate cancer. Various phenotypes of tumor-associated immune cells have tumor-promoting or antitumor functions mediated by interacting with tumor cells. Here, we review the current knowledge of tumor-associated immune cells in prostate cancer.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA