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1.
Carbohydr Polym ; 229: 115390, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31826456

RESUMO

Functionalized nano-starch particles, designed for the adsorption of heavy metals in wastewater, were prepared by a nano-processing, a halogenated grafting modification, a grafting copolymerization of surface-initiated atom transfer radical polymerization (SI-ATRP) and a quaternized modification of native corn starch. The influence of the synthesis process variables, such as the hydrolysis time, the concentration of monomer, the molar ratio of copper bromide (CuBr) to 2, 2'-bipyridine (bpy) and the graft copolymerization temperature on the properties of the products were studied. The morphology, molecular structure, crystalline structure of the functionalized nano-starch and its derivatives were characterized by Fourier transform-infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (1H-NMR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and transmission electron microscope (TEM). The functionalized nano-starch showed strong adsorption for chromate and could be used as an effective wastewater treatment agent. Its adsorption capability could be almost totally regenerated by an easy process.

2.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(6): 1749-1753, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31839033

RESUMO

OBJECTIVE: To investigate the effect of BAX gene deletion on the sensitivity of BCR-ABL-induced B-ALL cells of mice to imatinib and the related mechanism. METHODS: The target gene-knock out (BAX-/-) mice were used as bone marrow cell donors; the wild type bone marrow cells(B6BM) and BAX-/- bone marrow cells(B6BM-BAX-/-) of mice were transfected by using reverse transcription virus, then the BCR-ABL transfected B6BM cells and B6BM-BAX-/- cells were treated with imatinib at different concentration (0,0.5, 1.0 and 2.0 µmol/L) for 48 hours. The number of viable cells was detected by trypan blue, the flow cytometry was used to detect the cell apoptosis, the Western blot was used to detect the changes of BAX, Caspase expression. RESULTS: In BCR-ABL transfected bone marrow cells treated with imatinib, the numbers of viable cells of BAX deletion group was significantly higher than that of wild type groups with statristcal difference(P<0.05), and effect- and dose-dependency(r=-0.9533 for BAX deletion group, and r=-0.9812 for wild type group). The flow cytometry showed that the cell apoptosis in BAX deletion group signifincantly decreased, compared with wild type group(P<0.05). The Western blot showed that the expression of apoptotic protein Caspase 3 in BAX deletion group was significantly higher than that in wild type group(P<0.05). CONCLUSION: BAX deletion can reduce the sensitivity of BCR-ABL-induced B-ALL cells to imatinib.


Assuntos
Leucemia-Linfoma Linfoblástico de Células Precursoras B/genética , Animais , Apoptose , Resistencia a Medicamentos Antineoplásicos , Proteínas de Fusão bcr-abl , Deleção de Genes , Mesilato de Imatinib , Camundongos , Piperazinas , Proteína X Associada a bcl-2
3.
Artigo em Inglês | MEDLINE | ID: mdl-31769063

RESUMO

BACKGROUND: Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment. No safe and effective radiation countermeasure has been approved for clinical therapy. In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury. MATERIALS AND METHODS: C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy. The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated. RESULTS: Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation. Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1). PC pretreatment significantly ameliorated radiation-induced intestinal injury. PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation. Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated. CONCLUSION: PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.

4.
Future Oncol ; 15(35): 4105-4118, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31746639

RESUMO

Aim: To systematically review the prophylactic and therapeutic interventions for reducing the incidence or severity of intestinal symptoms among cancer patients receiving radiotherapy. Materials & methods: A literature search was conducted in the PubMed database using various search terms, including 'radiation enteritis', 'radiation enteropathy', 'radiation-induced intestinal disease', 'radiation-induced intestinal damage' and 'radiation mucositis'. The search was limited to in vivo studies, clinical trials and meta-analyses published in English with no limitation on publication date. Other relevant literature was identified based on the reference lists of selected studies. Results: The pathogenesis of acute and chronic radiation-induced intestinal damage as well as the prevention and treatment approaches were reviewed. Conclusion: There is inadequate evidence to strongly support the use of a particular strategy to reduce radiation-induced intestinal damage. More high-quality randomized controlled trials are required for interventions with limited evidence suggestive of potential benefits.

5.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(5): 1469-1475, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31607300

RESUMO

OBJECTIVE: To investigate the effect of LNK gene silencing and overexpression on the expression of STAT3 gene in human monocytic leukemia cells (THP-1). METHODS: THP-1 cells were cultured, and the lentivirus was used as a vector to silence and overexpres the LNK gene stably. After transfection for 72 hours, the GFP expression levels were observed by inverted fluorescence microscopy. The lentiviral transfection efficiencies were detected by flow cytometry. The effects of LNK silencing and overexpression were confirmed, and the expression of STAT3 mRNA was detected by RT-PCR. The protein levels of LNK and STAT3 were detected by Western blotting. RESULTS: The GFP expression level of THP-1 cells reached more than 85% after transfection with lentivirus for 72 hours, and the transfection efficiency of cells was above 99%. mRNA expressions levels of LNK and STAT3 in LNK silencing group were signifycantly lower than those in control group, while LNK and STAT3 mRNA levels in the LNK overexpression group was significantly higher than those in control group (P<0.05). The protein expression levels of LNK and STAT3 in LNK silencing group were significantly lower than those in control group, while that in LNK overexpression group was significantly higher than that in control group (P<0.05). CONCLUSION: The THP-1 cell line with LNK gene silencing and overexpression has been successfully established. The LNK gene silencing resulted in decrease of STAT3 expression; LNK gene overexpression and leads to inereases of STAT3 expression indicating that LNK participates in the regulation of STAT3.


Assuntos
Fator de Transcrição STAT3/metabolismo , Linhagem Celular Tumoral , Inativação Gênica , Vetores Genéticos , Humanos , Peptídeos e Proteínas de Sinalização Intracelular , Lentivirus , Proteínas , RNA Interferente Pequeno , Células THP-1 , Transfecção
6.
Arch Med Sci ; 15(5): 1294-1300, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31572476

RESUMO

Introduction: Controlled ovarian hyperstimulation (COH) is essential for artificial reproduction technology (ART). This study aimed to evaluate the effects of a mild starting dosage of r-FSH ovarian stimulation after the modified prolonged GnRH-a down-regulation protocol for COH on the clinical outcomes in normal ovarian responders undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). Material and methods: In the retrospective study, the patients were separated into two groups according to the starting dosage of r-FSH: a mild dosage group (75 IU ≤ r-FSH < 150 IU, n = 858) and a conventional dosage group (150 IU ≤ r-FSH ≤ 225 IU, n = 535). Data were collected from clinical records. The baseline characteristics and clinical outcomes were compared between the two groups. Results: Although the duration of r-FSH treatment was a little longer in the mild dosage group, the total r-FSH dosage and the cost of ovarian stimulation were significantly lower than those in the conventional dosage group. Furthermore, compared to the conventional dosage group, the number of retrieved oocytes was also lower in the mild dosage group, whereas the rates of two pronuclei (2PN) fertilized oocytes and good-quality embryos were remarkable higher. The implantation rate, clinical pregnancy rate and live birth rate were significantly higher in the mild dosage group. There was no difference in early miscarriages rate, incidence of moderate and severe ovarian hyper-stimulation syndrome (OHSS) or incidence of ectopic pregnancy between the two groups. Conclusions: The modified prolonged GnRH-a pituitary down-regulation regimen combined with mild r-FSH starting dosage improved IVF/ICSI outcomes and reduced the financial cost in normal ovarian responders.

7.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1013-1019, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418350

RESUMO

OBJECTIVE: To investigate the effect of silencing LNK gene on the expression of EPO and EPOR in acute myeloid leukemia cells (THP-1). METHODS: THP-1 cells were cultured. The lentivirus was used as a vector to silence the LNK gene stably. After 72 hours of infection, GFP expression level was detected by the fluorescent inverted microscopy. The lentiviral Infection efficiencies were monitored by flow cytometry. The LNK silencing effect was confirmed. The mRNA expressions of EPO and EPOR were detected by RT-PCR. The protein levels of LNK, EPO and EPOR were detected by Western blot. RESULTS: At the time-point of 72 hours after lentivirus infection, the expression level of GFP was above 85% detected by fluorescent inverted microscopy. The infection efficiency was above 99% by flow cytometry. mRNA expressions of LNK, EPO and EPOR in LNK silencing group were signifycantly lower than those in control group (P<0.05). The protein levels of LNK, EPO and EPOR in LNK silencing group were significantly lower than those in the control group (P<0.05). CONCLUSION: THP-1 cell line of LNK gene silencing has been successfully established,the LNK gene has been silenced, the expression of EPO and EPOR decrease, indicating that LNK may participate in the regulation of EPO and EPOR.


Assuntos
Proteínas/genética , Western Blotting , Eritropoetina , Inativação Gênica , Humanos , Peptídeos e Proteínas de Sinalização Intracelular , Receptores da Eritropoetina , Células THP-1
8.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1026-1032, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418352

RESUMO

OBJECTIVE: To investigate the expression of erythropoietin (EPO) and erythropoietin receptor (EPOR) in patients with acute leukemia (AL) and its clinical significance. METHODS: The levels of EPO and EPOR in plasma were determined by ELISA kit. mRNA expression levels of EPO and EPOR were determined by RT-RCR. The protein expression levels of EPO and EPOR were detected by Western blot. RESULTS: The EPO protein levels in marrow plasma of ALL and AML group were significantly higher than those in the control group (P<0.05), EPOR protein levels in ALL and AML group were significantly lower than those in the control group (P<0.05). The mRNA levels of EPO and EPOR in ALL and AML groups were significantly higher than those in the control group (P<0.05). The mRNA levels of EPO and EPOR in the high risk ALL and AML groups were significantly higher than those in the medium, low risk group and the control group (P<0.05). The protein expression levels of EPO and EPOR in ALL and AML groups were significantly higher than that in control group (P<0.05). The mRNA levels of EPO and EPOR in ALL and AML groups did not correlate with hemoglobin level and erythrocyte count (P>0.05). CONCLUSION: The expressions of EPO and EPOR is higher in ALL and AML patients. The expression levels of EPO and EPOR relate with the risk of ALL and AML. High risk patients have higher expression levels of EPO and EPOR, however, the expression levels of EPO and EPOR do not correlate with hemoglobin level and erythrocyte counting.


Assuntos
Leucemia Mieloide Aguda , Medula Óssea , Eritropoetina , Expressão Gênica , Humanos , Receptores da Eritropoetina
9.
Adv Sci (Weinh) ; 6(16): 1900162, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31453056

RESUMO

The inferior tolerance with reversible accommodation of large-sized Na+ ion in electrode materials has plagued the adaptability of sodium-ion chemistry. The sluggish diffusion kinetics of Na+ also baffles the desirability. Herein, a carbon fiber supported binder-free electrode consisting of bismuth and carbon composite is designed. Well-confined bismuth nanodots are synthesized by replacing cobalt in the metal-organic frameworks (MOF)-derived, nitrogen-doped carbon arrays, which are demonstrated with remarkable reversibility during sodiation and desodiation. Cobalt species in the pristine MOF catalyze the graphitization around organic components in calcination, generating a highly conductive network in which the bismuth is to be embedded. The uniformly dispersed bismuth nanodots provide plenty boundaries and abundant active sites in the carbon arrays, where fast sodium storage kinetics are realized to contribute extra capacity and excellent rate performance.

10.
Clin Exp Med ; 19(4): 417-426, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31313081

RESUMO

Cancer research has advanced throughout the years with respect to the personalization of the treatments and to targeting cancer-related molecular signatures on different organs. Still, the adverse events of the treatments such as radiotherapy are of high concern as they may increase the mortality rate due to their severity. With the improved efficiency of cancer treatments, patient survival has been increasing. Consequently, the number of patients with adverse effects from radiotherapy is also expected to increase in the forthcoming years. Therefore, approaches for personalized treatments include the elimination of adverse events and decreasing the toxicity in healthy tissues while increasing the efficiency of cancer cytotoxicity. In this context, this paper aims to discuss the recent advances in the field of thorax irradiation therapy and its related toxicities leading to radiation pneumonitis in cancer patients. Molecular mechanisms involved in the radiation-induced lung injury and approaches used to overcome this lung injury are discussed. The discourse covers approaches such as therapeutic administration of natural products, current and prospective radioprotective drugs, and applications of mesenchymal stem cells for radiation-induced lung injury.

11.
N Engl J Med ; 381(12): 1124-1135, 2019 09 19.
Artigo em Inglês | MEDLINE | ID: mdl-31150573

RESUMO

BACKGROUND: Platinum-based concurrent chemoradiotherapy is the standard of care for patients with locoregionally advanced nasopharyngeal carcinoma. Additional gemcitabine and cisplatin induction chemotherapy has shown promising efficacy in phase 2 trials. METHODS: In a parallel-group, multicenter, randomized, controlled, phase 3 trial, we compared gemcitabine and cisplatin as induction chemotherapy plus concurrent chemoradiotherapy with concurrent chemoradiotherapy alone. Patients with locoregionally advanced nasopharyngeal carcinoma were randomly assigned in a 1:1 ratio to receive gemcitabine (at a dose of 1 g per square meter of body-surface area on days 1 and 8) plus cisplatin (80 mg per square meter on day 1), administered every 3 weeks for three cycles, plus chemoradiotherapy (concurrent cisplatin at a dose of 100 mg per square meter every 3 weeks for three cycles plus intensity-modulated radiotherapy) or chemoradiotherapy alone. The primary end point was recurrence-free survival (i.e., freedom from disease recurrence [distant metastasis or locoregional recurrence] or death from any cause) in the intention-to-treat population. Secondary end points included overall survival, treatment adherence, and safety. RESULTS: A total of 480 patients were included in the trial (242 patients in the induction chemotherapy group and 238 in the standard-therapy group). At a median follow-up of 42.7 months, the 3-year recurrence-free survival was 85.3% in the induction chemotherapy group and 76.5% in the standard-therapy group (stratified hazard ratio for recurrence or death, 0.51; 95% confidence interval [CI], 0.34 to 0.77; P = 0.001). Overall survival at 3 years was 94.6% and 90.3%, respectively (stratified hazard ratio for death, 0.43; 95% CI, 0.24 to 0.77). A total of 96.7% of the patients completed three cycles of induction chemotherapy. The incidence of acute adverse events of grade 3 or 4 was 75.7% in the induction chemotherapy group and 55.7% in the standard-therapy group, with a higher incidence of neutropenia, thrombocytopenia, anemia, nausea, and vomiting in the induction chemotherapy group. The incidence of grade 3 or 4 late toxic effects was 9.2% in the induction chemotherapy group and 11.4% in the standard-therapy group. CONCLUSIONS: Induction chemotherapy added to chemoradiotherapy significantly improved recurrence-free survival and overall survival, as compared with chemoradiotherapy alone, among patients with locoregionally advanced nasopharyngeal carcinoma. (Funded by the Innovation Team Development Plan of the Ministry of Education and others; ClinicalTrials.gov number, NCT01872962.).


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Quimiorradioterapia , Cisplatino/administração & dosagem , Desoxicitidina/análogos & derivados , Quimioterapia de Indução , Carcinoma Nasofaríngeo/tratamento farmacológico , Adolescente , Adulto , Cisplatino/efeitos adversos , Desoxicitidina/administração & dosagem , Desoxicitidina/efeitos adversos , Feminino , Humanos , Quimioterapia de Indução/efeitos adversos , Leucopenia/induzido quimicamente , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo/terapia , Análise de Sobrevida , Adulto Jovem
12.
Hemoglobin ; 43(1): 7-11, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31084366

RESUMO

Hemoglobinopathies are caused by genetic defects on the globin genes. To date, more than 900 ß-globin variants have been recorded worldwide. These gene alterations often cause either a decrease in ß-globin synthesis or completely block synthesis, leading to a hemoglobinopathy. While most of these causative mutations are inherited, de novo mutations are quite rare. Here, we investigated three hemoglobinopathy cases. These patients developed severe hemolytic anemia at 3-5 months of age and were transfusion-dependent. In patient 1, a novel ß variant, Hb Zunyi [ß147(HC3)Stop→Gln; HBB: c.442T>C] was identified. This de novo mutation results in a stop codon substitution to a glutamine residue at codon 147 of the ß-globin gene, and leads to severe thalassemia. In patient 2, we discovered the rare Hb Southampton mutation [ß106(G8)Leu→Pro; HBB: c.320T>C], while in patient 3, the rare Hb Alesha mutation [ß67(E11)Val→Met (GTG>ATG); HBB: c.202G>A] was detected. The identification of the novel ß variant, Hb Zunyi, has added to the human globin database and will shed light on future diagnosis of hemoglobinopathy/thalassemia and genetic counseling.


Assuntos
Substituição de Aminoácidos , Hemoglobinas Anormais/genética , Mutação , Globinas beta/genética , Talassemia beta/diagnóstico , Talassemia beta/genética , Alelos , Sequência de Aminoácidos , Biomarcadores , Criança , Pré-Escolar , Códon , Análise Mutacional de DNA , Índices de Eritrócitos , Genótipo , Humanos , Masculino , Fenótipo , Índice de Gravidade de Doença
13.
J Org Chem ; 84(7): 4000-4008, 2019 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-30864430

RESUMO

An "on water" organocatalytic cyanoarylmethylation of aryl acetonitrile to isatins is developed, giving products in high yields and up to excellent diastereoselectivities. A remarkable enhancement of reaction rates and diastereoselectivities by water was observed under mild conditions. Moreover, this approach provides a highly efficient and environmentally benign access to thermodynamic 3-hydroxy-3-cyanomethyl oxindoles.

14.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(1): 45-51, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30738446

RESUMO

OBJECTIVE: To investigate the expression of STAT3 gene in patients with acute myeloid leukemia and its correlation with clinical characteristics. METHODS: The real-time quantitative RT-PCR was used to detect the level of STAT3 mRNA in bone marrow samples from 38 newly diagnosed patients with acute myeloid leukemia(AML), and its relevance with clinical characteristics and prognosis were statistically analyzed. Western blot was employed to detect the STAT3 protein level in AML patients. The bone marrow cells from 15 healthy subjects were used as control. RESULTS: At the mRNA level, the expression level of STAT3 in the AML group was significantly higher than that in control group (P<0.05). The level of STAT3 in AML group correlated positively with the risk factors of patients (P<0.01,r=0.592). The STAT3 expression level in the high-risk group was statistically higher than that in the standard-risk group and the control group (P<0.01,P<0.01). Furthermor, there was no statistical difference between the sub-groups of AML (P>0.05). The median survival time of patients in STAT3 low expression group was logner than that in high expression group, but the difference was not statistically significant (P>0.005). The level of STAT3 protein in AML patients was significantly higher than that in control group (P<0.05). CONCLUSION: The STAT3 gene is highly expressed in AML patients, which may be used as a predictor for high-risk of AML.


Assuntos
Leucemia Mieloide Aguda , Fator de Transcrição STAT3/genética , Medula Óssea , Humanos , Prognóstico , RNA Mensageiro
15.
Environ Sci Technol ; 53(3): 1715-1724, 2019 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-30620567

RESUMO

Carbon mitigation strategies have been developed without sufficient consideration of their impacts on the water system. Here, our study evaluates whether carbon mitigation strategies would decrease or increase local industrial water use and water-related pollutants discharge by using a computable general equilibrium (CGE) model coupled with a water withdrawals and pollutants discharge module in Shenzhen, the fourth largest city in China. To fulfill China's Nationally Determined Contributions (NDC) targets, Shenzhen's GDP and welfare losses are projected to be 1.6% and 5.6% in 2030, respectively. The carbon abatement cost will increase from 56 USD/t CO2 in 2020 to 274 USD/t CO2 in 2030. The results reveal that carbon mitigation accelerates local industrial structure upgrading by restricting carbon-, energy-, and water-intensive industries, e.g., natural gas mining, nonmetal, agriculture, food production, and textile sectors. Accordingly, carbon mitigation improves energy use efficiency and decreases 55% of primary energy use in 2030. Meanwhile, it reduces 4% of total industrial water use and 2.2-2.4% of two major pollutants discharge, i.e., CODCr and NH3-N. Carbon mitigation can also decrease petroleum (2.2%) and V-ArOH (0.8%) discharge but has negative impacts on most heavy metal(loid)s pollutants discharge (increased by -0.01% to 4.6%). These negative impacts are evaluated to be negligible on the environment. This study highlights the importance of considering the energy-water nexus for better-coordinated energy and water resources management at local and national levels.


Assuntos
Carbono , Poluentes Ambientais , Dióxido de Carbono , China , Água
16.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 26(6): 1649-1656, 2018 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-30501699

RESUMO

OBJECTIVE: To explore the change of G6PD activity in children with acute leukemia(AL)and its correlation with the clinical characteristics. METHODS: The G6PD activity in peripheral blood samples from 74 children disagnosed as AL (50 cases of ALL, and 24 cases of AML) was detected by Zinkham method recommended by WHO in 1967, and its relevance with clinical indicators was statistically analyzed. The peripheral blood samples of 70 healthy children were used as the controls. RESULTS: The G6PD activity in ALL and AML groups was significantly lower than that in the control group (P=0.000, P=0.000) and there was no statistical difference between ALL and AML groups. The G6PD activity in bacterial, fungal infection and non-infection groups (no bacterial and fungal infection) were statistically different from control group (P=0.02, P=0.001, P=0.001), respectively. The G6PD activity in bacterial infection group and non-infection group was statistically different from with fungal infection group (P=0.004, P=0.019), respectively. The G6PD activity linearly correlated with leukocyte count and neutrophil percentage in AL children (P=0.000, P=0.001, r=0.465, r=0.434), respectively. The median survival time of G6PD activity deficiency group was higher than that in the normal group, but without statistically significant difference (P=0.4149). CONCLUSION: The G6PD activity in AL children is significantly lower than that in healthy children, and the G6PD activity linearly relates with leukocyte count and neutrophil percentage of AL children. The patients with G6PD activity deficiency is more susceptible to fungal infection, moreover the infection is more serious.


Assuntos
Deficiência de Glucosefosfato Desidrogenase , Leucemia Mieloide Aguda , Doença Aguda , Infecções Bacterianas , Criança , Glucosefosfato Desidrogenase , Humanos , Neutrófilos
17.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 26(5): 1430-1436, 2018 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-30295263

RESUMO

OBJECTIVE: To detect the mutation and single nucleotide polymorphisms of STAT3 gene in the patients with myeloproliferative neoplasms (MPN), and to analyze the correlation between STAT3 gene and the subtypes of MPN. METHODS: A total of 147 patients with MPN were selected, including 28 patients with polycythaemia vera (PV), 46 patients with essential thrombocythemia (ET), 10 patients with primary myelofibrosis (PMF), and 63 patients with chronic myeloid leukemia (CML); and 88 healthy persons were used as normal control. DNA of all cases was extracted from bone marrow or peripheral blood, and JAK2V617F gene mutation was detected by allele-specific PCR, then 23 exons of STAT3 gene were amplified by PCR. Mutation and single nucleotide polymorphism of Rs2293152 of STAT3 gene were identified by DNA sequencing. RESULTS: STAT3 gene mutation was found in 8 patients with CML. The mutation rate was 12.7%. the missense mutation(S629T)as found in 3 cases, the synonymous mutation was found in 5 cases (Q469Q 3 cases, G618G 2 cases). One case had mutations at the both sites of S629T and G618G. No mutation of STAT3 gene was found in the normal control group. Rs2293152: detection showed that the G allele of CML group was significantly higher than that of normal control, PV, ET and PMF group (P<0.01), suggesting that the patients with Rs2293152 G allele were more likely to develop CML. The C allele of PV, ET and PMF group was significantly higher than that of CML group (P<0.05), suggesting that the patients with Rs2293152 C allele were more likely to develop PV, ET and PMF. The G allele fiequency of JAK2V617F-negative group was significantly lower than that of the normal control and JAK2V617F positive group (P<0.01), suggesting that the Rs2293152 G allele may be a factor protecting against JAK2V617F mutation. CONCLUSION: In MPN patients, STAT3 gene is unstable and prone to mutation. The different alleles of the Rs2293152 locus of the STAT3 gene are relates with different subtypes and JAK2V617F-negative MPN.


Assuntos
Transtornos Mieloproliferativos , Humanos , Janus Quinase 2 , Mutação , Policitemia Vera , Fator de Transcrição STAT3
18.
J Environ Manage ; 225: 93-103, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30075307

RESUMO

Energy and water systems are interdependent and have complex dynamic interactions with the socio-economic system and climate change. Few tools exist to aid decision-making regarding the management of water and energy resources at a watershed level. In this study, a Computable General Equilibrium (CGE) model and System Dynamics and Water Environmental Model (SyDWEM) were integrated (CGE-SyDWEM) to predict future energy use, CO2 emissions, economic growth, water resource stress, and water quality change in a rapidly urbanizing catchment in China. The effects of both the CO2 mitigation strategies and water engineering measures were evaluated. CO2 mitigation strategies have the potential to reduce 46% CO2 emissions and 41% energy use in 2025 compared with reference scenario. CO2 mitigation strategies are also found to be effective in promoting industrial structure adjustment by decreasing the output of energy- and water-intensive industries. Accordingly, it can alleviate local water stress and improve water environment, including a 4.1% reduction in both domestic water use and pollutant emissions, a 16.8% water demand reduction in the labor-intensive industry sector, and 4.2% and 4.4% decrease in BOD5 and NH3-N loads in all industrial sectors, respectively. It is necessary to implement water engineering measures to further alleviate water resource stress and improve water quality. This study improves the understanding of the feedbacks of CO2 abatement on water demand, pollutant discharges, and water quality improvement. The integrated model developed in this study can be used to aid energy, carbon, and water policy makers to understand the complicated synergistic effects of proposed CO2 mitigation strategies on water demand and pollution emissions, and to design more effective policies and measures to ensure energy and water security in the future.


Assuntos
Carbono , Mudança Climática , Recursos Hídricos , Dióxido de Carbono , China , Água
19.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 26(3): 716-721, 2018 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-29950209

RESUMO

OBJECTIVE: To investigate whether Artesunate(ART) can inhibit the proliferation of THP-1 cells and to explore the potential mechanism of its anti-leukemia effect. METHODS: THP-1 cells were treated with 5 concentrations of Artesunate for 24 h, 48 h or 72 h. The viability of cells was detected with CCK-8 assay, apoptosis was assessed by using flow cytometry, and the STAT3, Caspase3 and Caspase8 protein levels were measured with Western blot . RESULTS: Compared with the control group, ART significantly inhibited the proliferation of THP-1 cells in a dose-dependent manner (r=0.9829, P<0.05). ART also increased the apoptosis of THP-1 cells. The results of Western blot showed that after treated with ART, the STAT3 protein expression in THP-1 cells was significantly down-regulated (P<0.01), and the expressions of Caspase3, cleaved Caspase3 and Caspase8 proteins were up-regulated(P<0.01). CONCLUSION: Artesunate can inhibit the proliferation of THP-1 cells, which may relate with the down-regulation of STAT3 expression and the activation of Capase3 and Caspase8.


Assuntos
Proliferação de Células , Apoptose , Artemisininas , Artesunato , Linhagem Celular Tumoral , Humanos , Células THP-1
20.
Front Chem ; 6: 195, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29911101

RESUMO

Single crystalline fork-like potassium vanadate (K2V8O21) has been successfully prepared by electrospinning method with a subsequent annealing process. The as-obtained K2V8O21 forks show a unique layer-by-layer stacked structure. When used as cathode materials for lithium-ion batteries, the as-prepared fork-like materials exhibit high specific discharge capacity and excellent cyclic stability. High specific discharge capacities of 200.2 and 131.5 mA h g-1 can be delivered at the current densities of 50 and 500 mA g-1, respectively. Furthermore, the K2V8O21 electrode exhibits excellent long-term cycling stability which maintains a capacity of 108.3 mA h g-1 after 300 cycles at 500 mA g-1 with a fading rate of only 0.043% per cycle. The results demonstrate their potential applications in next-generation high-performance lithium-ion batteries.

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