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1.
J Bone Joint Surg Am ; 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36728395

RESUMO

BACKGROUND: Fatty infiltration (FI) of the rotator cuff (RC) muscles is one of the most common risk factors for a retear following RC repair. Recent methodological developments focus on using 3-dimensional measurements of the overall FI of RC muscles instead of using single-plane-based measurements. However, the required labor-intensive segmentation and time-consuming post-processing steps need to be optimized for routine clinical use. METHODS: We collected all 6-point Dixon magnetic resonance imaging (MRI) slices of the overall supraspinatus in 46 patients with atraumatic small-to-massive RC tears. Using emerging techniques, the overall 3-dimensional FI of the supraspinatus (overall FI, using all N slices) was assessed as the reference. Each sagittal segment of the supraspinatus was defined as a sectional accumulation unit (SAU). The localized FI in SAUs with different scales of N/3, N/6, and N/12 slices (SAU-FIs) was then calculated using piecewise accumulation by stacking neighboring slices after the overall supraspinatus had been sequentially segmented using MRI slices of the same thickness. The capacity of the SAU-FIs to predict the overall FI, and the ideal locations for prediction, were evaluated using linear regression models after the associations were examined. Goodness-of-fit of the regression models was appraised by the coefficient of determination (R2) and root-mean-square error (RMSE). The agreement between the predicted and measured overall FI was assessed using Bland-Altman analysis and the standard deviation of the percent differences (sd%). RESULTS: The localized SAU-FIs of the N/3, N/6, and N/12 SAUs generally displayed comparable distributions throughout the normalized distal-proximal long axis of the supraspinatus. The localized SAU-FIs showed substantial correspondence with the overall FI, and the highest correlations were found in the 2/3 SAU (Pearson r and Spearman ρ: 0.95, 0.98), 3/6 and 4/6 SAUs (Pearson r and Spearman ρ: 0.97), and 5/12 to 7/12 SAUs (Pearson r and Spearman ρ: 0.95 to 0.96). The strongest predictors to estimate the overall supraspinatus FI in the regression analysis were these SAU-FIs located in the middle third, which demonstrated good fits to the overall FI (all R2 ≥ 0.90; RMSE ≤ 1.69). The best agreements between the overall FI predicted by the regression models and the measured overall FI were found in these SAUs (2/3 SAU: sd% = 4.84%; 3/6 and 4/6 SAUs: sd% = 5.14%; 5/12 to 7/12 SAUs: sd% = 6.44%). CONCLUSIONS: Specific SAUs near the center of the supraspinatus (2/3, 3/6 and 4/6, and 5/12 to 7/12 SAUs), which displayed the best agreement between the predictions and actual measurements of overall FI values, can serve as appropriate surrogates to estimate the overall FI of the supraspinatus in small-to-massive RC tears. The potential to assess the overall FI of the supraspinatus using specific localized SAUs may improve the speed of analytical strategies for accurately assessing the overall FI of RC muscles and thus enable their routine clinical use in the future. LEVEL OF EVIDENCE: Prognostic Level IV. See Instructions for Authors for a complete description of levels of evidence.

2.
Artigo em Inglês | MEDLINE | ID: mdl-36729118

RESUMO

Electrochemical stability and interfacial reactions are crucial for rechargeable aqueous zinc batteries. Electrolyte engineering with low-cost aqueous electrolytes is highly required to stabilize their interfacial reactions. Herein, we propose a design strategy using glutamic additive and its derivatives with modification of hydrogen-bonding network to enable Zn aqueous battery at a low concentration (2 m ZnSO4 + 1 m Li2SO4). Computational, in situ/ex situ spectroscopic, and electrochemical studies suggest that additives with moderate interactions, such as 0.1 mol % glutamic additive (G1), preferentially absorb on the Zn surface to homogenize Zn2+ plating and favorably interact with Zn2+ in bulk to weaken the interaction between H2O and Zn2+. As a result, uniform deposition and stable electrochemical performance are realized. The Zn||Cu half-cell lasts for more than 200 cycles with an average Coulombic efficiency (CE) of >99.32% and the Zn||Zn symmetrical cells for 1400 h with a low and stable overpotential under a current density of 0.5 mA cm-2, which is better than the reported results. Moreover, adding 0.1 mol % G1 to the Zn||LFP full cell improves its electrochemical performance with stable cycling and achieves a remarkable capacity of 147.25 mAh g-1 with a CE of 99.79% after 200 cycles.

3.
Food Funct ; 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36655680

RESUMO

The threat to human health from cold stimulation is increasing due to the frequent occurrence of temperature extremes. It is a challenge for people to resist the negative effects of prolonged cold stimulation on the heart. In this study, we created prolonged cold stimulation pig models to investigate the cardiac energy metabolism and injury during prolonged cold stimulation, and the molecular mechanisms by which dietary supplementation with full-fat rice bran reduces cardiac injury. The results showed that lesions in the morphological structure of the heart were detected under prolonged cold stimulation. At the same time, dystrophin was downregulated under the effect of prolonged cold stimulation. Cardiac fatty acid transport and utilization were promoted, and oxidative stress was increased under prolonged cold stimulation. It also increased MDA content and decreased T-AOC level in the heart, while promoting the mRNA expression of Nrf2 and NQO1, as well as the protein content of Nrf2 and HO-1. Prolonged cold stimulation induced mitochondrial lesions, mitochondrial fusion, and mitophagy in the heart. Prolonged cold stimulation promoted the mRNA expression of PTGS2, TLR4, MyD88, NLRP3, and IL-1ß; and protein expression of PTGS2, NLRP3, and mature-IL-1ß. GCH1 and FtH inhibited by prolonged cold stimulation caused the activation of heart ferroptosis. In addition, dietary supplementation with full-fat rice bran improved oxidative stress in the heart and inhibited mitophagy, ferroptosis, and pyroptosis. In conclusion, prolonged cold stimulation heightens the risk of cardiac ferroptosis and imbalance of energy metabolism, whereas dietary supplementation with full-fat rice bran mitigates the adverse effects of prolonged cold stimulation on the heart.

4.
Cerebellum ; 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36604400

RESUMO

Cerebellar ataxia(CA) is defined as a degenerative disease of the nervous system. Repetitive transcranial magnetic stimulation (rTMS) has been a promising treatment for neurological and psychiatric diseases. Hence, to find out whether cerebellar rTMS impacts CA as a potential therapy, we performed a systematic review and meta-analysis. Qualified studies through a systematic search were retrieved for randomized controlled trials (RCTs) using acknowledged databases. Review Manager 5.4 software was employed to synthesize the data. A total of seven studies were identified as eligible and included in the quantitative review. Comparing real and sham-rTMS interventions, the utilization of rTMS on cerebellum improved the scale for the assessment and rating of ataxia (SARA) (SMD - 0.87, 95% CI - 1.41 to - 0.34; P = 0.001; I2 = 62%), the International Cooperative Ataxia Rating Scale (ICARS) (SMD - 1.06, 95% CI - 1.47 to - 0.64; P < 0.00001; I2 = 0%) and Berg balance Scale (BBS) (SMD 0.76, 95% CI 0.33 to 1.19; P = 0.0005; I2 = 39%). The subgroup analysis demonstrated high-frequency of rTMS had a positive effect (SMD - 1.28, 95% CI - 1.82 to - 0.74; P < 0.00001; I2 = 0%). For the safety, the incidence of adverse events between the two groups was not significantly different (OR 1.73, 95% CI 0.55 to 5.46; P = 0.35; I2 = 0%). In conclusion, this meta-analysis provided limited evidence, suggesting a possible strategy that rTMS over the cerebellum could be a viable therapy for symptoms associated with CA. Besides, rTMS intervention was well-attended and did not result in unanticipated negative effects.

5.
Langenbecks Arch Surg ; 408(1): 15, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36622474

RESUMO

PURPOSE: Safety in creating a preperitoneal space is crucial in laparoscopic totally extraperitoneal (TEP) hernia repairs. In this systematic review and meta-analysis, we compared the outcomes of balloon dissection and telescopic dissection in patients with inguinal or femoral hernias who underwent TEP hernia repair. METHODS: We searched PubMed, Embase, Web of Science, and Cochrane databases for randomized controlled trials (RCTs) and prospective and retrospective studies published from inception to July 2022. Meta-analysis was performed using a random-effects model. The treatment outcome was measured using operation time, incidence of intraoperative hemorrhage, peritoneal laceration, conversion to other approaches, surgical site infection (SSI), hematoma, seroma formation, hernia recurrence, and postoperative pain. RESULTS: Five RCTs, one prospective study, and two retrospective studies (in total, 936 patients) were included. No significant between-group differences were noted in operation time, SSI, hematoma, seroma, recurrence rate, and postoperative pain on days 1 and 7. The conversion rate was significantly lower in the balloon group than in the telescopic group (odds ratio, 0.34; 95% confidence interval, 0.15-0.81). CONCLUSIONS: Both balloon dissection and telescopic dissection are viable techniques for creating preperitoneal space in laparoscopic TEP hernia repair and have similar operation time, complication rate, and postoperative pain. Nevertheless, the conversion rate was lower in patients undergoing balloon dissection than in those undergoing telescopic dissection.


Assuntos
Hérnia Inguinal , Laparoscopia , Humanos , Herniorrafia/efeitos adversos , Herniorrafia/métodos , Seroma/etiologia , Seroma/cirurgia , Hérnia Inguinal/cirurgia , Laparoscopia/efeitos adversos , Laparoscopia/métodos , Resultado do Tratamento , Dor Pós-Operatória , Telas Cirúrgicas/efeitos adversos
6.
Carbohydr Polym ; 302: 120349, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36604043

RESUMO

The design and development of smart shape-adaptable wound dressings with superior antimicrobial ability remain a challenge in therapeutic and clinical application. Herein, polydopamine/tannic acid/chitosan/poloxamer 407/188 hydrogel (PTCPP) was prepared with the aim of developing an in situ-formed antibacterial wound dressing with temperature stimulation and near-infrared radiation (NIR) responsive ability. PTCPP possessed injectability, photothermal stability, sustained release properties and cytocompatibility. In vitro antibacterial results showed that the bactericidal rates of PTCPP against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) under NIR irradiation were 99.994 % and 99.91 %, respectively. In vivo experiments, PTCPP can adapt to shape of the wound, showing good adhesion, while promoting wound healing in bacterial infections. Therefore, PTCPP has potential application in the treatment of infectious wounds, and provides a strategic choice for developing antibacterial wound dressing combined with photothermal therapy.


Assuntos
Quitosana , Infecção dos Ferimentos , Humanos , Quitosana/farmacologia , Hidrogéis/farmacologia , Poloxâmero , Staphylococcus aureus , Escherichia coli , Antibacterianos , Cicatrização , Infecção dos Ferimentos/tratamento farmacológico
7.
Sleep Med Rev ; 68: 101743, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36657366

RESUMO

Obstructive sleep apnea (OSA) is a significant healthcare burden affecting approximately one billion people worldwide. The prevalence of OSA is rising with the ongoing obesity epidemic, a key risk factor for its development. While in-laboratory polysomnography (PSG) is the gold standard for diagnosing OSA, it has significant drawbacks that prevent widespread use. Portable devices with different levels of monitoring are available to allow remote assessment for OSA. To better inform clinical practice and research, this comprehensive systematic review evaluated diagnostic performances, study cost and patients' experience of different levels of portable sleep studies (type 2, 3, and 4), as well as wearable devices and non-contact systems, in adults. Despite varying study designs and devices used, portable diagnostic tests are found to be sufficient for initial screening of patients at risk of OSA. Future studies are needed to evaluate cost effectiveness with the incorporation of portable diagnostic tests into the diagnostic pathway for OSA, as well as their application in patients with chronic respiratory diseases and other comorbidities that may affect test performance.

8.
BMC Med Educ ; 23(1): 49, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36690973

RESUMO

Learning effectiveness may be affected by internal and external factors, including personal attitude, motivations, learning skills, learning environment and peer pressure. This study sought to explore potential factors on students who majored in medical technology. The 106 students who completed their internship at Chang Gung Memorial Hospital were enrolled in this study. A written questionnaire was analyzed to explore the relationship between potential factors and learning effectiveness. The strength of relationship between the outcome and each factor was evaluated using Spearman correlation coefficients. A multiple linear regression model was constructed to assess how those factors affected learning effectiveness altogether. The results indicated that the learning effectiveness of the students mainly depended on three factors: the "extracurricular studies" and "willingness to cooperate" were positively associated with learning effectiveness. However, the "weakened motivation due to uncertainty" is negatively associated with learning effectiveness. We suggested that the educators can understand the uncertainty of students about the future. Additionally, the projects that require joint cooperation and discussion need to be given. The most important thing is that students should be able to integrate the learning content instead of rote.


Assuntos
Aprendizagem , Estudantes de Medicina , Humanos , Estudantes , Motivação , Currículo , Inquéritos e Questionários
9.
Hepatol Int ; 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36701081

RESUMO

BACKGROUND: Real-world data are scarce about the effectiveness and safety of sofosbuvir/velpatasvir/voxilaprevir (SOF/VEL/VOX) for retreating East Asian patients with hepatitis C virus (HCV) infection who previously received NS5A direct-acting antivirals (DAAs). We conducted a multicenter study to assess the performance of SOF/VEL/VOX in patients who were not responsive to prior NS5A inhibitors in Taiwan. METHODS: Between September 2021 and May 2022, 107 patients who failed NS5A inhibitor-containing DAAs with SOF/VEL/VOX salvage therapy for 12 weeks were included at 16 academic centers. The sustained virologic response at off-treatment week 12 (SVR12) was assessed in the evaluable (EP) and per-protocol (PP) populations. The safety profiles were also reported. RESULTS: All patients completed 12 weeks of treatment and achieved an end-of-treatment virologic response. The SVR12 rates were 97.2% (95% confidence interval (CI) 92.1-99.0%) and 100% (95% CI 96.4-100%) in EP and PP populations. Three (2.8%) patients were lost to off-treatment follow-up and did not meet SVR12 in the EP population. No baseline factors predicted SVR12. Two (1.9%) not-fatal serious adverse events (AE) occurred but were unrelated to SOF/VEL/VOX. Sixteen (15.0%) had grade 2 total bilirubin elevation, and three (2.8%) had grade 2 alanine transaminase (ALT) elevation. Thirteen (81.3%) of the 16 patients with grade 2 total bilirubin elevation had unconjugated hyperbilirubinemia. The estimated glomerular filtration rates (eGFR) were comparable between baseline and SVR12, regardless of baseline renal reserve. CONCLUSIONS: SOF/VEL/VOX is highly efficacious and well-tolerated for East Asian HCV patients previously treated with NS5A inhibitor-containing DAAs. CLINICAL TRIALS REGISTRATION: The study was not a drug trial. There was no need for clinical trial registration.

10.
J Mater Chem B ; 11(5): 1159, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36651517

RESUMO

Correction for 'Exploration of biomimetic poly(γ-benzyl-L-glutamate) fibrous scaffolds for corneal nerve regeneration' by Tien-Li Ma et al., J. Mater. Chem. B, 2022, 10, 6372-6379, https://doi.org/10.1039/D2TB01250B.

11.
BMJ Open ; 13(1): e066356, 2023 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-36631236

RESUMO

INTRODUCTION: Balance impairments frequently occur after stroke. Achieving effective core trunk stability is the key to improving balance ability. However, there is still a lack of advanced well-defined rehabilitation protocols for balance improvement in patients with stroke. Intermittent theta-burst stimulation (iTBS) is a non-invasive brain activity modulation strategy that can produce long-term potentiation. The cerebellar vermis is a fundamental structure involved in balance and motor control. However, no study has demonstrated the therapeutic effect and potential mechanism of cerebellar vermis iTBS on balance after stroke. METHODS AND ANALYSIS: This study will be a prospective single-centre double-blind randomised controlled clinical trial with a 3-week intervention and 3-week follow-up. Eligible participants will be randomly allocated to the experimental group or the control group in a 1:1 ratio. After routine conventional physical therapy, patients in the experimental group will receive cerebellar vermis iTBS, whereas patients in the control group will receive sham stimulation. The overall intervention period will be 5 days a week for 3 consecutive weeks. The outcomes will be measured at baseline (T0), 3 weeks postintervention (T1) and at the 3-week follow-up (T2). The primary outcomes are Berg Balance Scale and Trunk Impairment Scale scores. The secondary outcomes are balance test scores via the Balance Master system, muscle activation of the trunk and lower limbs via the surface electromyography recordings, cerebral cortex oxygen concentrations measured via the resting-state functional near-infrared spectroscopy, Fugl-Meyer Assessment of Lower Extremity and Barthel index scores. ETHICS AND DISSEMINATION: This study was approved by the West China Hospital Clinical Trials and Biomedical Ethics Committee of Sichuan University. All participants will sign the informed consent form voluntarily. The results of this study will be published in peer-reviewed journals and disseminated at academic conferences. TRIAL REGISTRATION NUMBER: ChiCTR2200065369.


Assuntos
Vermis Cerebelar , Reabilitação do Acidente Vascular Cerebral , Acidente Vascular Cerebral , Humanos , Reabilitação do Acidente Vascular Cerebral/métodos , Estimulação Magnética Transcraniana/métodos , Estudos Prospectivos , Acidente Vascular Cerebral/terapia , Resultado do Tratamento , Ensaios Clínicos Controlados Aleatórios como Assunto
12.
J Oncol ; 2023: 2439128, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36644232

RESUMO

Aims: An adjuvant oxaliplatin-based regimen is the standard of care for patients with stage III colorectal cancer (CRC). Few reports have compared the clinicopathological features and oncological outcomes of such treatment between patients with early (≤1 year) and late recurrence (>1 year). Methods: Between January 2012 and December 2019, CRC recurred in 128 (24.1%) of 531 patients with consecutive stage III CRC after they received curative resection and an adjuvant oxaliplatin-based regimen. The clinicopathological features and oncological outcomes of the 128 patients were analyzed retrospectively. Results: The median follow-up period after the first chemotherapy cycle was 35.0 months (range, 7-100.9), and the median recurrence time was 16.1 months. Forty-seven patients (36.7%) had an early recurrence and eighty-one patients (63.3%) had a late recurrence. Compared with patients with late recurrence, those with early recurrence were mostly younger (median: 58 vs. 64 years, p=0.009), had less oxaliplatin-based therapy cycles (median: 8 vs. 12 cycles, p < 0.001), and had a shorter overall survival time (median: 23.3 vs. 39.7 months, p < 0.001). The area under the curve of patient age and chemotherapy cycles for predicting early recurrence was 0.629 and 0.705 (p=0.015 and p < 0.001), respectively. The receiver operating characteristic curve analysis demonstrated that the cutoff level for patient age was 57 years and the number of chemotherapy cycles was 8. A multivariate analysis revealed that patient age ≤57 years and oxaliplatin-based therapy ≤8 cycles were independent risk factors for early recurrence (odds ratio (OR) = 3.049, p=0.022; OR = 4.995, p=0.002). These factors were associated with an approximately 77.8% risk of recurrence within 1 year, compared with the 21.5% risk associated with patient age >57 years and oxaliplatin-based therapy >8 cycles (p = 0.003). Conclusion: Patients with early recurrence had poorer survival than those with late recurrence. If >8 cycles of oxaliplatin-based therapy can be administered without disease progression, then patients with stage III CRC would have a lower risk of early recurrence.

13.
Sci Total Environ ; 865: 161063, 2023 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-36586676

RESUMO

Chlorinated volatile organic compounds (CVOCs) are still a part of the current atmospheric environmental problems that cannot be ignored, but unlike conventional VOCs, the presence of Cl causes various catalyst deactivations in the catalytic process. In this paper, we focus on six common CVOCs and discuss various behavioral mechanisms of the whole catalytic process from six aspects: catalyst selection, factors affecting the catalytic effect, changes in catalytic behavior in the presence of different gases, catalyst poisoning deactivation behavior, degradation products and degradation mechanisms to provide guidance for further development of low-temperature and efficient CVOCs catalysts.

14.
Int J Biol Macromol ; 228: 706-714, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36584777

RESUMO

CRISPR-Cas, as a tool for gene editing, has received extensive attention in recent years. Anti-CRISPR (Acr) proteins can inactivate the CRISPR-Cas defense system during interference phase, and can be used as a potential tool for the regulation of gene editing. In-depth study of Anti-CRISPR proteins is of great significance for the implementation of gene editing. In this study, we developed a high-accuracy prediction model based on two-step model fusion strategy, called AcrPred, which could produce an AUC of 0.952 with independent dataset validation. To further validate the proposed model, we compared with published tools and correctly identified 9 of 10 new Acr proteins, indicating the strong generalization ability of our model. Finally, for the convenience of related wet-experimental researchers, a user-friendly web-server AcrPred (Anti-CRISPR proteins Prediction) was established at http://lin-group.cn/server/AcrPred, by which users can easily identify potential Anti-CRISPR proteins.


Assuntos
Sistemas CRISPR-Cas , Edição de Genes , Sistemas CRISPR-Cas/genética , Algoritmos , Aprendizado de Máquina , Proteínas Virais/genética
15.
Int J Biol Macromol ; 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36470433

RESUMO

RNA N4-acetylcytidine (ac4C) is the acetylation of cytidine at the nitrogen-4 position, which is a highly conserved RNA modification and involves a variety of biological processes. Hence, accurate identification of genome-wide ac4C sites is vital for understanding regulation mechanism of gene expression. In this work, a novel predictor, named iRNA-ac4C, was established to identify ac4C sites in human mRNA based on three feature extraction methods, including nucleotide composition, nucleotide chemical property, and accumulated nucleotide frequency. Subsequently, minimum-Redundancy-Maximum-Relevance combined with incremental feature selection strategies was utilized to select the optimal feature subset. According to the optimal feature subset, the best ac4C classification model was trained by gradient boosting decision tree with 10-fold cross-validation. The results of independent testing set indicated that our proposed method could produce encouraging generalization capabilities. For the convenience of other researchers, we established a user-friendly web server which is freely available at http://lin-group.cn/server/iRNA-ac4C/. We hope that the tool could provide guide for wet-experimental scholars.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36464206

RESUMO

PURPOSE: To investigate whether preoperative skin cleaning of the chin, neck, and chest with chlorhexidine soap could reduce suture contamination by Cutibacterium acnes (C. acnes) in patients undergoing arthroscopic rotator cuff repair. METHODS: This study included patients who underwent primary arthroscopic rotator cuff repair. Exclusion criteria included age <18 years, previous shoulder surgery, history of shoulder infection, and allergy to chlorhexidine. Patients were randomized into three groups. Patients in the control group cleaned their skin with soap and water, as usual. Patients in the shoulder group cleaned their shoulders with chlorhexidine soap 3 days before surgery, whereas patients in the extended shoulder group additionally cleaned their chest, back, neck, and face with chlorhexidine soap. On the day of surgery, skin swab samples were obtained from the shoulder after surgical draping. After rotator cuff repair, sutures were cut from the anchor ends. Both traditional culture methods and polymerase chain reaction (PCR) were used. RESULTS: Ninety patients were enrolled (32 in the control group, 29 in the shoulder group, and 29 in the extended shoulder group) in the present study. The culture-positive rate from the posterior shoulder skin samples in the extended shoulder group (17.2%) was significantly lower than that in the control (40.6%) and shoulder (48.3%) groups (P = 0.036), whereas the culture-positive rates were not different among the three groups in other skin samples as well as the suture samples. The detection rates of C. acnes in suture samples were 12.5%, 13.8%, and 17.2% in the control, shoulder, and extended shoulder groups, respectively (P = 0.603). CONCLUSION: Extensive skin cleaning to the shoulder region with chlorhexidine helps reduce the shoulder cutaneous bacterial load, but the detection of C. acnes suture contamination in patients undergoing arthroscopic rotator cuff repair remained untouched regardless of the use of chlorhexidine soap in skin cleaning on the preoperative days.

17.
Front Immunol ; 13: 1040947, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466831

RESUMO

Objectives: Human mitochondrial cell-free DNA (Mt-cfDNA) may serve as a useful biomarker for infectious processes. We investigated Mt-cfDNA dynamics in patients with pulmonary mycobacterial infections to determine if this novel biomarker could be used to differentiate disease states and severity. Methods: Patients with pulmonary tuberculosis (PTB), latent tuberculosis infection (LTBI), and nontuberculous mycobacterial-lung disease (NTM-LD) were enrolled at a tertiary care hospital in Taiwan between June 2018 and August 2021. Human Mt-cfDNA and nuclear-cfDNA (Nu-cfDNA) copy numbers were estimated by quantitative polymerase chain reaction. Variables associated with PTB and 2-month sputum culture-positivity, indicating poor treatment response, were assessed using logistic regression. Results: Among 97 patients with PTB, 64 with LTBI, and 51 with NTM-LD, Mt-cfDNA levels were higher in patients with PTB than in LTBI (p=0.001) or NTM-LD (p=0.006). In the Mycobacterium tuberculosis-infected population, Mt-cfDNA levels were highest in smear-positive PTB patients, followed by smear-negative PTB (p<0.001), and were lowest in LTBI persons (p=0.009). A Mt-cfDNA, but not Nu-cfDNA, level higher than the median helped differentiate culture-positive PTB from culture-negative PTB and LTBI (adjusted OR 2.430 [95% CI 1.139-5.186], p=0.022) and differentiate PTB from NTM-LD (adjusted OR 4.007 [1.382-12.031], p=0.011). Mt-cfDNA levels decreased after 2 months of treatment in PTB patients (p=0.010). A cutoff Mt-cfDNA level greater than 62.62 x 106 copies/µL-plasma was associated with a 10-fold risk of 2-month culture-positivity (adjusted OR 9.691 [1.046-89.813], p=0.046). Conclusion: Elevated Mt-cfDNA levels were associated with PTB disease and failed sputum conversion at 2 months in PTB patients, and decreased after treatment.


Assuntos
Ácidos Nucleicos Livres , Tuberculose Latente , Infecções por Mycobacterium não Tuberculosas , Pneumonia , Tuberculose Pulmonar , Humanos , Ácidos Nucleicos Livres/genética , Dinâmica Mitocondrial , Tuberculose Pulmonar/diagnóstico , Tuberculose Pulmonar/tratamento farmacológico , Tuberculose Pulmonar/genética , Infecções por Mycobacterium não Tuberculosas/diagnóstico , Biomarcadores , Tuberculose Latente/diagnóstico , Tuberculose Latente/genética
18.
Materials (Basel) ; 15(23)2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36499886

RESUMO

The use of phase change materials (PCMs) in concrete is a double-edged sword that improves the thermal inertia but degrades the mechanical properties of concrete. It has been an essential but unsolved issue to enhance the thermal capacity of PCMs while non-decreasing their mechanical strength. To this end, this work designs a novel 3D printing phase change aggregate to prepare concrete with prominent thermal capacity and ductility. The work investigated the effects of 3D printing phase change aggregate on the compressive strength and splitting tensile strength of concrete. The compressive strength of phase change aggregate concrete is 21.18 MPa, but the ductility of concrete improves. The splitting tensile strength was 1.45 MPa. The peak strain is 11.69 × 10-3, nearly 13 times that of basalt aggregate concrete. Moreover, using 3D printing phase change aggregate reduced concrete's early peak hydration temperature by 7.1%. The thermal insulation capacity of the experiment cube model with phase change concrete has been improved. The results show that the novel 3D printing change aggregate concrete has good mechanical properties and latent heat storage, providing a guideline for applying PCMs in building materials.

19.
Int Immunopharmacol ; 115: 109564, 2022 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-36571916

RESUMO

Macrophages, a versatile subset of immune cells, are essential for successful bone repair. Hydrogen sulfide (H2S) is a gasotransmitter associated with tissue development and repair. Emerging evidence demonstrates that H2S is involved in bone formation under physiology condition and bone regeneration under pathology condition. However, whether hydrogen sulfide mediates osteogenesis by influencing macrophages is unknown. Here, we aimed to investigate the effects of hydrogen sulfide on macrophage polarization and the subsequent impact on bone regeneration. In the present study, we found that the H2S-donor GYY4137 stimulated M0/M1 macrophages to express high level of CD-206 and IL-10 but decreased the levels of i-NOS and TNF-α in M1 macrophages. Furthermore, coculture of GYY4137-treated M0 macrophages with pro-osteoblastic MC3T3-E1 cells significantly increased the viability of the MC3T3-E1 cells. Importantly, the formation of mineralized particles in MC3T3-E1 cells was significantly promoted following coculture with IL-4-treated and GYY4137-treated M0 macrophages. Collectively, our study demonstrated that hydrogen sulfide increased macrophages M2 polarization and subsequently promoted bone regeneration.

20.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 30(6): 1679-1687, 2022 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-36476889

RESUMO

OBJECTIVE: To explore the hematopoiesis protection effect of Danggui Buxue Tang (DBT) and its main components Angelica polysaccharide (APS) and Astragalus polysaccharide (ASPS) on myelosuppression mice, and the mechanism of anti-apoptosis of Meg-01 cells. METHODS: Mice were radiated with 4 Gy of 137Csγ ray to establish the model of radiation-induced myelosuppression. DBT, APS or ASPS (10 mg/kg) were injected into irradiated mice. Peripheral blood cell counts were performed on mice before radiation (day 0) and day 7, 14 and 21 after radiation. On the 21st day, poor plasma platelets were collected from mice to detect TPO concentration and then the mice were sacrificed. The femoral bone marrow cells were cultured for colony cell forming units (CFU). Meg-01 cells were cultured without FBS for 24 h to induce apoptosis, and then treated with DBT/APS/ASPS for 72 h. Flow cytometry (FCM) was used to detect early apoptosis (Annexin V), mitochondrial membrane potential (JC-1) and the expression of Caspase-3 to analyze the effect of DBT/APS/ASPS on cell apoptosis. RESULTS: DBT can stimulate the recovery of white blood cells (WBC), red blood cells (RBC) and platelets (PLT) of myelosuppression mice, especially for WBC and PLT (P<0.01, P<0.05). Compared with the control group, the number of BFU-E, CFU-MK and CFU-GM increased after adding DBT (BFU-E & CFU-GM: P<0.05; CFU-MK: P<0.01). The effect of DBT on blood TPO concentration in mice was not obvious (P=0.89). RBC, WBC and PLT were increased in APS group compared with control group (P<0.05). WBC increased after the treatment of ASPS (P<0.05). APS stimulated the formation of CFU-F, CFU-MK and CFU-GM (P<0.05). Only CFU-GM increased in ASPS group(P<0.05). Besides, DBT decreased the apoptosis of Meg-01 cells (P<0.05). The early apoptosis rate and total death rate in APS (100 µg/ml) group were lower than that of control group (P<0.01, P<0.05). The early apoptosis rate of ASPS (100 µg/ml) group was lower than that of control group (P<0.05). JC-1 and Caspase-3 showed that APS (100 µg/ml) significantly reduced apoptosis rate (P<0.01, P<0.05). CONCLUSION: DBT has protective effect on hematopoietic system, especially WBC and PLT, and has anti-apoptotic effect on Meg-01. It was found that the above effects of DBT were mainly caused by APS, and its anti-apoptosis mechanism was carried out mainly through JC-1 and Caspase-3 pathways.


Assuntos
Medula Óssea , Polissacarídeos , Camundongos , Animais , Caspase 3
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