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1.
Sci Total Environ ; 803: 149754, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34496344

RESUMO

The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 °C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 °C to 23 °C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 °C to 32 °C. Anammox activity was severely inhibited below 12.5 °C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 °C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application.


Assuntos
Microbiota , Esgotos , Anaerobiose , Reatores Biológicos , Desnitrificação , Nitrogênio , Oxirredução , Temperatura
2.
Chemosphere ; 288(Pt 1): 132390, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34600013

RESUMO

The nitrogen removal characteristics, physicochemical properties, and microbial community composition of four different anaerobic ammonium oxidation (anammox) sludge morphologies were investigated. The morphologies considered in this study, namely suspended sludge (Rs), biofilm (Rm), granular sludge (Rg), and encapsulated biomass (Re), were prepared from floc sludge. The results show that Re exhibited the maximum anammox activity, followed by Rg, Rm, and Rs. Additionally, the anammox contribution rate was higher in Rg and Re. The higher extracellular polymer content in Rg promoted sludge accumulation, and tryptophan was observed in Rm and Rg, which was replaced by humic acids in Rs. Re showed the largest specific surface area, hydrophobicity and strength, and its good structure ensured enrichment of anammox bacteria (AnAOB). In terms of the microbial community, the functional bacterium Candidatus Kuenenia accounted for the highest proportion in Rm (39.27%), but the presence of both anaerobic and aerobic regions led to increased community complexity with more nitrifying bacteria. In contrast, Rg and Re had a more specific microbial community. In addition, denitrifying bacteria tended to grow in Rs, while nitrifying bacteria were retained in Rm. The AnAOB were more likely to be enriched in sludge aggregates (both Rm and Rg) and carriers (Re). Through correlation analysis, the potential relationship involving bacterial flora evolution of each sample was clarified. Finally, the structural models of different morphologies of sludge were proposed. This study deepens the understanding of various anammox sludge morphologies as well as provides useful information for the cultivation of AnAOB and further application of anammox.

3.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120327, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34474220

RESUMO

In this study, a classification model was established based on near-infrared spectroscopy and random forest method to accurately distinguish three samples of Schisandra chinensis from different habitats. At the same time, the feasibility of fast and effective prediction of polysaccharide contents in Schisandra chinensis by near-infrared spectroscopy combined with chemometrics was evaluated. In this paper, phenol sulfuric acid method was used to determine the content of total polysaccharides in samples, and partial least squares regression algorithm was used to link the spectral information with the reference value. Different spectral pretreatment methods were used to optimize the model to improve its predictability and stability. The results showed that random forest could distinguish these samples accurately, with an accuracy of 97.47%. In the established prediction model, the RMSEC of the optimal model calibration set is 0.0012, and the coefficient of determination R is 0.9976. The RMSEP of prediction set is 0.0024, the coefficient of determination R is 0.9922, and the RPD is 11.36. In general, the method has good stability and applicability, which provides a new analytical method for the identification of Schisandra chinensis origin and quality evaluation.


Assuntos
Schisandra , Espectroscopia de Luz Próxima ao Infravermelho , Calibragem , Análise dos Mínimos Quadrados , Polissacarídeos
4.
Artigo em Inglês | MEDLINE | ID: mdl-34812574

RESUMO

Two-dimensional/three-dimensional (2D/3D) mixed perovskites combine the advantages containing superior stability of 2D perovskite and excellent efficiency of 3D perovskite by introducing organic spacer cations. However, the effects from the molecular configuration of diammonium spacer cations on 2D/3D perovskite properties are still unclear. Here, we firstly investigated systematically the mechansim of molecular configuration-induced regulation of crystallization kinetic and carrier dynamics by employing various diammonium molecules to construct Dion-Jacobson (DJ)-type 2D/3D perovskites to further facilitating the photovoltaic performance. The minimum average Pb-I-Pb angle leads to the smallest octahedral tilting of [PbX6]4-  lattice in optimal diammonium molecule-incorporated DJ-type 2D/3D perovskite, which enables suitable binding energy and hydrogen-bonding between spacer cations and inorganic [PbX6]4- cages, thus contributing to the formation of high-quality perovskite film with vertical crystal orientation, mitigatory lattice distortion and efficient carrier transportation. As a consequence, a dramatically improved device efficiency of 22.68% is achieved with excellent mositure stability.

5.
Pharmazie ; 76(11): 523-527, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34782035

RESUMO

Background: The exact pathogenesis of psoriasis is complex, and scholars use the intestinal mucosal immunity as an entry point to analyze the important role of the intestine in the pathogenesis of psoriasis. Traditional Chinese medicine treats psoriasis from the intestine based on the theory that "the lung governs the fur" and "the Interior-Exterior Relationship Between the Lung and Large Intestine". Based on this understanding, this paper puts forward the idea of understanding psoriasis from the "gut-skin" axis. Objective: Based on the "gut-skin" axis to explore the pathogenesis of psoriasis from the intestines, and open up new ideas for research and development of new drugs for psoriasis. Method: Collect literature on the treatment of psoriasis from the perspective of the intestine and "gut-skin" axis; then, use Western medicine's intestinal pathogenesis, Traditional Chinese Medicine (TCM) theory and examples of TCM treatment are demonstrated; finally, the treatment of psoriasis from the "gut-skin" axis is summarized. Results: Western medicine has not carried out treatment of psoriasis involving the intestinal tract. In-depth research and clinical applications based on the "gut-skin" axis are still needed. The effective rate of treating psoriasis by TCM has been as high as 90%, but the mechanism research is relatively scarce. Conclusion: The construction of the "gut-skin" axis mechanism is consistent with TCM theories, and is consistent with modern scientific connotations as well.

6.
J Am Heart Assoc ; 10(20): e022304, 2021 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-34612058

RESUMO

Background Circulating microRNAs are emerging biomarkers for heart failure (HF). Our study aimed to assess the prognostic value of microRNA signature that is differentially expressed in patients with acute HF. Methods and Results Our study comprised a screening cohort of 15 patients with AHF and 5 controls, a PCR-discovery cohort of 50 patients with AHF and 26 controls and a validation cohort of 564 patients with AHF from registered study DRAGON-HF (Diagnostic, Risk Stratification and Prognostic Value of Novel Biomarkers in Patients With Heart Failure). Through screening by RNA-sequencing and verification by reverse-transcription quantitative polymerase chain reaction, 9 differentially expressed microRNAs were verified (miR-939-5p, miR-1908-5p, miR-7706, miR-101-3p, miR-144-3p, miR-4732-3p, miR-3615, miR-484 and miR-19b-3p). Among them, miR-19b-3p was identified as the microRNA signature with the highest fold-change of 8.4 and the strongest prognostic potential (area under curve with 95% CI, 0.791, 0.654-0.927). To further validate its prognostic value, in the validation cohort, the baseline level of miR-19b-3p was measured. During a follow-up period of 19.1 (17.7, 20.7) months, primary end point comprising of all-cause mortality or readmission due to HF occurred in 48.9% patients, while patients in the highest quartile of miR-19b-3p level presented the worst survival (Log-rank P<0.001). Multivariate Cox model showed that the level of miR-19b-3p could independently predict the occurrence of primary end point (adjusted hazard ratio,1.39; 95% CI, 1.18-1.64). In addition, miR-19b-3p positively correlated with soluble suppression of tumorigenicity 2 and echocardiographic indexes of left ventricular hypertrophy. Conclusions Circulating miR-19b-3p could be a valuable prognostic biomarker for AHF. In addition, a high level of circulating miR-19b-3p might indicate ventricular hypertrophy in AHF subjects. Registration URL: https://www.clinicaltrials.gov. Unique Identifier: NCT03727828.

7.
Opt Express ; 29(19): 30655-30665, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34614786

RESUMO

Snapshot multispectral imaging (MSI) has been widely employed in the rapid visual inspection by virtues of the non-invasive detection mode and short integration time. As the critical functional elements of snapshot MSI, narrowband, customizable, and pixel-level multispectral filter arrays (MSFAs) that are compatible with imaging sensors are difficult to be efficiently manufactured. Meanwhile, monolithically integrating MSFAs into snapshot multispectral imagers still remains challenging considering the strict alignment precision. Here, we propose a cost-efficient, wafer-level, and customized approach for fabricating transmissive MSFAs based on Fabry-Perot structures, both in the pixel-level and window-tiled configuration, by utilizing the conventional lithography combined with the deposition method. The MSFA chips own a total dimension covering the area of 4.8 mm × 3.6 mm with 4 × 4 bands, possessing the capability to maintain narrow line widths (∼25 nm) across the whole visible frequencies. After the compact integration with the imaging sensor, the MSFAs are validated to be effective in filtering and target identification. Our proposed fabrication method and imaging mode show great potentials to be an alternative to MSFAs production and MSI, by reducing both complexity and cost of manufacturing, while increasing flexibility and customization of imaging system.

8.
Nucleic Acids Res ; 49(19): 11224-11240, 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34606619

RESUMO

The human RecQ helicase BLM is involved in the DNA damage response, DNA metabolism, and genetic stability. Loss of function mutations in BLM cause the genetic instability/cancer predisposition syndrome Bloom syndrome. However, the molecular mechanism underlying the regulation of BLM in cancers remains largely elusive. Here, we demonstrate that the deubiquitinating enzyme USP37 interacts with BLM and that USP37 deubiquitinates and stabilizes BLM, thereby sustaining the DNA damage response (DDR). Mechanistically, DNA double-strand breaks (DSB) promotes ATM phosphorylation of USP37 and enhances the binding between USP37 and BLM. Moreover, knockdown of USP37 increases BLM polyubiquitination, accelerates its proteolysis, and impairs its function in DNA damage response. This leads to enhanced DNA damage and sensitizes breast cancer cells to DNA-damaging agents in both cell culture and in vivo mouse models. Collectively, our results establish a novel molecular mechanism for the USP37-BLM axis in regulating DSB repair with an important role in chemotherapy and radiotherapy response in human cancers.

9.
Pharmazie ; 76(10): 473-479, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34620273

RESUMO

High-calorie food intake and unhealthy lifestyle have considerably raised the incidence of obesity. The gut microbiota is associated with metabolic disorders which promote excessive accumulation of fat, elicit host systemic, low-grade chronic inflammation, and insulin resistance. These features make gut microbiota a promising target for the prevention and intervention of obesity. Herbal polysaccharides in TCM are uneasy to digest by the host, but can be decomposed and utilized by the intestinal flora as the carbon source to mediate bacterial composition. In this review, we are describing the therapeutic effect of polysaccharides on the imbalanced structure of gut microbiota and its metabolites, and discuss the research progress that has been made to unravel the underlying mechanism by which polysaccharides of TCM inhibit the onset and development of obesity.

10.
Nat Mater ; 20(10): 1306-1307, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34561626
11.
Chem Commun (Camb) ; 57(80): 10335-10338, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34519291

RESUMO

A spinel-alumina inert oxide supported gold catalyst with high Au dispersion and excellent CO oxidation activity was developed by a deposition-precipitation method. The activation atmosphere could tune the reaction pathway by adjusting the amount of surface adsorbed water species, thus transforming the reaction intermediates from HCO3- or CO32- to COOH.

12.
ACS Appl Nano Mater ; 4(8): 8455-8464, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34485846

RESUMO

In many porous catalyst supports, the accessibility of interior catalytic sites to reactant species could be restricted due to limitations of reactant transport through pores comparable to reactant dimensions. The interplay between reaction and diffusion in porous catalysts is defined through the Thiele modulus and the effectiveness factor, with diffusional restrictions leading to high Thiele moduli, reduced effectivess factors, and a reduction in the observed reaction rate. We demonstrate a method to integrate ceramic nanostraws into the interior of ordered mesoporous silica MCM-41 to mitigate diffusional restrictions. The nanostraws are the natural aluminosilicate tubular clay minerals known as halloysite. Such halloysite nanotubes (HNTs) have a lumen diameter of 15-30 nm, which is significantly larger than the 2-4 nm pores of MCM-41, thus facilitating entry and egress of larger molecules to the interior of the pellet. The method of integrating HNT nanostraws into MCM-41 is through a ship-in-a-bottle approach of synthesizing MCM-41 in the confined volume of an aerosol droplet that contains HNT nanotubes. The concept is applied to a system in which microcrystallites of Ni@ZSM-5 are incorporated into MCM-41. Using the liquid phase reduction of nitrophenol as a model reaction catalyzed by Ni@ZSM-5, we show that the insertion of HNT nanostraws into this composite leads to a 50% increase in the effectiveness factor. The process of integrating nanostraws into MCM-41 through the aerosol-assisted approach is a one-step facile method that complements traditional catalyst preparation techniques. The facile and scalable synthesis technique toward the mitigation of diffusional restrictions has implications to catalysis and separation technologies.

13.
J Nutr ; 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34590120

RESUMO

BACKGROUND: Current evidence on tea consumption and hypertension is inconclusive, and prospective studies among habitual tea drinkers remain limited. OBJECTIVE: We investigated the associations of habitual tea consumption with hypertension risk and longitudinal blood pressure changes in 2 large cohorts. METHODS: This study included participants aged 40-75 y from the Shanghai Women's Health Study (n = 31,351) and the Shanghai Men's Health Study (n = 28,342), without hypertension, diabetes, cardiovascular disease, or cancer at baseline. Information on tea consumption was assessed during in-person interviews at enrollment and follow-up visits. Incident hypertension was identified by self-reported diagnosis, medication use, or blood pressure measurements. RESULTS: Current tea drinkers had a 7% higher risk than the non-current tea drinker group [HRs (95% CIs): women, 1.07 (1.01, 1.14); men, 1.07 (1.02, 1.12)]. The amount of tea drinking showed significant dose-response associations with hypertension: compared with the non-current group, HRs (95% CIs) for women and men were 1.01 (0.90, 1.14) and 1.02 (0.96, 1.08) for low (women/men: <100/200 g/mo), 1.07 (1.01, 1.15) and 1.05 (0.99, 1.12) for medium (women/men: 100-250/200-250 g/mo), and 1.18 (1.01, 1.39) and 1.10 (1.03, 1.17) for the high-amount group (women/men: >250 g/mo). Among participants without hypertension, compared with non-current tea drinkers, least-squares means of 3-y changes in blood pressure were 0.3-0.4 mm Hg higher for women and men as current drinkers and 0.7-0.9 mm Hg higher for men in the high-consumption group. Compared with those who never drank tea, women who drank tea consistently had 0.5 (0.2, 0.7) mm Hg higher diastolic blood pressure (DBP), whereas men had 0.5 (0.04, 0.9) mm Hg higher systolic blood pressure and 0.3 (0.04, 0.6) mm Hg higher DBP, respectively. CONCLUSIONS: Our findings suggest that habitual tea drinking is associated with a slightly higher risk of hypertension and a minor increase in blood pressure among middle-aged and older Chinese adults, which warrants confirmation by long-term intervention studies.

14.
Artigo em Inglês | MEDLINE | ID: mdl-34501927

RESUMO

The vegetation landscape in urban green space has been shown to provide great psychological benefits to people. Flower border is a well-designed small-scale vegetation landscape with the advantages of color and vegetation richness. This study focused on the effects of the visual attributes of flower borders on the aesthetic preference and emotional perception. The face recognition measurement method was used to obtain the emotional perception and the questionnaire survey method was used to measure the aesthetic preference. The results indicated the following: (1) regarding the 'color features' factor, high proportions of cool color and green vegetation significantly increased aesthetic preference and emotional valence, while the proportion of warm color had a negative effect on valence; (2) the 'visual attractiveness' (color brightness, and visual richness) and 'color configuration' (number of plant patches and number of color hues) factor was positively associated with aesthetic preference and emotional valence; (3) aesthetic preference was significantly related to emotional valence; (4) males expressed higher aesthetic preference and valence for flower border images than females. The results are expected to improve the aesthetic quality of flower borders and to promote public emotional health through the effective design of urban vegetation landscapes.


Assuntos
Emoções , Flores , Estética , Humanos , Parques Recreativos , Percepção , Percepção Visual
15.
mBio ; 12(5): e0209921, 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34544273

RESUMO

The two-component system PhoP/PhoQ is essential for Salmonella enterica serovar Typhimurium virulence. Here, we report that PhoP is methylated extensively. Two consecutive glutamate (E) and aspartate (D)/E residues, i.e., E8/D9 and E107/E108, and arginine (R) 112 can be methylated. Individual mutation of these above-mentioned residues caused impaired phosphorylation and dimerization or DNA-binding ability of PhoP to a different extent and led to attenuated bacterial virulence. With the help of specific antibodies recognizing methylated E8 and monomethylated R112, we found that the methylation levels of E8 or R112 decreased dramatically when bacteria encountered low magnesium, acidic pH, or phagocytosis by macrophages, under which PhoP can be activated. Furthermore, CheR, a bacterial chemotaxis methyltransferase, was identified to methylate R112. Overexpression of cheR decreased PhoP activity but increased PhoP stability. Together, the current study reveals that methylation plays an important role in regulating PhoP activities in response to environmental cues and, consequently, modulates Salmonella virulence. IMPORTANCE Posttranslational modifications (PTMs) play an important role in regulating enzyme activities, protein-protein interactions, or DNA-protein recognition and, consequently, modulate many biological functions. We demonstrated that PhoP, the response regulator of PhoP/PhoQ two-component system, could be methylated on several evolutionally conserved amino acid residues. These amino acid residues were crucial for PhoP phosphorylation or dimerization, DNA-binding ability of PhoP, and Salmonella virulence. Interestingly, methylation negatively regulated the activity of PhoP. A bacterial chemotaxis methyltransferase CheR was involved in PhoP methylation. Methylation of PhoP could stabilize it in an inactive conformation. Our work provides a more informative depiction of PhoP PTM and markedly improves our understanding of the coordinate regulation of bacterial chemotaxis and virulence.

16.
Nat Commun ; 12(1): 5447, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34521830

RESUMO

Prohibiting deep oxidation remains a challenging task in oxidative dehydrogenation of light alkane since the targeted alkene is more reactive than parent substrate. Here we tailor dual active sites to isolate dehydrogenation and oxidation instead of homogeneously active sites responsible for these two steps leading to consecutive oxidation of alkene. The introduction of HY zeolite with acid sites, three-dimensional pore structure and supercages gives rise to Ni2+ Lewis acid sites (LAS) and NiO nanoclusters confined in framework wherein catalytic dehydrogenation of ethane occurs on Ni2+ LAS resulting in the formation of ethene and hydrogen while NiO nanoclusters with decreased oxygen reactivity are responsible for selective oxidation of hydrogen rather than over-oxidizing ethene. Such tailored strategy achieves near 100% ethene selectivity and constitutes a promising basis for highly selective oxidation catalysis beyond oxidative dehydrogenation of light alkane.

17.
PLoS Pathog ; 17(8): e1009735, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34347835

RESUMO

Whooping cough is resurging in the United States despite high vaccine coverage. The rapid rise of Bordetella pertussis isolates lacking pertactin (PRN), a key vaccine antigen, has led to concerns about vaccine-driven evolution. Previous studies showed that pertactin can mediate binding to mammalian cells in vitro and act as an immunomodulatory factor in resisting neutrophil-mediated clearance. To further investigate the role of PRN in vivo, we examined the functions of pertactin in the context of a more naturally low dose inoculation experimental system using C3H/HeJ mice that is more sensitive to effects on colonization, growth and spread within the respiratory tract, as well as an experimental approach to measure shedding and transmission between hosts. A B. bronchiseptica pertactin deletion mutant was found to behave similarly to its wild-type (WT) parental strain in colonization of the nasal cavity, trachea, and lungs of mice. However, the pertactin-deficient strain was shed from the nares of mice in much lower numbers, resulting in a significantly lower rate of transmission between hosts. Histological examination of respiratory epithelia revealed that pertactin-deficient bacteria induced substantially less inflammation and mucus accumulation than the WT strain and in vitro assays verified the effect of PRN on the induction of TNF-α by murine macrophages. Interestingly, only WT B. bronchiseptica could be recovered from the spleen of infected mice and were further observed to be intracellular among isolated splenocytes, indicating that pertactin contributes to systemic dissemination involving intracellular survival. These results suggest that pertactin can mediate interactions with immune cells and augments inflammation that contributes to bacterial shedding and transmission between hosts. Understanding the relative contributions of various factors to inflammation, mucus production, shedding and transmission will guide novel strategies to interfere with the reemergence of pertussis.


Assuntos
Células Epiteliais Alveolares/microbiologia , Proteínas da Membrana Bacteriana Externa/metabolismo , Derrame de Bactérias , Infecções por Bordetella/transmissão , Bordetella bronchiseptica/patogenicidade , Inflamação/patologia , Fatores de Virulência de Bordetella/metabolismo , Animais , Aderência Bacteriana , Proteínas da Membrana Bacteriana Externa/genética , Infecções por Bordetella/metabolismo , Infecções por Bordetella/microbiologia , Feminino , Humanos , Inflamação/metabolismo , Inflamação/microbiologia , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Fatores de Virulência de Bordetella/genética
18.
Orthop Surg ; 13(6): 1922-1933, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34423576

RESUMO

OBJECTIVE: To highlight the characteristics of the most highly cited articles and propose the research interests over the past decades in the field of femoroacetabular impingement (FAI) and labral tear. METHODS: The ISI Web of Science database (Clarivate Analytics, New York, the United States) was utilized for the identification of articles on 15 December 2020. FAI and labral tear-related articles (1138 articles) were retrieved, of which the 100 most-cited articles (top 100) were identified. Subsequent analysis included citation density (citations/article age), authorship, institution, journal, geographic distribution, level of evidence, and theme. RESULTS: The number of citations per article ranged from 66 to 1189 with a mean of 163.31. The majority of articles were published in the United States (all articles/top 100 = 655/57) and Switzerland (85/22). University of Bern (n = 10) was the most prolific institution. The journal with the most of articles was Arthroscopy: The Journal of Arthroscopic and Related Surgery. The most prolific coauthor (all articles) or first authors (top 100) was Domb (n = 109) and Philippon (n = 6), respectively. The evidence with the most articles is level IV (n = 41). The top three most popular topics of research article were outcomes of surgery (n = 23), imaging diagnosis (n = 18), and comparison of surgery (n = 8). The top four most prevalent themes of review were labral tears (n = 3), FAI (n = 3), comparison of surgery imaging diagnosis, and outcomes of surgery (both n = 2). Six keywords with the newest average publication year, including FAI syndrome (average publication year = 2019.50), patient-reported outcomes (2019.43), femoroplasty (2018.60), clinical outcomes (2018.17), borderline dysplasia (2018.00), and capsule (2018.00). Five keywords with the highest average citations, including outcome (average citations = 88.50), alpha angle (58.00), complications (55.86), revision hip arthroscopy (49.00), and systematic review (46.14). CONCLUSIONS: Outcomes research is the most popular research interest and patient-reported outcome instruments might be further and widely used in the emerging articles in the near future. The field of FAI and labral tear has shown an obvious trend of development and is steadily evolving. It could be predicted that there will be an increasing number of publications in the following years, with the United States and Switzerland maintaining leadership in this field.


Assuntos
Autoria , Cartilagem Articular/diagnóstico por imagem , Cartilagem Articular/cirurgia , Impacto Femoroacetabular/diagnóstico por imagem , Impacto Femoroacetabular/cirurgia , Publicações Periódicas como Assunto , Publicações/tendências , Bibliometria , Cartilagem Articular/lesões , Humanos , Medidas de Resultados Relatados pelo Paciente
19.
Hum Cell ; 34(6): 1866-1877, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34346032

RESUMO

Circular RNAs (circRNAs) have been documented to be aberrantly expressed in many types of malignancies and involved in cancer progression. However, their role in thyroid cancer (TC) remains largely unknown. Our study aimed to explore the role and mechanism of circUBAP2 in TC. The differentially expressed circRNAs in TC tissues were identified using GSE18105 from gene expression omnibus (GEO) database. CircUBAP2 and miR-370-3p expression was analyzed using qRT-PCR. The stability of circUBAP2 was confirmed by actinomycin D and RNase R. The subcellular localization of circUBAP2 was detected using cell fractionation assay. Cell proliferation, apoptosis, and invasion were evaluated using MTT, flow cytometry analysis, and Transwell invasion assay, respectively. The interaction between circUBAP2 and miR-370-3p was predicted using bioinformatics analysis and validated by luciferase reporter assay, RNA pull-down assay, and RNA immunoprecipitation. CircUBAP2 was upregulated and miR-370-3p was downregulated in TC tissues and cells. CircUBAP2 was highly stable, resistant to RNase R digestion, and predominantly localized in the cytoplasm. CircUBAP2 knockdown inhibited cell proliferation and invasion and triggered apoptosis in TC cells. Bioinformatics analysis showed that circUBAP2 contained putative binding sites of miR-370-3p. CircUBAP2 acted as a sponge to inhibit miR-370-3p expression. Mechanistically, miR-370-3p inhibition abolished the effects of circUBAP2 on proliferation, apoptosis, and invasion in TC cells. Taken together, CircUBAP2 knockdown impeded the proliferation and invasion and induced apoptosis in TC cells via sponging miR-370-3p.

20.
Front Cardiovasc Med ; 8: 683418, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34368245

RESUMO

This review summarizes current knowledge regarding clinical epidemiology, pathophysiology, and prognosis for patients with HFmrEF in comparison to HFrEF and HFpEF. Although recommended treatments currently focus on aggressive management of comorbidities, we summarize potentially beneficial therapies that can delay the process of heart failure by blocking the pathophysiology mechanism. More studies are needed to further characterize HFmrEF and identify effective management strategies that can reduce cardiovascular morbidity and mortality of patients with HFmrEF.

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