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1.
Int J Biol Macromol ; 219: 1155-1162, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36057294

RESUMO

Bacillus velezensis P1 inhibits various phytopathogenic fungi. Its exopolymeric substance (EPS) was active against Colletotrichum sp. at a minimum inhibitory concentration (MIC) of 6.25 mg/ml. EPS was added to chitosan from squid pen to produce an antifungal film coating. The addition of EPS at its MIC had no effect on the color of the chitosan film. Two components showed good compatibility and the tensile strength of the film was higher than that of neat chitosan film. Mangoes were treated with six different coatings, including the two films. Fruits coated with the films showed the lowest weight loss and prolonged shelf life of mangoes to three times the shelf life of controls (water and 2 % citric acid, w/v) and completely inhibited fungal infection. Film coatings of chitosan, alone or combined with the EPS from B. velezensis P1, showed potential to prolong the shelf life of ripe mangoes and decrease postharvest losses.


Assuntos
Quitosana , Mangifera , Antifúngicos/farmacologia , Bacillus , Quitosana/farmacologia , Ácido Cítrico , Conservação de Alimentos , Frutas , Água
2.
Arch Microbiol ; 204(9): 563, 2022 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-35982281

RESUMO

A Gram-negative, motile, rod-shaped, aerobic bacterial strain CAU 1632T was isolated from a seawater sample collected in the Republic of Korea. The cells of strain CAU 1632T grew optimally at 30 °C and pH 7.0 in 0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CAU 1632T formed a tight phyletic lineage with Zhongshania antarctica ZS-23T, Zhongshania borealis CL-AS9T, Zhongshania marina DSW25-10T, Zhongshania guokunii ZS6-22T, 'Zhongshania ponticola GM-8T', Zhongshania aliphaticivorans SM-2T and shared a high similarity between 97.2% and 97.7%. The whole genome of strain CAU 1632T was 4.3-Mb with 3,780 protein-coding genes, 12 contigs, and a DNA G+C content of 49.4 mol%. The major fatty acids of strain CAU 1632T were C17:1 ω8c, C19:1 ω6c and/or C19:0 cyclo ω10c (summed feature 3), and C18:1 ω6c and/or C18:1 ω7c (summed feature 8). Q-8 was the only respiratory quinone. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, and two phospholipids. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analyses, strain CAU 1632T represents a novel species within the genus Zhongshania, for which the name Zhongshania aquimaris sp. nov. is proposed. The type strain is CAU 1632T (= KCTC 82432T = MCCC 1K06086T).


Assuntos
Fosfolipídeos , Água do Mar , Técnicas de Tipagem Bacteriana , DNA Bacteriano/química , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA
3.
Arch Microbiol ; 204(9): 567, 2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-35984503

RESUMO

A Gram-negative, nonmotile, rod-shaped bacterium, designated strain CAU 1631T, was isolated from a mudflat sample in the Republic of Korea. Strain CAU 1631T grew optimally at 30 °C, pH 6.5, and 1% (w/v) NaCl solution. Phylogenetic analysis based on 16S rRNA gene sequencing and 92 core genes indicated that strain CAU 1631T is a member of the genus Muricauda and most closely related to Muricauda oceanensis 40DY170T and Muricauda lutimaris SMK-108T (98.1%, both). The draft genome was 3.4 Mb with 3064 protein-coding genes, and the DNA G + C content was 43.3 mol%. The major fatty acids were iso-C15:0, iso-C17:0 3-OH, and iso-C15:0 G, and the major polar lipid was phosphatidylethanolamine. The predominant respiratory quinone was MK-6. Based on the comprehensive taxonomic characterization, strain CAU 1631T is a novel species, for which the name Muricauda lutisoli sp. nov. has been proposed. The type strain is CAU 1631T (= KCTC 82456T = MCCC 1K06088T).


Assuntos
Ácidos Graxos , Água do Mar , Técnicas de Tipagem Bacteriana , DNA Bacteriano/química , DNA Bacteriano/genética , Ácidos Graxos/análise , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Vitamina K 2/análise
4.
Arch Microbiol ; 204(8): 517, 2022 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-35870024

RESUMO

A Gram-negative, strictly aerobic, non-motile, rod-shaped bacterial strain CAU 1614T was isolated from a marine sediment sample collected in the Republic of Korea. Optimal growth of strain CAU 1614T proceeded at 30 °C, pH 7.0, and 2% (w/v) NaCl. 16S rRNA gene similarity was lower than 94.5% with genera Aureisphaera, Marinirhabdus, Aureitalea, Gilvibacter, Ulvibacter, and Jejudonia. The highest similarity was with Aureisphaera galaxeae 04OKA003-7T (94.5%). The major cellular fatty acids were iso-C15:0, iso-C16:0, iso-C15:1 G, iso-C16:0 3-OH, and iso-C17:0 3-OH and the predominant menaquinone was MK-6. The polar lipids were phosphatidylethanolamine, phosphoglycolipid, an unidentified lipid, two unidentified aminolipids, and an unidentified glycolipid. The draft genome of strain CAU 1614T was 3.9 Mb and DNA G+C content was 36.0 mol%. On the basis of the phenotypic, chemotaxonomic, and genomic data, strain CAU 1614T presents a novel genus in the family Flavobacteriaceae, for which the name Halomarinibacterium sedimenti gen. nov., sp. nov. is proposed. The type strain is CAU 1614T (= KCTC 82457T = MCCC 1K06083T).


Assuntos
Sedimentos Geológicos , Água do Mar , Técnicas de Tipagem Bacteriana , Carotenoides , DNA Bacteriano/genética , Ácidos Graxos/análise , Sedimentos Geológicos/microbiologia , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Vitamina K 2/química
5.
Arch Microbiol ; 204(7): 409, 2022 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-35729359

RESUMO

A Gram-negative, aerobic, rod-shaped bacterium, designated strain CAU 1522T, was isolated from a sea sand sample collected from the Republic of Korea. Optimal growth of strain CAU 1522T ensued at 30 °C, pH 7.5, and 1.0% (w/v) NaCl. Strain CAU 1522T was affiliated to the genus Thalassococcus with high similarity to T. lentus KCTC 32084T (97.5%), T. profundi MCCC 1K03253T (96.5%), and T. halodurans JCM 13833T (96.1%) according to phylogenetic analysis based on 16S rRNA gene sequences. The whole genome of strain CAU 1522T was 3.7 Mb in length and included 7 contigs and 3599 protein-coding genes, with a G + C content of 65.4 mol%. The predominant cellular fatty acids were C18:1 ω6c and/or C18:1 ω7c (summed feature 8), with Q-10 being the sole isoprenoid quinone. The polar lipids included phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, and an unidentified lipid. These phenotypic, chemotaxonomic, and phylogenetic data support that strain CAU 1522T is a novel Thalassococcus species, for which the name T. arenae sp. nov. is proposed. The type strain is CAU 1522T (= KCTC 72545T = MCCC 1K04064T).


Assuntos
Rhodobacteraceae , Areia , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/química
6.
Curr Microbiol ; 79(7): 201, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-35596013

RESUMO

A Gram-negative, strictly aerobic, rod-shaped, non-spore-forming bacterium, designated CAU 1555T, was isolated from a sediment sample collected on Jeju Island, Republic of Korea. Growth of the isolate was observed at 20-37 °C (optimum at 30 °C) and pH 5.5-10.0 (optimum at 8.0). Phylogenetic analysis based on the result of 16S rRNA gene sequences revealed that strain CAU 1555T belonged to the genus Thauera and was closely related to Thauera hydrothermalis GD-2T (98.4% sequence similarity), Thauera lacus D20T (96.6%), and Thauera linaloolentis 47LolT (95.5%). Strain CAU 1555T possessed phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, and one aminophospholipid as the major polar lipids; Q-8 as the predominant respiratory quinone; and C16:0, summed feature 3 (comprising C16:1ω6c and/or C16:1ω7c), and summed feature 8 (comprising C18:1 ω7c/ C18:1 ω6c) as the major fatty acids. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the new isolate and T. hydrothermalis GD-2T were 84.5%, 86.4%, and 28.0%, respectively. Whole-genome sequencing of strain CAU 1555T revealed 3,955,289 bp with a DNA G + C content of 68.0 mol%. Based on the results of its polyphasic properties and genomic analysis, the isolate represents a novel species within the genus Thauera, for which the name Thauera sedimentorum sp. nov. is proposed, with CAU 1555T (= KCTC 72546T = MCCC 1K04065T) as the type strain.


Assuntos
Fosfolipídeos , Thauera , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Thauera/genética
7.
Arch Microbiol ; 204(5): 276, 2022 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-35460040

RESUMO

A Gram-negative, aerobic, motile, rod-shaped bacterium, designated strain CAU 1594T, was isolated from a coastal sand sample collected in the Republic of Korea. Cells of strain CAU 1594T grew best at 30 °C, pH 7.5, and in the presence of 1% (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequencing, indicated that strain CAU 1594T was affiliated with the genus Marinobacterium and most similar to Marinobacterium jannaschii ATCC 27135T (95.1%) and Marinobacterium stanieri ATCC 27130T (94.9%). The whole genome of strain CAU 1594T was 4,917,683 bp, including 4,188 CDSs, with a 60.4 mol% G + C content. Based on draft genome sequences, the average nucleotide identity and digital DNA-DNA hybridization values of strain CAU 1594T were within the ranges of 71.9-73.1% and 20.0-2.1%, respectively, compared to reference strains. The major respiratory quinone was ubiquinone-8, and the major fatty acids were C16:0, summed feature 3 (C16:1 ω6c and C16:1 ω7c), and summed feature 8 (C18:1 ω6c and C18:1 ω7c). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on these results, strain CAU 1594T represents a novel species of the genus Marinobacterium, for which the name Marinobacterium arenosum sp. nov. is proposed. The type strain is CAU 1594T (=KCTC 82405T=MCCC 1K05672T).


Assuntos
Alteromonadaceae , Areia , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
8.
Artigo em Inglês | MEDLINE | ID: mdl-35103586

RESUMO

A novel bacterium, designated CAU 1612T, was isolated from coastal sediment sampled in the Republic of Korea. Cells of the strain were Gram-stain-negative, rod-shaped and motile. Optimum growth occurred at 30 °C, at pH 7.0 and with 1.0 % (w/v) NaCl. Based on 16S rRNA gene sequences, phylogenetic analysis revealed that strain CAU 1612T belonged to the genus Sneathiella and showed highest similarity to 'Sneathiella litorea' JCM 33810T (98.5 %), followed by Sneathiella chungangensis KCTC 32476T (97.7 %), Sneathiella aquimaris KCTC 12842T (96.0 %), Sneathiella chinensis LMG 23452T (95.5 %), Sneathiella limimaris KCTC 52846T (95.5 %) and Sneathiella glossodoripedis KCTC 12842T (95.4 %). The average nucleotide identity and digital DNA-DNA hybridization values of strain CAU 1612T were estimated to be 68.5-81.7 % and ≤23.7 %, respectively, to members of the genus Sneathiella. The DNA G+C content was 54.1 mol%. The major cellular fatty acids (>10 %) were C16:0, C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major menaquinone was Q-10 and the predominant polar lipids were phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. On the basis of taxonomic evidence, strain CAU 1612T represents a novel species, for which the name Sneathiella sedimenti sp. nov. is proposed. The type strain is CAU 1612T (=KCTC 82427T=MCCC 1K06082T).


Assuntos
Alphaproteobacteria/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Água do Mar/microbiologia , Análise de Sequência de DNA , Vitamina K 2/química
9.
Arch Microbiol ; 204(1): 113, 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34982225

RESUMO

In this study, strain CAU 1523T, a novel Gram-positive-positive bacterium isolated from marine sediment collected from the coast of Busan, Republic of Korea, was characterized using a polyphasic taxonomic approach. This strain showed growth at a temperature range of 20-37 °C (optimum, 30 °C), a pH range of 6.5-9.5 (optimum, 7.5), and in the presence of 0-3% (w/v) NaCl (optimum, 1%). Phylogenetic analysis based on 16S rRNA gene sequencing and 92 concatenated core genes indicated that CAU 1523T belonged to the genus Paenibacillus, sharing the highest sequence similarity with P. assamensis JCM 13186T (98.0%). CAU 1523T was differentiated from other Paenibacillus species by average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values, using cut-off values of 95-96%, 90%, and 70%, respectively, for closely related strains. The genome of CAU 1523T possessed various biosynthetic gene clusters, one of which encoded a putative siderophore-interacting protein. Siderophore production by the isolate was confirmed using the qualitative chrome azurol sulfonate (CAS) agar assay. Based on its phylogenetic and physiological characteristics, strain CAU 1523T represents a novel, siderophore-producing species within the genus Paenibacillus, for which the name Paenibacillus arenosi sp. nov. is proposed, with the type strain CAU 1523T (= KCTC 43108T = MCCC 1K04063T).


Assuntos
Paenibacillus , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos , Paenibacillus/genética , Fosfolipídeos , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Sideróforos
10.
Arch Microbiol ; 204(2): 147, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-35059852

RESUMO

A Gram-negative, non-motile, short rod-shaped aerobic bacterial strain CAU 1593T was isolated from a coastal sand sample collected in the Republic of Korea. Cells of strain CAU 1593T grew optimally at 30 °C and pH 7.5 in 4% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CAU 1593T had the highest similarity to Arenibacterium halophilum (97.5%). The whole genome of strain CAU 1593T was 3,979,826 bp with 26 contigs, and the DNA G + C content was 64.3 mol%. The major fatty acid of strain CAU 1593 T was summed feature 8 (C18:1 ω7c/C18:1 ω6c). Q-10 was the only respiratory quinone. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two phosphoglycolipids, an unidentified glycolipid, and an unidentified lipid. Based on the results of chemotaxonomic, phylogenetic, and phenotypic analyses, strain CAU 1593T represents a novel species in the genus Arenibacterium, which is named Arenibacterium arenosum sp. nov. The type strain is CAU 1593T (= KCTC 82402T = MCCC 1K05671T).


Assuntos
Fosfolipídeos , Areia , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Dióxido de Silício , Ubiquinona
11.
Arch Microbiol ; 204(2): 155, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-35094174

RESUMO

A Gram-negative, rod-shaped, motile bacterium, designated strain CAU 1620T, was isolated from a tidal flat sediment in Incheon, Republic of Korea. Strain CAU 1620T grew optimally at 30 °C and pH 8.0 in the presence of 6.0% (w/v) NaCl. The results of 16S rRNA gene sequence analysis revealed that strain CAU 1620T showed the highest similarity to Marinobacter adhaerens DSM 23420T (98.5%), followed by Marinobacter algicola DSM 16394T (98.3%) and Marinobacter maroccanus LMG 30465T (98.2%). The average nucleotide identity and digital DNA-DNA hybridisation values between strain CAU 1620T and related strains were estimated as 75.6-78.1% and 19.5-20.9%, respectively. The DNA G + C content based on the draft genome sequence was 59.2%, and the major respiratory quinone was ubiquinone-9. The predominant cellular fatty acids were C12:0, C16:0, C18:1 ω9c, and C12:0 3OH. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. According to phenotypic, genotypic, and chemotaxonomic analyses, strain CAU 1620T represents a novel species of the genus Marinobacter, for which the name Marinobacter arenosus sp. nov. is proposed. The type strain is CAU 1620T (= KCTC 82431T = MCCC 1K06079T).


Assuntos
Marinobacter , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Marinobacter/genética , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
12.
Int J Syst Evol Microbiol ; 71(12)2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34908522

RESUMO

A novel Gram-negative, aerobic, non-motile, rod-shaped, bacterial strain (CAU 1598T) was isolated from marine sand. Strain CAU 1598T grew well at 30 °C, pH 6.5-7.0 and with 3 % NaCl (w/v). Phylogeny results based on 16S rRNA gene sequencing indicated that the identified strain had the highest similarity (94.3%) to Pseudoxanthomonas putridarboris, indicating that strain CAU 1598T belongs to the family Xanthomonadaceae. Further, the fatty acid profile of the strain was primarily composed of C16:0, iso-C15 : 0, iso-C16 : 0, summed feature 3 (consisting of C16 : 1 ω7c/iso-C15 : 0 2-OH) and summed feature 9 (consisting of iso-C17 : 1 ω9c and/or C16 : 0 10-methyl), with ubiquinone-8 as the major isoprenoid quinone. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, an unidentified aminolipid and an unidentified lipid. The G+C content of the bacterial genome was 62.6 mol% and its 5.4 Mb length encompassed 144 contigs and 4236 protein-coding genes. These phenotypic, chemotaxonomic and phylogenetic data indicate that CAU 1598T belongs to a new genus and species, for which the name Pseudomarimonas arenosa gen. nov., sp. nov. is proposed. The type strain is CAU 1598T (=KCTC 82406T=MCCC 1K05673T).


Assuntos
Filogenia , Areia , Xanthomonadaceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Areia/microbiologia , Água do Mar , Análise de Sequência de DNA , Ubiquinona/química , Xanthomonadaceae/isolamento & purificação
13.
Artigo em Inglês | MEDLINE | ID: mdl-34841569

RESUMO

Purple nonsulfur bacteria (PNSB) were investigated for their carotenoid production and anti-vibrio activity against acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus. To test carotenoid production, selected strains were cultivated in basic isolation medium (BIM), glutamate acetate medium, G5 medium and artificial acetic acid wastewater (AAW) medium. From 144 PNSB, Rhodopseudomonas palustris KTSSG46 was selected to produce carotenoids under microaerobic light conditions in BIM. When the culture medium was optimized, strain KTSSG46 grown in BIM modified with l-glutamate at 1 g/L more effectively inhibited AHPND-causing V. parahaemolyticus strains than standard BIM with 1 g/L (NH4 )2 SO4 . BIM was further modified with 1.23 g/L MgSO4 ·7H2 O and carotenoid production increased 40.22%. Carotenoid production at day 2 by strain KTSSG46 grown in BIM modified with l-glutamate at 1 and 1.23 g/L MgSO4 ·7H2 O was the same as production in BIM modified with monosodium glutamate (MSG). Culture supernatants from all BIM formulations showed similar activity against the resistant AHPND strain SR2. Based on high-performance liquid chromatography, carotenoids of strain KTSSG46 might be canthaxanthin. Grown in BIM modified with MSG, strain KTSSG46 could produce inexpensive carotenoids and release anti-vibrio compounds that, applied as shrimp feed additive, would prevent AHPND strains.

14.
Int J Syst Evol Microbiol ; 71(11)2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34846293

RESUMO

A novel bacterium, designated strain CAU 1637T, was isolated from a tidal mudflat. Cells of strain CAU 1637T were Gram-stain-negative, aerobic, motile with single flagellum and rod-shaped. The optimum conditions for growth were observed at 30 °C, pH 6.0 and in the presence of 2 % (w/v) NaCl. The respiratory quinone was ubiquinone-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1637T was closely related to the genus Roseibium, with the highest similarity to Roseibium aestuarii NRBC 112946T (97.4 %), followed by Roseibium hamelinense NRBC 16783T (96.8 %), Roseibium aquae JCM 19310T (96.4 %), Roseibium sediminis KCTC 52373T (95.8 %) and Roseibium denhamense JCM 10543T (95.3 %). The predominant cellular fatty acids were C18 : 1 ω7c 11-methyl and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity values between the novel isolate and related strains ranged from 71.0 to 76.4 %, and the DNA-DNA hybridization values ranged from 19.3 to 20.3 %. The G+C content was 58.4 mol% and the whole-genome size was 4.6 Mb, which included 17 contigs and 3931 protein-coding genes. Based on the taxonomic data, strain CAU 1637T represents a novel species of the genus Roseibium, for which the name Roseibium limicola sp. nov. is proposed. The type strain is CAU 1637T (=KCTC 82429T=MCCC 1K06080T).


Assuntos
Alphaproteobacteria/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
15.
Int J Syst Evol Microbiol ; 71(10)2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34605765

RESUMO

A Gram-stain-negative, aerobic, motile, short rod-shaped, catalase-negative and oxidase-positive bacterium, strain CAU 1568T, was isolated from marine sediment sand sampled at Sido Island in the Republic of Korea. The optimum conditions for growth were at 25-30 °C, at pH 6.5-8.5 and with 0-4.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAU 1568T was a member of the genus Photobacterium with high similarity to Photobacterium salinisoli JCM 30852T (97.7 %), Photobacterium halotolerans KACC 17089T (97.3 %) and Photobacterium galatheae LMG F28894T (97.3 %). The predominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), with Q-8 as the major of isoprenoid quinone. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerols, phosphatidylcholine, phosphatidylethanolamine, phospholipid, two aminophospholipids and three unidentified lipids. The whole genome size of strain CAU 1568T was 4.8 Mb with 50.1 mol% G+C content; including 38 contigs and 4233 protein-coding genes. These taxonomic data support CAU 1568T as representing a novel Photobacterium species, for which the name Photobacterium arenosum sp. nov. is proposed. The type strain of this novel species is CAU 1568T (=KCTC 82404T=MCCC 1K05668T).


Assuntos
Photobacterium , Filogenia , Areia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Sedimentos Geológicos/microbiologia , Ilhas , Fosfolipídeos/química , Photobacterium/classificação , Photobacterium/isolamento & purificação , RNA Ribossômico 16S/genética , República da Coreia , Areia/microbiologia , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/química
16.
Arch Microbiol ; 203(9): 5437-5443, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34405260

RESUMO

A Gram stain-negative, aerobic, motile by gliding, rod-shaped bacterial strain CAU 1569T was isolated from marine sediment on Shido Island in Incheon. It grew at 20-37 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, 7.0), 2-6% NaCl (w/v) (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CAU 1569T formed a distinct lineage with only the type strain of Snuella. Strain CAU 1569T showed high similarity to S. lapsa KACC 14152T (95.8%), Mariniflexile gromovii KMM KCTC 12570T, Aestuariibaculum marinum KCTC 52521T (95.4%), A. suncheonense KACC 16186T (94.6%) and Yeosuana aromativorans KCCM 42019T (94.4%). The genome contained 57 contigs, 3,437 protein-coding gene, 3 rRNAs (5, 16, and 23S), 43 tRNAs, and with a 35.7 mol% G + C content. The DDH value between strain CAU 1569T and S. lapsa KACC 14152T was 39.4 ± 0.6%. The only isoprenoid quinone was menaquinone 6 (MK-6). The major fatty acids were iso-C15:0, C15:1-iso G, and C17:0 iso 3-OH. Strain CAU 1569T contained diphosphatidylglycerol, aminoglycolipid, unidentified aminolipid, and three unidentified lipids. Based on phylogenetic, genomic, physiologic, and chemotaxonomic characterizations, strain CAU 1569T represents a novel Snuella species, which the name Snuella sedimenti sp. nov. is proposed. The type of strain is CAU 1569T (= KCTC 82409T = MCCC 1K05670T).


Assuntos
Sedimentos Geológicos , Água do Mar , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Flavobacteriaceae , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2
17.
Arch Microbiol ; 203(9): 5675-5681, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34463811

RESUMO

A novel bacterium, designated CAU 1574T, was isolated from marine sand. Cells were Gram stain negative, aerobic, gliding and rod shaped. Growth was observed at 20-37 °C (optimum, 30 °C), a pH of 5.5-10.0 (optimum, 8.0), and 0-3.0% (w/v) NaCl concentrations (optimum, 1%). Based on the results of 16S rRNA gene sequence analyses, strain CAU 1574T belonged to the genus Echinicola, and showed the highest similarity to Echinicola shivajiensis JCM 17847T (97.5%). Phylogenomic analysis based on consisting of 92 core genes extracted from the genome sequences showed that strain CAU 1574T was affiliated with species in the genus Echinicola. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between strain CAU 1574T and the closely related species were below the cut-off values of 95-96, 90, and 70%, respectively used for species demarcation. The chemotaxonomic data of CAU 1574T were as follows: major isoprenoid quinone, MK-7; predominant polar lipids, phosphatidylethanolamine, two unidentified aminophospholipids and two unidentified lipids; major fatty acids, iso-C15:0, summed feature 3 (C16:1 ω6c/C16:1 ω7c). The 5.4 Mb genome included 20 contigs and 4237 protein-coding genes with a 39.8 mol% G + C content. Based on the phylogenetic, phenotypic, and physiological result, strain CAU 1574T represents a novel species of this genus Echinicola, for which the name Echinicola arenosa sp. nov. is proposed. The type strain is CAU 1574T (= KCTC 82410T = MCCC 1K05669T).


Assuntos
Ácidos Graxos , Areia , Técnicas de Tipagem Bacteriana , Bacteroidetes , Composição de Bases , DNA Bacteriano/genética , Hibridização de Ácido Nucleico , Fosfolipídeos , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
18.
Artigo em Inglês | MEDLINE | ID: mdl-34292146

RESUMO

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain (CAU 1508T) was isolated from marine sediment collected in the Republic of Korea. Growth was observed at 10-45 °C (optimum, 30 °C), pH 4.0-11.0 (optimum, pH 6.0-8.0) and with 0-8.0 % (w/v) NaCl (optimum, 2-4 %). The isolate formed a monophyletic clade in the phylogenetic analyses using 16S rRNA gene and whole-genome sequences, exhibiting the highest similarity to Chachezhania antarctica SM1703T (96.5 %), and representing a distinct branch within the genus Chachezhania (family Rhodobacteraceae). Its whole genome sequence was 5.59 Mb long, with a G+C content of 65.7 mol% and 2183 predicted genes belonging to six functional categories. The average nucleotide identity and digital DNA-DNA hybridization values between CAU 1508T and C. antarctica SM1703T were 79.1 and 22.2 %, respectively. The predominant cellular fatty acids were C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two unidentified phospholipids and one unidentified aminophospholipid. The sole isoprenoid quinone was ubiquinone 10. Phenotypic phylogenetic properties supported the classification of CAU 1508T as representing a novel species of the genus Chachezhania, with the proposed name Chachezhania sediminis sp. nov. The type strain is CAU 1508T (=KCTC 62999T=NBRC 113697T).


Assuntos
Sedimentos Geológicos/microbiologia , Filogenia , Rhodobacteraceae/classificação , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
19.
Arch Microbiol ; 203(5): 2445-2451, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33666688

RESUMO

A Gram-positive, aerobic, endospore-forming, rod-shaped bacterial strain, CAU 1483 T, was isolated from tidal-flat mud in the Republic of Korea. It grew optimally at 30 °C, in a pH 7.0 medium with 2% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAU 1483 T formed a separate clade within Paenibacillaceae together with members of the genus Cohnella. Strain CAU 1483 T exhibited the highest 16S rRNA gene sequence similarity (97.1%) to C. candidum 18JY8-7 T. Whole genome of strain CAU 1483 T was 4.29 Mb in size with a 53.7 mol% G + C content, and included 4046 coding sequences and included 4046 coding sequences, some of which associated with stress response. The average nucleotide identity and digital DNA-DNA hybridization similarity between strain CAU 1483 T and related members of the genus Cohnella were 71.8-74.9% and 22.6-33.9%, respectively. The major respiratory quinone present in this strain was menaquinone-7. Strain CAU 1483 T contained anteiso-C15:0 and iso-C16:0 as the major fatty acids, while its polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysyl-phosphatidylglycerol, phosphatidylcholine, three unidentified aminophospholipids, two unidentified lipids and an unidentified phospholipid. Peptidoglycan type was A1γ meso-Dpm. On the basis of taxonomic characterization, strain CAU 1483 T constitutes a novel species, for which the name Cohnella pontilimi sp. nov. is proposed. The type strain of this novel species is CAU 1483 T (= KCTC 43047 T = NBRC 113953 T).


Assuntos
Bacillales/classificação , Sedimentos Geológicos/microbiologia , Bacillales/química , Bacillales/fisiologia , DNA Bacteriano/genética , Ácidos Graxos/química , Genoma Bacteriano/genética , Peptidoglicano/química , Fosfolipídeos/química , Filogenia , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados
20.
Arch Microbiol ; 203(2): 817-822, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33057911

RESUMO

A Gram-stain-negative, slightly curved, rod-shaped bacterial strain CAU 1517T was isolated from marine sediment in Busan, the Republic of Korea. The taxonomic position of strain CAU 1517T was investigated via a polyphasic approach comprising phenotypic, chemotaxonomic and phylogenetic properties. Strain CAU 1517T grew optimally at 30 °C, pH 7.5 and in the presence of 7% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain CAU 1517T belongs to the genus Halarcobacter and is most closely related to Halarcobacter bivalviorum LMG 26154T (96.5% similarity). The average nucleotide identity and digital DNA-DNA hybridization values between strain CAU 1517T and members of genus Halarcobacter ranged from were 76.7-78.0% and 19.5-21.2%, respectively. The strain contained menaquinone-6 (MK-6) as the only respiratory quinone, and C16:0, summed feature 3 (C16:1 ω7c/C16:1 ω6c), and summed feature 8 (C18:1ω7c/C18:1ω6c) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified aminophospholipids. The G+C content was 28.2 mol%. Therefore, it has been demonstrated that the isolate represents a novel species of the genus Halarcobacter, for which the name Halarcobacter arenosus sp. nov., is proposed. The type strain is CAU 1517T (=KCTC 72232T =NBRC 113955T).


Assuntos
Campylobacteraceae/classificação , Sedimentos Geológicos/microbiologia , Arcobacter/classificação , Arcobacter/genética , Campylobacteraceae/genética , Filogenia , RNA Ribossômico 16S/genética , República da Coreia , Especificidade da Espécie
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