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Methods Mol Biol ; 1980: 121-151, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-30838603


Life-cycle assessment (LCA) is one of the most attractive tools employed nowadays by environmental policy-makers as well as business decision-makers to ensure environmentally sustainable production/consumption of various goods/services. LCA is a systematic, rigorous, and standardized approach aimed at quantifying resources consumed/depleted, pollutants released, and the related environmental and health impacts through the course of consumption and production of goods/service. Algal fuels are no exception and their environmental sustainability could be well scrutinized using the LCA methodology. In line with that, this chapter is devoted to present guidelines on the technical aspects of LCA application in algal fuels while elaborating on major standards used, i.e., ISO 14040 and 14044 standards. Overall, LCA practitioners as well as technical experts dealing with algal fuels in both the public and private sectors could be the main target audience for these guidelines.

Conservação dos Recursos Naturais , Microbiologia Ambiental , Estágios do Ciclo de Vida , Energia Renovável , Biocombustíveis , Mineração de Dados , Bases de Dados Factuais , Humanos , Modelos Teóricos , Software
Data Brief ; 28: 104933, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31886362


In order to develop a product sustainably, multiple analyses, including comprehensive environmental assessment, are required. Solar-assisted production of walnut husk methanolic extract (WHME) as a natural antioxidant for biodiesel was scrutinized by using the life cycle assessment (LCA) approach. More specifically, the environmental sustainability of WHME antioxidant was evaluated and compared to that of propyl gallate (PG), the most widely used synthetic biodiesel antioxidant, under two scenarios. Additionally, supplementary files including the inventory data consisting of raw data as well as elementary flows, mid-point, and end-point categories are presented. The analysis of scenarios revealed that the use of the natural antioxidant and the avoidance of the chemical antioxidant in biodiesel fuel could be regarded as an eco-friendly approach substantially enhancing the environmental friendliness of biodiesel in particular in terms of human health. Furthermore, given the waste-oriented nature of WHME, the scenario involved its application could serve as a promising strategy to simultaneously valorize the agro-waste and generate a value-added product; a move toward implementing the circular economy approach in the biodiesel industry.

Biomed Res Int ; 2015: 597198, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26146623


Exploitation of renewable sources of energy such as algal biodiesel could turn energy supplies problem around. Studies on a locally isolated strain of Dunaliella sp. showed that the mean lipid content in cultures enriched by 200 mg L(-1) myoinositol was raised by around 33% (1.5 times higher than the control). Similarly, higher lipid productivity values were achieved in cultures treated by 100 and 200 mg L(-1) myoinositol. Fluorometry analyses (microplate fluorescence and flow cytometry) revealed increased oil accumulation in the Nile red-stained algal samples. Moreover, it was predicted that biodiesel produced from myoinositol-treated cells possessed improved oxidative stability, cetane number, and cloud point values. From the genomic point of view, real-time analyses revealed that myoinositol negatively influenced transcript abundance of AccD gene (one of the key genes involved in lipid production pathway) due to feedback inhibition and that its positive effect must have been exerted through other genes. The findings of the current research are not to interprete that myoinositol supplementation could answer all the challenges faced in microalgal biodiesel production but instead to show that "there is a there there" for biochemical modulation strategies, which we achieved, increased algal oil quantity and enhanced resultant biodiesel quality.

Biocombustíveis , Biotecnologia , Metabolismo dos Lipídeos/genética , Microalgas/metabolismo , Biomassa , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Humanos , Lipídeos/química , Microalgas/genética , Transdução de Sinais/genética
Appl Biochem Biotechnol ; 175(4): 1817-42, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25427594


Nanobiocatalysis is a new frontier of emerging nanosized material support in enzyme immobilization application. This paper is about a comprehensive review on cellulose nanofibers (CNF), including their structure, surface modification, chemical coupling for enzyme immobilization, and potential applications. The CNF surface consists of mainly -OH functional group that can be directly interacted weakly with enzyme, and its binding can be improved by surface modification and interaction of chemical coupling that forms a strong and stable covalent immobilization of enzyme. The knowledge of covalent interaction for enzyme immobilization is important to provide more efficient interaction between CNF support and enzyme molecule. Enzyme immobilization onto CNF is having potential for improving enzymatic performance and production yield, as well as contributing toward green technology and sustainable sources.

Celulose/química , Enzimas Imobilizadas/química , Nanofibras/química , Adsorção , Reagentes de Ligações Cruzadas/química , Química Verde , Nanofibras/ultraestrutura , Propriedades de Superfície
Bioresour Technol ; 169: 428-438, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25079208


The effect of different aeration rates on the organic matter (OM) degradation during the active phase of oil palm empty fruit bunch (EFB)-rabbit manure co-composting process under constant forced-aeration system has been studied. Four different aeration rates, 0.13 L min(-1) kg(DM)(-1),0.26 L min(-1) kg(DM)(-1),0.49 L min(-1) kg(DM)(-1) and 0.74 L min(-1) kg(DM)(-1) were applied. 0.26 L min(-1) kg(DM)(-1) provided enough oxygen level (10%) for the rest of composting period, showing 40.5% of OM reduction that is better than other aeration rates. A dynamic mathematical model describing OM degradation, based on the ratio between OM content and initial OM content with correction functions of moisture content, free air space, oxygen and temperature has been proposed.

Arecaceae/química , Frutas/química , Modelos Teóricos , Aerobiose , Animais , Biodegradação Ambiental , Carbono/análise , Condutividade Elétrica , Umidade , Concentração de Íons de Hidrogênio , Cinética , Nitrogênio/análise , Compostos Orgânicos/isolamento & purificação , Oxigênio , Coelhos , Solo/química , Temperatura , Termogravimetria
Electron. j. biotechnol ; 12(3): 12-13, July 2009. ilus, tab
Artigo em Inglês | LILACS | ID: lil-551890


16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens, and a probe for bacteria. In situ hybridization with methanogens and bacterial probes and denatured gradient gel electrophoresis within activated sludge clearly confirmed the presence of Methanosaeta sp. and Methanosarcina sp. cells. Methanosaeta concilii was found to be the dominant species in the bioreactor. These results revealed the presence of possibly new strain of Methanosaeta in the bioreactor for treating palm oil mill effluent called Methanosaeta concilii SamaliEB (Gene bank accession number: EU580025). In addition, fluorescent hybridization pictured the close association between the methanogens and bacteria and that the number of methanogens was greater than the number of bacteria.

Óleo de Palmeira/análise , Clonagem Molecular , Digestão Anaeróbia/análise , Genes de RNAr , Methanosarcina/isolamento & purificação , Methanosarcinales/isolamento & purificação , Óleo de Palmeira , Tanques Imhoff/análise , Hibridização in Situ Fluorescente , Reação em Cadeia da Polimerase/métodos