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1.
Front Immunol ; 13: 882796, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35515004

RESUMO

Duck plague (DP) is an acute infectious disease in the duck industry. The duck plague virus (DPV) is the pathogen, a subfamily of alphaherpesvirinae. gE is a type I membrane protein that contains three parts: an extracellular domain, a transmembrane domain, and a cytoplasmic domain. gE is the major virulence determinant of α-herpesvirus. However, the functions of the gE extracellular and cytoplasmic domains have not been reported in DPV. In this study, a gE extracellular domain deletion mutant and a gE cytoplasmic domain deletion mutant were constructed from DPV. Virus replication kinetics showed that the growth titers of both the gE ectodomain-deleted mutant virus and the gE cytoplasmic domain-deleted virus in DEFs were lower than that of the parental virus CHv-50. DPV CHv-gEΔET and DPV CHv-gEΔCT were continuously passed to the 20th passage in DEFs and the 10th in ducklings. The mutant virus DNA after passage was extracted for identification. The results showed that the gE ectodomain and gE cytoplasmic domain deletion mutant viruses have good genetic stability. The ducklings in each group (n=10) were inoculated with the same titers of DPV CHv-gEΔET, DPV CHv-gEΔCT, DPV CHv-ΔgE, and parental CHv-50, respectively. Clinical symptoms and serum antibody levels were detected after inoculation. The results showed that the virulence of DPV CHv-gEΔCT to ducklings was reduced compared with parental CHv-50, while the virulence of DPV CHv-gEΔET to ducklings was significantly reduced. 105 TCID50 DPV CHv-gEΔET or DPV CHv-ΔgE can induce ducklings to produce DPV-specific antibodies, protect the ducklings from virulent CHv challenge. Therefore, DPV CHv-gEΔET may serve as a promising vaccine candidate to prevent and control duck plague.


Assuntos
Alphaherpesvirinae , Infecções por Herpesviridae , Mardivirus , Alphaherpesvirinae/genética , Animais , Patos
2.
Pest Manag Sci ; 2022 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-35567371

RESUMO

BACKGROUND: Paeonol is extracted and isolated as a rich and sustainable natural bioresource from the root bark of Paeonia suffruticosa, the derivatives of which exhibit numerous biological activities. It is well known that ester compounds play a very important role in pest control, such as organophosphorus, carbamate and pyrethroid pesticides. RESULTS: To discover biorational natural product-based pesticides, three series of (60) paeonol ester derivatives (7a-t, 8g,p, 9g,p, 10g-j,n-u, 11g,u, 12g,u, 13a-p, 14b,c, and 15b,c) were prepared by structural modification of paeonol, and their structures were well characterized by 1 H NMR, 13 C NMR, HRMS, and melting point. Furthermore, we assessed the compounds as insecticidal, nematicidal, and anti-oomycete agents against three serious agricultural pests, Mythimna separata, Heterodera glycines, and Phytophthora capsici. Among all tested compounds, 1) Compound 8p showed more significant insecticidal activity than toosendanin, and the final mortality rates of 8p and toosendanin against M. separata (1 mg/mL) were 70.4%, and 51.9%, respectively; 2) Compound 7a exhibited more promising nematicidal activity than paeonol, and the LC50 values of 7a and 1 against H. glycines were 15.47 and 50.80 mg/L, respectively; 3) Compounds 7n and 13m displayed more significant anti-oomycete activity compared to zoxamide against P. capsici, and the EC50 values of 7n, 13m, and zoxamide were 23.72, 24.51, and 26.87 mg/L, respectively; and the protective effect of the compounds against P. capsici in vivo further confirmed the effectiveness of the agents. CONCLUSION: This study suggested that the introduction of a nitro at the C5 or C3 position of paeonol could improve its bioactivity against M. separata, H. glycines, and P. capsici. This article is protected by copyright. All rights reserved.

3.
NPJ Vaccines ; 7(1): 51, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35550523

RESUMO

Live-attenuated vaccines (LAVs) represent a promising approach for flavivirus vaccine development. In the present study, we demonstrated a method for generating flavivirus LAVs based on breaking spatially and temporally regulated C-prM cleavage to disturb the viral assembly process, using an avian flavivirus (Tembusu virus) as the model. Using reverse genetics technology, we successfully generated two recombinant viruses (CQW1-IRES-mC and CQW1-MINI-mC) with bicistronic genomic RNA in which native capsid genes were deleted and instead expressed in the 3'UTR under the control of an internal ribosome entry site (IRES) or minimum IRES. Both viruses showed a significantly attenuated phenotype in vitro due to impaired viral assembly, and the engineered mutations were genetically stable in vitro within ten passages. Importantly, their virulence was also highly attenuated in ducklings and suckling mice and did not cause any overt clinical symptoms or mortality. In addition, a single dose of immunization with any of these mutant viruses could completely protect ducklings from a lethal challenge, and no viremia was detected after immunization and challenge, even though the viruses induced a relatively moderate immune response in terms of the T-lymphocytes proliferative response and the level of neutralization antibodies compared with that obtained with the wild-type virus. Besides, a recombinant virus ectopically expressing the prM-E protein was also generated in the present study, but this virus was too attenuated with severely decreased proliferation. Our results indicated that the use of a recombinant flavivirus that ectopically expresses structural proteins could be an effective and universal method for flavivirus LAVs development.

4.
JVS Vasc Sci ; 3: 211-218, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35574516

RESUMO

Objective: Fluorescence contrast technology using indocyanine green (ICG) could be useful for the rapid, dynamic, and objective assessment of blood vessels and the surrounding tissues when combined with near-infrared (NIR) imaging. Although ICG is a clinically available NIR fluorescence imaging probe, it can easily aggregate and is, thus, unstable. In the present study, we examined the efficacy of a host-guest ICG-ß-cyclodextrin (CD) complex, which is used in pharmaceutics to improve the water solubility, stability, and bioavailability of hydrophobic molecules, for NIR imaging after hind footpad administration in a rat model. Methods: To verify the performance of the ICG-ß-CD complex with the host-guest self-assembly method in vivo, we performed simultaneous small animal (IVIS Spectrum system; PerkinElmer, Waltham, MA) and clinical (DIGI-MIH-001 near-infrared fluorescence imaging system; Beijing Digital Precision Medical Technology Co, Ltd, Beijing, China) imaging and evaluated the fluorescent properties of the ICG-ß-CD complex in the hind footpad model of Sprague-Dawley male rats. Results: We successfully prepared the ICG-ß-CD complex. Compared with ICG, in vivo experiments showed that this complex had reduced absorbance at 710 nm and increased absorbance at 780 nm, indicating that it could prevent the dimeric aggregation of ICG, and a significantly higher fluorescence intensity at 730 nm excitation. After injection of 1.25 mg/mL of ICG or ICG-ß-CD complex solutions into the rat hind footpad, fluorescent NIR lymphatic images were observed with both imaging systems. During the 12-hour observation period, the signal background ratio of ICG-ß-CD showed a greater acute increase and a higher signal background ratio compared with ICG. The signal background ratio of ICG-ß-CD was 125 to 100 from 260 to 540 minutes. These in vivo data suggest that ICG-ß-CD has greater diffusion from the injection site and faster transport to the lymphatic system compared with ICG. Conclusions: ICG-ß-CD showed faster lymphatic transport than ICG, allowing for more rapid lymphatic NIR imaging. Thus, the ICG-ß-CD complex might be a promising fluorescent agent for clinical lymphatic NIR imaging.

5.
Aesthetic Plast Surg ; 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35441848

RESUMO

BACKGROUND: Polyacrylamide hydrogel (PAAG) has been used globally for breast augmentation, leading to long-term clinical complications. However, whether the infiltrated fibrotic capsule should be removed with PAAG to alleviate the complications remains unclear. This study aimed to ascertain different causes of complications and proper management strategies for PAAG removal in augmented breasts. METHODS: From July 2015 to December 2019, patients who underwent breast augmentation with PAAG and in whom surgical intervention was undertaken for PAAG-associated adverse events at Shanghai Ninth People's Hospital were retrospectively reviewed. Patients were categorized into two groups according to whether the fibrotic capsule was removed (RFC) or not (NRFC). Aesthetic outcomes, PAAG residues, and adverse events were evaluated post-operatively to assess whether important issues pertaining to these arose following fibrotic capsule removal. Tissue histology and PAAG degradation analysis were implemented to investigate immune response, degradability, and toxicity of PAAG. RESULTS: Altogether, 257 patients (88 RFC and 169 NRFC patients) were enrolled. 73.4% and 79.5% of the RFC and NRFC groups showed fairly good outcomes, with no significant difference, respectively. (X2 = 0.0804, p = 0.79) Significant differences were found between two surgical techniques upon patient satisfaction, respectively. (X2 = 3.529; p = 0.0301). Predictor of poor outcomes identified scar (OR, 4.555, p = 0.0019) and PAAG residue (OR, 5.379, p = 0.0003). Predictor of patient satisfaction identified post-operative outcomes (OR, 3.797; 95% CI, 1.860-8.923; p = 0.0002) and surgical technique (NRFC) (OR, 2.519; 95% CI, 1.449-4.434; p = 0.0008). CONCLUSIONS: Both treatment strategies showed good results in our study. Removal of the fibrotic capsule from infiltration of PAAG largely depends on the individual psychological condition, aesthetic expectations, complications, and magnetic resonance imaging results. While PAAG does not degrade in the host's body over time, it may elicit immune reactions and chronic inflammation in the long term. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine Ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266.

6.
Nat Commun ; 13(1): 1802, 2022 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-35379821

RESUMO

The structural transformations of metal nanoclusters are typically quite complex processes involving the formation and breakage of several bonds, and thus are challenging to study. Herein, we report a case where two lacunary Keggin polyoxometallate templated silver single-pods [PW9O34@Ag51] (SD/Ag51b) fuse to a double-pod [(PW9O34)2@Ag72] by reacting with 4,4'-bipyridine (bipy) or 1,4-bis(4-pyridinylmethyl)piperazine (pi-bipy). Their crystal structures reveal the formation of a 2D 44-sql layer (SD/Ag72a) with bipy and a 3D pcu framework (SD/Ag72c) with pi-bipy. The PW9O349- retains its structure during the cluster fusion and cluster-based network formation. Although the two processes, stripping of an Ag-ligands interface followed by fusion, and polymerization, are difficult to envisage, electrospray ionization mass spectrometry provides enough evidences for such a proposal to be made. Through this example, we expect the structural transformation to become a powerful method for synthesizing silver nanoclusters and their infinite networks, and to evolve from trial-and-error to rational.

7.
Vet Microbiol ; 269: 109433, 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35489297

RESUMO

Flavivirus nonstructural protein 5 (NS5) harbors the N-terminal methyltransferase (MTase) and C-terminal polymerase RNA-dependent RNA polymerase (RdRp). The intramolecular NS5 features an integral MTase and RdRp interface with two components: a six-residue hydrophobic network and a GTR linker. Herein, the determinants of the MTase-RdRp interface and flavivirus substituted GTR linker were explored in TMUV replication and proliferation. First, the NanoLuc® Binary Technology (NanoBiT) and coimmunoprecipitation assays (Co-IP) methods confirmed the interaction between the MTase and RdRp domains of TMUV NS5. To screen for an optimal orientation for reporter gene fusion to the protein of interest, the signal activity of eight combinations of MTase and RdRp was explored. Intriguingly, all the combinations with the reporter gene fused to the C-terminal of MTase (1.1 C/2.1 C MTase) could barely detect any positive signal, suggesting a role for the GTR linker of the MTase C-terminal in MTase-RdRp affinity. Based on the flavivirus NS5 homologous interplay, we introduced alanine mutations into the MTase-RdRp interface of TMUV NS5. However, no single or pairwise mutation was found to abort the NS5 intramolecular interaction. Then, a mutated replicon and infectious clone were constructed to analyze the replication ability and properties of the recombinant virus. The mutant replicons of MTase F113A and M115A replicated to comparable extent as the wild type (WT). However, the replication level of the mutant MTase W121A was impaired without an obvious decrease in proliferation and virulence. Both the RdRp F351A and P585A mutants could replicate and proliferate well. Notably, the RdRp F467A virus was attenuated and did not strikingly impair the MTase-RdRp interaction. Furthermore, the TMUV was specifically compatible with the substituted NS5 with a Japanese encephalitis virus (JEV) GTR linker. Compensatory mutations were observed in the context of a defective MTase-RdRp interface after several passages of the rescued mutants in BHK-21 cells. A greater understanding of the molecular mechanism of the NS5 protein controlling duck TMUV replication will facilitate the design of novel therapies.

8.
Comput Intell Neurosci ; 2022: 5509129, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35432518

RESUMO

Dermatofibrosarcoma protuberans (DFSP) is a type of skin cancer that is extremely rare. Its standard treatment is either surgical wide-local excision (WLE) or Mohs micrographic surgery (MMS). Which method has the lowest recurrence rate is unknown. Dermatofibrosarcoma protuberans is an uncommon soft tissue sarcoma with a high propensity for recurrence. It has always remained a clinical challenge. More technology is needed to treat the disease. We reviewed our cases and published experience and evaluated whether 3D modeling could precisely define tumor morphological characteristics and assist excision in slow Mohs surgery. There were 18 dermatofibrosarcoma protuberan cases enrolled. They were treated in Shanghai Ninth People's Hospital from 2014 to 2019. All the 18 included patients presented with primary disease and no metastasis. All subjects had undergone thorough imaging examinations including CT and MRI. The 3D tumor reconstruction models were created for their tumors. We precisely estimated tumor boundaries and sizes according to those 3D models. Afterward, patients underwent slow Mohs surgery and surgical repair of tissue defects following tumor resection. The 3D tumor reconstruction models were successfully established. The predicted tumor volumes were measured in all 18 cases. The average volume was 38.5 cm3 (range: 8.4 cm3-183.6 cm3), which allowed for accurately locating the tumor. Tumors were completely removed in one stage of slow MMS surgery. In the second-stage surgery, the defects were repaired by different surgical methods including direct soft tissue closure, skin grafting, local flaps, or free flaps. Most patients experienced no significant complications. This practice indicated that the combination of a 3D reconstruction model and slow Mohs surgery achieves more precise and complete DFSP resection to decrease the recurrence rate.


Assuntos
Dermatofibrossarcoma , Cirurgia de Mohs , China , Dermatofibrossarcoma/patologia , Dermatofibrossarcoma/secundário , Dermatofibrossarcoma/cirurgia , Humanos , Imageamento Tridimensional , Cirurgia de Mohs/métodos , Recidiva Local de Neoplasia/patologia , Recidiva Local de Neoplasia/cirurgia
9.
Poult Sci ; 101(6): 101863, 2022 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-35489250

RESUMO

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.

10.
Fish Shellfish Immunol ; 124: 74-81, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35378307

RESUMO

The interaction between microplastics and contaminants has potentially generated new undefined risks on animals and ecosystems, and nano-microplastics are considered to be more harmful than microplastics. This experiment investigated the interactions and effects of nano-microplastics with heavy metals cadmium in hybrid snakehead. Different concentrations of nano-microplastics 80 nm (50 µg/L and 500 µg/L) and Cd (50 µg/L) were used for exposure, and four sampling points were set for 24 h, 48 h, 96 h and clear-48 h. Results indicated that the morphology of gill was altered under the influence of nano-microplastics and cadmium, and the damage was aggravated with time. Nano-microplastics and Cd can cause oxidative damage to fish liver partly by effect the activities of antioxidant enzyme, and significantly suppressed the expressions of genes related to the inflammation (IL-1ß and TNF-α) and as well as significantly up-regulated the expression of genes HSP70 and SOD. Additionally, the mRNA levels of MT gene can be speculated that the heavy metal cadmium may accumulated in the body over time. And the concentration of heavy metals will also affect their accumulation in the body. Our study elucidated the nano-microplastics and Cd will increase the impact on environmental and organisms that the nano-microplastics contribute to the bioaccumulation of metals, which served as a new support for study the interaction between environmental contaminants.

11.
Nat Commun ; 13(1): 1493, 2022 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-35315431

RESUMO

Nucleotide second messengers, such as cAMP and c-di-GMP, regulate many physiological processes in bacteria, including biofilm formation. There is evidence of cross-talk between pathways mediated by c-di-GMP and those mediated by the cAMP receptor protein (CRP), but the mechanisms are often unclear. Here, we show that cAMP-CRP modulates biofilm maintenance in Shewanella putrefaciens not only via its known effects on gene transcription, but also through direct interaction with a putative c-di-GMP effector on the inner membrane, BpfD. Binding of cAMP-CRP to BpfD enhances the known interaction of BpfD with protease BpfG, which prevents proteolytic processing and release of a cell surface-associated adhesin, BpfA, thus contributing to biofilm maintenance. Our results provide evidence of cross-talk between cAMP and c-di-GMP pathways through direct interaction of their effectors, and indicate that cAMP-CRP can play regulatory roles at the post-translational level.


Assuntos
Regulação Bacteriana da Expressão Gênica , Transdução de Sinais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biofilmes , Proteína Receptora de AMP Cíclico/genética , GMP Cíclico/análogos & derivados , GMP Cíclico/metabolismo , Transdução de Sinais/genética
12.
Vet Microbiol ; 267: 109396, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35279430

RESUMO

Although the role of the flavivirus non-structural 2B (NS2B) as a viral protease cofactor has been investigated, its role in viral proliferation has rarely been studied. Moreover, important details on the involvement of the flavivirus NS2B in the virus replication cycle, including attachment, entry, RNA replication, assembly and release, remain unknown. Here, an alignment of flavivirus NS2B was performed to select conserved amino acids for the site-directed mutagenesis study. G92, which is not totally conserved to mutation, was also used. Certain mutations (P32A, G36A and G37A) completely blocked viral RNA synthesis, four mutations (D25A, A43Y, V44A, G92A and P112A) showed a slight defect in virus replication, and only the G92A mutant affected virus proliferation by blocking virus release but not RNA replication. By taking advantage of the tembusu virus (TMUV) replicon that represents viral genome RNA replication, we showed that the G92A mutation did not obviously impair viral replication. Naturally, the NS2B3 G92A mutation protein did not affect the self-cleavage ability or NS2B3 protein-mediated cleavage of the NS2AB G92A mutant. These results indicated that the G92A mutation did not affect viral protease activity. By using the TMUV infection clone and the replicon package system, the effect of G92 on the virus lifecycle was narrowed to viral resemblance or release. Subcellular fractionation further confirmed that G92A attenuated TMUV by reducing virus assembly. Collectively, these studies extend the list of NS2B functions beyond protease activity as a cofactor, show that transmembrane region amino acids play an important role in viral replication and indicate that G92 is involved in the assembly of infectious TMUV particles.


Assuntos
Flavivirus , Proteínas não Estruturais Virais , Animais , Flavivirus/genética , Proteínas não Estruturais Virais/metabolismo , Montagem de Vírus/genética , Replicação Viral/genética
13.
Angew Chem Int Ed Engl ; 61(20): e202200823, 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35229421

RESUMO

Stepwise assembly starting from a preassembled metalloligand is a promising approach to obtain otherwise unattainable silver nanoclusters, but hard to be intrinsically identified due to the lack of convincing evidence to justify such a process. Herein, hexagonal and rectangular Ag18 nanoclusters are constructed from the [Mo2 O5 (PTC4A)2 ]6- (H4 PTC4A=p-phenyl-thiacalix[4]arene) metalloligand through stepwise assembly. The formation of the metalloligand is confirmed by electrospray ionization mass spectrometry, then assembled with silver ions to form two geometrically different Ag18 nanoclusters in different solvents. The cyclization from the metalloligand to [(Mo2 O5 PTC4A)6 ]12- can be realized without alcohols and otherwise blocked by them. The installation of this metalloligand not only provides comprehensive understanding of how the solvents regulate the silver nanocluster structures, but also brings new insights for the controllable ligand metallization and subsequent condensation.

14.
Vet Res ; 53(1): 17, 2022 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-35236414

RESUMO

Pasteurella multocida infection frequently causes fowl cholera outbreaks, leading to huge economic losses to the poultry industry worldwide. This study developed a novel live attenuated P. multocida vaccine strain for ducks named PMZ2 with deletion of the gatA gene and first four bases of the hptE gene, both of which are required for the synthesis of the lipopolysaccharide (LPS) outer core. PMZ2 produced a truncated LPS phenotype and was highly attenuated in ducks with a > 105-fold higher LD50 than the wild-type strain. PMZ2 colonized the blood and organs, including the spleen, liver and lung, at remarkably reduced levels, and its high dose of oral infection did not cause adverse effects on body temperatures and body weights in ducks. To evaluate the vaccine efficacy of the mutant, ducklings were inoculated orally or intranasally with PMZ2 or PBS twice and subsequently subjected to a lethal challenge. Compared with the PBS control, PMZ2 immunization stimulated significantly elevated serum IgG, bile IgA and tracheal IgA responses, especially after the boost immunization in both the oral and intranasal groups, and the induced serum had significant bactericidal effects against the wild-type strain. Furthermore, the two PMZ2 immunization groups exhibited alleviated tissue lesions and significantly decreased bacterial loads in the blood and organs compared with the PBS group post-challenge. All the ducks in the PMZ2 oral and intranasal groups survived the challenge, while 70% of ducks in the PBS group succumbed to the challenge. Thus, the P. multocida mutant with mutation of the gatA gene and part of the hptE gene proved to be an effective live attenuated vaccine candidate for prevention of fowl cholera in ducks.


Assuntos
Infecções por Pasteurella , Pasteurella multocida , Doenças das Aves Domésticas , Animais , Vacinas Bacterianas , Patos , Lipopolissacarídeos , Infecções por Pasteurella/microbiologia , Infecções por Pasteurella/prevenção & controle , Infecções por Pasteurella/veterinária , Pasteurella multocida/genética , Doenças das Aves Domésticas/microbiologia
15.
Appl Spectrosc ; : 37028221092474, 2022 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-35315296

RESUMO

In optical noninvasive glucose detection, how to detect the glucose-caused signals from the constant human variations and disturbed probing conditions is always the biggest challenge. Developing effective measurement strategies is essential to realize the detection. A NIR spectroscopy-based strategy is studied to effectively solve the in vivo measurement issues, obtaining clean blood glucose-caused signals. Two solutions composing our strategy are applied to the NIR spectroscopy-based measurement system to acquire clean raw signals in the data collection. Which are: a customized high signal-to-noise ratio multi-ring InGaAs detector is introduced to reduce the influences of human variations; a fixing and aiming method are introduced to reproduce a consistent measurement condition. 17 cases of glucose tolerance test (GTT) on healthy and diabetic volunteers are conducted to validate the strategy. Human experiment results clearly show that the expected blood glucose changes have been detected at 1550 nm. The average correlation coefficient of the 17 cases of GTT between light signal and glucose reference reaches 0.84. The proposed measurement strategy is verified feasible for the glucose detecting in vivo. The strategy provides references to further studies and product developments for the NIR spectroscopy-based glucose measurement, and references to other optical measurements in vivo.

16.
BMC Palliat Care ; 21(1): 30, 2022 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-35255876

RESUMO

BACKGROUND: Anticipatory grief has been shown to be highly prevalent among family caregivers of patients with advanced illness. Qualitative study suggests that illness uncertainty may be one of the core characteristics of anticipatory grief, but it has not been confirmed in quantitative studies. Therefore, the purpose of this study was to explore the relationship between anticipatory grief and illness uncertainty among Chinese family caregivers of patients with advanced lung cancer and to determine the factors influencing anticipatory grief. METHODS: This descriptive cross-sectional study used a convenience sampling method and recruited 254 inpatient family caregivers from the thoracic medicine ward of Liaoning Cancer Hospital & Institute in Shenyang, mainland China. Anticipatory grief (Anticipatory Grief Scale (AGS), illness uncertainty (Uncertainty in Illness Scale Family Caregiver Version) and sociodemographic information (Self-compiled general information questionnaire) were measured using validated self-report measures. RESULTS: Chinese family caregivers of patients with advanced lung cancer had high levels of anticipatory grief (73.5 ± 16.1). The results of the correlation analysis showed a positive association between anticipatory grief and illness uncertainty (r = 0.580, P < 0.001). The final linear regression model with anticipatory grief as the dependent variable included four variables: illness uncertainty (ß = 0.674, P < 0.001), lack of informativeness (ß = - 0.168, P = 0.08), monthly income (ß = 0.139, P = 0.006), and caregiving burden (ß = - 0.196, P < 0.001). CONCLUSIONS: Illness uncertainty is probably an important factor affecting anticipatory grief. Excessive caregiving burden is associated with high levels of anticipatory grief. Improving illness uncertainty and caregiving burden may effectively reduce anticipatory grief among Chinese family caregivers.


Assuntos
Cuidadores , Neoplasias Pulmonares , Estudos Transversais , Pesar , Humanos , Inquéritos e Questionários , Incerteza
17.
Vet Res ; 53(1): 22, 2022 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-35303942

RESUMO

Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) are cytosolic pattern recognition receptors that initiate innate antiviral immunity. Recent reports found that duck RLRs significantly restrict duck plague virus (DPV) infection. However, the molecular mechanism by which DPV evades immune responses is unknown. In this study, we first found that the DPV UL41 protein inhibited duck interferon-ß (IFN-ß) production mediated by RIG-I and melanoma differentiation-associated gene 5 (MDA5) by broadly downregulating the mRNA levels of important adaptor molecules, such as RIG-I, MDA5, mitochondrial antiviral signalling protein (MAVS), stimulator of interferon gene (STING), TANK-binding kinase 1 (TBK1), and interferon regulatory factor (IRF) 7. The conserved sites of the UL41 protein, E229, D231, and D232, were responsible for this activity. Furthermore, the DPV CHv-BAC-ΔUL41 mutant virus induced more duck IFN-ß and IFN-stimulated genes (Mx, OASL) production in duck embryo fibroblasts (DEFs) than DPV CHv-BAC parent virus. Our findings provide insights into the molecular mechanism underlying DPV immune evasion.


Assuntos
Patos , Interferon beta , Animais , Imunidade Inata , Interferon beta/genética , Interferons , Estabilidade de RNA
18.
Tomography ; 8(2): 644-656, 2022 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-35314631

RESUMO

This observer study investigates the effect of computerized artificial intelligence (AI)-based decision support system (CDSS-T) on physicians' diagnostic accuracy in assessing bladder cancer treatment response. The performance of 17 observers was evaluated when assessing bladder cancer treatment response without and with CDSS-T using pre- and post-chemotherapy CTU scans in 123 patients having 157 pre- and post-treatment cancer pairs. The impact of cancer case difficulty, observers' clinical experience, institution affiliation, specialty, and the assessment times on the observers' diagnostic performance with and without using CDSS-T were analyzed. It was found that the average performance of the 17 observers was significantly improved (p = 0.002) when aided by the CDSS-T. The cancer case difficulty, institution affiliation, specialty, and the assessment times influenced the observers' performance without CDSS-T. The AI-based decision support system has the potential to improve the diagnostic accuracy in assessing bladder cancer treatment response and result in more consistent performance among all physicians.


Assuntos
Sistemas de Apoio a Decisões Clínicas , Neoplasias da Bexiga Urinária , Inteligência Artificial , Humanos , Tomografia Computadorizada por Raios X , Neoplasias da Bexiga Urinária/diagnóstico por imagem , Neoplasias da Bexiga Urinária/terapia , Urografia
19.
ACS Nano ; 16(3): 4500-4507, 2022 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-35230817

RESUMO

Metalloligand strategy has been well recognized in the syntheses of heterometallic coordination polymers; however, such a strategy used in the assembly of silver nanoclusters is not broadly available. Herein, we report the stepwise syntheses of a family of halogen-templated Ag42 nanoclusters (Ag42c-Ag42f) based on MoVI-anchored p-tert-butylthiacalix[4]arene (H4TC4A) as a metalloligand (hereafter named MoO3-TC4A). X-ray crystallography demonstrates that they are similar C3-symmetric silver-organic nanocalices capped by six MoO3-TC4A metalloligands, which are evenly distributed up and down the base of 42 silver atoms. These nanoclusters can be disassembled to six bowl-shaped [Ag11(MoO3-TC4A)(RS)3] secondary building units (SBUs, R = Et or nPr), which are fused together in a face-sharing fashion surrounding Cl- or Br- as a central anion template. The electrospray mass spectrometry (ESI-MS) indicates their high stabilities in solution and verifies the formation of the MoO3-TC4A metalloligand, thereby rationalizing the overall stepwise assembly process for them. Moreover, Ag42c shows lower cytotoxicity and better activity against the HepG-2 cell line than MCF-7 and BGC-823. These results not only exemplify the effectiveness of a thiacalix[4]arene-based metalloligand in the assembly of silver nanoclusters but also give us profound insight about the step-by-step assembly process in silver nanoclusters.

20.
Inorg Chem ; 61(10): 4251-4256, 2022 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-35238553

RESUMO

Metal-organic frameworks (MOFs) represent a kind of low-energy physisorbent with modifiable pores and framework structures; however, a deep understanding of how these structural features influence properties is a prerequisite for the rational design and development of tailor-made materials for advanced applications. In this report, a MOF, [Ni2(TCPP-Ni)1/4(TPIM)2(COOH)F][(Me2NH2)SiF6]·xS (SDU-CP-1; S = solvent molecules, SDU = Shandong University, and CP = coordination polymer), assembled by tetrakis(4-carboxyphenyl)porphyrin (TCPP-Ni) and 2,4,5-tris(4-pyridyl)imidazole (TPIM) ligands as well as Ni2+ cations is reported. Interestingly, inorganic SiF62- anions do not serve as the pillars like precedents in the framework but are just counterions, which endows SDU-CP-1 with high uptake for C2H2 and adsorption selectivity (2.5-4.2) for C2H2/CO2 at room temperature, as certified by gas adsorption and separation experiments and grand canonical Monte Carlo calculation.

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