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1.
Anticancer Drugs ; 2021 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-34261920

RESUMO

BACKGROUND: Albendazole (ABZ), a clinical antiparasitic drug, has shown potential antitumor effects in various tumors. Herein, we prepared dimeric cRGD [(cRGD)2] modified human serum albumin (HSA) nanosystem to co-delivery of albendazole (ABZ) and iodine-131 (131I) for chemoradiotherapy of triple-negative breast cancer (TNBC). MATERIALS METHODS: HSA@ABZ NPs were synthesized by the self-assembly method. 131I-(cRGD)2/HSA@ABZ NPs were fabricated through covalently binding HSA@ABZ NPs with (cRGD)2 peptides, followed by chloramine T direct labeling with 131I. In vitro therapeutic effects on TNBC (MDA-MB-231 and 4T1 cells) were determined using MTT assay, crystal violet assay, wound-healing assay and western blotting analysis. In vivo treatment was performed using 4T1-bearing mice, and the tumor-targeting efficacy was assessed by gamma imaging. The distribution of NPs was quantitatively analyzed by detecting the gamma counts in tumor and main organs. RESULTS: The nanoparticles possessed negative charge, moderate size and good polydispersity index. Dual responding to pH and redox, the in vitro release rate of ABZ was more than 80% in 72 h. In vitro, NPs inhibited the proliferation of TNBC cells in a concentration-dependent manner and decreased cell migration. Western blotting analysis showed that the NPs, as well as free ABZ, cell-dependently induced autophagy and apoptosis by restraining or promoting the expression of p-p38 and p-JNK MAPK. In vivo, gamma imaging exhibited an earlier and denser radioactivity accumulation in tumor of 131I-(cRGD)2/HSA@ABZ NPs compared to NPs free of (cRGD)2 conjugating. Furthermore, 131I-(cRGD)2/HSA@ABZ NPs significantly suppressed tumor growth by restraining proliferation and promoting apoptosis in vivo. CONCLUSIONS: Our study suggested that the nanoparticles we developed enhanced tumor-targeting of ABZ and increased antitumor effects by combination of chemotherapy and radiotherapy.

2.
Antiviral Res ; : 105141, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34274417

RESUMO

Ebola virus (EBOV) of the genus Ebolavirus belongs to the family Filoviridae, which cause disease in both humans and non-human primates. Zaire Ebola virus accounts for the highest fatality rate, reaching 90%. Considering that EBOV has a high infection and fatality rate, the development of a highly effective vaccine has become a top public health priority. Glycoprotein (GP) plays a critical role during infection and protective immune responses. Herein, we developed an EBOV GP recombinant DNA vaccine that targets the major histocompatibility complex (MHC) class II compartment by fusing with lysosomal-associated membrane protein 1 (LAMP1). Through lysosome trafficking and antigen presentation transferring, the LAMP1 targeting strategy successfully improved both humoral and cellular EBOV-GP-specific immune responses. After three consecutive immunizations, the serum antibody titers, especially the neutralizing activity of mice immunized with the pVAX-LAMP/GPEBO vaccine were significantly higher than those of the other groups. Antigen-specific T cells showed positive activity against three dominant peptides, EAAVSHLTTLATIST, IGEWAFWETKKNLTR, and ELRTFSILNRKAIDF, with high affinity for MHC class II molecules predicted by IEDB-recommended. Preliminary safety observation denied histological alterations. DNA vaccine candidate pVAX-LAMP/GPEBO shows promise against Ebola epidemic and further evaluation is guaranteed.

3.
Sensors (Basel) ; 21(13)2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34282788

RESUMO

Gas-path anomalies account for more than 90% of all civil aero-engine anomalies. It is essential to develop accurate gas-path anomaly detection methods. Therefore, a weakly supervised gas-path anomaly detection method for civil aero-engines based on mapping relationship mining of gas-path parameters and improved density peak clustering is proposed. First, the encoder-decoder, composed of an attention mechanism and a long short-term memory neural network, is used to construct the mapping relationship mining model among gas-path parameters. The predicted values of gas-path parameters under the restriction of mapping relationships are obtained. The deviation degree from the original values to the predicted values is regarded as the feature. To force the extracted features to better reflect the anomalies and make full use of weakly supervised labels, a weakly supervised cross-entropy loss function under extreme class imbalance is deployed. This loss function can be combined with a simple classifier to significantly improve the feature extraction results, in which anomaly samples are more different from normal samples and do not reduce the mining precision. Finally, an anomaly detection method is deployed based on improved density peak clustering and a weakly supervised clustering parameter adjustment strategy. In the improved density peak clustering method, the local density is enhanced by K-nearest neighbors, and the clustering effect is improved by a new outlier threshold determination method and a new outlier treatment method. Through these settings, the accuracy of dividing outliers and clustering can be improved, and the influence of outliers on the clustering process reduced. By introducing weakly supervised label information and automatically iterating according to clustering and anomaly detection results to update the hyperparameter settings, a weakly supervised anomaly detection method without complex parameter adjustment processes can be implemented. The experimental results demonstrate the superiority of the proposed method.

4.
Int J Biol Macromol ; 186: 341-350, 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34252462

RESUMO

As the most abundant aromatic biopolymer, lignin presents great potential to produce valuable materials and chemicals. However, its large-scale value-added application is still facing many practical challenges and one of them is the unstable properties caused by lignin heterogeneity. Herein, we developed a novel "one-step-three-fractions" fractionation strategy to reduce lignin heterogeneity using aqueous two-phase system (ATPS) composed of (NH4)2SO4 and ethanol. In contrast to conventional step-wise fractionation processes, the proposed process subdivided heterogeneous lignin into three homogeneous fractions in only one step: the first fraction (F1) dissolved in the ethanol-rich top layer; the second fraction (F2) dissolved in the salt-rich bottom layer and the last fraction (F3) insoluble in both two layers. F2 presented the lowest molecular weight followed by F1 while F3 showed the highest molecular weight. With the increase of molecular weight, the contents of guaiacyl unit and ß-O-4 linkage increased while the content of hydrophilic groups (carboxyl and aromatic hydroxyl) decreased significantly. Moreover, the ATPS exhibited satisfactory recyclability and the fractionation approach could be applied to different types/sources of lignin. Consequently, the work indicates that ATPS is a novel and effective way to fractionate lignin and reduce its molecular weight polydispersity and structural heterogeneity in one step.

5.
Comput Methods Programs Biomed ; 208: 106271, 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34274612

RESUMO

PET image reconstruction from incomplete data, such as the gap between adjacent detector blocks generally introduces partial projection data loss, is an important and challenging problem in medical imaging. This work proposes an efficient convolutional neural network (CNN) framework, called GapFill-Recon Net, that jointly reconstructs PET images and their associated sinogram data. GapFill-Recon Net including two blocks: the Gap-Filling block first address the sinogram gap and the Image-Recon block maps the filled sinogram onto the final image directly. A total of 43,660 pairs of synthetic 2D PET sinograms with gaps and images generated from the MOBY phantom are utilized for network training, testing and validation. Whole-body mouse Monte Carlo (MC) simulated data are also used for evaluation. The experimental results show that the reconstructed image quality of GapFill-Recon Net outperforms filtered back-projection (FBP) and maximum likelihood expectation maximization (MLEM) in terms of the structural similarity index metric (SSIM), relative root mean squared error (rRMSE), and peak signal-to-noise ratio (PSNR). Moreover, the reconstruction speed is equivalent to that of FBP and was nearly 83 times faster than that of MLEM. In conclusion, compared with the traditional reconstruction algorithm, GapFill-Recon Net achieves relatively optimal performance in image quality and reconstruction speed, which effectively achieves a balance between efficiency and performance.

6.
Cell Death Dis ; 12(8): 717, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34285190

RESUMO

Renal cell carcinoma (RCC) cells have increased lipogenesis and cholesterol synthesis. Sterol regulatory element-binding protein-1 (SREBP1) is cleaved by site 1 protease (S1P) to release the transcriptionally active amino-terminal domain. PF-429242 is a potent and competitive S1P inhibitor. We here tested its activity in RCC cells. In established and primary human RCC cells, PF-429242 potently inhibited cell proliferation, migration, and invasion. The S1P inhibitor provoked apoptosis activation in RCC cells. Furthermore, shRNA-mediated S1P silencing or CRISPR/Cas9-induced S1P knockout led to RCC cell growth inhibition and apoptosis activation. Conversely, ectopic overexpression of SREBP1 or S1P augmented RCC cell proliferation and migration. Daily i.v. injection of a single dose of PF-429242 robustly inhibited RCC xenograft growth in severe combined immunodeficiency mice. Additionally, intratumoral injection of S1P shRNA lentivirus inhibited RCC xenograft growth in mice. SREBP1, S1P, and its target gene low density lipoprotein receptor (LDLR) were significantly elevated in human RCC tissues. These results suggest that targeting S1P by PF-429242 inhibited RCC cell growth in vitro and in vivo.

7.
Pest Manag Sci ; 2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-34223694

RESUMO

BACKGROUND: The striped rice stem borer, Chilo suppressalis (Lepidoptera: Pyraidae), is one of the most serious rice pests in China. Chlorantraniliprole was extensively used for C. suppressalis control in the past ten years, and some field populations have developed high resistance. In this study, we report chlorantraniliprole resistance status of C. suppressalis in China and the resistance mechanism. RESULTS: Significant geographical variations of chlorantraniliprole susceptibility were observed among 28 C. suppressalis field populations in 2019-2020. The LC50 values varied from 2907.874 mg/L (XS19) to 1.524 mg/L (QW19). Most tested field populations collected from Zhejiang, Jiangxi, Hunan and Anhui provinces in 2020 showed high level of resistance to chlorantraniliprole (RR = 311.9-2060.1), while Jiangsu and Sichuan provinces' populations remained susceptible. Analysis of RyR mutations showed that mutations of I4758M, Y4667D, Y4667C and Y4891F were present in resistant populations of C. suppressalis with different levels of chlorantraniliprole resistance. The frequency of Y4667C mutation is correlated to chlorantraniliprole resistance in YY19 (RR = 702.6) and YY20 (RR = 1426.8) populations, with the homozygous mutation frequency of 15.6% and 29.4%, respectively. High contribution of I4758M and Y4667C double mutation to diamide resistance was demonstrated with CRISPR/Cas9 modified D. melanogaster. Flies bearing Y4667C mutation (I4758M and Y4667C double mutation in C. suppressalis) exhibited high resistance to chlorantraniliprole (RR = 172.1), and moderate resistance to cyantraniliprole (RR = 79.2) and tetra chlorantraniliprole (RR = 43.6), which were higher than that of single mutations. CONCLUSIONS: Chlorantraniliprole resistance in C. suppressalis is intensifying in China. RyR double mutations (i.e., I4758M and Y4667C) confer higher diamide resistance than single mutations. This article is protected by copyright. All rights reserved.

8.
Adv Mater ; : e2101741, 2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-34219292

RESUMO

The electroreduction of carbon dioxide (CO2 RR) to CH4 stands as one of the promising paths for resourceful CO2 utilization in meeting the imminent "carbon-neutral" goal of the near future. Yet, limited success has been witnessed in the development of high-efficiency catalysts imparting satisfactory methane selectivity at a commercially viable current density. Herein, a unique category of CO2 RR catalysts is fabricated with the yolk-shell nanocell structure, comprising an Ag core and a Cu2 O shell that resembles the tandem nanoreactor. By fixing the Ag core and tuning the Cu2 O envelope size, the CO flux arriving at the oxide-derived Cu shell can be regulated, which further modulates the *CO coverage and *H adsorption at the Cu surface, consequently steering the CO2 RR pathway. Density functional theory simulations show that lower CO coverage favors methane formation via stabilizing the intermediate *CHO. As a result, the best catalyst in the flow cell shows a high CH4 Faraday efficiency of 74 ± 2% and partial current density of 178 ± 5 mA cm- 2 at -1.2 VRHE , ranking above the state-of-the-art catalysts reported today for methane production. These findings mark the significance of precision synthesis in tailoring the catalyst geometry for achieving desired CO2 RR performance.

9.
Biometrics ; 2021 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-34086345

RESUMO

An adaptive treatment length strategy is a sequential stage-wise treatment strategy where a subject's treatment begins at baseline and one chooses to stop or continue treatment at each stage provided the subject has been continuously treated. The effects of treatment are assumed to be cumulative and, therefore, the effect of treatment length on clinical endpoint, measured at the end of the study, is of primary scientific interest. At the same time, adverse treatment-terminating events may occur during the course of treatment that require treatment be stopped immediately. Because the presence of a treatment-terminating event may be strongly associated with the study outcome, the treatment-terminating event is informative. In observational studies, decisions to stop or continue treatment depend on covariate history that confounds the relationship between treatment length on outcome. We propose a new risk-set weighted estimator of the mean potential outcome under the condition that time-dependent covariates update at a set of common landmarks. We show that our proposed estimator is asymptotically linear given mild assumptions and correctly specified working models. Specifically, we study the theoretical properties of our estimator when the nuisance parameters are modeled using either parametric or semiparametric methods. The finite sample performance and theoretical results of the proposed estimator are evaluated through simulation studies and demonstrated by application to the Enhanced Suppression of the Platelet Receptor IIb/IIIa with Integrilin Therapy (ESPRIT) infusion trial data.

10.
Electrophoresis ; 2021 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-34180069

RESUMO

Microporous organic networks (MONs) that exhibit good stability and hydrophobicity are promising candidates for performing HPLC separation of small organic compounds. However, their applications in separating large analytes as well as biomolecules are still limited by the microporous nature of MONs. Herein, we demonstrated the fabrication of a MON-functionalized silica (MON@SiO2 ), exhibiting micro and mesopores for the HPLC separations of small drugs as well as large analytes, such as flavones, nonsteroidal anti-inflammatory drugs (NSAIDs), endocrine disrupting chemicals (EDCs), and proteins. MON was successfully modified on SiO2 microspheres to yield the uniform and mono-dispersed MON@SiO2 . The separation mechanisms and performance of the MON@SiO2 packed column were evaluated for a wide range of analytes, including neutral, acidic, basic compounds, drugs, and proteins. Compared with commercial C18 and SiO2 -NH2 packed columns, the proposed MON@SiO2 column afforded superior performance in the separations of flavones, NSAIDs, EDCs, and proteins. Moreover, the MON@SiO2 column also offered good repeatability with intraday RSDs (n = 7) of <0.1%, <2.0%, <2.3%, and <0.7% for the retention time, peak height, peak area, and half peak width, respectively, for separating EDCs. This work proved the potential of using MONs in the HPLC separations of drugs and proteins.

11.
IEEE Trans Image Process ; 30: 6485-6497, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34110994

RESUMO

Deep neural networks are fragile under adversarial attacks. In this work, we propose to develop a new defense method based on image restoration to remove adversarial attack noise. Using the gradient information back-propagated over the network to the input image, we identify high-sensitivity keypoints which have significant contributions to the image classification performance. We then partition the image pixels into the two groups: high-sensitivity and low-sensitivity points. For low-sensitivity pixels, we use a total variation (TV) norm-based image smoothing method to remove adversarial attack noise. For those high-sensitivity keypoints, we develop a structure-preserving low-rank image completion method. Based on matrix analysis and optimization, we derive an iterative solution for this optimization problem. Our extensive experimental results on the CIFAR-10, SVHN, and Tiny-ImageNet datasets have demonstrated that our method significantly outperforms other defense methods which are based on image de-noising or restoration, especially under powerful adversarial attacks.

12.
Hepatology ; 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34110633

RESUMO

BACKGROUND & AIMS: Liver metastasis is a frequent occurrences in patients with colorectal cancer (CRC), 15%-25% of CRC patients have liver metastases at the time of initial diagnosis (1). Specifically, some regional-stage patients with mild symptoms (stage Ⅰ or II) will also advance to liver metastases rapidly, even CRC lesion in situ is resected in time. Nevertheless, the precise mechanism of liver metastasis is still unclear. APPROACH & RESULTS: Fresh tumor tissues from CRC patients, adjacent noncancerous tissues (ANT) and colorectal adenoma tissues (ADE) were subjected to microarray analysis to identify differentially expressed miRNA. Exosomes from human serum and cell culture medium were separated, quantitated and verified by transmission electronic microscopy and Zetasizer Nano. Luciferase reporter assay, real-time qPCR, western blot, immunoprecipitation, chromatin and re-chromatin immunoprecipitation, migration and invasion assay, PDX mice model, flow cytometry, immunohistochemistry and immunofluorescence staining were employed to explore the regulation among CRC liver metastases, immunosuppression and cell adhesion. In this study, we demonstrated that the hypoxic microenvironment in primary CRC lesions boosted exosome release, selectively initiated a favorable pre-metastatic niche formation in the hepar, but not in other organs. Mechanistically, Kupffer cells (KCs) can phagocytose exosomes contained highly expressed miR-135a-5p from blood circulation into the liver. The exosomal miR-135a-5p initiated LATS2-YAP-MMP7 axis to promote the occurrences of CRC liver metastasis, and CD30-TRAF2-p65-mediated immunosuppression signaling also contributed to this process. CONCLUSIONS: Hypoxia-induced exosomal miR-135a-5p correlates with the development, clinical severity and prognosis of CRC liver metastases through the pre-metastatic niche, and our findings revealed that miR-135a-5p might be a promising target in halting CRC liver metastases.

13.
Elife ; 102021 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-34085927

RESUMO

Meniscal tears are associated with a high risk of osteoarthritis but currently have no disease-modifying therapies. Using a Gli1 reporter line, we found that Gli1+ cells contribute to the development of meniscus horns from 2 weeks of age. In adult mice, Gli1+ cells resided at the superficial layer of meniscus and expressed known mesenchymal progenitor markers. In culture, meniscal Gli1+ cells possessed high progenitor activities under the control of Hh signal. Meniscus injury at the anterior horn induced a quick expansion of Gli1-lineage cells. Normally, meniscal tissue healed slowly, leading to cartilage degeneration. Ablation of Gli1+ cells further hindered this repair process. Strikingly, intra-articular injection of Gli1+ meniscal cells or an Hh agonist right after injury accelerated the bridging of the interrupted ends and attenuated signs of osteoarthritis. Taken together, our work identified a novel progenitor population in meniscus and proposes a new treatment for repairing injured meniscus and preventing osteoarthritis.

14.
Dalton Trans ; 2021 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-34079971

RESUMO

A family of cyano-bridged 3d-4f 1D chain compounds, {RE[TM(CN)6(2-PNO)5]}·(H2O)4 {RE = YIII, TM = [FeIII]LS (1); RE = DyIII, TM = CoIII (3); RE = ErIII, TM = [FeIII]LS (4), CoIII (5); 2-PNO = 2-picoline-N-oxide} and {RE[TM(CN)6(2-PNO)5]} {RE = DyIII, TM = [FeIII]LS (2)}, were synthesized and characterized. Single-crystal X-ray diffraction studies reveal that compounds 1 and 3-5 are isostructural, while compound 2 has a similar 1D chain structure with a different chain to chain arrangement. An axially-elongated pentagonal bipyramidal (D5h) coordination geometry is formed with five 2-PNO ligands in the equatorial plane and two [TM(CN)6]3- on the apical sites around the rare earth ions in these compounds. A comparison of the magnetic relaxation behaviour in detail reveals that it is more favorable for the Er (4 and 5) than the Dy analogues (2 and 3) to exhibit SIM properties in this axially-elongated D5h coordination environment. Under zero dc field, ac susceptibility measurements show that the Dy analogues have no magnetic relaxation behaviour, while the Er analogues exhibit frequency dependence despite the strong QTM effect. Under a 1 kOe dc field, the Er analogues generally show 1-2 orders of magnitude longer relaxation time at each selected temperature and a higher relaxation energy barrier than the Dy analogues. And the RECo compounds (3 and 5) show a more suppressed QTM effect than the corresponding REFe (2 and 4) compounds, which may be ascribed to the elimination of the fluctuation field from the neighbouring [FeIII]LS ions. The ab initio calculations indicate the misplacement between the orientation of the main magnetic axis and the structural axis in the Dy analogues, and the relative consistency in the Er analogues, which should be the source of the Er analogues showing better SIM properties than the Dy analogues.

15.
Artigo em Inglês | MEDLINE | ID: mdl-34133240

RESUMO

Leukocyte recruitment is a critical step in the pathogenesis of inflammatory and immunological responses. Cell adhesion molecules (CAMs) are involved in controlling cell movements and the recruitment process, and the integrin family of CAMs plays a key role. During cell movement, integrin function is dynamically and precisely regulated. However, this balance might be broken under pathological conditions. Thus, the functional regulation and molecular mechanisms of integrins related to diseases are often a focus of research. Integrin ß2 is one of the most commonly expressed integrins in leukocytes that mediates leukocyte adhesion and migration, and it plays an important role in immune responses and inflammation. In this review, we focus on specific functions of integrin ß2 in leukocyte recruitment, the conformational changes and signal transduction of integrin ß2 activation, and how new insights into these processes can inform future therapies for inflammation and immune diseases.

16.
Nat Prod Res ; : 1-7, 2021 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-34121549

RESUMO

Two new prenylaromadendrane-type diterpenoids, and three known analogues, were isolated from the ethanol extract of the gum resin of B. sacra Flueck. The structures of the new compounds were elucidated using 1 D and 2 D NMR spectroscopic analyses, mass spectrometric data, circular dichroism spectra, and comparison with the other compounds in the literature. One diterpenoid represents the first example of an acetoxyl-substituted prenylaromadendranoid in frankincense. All compounds exhibited notable cytotoxicity against human malignant glioma (U87-MG) cell line, with inhibitory rates exceeding that of the positive control 5-fluorouracil. However, nitric oxide inhibition induced by lipopolysaccarides was not observed in primary mouse peritoneal macrophages.

17.
Artigo em Inglês | MEDLINE | ID: mdl-34109719

RESUMO

Achieving high-current-density and high-area-capacity operation of Li metal anodes offers promising opportunities for high-performing next-generation batteries. However, high-rate Li deposition suffers from undesired Li-ion depletion especially at the electrolyte-anode interface, which compromises achievable capacity and lifetime. Here, electronegative graphene quantum dots are synthesized and assembled into an ultra-thin overlayer capable of efficient Li-ion adsorbing at the nanoscale on Li-metal to fully relieve Li-ion depletion. The protected Li anode achieves long-term reversible Li plating/stripping over 1000 h at both superior current density of 60 mA cm-2 and areal capacity of 60 mAh cm-2 . Implementation of the protected anode allows for the construction of Li-air full battery with both enhanced rate capability and cycling performance.

18.
ACS Appl Mater Interfaces ; 13(25): 29269-29280, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34143595

RESUMO

Nanoantibacterial agents based on catalytic activity were limited due to the low levels of endogenous H2O2 in the microenvironment of bacterial biofilms. However, the additional H2O2 will trigger more side effects to healthy surroundings, which is still a great challenge. Herein, we report an acid-induced self-catalyzing platform based on dextran-coated copper peroxide nanoaggregates (DCPNAs) for antibiofilm and local infection therapy applications. The dextran-functionalized DCPNAs were mediated and conveniently purified via a dextran and ethanol precipitation method, which can also cluster nanodots into nanoaggregates and show good penetrability as well as biocompatibility. Bacterial biofilms were inhibited and destroyed by the reactive oxygen species generated from the Fenton reaction between the Cu2+ and H2O2 released from DCPNAs in an acidic environment, which did not require additional H2O2. As expected, the DCPNAs exhibit low cytotoxicity and excellent acid-induced antibacterial and antibiofilm ability. Moreover, the DCPNAs realized great therapeutic outcomes in the application for in vivo wound healing. The overall excellent properties associated with the DCPNAs highlight that they could be considered as a kind of ideal antimicrobial agents for microbial biofilm infection treatment.

19.
Urol Oncol ; 2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34175215

RESUMO

PURPOSE: This study aimed to investigate if preoperative assessments of multiparametric magnetic resonance imaging (mpMRI) and Magnetic resonance imaging /ultrasound (MRI/US) fusion-guided prostate biopsy could be used to guide focal therapy for prostate cancer. MATERIALS AND METHODS: A total of 101 prostate cancer patients undergoing radical prostatectomy were included. Preoperative findings included mpMRI and MRI/US fusion-guided prostate biopsy, while postoperative whole mount pathology was based on surgical specimen. RESULTS: Of the 101 patients preoperatively diagnosed with a unilateral tumor, postoperative whole mount pathology showed 73.27% were bilateral tumors, and 71.62% of bilateral lesions were clinically significant. Comparison between preoperative and postoperative findings, the correct rate of preoperative mpMRI on the lesion side (left or right) was only 20.79%. As for the Gleason score, the correct rate of preoperative MRI/US fusion-guided prostate pathology was 67.33%. Judging from postoperative whole mount pathology, 47.52% of patients had a unilateral clinically significant tumor, which is an indication for focal therapy. CONCLUSION: Preoperative examinations of mpMRI and MRI/US fusion-guided prostate biopsy cannot be used to guide focal therapy for prostate cancer.

20.
Spectrochim Acta A Mol Biomol Spectrosc ; 261: 120037, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34116417

RESUMO

A new 1,8-naphthalimide-based Schiff base compound, named as (Z)-2-butyl-6-(((2-hydroxyphenyl)imino)methyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione (BHBD), has been simply synthesized with high yields. BHBD can be employed as a "turn-on" fluorescent probe for Cu2+ ion with high sensitivity, high selectivity and relatively low detection limit (0.48 × 10-6 M). The fluorescence emission of BHBD is very weak in H2O/THF (v/v: 7/3) mixture, which is significantly enhanced after addition of Cu2+ ion. The proposed mechanism is verified by 1H NMR, Job's plot and TOF-MS experiments. Anti-interference experiment, cytotoxicity assay and pH influence results indicated that BHBD meets the requirements of bioimaging. Therefore, BHBD has been successfully applied in detecting Cu2+ ion in HeLa cells.


Assuntos
Corantes Fluorescentes , Naftalimidas , Células HeLa , Humanos , Espectrometria de Fluorescência
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