Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.535
Filtrar
1.
Virulence ; 10(1): 910-924, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31680614

RESUMO

Thiol-disulfide glutaredoxin systems of bacterial cytoplasm favor reducing conditions for the correct disulfide bonding of functional proteins, and therefore were employed by bacteria to defend against oxidative stress. Listeria monocytogenes has been shown to encode a putative glutaredoxin, Grx (encoded by lmo2344), while the underlying roles remain unknown. Here we suggest an unexpected role of L. monocytogenes Grx in oxidative tolerance and intracellular infection. The recombinant Grx was able to efficiently catalyze the thiol-disulfide oxidoreduction of insulin in the presence of DTT as an election donor. Unexpectedly, the deletion of grx resulted in a remarkably increased tolerance and survival ability of this bacteria when exposed to various oxidizing agents, including diamide, and copper and cadmium ions. Furthermore, loss of grx significantly promoted bacterial invasion and proliferation in human epithelial Caco-2 cells and murine macrophages, as well as a notably increasing invasion but not cell-to-cell spread in the murine fibroblasts L929 cells. More importantly, L. monocytogenes lacking the glutaredoxin exhibited more efficient proliferation and recovery in the spleens and livers of the infected mice, and hence became more virulent by upregulating the virulence factors, InlA and InlB. In summary, we here for the first time demonstrated that L. monocytogenes glutaredoxin plays a counterintuitive role in bacterial oxidative resistance and intracellular infection, which is the first report to provide valuable evidence for the role of glutaredoxins in bacterial infection, and more importantly suggests a favorable model to illustrate the functional diversity of bacterial Grx systems during environmental adaption and host infection.

2.
Medicine (Baltimore) ; 98(44): e17867, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689880

RESUMO

Glycogen storage disease (GSD) type IX, characterized by liver enlargement and elevated aminotransferase levels, is the most frequent type of GSD. The global incidence of GSD type IXa is only about 1/100,000 individuals. Case reports of GSD type IX are rare in China. We present the first case report of GSD type IXa in Northeast China caused by mutation of PHKA2.An 11-year-old boy was referred to our hospital because of liver enlargement with consistently elevated transaminase levels over 6 months.Histopathological results following an ultrasound-guided liver biopsy confirmed a diagnosis of GSD. Further genetic testing showed that the patient had GSD type IXa caused by the c.133C>T mutation in PHAK2.We placed the patient on a high-protein and high-starch diet and provided hepatoprotective and supportive therapy.The patient's transaminase levels decreased significantly and were nearly normal at 10-month follow-up.This is the first reported case of GSD type IXa in Northeast China. We hope that the detailed and complete report of this case will provide a reference for the diagnosis of liver enlargement of unknown etiology in future clinical practice.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31704215

RESUMO

BACKGROUND: Combining aminoglycosides with ß-lactam antibiotics for treating serious infections has not been associated with reduced mortality in previous meta-analyses. However, the multiple-daily aminoglycoside dosing regimen principally used in most included studies is inconsistent with current practice. OBJECTIVE: To determine if a combination of an aminoglycoside administered as a single-daily dose and a ß-lactam antibiotic reduces all-cause mortality in patients compared with ß-lactam antibiotic monotherapy. METHODS: A systematic review and meta-analysis of clinical studies was performed (Prospero registration number #68506). Studies were included if they compared ß-lactam antibiotic monotherapy with combined ß-lactam and single daily dose aminoglycoside therapy for treating serious infections. Studies investigating multiple daily dosing aminoglycoside regimens were excluded as were studies investigating infective endocarditis or febrile neutropaenia. Study quality was assessed using the PEDro and Newcastle-Ottawa scoring systems. The end points for outcome analyses were 30-day all-cause mortality, clinical cure and nephrotoxicity. RESULTS: Four randomised controlled trials and five retrospective cohort studies were analysed. Compared to ß-lactam antibiotic monotherapy, single-daily aminoglycoside dosing in combination with ß-lactam antibiotics was not associated with reduced mortality compared with ß-lactam antibiotic monotherapy (n=3686, OR 0.82, 95% CI 0.63-1.08, p=0.10, I2 42%). A subgroup analysis of cohort studies suggested a reduced mortality with combination therapy (n=3563, OR 0.79, 95% CI 0.64-0.99, p=0.04, I2 32%). No increased risk of nephrotoxicity was identified (n=1110, OR 1.31, 95% CI 0.83-2.09, p=0.40, I2 0%). CONCLUSIONS: Existing evidence suggests no added survival benefit from a single daily dosing regimen of an aminoglycoside when combined with ß-lactam antibiotics.

4.
EBioMedicine ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31668569

RESUMO

BACKGROUND: Bronchiolitis obliterans syndrome (BOS) after allo-HSCT is a devastating complication with limited therapeutic options. We aimed to assess the efficacy and safety of mesenchymal stem cells (MSCs) in BOS after allo-HSCT. METHODS: This multicenter prospective cohort study enrolled 81 allo-HSCT recipients whose BOS were diagnosed within 6 months. The choice of prednisone and azithromycin combined with or without MSCs was based on patient preferences (MSC n = 49, non-MSC n = 32). The primary endpoint was response rate at 3 months, defined as the proportion of patients achieving FEV1 improvement or steroid sparing. The trial was registered at ClinicalTrials.gov (NCT02543073). FINDINGS: Response rate was 35/49 patients (71%, 95% CI 59 to 84%) and 14/32 (44%, 27 to 61%) in MSC and non-MSC group, respectively (p = 0.013). The addition of MSCs was associated with a better difference for change in FEV1 rate of decline, compared to non-MSC group (53 mL/months, 2 to 103; p = 0.040). The 3-year overall survival post-diagnosis was 70.6% (55.9 to 85.3%) and 58.2% (36.1 to 78.5%) in MSC and non-MSC group, respectively (p = 0.21). Clinical improvement was accompanied by a significant increase of interleukin (IL)-10-producing CD5+B cells. There was no statistical difference in the rates of infections and leukemia relapse between the two groups. MSCs were well-tolerated with no serious adverse events. INTERPRETATION: MSCs offer an effective and safe therapeutic option for BOS after allo-HSCT. Our study strengthens evidence for clinical use of MSC therapy in BOS. These data also provide novel insight into potential biological mechanisms of MSC treatment and support further investigation in larger randomized controlled trials. FUNDING: National Key R&D Program of China, National Natural Science Foundation of China, Health Collaborative Innovation Major Projects of Guangzhou City, Science and Technology Planning Project of Guangdong Province.

5.
Heredity (Edinb) ; 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676879

RESUMO

The center-periphery hypothesis (CPH) states that the genetic diversity, genetic flow, and population abundance of a species are highest at the center of the species' geographic distribution. However, most CPH studies have focused on the geographic distance and have ignored ecological and historical effects. Studies using niche models to define the center and periphery of a distribution and the interactions among geographical, ecological, and historical gradients have rarely been done in the framework of the CPH, especially in biogeographical studies of animal species. Here, we examined the CPH for a widely distributed arthropod, Tetranychus truncatus (Acari: Tetranychidae), in eastern China using three measurements: geographic distance to the center of the distribution (geography), ecological suitability based on current climate data (ecology), and historical climate data from the last glacial maximum (history). We found that the relative abundances of different populations were more strongly related to ecology than to geography and history. Genetic diversity within populations and genetic differentiation among populations based on mitochondrial marker were only significantly related to history. However, the genetic diversity and population differentiation based on microsatellites were significantly related to all three CPH measurements. Overall, population abundance and genetic pattern cannot be explained very well by geography alone. Our results show that ecological gradients explain the variation in population abundance better than geographic gradients and historical factors, and that current and historical factors strongly influence the spatial patterns of genetic variation. This study highlights the importance of examining more than just geography when assessing the CPH.

6.
J Glob Health ; 9(2): 020702, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31673344

RESUMO

Methods: Using an interrupted time series design, we conducted segmented regression analyses of utilization changes of targeted anti-cancer medicines covered by the provincial government health insurance program during 2013 to 2016 in 69 hospitals with more than 100 beds in Hangzhou, the capital city of Zhejiang province of China. The WHO/Health Action International Project on Medicine Prices and Availability methodology was used to measure patient affordability of the study medicines. Results: In March 2015, the utilization of all study medicines increased by 15.58 (95% CI = 3.86, 27.30, P = 0.01) to 439.14 standard units (95% CI = 311.79, 566.49, P < 0.001). Before covered by government health insurance, the estimated out-of-pocket payment by patient ranged from 3.0 to 13.1 times of the provincial average disposable annual income per capita for urban residents, and 6.2 to 27.3 times for rural residents. Such payments were reduced to 0.6 to 2.1 times for urban residents and 1.8 to 4.4 times for rural population after government health insurance coverage inclusion. During 2015 to 2016, the per capita contribution to Hangzhou catastrophic health insurance program was CNY15 (US$ 2.3), and the reimbursement rate was 70% in Hangzhou city. The cumulative total insurance expenses on six study targeted anticancer medicines accounted for an estimated 53% of the total amount of premiums of the government catastrophic health insurance fund. Sensitivity analyses indicated that this proportion would have changed to 46%, 61% and 69% when changing the per capita contribution to CNY25 (US$ 3.8) and CNY40 (US$ 6.2), and changing the insurance reimbursement rate to 60%, 80 and 90%. Conclusion: Government health insurance coverage inclusion significantly increased utilization of the expensive targeted anti-cancer medicines, and improved patient affordability. However, the financial burden of patients is still high, especially for the rural low-income population. Rising utilization and expenditures call for careful monitoring of anti-cancer medicines use, and for strategies to decrease prices to facilitate medicines access and keep the insurance system sustainable.

7.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117675, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31670047

RESUMO

By modifying the 10-butyl-2-methoxy-10H-phenothiazine-3-carbaldehyde with malonontrile group, a new fluorescent sensor PBM for selective detection of hydrazine in ratiometric mode has been developed. Probe PBM owned the advantages of quick response (10 min), remarkable Stokes shift (168 nm for PBM, 161 nm for PBM-NH2), excellent selectivity, high sensitivity (detection limit of 63.2 nM was obtained from in vitro experiment), profound ratiometric change (82-fold) and low cytotoxicity in response to hydrazine. Additionally, it could be utilized to monitor hydrazine in gas state with various concentrations through vivid color changes and imaged hydrazine in living MCF-7 cells with excellent performance.

8.
Water Res ; 169: 115208, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31670088

RESUMO

For the control of sulfide and methane in sewers, it is favorable to reduce their production rather than to remove them after generation. In this study, we revealed rapid and strong biocidal effect of ferrate (Fe(VI)) on sulfidogenic and methanogenic sewer biofilms, leading to control of sulfide and methane production in sewer. The inactivation of the microorganisms in sewer biofilms by Fe(VI) could be accomplished within 15 min for a single dosing event and the biocidal effect could be enhanced by applying pulse dosing strategy. The microbiological analysis showed that the key functional genes involved in sulfide and methane production, i.e. dsrA and mcrA, in the viable cells after Fe(VI) dosing were decreased substantially by 84.2% and 86.6%, respectively. Significant drops were also observed in the relative abundances of viable sulfide reducing bacteria (SRB) and methanogenic archaea (MA). The direct dosing of Fe(VI) into a sewer reactor led to instant and complete suppression of sulfidogenic and methanogenic activities, and the recovery of the activities resembled the regrowth of residual SRB and MA. The results of this study suggested the feasibility for developing an efficient and cost-effective sulfide and methane control strategy using Fe(VI).

9.
Chemosphere ; 242: 125172, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31675584

RESUMO

Thallium (Tl) is an extremely toxic metal with high risk. Sediment samples from a well dated sediment core retrieved from a heavily polluted urban lake were measured to assess the magnitude of Tl pollution and reveal its transfer mechanisms within the lake. The results show that the lake has experienced serious Tl pollution during the past 60 years, owing to a large influx of Tl-bearing wastes mostly from a neighboring copper (Cu) smelter. The Tl contents in the sediment core at different depths generally correlated positively with the production scale of Cu smelting activities in the past. Further mineralogical analysis on the electrostatic precipitator dust from the Cu smelter and highly-polluted samples from the sediment core by electronic microscopy and X-ray diffraction suggested that Tl was primarily bound to quartz and aluminosilicates. Large quantities of Tl were also observed in diatom frustules collected from the sediments, suggesting a potentially significant role of biogenic silicates in the uptake and transfer of Tl in the sediment-water system. The findings indicate a new and emerging pollution source of thallium arising from copper metallurgy activities. The results also highlight the necessity of strengthened monitoring and regulations towards Tl pollution in environmental systems impacted by Cu smelting activities in China and other nations.

10.
Water Res ; 169: 115218, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31677435

RESUMO

Effective sludge treatment with bioenergy production is attracting increasing interests as large quantities of waste activated sludge (WAS) are produced during the wastewater treatment. In this study, a new biotechnical process for converting the WAS alkaline fermentation liquor (WASAFL) into valuable, easy-separated medium chain fatty acids (MCFAs) through chain elongation (CE) was investigated, which may provide a new insight into sludge treatment. In the process, ethanol was served as the electron donor (EDs) and WASAFL were main electron acceptors (EAs). The MCFAs productions were investigated under three different ED to EA ratios (i.e., 1:2, 1:1 and 2:1). The result showed that MCFAs production was increased from 2.88 ±â€¯0.01 to 5.28 ±â€¯0.18 g COD/L with the increase of ED to EA ratio. However, the highest MCFA selectivity was achieved at 72.9% when the ED to EA ratio was 1:1. The decrease in the selectivity at high ED:EA ratio is mainly due to the production of higher alcohol (i.e., n-butanol and n-hexanol). The thermodynamic analysis confirmed all CE processes for MCFAs production from WASAFL were exothermic reactions, with the spontaneity and energy release of the reactions increased with the ethanol level. The microbial community analysis showed that the relative abundances of Clostridium, Oscillibacter, Leptolinea and Exilispira were positively correlated with the MCFAs production. The metagenomic analysis suggested that both the reverse ß-oxidization pathway and fatty acid biosynthesis pathway contributed to the CE process in the studied system. The functional enzymes were mainly associated within Clostridium, with Clostridium Kluyveri, Clostridium botulinum and Clostridium magnum being likely the key species responsible for the CE process.

11.
Atherosclerosis ; 291: 27-33, 2019 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-31683090

RESUMO

BACKGROUND AND AIMS: Lipoprotein(a) [Lp(a)] has been considered as a causal risk factor for cardiovascular disease (CVD) in the general population and levels vary in different ethnicities. However, no systemic analysis is currently available regarding the relation of plasma Lp(a) levels to cardiovascular events (CVEs) in Chinese patients with heterozygous familial hypercholesterolemia (HeFH). METHODS: Three hundred and ninety-three patients with HeFH undergoing Lp(a) measurement at baseline were consecutively enrolled and followed prospectively for an average of 36.5 months. Lp(a) levels were determined using an immunoturbidimetry assay. Cox regression analysis with adjusted hazard ratios (HRs) and Kaplan-Meier analysis were used to evaluate the prognostic value of Lp(a) on CVEs. RESULTS: Thirty-five events occurred during follow-up. Lp(a) was significantly higher in patients with CVEs (53.3 mg/dL versus 31.7 mg/dL, p < 0.001). In Kaplan-Meier analysis, patients with upper tertile of Lp(a) had a significant lower event-free survival (p = 0.004). After adjusting for confounding risk factors, per log unit increase in baseline Lp(a) was independently associated with CVEs [HR: 2.03(1.28-3.21), p = 0.002]. HRs remained unchanged after accounting for hard endpoints and did not vary too much in several relevant subgroups. Adding Lp(a) to the Cox model led to a significant improvement in C-statistic, net reclassification and integrated discrimination. Moreover, HR for upper versus lower tertile of change in Lp(a) was 2.68 (1.11-6.48) for CVEs after one year. CONCLUSIONS: Both baseline and on-statin treatment Lp(a) levels were associated with an increased risk of CVEs in patients with HeFH, suggesting that Lp(a) measurement might clinically help further risk stratification of FH patients.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31699816

RESUMO

Three-dimensional hierarchical morphologies widely exist in natural and biomimetic materials, which impart preferential functions including liquid and mass transport, energy conversion, and signal transmission for various applications. While notable progress has been made in the design and manufacturing of various hierarchical materials, the state-of-the-art approaches suffer from limited materials selection, high costs, as well as low processing throughput. Herein, by harnessing the configurable elastic crack engineering-controlled formation and configuration of cracks in elastic materials-an effect normally avoided in various industrial processes, we report the development of a facile and powerful technique that enables the faithful transfer of arbitrary hierarchical structures with broad material compatibility and structural and functional integrity. Our work paves the way for the cost-effective, large-scale production of a variety of flexible, inexpensive, and transparent 3D hierarchical and biomimetic materials.

13.
Carbohydr Polym ; 226: 115283, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31582088

RESUMO

Deproteinization is a crucial step for the purification of polysaccharides from natural biomass. However, traditional deproteinization technologies often suffer from complicated operating processes and the usage of toxic chemical reagents. Herein, an advanced and universal deproteinization method based on dual-functional adsorbent consisted of preferential protein adsorption coating of tannic acid-FeIII (TA-FeIII) and magnetic Fe3O4 (Fe3O4@TA-FeIII, abbreviated as FTF NPs) was developed. The proposed FTF NPs showed remarkable efficiency to remove protein from Lycium barbarum L. polysaccharides (LBPs) with deproteinization ratio of 96%, higher than the typical Sevag method (85%). Detailed adsorption kinetics studies demonstrated deproteinization process reached equilibrium after 10 min, faster than other reported deproteinization adsorbents. Furthermore, FTF NPs are structurally and functionally nondestructive as regards LBPs without using organic reagents. Also, it exhibited widespread-use deproteinization performance to several common plant species. Therefore, the proposed nano-separation based on TA-FeIII complex is an advanced and universal tool to high-efficiently deproteinize plant polysaccharides.

14.
J Psycholinguist Res ; 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31583601

RESUMO

Chinese coordinative compound words are common and unique in inter-character semantic and orthographic relationships. This study explored the inter-character orthographic similarity effects on the recognition of transparent two-morpheme coordinative compound words. Seventy-two native Chinese readers participated in a lexical decision task. The findings demonstrated robust inhibitory inter-character orthographic similarity effects, intra-word character reversal effects, and inter-character semantic similarity effects. These results were compared to those of previous studies on coordinative compound word recognition and on the orthographic similarity phenomenon at both character and word levels. The findings were explained with the multi-level representational model of morphological processing of Chinese compound words (Zhou and Marslen-Wilson in Psychologia 43:47-66, 2000). The model was further extended by adding the activation of morpho-orthographic relationships and the mapping of morphemic orthographic information onto the semantic information of both morphemes and whole words.

15.
J Dig Dis ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31574578

RESUMO

OBJECTIVE: To evaluate the current status of the diagnosis and treatment of gastroesophageal reflux disease (GERD) in patients with nocturnal gastroesophageal reflux (nGER). METHODS: This multicenter and observational study was conducted in China from May 2017 to February 2018. We selected outpatients with nGER and collected relevant data by using a questionnaire. The general information including age, sex, height, weight, history of smoking and drinking, co-morbid diseases, lifestyle, self-reported health status, medical history, nGER symptoms and severity, Hospital Anxiety and Depression Scale (HADS), Pittsburgh Sleep Quality Index (PSQI), diagnosis, and treatment choice were collected. The registrated number in Chinese Clinical Trial Registry is ChiCTR1800017525. RESULTS: The study included 4978 individuals, of them, 4374 (87.9%) completed the questionnaire. The symptoms of heartburn and regurgitation were more severe during nighttime than daytime (P < 0.05). Age and body mass index were positively correlated with reflux severity in daytime and nighttime (P < 0.05). There was a positive correlation between nGER severity and lifestyle factors such as smoking, high-fat diet, carbonated beverages, late supper (later than 9 pm), and snoring during sleeping (P < 0.05). Nighttime heartburn and regurgitation were positively correlated with sleep disorder. CONCLUSION: Some lifestyle factors were associated with nGER severity, and nGER affected sleep quality. It will be beneficial to popularize and strengthen the diagnosis and treatment of nGER.

16.
Eur J Prev Cardiol ; : 2047487319880985, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31604401

RESUMO

AIMS: Familial hypercholesterolemia patients are characterized by early onset of coronary artery calcification and atherosclerosis, and high incidence of cardiovascular events. Plasma proprotein convertase subtilisin/kexin type 9 was reported to be a predictor for cardiovascular risk in the general population. However, its prognostic value for predicting recurrent cardiovascular events in familial hypercholesterolemia patients remains undetermined. METHODS: A total of 249 patients with molecularly and/or clinically (Dutch Lipid Clinic Network score > 6) defined familial hypercholesterolemia who had experienced a first cardiovascular event were consecutively included and plasma proprotein convertase subtilisin/kexin type 9 concentrations were measured by enzyme-linked immunosorbent assay. Coronary artery calcification was measured using Agatston method and coronary severity was assessed by Gensini score, respectively. All patients received standard lipid-lowering therapy and were followed-up for recurrent cardiovascular events. Univariate and multivariate regression and Cox analyses was used to calculate hazard ratios with 95% confidence interval. RESULTS: Circulating proprotein convertase subtilisin/kexin type 9 concentrations were positively associated with coronary artery calcification scores and Gensini score by both univariate and multivariate analyses. During a mean follow-up of 43 ± 19 months, 29 (11.51%) recurrent cardiovascular events occurred. Kaplan-Meier analysis showed that patients with the highest proprotein convertase subtilisin/kexin type 9 levels had the lowest event-free survival time. Multivariable Cox regression analysis revealed that proprotein convertase subtilisin/kexin type 9 was independently associated with recurrent cardiovascular events (hazard ratio: 1.45, 95% confidence interval: 1.11-1.88). The combination of proprotein convertase subtilisin/kexin type 9 to Cox prediction model led to an enhanced predictive value for recurrent cardiovascular events. CONCLUSIONS: Increased level of proprotein convertase subtilisin/kexin type 9 was a significant risk factor of atherosclerosis and independently predicted future recurrent cardiovascular events in familial hypercholesterolemia patients receiving standard lipid-lowering treatment.

17.
Med Sci Monit Basic Res ; 25: 218-228, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31645540

RESUMO

BACKGROUND Most eukaryocytes release nano vesicles (30-120 nm), named exosomes, to various biological fluids such as blood, lymph, and milk. Hepatocellular carcinoma (HCC) is one of the tumors with the highest incidence rate in primary malignant carcinoma of the liver. However, the mechanism of HCC proliferation remains elusive. In this study, we aim to explore whether HCC cell-derived exosomes affect the proliferation of cancer cells. MATERIAL AND METHODS Exosomes were isolated from HCC cells by ultracentrifugation and were visualized the phenotype by transmission electron microscopy. Cell proliferation was detected by Cell Counting Kit-8 assays and EdU (5-ethynyl-2-deoxyuridine) incorporation assays. Dual-luciferase assays were performed to validate the paired correlation of miR-155 and 3'-UTR of PTEN (gene of phosphate and tension homology deleted on chromosome 10). A xenograft mice model was constructed to verify the effect of exosome-mediated miR-155 on cell proliferation in vivo. RESULTS Our finding showed that miR-155 was enriched in exosomes released from HCC cells. The exosome-containing miR-155 transferred into new HCC targeted cells and lead to the elevation of HCC cells' proliferation. Besides, the exosomal miR-155 directly bound to 3'-UTR of PTEN leading to the reduction of relevant targets in recipient liver cells. The knockdown of PTEN attenuated the proliferation of HCC cells treated with the exosomal miR-155. Moreover, nude-mouse experiment results revealed a promotional effect of the exosomal miR-155 on HCC cell-acquired xenografts. CONCLUSIONS Our study indicated that exosomal-specific miR-155 transfers to adjacent and/or more distant cells and stimulates the proliferation of HCC cells.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31645666

RESUMO

Mutations of internal tandem duplication in FMS-like tyrosine kinase 3 (FLT3-ITD) contribute to poor prognosis in cytogenetically normal acute myeloid leukemia (CN-AML). Chemotherapy has limited effect, while allogeneic hematopoietic stem cell transplantation (allo-HSCT) plus sorafenib maintenance is a promising protocol to improve their therapeutic outcome. However, the prognostic significance of FLT3-ITD mutant status remains controversial. To investigate this, we detected FLT3-ITD mutant ratio (high and low) and length (long and short) in enrolled 184 CN-AML patients without NPM1 mutation, and evaluated their impact on complete remission (CR), overall survival (OS), relapse-free survival (RFS) and relapse risk (RR) after chemotherapy or allo-HSCT plus sorafenib maintenance. Our studies showed that FLT3-ITD mutation had negative impact on chemotherapeutic response, OS and RFS in CN-AML patients. There was no significant difference in CR rate between high and low ratio, or long and short length. Increasing ITD mutant ratio and length were associated with decreasing OS, and long length had shorter RFS and higher RR than the short after chemotherapy. Allo-HSCT plus sorafenib maintenance was an effective strategy to improve RFS and decrease relapse probability in FLT3-ITD AML patients, and benefited to these regardless of mutant ratio, and those with long length instead of the short.

19.
J Investig Clin Dent ; : e12478, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31638347

RESUMO

AIM: The aim of the present systematic literature review was to evaluate bleeding on probing (BOP), pocket depth (PD), and marginal bone loss (MBL) in type 2 diabetes mellitus (T2DM) control patients. METHODS: An electronic literature search was conducted through PubMed, Web of Science, Cochrane, Embase, and ScienceDirect. The search included prospective human clinical studies that analyzed the success of dental implants in T2DM control patients. For inclusion, studies should have had a minimum of 1-year follow up and should have assessed the following parameters: MBL, BOP and PD. Prior to meta-analysis, all of the studies were assessed for quality, bias, and heterogeneity. RESULTS: Risk of bias analysis indicated that all studies were of moderate quality. After a full-text evaluation, only seven studies met the inclusion criteria for this meta-analysis, with a combined total of 443 patients and 530 dental implants. The meta-analysis indicated a statistically significant difference between parameters of implants placed in the glycemic-controlled group and healthy group in MBL (P < .001), BOP (P < .04), and PD (P < .001). CONCLUSION: The results of the present study indicated that, despite being glycemic controlled, patients with T2DM were associated with a higher risk of peri-implant disease.

20.
Org Biomol Chem ; 17(40): 9008-9013, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31576889

RESUMO

An efficient synthetic protocol for novel indeno[1,2-a]fluorene derivatives was successfully developed by the base promoted domino reaction of 1,3-indanedione with 3-arylideneindolin-2-ones. The domino reaction in different solvents selectively gave carbamato- or amino-substituted indeno[1,2-a]fluorene in satisfactory yields. Additionally, a similar domino reaction of 1,3-indanediones with chalcones also gave polysubstituted indeno[1,2-a]fluorenes in high yields. The reaction mechanism is believed to proceed with base-promoted dimerization of 1,3-indanedione, Michael condensation, annulation, ring-opening of indolin-2-one and aromatization process.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA