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1.
Pathol Res Pract ; : 152593, 2019 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-31471104

RESUMO

The aim of this study was to investigate whether PRRX2 may regulate the liver metastasis of colon cancer via the Wnt/ß-catenin signaling pathway. PRRX2 and ß-catenin in patients with the liver metastases of colon cancer was detected by immunochemistry. Colon cancer cells (CT-26 and CMT93) were divided into Normal, si-Ctrl, si-PRRX2 and si-PRRX2 +LiCl groups. Cell invasive and migrating abilities and the related proteins were detected. Liver-metastatic mice model was constructed consisting of Normal, NC shRNA and PRRX2 shRNA groups to examine the function of PRRX2 shRNA on liver metastasis. We found that PRRX2 and ß-catenin positive rate was elevated in colon cancer tissues, especially in those tissues with liver metastasis, and there was a close relation between PRRX2 and the clinical staging, lymph node metastasis and numbers of liver metastases of colon cancer patients with liver metastasis. In vitro, the invasive and migrating abilities of CT-26 and CMT93 cells decreased apparently in the si-PRRX2 group, with down-regulation of PRRX2, p-GSK3ßSer9/GSK3ß, nucleus and cytoplasm ß-catenin, TCF4 and Vimentin but up-regulation of E-cadherin. However, LiCl, the Wnt/ß-catenin pathway activator, can reverse the inhibitory effect of si-PRRX2 on invasive and migrating ability of colon cancer cells. In vivo, the volume and weight of transplanted tumor and the number of liver metastases in the PRRX2 shRNA group were significantly reduced, with the similar protein expression patterns as in vitro. In a word, PRRX2 inhibition may reduce invasive and migrating abilities to hinder epithelial-mesenchymal transition (EMT), and suppress colon cancer liver metastasis through inactivation of Wnt/ß-catenin pathway.

2.
Ann Thorac Surg ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31494136

RESUMO

BACKGROUND: To evaluate the long-term impact of frozen elephant trunk (FET) on the distal aorta of patients with Marfan syndrome (MFS) sustaining type I dissection confined to the thoracic aorta (above diaphragmatic hiatus). METHODS: Between 2003 and 2016, 42 MFS (Ghent/revised Ghent criteria) patients (age 33.3±8.9 years; 27 men, 64.3%) with type I dissection above diaphragmatic hiatus involving the arch (22 acute, 52.4%) underwent total arch replacement and FET. Dissection extended distally to mid-descending aorta in 32 (76%) and to above diaphragmatic hiatus in 10 (24%). Operative mortality was 4.8% (2/42). Follow-up was 100% at 6.3±3.0 years. RESULTS: Maximal aortic sizes (DMaxs) at mid-descending aorta, diaphragmatic hiatus, renal arteries and largest segment of abdominal aorta were 22.8, 21.1, 19.1 and 19.9 mm preoperatively and 23.1, 22.0, 19.8 and 22.4 mm on latest CTA. Dilation and complete remodeling of distal aorta occurred in 10.0% (4/40) and 90% (36/40), respectively. There were 1 late death and 3 distal reoperations. Preoperative abdominal aortic DMax was predictive of distal dilatation (mm) (hazard ratio, HR=1.78, p=0.021) and reoperation (≥ versus <25 mm) (HR=12.88, p=0.037). At 10 years, freedom from dilation, reoperation and death were 69.8%, 78.1% and 90.0%, respectively. At 8 years, the rates of death, reoperation and reoperation-free survival were 10%, 11% and 79%, respectively. CONCLUSIONS: The FET technique has a positive remodeling impact on type I dissection confined to thoracic aorta in MFS patients. This study adds evidence supporting the safety and durability of this extended approach for aortic dissection in Marfan syndrome.

3.
AAPS PharmSciTech ; 20(7): 302, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31489504

RESUMO

Docetaxel (DTX) was effective in the treatment of neoplasm but could only be administered intravenously with the poor oral bioavailability owing to its undesirable solubility, remarkably metabolic conversion, and other factors. Cimetidine (CMD), a classic CYP3A4 isozyme inhibitor, had exhibited a wide range of inhibition on the metabolism of many drugs. The aim of this study was to construct the novel docetaxel-cimetidine (DTX-CMD) complex and the chitosan-deoxycholate nanoparticles based on it to confirm whether this formulation could show advantages in terms of solubility, dissolution rate, small intestinal absorption, and oral bioavailability in comparison with the pure drug. The solid-state characterization was carried out by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FT-IR), and simultaneous DSC-TGA (SDT). Dissolution rate and kinetic solubility study were determined by evaluating the amount of DTX in distilled water and phosphate buffer solution (pH = 7.4), respectively. And small intestinal absorption and pharmacokinetics study were conducted in rats. The results of this study demonstrated that we successfully constructed DTX-CMD complex and its chitosan-deoxycholate nanoparticles. Furthermore, the DTX-CMD complex increased the solubility of DTX by 2.3-fold and 2.1-fold in distilled water and phosphate buffer solution, respectively. The ultimate accumulative amount of DTX-CMD complex nanoparticles through rat small intestinal in 2 h was approximately 4.9-fold and the oral bioavailability of the novel nanoparticles was enhanced 2.8-fold, compared with the pure DTX. The superior properties of the complex nanoparticles could both improve oral bioavailability and provide much more feasibility for other formulations of DTX.

4.
Chin J Traumatol ; 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31506232

RESUMO

PURPOSE: Through the study of economic, traffic and population data related to road traffic accidents from 2004 to 2016, this paper analyzed the impact of various factors on road traffic casualties in China, and provides theoretical basis and suggestions for the road traffic safety management in China. METHODS: Based on three aspects (economy, road, population) with five factors: gross domestic product (GDP), traffic investment, new vehicle ownership, new road mileage and newly increased population, this paper collected the relevant data of road traffic accidents in 31 provinces and cities in China, from 2004 to 2016. A panel model was established to carry on empirical analysis. RESULTS: All factors have a significant impact on the number of road traffic accident casualties. When other factors remain unchanged, the number of road traffic casualties decreased by an average of 0.19 for every 100 million yuan increased in GDP. For every 100 million yuan increased in traffic investment, the number of road traffic casualties is reduced by an average of 13.93, indicating that economic development can improve road traffic safety to a certain extent. On the contrary, the growth in road mileage, new motor vehicles and population have increased the number of road traffic casualties. For every 10, 000 km of new road mileage, the number of traffic accident casualties has increased by 284.04. For every 10,000 newborns, the number of road traffic casualties increased by 7.33; as the number of new motor vehicles increases by 10,000, the number of road traffic casualties increased by an average of 21.77. CONCLUSION: The increase of GDP and traffic investment can significantly reduce the number of road traffic casualties in China, which shows that economic development is essential to improve road traffic safety. The number of new road mileage, newly increased population and the new motor vehicles are positively correlated with the number of traffic accident casualties in traffic accidents, which reflects the existing problems in road design, distribution of road resources, and traffic management in China. Therefore, it is necessary to improve the economic and road related aspects to improve road traffic safety.

5.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(9): 897-900, 2019 Sep 10.
Artigo em Chinês | MEDLINE | ID: mdl-31515785

RESUMO

OBJECTIVE: To explore the genetic basis for a case of recurrent fetal congenital hydrocephalus. METHODS: Next-generation sequencing was carried out for the fetus, the gravida and two of her sisters. RESULTS: The fetus was found to harbor a c.1765T>C (p.Tyr589His) mutation in exon 14 of the L1CAM gene, which was derived from the gravida. CONCLUSION: Male fetuses with recurrent hydrocephalus should be subjected to testing of the L1CAM gene to facilitate genetic counseling and prenatal diagnosis.

6.
Mol Cancer ; 18(1): 135, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31492160

RESUMO

BACKGROUND: Emerging studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in colorectal cancer (CRC). Here, we report a lncRNA, SATB2-AS1, which is specifically expressed in colorectal tissue and is significantly reduced in CRC. We systematically elucidated its functions and possible molecular mechanisms in CRC. METHODS: LncRNA expression in CRC was analyzed by RNA-sequencing and RNA microarrays. The expression level of SATB2-AS1 in tissues was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). The functional role of SATB2-AS1 in CRC was investigated by a series of in vivo and in vitro assays. RNA pull-down, RNA immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP), chromatin isolation by RNA purification (ChIRP), Bisulfite Sequencing PCR (BSP) and bioinformatics analysis were utilized to explore the potential mechanisms of SATB2-AS1. RESULTS: SATB2-AS1 is specifically expressed in colorectal tissues and downregulated in CRC. Survival analysis indicates that decreased SATB2-AS1 expression is associated with poor survival. Functional experiments and bioinformatics analysis revealed that SATB2-AS1 inhibits CRC cell metastasis and regulates TH1-type chemokines expression and immune cell density in CRC. Mechanistically, SATB2-AS1 directly binds to WDR5 and GADD45A, cis-activating SATB2 (Special AT-rich binding protein 2) transcription via mediating histone H3 lysine 4 tri-methylation (H3K4me3) deposition and DNA demethylation of the promoter region of SATB2. CONCLUSIONS: This study reveals the functions of SATB2-AS1 in CRC tumorigenesis and progression, suggesting new biomarkers and therapeutic targets in CRC.

7.
Bioresour Technol ; 293: 122009, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31493730

RESUMO

Here, we demonstrated the immobilization of bacterial feruloyl esterase (FAE) from Butyrivibrio sp. XPD2006, Lactobacillus crispatus, Butyrivibrio sp. AE2015, Ruminococcus albus, Cellulosilyticum ruminicola and Clostridium cellulovorans on SBA-15 and their ability to synthesize butyl ferulate (BFA). The BFae2 from Butyrivibrio sp. XPD2006 showed the best catalytic efficiency. High BFA yield was produced when the immobilization of BFae2 took place with a high protein loading and narrow pore sized SBA-15, suggesting alteration of enzyme behavior due to the crowding environment in SBA-15. Grafting of SBA-15 with octyl moieties led to shrinking pore size and resulted in 2.5-fold increment of BFA activity compared to the free enzyme and 70%mol BFA was achieved. The BFae2 encapsulated in hydrophobic-modified SBA-15 endured up to seven reaction cycles while the BFA activity remained above 60%. This is the first report showing the superior performance of hydrophobic-modified surface to entrap FAE to produce fatty phenolic esters.

8.
Math Biosci Eng ; 16(5): 4594-4606, 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31499679

RESUMO

To explore the effects of propaganda and education on the prevention and control of AIDS infection, a model of AIDS transmission in MSM population is proposed and theoretically analyzed by introducing media impact factors. The basic reproduction number of AIDS transmission in MSM group without media intervention R0 = 1.5447 is obtained. Based on the comparison of the implementation of three different detection and treatment measures, it can be concluded that the promotion of condom use is more effective than other strategies, and using condoms with a fixed partner can reduce the value of R0 more quickly.

10.
Chemosphere ; 226: 883-890, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31509917

RESUMO

Phosphorus release is one of the disadvantages during worm predation, which has an adverse effect on wastewater treatment. In order to investigate and reveal the effects and mechanisms of worm predation on phosphorus transformation, batch experiments were conducted on a long-running worm reactor (WR). Denitrifying phosphorus removal (DPR) was observed in WR for the first time owing to the special reactor configuration and operating conditions. After DPR in WR, the concentration of supernatant phosphorus increased to 42.2 ±â€¯1.1 mg L-1 owing to bacterial phosphorus release and worm predation, which further promoted DPR in the subsequent cycle. DPR rate in the WR was 12.3 times higher than that in the blank reactor (BR). In addition, the synergistic effects of worm predation and bacterial metabolism on sludge reduction and nutrients transformation were analyzed. The sludge reduction of WR was 84.5% higher than that of BR. Bacterial metabolism played an important role in the removal of supernatant nutrients, which consumed 60.2% of total nitrogen and 55.5% of chemical oxygen demand derived from the reduced sludge. The study suggested that under certain conditions, WR could be functionalized as a bacteria selection tank to further improve the wastewater treatment efficiency. Bacterial metabolism was essential for supernatant nutrients removal during worm predation.

11.
Opt Express ; 27(16): 23103-23111, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31510592

RESUMO

We present here a detailed investigation into the sensitivity of the taper-based Mach-Zehnder interferometer as a function of external refractive index, with particular attention to the dispersion turning point (DTP) and possibilities for ultra-sensitive sensors. Our numerical simulation revealed that two DTPs exist with a decrease in the microfiber waist diameter; given this relationship, it is possible to obtain an ultra-sensitive operation. We then conducted experiments with fabricated devices with different waist diameters to achieve both positive and negative sensitivities at two DTPs. In particular, we achieved an ultrahigh refractive index sensitivity of approximately 95,832 nm/RIU at the second DTP when working with a diameter of 1.87 µm around the RI of air. These results show its potential for use in acoustic sensing and biochemical detection.

13.
Immunol Cell Biol ; 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31513306

RESUMO

Cystatin C is a ubiquitously expressed cysteine protease inhibitor that protects cells from either improper hydrolysis by endogenous proteases or pathogen growth/virulence by exogenous proteases. Although commonly used as a serum bio-marker for evaluating renal function, cystatin C is associated with many immunological disorders under various pathophysiological conditions. How cystatin C affects immune cells, especially dendritic cells (DCs), however, is far from clear. In this study, we found that pharmacological treatment with or genetic over-expression of cystatin C in bone marrow-derived DCs (BMDCs) reduced their capacity to stimulate CD4+ T cell proliferation, despite increased antigen uptake. This reduced capacity corresponded with reduced MHC-II presentation due to diminished levels of the chaperon H2-DM in BMDCs. Instead of promoting proliferation, cystatin C promoted skewing of T cells towards pro-inflammatory Th1/Th17 differentiation. This was mediated by augment Erk1/2 MAP kinase phosphorylation in BMDCs, leading to secretion of polarizing cytokines, which in turn led to the Th deviation. Collectively, our study explained the cellular and molecular basis of how this protease inhibitor can regulate immune responses, namely by affecting BMDCs and their cytokine pathway. Our results might open up an avenue for the development of therapeutic agents for the treatment of cystatin C-related immunological diseases.

14.
Sci Rep ; 9(1): 11292, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31383918

RESUMO

This study developed a novel method for the determination of 13 organophosphate esters (OPEs) in aqueous samples through the optimization of solvent demulsification-dispersive liquid-liquid microextraction based on solidification of floating organic drop procedure coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry. The proposed method was rapid and accurate and could be used in field applications. Under the most suitable conditions, the limit of detection and limit of quantification ranged from 0.16 ng/L to 20.0 ng/L and from 0.55 ng/L to 66.7 ng/L, respectively. The enrichment factors (EFs) ranged from 30 to 46. The relative standard deviations were less than 15%. The spiked recoveries ranged between 68.2% and 97.7% in the analysis of actual aqueous samples. The proposed method was convenient, environment friendly, and time and solvent saving and could be used in field applications compared with other methods. Various concentrations and types of OPEs were detected in tap water, river water, and effluent of sewage treatment plant. Effluent samples had the highest detected levels and types of OPEs.

15.
Oncol Rep ; 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31364748

RESUMO

Oral squamous cell carcinoma (OSCC), with high potential for metastasis, is the most common malignant tumor of the head and neck. Cancer­associated fibroblasts (CAFs) are the main stromal cells in the microenvironment and aggravate tumor progression. However, whether CAFs are associated with the progression of OSCC remains unknown and the underlying mechanism remains unclear. In the present study, the role of CAFs in mediating OSCC cell migration and invasion was investigated, and the participation of exosomal miR­382­5p in this process was elucidated. In this study, according to the α­SMA staining with immunohistochemistry, 47 OSCC patients were divided into CAFs­rich and CAFs poor groups, and association of CAF density and clinicopathologic features of the OSCC patients were analyzed with Pearson χ2 test. Transwell assay was used for evaluating cell migration and invasion ability of OSCC cells after being co­cultured with NFs or CAFs, or after added exosomes. qPCR was used to detect the expression of miR­382­5p. Western blot analysis was used to measure the expression of migration and invasion­associated proteins. In the present study, the CAF density in tumor tissues was found to be relevant to OSCC lymph node metastasis and TNM stage. Furthermore, we revealed that miR­382­5p was overexpressed in CAFs compared with that in fibroblasts of adjacent normal tissue and miR­382­5p overexpression was responsible for OSCC cell migration and invasion. Finally, we demonstrated that CAF­derived exosomes transported miR­382­5p to OSCC cells. The present study confirmed a new mechanism of CAF­facilitated OSCC progression and may be beneficial for identifying new cancer therapeutic targets.

16.
Biomark Med ; 13(12): 1035-1044, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31432686

RESUMO

To investigate the prevalence of EML4-ALK variants in non-small-cell lung cancer (NSCLC) patients. Materials & methods: Database of Pubmed, Embase, Medline and Cochrane Library were searched systematically to April 2018. Results: A total of 39 articles including 1903 NSCLC patients with ALK positive were recruited. The overall pooled prevalence for EML4-ALK variant 1 to 3 was 81.84% (95% CI: 76.68-86.99%), ranging from 86.64% tested by RT-PCR to 70.85% tested by other methods (p = 0.00). Subgroup analysis showed that the pooled prevalences of variant 1, 2 and 3 were 40.38% (95% CI: 34.83-45.93%), 6.59% (95% CI: 4.27-8.91%) and 26.54% (95% CI: 20.89-32.2%), respectively. Conclusion: This present study provides the exact prevalence of EML4-ALK rearrangement in different variants for NSCLC patients with ALK positive.

17.
Nat Commun ; 10(1): 3896, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31467270

RESUMO

Iron (Fe) is essential for life, but in excess can cause oxidative cytotoxicity through the generation of Fe-catalyzed reactive oxygen species. It is yet unknown which genes and mechanisms can provide Fe-toxicity tolerance. Here, we identify S-nitrosoglutathione-reductase (GSNOR) variants underlying a major quantitative locus for root tolerance to Fe-toxicity in Arabidopsis using genome-wide association studies and allelic complementation. These variants act largely through transcript level regulation. We further show that the elevated nitric oxide is essential for Fe-dependent redox toxicity. GSNOR maintains root meristem activity and prevents cell death via inhibiting Fe-dependent nitrosative and oxidative cytotoxicity. GSNOR is also required for root tolerance to Fe-toxicity throughout higher plants such as legumes and monocots, which exposes an opportunity to address crop production under high-Fe conditions using natural GSNOR variants. Overall, this study shows that genetic or chemical modulation of the nitric oxide pathway can broadly modify Fe-toxicity tolerance.

18.
Cells ; 8(9)2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31466320

RESUMO

Extracellular vesicles (EVs) contribute to a variety of signaling processes and the overall physiological and pathological states of stem cells and tissues. Human induced pluripotent stem cells (hiPSCs) have unique characteristics that can mimic embryonic tissue development. There is growing interest in the use of EVs derived from hiPSCs as therapeutics, biomarkers, and drug delivery vehicles. However, little is known about the characteristics of EVs secreted by hiPSCs and paracrine signaling during tissue morphogenesis and lineage specification. Methods: In this study, the physical and biological properties of EVs isolated from hiPSC-derived neural progenitors (ectoderm), hiPSC-derived cardiac cells (mesoderm), and the undifferentiated hiPSCs (healthy iPSK3 and Alzheimer's-associated SY-UBH lines) were analyzed. Results: Nanoparticle tracking analysis and electron microscopy results indicate that hiPSC-derived EVs have an average size of 100-250 nm. Immunoblot analyses confirmed the enrichment of exosomal markers Alix, CD63, TSG101, and Hsc70 in the purified EV preparations. MicroRNAs including miR-133, miR-155, miR-221, and miR-34a were differently expressed in the EVs isolated from distinct hiPSC lineages. Treatment of cortical spheroids with hiPSC-EVs in vitro resulted in enhanced cell proliferation (indicated by BrdU+ cells) and axonal growth (indicated by ß-tubulin III staining). Furthermore, hiPSC-derived EVs exhibited neural protective abilities in Aß42 oligomer-treated cultures, enhancing cell viability and reducing oxidative stress. Our results demonstrate that the paracrine signaling provided by tissue context-dependent EVs derived from hiPSCs elicit distinct responses to impact the physiological state of cortical spheroids. Overall, this study advances our understanding of cell‒cell communication in the stem cell microenvironment and provides possible therapeutic options for treating neural degeneration.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31466719

RESUMO

Traumatic brain injury (TBI) is a leading cause of death and disability throughout the world. However, the molecular mechanism contributing to TBI still remains unclear. Protein disulfide isomerases (PDI) are a family of redox chaperones, which catalyze formation or isomerization of disulfide bonds in proteins. PDIA3, a critical member of PDI family, is a multi-functional protein, playing critical roles in modulating inflammation, apoptosis and oxidative stress under various kinds of disease conditions. Nevertheless, its regulatory effects on TBI have far from to be known. In the present study, we attempted to explore the modulation of neuroinflammatory responses by PDIA3 and its contribution to oxidative stress and cell death after TBI in the wild type (PDIA+/+) and PDIA3 knockout (PDIA3+/+) C57BL/6 mice. Results here suggested that PDIA3 expression was markedly up-regulated in the late trauma human brain tissues, which was verified in the PDIA3+/+ mice at 24 h after TBI. PDIA-/- provided significant improvements in cognitive impairments and contusion volume induced by TBI. Apoptosis in brain samples was also alleviated in TBI mice with PDIA3 deficiency. Significantly, PDIA3-/- mitigated neuroinflammation after TBI in mice, as evidenced by the reduced expression of pro-inflammatory factors interleukin (IL)-6, tumor necrosis factor-α (TNF-α) and IL-1ß, while the enhanced anti-inflammatory regulator IL-10. These anti-inflammatory activities by PDIA3-/- were associated with the decrease in phosphorylated nuclear factor kappa B (NF-κB)/p65. PDIA3-/- mice following TBI showed attenuated oxidative stress, as proved by the restored superoxide dismutase (SOD) and glutathione (GSH) activities, and the down-regulated malondialdehyde (MDA) levels in brain samples. These effects regulated by PDIA3 were confirmed in OGDR-treated astrocytes. Collectively, these data demonstrated a detrimental role of PDIA3 in regulating TBI, providing an effective therapeutic target for TBI treatment in future.

20.
Medicine (Baltimore) ; 98(31): e16571, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31374024

RESUMO

RATIONALE: IgG4-related disease (IgG4-RD) is a systemic autoimmune disease and mixed cryoglobulinemia may be caused by autoimmune diseases. However, so far only 1 case of IgG4-RD complicated with mixed cryoglobulinemia is reported. Our case further confirms the close relationship between these 2 diseases. PATIENT CONCERNS: A 55-year-old female was admitted because of dry mouth and teeth falling off. DIAGNOSES: The patient was diagnosed as IgG4-related sialadenitis (IgG4-RS) complicated with type III mixed cryoglobulinemia. IgG4-RS was confirmed by elevated serum IgG4 levels and diffuse IgG4 plasmocyte infiltration and storiform fibrosis in the interstitium of labial gland. Type III mixed cryoglobulinemia was confirmed by positive serum cryoglobulins and no monoclonal immunoglobulin in serum and urine. INTERVENTIONS AND OUTCOMES: After treatment with prednisone and cyclophosphamide, serum cryoglobulins rapidly turned negative with the remission of IgG4-RS. LESSONS: Type III mixed cryoglobulinemia can be caused by IgG4-RS, and the underlying mechanisms need to be further explored.


Assuntos
Crioglobulinemia/complicações , Doença Relacionada a Imunoglobulina G4/complicações , Sialadenite/complicações , Crioglobulinemia/tratamento farmacológico , Feminino , Humanos , Doença Relacionada a Imunoglobulina G4/tratamento farmacológico , Imunossupressores/uso terapêutico , Pessoa de Meia-Idade , Sialadenite/tratamento farmacológico
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