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2.
Artigo em Inglês | MEDLINE | ID: mdl-36674251

RESUMO

Microtube microfilter with organic phosphoric acid (expressed as MF-OP) containing a wastewater portion with buffer fluid and an enriched portion with nitric acid fluid and organic phosphoric extractant dissolved in benzin has been studied for its trivalent holmium (expressed as Ho(III) collection from rare earth wastewater. Common parameters affecting the collection effect have been investigated, including hydrogen ion molar concentration (molar concentration can be expressed as Cm) or pH value, initial concentration (expressed as Co) of Ho(III), ion-force of rare earth wastewater, voluminal proportion of organic phosphoric extractant with benzin and nitric acid fluid (expressed as Vr), nitric acid Cm, extractant Cm, and type of acid fluid in an enriched portion. The virtues of MF-OP compared to the traditional collection was explored. The impacts of hydrodynamic characteristics (steadiness and current speed) and MF parameter factors (inradius of tube, tube-shell thickness, proportion of holes) on the collection performance of MF-OP for Ho(III) collection were also considered. The test results displayed that the greatest collection conditions of Ho(III) were attained as nitric acid Cm was 4.00 mol/L, extractant Cm was 0.220 mol/L, and Vr was 0.8 in the enriched portion, and pH value was 4.60 in the wastewater portion. Ion- force of rare earth wastewater had no noticeable outcome on Ho(III) collection. The collection proportion of Ho(III) was attainable to 93.1% in 280 min, while Co was 1.80 × 10-3 mol/L.


Assuntos
Hólmio , Metais Terras Raras , Ácido Nítrico , Solventes , Compostos Orgânicos , Ácidos , Mineração
3.
Int Immunopharmacol ; 114: 109541, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36700764

RESUMO

BACKGROUND: Exosomes exert considerable influence in mediating regulatory T (Treg) cells differentiation, which attach great importance to attenuating acute cellular rejection after liver transplantation (LT). And, miRNAs are known to play essential roles in cell-cell communication delivered by exosomes. However, the function of exosomal miRNAs in regulating Treg cells after LT remains unknown. Here, we performed an expression profiling analysis of exosome-miRNAs from human plasma after LT and investigated their immunoregulatory effects on Treg cells. METHODS: Fifty-eight LT patients and nine donors were included in this report. miRNA profiles in plasma exosomes were analyzed using next-generation sequencing. Flow cytometry, HE and multiplex immunofluorescent staining were used to identify Treg cells in the liver and peripheral blood. A lentiviral vector system was used to overexpress miR-193b-3p in dendritic cells (DCs), and exosomes isolated from these transfected cells were co-cultured with spleen lymphocytesin vitro. A quantitative Real-time PCR and enzyme-linked immunosorbent assay were used to detect the expression of cytokines. RESULTS: Treg cell infiltration was increased in the liver along with Th17 and CD8+ T cell, and it was down-regulated in peripheral blood in the acute rejection group. High-throughput sequencing revealed that miR-193b-3p was markedly up-regulated in plasma exosomes of non-rejection LT patients. The NLRP3 inflammasome was screened as a target for miR-193b-3p based on target prediction and functional enrichment analyses. Exosomal miR-193b-3p derived from DCs increased Treg cells as demonstrated in vitro. miR-193b-3p overexpression down-regulated NLRP3 as well as the inflammatory cytokines IL-1ß and IL-17A while increasing levels of the cytokines IL-10 and TGF-ß. CONCLUSION: DC derived exosomal miR-193b-3p promoted Treg cells by inhibiting NLRP3 expression. These findings not only provide a new perspective on the mechanisms, but also hold great promise for the treatment or prevention of liver allograft rejection.


Assuntos
Transplante de Fígado , MicroRNAs , Humanos , Linfócitos T Reguladores/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR , MicroRNAs/metabolismo , Fator de Crescimento Transformador beta , Células Dendríticas/metabolismo
4.
J Clin Transl Hepatol ; 11(2): 416-424, 2023 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-36643040

RESUMO

Background and Aims: Patients with biliary atresia (BA) are prone to hepatic decompensation, which might eventually lead to death. This study aimed to identify the possible risk factors affecting in-hospital death in BA patients in China. Methods: We collected data from the Hospital Quality Monitoring System, a national inpatient database. All patients aged up to 2 years old with a diagnosis of BA were included. The subjects were divided to three groups, including Kasai portoenterostomy (KP), liver transplantation (LT), and no surgery. Logistic regression with Firth's method was performed to identify potential influencing variables associated with in-hospital death. Results: During the year 2013 to 2017, there were 14,038 pediatric admissions with a diagnosis of BA. The proportion of in-hospital death in pediatric BA admissions was 1.08%. Compared with patients under six months, there was a higher risk of in-hospital death for children aged six months to 1 year and 1-2 years old. Clinical signs, including cirrhosis, variceal bleeding, and hepatic encephalopathy, were significantly associated with the risk of in-hospital death. In no surgery group, compared to those in Beijing and Shanghai, BA patients admitted in other districts had a lower risk of in-hospital death (OR=0.39, 95% CI: 0.21, 0.70). However, in the LT group, patients admitted in other districts had a higher risk of in-hospital death (OR=9.13, 95% CI: 3.99, 20.87). Conclusions: In-hospital survival remains unsatisfactory for pediatric BA patients with severe complications. Furthermore, more resources and training for BA treatment, especially LT, are essential for districts with poor medical care in the future.

5.
Inorg Chem ; 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36474312

RESUMO

In this work, a series of poly-NHC-based tetranuclear silver helicates and mesocates were synthesized from the silver-mediated self-assembly of the ligands involving multiple tridentate CNC-type pincer units and NHC coordination sites. The silver helicate was found to be transferred to a gold mesocate upon metal exchange reaction. The metallosupramolecular helicates and mesocates have been fully characterized by single-crystal X-ray crystallography, mass spectrometry, and multinuclear nuclear magnetic resonance spectroscopies. This study provides an example of the selective preparation of poly-NHC-based helicates or mesocates depending on the size of metal ions and the steric effect of ligands.

6.
Cancer Med ; 2022 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-36504294

RESUMO

AIM: This study aimed to systematically characterize transplant rejection after immune checkpoint inhibitors (ICIs) initiation in solid organ transplant recipients (SOTRs). METHODS: Data were extracted from the US FDA Adverse Event Reporting System (FAERS) database and case reports in the literature. Disproportionality analysis including information component and reported odds ratio (ROR) was performed to access potential risk signals. RESULTS: A total of 168 patients with transplant rejection after ICIs usage were identified in the FAERS database, and 89 cases were identified in the literature review. ICIs were significantly associated with transplant rejection (ROR025 : 2.2). A strong risk signal was found for combination therapy with pembrolizumab and ipilimumab compared to monotherapy. CONCLUSION: Immune checkpoint inhibitors were significantly associated with transplant rejection in SOTRs.

7.
Infect Drug Resist ; 15: 6485-6493, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36386415

RESUMO

Objective: This study aimed to investigate the effects of manual homogenization on the sensitivity of microbiological culture for patients with pyogenic spondylitis. Methods: From October 2018 to March 2021, patients undergoing fluoroscopy-guided biopsy or open debridement due to pyogenic spondylitis were recruited. Their demographic data and baseline characteristics were recorded. Tissue samples were obtained through fluoroscopy-guided biopsy or open debridement. Tissue samples were divided into three parts: manual homogenization (MH), manual mixture (MM), and pathological examination. Sterile normal saline was set as the negative control to exclude false-positive culture results. The Chi-square test was used to detect the difference of microbiological culture results. Results: Twenty-four consecutive patients (33 tissue cultures) with pyogenic spondylitis treated in our department between October 2018 and March 2021 were recruited in this study. The average age was 61.7±3.2 years old and 10 patients were female. The MH group had a significantly higher positive rate compared with the MM group in aerobic conditions: 78.8% (26 isolates) vs 54.5% (18 isolates), P=0.037 and anaerobic condition: 63.6% (21 isolates) vs 39.4% (13 isolates), P=0.049. The results of subgroup analyses showed that MH could improve the culture sensitivity for patients with previous antibiotics use and without paravertebral abscesses but not reach a significant level on statistics. Conclusion: Based on the present study, manual homogenization could improve the sensitivity of microbiological cultures for patients with pyogenic spondylitis.

9.
Biology (Basel) ; 11(11)2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36421386

RESUMO

RNA silencing is a host innate antiviral mechanism which acts via the synthesis of viral-derived small interfering RNAs (vsiRNAs). We have previously reported the infection of phytopathogenic fungi by plant viruses such as cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV). Furthermore, fungal RNA silencing was shown to suppress plant virus accumulation, but the characteristics of plant vsiRNAs associated with the antiviral response in this nonconventional host remain unknown. Using high-throughput sequencing, we characterized vsiRNA profiles in two plant RNA virus-fungal host pathosystems: CMV infection in phytopathogenic fungus Rhizoctonia solani and TMV infection in phytopathogenic fungus Fusarium graminearum. The relative abundances of CMV and TMV siRNAs in the respective fungal hosts were much lower than those in the respective experimental plant hosts, Nicotiana benthamiana and Nicotiana tabacum. However, CMV and TMV siRNAs in fungi had similar characteristics to those in plants, particularly in their size distributions, proportion of plus and minus senses, and nucleotide preference for the 5' termini of vsiRNAs. The abundance of TMV siRNAs largely decreased in F. graminearum mutants with a deletion in either dicer-like 1 (dcl1) or dcl2 genes which encode key proteins for the production of siRNAs and antiviral responses. However, deletion of both dcl1 and dcl2 restored TMV siRNA accumulation in F. graminearum, indicating the production of dcl-independent siRNAs with no antiviral function in the absence of the dcl1 and dcl2 genes. Our results suggest that fungal RNA silencing recognizes and processes the invading plant RNA virus genome in a similar way as in plants.

10.
Adv Clin Chem ; 111: 1-68, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36427907

RESUMO

Antimicrobial resistance (AMR), especially bacterial AMR, poses a global threat to public health and has become a huge obstacle to the effective control of related infectious diseases. Following the golden age of antimicrobials discovery between the 1940s and 1960s, antimicrobial abuse resulted in the rapid emergence of AMR. Nowadays, the problem of AMR has become increasingly serious, and some bacteria have reached the brink of no suitable antimicrobials available. Rapid detection of AMR and level quantification are the prerequisites to control the spread of AMR. Although time-consuming, traditional phenotype-based methods are still the primary methods used in clinical laboratories and are regarded as the gold standard for AMR identification. To offset the limitation of the long turnaround time of phenotype-based methods, molecular detection methods such as polymerase chain reaction (PCR), isothermal amplification, high-throughput sequencing, gene microarray, and mass spectrometry have begun to be widely used and served as important complements to phenotype-based methods. This chapter will describe the advances in the above technologies applied in AMR testing.


Assuntos
Anti-Infecciosos , Infecções Bacterianas , Humanos , Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Anti-Infecciosos/farmacologia , Reação em Cadeia da Polimerase
11.
Cells ; 11(22)2022 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-36429116

RESUMO

Viroids are the smallest known infectious agents that are thought to only infect plants. Here, we reveal that several species of plant pathogenic fungi that were isolated from apple trees infected with apple scar skin viroid (ASSVd) carried ASSVd naturally. This finding indicates the spread of viroids to fungi under natural conditions and further suggests the possible existence of mycoviroids in nature. A total of 117 fungal isolates were isolated from ASSVd-infected apple trees, with the majority (85.5%) being an ascomycete Alternaria alternata and the remaining isolates being other plant-pathogenic or -endophytic fungi. Out of the examined samples, viroids were detected in 81 isolates (69.2%) including A. alternata as well as other fungal species. The phenotypic comparison of ASSVd-free specimens developed by single-spore isolation and ASSVd-infected fungal isogenic lines showed that ASSVd affected the growth and pathogenicity of certain fungal species. ASSVd confers hypovirulence on ascomycete Epicoccum nigrum. The mycobiome analysis of apple tree-associated fungi showed that ASSVd infection did not generally affect the diversity and structure of fungal communities but specifically increased the abundance of Alternaria species. Taken together, these data reveal the occurrence of the natural spread of viroids to plants; additionally, as an integral component of the ecosystem, viroids may affect the abundance of certain fungal species in plants. Moreover, this study provides further evidence that viroid infection could induce symptoms in certain filamentous fungi.


Assuntos
Malus , Vírus de Plantas , Viroides , Ecossistema , Viroides/genética
13.
Emerg Microbes Infect ; 11(1): 2636-2644, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36227753

RESUMO

ABSTRACTA wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-centre retrospective study was conducted in a cohort with a history of liver transplantation. A total of 1881 participants (487 vaccinated and 1394 unvaccinated patients) were enrolled from seven centres in China. Fourteen of the participants were infected by Omicron, and 50% patients had over 14 days of viral shedding duration. The protection rate of COVID-19 vaccinations to Omicron was 2.59%. The three breakthrough infections occurred more than 6 months after fully vaccinated. A total of 96 (19.7%) vaccinated patients had adverse events, including fatigue, myalgia, liver dysfunction, swelling, and scleroma. There were more Grade 3 adverse events in the preoperative vaccination group than those in the postoperative vaccination group. Inactivated whole-virion SARS-CoV-2 vaccines are safe in patients with post-liver transplantation. The efficacy of inactivated vaccines decreases after 6 months of vaccination, it is recommended that liver transplant patients get boosted vaccinations as early as possible even when they are fully vaccinated. Although clinical manifestations of Omicron infections were mild in LT patients, unvaccinated patients might have a higher risk of liver dysfunction during infections.


Assuntos
Vacinas contra COVID-19 , COVID-19 , Transplante de Fígado , Humanos , COVID-19/prevenção & controle , Vacinas contra COVID-19/efeitos adversos , Estudos Retrospectivos , SARS-CoV-2 , Vacinação , Vacinas de Produtos Inativados/efeitos adversos
14.
Viruses ; 14(10)2022 10 17.
Artigo em Inglês | MEDLINE | ID: mdl-36298833

RESUMO

Investigating a virus's host range and cross-infection is important for better understanding the epidemiology and emergence of viruses. Previously, our research group discovered a natural infection of a plant RNA virus, cumber mosaic virus (genus Cucumovirus, family Bromoviridae), in a plant pathogenic basidiomycetous fungus, Rhizoctonia solani, isolated from a potato plant grown in the field. Here, we further extended the study to investigate whether similar cross-infection of plant viruses occurs widely in plant-associated fungi in natural conditions. Various vegetable plants such as spinach, leaf mustard, radish, celery, and other vegetables that showed typical virus-like diseases were collected from the fields in Shandong Province, China. High-throughput sequencing revealed that at least 11 known RNA viruses belonging to different genera, including Potyvirus, Fabavirus, Polerovirus, Waikavirus, and Cucumovirus, along with novel virus candidates belonging to other virus genera, infected or associated with the collected vegetable plants, and most of the leaf samples contained multiple plant viruses. A large number of filamentous fungal strains were isolated from the vegetable leaf samples and subjected to screening for the presence of plant viruses. RT-PCR and Sanger sequencing of the PCR products revealed that among the 169 fungal strains tested, around 50% were carrying plant viruses, and many of the strains harbored multiple plant viruses. The plant viruses detected in the fungal isolates were diverse (10 virus species) and not limited to particular virus genera. However, after prolonged maintenance of the fungal culture in the laboratory, many of the fungal strains have lost the virus. Sequencing of the fungal DNA indicated that most of the fungal strains harboring plant viruses were related to plant pathogenic and/or endophytic fungi belonging to the genera Alternaria, Lecanicillium, and Sarocladium. These observations suggest that the nonpersistent acquisition of plant viruses by fungi may commonly occur in nature. Our findings highlight a possible role for fungi in the life cycle, spread, and evolution of plant viruses.


Assuntos
Cucumovirus , Vírus de Plantas , Potyvirus , DNA Fúngico , Doenças das Plantas , Plantas , RNA de Plantas
15.
J Transl Med ; 20(1): 479, 2022 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-36266691

RESUMO

BACKGROUND: Explanted livers from patients with inherited metabolic liver diseases possess the potential to be a cell source of good-quality hepatocytes for hepatocyte transplantation (HT). This study evaluated the therapeutic effects of domino HT using hepatocytes isolated from explanted human livers for acute liver failure (ALF). METHODS: Isolated hepatocytes were evaluated for viability and function and then transplanted into D-galactosamine/lipopolysaccharide-induced ALF mice via splenic injection. The survival rate was analyzed by the Kaplan-Meier method and log-rank test. Liver function was evaluated by serum biochemical parameters, and inflammatory cytokine levels were measured by ELISA. The pathological changes in the liver tissues were assessed by hematoxylin-eosin staining. Hepatocyte apoptosis was investigated by TUNEL, and hepatocyte apoptosis-related proteins were detected by western blot. The localization of human hepatocytes in the injured mouse livers was detected by immunohistochemical analyses. RESULTS: Hepatocytes were successfully isolated from explanted livers of 10 pediatric patients with various liver-based metabolic disorders, with an average viability of 85.3% ± 13.0% and average yield of 9.2 × 106 ± 3.4 × 106 cells/g. Isolated hepatocytes had an excellent ability to secret albumin, produce urea, uptake indocyanine green, storage glycogen, and express alpha 1 antitrypsin, albumin, cytokeratin 18, and CYP3A4. Domino HT significantly reduced mortality, decreased serum levels of alanine aminotransferase and aspartate aminotransferase, and improved the pathological damage. Moreover, transplanted hepatocytes inhibited interleukin-6 and tumor necrosis factor-α levels. Domino HT also ameliorates hepatocyte apoptosis, as evidenced by decreased TUNEL positive cells. Positive staining for human albumin suggested the localization of human hepatocytes in ALF mice livers. CONCLUSION: Explanted livers from patients with inheritable metabolic disorders can serve as a viable cell source for cell-based therapies. Domino HT using hepatocytes with certain metabolic defects has the potential to be a novel therapeutic strategy for ALF.


Assuntos
Hepatócitos , Falência Hepática Aguda , Doenças Metabólicas , Animais , Criança , Humanos , Camundongos , Alanina Transaminase/metabolismo , Albuminas/metabolismo , alfa 1-Antitripsina/metabolismo , Aspartato Aminotransferases/metabolismo , Citocromo P-450 CYP3A/metabolismo , Galactosamina/efeitos adversos , Glicogênio/metabolismo , Interleucina-6/metabolismo , Queratina-18/metabolismo , Lipopolissacarídeos , Falência Hepática Aguda/induzido quimicamente , Falência Hepática Aguda/cirurgia , Doenças Metabólicas/induzido quimicamente , Doenças Metabólicas/cirurgia , Albumina Sérica Humana/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Ureia/metabolismo , Hepatócitos/transplante
16.
Front Immunol ; 13: 944898, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36148220

RESUMO

Background: Actin-related protein 2/3 complex subunit 5 (ARPC5) is one of the members of actin-related protein 2/3 complex and plays an important role in cell migration and invasion. However, little is known about the expression pattern, prognosis value, and biological function of ARPC5 in pan-cancer. Thus, we focus on ARPC5 as cut point to explore a novel prognostic and immunological biomarker for cancers. Methods: The public databases, including TCGA, GTEx, and UCEC, were used to analyze ARPC5 expression in pan-cancer. The Human Protein Atlas website was applied to obtain the expression of ARPC5 in different tissues, cell lines, and single-cell types. Univariate Cox regression analysis and Kaplan-Meier analysis were used to explore the prognosis value of ARPC5 in various cancers. Spearman's correlation analysis was performed to investigate the association between ARPC5 expression and tumor microenvironment scores, immune cell infiltration, immune-related genes, TMB, MSI, RNA modification genes, DNA methyltransferases, and tumor stemness. Moreover, qPCR, Western blot, and immunohistochemistry were carried out to examine the differential expression of ARPC5 in HCC tissues and cell lines. CCK8, EdU, flow cytometry, wound-healing assays, and transwell assays were conducted to explore its role in tumor proliferation, apoptosis, migration, and invasion among HCC cells. Results: ARPC5 expression was upregulated in most cancer types and significantly associated with worse prognosis in KIRC, KIRP, LGG, and LIHC. mRNA expression of ARPC5 showed low tissue and cell specificity in normal tissues, cell lines, and single-cell types. ARPC5 expression was positively correlated with the tumor microenvironment scores, immune infiltrating cells, immune checkpoint-related genes in most cancers. ARPC5 in STAD and BRCA was positively associated with TMB, MSI, and neoantigens. We also discovered that ARPC5 was correlated with the expression of m1A-related genes, m5C-related genes, m6A-related genes, and DNA methyltransferases. In experiment analyses, we found that ARPC5 was significantly highly expressed in HCC tissues and HCC cells. Functionally, silencing ARPC5 dramatically decreased proliferation, migration, and invasion ability of HCC cells. Conclusions: ARPC5 expression affects the prognosis of multiple tumors and is closely correlated to tumor immune infiltration and immunotherapy. Furthermore, ARPC5 may function as an oncogene and promote tumor progression in HCC.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Proteína 2 Relacionada a Actina/genética , Proteína 2 Relacionada a Actina/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , DNA , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/patologia , Metiltransferases/genética , Prognóstico , RNA , RNA Mensageiro/genética , Microambiente Tumoral/genética
18.
Sci Total Environ ; 853: 158643, 2022 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-36089042

RESUMO

There is recognition that biochar addition is an appropriate measure to mitigate methane (CH4) emissions by promoting potential methane oxidation (PMO) in the field. However, the mechanism for different field-aged biochars and effective duration after field application are not well documented. Based on a long-term field experiment, biochar was field aged and separated from two contrasting acidic (Ba) and saline (Bs) paddy fields. Then, the effects of different aged biochars on PMO in acidic and saline paddy soils were explored by incubation experiment. There were five treatments for each soil group: soil without biochar (CK), biochar-enriched paddy soil (2 or 6 years) (NB), fresh biochar amendment (Bf), aged biochar separated from acidic paddy soil amendment (Ba), and aged biochar separated from saline paddy soil amendment (Bs). Results showed that saline paddy soils had a significantly higher PMO than acidic paddy soils under treatment without biochar, and that PMO in acidic paddy soil was enhanced by various biochar amendments, whereas those biochar amendments had no significant effects on PMO in saline paddy soil. PMO was positively correlated with pmoA abundance, N consumption rate and pH of soil-biochar mixture. Aged biochar separated from different fields had conflicting influences on soil pH, N consumption rate and PMO. Ba lost its initial effect on changing PMO as compared to Bf treatment when added back into acidic paddy soil. To the contrary, the acidic paddy soil NB treatment containing biochar added six years before possessed the highest value of PMO among all ten treatments. This study suggested that acidic paddy soil with biochar amendment could mitigate CH4 emissions by promoting PMO for a prolonged period, though aged biochar separated from the same field had a limited impact on reducing CH4 emissions.


Assuntos
Oryza , Solo , Metano , Carvão Vegetal
19.
Toxics ; 10(9)2022 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-36136511

RESUMO

A novel Di-phase membrane device with DZ272 (DDD) containing a replenishing feed section and replenishing resolving section for the purification behavior of Co(II) has been studied. The replenishing feed section was composed of feed solution and Di-isooctylphosphinic acid (DZ272) as the carrier in fossil oil, and the replenishing resolving section was composed of DZ272 as the carrier in fossil oil and HCl as the resolving agent. The effects of the voluminal ratio of the membrane solution and feed solution (O/F), pH, initial molarity of Co(II) and ionic strength in the feed solution, voluminal ratio of membrane solution and resolving agent (O/S), molarity of H2SO4 solution and DZ272 molarity in the replenishing resolving section on purification of Co(II) were considered. The benefits of DDD compared to the traditional membrane device, system stability, reuse of the membrane solution and retention of the membrane section were also studied. Experimental results indicated that the optimal purification conditions of Co(II) were obtained, as H2SO4 molarity was 2.00 mol/L, DZ272 molarity was 0.120 mol/L, O/S was 3:1 in the replenishing resolving section, O/F was 1:8 and pH was 5.20 in the replenishing feed section. The ions intensity in the replenishing feed section had no apparent effect on purification behavior of Co(II). When the initial Co(II) molarity was 3.00 × 10-4 mol/L, the purification percentage of Co(II) achieved 93.6% in 200 min. The kinetic equation was deduced in light of the law of mass diffusivity and interfacial chemistry.

20.
Eur J Pharmacol ; 934: 175294, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36152840

RESUMO

Toll-like receptor 4, a highly conserved protein of innate immunity, is responsible for the regulation and maintenance of homeostasis. It has been implicated in the progression of ulcerative colitis (UC) by interacting with its downstream pathway myeloid differentiation factor 88 (MyD88) and nuclear factor kappa B (NF-κB). This study aimed to evaluate the effect of a specific inhibitor of toll-like receptor 4, TLR4-IN-C34 on gut microbiota to elucidate its mechanism in UC mice. Dextran sulfate sodium significantly induced weight loss, diarrhea and rectal bleeding, and colonic damage in mice, which occurred concomitant with dysbiosis of intestinal flora. Intestinal dysbiosis were partially ameliorated by TLR4-IN-C34. Meanwhile, a reduction in inflammatory cell infiltration, enhanced antioxidant activity in colon tissues, and reconstruction of intestinal barrier were observed in mice administrated with TLR4-IN-C34. MyD88 and NF-κB were significantly reduced after TLR4-IN-C34 treatment. MyD88-/- mice were found with improved dysbiosis of intestinal flora, which was mitigated by overexpression of NF-κB. Collectively, our results suggest that TLR4-IN-C34 alleviates UC in mice by blocking the MyD88/NF-κB pathway to improve intestinal flora dysbiosis, inflammatory infiltration, oxidative stress and intestinal barrier function.


Assuntos
Colite Ulcerativa , Colite , Microbioma Gastrointestinal , Camundongos , Animais , Fator 88 de Diferenciação Mieloide/metabolismo , Receptor 4 Toll-Like/metabolismo , NF-kappa B/metabolismo , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Sulfato de Dextrana , Disbiose , Antioxidantes/farmacologia , Transdução de Sinais , Homeostase
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