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1.
Radiother Oncol ; 2021 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-33621587

RESUMO

BACKGROUND AND PURPOSE: To compare 68Ga-fibroblast activation protein inhibitor (FAPI) and 18F-FDG PET/CT in imaging locally advanced oesophageal cancer, and evaluate the potential usefulness of 68Ga-FAPI PET/CT on gross target volume (GTV) delineation aimed at radiotherapy planning for oesophageal cancer as compared with contrast-enhanced CT (CE-CT) and 18F-FDG PET/CT. MATERIALS AND METHODS: Twenty-one patients with newly diagnosed oesophageal cancer who underwent both 18F-FDG and 68Ga-FAPI PET/CT scans were selected. GTVs of the primary tumours based on CE-CT (GTVCT), PET/CT, and CE-CT plus PET/CT were delineated. Gross tumour lengths were measured by GTVs and endoscopy and recorded. RESULTS: The 68Ga-FAPI PET showed significantly higher radiotracer uptake than 18F-FDG PET (median SUVmax 16.71 vs. 11.23; P=0.002) in the primary tumours. SUV thresholds of FAPI ×20%, 30%, 40%, and FDG ×40% showed similar lesion lengths compared with that in endoscopic examination (P>0.05). GTVCT demonstrated the largest volume (median: 48.80 mm3, range: 14.83-162.23 mm3) than PET-based GTVs. For PET/CT-guided complementary contouring of GTVCT, four patients (19%) were increased by FAPI ×20% and 30%, two patients (9.5%) were increased by FAPI ×40%, and only one patient was increased by FDG ×40%. Furthermore, the volume of GTV based on CE-CT plus FAPI ×20%, 30%, and 40% showed no significant difference with GTVCT and planning target volume based CE-CT plus FAPI-PET and meets the organ at risk standard. CONCLUSION: The 68Ga-FAPI PET/CT methodology showed favourable tumour-to-background contrast in oesophageal cancer and might provide additional information for target volume delineation and help avoid tumour geographic misses.

2.
Nat Prod Res ; : 1-6, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33517796

RESUMO

Two new 2,5-diketopiperazines derivatives (1-2), together with eight known analogs (3-10), were isolated from a culture broth of an endophytic fungus Nigrospora camelliae-sinensis S30, derived from mangrove Lumnitzera littorea. Their complete structures were determined by a detailed analysis of spectroscopic data and ECD calculations. The antimicrobial activity and neuroprotective activity of these isolated compounds were also evaluated.

3.
Biomed Pharmacother ; 136: 111241, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33485069

RESUMO

Previously, we have demonstrated that policosanol from Chinese wax suppressed testosterone(T)-induced alopecia in mice. However, the underlying mechanism remained to be determined. Herein, we investigated the mechanism of policosanol against androgenetic alopecia (AGA). AGA was induced in Kunming mice by subcutaneous administration of testosterone propionate for 60 d. Policosanol (0.5 %, 1% or 2%) was applied topically on the back of mice. Finasteride (2%) was applied topically as a positive control. The serum T and estradiol (E2) concentrations were determined by ELISA after 28 and 60 days of treatment. The cutaneous expression or activity of key mediators of hair growth, such as alkaline phosphatase (ALP), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF), was measured. MTS assay was performed to evaluate cell proliferation in cultured human dermal papilla cells (DPCs) treated with dihydrotestosterone (DHT). Western blotting was performed to evaluate the protein expression of Bax, Bcl2, TGF-ß2, caspase-9, and caspase-3. We found lower T and T/E2 ratio in mice treated with policosanol than in the model group. Policosanol suppressed premature hair follicle entry into the regression phase, as shown by improving VEGF and EGF expression and ALP activity. The MTS assay showed that policosanol markedly inhibited the apoptosis of DHT-treated DPCs. Western blotting showed that policosanol significantly reduced the protein expression of TGF-ß2, cleaved caspese-9, cleaved caspase-3, and Bax, and increased that of Bcl2. The optimal effect was obtained with 12.50 g/mL policosanol. In conclusion, policosanol prevents androgenetic alopecia by regulating hormone levels and suppressing premature hair follicle entry into the regression phase.

4.
PLoS One ; 16(1): e0245843, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33493183

RESUMO

BACKGROUND: Hazard perception ability, which develops with driving experience, has been proven to be associated with drivers' traffic involvement. Although classic reaction time-based hazard perception tests have been developed in many developed counties, experience-related differences may not be found in drivers from developing countries due to their increased opportunities to experience hazards on roads. Therefore, the present study aims to develop a hazard prediction test for Chinese drivers based on a predictive paradigm called "What happens next?" and assess its reliability and validity. METHOD: Thirty-six video clips filmed from drivers' perspectives of Chinese driving settings were presented to 54 novice drivers and 47 experienced drivers. Participants were asked to answer three questions after each video clip was blacked out and to then quickly press the mouse button on a reaction time-based hazard perception test. Both the differences in the test scores between novice and experienced drivers and the differences in scores between drivers with and without traffic violations were compared. RESULTS: The final hazard prediction test consisted of 20 video clips. A high internal consistency coefficient of the test, i.e., Cronbach's alpha = 0.862, was obtained. The total scores of the test were positively and significantly correlated with reaction times as measured on the video-based hazard perception test, thus providing evidence regarding the discriminant validity of the test. More importantly, drivers with traffic violations obtained significantly lower total scores on the test than did drivers without traffic violations. CONCLUSION: The newly developed hazard prediction test exhibited adequate psychometric properties and provided a practical alternative for assessing drivers' hazard perception ability in China.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33415432

RESUMO

PURPOSE: The aim of this study was to explore the role of [68Ga]Ga-DOTA-FAPI-04 positron emission tomography/computed tomography (PET/CT), compared with 18F-fluorodeoxyglucose [18F]-FDG PET/CT, for evaluating peritoneal carcinomatosis in patients with various types of cancer. METHODS: Patients with suspected peritoneal malignancy, who underwent both [18F]-FDG and [68Ga]Ga-DOTA-FAPI-04 PET/CT between October 2019 and August 2020, were retrospectively analysed. The radiotracer uptake, peritoneal cancer index (PCI) score, and diagnostic performance of [18F]-FDG and [68Ga]Ga-DOTA-FAPI-04 PET/CT were evaluated and compared. RESULTS: Our cohort consisted of 46 patients, including 16 patients with diffuse-type peritoneal carcinomatosis, 27 with nodular-type peritoneal carcinomatosis, and 3 true-negative patients. A significant difference in standard uptake values (SUV) of lesions between [18F]-FDG and [68Ga]Ga-DOTA-FAPI-04 PET/CT examination was observed (median SUV: 3.48 vs. 9.82; P < 0.001), particularly in peritoneal carcinomatosis from gastric cancer (median SUV: 3.44 vs. 8.05; P = 0.001). Moreover, [68Ga]Ga-DOTA-FAPI-04 PET/CT showed a higher PCI score and better sensitivity than [18F]-FDG PET/CT for the detection of peritoneal carcinomatosis (6 vs. 18; P < 0.001; 72.09% vs. 97.67%; P = 0.002). CONCLUSION: [68Ga]Ga-DOTA-FAPI-04 PET/CT demonstrated superior sensitivity over [18F]-FDG PET/CT for the detection of peritoneal carcinomatosis in patients with various types of cancer, particularly gastric cancer. Furthermore, the uptake of [68Ga]Ga-DOTA-FAPI-04 in peritoneal carcinomatosis was significantly higher than that of [18F]-FDG, demonstrating a larger extent of the lesions and yielding a higher PCI score. This could help enhance the image contrast, improve physicians' diagnostic confidence, and reduce the proportion of missed diagnoses.

6.
J Affect Disord ; 278: 506-514, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33017678

RESUMO

BACKGROUND: Increasing evidence demonstrates the significant role of weight perception in shaping the mental health of adolescents. This study sought to extend previous literature by examining the associations of weight perception categories with depressive symptoms for both boys and girls in China, and further explored whether the associations varied between urban and rural areas. METHODS: Data were from a large-scale, national survey of Chinese middle school students. The analytical sample had 8568 respondents with a mean age of 13.9 years (SD=0.7). Bivariate and multiple regression analyses were adopted to examine the links between weight perception and depressive symptoms. RESULTS: Overweight perceptions were more prevalent in girls than in boys (47.0% vs. 29.3%), whereas underweight perceptions were less common in girls than in boys (15.7% vs. 29.3%). After adjusting for sociodemographic characteristics and health conditions, self-perceiving as "slightly overweight" and "very overweight" were significantly related to more depressive symptoms for both boys (p<0.05) and girls (p<0.05). A "very underweight" perception, however, was found to be only linked to greater depressive symptoms in boys (p<0.05). Notably, these patterns showed no significant rural-urban difference, as evidenced by the lack of significant interactive effects of all weight perceptions categories and region (urban/rural) on depressive symptoms. LIMITATIONS: This study was based on a cross-sectional design, which essentially precludes the possibility to determine the causality of the observed associations. CONCLUSION: Findings in this study indicated that the impacts of subjective physical attractiveness have been gendered and regionally pervasive in affecting emotional well-being of Chinese adolescents.

7.
J Environ Sci (China) ; 99: 196-209, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33183697

RESUMO

The submicron particulate matter (PM1) and fine particulate matter (PM2.5) are very important due to their greater adverse impacts on the natural environment and human health. In this study, the daily PM1 and PM2.5 samples were collected during early summer 2018 at a sub-urban site in the urban-industrial port city of Tianjin, China. The collected samples were analyzed for the carbonaceous fractions, inorganic ions, elemental species, and specific marker sugar species. The chemical characterization of PM1 and PM2.5 was based on their concentrations, compositions, and characteristic ratios (PM1/PM2.5, AE/CE, NO3-/SO42-, OC/EC, SOC/OC, OM/TCA, K+/EC, levoglucosan/K+, V/Cu, and V/Ni). The average concentrations of PM1 and PM2.5 were 32.4 µg/m3 and 53.3 µg/m3, and PM1 constituted 63% of PM2.5 on average. The source apportionment of PM1 and PM2.5 by positive matrix factorization (PMF) model indicated the main sources of secondary aerosols (25% and 34%), biomass burning (17% and 20%), traffic emission (20% and 14%), and coal combustion (17% and 14%). The biomass burning factor involved agricultural fertilization and waste incineration. The biomass burning and primary biogenic contributions were determined by specific marker sugar species. The anthropogenic sources (combustion, secondary particle formation, etc) contributed significantly to PM1 and PM2.5, and the natural sources were more evident in PM2.5. This work significantly contributes to the chemical characterization and source apportionment of PM1 and PM2.5 in near-port cities influenced by the diverse sources.


Assuntos
Poluentes Atmosféricos , Aerossóis/análise , Poluentes Atmosféricos/análise , Biomassa , China , Cidades , Monitoramento Ambiental , Humanos , Material Particulado/análise , Estações do Ano , Emissões de Veículos/análise
8.
Radiology ; : 203275, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33258746

RESUMO

Background Accurate clinical staging is crucial to managing gastrointestinal cancer, but fluorine 18 (18F) fluorodeoxyglucose (FDG) PET/CT has limitations. Targeting fibroblast-activation protein is a newer diagnostic approach for the visualization of tumor stroma, and gallium 68 (68Ga)-labeled fibroblast-activation protein inhibitors (FAPIs), hereafter 68Ga-FAPIs, present a promising alternative to 18F-FDG. Purpose To compare the diagnostic efficacy of 68Ga-FAPI PET/CT in primary and metastatic lesions of gastrointestinal malignancies with that of 18F-FDG PET/CT. Materials and Methods Images from patients with gastric, duodenal, and colorectal cancers who underwent contemporaneous 18F-FDG and 68Ga-FAPI PET/CT between October 2019 through June 2020 were retrospectively analyzed. 18F-FDG and 68Ga-FAPI uptakes were compared by using the Wilcoxon signed-rank test. The McNemar test was used to compare the diagnostic performance between the two techniques. Results Thirty-five patients (median age, 64 years [interquartile range, 53-68 years]; 18 men) were evaluated. In treatment-naive patients (n = 19), 68Ga-FAPI PET/CT led to upstaging of the clinical TNM stage in four (21%) patients compared with 18F-FDG PET/CT. Tracer uptake was higher with 68Ga-FAPI PET/CT than with 18F-FDG PET/CT in primary lesions (gastric cancer: 12.7 vs 3.7, respectively, P = .003; colorectal cancer: 15.9 vs 7.9, P = .03), involved lymph nodes (6.7 vs 2.4, P < .001), and bone and visceral metastases (liver metastases: 9.7 vs 5.2, P < .001; peritoneal metastases: 8.4 vs 3.6, P < .001; bone metastases: 4.3 vs 2.2, P < .001; lung metastases: 4.4 vs 1.9, P = .01). In addition, the sensitivity of 68Ga-FAPI PET/CT was higher than that of 18F-FDG PET/CT in the detection of primary tumors (100% [19 of 19] vs 53% [10 of 19], respectively; P = .004), lymph nodes (79% [22 of 28] vs 54% [15 of 28], P < .001), and bone and visceral metastases (89% [31 of 35] vs 57% [20 of 35], P < .001). Conclusion Gallium 68 fibroblast-activation protein inhibitor PET/CT was superior to fluorine 18 fluorodeoxyglucose PET/CT in the detection of primary and metastatic lesions in gastric, duodenal, and colorectal cancers, with higher tracer uptake in most primary and metastatic lesions. Published under a CC BY 4.0 license.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33196860

RESUMO

OBJECTIVE: Workplace violence has been recognized globally as a serious occupational hazard in health service occupations, and existing studies have identified that workplace violence can significantly lead to depression. Interpersonal distrust, an important topic, has also been proved associated with workplace violence and depression. However, the mediating effect of interpersonal distrust has not been tested before. Results of such testing can help us to understand further the effect mechanism of workplace violence on depression. METHODS: In the current study, we collected 3426 valid questionnaires based on a cross-sectional design distributed among medical staff in Chinese hospitals. Depression, workplace violence, interpersonal distrust, social support, physical diseases, and some other social-demographic variables were evaluated. SPSS macros program (PROCESS v3.3) was used to test the mediating effect of interpersonal distrust on the association between workplace violence and depression. RESULTS: The data analyzed in the current study demonstrated that 52.2% of medical staff had experienced workplace violence before. Experiencing verbal violence (ß = 2.99, p < 0.001), experiencing physical violence (ß = 3.70, p < 0.01), experiencing both kinds of violence (ß = 4.84, p < 0.001), high levels of interpersonal distrust (ß = 0.22, p < 0.001), working as a nurse (ß = 1.10, p < 0.05), working as a manager (ß = - 1.72, p < 0.001), suffering physical disease (ß = 3.35, p < 0.001), and receiving social support (ß = - 0.23, p < 0.001) were significantly associated with depression. Workplace violence had not only positive direct effects on depression, but also an indirect effect on depression through interpersonal distrust as a mediator. CONCLUSION: Interpersonal distrust can mediate the association between workplace violence and depression. Increasing interpersonal trust or reducing workplace violence would be beneficial to promoting mental health status among medical staff.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33179149

RESUMO

PURPOSE: This study aimed to evaluate the potential utility of [68Ga]Ga-FAPI-04 PET/CT for diagnosing primary and metastatic lesions in patients with liver cancer, as well as to compare it with contrast-enhanced CT (CE-CT), liver MRI, and [18F]-FDG PET/CT. METHODS: We performed a single-center post hoc retrospective analysis of data obtained from a prospective parent study (NCT04416165). This study included 34 patients diagnosed with or suspected hepatic lesions who underwent concomitant [68Ga]Ga-FAPI-04 and [18F]-FDG/CT scans. Moreover, these patients underwent liver MRI (n = 34) and CE-CT (n = 25). Histopathologic (n = 62) or radiographic follow-up (n = 128) served as the reference standard for the final diagnosis. RESULTS: Among the 34 patients, 20, 12, and 2 patients presented with hepatocellular carcinomas, intrahepatic cholangiocarcinomas, and benign hepatic nodules, respectively. The sensitivities of CE-CT, MRI, [68Ga]Ga-FAPI-04, and [18F]-FDG/CT for detecting primary liver tumors were 96%, 100%, 96%, and 65%, respectively. Regarding the diagnosis of all intrahepatic lesions, the per-lesion detection rate of [68Ga]Ga-FAPI-04 PET/CT was slightly lower than that of MRI (85% vs. 100%, P = 0.34) and significantly higher than that of [18F]-FDG PET/CT (85% vs. 52%, P < 0.001). Regarding the diagnosis of all malignant lesions (including extrahepatic disease), the tumor detection rate of [68Ga]Ga-FAPI-04 PET/CT was 87.4%, which was significantly higher than that of [18F]-FDG PET/CT (65.0%, P < 0.001). CONCLUSIONS: Our findings indicate that the sensitivity of [68Ga]Ga-FAPI-04 PET/CT to correctly identify primary liver tumors and metastatic lesions is equivalent to that of CE-CT and liver MRI. Moreover, [68Ga]Ga-FAPI-04 PET/CT is better at identifying liver lesions than [18F]-FDG PET/CT, and its use may improve tumor staging, recurrence detection, and implementation of necessary treatment modifications.

11.
PLoS One ; 15(11): e0242374, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33211732

RESUMO

The present study aimed to adapt the Attitudes Toward Accompanied Driving Scale (ATADS) to a Chinese drivers sample and to examine its reliability and validity. Five hundred and seventy-two drivers aged 18 to 25 years old were asked to complete the ATADS and a validated Chinese version of the Multidimensional Driving Style Inventory. The factorial structure of the ATADS was examined using exploratory factor analysis (N = 259) and confirmatory factor analysis (N = 313). The validity of the scale was evaluated by examining the associations between the ATADS factors, demographic variables and driving styles. The results showed that both the findings of the EFA and CFA showed a five-factor structure of the ATADS, including tension, relatedness, avoidance, disapproval and anxiety. Second, significant gender differences were found in tension, relatedness, avoidance and anxiety. Third, tension, avoidance, disapproval and anxiety were moderately or weakly correlated with risky, anxious, angry and careful driving styles. Moreover, the number of traffic accidents after the accompanying phase was positively correlated with disapproval and avoidance. The findings supported the psychometric properties of the Chinese version of the ATADS and highlighted the adverse effects of young drivers' negative attitudes toward accompanied driving on their driving styles.

12.
Mol Med Rep ; 22(6): 5313-5325, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33174011

RESUMO

Osteoarthritis is a chronic degenerative joint disease. Long non­coding RNA plasmacytoma variant translocation 1 (PVT1) is involved in the progression of osteoarthritis and exosomes serve a central role in intercellular communication. However, whether PVT1 can be mediated by exosomes in osteoarthritis has not been reported. Whole blood was drawn from osteoarthritis patients and healthy volunteers. Lipopolysaccharide (LPS) was used to stimulate human normal chondrocytes (C28/I2) to construct a cell damage model in vitro. Protein levels were examined via western blot analysis. eThe expression of PVT1, microRNA (miR)­93­5p and high mobility groupprotein B1 (HMGB1) was evaluated through reverse transcription­quantitative PCR. Cell viability and apoptosis were determined through CCK­8 or flow cytometric assay. Inflammatory cytokines were measured via ELISA. The relationship between PVT1 or HMGB1 and miR­93­5p was confirmed by dual­luciferase reporter assay. PVT1, HMGB1 and exosomal PVT1 were upregulated while miR­93­5p was downregulated in osteoarthritis patient serum and LPS­induced C28/I2 cells. Exosomes from osteoarthritis patient serum and LPS­treated C28/I2 cells increased PVT1 expression in C28/I2 cells. PVT1 depletion reversed the decrease of viability and the increase of apoptosis, inflammation responses and collagen degradation of C28/I2 cells induced by LPS. PVT1 regulated HMGB1 expression via sponging miR­93­5p. miR­93­5p inhibition abolished PVT1 silencing­mediated viability, apoptosis, inflammation responses and collagen degradation of LPS­stimulated C28/I2 cells. HMGB1 increase overturned miR­93­5p upregulation­mediated viability, apoptosis, inflammation responses and collagen degradation of LPS­stimulated C28/I2 cells. Furthermore, PVT1 modulated the Toll­like receptor 4/NF­κB pathway through an miR­93­5p/HMGB1 axis. In summary, exosome­mediated PVT1 regulated LPS­induced osteoarthritis progression by modulating the HMGB1/TLR4/NF­κB pathway via miR­93­5p, providing a new route for possible osteoarthritis treatment.

13.
Front Vet Sci ; 7: 520, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33088823

RESUMO

Based on data from three major pig diseases, this study calculated the animal disease epidemic index of 31 provinces and autonomous regions in mainland China. We adopted the Gini coefficient to investigate the interregional differences in animal disease epidemic risk and used the Shapley value decomposition method to illustrate the contribution of influencing factors. The results showed that the Gini coefficient remains above 0.60, indicating significant interregional differences in mainland China. Animal breeding level, ecological environment, and animal disease prevention and control contribute most to the interregional differences in animal epidemic risk. The results imply that reducing sewage discharge, increasing pig production, and changing the breeding style from free-range to large-scale farming are measures that may help improve disease prevention and control. This study has implications for providing theoretical references for preventing and controlling animal epidemics and for improving public health governance.

14.
Dalton Trans ; 49(40): 13958-13961, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33021307

RESUMO

Discrete supertetrahedral clusters of metal chalcogenides are rare because of the difficulty involved in meeting global charge matching between the negative charge of the skeleton and counterion. We present herein the third type of a discrete chalcogenide cluster with a double T3 structure in the compound (HDBN)6[In20S33(DBN)6] (DBN = 1,5-diazabicyclo [4.3.0]-5-nonene), the anion of which features quasi-D3 symmetrical double-T3 In20S33 supertetrahedra with six cornered indium atoms coordinated by DBN molecules. DFT theory calculations of the interaction between host and guest show that this compound may have high kinetic stability and low photoelectric reactivity.

15.
Clin Lab ; 66(9)2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32902209

RESUMO

BACKGROUND: Breast tumor is a common cancer in women all over the world. Long noncoding RNA (lncRNA) provides a significant and new perspective on understanding biomarkers as well as on the potential prognostic regulation of breast cancer. Its transcription, in turn, serves as a regulator in diagnosing breast cancer and preventing risk of recurrence. Here, we review the evolution of lncRNAs and discuss their regulative roles in the metastasis of breast cancer. Moreover, we aim to detect the expression level of lncRNA HOTAIR in different stages of breast cancer. METHODS: Sixty patients with breast cancer at different stages were divided into four groups based on different stages. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression level of lncRNA HOTAIR in breast tumor tissue. RESULTS: Compared to stage I breast cancer, the expression profiles of lncRNA HOTAIR in stage II, III, IV breast cancer are significantly elevated (p < 0.05). The expression profiles of lncRNA HOTAIR in stage III and IV breast cancer are significantly increased compared with stage II breast cancer. CONCLUSIONS: Consistent with microRNAs (miRNA), lncRNAs could function as underlying effective biomarkers to affect the biogenesis and gene control across all lifetime. The interaction between lncRNA and miRNA plays a crucial role in the metastasis of breast cancer and provides a potential biomarker target for breast cancer metastasis therapy. Our study has also demonstrated that the expression profiles of lncRNA HOTAIR in stage II, III, IV breast cancer are significantly elevated.

16.
Ecotoxicol Environ Saf ; 203: 111031, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32888610

RESUMO

Bone mineral density (BMD) changes were reported to be associated with excessive fluoride exposure and abnormal expression of RUNX2. However, whether the alteration of methylation status, a most commonly used marker for the alteration of gene expression in epidemiological investigation, of RUNX2 is associated with low-to-moderate fluoride exposure and BMD changes has not been reported. Our study aims to explore the role of RUNX2 promoter methylation in BMD changes induced by low-to-moderate fluoride exposure. A total of 1124 adults (413 men and 711 women) were recruited from Kaifeng City in 2017. We measured BMD using ultrasound bone densitometer. Concentrations of urinary fluoride (UF) were measured using ion-selective electrode, and the participants were grouped into control group (CG) and excessive fluoride group (EFG) according to the concentration of UF. We extracted DNA from fasting peripheral blood samples and then detected the promoter methylation levels of RUNX2 using quantitative methylation-specific PCR. Relationships between UF concentration, RUNX2 promoter methylation and BMD changes were analyzed using generalized linear model and logistic regression. Results showed in EFG (UF concentration > 1.6 mg/L), BMD was negatively correlated with UF concentration (ß: -0.14; 95%CI: -0.26, -0.01) and RUNX2 promoter methylation (ß: -0.13; 95%CI: -0.22, -0.03) in women. The methylation rate of RUNX2 promoter increased by 2.16% for each 1 mg/L increment in UF concentration of women in EFG (95%CI: 0.37, 3.96). No any significant associations between UF concentration, RUNX2 promoter methylation, and BMD were observed in the individuals in CG. Mediation analysis showed that RUNX2 promoter methylation mediated 18.2% (95% CI: 4.2%, 53.2%) of the association between UF concentration and BMD of women in EFG. In conclusion, excessive fluoride exposure (>1.6 mg/L) is associated with changes of BMD in women, and this association is mediated by RUNX2 promoter methylation.


Assuntos
Densidade Óssea/efeitos dos fármacos , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Exposição Ambiental/análise , Fluoretos/toxicidade , Poluentes Químicos da Água/toxicidade , Absorciometria de Fóton , Adulto , Idoso , Densidade Óssea/genética , China , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Estudos Transversais , Metilação de DNA/efeitos dos fármacos , Feminino , Fluoretos/urina , Humanos , Masculino , Pessoa de Meia-Idade , Regiões Promotoras Genéticas , Inquéritos e Questionários , Poluentes Químicos da Água/urina
17.
Compr Psychiatry ; 103: 152205, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32977245

RESUMO

AIM: This study was aimed to compare the psychological strains between suicides and suicide attempters, so as to verify the hypothesis whether the stronger psychological strains increase the odds of suicide death. METHOD: The suicide data were collected by a psychological autopsy (PA) study, and the suicide attempt data were from the same rural areas in China. Information was obtained with face to face structured questionnaires interviews. A social value conflict scale, measures of aspiration and deprivation, and Moos's Coping Response Inventory (CRI) were used to measure psychological strains. The Spearman correlation and Logistic regression method were applied to analyse and estimate the odds ratio (OR) of fatality of suicide behaviours from psychological strains. RESULTS: The value strain, aspiration strain, deprivation strain, and coping strain were all significantly stronger among the suicides than suicide attempters (P < 0.001). Each psychological strain had statistical correlation with suicide behaviour (P < 0.001). The univariable and multivariable Logistic regressions all indicated that rural residence, being never married, and the psychological strains were the independent lethality factors of suicidal behaviours. CONCLUSIONS: Psychological strains were the independent lethality factors of suicide behaviours. Stronger psychological strains increase the odds of suicide death. The intervention based on the Strain Theory of Suicide could decrease the odds risk of suicide death.

18.
BMC Vet Res ; 16(1): 308, 2020 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-32843036

RESUMO

BACKGROUND: Portal hypertension is a severe complication caused by various chronic liver diseases. The standard methods for detecting portal hypertension (hepatic venous pressure gradient and free portal pressure) are available in only a few hospitals due to their technical difficulty and invasiveness; thus, non-invasive measuring methods are needed. This study aimed to establish and assess a novel model to calculate free portal pressure based on biofluid mechanics. RESULT: Comparison of each dog's virtual and actual free portal pressure showed that a biofluid mechanics-based model could accurately predict free portal pressure (mean difference: -0.220, 95% CI: - 0.738 to 0.298; upper limit of agreement: 2.24, 95% CI: 1.34 to 3.14; lower limit of agreement: -2.68, 95% CI: - 3.58 to - 1.78; intraclass correlation coefficient: 0.98, 95% CI: 0.96 to 0.99; concordance correlation coefficient: 0.97, 95% CI: 0.93 to 0.99) and had a high AUC (0.984, 95% CI: 0.834 to 1.000), sensitivity (92.3, 95% CI: 64.0 to 99.8), specificity (91.7, 95% CI: 61.5 to 99.8), positive likelihood ratio (11.1, 95% CI: 1.7 to 72.8), and low negative likelihood ratio (0.08, 95% CI: 0.01 to 0.6) for detecting portal hypertension. CONCLUSIONS: Our study suggests that the biofluid mechanics-based model was able to accurately predict free portal pressure and detect portal hypertension in canines. With further research and validation, this model might be applicable for calculating human portal pressure, detecting portal hypertensive patients, and evaluating disease progression and treatment efficacy.

19.
Artigo em Inglês | MEDLINE | ID: mdl-32824886

RESUMO

OBJECTIVES: This study aimed to examine the prevalence of medication non-adherence among older adults with diabetes mellitus (DM) in Shandong province, China and to identify its influencing factors. METHODS: A sample of 1002 older adults aged 60 or above with DM was analyzed. Medication adherence was measured using the Morisky-Green-Levine (MGL) Medication Adherence Scale. Descriptive statistical analysis, chi-square test, univariate and multivariate logistic regression analyses were employed. RESULTS: The prevalence of self-reported medication non-adherence among older adults with DM was 19.9%. Female respondents (adjusted odds ratio (AOR) = 1.56, 95% CI: 1.09-2.24) and respondents who perceived medication adherence to be unimportant (AOR = 1.69, 95% CI: 1.05-2.74) were more likely to experience medication non-adherence. Respondents with 5 years of disease duration or longer were less likely (AOR = 0.63, 95% CI: 0.46-0.87) to experience medication non-adherence. CONCLUSIONS: This study showed that about one out of five older adults with DM in Shandong province, China, experienced medication non-adherence, and that gender, disease duration and perceived importance of medication adherence were associated with medication non-adherence in this population group. Provision of counseling and health education programs could be the future priority to raise patients' awareness of the importance of medication adherence and improve patients' self-management of DM.

20.
Front Physiol ; 11: 807, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32792974

RESUMO

Insects' olfactory receptor plays a central role in detecting chemosensory information from the environment. Odorant receptors (ORs) and ionotropic receptors (IRs) are two types of olfactory receptors, and they are essential for the recognition of ligands at peripheral neurons. Apriona germari (Hope) (Coleoptera: Cerambycidae) is one of the most serious insect pests that cause damage to economic trees and landscaping trees, resulting in massive environmental damages and economic losses. Olfactory-based management strategy has been suggested as a promising strategy to control this wood-boring beetle. However, the olfactory perception mechanism in A. germari is now almost unknown. In the present study, RNA sequencing analysis was used to determine the transcriptomes of adult A. germari antennae. Among 36,834 unigenes derived from the antennal assembly, we identified 42 AgerORs and three AgerIRs. Based on the tissue expression pattern analysis, 27 AgerORs displayed a female-biased expression. Notably, AgerOR3, 5, 13, 33, and 40 showed a significant female-biased expression and were clustered with the pheromone receptors of Megacyllene caryae in the phylogenetic tree, suggesting that these AgerORs could be potential pheromone receptors for sensing male-produced sex pheromones in A. germari. The AgerIRs expression profile demonstrated that AgerIR2 had high expression levels in male labial palps, suggesting that this receptor may function to detect female-deposited trail-sex pheromone blend of A. germari. In addition, the phylogenetic tree showed that the Orco gene of five cerambycidae species was highly conservative. These results provide a foundation for further studies on the molecular mechanisms of olfactory chemoreception in A. germari apart from suggesting novel targets for the control of this pest in the future.

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