Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Sci Adv ; 8(10): eabn1778, 2022 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-35275710

RESUMO

Schrödinger's cat originates from the famous thought experiment querying the counterintuitive quantum superposition of macroscopic objects. As a natural extension, several "cats" (quasi-classical objects) can be prepared into coherent quantum superposition states, which is known as multipartite cat states demonstrating quantum entanglement among macroscopically distinct objects. Here, we present a highly scalable approach to deterministically create flying multipartite Schrödinger's cat states by reflecting coherent-state photons from a microwave cavity containing a superconducting qubit. We perform full quantum state tomography on the cat states with up to four photonic modes and confirm the existence of quantum entanglement among them. We also witness the hybrid entanglement between discrete-variable states (the qubit) and continuous-variable states (the flying multipartite cat) through a joint quantum state tomography. Our work provides an enabling step for implementing a series of quantum metrology and quantum information processing protocols based on cat states.

2.
Front Mol Biosci ; 8: 751770, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34631800

RESUMO

Background: Primary aldosteronism is caused by aldosterone overproduction. While conventional hematoxylin-eosin staining can demonstrate morphological abnormality, it cannot provide any functional histopathological information. We aimed to identify the diagnostic, functional and prognostic value of CYP11B2, CYP11B1, and ß-catenin immunostaining in unilateral hyperaldosteronism. Method: A total of 134 patients with unilateral hyperaldosteronism were recruited in our study. The expression of CYP11B2, CYP11B1, and ß-catenin was evaluated semiquantitatively on 134 patients' sections using immunohistochemistry technology and the relationship with clinical data was assessed. Results: Patients were classified into four subtypes based on CYP11B2 staining as below: (1)118 patients with unilateral single aldosterone-producing adenoma (APA), (2)11 with unilateral multiple APA, (3)four with aldosterone-producing cell cluster (APCC), and (4)one with an undefined source. Adjusted CYP11B2 H-score was correlated with serum aldosterone, aldosterone to renin ratio (ARR), and serum potassium. In the abnormal ß-catenin staining group, hypertension duration, aldosterone, ARR, cortisol, tumor diameter, tumor area, and CYP11B2 H-score were significantly higher than those of the wild-type group. Serum potassium level was significantly lower in the abnormal ß-catenin staining group. Age, gender, BMI, family history of hypertension, adjusted CYP11B2 and CYP11B1 H-scores differed significantly between complete clinical success and incomplete clinical success groups. Age, gender and family history of hypertension were independently associated with complete clinical success based on multivariate logistic regression analysis. Conclusion: CYP11B2 immunostaining could improve the differential diagnosis of unilateral hyperaldosteronism. Adjusted CYP11B2 H-score could be used as a histopathological marker to reflect the severity of unilateral APA. Dysregulation of Wnt/ß-catenin signaling and impaired ß-catenin degradation may provoke the proliferation and enhance the steroidogenic ability of APA tumor cells, indicating that the Wnt pathway might be a potential, actionable, therapeutic target in the treatment of hyperaldosteronism. Age, sex and family history of hypertension were independent predictors of clinical outcome after adrenalectomy for unilateral hyperaldosteronism.

3.
Int J Biol Macromol ; 175: 396-405, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33545182

RESUMO

To remove the bisphenol AF (BPAF) from aqueous solution, two different types of lignin-based aromatic porous polymers (LAPP-1 and LAPP-2) were fabricated via one-pot crosslinking of lignin with 1,4-dichloroxylene and 4,4'-bis(chloromethyl)-1,1'-biphenyl, respectively. The successful synthesis of LAPPs was confirmed by FTIR and XPS, SEM, TEM and N2 adsorption-desorption analysis. Then, batch adsorption experiments were conducted to investigate adsorption properties toward BPAF. Based on the results, the adsorption processes were in accordance with the pseudo-second-order kinetic model and the Freundlich isotherm model, and the thermodynamic studies showed that the adsorption was a spontaneous and exothermic process. It is remarkable that LAPPs exhibited good adsorption performance in wide ranges of pH and ionic strength as well as in recycling process. Notably, compared to LAPP-1, LAPP-2 exhibited higher adsorption capacity for BPAF, which can be ascribed to its higher porosity and content of aromatic ring. Moreover, the comprehensive analysis of experimental and theoretical results indicated that the π-π interactions and pore adsorption may jointly drive the uptake process of BPAF. Considering the simple fabrication method employed and excellent BPAF adsorption performance, LAPPs provided new insights into the development of advanced lignin-based adsorbents for removal of BPAF from water.


Assuntos
Compostos Benzidrílicos/isolamento & purificação , Lignina/síntese química , Fenóis/isolamento & purificação , Purificação da Água/métodos , Adsorção , Compostos Benzidrílicos/química , Concentração de Íons de Hidrogênio , Cinética , Fenóis/química , Polímeros/síntese química , Porosidade , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Termodinâmica , Água/química , Poluentes Químicos da Água/química
4.
Cancer Manag Res ; 12: 3203-3210, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32440217

RESUMO

OBJECTIVE: Differences in body mass index (BMI) were used to analyze the survival and prognosis of SCCHN patients. PATIENTS AND METHODS: A retrospective cohort study was conducted to select 323 patients who underwent surgical treatment for SCCHN from June 2013 to June 2016. The patients were divided into a healthy BMI group (BMI<24kg/m2), an overweight group (24kg/m2≤BMI<28kg/m2) and an obese group (BMI≥28 kg/m2). Various statistical methods were used to summarize and analyze clinical data, complications, disease specific survival (DSS), the overall survival (OS), and recurrence-free survival (RFS) within the last 3 y. RESULTS: At 3 y, OS (54.40%) and DSS (51.94%) were slightly lower in the obese group compared with the overweight (64.62%, 61.92%) and healthy BMI groups (64.66%, 65.02%), but no statistical significance was found in DSS (P=0.178), OS (P=0.123) and RFS (P=0.362). The difference in operation duration (P=0.008) and bleeding volume (P=0.001) in obese patients was consistent with those in diabetes mellitus (P=0.002) and coronary heart disease (P=0.000). A high incidence of pharyngeal fistula was observed in obese (P=0.014) and overweight patients (P=0.025), but mouth floor fistula (P=0.038), lung infection (P=0.047), fat liquefaction (P=0.003) and lower extremities deep venous thrombosis (P=0.020) were only found in the obese group. Cox univariatable and multivariatable analysis showed that clinical stage, T stage, and N stage were independent prognostic factors for patients with SCCHN, which was not related to BMI. CONCLUSION: BMI was associated with a higher probability of complications. However, BMI had no significant correlation with 3-year OS, RFS and DSS, and was not a prognostic indicator for patients with SCCHN.

5.
ACS Appl Mater Interfaces ; 11(47): 43996-44006, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31682099

RESUMO

Liver cancer is a leading cause of cancer morbidity and mortality worldwide, especially in China. Sorafenib (SRF) is currently the most commonly used systemic agent against advanced hepatocellular carcinoma (HCC), which is the most common type of liver cancer. However, HCC patients have only limited benefit and suffer a serious side effect from SRF. Therefore, new approaches are urgently needed to improve the therapeutic effectiveness of SRF and reduce its side effect. In our current study, we developed a self-imaging and self-delivered nanodrug with SRF and indocyanine (ICG) to improve the therapeutic effect of sorafenib against HCC. With the π-π stacking effect between SRF and ICG, a one-step nanoprecipitation method was designed to obtain the SRF/ICG nanoparticles (SINP) via self-assembly. Pluronic F127 was used to shield the SINP to further improve the stability in an aqueous environment. The stability, photothermal effect, cell uptake, ROS production, cytotoxicity, tumor imaging, and tumor-targeting and tumor-killing efficacy of the SINP were evaluated in vitro and in vivo by using an HCC cell line Huh7 and its xenograft tumor model. We found that our designed SINP showed monodisperse stability and efficient photothermal effect both in vitro and in vivo. SINP could rapidly enter Huh7 cells and achieve potent cytotoxicity under near-infrared (NIR) laser irradiation partly by producing a great amount of reactive oxygen species (ROS). SINP had significantly improved stability and blood half-life, and could specifically target tumor via the enhanced permeability and retention (EPR) effect in vivo. In addition, SINP showed improved cytotoxicity in both subcutaneous and orthotopic HCC implantation models in vivo. Overall, this rationally designed sorafenib delivery system with a very high loading capacity (33%) has considerably improved antitumor efficiency in vitro and could completely eliminate subcutaneous tumors without any regrowth in vivo. In conclusion, our self-imaging and self-delivered nanodrug could improve the efficacy of SRF and might be a potential therapy for HCC patients.


Assuntos
Antineoplásicos/administração & dosagem , Apoptose/efeitos dos fármacos , Hipertermia Induzida , Verde de Indocianina/administração & dosagem , Neoplasias/tratamento farmacológico , Espécies Reativas de Oxigênio/metabolismo , Sorafenibe/administração & dosagem , Animais , Antineoplásicos/química , Linhagem Celular Tumoral , Terapia Combinada , Sinergismo Farmacológico , Feminino , Humanos , Verde de Indocianina/química , Camundongos Endogâmicos BALB C , Camundongos Nus , Neoplasias/metabolismo , Neoplasias/fisiopatologia , Neoplasias/terapia , Sorafenibe/química
6.
Phys Rev Lett ; 123(8): 080501, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31491216

RESUMO

Searching topological states in artificial systems has recently become a rapidly growing field of research. Meanwhile, significant experimental progress on observing topological phenomena has been made in superconducting circuits. However, topological insulator states have not yet been reported in this system. Here, for the first time, we experimentally realize a tunable dimerized spin chain model and observe the topological magnon insulator states in a superconducting qubit chain. Via parametric modulations of the qubit frequencies, we show that the qubit chain can be flexibly tuned into topologically trivial or nontrivial magnon insulator states. Based on monitoring the quantum dynamics of a single-qubit excitation in the chain, we not only measure the topological winding numbers, but also observe the topological magnon edge and defect states. Our experiment exhibits the great potential of tunable superconducting qubit chain as a versatile platform for exploring noninteracting and interacting symmetry-protected topological states.

7.
Sci Adv ; 5(1): eaav2761, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30746476

RESUMO

Generative adversarial learning is one of the most exciting recent breakthroughs in machine learning. It has shown splendid performance in a variety of challenging tasks such as image and video generation. More recently, a quantum version of generative adversarial learning has been theoretically proposed and shown to have the potential of exhibiting an exponential advantage over its classical counterpart. Here, we report the first proof-of-principle experimental demonstration of quantum generative adversarial learning in a superconducting quantum circuit. We demonstrate that, after several rounds of adversarial learning, a quantum-state generator can be trained to replicate the statistics of the quantum data output from a quantum channel simulator, with a high fidelity (98.8% on average) so that the discriminator cannot distinguish between the true and the generated data. Our results pave the way for experimentally exploring the intriguing long-sought-after quantum advantages in machine learning tasks with noisy intermediate-scale quantum devices.

8.
Curr Med Res Opin ; 35(6): 1091-1096, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30550344

RESUMO

OBJECTIVE: To compare the efficacy and safety of a twice daily injection of insulin aspart (BIAsp) 30 and BIAsp50 in patients with type 2 diabetes mellitus (T2DM) poorly controlled with oral hypoglycemic agents (OHAs). METHODS: In this 12 week prospective, randomized, parallel trial, a total of 80 T2DM patients, 59 ± 10 years old with a disease duration of 9.3 ± 6.6 years and HbA1c >7% despite large doses of metformin and sulfonylurea administration, were randomized to receive BIAsp30 (n = 40) or BIAsp50 (n = 40). The primary endpoint was a change in HbA1c at week 12. RESULTS: The changes in HbA1c from baseline were -2.5% ± 1.0% in the BIAsp50 group and -2.5% ± 1.2% in the BIAsp30 group (p = .897). No difference was observed in the rate of HbA1c target achievement (<7.0%) between BIAsp50 (42.5%) and BIAsp30 (32.5%) (p = .495). The change in fasting plasma glucose (FPG) in the BIAsp50 group was lower than that in the BIAsp30 group (p < .001), while the change in two-hour postprandial blood glucose (2hPBG) was higher and blood glucose excursion was lower in the BIAsp50 group than that in the BIAsp30 group (p < .001, p < .001). A significant improvement in HbA1c was observed with BIAsp50 in subgroups with baseline blood glucose excursion >7.8 mmol/L or 2hPBG >17.6 mmol/L compared with BIAsp30. There were no differences in hypoglycemia or body weight between groups. CONCLUSIONS: Compared with BIAsp30, BIAsp50 showed greater efficacy in patients with baseline BG excursion >7.8 mmol/L or 2hPBG >17.6 mmol/L as well as good safety for hypoglycemia. CLINICAL TRIAL REGISTRATION: ChiCTR-IIR-16008958.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/administração & dosagem , Insulina Aspart/administração & dosagem , Idoso , Glicemia/análise , Diabetes Mellitus Tipo 2/sangue , Esquema de Medicação , Feminino , Hemoglobina A Glicada/análise , Humanos , Injeções , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
9.
Sci Adv ; 3(5): e1603159, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28508079

RESUMO

Wave-particle complementarity lies at the heart of quantum mechanics. To illustrate this mysterious feature, Wheeler proposed the delayed-choice experiment, where a quantum system manifests the wave- or particle-like attribute, depending on the experimental arrangement, which is made after the system has entered the interferometer. In recent quantum delayed-choice experiments, these two complementary behaviors were simultaneously observed with a quantum interferometer in a superposition of being closed and open. We suggest and implement a conceptually different quantum delayed-choice experiment by introducing a which-path detector (WPD) that can simultaneously record and neglect the system's path information, but where the interferometer itself is classical. Our experiment is realized with a superconducting circuit, where a cavity acts as the WPD for an interfering qubit. Using this setup, we implement the first twofold delayed-choice experiment, which demonstrates that the system's behavior depends not only on the measuring device's configuration that can be chosen even after the system has been detected but also on whether we a posteriori erase or mark the which-path information, the latter of which cannot be revealed by previous quantum delayed-choice experiments. Our results represent the first demonstration of both counterintuitive features with the same experimental setup, significantly extending the concept of quantum delayed-choice experiment.

10.
ACS Appl Mater Interfaces ; 9(11): 9426-9436, 2017 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-28247750

RESUMO

Combination chemotherapy has been proposed to achieve synergistic effect and minimize drug dose for cancer treatment in clinic application. In this article, the stimuli-responsive polymeric nanogels (<100 nm in size) based on poly(acrylic acid) were designed as codelivery system for doxorubicin and cisplatin to overcome drug resistance. By chelation, electrostatic interaction, and π-π stacking interactions, the nanogels could encapsulate doxorubicin and cisplatin with designed ratio and high capacity. Compared with free drugs, the nanogels could deliver more drugs into MCF-7/ADR cells. Significant accumulation in tumor tissues was observed in the biodistribution experiments. The in vitro antitumor studies demonstrated the superior cell-killing activity of the nanogel drug delivery system with a combination index of 0.84, which indicated the great synergistic effect. All the antitumor experimental data revealed that the combination therapy was effective for the multidrug-resistant MCF-7/ADR tumor with reduced side effects.


Assuntos
Nanopartículas , Cisplatino , Doxorrubicina , Sistemas de Liberação de Medicamentos , Resistencia a Medicamentos Antineoplásicos , Quimioterapia Combinada , Humanos , Células MCF-7
11.
ACS Biomater Sci Eng ; 3(3): 381-391, 2017 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-33465935

RESUMO

The rational design of particle-based cancer theranostic agents, combining diagnostic and therapeutic features in a single entity, has emerged as an effective approach toward personalized cancer therapy; however, creating a flexible assembly of specific targeting ligands with regard to a broad range of tumor tissues and cells is still challenging. Here, we present a convenient and highly variable on-site assembly strategy for the preparation of multifunctional doxorubicin (DOX)-loaded nanocargos with magnetic supraparticles (MSPs) as a core and redox-degradable poly(methylacrylic acid-co-N,N-bis(acryloyl) cystamine) (P(MAA-co-Cy) as the shell, which could be simultaneously modified with multiple targeting ligands through parallelized bioconjugation on the basis of a streptavidin-biotin (SA-BT) interaction. Under physiological conditions similar to those of the cytoplasm of tumor cells, DOX could be released in a controlled manner from these nanocargos to specific tumor sites, while dual-ligand modified nanocargos showed remarkable proliferation inhibition for the HeLa cells and the SK-OV-3 cells that overexpressed both folate as well as integrin receptors. The experimental results demonstrated that the on-site assembly strategy described herein opens access to highly efficient targeting drug delivery systems toward personalized cancer targeting therapy by incorporating functional diversity, which can be easily achieved through highly efficient and parallelized one-step bioconjugation.

12.
Nano Lett ; 16(10): 6245-6251, 2016 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-27632023

RESUMO

Graphene quantum dots could be an ideal host for spin qubits and thus have been extensively investigated based on graphene nanoribbons and etched nanostructures; however, edge and substrate-induced disorders severely limit device functionality. Here, we report the confinement of quantum dots in few-layer graphene with tunable barriers, defined by local strain and electrostatic gating. Transport measurements unambiguously reveal that confinement barriers are formed by inducing a band gap via the electrostatic gating together with local strain induced constriction. Numerical simulations according to the local top-gate geometry confirm the band gap opening by a perpendicular electric field. We investigate the magnetic field dependence of the energy-level spectra in these graphene quantum dots. Experimental results reveal a complex evolution of Coulomb oscillations with the magnetic field, featuring kinks at level crossings. The simulation of energy spectrum shows that the kink features and the magnetic field dependence are consistent with experimental observations, implying the hybridized nature of energy-level spectrum of these graphene quantum dots.

13.
Phys Rev Lett ; 117(1): 010502, 2016 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-27419550

RESUMO

Coherently manipulating multipartite quantum correlations leads to remarkable advantages in quantum information processing. A fundamental question is whether such quantum advantages persist only by exploiting multipartite correlations, such as entanglement. Recently, Dale, Jennings, and Rudolph negated the question by showing that a randomness processing, quantum Bernoulli factory, using quantum coherence, is strictly more powerful than the one with classical mechanics. In this Letter, focusing on the same scenario, we propose a theoretical protocol that is classically impossible but can be implemented solely using quantum coherence without entanglement. We demonstrate the protocol by exploiting the high-fidelity quantum state preparation and measurement with a superconducting qubit in the circuit quantum electrodynamics architecture and a nearly quantum-limited parametric amplifier. Our experiment shows the advantage of using quantum coherence of a single qubit for information processing even when multipartite correlation is not present.

14.
J Control Release ; 225: 96-108, 2016 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-26812007

RESUMO

Biodegradable materials used for drug delivery are of great demand due to their ability to degrade into low molecular weight species and further excrete from the body by metabolism. Herein, we report a new kind of zinc(II) crosslinked poly(methacrylic acid) nanohydrogels (ZCLNs) inspired by zinc finger proteins with dual stimuli-triggered degradation (glutathione and pH) for the first time. Compared with the disulfide bond crosslinked nanohydrogels, this new kind of ZCLNs is beneficial to the degradation of a wide range of cells, including normal cells. Ex vivo fluorescence images showed that the DOX-loaded folate-PEG conjugated zinc(II)-crosslinked polymeric nanohydrogels (FPZCLNs-15) preferentially accumulated in tumor tissue and the accumulation in normal tissues was much less compared with DOX-loaded PZCLNs-15 (non-targeted nanohydrogels) and free DOX, the FPZCLNs-15 (targeting system) delivered DOX to the tumor site with approximately 3.6- and 1.6-fold higher than free DOX and PZCLNs-15, respectively. Meanwhile, the PZCLNs-15 and FPZCLNs-15 reduced the concentration of DOX in the heart by 3.2- and 5.0-fold respectively, as compared to the free DOX. Moreover, a superior tumor growth inhibition and negligible damage to normal organs like the heart and kidney, which is reported to be vulnerable to DOX-associated side effects, are further demonstrated.


Assuntos
Antineoplásicos/administração & dosagem , Doxorrubicina/administração & dosagem , Ácido Fólico/análogos & derivados , Hidrogéis/administração & dosagem , Polietilenoglicóis/administração & dosagem , Ácidos Polimetacrílicos/administração & dosagem , Zinco/administração & dosagem , Animais , Antineoplásicos/química , Antineoplásicos/farmacocinética , Antineoplásicos/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/química , Doxorrubicina/farmacocinética , Doxorrubicina/uso terapêutico , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Feminino , Ácido Fólico/administração & dosagem , Ácido Fólico/química , Ácido Fólico/farmacocinética , Ácido Fólico/uso terapêutico , Glutationa/metabolismo , Células HEK293 , Células HeLa , Humanos , Hidrogéis/química , Hidrogéis/farmacocinética , Hidrogéis/uso terapêutico , Concentração de Íons de Hidrogênio , Camundongos Endogâmicos BALB C , Nanoestruturas/administração & dosagem , Nanoestruturas/química , Nanoestruturas/uso terapêutico , Neoplasias/tratamento farmacológico , Neoplasias/metabolismo , Neoplasias/patologia , Polietilenoglicóis/química , Polietilenoglicóis/farmacocinética , Polietilenoglicóis/uso terapêutico , Ácidos Polimetacrílicos/química , Ácidos Polimetacrílicos/farmacocinética , Ácidos Polimetacrílicos/uso terapêutico , Carga Tumoral/efeitos dos fármacos , Zinco/química , Zinco/farmacocinética , Zinco/uso terapêutico , Dedos de Zinco
15.
Nat Commun ; 6: 8970, 2015 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-26611724

RESUMO

The Schrodinger's cat thought experiment highlights the counterintuitive concept of entanglement in macroscopically distinguishable systems. The hallmark of entanglement is the detection of strong correlations between systems, most starkly demonstrated by the violation of a Bell inequality. No violation of a Bell inequality has been observed for a system entangled with a superposition of coherent states, known as a cat state. Here we use the Clauser-Horne-Shimony-Holt formulation of a Bell test to characterize entanglement between an artificial atom and a cat state, or a Bell-cat. Using superconducting circuits with high-fidelity measurements and real-time feedback, we detect correlations that surpass the classical maximum of the Bell inequality. We investigate the influence of decoherence with states up to 16 photons in size and characterize the system by introducing joint Wigner tomography. Such techniques demonstrate that information stored in superpositions of coherent states can be extracted efficiently, a crucial requirement for quantum computing with resonators.

16.
Zhongguo Zhong Yao Za Zhi ; 40(16): 3314-8, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26790313

RESUMO

In recent years, the incidence of tic disorders has increased, and it is not uncommon for the patients to treat the disease. The pathogenesis and pathogenesis of Western medicine are not yet clear, the clinical commonly used western medicine has many adverse reactions, traditional Chinese medicine (TCM) research is increasingly valued. Based on the software of TCM inheritance assistant system, this paper discusses Ding Yuanqing's experience in treating tic disorder with Professor. Collect yuan Qing Ding professor in treating tic disorder of medical records by association rules Apriori algorithm, complex system entropy clustering without supervision and data mining method, carries on the analysis to the selected 800 prescriptions, to determine the frequency of use of prescription drugs, the association rules between the drug and digging out the 12 core combination and the first six new prescription, medication transferred to the liver and extinguish wind, cooling blood and relieving convulsion, Qingxin soothe the nerves, with the card cut, flexible application, strict compatibility.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Transtornos de Tique/tratamento farmacológico , Mineração de Dados , Bases de Dados Bibliográficas , Prescrições de Medicamentos , Quimioterapia Combinada , Medicamentos de Ervas Chinesas/química , Humanos , Resultado do Tratamento
17.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 48(6): 330-3, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24119999

RESUMO

OBJECTIVE: To examine the expression of LIM mineralization protein-1 (LMP-1) in the apical papilla and dental pulp tissues of human immature permanent teeth and to investigate the role of LMP-1 in the development and maturation of pulp-dentin complex. METHODS: Forty-eight healthy premolars in need of extraction for orthodontic treatment were obtained with 24 immature permanent teeth and 24 mature permanent teeth. After extraction, the apical papilla was detached from the dental pulp in the immature permanent tooth and the dental pulp of mature permanent tooth was rapidly removed. The samples were divided into 3 groups: group 1, apical papilla of immature permanent teeth (root formed 2/3 of its full length); group 2, dental pulp tissues of immature permanent teeth; group 3, dental pulp tissues of mature permanent teeth. There were 24 samples for each group.Half of them were used for reverse transcriptien-PCR (RT-PCR) detection, and the other half were used for Western blotting detection. Band intensities were quantified using Meta Morph software 6.2.6 and subsequently normalized by dividing the band gray value of the target gene by the intensity of its corresponding ß-actin. Two-sample t test was used to analyze the difference of expression intensity between group 1 and group 2 as well as group 2 and group 3 with SPSS 13.0 software package.Statistical significance was established as P < 0.05. RESULTS: As indicated by RT-PCR, LMP-1 expressed in the apical papilla, dental pulp of immature permanent teeth and the dental pulp of mature permanent teeth were 0.25 ± 0.09, 0.46 ± 0.24 and 0.31 ± 0.10 respectively. The expression intensity of LMP-1 mRNA in the dental pulp tissues of human immature permanent teeth were significantly higher than that in the apical papilla tissues(t = 2.92) and that in the dental pulp tissues of human mature permanent teeth (t = 2.31) (P < 0.05). As indicated by Western blotting, LMP-1 expressed in the three groups were 0.33 ± 0.08, 0.82 ± 0.10 and 0.52 ± 0.19 respectively. The expression intensity of LMP-1 protein in the dental pulp tissues of human immature permanent teeth were significantly higher than that in the apical papilla tissues(t = 3.33) and that in the dental pulp tissues of human mature permanent teeth (t = 3.11) (P < 0.05). CONCLUSIONS: LMP-1 were positively expressed in all the samples including apical papilla of immature permanent teeth, dental pulp of immature and mature permanent teeth at the level of both mRNA and protein, but with different intensity. LMP-1 could play an important role in the development and maturation of pulp-dentin complex.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteínas do Citoesqueleto/metabolismo , Papila Dentária/metabolismo , Polpa Dentária/metabolismo , Proteínas com Domínio LIM/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Adolescente , Adulto , Western Blotting , Criança , Proteínas do Citoesqueleto/genética , Dentição Permanente , Humanos , Proteínas com Domínio LIM/genética , Desenvolvimento Maxilofacial , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...