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1.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4670-4676, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872663

RESUMO

This research was performed to establish the HPLC fingerprint of Sabia parviflora. HPLC method was carried out on a Thermo Accucore-C18(4. 6 mm×150 mm,2. 6 µm) column by 30% tetrahydrofuran in methyl alcohol-acetonitrile-0. 1% phosphate solution as mobile phase at a flow rate of 1. 0 m L·min-1,the column temperature was 30 ℃ and the detection wavelength was 360 nm. The fingerprints were further evaluated by chemometrics methods including similarity analysis,hierarchical clustering analysis,and principal component analysis. In HPLC fingerprint,15 common peaks were selected as the common peaks,and 6 contents of them were identified. The similarity degrees of 38 batches of the samples was more than 0. 710,and the samples were divided into 6 clusters by their quality difference. The method was precision,repeatable,stable,simple and reliable,which could be used for quality control and evaluation of S. parviflora.


Assuntos
Medicamentos de Ervas Chinesas , Cromatografia Líquida de Alta Pressão , Análise por Conglomerados , Análise de Componente Principal , Controle de Qualidade
2.
J Thorac Dis ; 11(10): 4188-4196, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31737302

RESUMO

Background: Prone position ventilation (PPV) is an important strategy for patients with severe acute respiratory distress syndrome (ARDS). This prospective study investigated the use of electromyography of the diaphragm (EMGdi) for monitoring respiratory drive in patients with moderate to severe ARDS during long-term PPV. Methods: An integrated nostril-gastric feeding tube containing an esophageal electrode and balloon was placed in 14 patients with severe ARDS prior to PPV. EMGdi and trans-pulmonary pressure (∆PL) data were collected before PPV (baseline), every 2 h during PPV, and 2 h after the restoration of supine position ventilation (post-2 h SPV). Results: In ARDS patients, the static compliance of the chest wall was significantly decreased after PPV. EMGdi levels were slightly lower in the early, middle, and late stages of PPV compared with baseline. Patients who received neuromuscular blocker experienced a greater drop in EMGdi from baseline than those who did not. Conclusions: For ARDS patients, EMGdi was slightly decreased after prolonged PPV. This is contrary to the change in diaphragm electromyography during normal body position changes. Monitoring EMGdi regularly during PPV in ARDS patients is feasible and can be used as a reference for lung protective ventilation strategies.

3.
Zhongguo Zhong Yao Za Zhi ; 44(2): 265-269, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30989943

RESUMO

This study is based on the data analysis of medicinal plant resources and diversity collected from the fourth Chinese traditional medicine resource survey( pilot). Through the analysis of relevant data from 33 census pioneer plots in Guizhou province( area),a total of 265 families,1 432 genera and 5 296 species of medicinal resources were reported,including algae,fungi,lichens,mosses,a total of 43 genera and 35 families,57,48 families,120 genera and 453 species of ferns,gymnosperms 11 families,22 genera and 61 species,167 families,1 243 genera and 4 721 species of angiosperms,4 genera and 4 families four medicinal animals.Compared with the data related to the third survey of traditional Chinese medicine resources,the number of ferns,gymnosperms and angiosperms in the fourth survey has increased far more than that of the third survey. From the regional distribution of medicinal resources,the composition of the genus,the type of life,and the location of the medicine,the richness of the medicinal plant resources in Guizhou province is not only reflected in many types,but also in the variety of medicinal resources. These studies provide a scientific basis for vigorously developing the Chinese herbal medicine industry and the sustainably using medicinal plant resources in Guizhou province.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Plantas Medicinais/classificação , China , Cycadopsida , Gleiquênias , Magnoliopsida
4.
Zhonghua Nan Ke Xue ; 24(2): 109-115, 2018 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30156068

RESUMO

Objective: To elucidate the possible role of human lysozyme-like protein 4 (LYZL4) in fertilization and characterize its enzymatic properties. METHODS: The localization of LYZL4 in human spermatozoa was investigated by immunofluorescence staining, the sources of LYZL4 on the sperm surface examined by RT-PCR, and the role of LYZL4 in fertilization assessed by the zona-free hamster egg penetration test. The recombinant plasmid pPIC9K-LYZL4 was constructed and its expression induced with methanol after transformed into competent Pichia pastoris GS115. The recombinant LYZL4 protein (rLYZL4) was purified from the fermentation supernatant and subsequently identified by Western blot. The hyaluronan binding ability of rLYZL4 was determined by ELISA and the muramidase activity, hyaluronidase activity, and free radical scavenging ability examined by spectrophotometric methods. RESULTS: Immunodetection with a specific antiserum localized LYZL4 on the acrosomal membrane of mature spermatozoa, which was exclusively secreted from the testis and epididymis as shown by RT-PCR. Immunoneutralization of LYZL4 significantly decreased the number of human spermatozoa bound to zona-free hamster eggs in a dose-dependent manner in vitro. The recombinant protein was expressed successfully by the P. pastoris strain GS115. Purified rLYZL4 exhibited a potent hyaluronan binding ability and a strong free radical scavenging ability but no muramidase or hyaluronidase activity. CONCLUSIONS: LYZL4 secreted from the testis and epididymis is localized on the acrosomal membrane of mature spermatozoa and plays a role in sperm-egg binding as well as in binding hyaluronan and scavenging free radicals, which suggests that it might be a multi-functional molecule contributive to sperm protection and sperm-egg binding.


Assuntos
Acrossomo/enzimologia , Muramidase/fisiologia , Interações Espermatozoide-Óvulo/fisiologia , Animais , Western Blotting , Cricetinae , Ensaio de Imunoadsorção Enzimática , Epididimo , Feminino , Fertilização/fisiologia , Depuradores de Radicais Livres/metabolismo , Humanos , Ácido Hialurônico/metabolismo , Masculino , Muramidase/análise , Pichia , Plasmídeos/metabolismo , Proteínas Recombinantes/análise , Proteínas Recombinantes/metabolismo , Espermatozoides/enzimologia , Testículo
5.
Zhong Yao Cai ; 36(10): 1594-8, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24761667

RESUMO

OBJECTIVE: To study the feasibility and significance of the method to identify medicinal plants through the observation and statistics of 9 species of Sabia medical plants from Guizhou province. METHODS: Leaf epidermis characteristics were observed, measured by optical microscope and analyzed by SPSS 17.0 software. RESULTS: All of these plants had some differences in indumentum, cell morphology and size, and had significant difference in the length and circumference of lower epidermis cells. CONCLUSION: The method combining microscopic observation with statistics can be used as the classification and identification basis of medicinal plants and materials of Sabia genus.


Assuntos
Magnoliopsida/classificação , Magnoliopsida/ultraestrutura , Epiderme Vegetal/ultraestrutura , Folhas de Planta/ultraestrutura , Plantas Medicinais/ultraestrutura , China , Magnoliopsida/anatomia & histologia , Magnoliopsida/citologia , Microscopia Eletrônica de Varredura , Epiderme Vegetal/citologia , Folhas de Planta/anatomia & histologia , Folhas de Planta/citologia , Plantas Medicinais/anatomia & histologia , Plantas Medicinais/citologia , Especificidade da Espécie
6.
Mol Biol Rep ; 39(5): 5529-35, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22189539

RESUMO

Activated rheumatoid arthritis (RA) fibroblast-like synoviocytes (RAFLSs) play a central role in both initiating and driving RA. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been documented to induce apoptosis only in a small proportion of RAFLSs, which is followed by an induction of proliferation in surviving cells. Apigenin, a chemopreventive bioflavonoid, exhibits proapoptotic activity in many types of cells. In the present study, we sought to determine whether apigenin could enhance the cytotoxic effect of TRAIL on activated RAFLSs. Human RAFLSs isolated from patients with RA were treated with TRAIL (1 nM), apigenin (20 µM), or their combination, and subjected to apoptosis analysis after a 24-h incubation and proliferation analysis after a 72-h incubation. Apoptosis assay revealed that TRAIL or apigenin alone induced a marked apoptosis in RAFLS and their combination yielded a synergistic increase in RAFLS apoptosis. Immunoblotting analysis of apoptosis regulators demonstrated that combined treatment with apigenin increased caspase-3 expression and activity and decreased the Bcl-2/Bax ratio relative to treatment with TRAIL alone. The presence of apigenin significantly restrained TRAIL-induced RAFLS proliferation, coupled with restoration of the expression of two cell-cycle inhibitors p21 and p27. Moreover, the combination with apigenin blunted TRAIL-induced activation of the phosphatidylinositol 3-kinase (PI3-K)/Akt pathway. Our data collectively demonstrate that apigenin sensitizes RAFLS to TRAIL-induced apoptosis and counteracts TRAIL-dependent RAFLS proliferation, which is likely mediated through inactivation of PI3-K/Akt signaling pathway.


Assuntos
Apigenina/farmacologia , Apoptose/efeitos dos fármacos , Artrite Reumatoide/patologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Líquido Sinovial/citologia , Ligante Indutor de Apoptose Relacionado a TNF/farmacologia , Proliferação de Células/efeitos dos fármacos , Fibroblastos/enzimologia , Humanos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Líquido Sinovial/efeitos dos fármacos
7.
J Clin Lab Anal ; 25(5): 344-9, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21919069

RESUMO

The detection of Mycobacterium tuberculosis-specific antibodies in human sera has been a rapid and important diagnostic aid for tuberculosis (TB) control and prevention. However, any single antigen is not enough to be used to cover the antibody profiles of all TB patients. In this study, a novel fusion protein was constructed using gene splicing by overlap extension (SOEing), and then the antibody level against it in 171 TB patients and 86 controls was evaluated by enzyme-linked immunosorbent assay. Compared with the three individual antigen (16 kDa: sensitivity 19.9%, specificity 96.5%; MPT64: sensitivity 75.4%, specificity 34.9%; 38 kDa: sensitivity 33.3%, specificity 83.7%), the fusion protein antigen (sensitivity 42.1%, specificity 89.5%) gave the best diagnostic performance with the largest receiver operating characteristic curve area 0.656 (95% confidence interval [CI], 0.590-0.721; P<0.01). These results suggested that the novel fusion protein antigen successfully constructed by gene SOEing provided the improved diagnostic performance for TB, and other mycobacterial multiepitope fusion proteins may also be worthy of investigation for further enhancing the detection sensitivity.


Assuntos
Antígenos de Bactérias/imunologia , Mycobacterium tuberculosis/imunologia , Proteínas Recombinantes de Fusão/imunologia , Testes Sorológicos/métodos , Tuberculose/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos de Bactérias/química , Antígenos de Bactérias/genética , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Epitopos/análise , Humanos , Pessoa de Meia-Idade , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/isolamento & purificação , Reação em Cadeia da Polimerase , Curva ROC , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Sensibilidade e Especificidade , Tuberculose/sangue
8.
J Bacteriol ; 193(16): 4300-1, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21685274

RESUMO

Mycobacteriosis is on the increase. Nontuberculous mycobacteria (NTM) are resistant to most antituberculosis drugs naturally. We determined the complete genome sequence of a novel NTM strain, JDM601, of the Mycobacterium terrae complex, which was isolated from a patient with tuberculosis-like disease and with various antibiotic resistances.


Assuntos
Genoma Bacteriano , Mycobacterium/classificação , Mycobacterium/genética , Humanos , Dados de Sequência Molecular , Infecções por Micobactéria não Tuberculosa/microbiologia , Especificidade da Espécie
9.
Exp Biol Med (Maywood) ; 236(7): 859-66, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21676922

RESUMO

Activated rheumatoid arthritis fibroblast-like synoviocytes (RAFLSs) play an important role in the initiation and progression of rheumatoid arthritis (RA). Rapid proliferation and defective apoptosis of RAFLSs are two main mechanisms contributing to synovial hyperplasia. Berberine, the major constituent of Coptidis Rhizoma, has been widely used as an antitumor and anti-inflammation agent. Here we show that berberine significantly inhibited cell proliferation of serum-starved human RAFLSs in a dose-dependent manner. Cell cycle analysis of berberine-treated RAFLSs indicated a cell cycle arrest at the G0/G1 phase. The inhibitory effects of berberine correlated with an induction of cyclin-dependent kinase (CDK) inhibitors Cip1/p21 and Kip1/p27 and a reduction of CDK2, CDK4 and CDK6, and cyclins D1, D2 and E. Furthermore, an apoptosis assay showed that berberine treatment increased apoptotic death of RAFLSs, which was associated with an increased expression of proapoptotic protein Bax and decreased expression of antiapoptotic proteins Bcl-2 and Bcl-xl, disruption of mitochondrial membrane potential, and activation of caspase-3, caspase-9 and poly (ADP-ribose) polymerase. Taken together, these results demonstrate that berberine exerts antiproliferative effects against RAFLSs, likely through deregulation of numerous cell cycle and apoptosis regulators, thus having potential therapeutic implications in the treatment of RA.


Assuntos
Anti-Inflamatórios/farmacologia , Berberina/farmacologia , Fibroblastos/efeitos dos fármacos , Líquido Sinovial/citologia , Líquido Sinovial/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Artrite Reumatoide/patologia , Ciclo Celular/efeitos dos fármacos , Proteínas de Ciclo Celular/metabolismo , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Humanos
10.
Zhonghua Fu Chan Ke Za Zhi ; 45(4): 292-7, 2010 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-20646543

RESUMO

OBJECTIVE: To investigate the expression of exogenous gene transferred by piggyBac (PB) transposon in various gynecological malignant cell lines and reveal its potential application of gene therapy in gynecological cancer. METHODS: Amplified herpes simplex virus thymidine kinase (HSV-tk) gene coding region by PCR and integrated it into PB expression vector, PB[Act-RFP]DS, for reconstructing PB[Act-RFP, HSV-tk]DS (pPB/TK). By using different transfection reagents: FuGENE HD, jetPEI, lipofectamine 2000, pPB/TK together with helper plasmid Act-PBase were cotransfected into four mostly common gynecological malignant tumor cell lines HeLa, JEG-3, SKOV3 and HEC-1B. The mRFP1 report gene expressions was observed and detected by fluorescence microscope and flow cytometry to analyze transfection efficiency. The expressions of HSV-tk and mRFP1 gene were detected by reverse transcription PCR (RT-PCR). The cytotoxic effect of various concentration of pro-drug ganciclovir (GCV) for transfected cells was detected by methyl thiazole tetrazolium assay. The transfected cells were positive sorted by flow cytometry and limiting diluted to obtain the stable transfected cell line. The insertion sites of foreign gene transferred by PB transposon in genome were analyzed by inverse PCR. RESULTS: (1) Double digests analysis and sequences test demonstrated that pPB/TK vector was reconstructed successfully. (2) Using three different transfective reagents, PB transposon transferred HSV-tk gene and mRFP1 gene into HeLa, HEC-1B, SKOV3 and JEG-3 cell efficiently, and the transfection efficiency of pPB/TK for the same cell was different by using different transfective reagents; in Hela cell, the transfection efficiency of FuGENE HD [(78.7+/-9.2)%] was higher than that of lipofectamine 2000 [(54.1+/-11.4)%] and jetPEI [(46.5+/-7.4)%, all P<0.05]; using the same transfective reagent, the transfection efficiency of pPB/TK was also different on various cell lines, using FuGENE HD, the transfection efficiency of pPB/TK on HeLa, JEG-3 and SKOV3 cell was (78.7+/-9.2)%, (74.4+/-8.9)% and (83.2+/-9.7)% respectively, which all were higher than that on HEC-1B [(39.5+/-8.7)%, P<0.05]. (3) RT-PCR showed that there were the mRNA expression of HSV-tk and mRFP1 in all cell lines. (4) 50% inhibitory concentration of GCV for transfected cells, HeLa, JEG-3, SKOV3 and HEC-1B, was 1.29, 3.35, 0.09 and 13.28 microg/ml respectively. Inhibitory effect of GCV (10 microg/ml) on SKOV3 transfected with pPB/TK was (86+/-9)%, which was superior to that transfected with pORF-HSVtk alone [(52+/-12)%, P<0.05]. (5) The insertion sites of PB transposon in the target cells genome were located at TTAA sites. mRFP1 expression still could be detected in three months after transfected. CONCLUSIONS: PB transposon could transfer exogenous gene into various gynecological malignant cells, which could integrated into genome and obtain a long-term and stable expression. It is expected that PB transposon may supply a more efficient and safer transgene technology platform for gene therapy in gynecological cancer.


Assuntos
Elementos de DNA Transponíveis/genética , Técnicas de Transferência de Genes , Vetores Genéticos , Proteínas Luminescentes/genética , Timidina Quinase/genética , Sobrevivência Celular , Feminino , Citometria de Fluxo , Ganciclovir/administração & dosagem , Ganciclovir/farmacologia , Expressão Gênica , Terapia Genética , Neoplasias dos Genitais Femininos/genética , Neoplasias dos Genitais Femininos/metabolismo , Neoplasias dos Genitais Femininos/terapia , Células HeLa , Humanos , Proteínas Luminescentes/metabolismo , Plasmídeos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Simplexvirus/genética , Simplexvirus/metabolismo , Timidina Quinase/metabolismo , Transfecção , Transgenes
11.
Tuberculosis (Edinb) ; 89(4): 278-84, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19559650

RESUMO

In this study, we describe the development and evaluation of a novel multiple-antigen ELISA for rapid diagnosis and screening of active tuberculosis (TB). The humoral immune responses of 136 active TB patients and 57 healthy subjects against antigens Rv3425, 38kDa and lipoarabinomannan (LAM) from Mycobacterium tuberculosis H37Rv were examined by ELISA. Three essential results were obtained. (i) Rv3425 antigen is a potential candidate for serodiagnosis of active TB. Of 136 active TB patients, Rv3425 antigen provided a sensitivity of 31.6%, lower than that of LAM antigen, but higher than that of 38kDa antigen, with an overall specificity of 100%. (ii) For 62 smear-negative pulmonary TB patients and 15 extra-pulmonary TB patients, the multiple-antigen test provided a sensitivity of 43.5% and 26.7%, respectively, representing an improvement over acid-fast bacilli (AFB) smear-based diagnosis. (iii) Compared with the single-antigen ELISA and the two available commercial kits, the multiple-antigen test offered the highest accuracy (71.0%). In conclusion, the multiple-antigen ELSIA test based on Rv3425, 38kDa, and LAM antigens is a potentially useful tool for the serodiagnosis and screening of active TB. Combinations of Rv3425 with other mycobacterial antigens may also be worthy of further investigation.


Assuntos
Antígenos de Bactérias/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Testes Sorológicos/métodos , Tuberculose/diagnóstico , Adolescente , Adulto , Idoso , Antígenos de Bactérias/genética , Feminino , Genótipo , Humanos , Imunoglobulina G/sangue , Lipopolissacarídeos/imunologia , Masculino , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética , RNA Ribossômico 23S/genética , Kit de Reagentes para Diagnóstico , Sensibilidade e Especificidade , Tuberculose/imunologia , Tuberculose Pulmonar/diagnóstico , Tuberculose Pulmonar/imunologia , Adulto Jovem
12.
Zhongguo Zhong Yao Za Zhi ; 33(5): 594-7, 2008 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-18536388

RESUMO

To introduce the advance on the species, ecological environment, distribution areas, the number of the species and efficacy of geographic distribution new records of medicinal plants in Guizhou. This article provides a basis for the collection and conservation as well as reasonable development of the genetic resources of medicinal plants.


Assuntos
Plantas Medicinais/química , Plantas Medicinais/crescimento & desenvolvimento , China , Medicamentos de Ervas Chinesas/farmacologia , Geografia
13.
Public Health ; 121(4): 258-65, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17307207

RESUMO

OBJECTIVES: To understand the association between the outbreak of severe acute respiratory syndrome (SARS) and meteorological factors and air pollution. STUDY DESIGN: An ecological study was conducted. METHODS: Three hundred and fifty primary probable SARS cases diagnosed in mainland China between 1 January and 31 May 2003, and their 6727 close contacts during the period of their clinical symptoms before admission, were included in this study. Of the 6727 close contacts, 135 (2.0%) later developed clinical symptoms and were diagnosed as probable SARS cases. The daily meteorological data and daily air pollution data during the same SARS outbreak period in mainland China were used in the data analysis. Logistic regression analyses were conducted to explore the association between the secondary attack rate of SARS and meteorological factors and air pollution. RESULTS: In univariate analyses, daily average temperature (DAT), daily average air pressure (DAAP), and daily average relative humidity (DARH) were inversely associated with secondary attack rate (P<0.001); a significant positive association was found for daily hours of sunshine (DHS) (P<0.001). In multivariate analyses, factors associated with secondary attack rate were DAAP (odds ratio (OR)=0.53, 95% confidence interval (CI): 0.42, 0.66), DARH (OR=0.73, 95% CI: 0.53, 1.00), and daily average wind velocity (DAWV; OR=0.81, 95% CI: 0.68, 0.96). Adjustment for the onset time of a primary case led to little change in the results. In addition, in Hebei Province, a major affected area in China, only DAWV (OR=0.38, 95% CI: 0.20, 0.72) was a significant predictor of secondary attack rate with adjustment for the onset time of primary case. In Inner Mongolia, another major affected area in China, DAWV (OR=0.50, 95% CI: 0.26, 0.94) and DHS (OR=0.27, 95% CI: 0.09, 0.81) were significant predictors of secondary attack rate with adjustment for the onset time of primary case. CONCLUSIONS: Our results suggest that the SARS outbreak was significantly associated with DAWV, and that DAAP, DARH and DHS may also have influenced the SARS outbreak to some extent. However, because of ecological fallacy and uncontrolled confounding effects that may have biased the results, the association between the SARS outbreak and these meteorological factors and air pollution deserve further investigation.


Assuntos
Poluição do Ar/efeitos adversos , Surtos de Doenças , Síndrome Respiratória Aguda Grave/epidemiologia , Tempo (Meteorologia) , China/epidemiologia , Humanos , Modelos Logísticos
14.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 18(5): 275-7, 2006 May.
Artigo em Chinês | MEDLINE | ID: mdl-16700990

RESUMO

OBJECTIVE: To explore the effects of mild hypothermia on expression of N-methyl-D-aspartate receptor-1 (NMDAR1) in hippocampus neurons after cardiopulmonary resuscitation (CPR) in rats. METHODS: Twenty-four male SD rats were randomly divided into normal control group, normal temperature group, and mild hypothermia group, with 8 rats in each group. The cerebral edema model after CPR was replicated by the sealed bottle method in rats in both normal temperature group and mild hypothermia group. The rats in the mild hypothermia group were treated with mild hypothermia after the model was established. The change in expression of NMDAR1 in hippocampus neurons in rat was determined with semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and pathologic changes in brain tissue were observed in both groups. RESULTS: The cerebral edema was ameliorated, NMDAR1 mRNA and protein in cerebral hippocampus neurons were significantly lower in hypothermia group than control group with significant difference (NMDAR1 mRNA: 80.48+/-0.03 vs. 80.64+/-0.18, P<0.05 ). CONCLUSION: Mild hypothermia can down regulate the expression of NMDAR1 mRNA and protein level, lower positive ion concentration and thus decrease cerebral edema, so mild hypothermia can serve as a treatment measure for cerebral edema after CPR.


Assuntos
Edema Encefálico/terapia , Reanimação Cardiopulmonar , Hipotermia Induzida , Neurônios/metabolismo , Receptores de N-Metil-D-Aspartato/biossíntese , Animais , Edema Encefálico/metabolismo , Hipocampo/citologia , Masculino , RNA Mensageiro/genética , Ratos , Ratos Sprague-Dawley , Receptores de N-Metil-D-Aspartato/genética
15.
Am J Epidemiol ; 163(3): 211-6, 2006 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-16339050

RESUMO

Many epidemiologists have agreed that a refined estimate of the incubation period of severe acute respiratory syndrome (SARS) would need a sample size of about 200 cases and appropriate statistical methods enabling the inclusion of cases with defined periods of exposure. However, no such studies have been reported so far. Besides, determinants of the SARS incubation period remain unclear. In this study, 209 probable SARS cases with documented episodes of exposure between March 1 and May 31, 2003, in mainland China were included. A nonparametric method was used to analyze these data with defined periods of exposure to obtain the refined estimate of the SARS incubation period. Furthermore, the authors also explored the influence of various factors on the SARS incubation period by analysis of variance, linear regression analysis, and analysis of covariance. The estimates of mean and variance of the SARS incubation period were 5.29 days and 12.33 days(2), respectively; 90% of patients would have an incubation period of less than 11.58 days with a probability of 0.8, and 99% of patients would have an incubation of less than 22.22 days with a probability of 0.9. The affected area showed a highly significant effect on the incubation period (p < 0.001), but the contact pattern, occupation, gender, and age did not.


Assuntos
Busca de Comunicante , Síndrome Respiratória Aguda Grave/transmissão , Análise de Variância , China/epidemiologia , Intervalos de Confiança , Bases de Dados como Assunto , Progressão da Doença , Feminino , Humanos , Masculino , Estudos Retrospectivos , Fatores de Risco , Síndrome Respiratória Aguda Grave/diagnóstico , Síndrome Respiratória Aguda Grave/epidemiologia , Fatores de Tempo
16.
Prenat Diagn ; 25(7): 577-81, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16032768

RESUMO

OBJECTIVES: To provide a new, reliable noninvasive method for fetal sex determination. METHODS: Fetal sex was detected in 32 early pregnant women by identifying the amelogenin gene in maternal plasma using nested PCR analysis. First, the 122/128 bp of X-Y homologous region containing 6 bp deletions in the intron 3 of amelogenin gene in X chromosome was amplified, and then the nested PCR was carried out, whose 3' end of the upstream primer is just located in the deletion region. The fetus was male or female, depending on whether it had the 89-bp nested PCR product or not. RESULTS: The 89 bp of nested PCR product was detected in 19 plasma samples obtained from pregnant women, deducing they bear the male fetus and the remaining pregnant women bear female. When compared with the birth outcome, two samples were pseudo-positive. The coincidence was 93.8%. This method had high sensitivity that even trace amount of target fetal DNA (10 pg) could be detected. CONCLUSIONS: This conventional nested PCR analysis of amelogenin gene promises to be a reliable method for noninvasive fetal sex determination at early pregnancy using maternal plasma DNA.


Assuntos
Proteínas do Esmalte Dentário/genética , Diagnóstico Pré-Natal , Análise para Determinação do Sexo/métodos , Amelogenina , Grupo com Ancestrais do Continente Asiático , Cromossomos Humanos X/química , Cromossomos Humanos Y/química , DNA/sangue , DNA/química , Primers do DNA , Proteínas do Esmalte Dentário/sangue , Feminino , Idade Gestacional , Humanos , Masculino , Reação em Cadeia da Polimerase/métodos , Valor Preditivo dos Testes , Gravidez , Primeiro Trimestre da Gravidez
17.
Zhonghua Liu Xing Bing Xue Za Zhi ; 26(3): 153-8, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15941495

RESUMO

OBJECTIVE: To build an epidemic model of severe acute respiratory syndrome (SARS) to be used to quantitatively evaluate the effectiveness of intervention measures. METHODS: Following the nature of SARS epidemics, an epidemic model of SARS based on SEIR model of infectious disease was built, in which case management group was set up and some parameters related to control measures were included. As an example, data gathered from Beijing was fitted to illustrate its application to quantitatively evaluate the intervention measures. RESULTS: In the newly developed model of epidemics, parameters related to control measures could easily be modified. When scenario assumption was used, a model on SARS outbreak under various circumstances of intervention measures could be set. Thus, quantitative evaluation of intervention measures could perfectly be caried out. Pilot study revealed that the fitness existed in modeling the outbreak of SARS in Beijing and the intervention measures implemented around April 20, 2003, had major contribution to the control of SARS. CONCLUSION: The model of SARS epidemics seemed to be stable and reliable to be used to evaluate the effectiveness of intervention measures implemented during the SARS outbreak in a quantitative way.


Assuntos
Surtos de Doenças/estatística & dados numéricos , Modelos Biológicos , Síndrome Respiratória Aguda Grave/epidemiologia , China/epidemiologia , Métodos Epidemiológicos , Humanos , Modelos Estatísticos , Síndrome Respiratória Aguda Grave/terapia , Síndrome Respiratória Aguda Grave/transmissão
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