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1.
Nat Commun ; 11(1): 5241, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33067443

RESUMO

To understand how the RuvC catalytic domain of Class 2 Cas proteins cleaves DNA, it will be necessary to elucidate the structures of RuvC-containing Cas complexes in their catalytically competent states. Cas12i2 is a Class 2 type V-I CRISPR-Cas endonuclease that cleaves target dsDNA by an unknown mechanism. Here, we report structures of Cas12i2-crRNA-DNA complexes and a Cas12i2-crRNA complex. We reveal the mechanism of DNA recognition and cleavage by Cas12i2, and activation of the RuvC catalytic pocket induced by a conformational change of the Helical-II domain. The seed region (nucleotides 1-8) is dispensable for RuvC activation, but the duplex of the central spacer (nucleotides 9-15) is required. We captured the catalytic state of Cas12i2, with both metal ions and the ssDNA substrate bound in the RuvC catalytic pocket. Together, our studies provide significant insights into the DNA cleavage mechanism by RuvC-containing Cas proteins.

2.
Aging Clin Exp Res ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33068266

RESUMO

BACKGROUND: Cemented hemiarthroplasty to treat femoral neck fractures (FNFs) in elderly patients is controversial. Therefore, this study aimed to compare cemented vs uncemented outcomes. METHODS: A literature review using Pubmed, EMBASE, Cochrane Library, and Science Citation Index databases was conducted. Studies comparing outcomes of cemented with uncemented hemiarthroplasty for FNFs in elderly patients up to March 2020 were included. Dichotomous outcomes were pooled and reported as relative risk (RR) or odds ratio (ORs), while continuous outcomes were pooled and reported as the mean difference (MD) or standardized mean difference (SMD). RESULTS: The analysis included 39 studies with a total of 112 576 patients. Pooled analysis revealed that compared with cemented, patients with uncemented intervention had better outcomes for intraoperative blood loss (OR 0.19; 95% CI 0.01-0.37), systolic blood pressure (OR 2.83; 95% CI 1.51-5.28), surgery duration (SMD, 0.51; 95% CI 0.2-0.81), length of anesthesia (OR 0.28; 95% CI 0.11-0.45), 6-month mortality (OR 1.11; 95% CI 1.03-1.2), cardiovascular accidents (OR 2.14; 95% CI 1.07-4.28), respiratory failure (OR 8.26; 95% CI 1.38-49.4), fat embolisms (OR 1.58; 95% CI 1.29-1.93), and heterotrophic ossification (OR 2.3; 95% CI 1.3-4.06), but more intraoperative accidents (OR 0.34; 95% CI 0.26-0.45), postoperative fractures (OR 0.27; 95% CI 0.21-0.34), reoperations (OR 0.59; 95% CI 0.53-0.65), and revisions (OR 0.62; 95% CI 0.44-0.88). CONCLUSIONS: Meta-analysis of hemiarthroplasty outcomes shows that elderly patients who underwent uncemented vs cemented procedures had better results for several factors that are important for not only improved recovery in elderly populations, but also more intraoperative and postoperative risks.

3.
Nat Commun ; 11(1): 5129, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046710

RESUMO

Zinc finger E-box binding homeobox 1 (Zeb1) has been demonstrated to participate in the acquisition of the properties of cancer stem cells (CSCs). However, it is largely unknown how signals from the tumor microenvironment (TME) contribute to aberrant Zeb1 expression. Here, we show that Zeb1 depletion suppresses stemness, colonization and the phenotypic plasticity of breast cancer. Moreover, we demonstrate that, with direct cell-cell contact, TME-derived endothelial cells provide the Notch ligand Jagged1 (Jag1) to neighboring breast CSCs, leading to Notch1-dependent upregulation of Zeb1. In turn, ectopic Zeb1 in tumor cells increases VEGFA production and reciprocally induces endothelial Jag1 in a paracrine manner. Depletion of Zeb1 disrupts this positive feedback loop in the tumor perivascular niche, which eventually lessens tumor initiation and progression in vivo and in vitro. In this work, we highlight that targeting the angiocrine Jag1-Notch1-Zeb1-VEGFA loop decreases breast cancer aggressiveness and thus enhances the efficacy of antiangiogenic therapy.

4.
Rapid Commun Mass Spectrom ; : e8969, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33047398

RESUMO

RATIONALE: Alzheimer's disease (AD) is a chronic, severe, progressive neurodegenerative disorder associated with cognitive and memory impairment, and it ultimately causes death. Most approved drugs can only alleviate some of the symptoms of AD, but no interventions have been found that reverse the underlying disease mechanisms. Rhodiola crenulata extract (RCE) has been reported to alleviate AD symptoms in rats. However, its underlying mechanism of action is still unclear. METHODS: A brain lipidomics study was conducted to investigate the protective effects of RCE against AD in rats to identify potential biomarkers of AD, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) coupled with high-performance reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC). Differences in lipid metabolism profiles were evaluated using multivariate statistical analysis. Finally, the possible mechanism of action of RCE on AD was investigated by analysing metabolic pathways. RESULTS: The RPLC/HILIC FT-ICR MS results showed 20 lipid components with significant differences between the control and model groups. After RCE administration, the levels of 10 lipids in AD rats tended to shift toward reference levels. The pathway analysis revealed that the protective effect of RCE against AD might be related to regulation of glycerophospholipid metabolism. CONCLUSIONS: This study provides a novel perspective on the potential intervention mechanism of RCE in the treatment of AD.

5.
Biotechnol Bioeng ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33022744

RESUMO

As a precursor of graphene, graphene oxide (GO) exhibits excellent mechanical, thermal, and electrical properties, besides appreciable biocompatibility in tissue engineering applications. However, the current GO-3D fabrication technology is still in need of optimization and simplification in order to ensure fine architecture and reasonable mechanical property, which would further promote the performance of GO as bio-scaffolds in cell or microorganism attachment and in material transformation. To address this issue, we proposed a GO ink, with appreciable rheological properties and excellent printing performance via high-speed centrifugation and ferric ion-assisted cross-linking. A woodpile structure with controllable micro-pores was produced by micro-extrusion-based 3D printing technology followed by an optimized freeze-drying process. Cellular adhesion and viability were verified by inoculation and culture of HepaRG cells using the fabricated GO 3D structure, thus suggesting ferric ion-assisted cross-linking and controllable pore distribution to improve the performance of GO construct as a bio-scaffold for in-vitro liver tissue models. This article is protected by copyright. All rights reserved.

6.
Theranostics ; 10(24): 11092-11109, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33042272

RESUMO

Rationale: Paclitaxel resistance is a major concern when treating triple-negative breast cancer (TNBC) patients. We aimed to identify candidates causing paclitaxel resistance and explore their significance in TNBC therapeutics. Methods: A genome-wide CRISPR screening, integrated with transcriptome analyses, was performed to identify candidates involved in paclitaxel-resistant TNBCs. Cell proliferation, cytotoxicity, immunofluorescent staining, and xenograft assays were conducted to verify the phenotypes of paclitaxel resistance induced by candidate genes, both in vitro and in vivo. RNA sequencing, Western blotting, and chromatin immunoprecipitation assays were used to explore the underlying mechanisms. Results: MEF2-interacting transcriptional repressor (MITR), the truncated isoform of histone deacetylase 9 (HDAC9) lacking the deacetylation domain, was enriched in paclitaxel-resistant cells. Elevated MITR expression resulted in increased interleukin-11 (IL11) expression and activation of downstream JAK/STAT3 signaling. Mechanistically, MITR counteracted MEF2A-induced transcriptional suppression of IL11, ultimately causing paclitaxel resistance. By contrast, pharmacological inhibition of JAK1/2 by ruxolitinib reversed paclitaxel resistance both in vitro and in vivo. Conclusion: Our in vitro and in vivo genetic and cellular analyses elucidated the pivotal role of MITR/MEF2A/IL11 axis in paclitaxel resistance and provided a novel therapeutic strategy for TNBC patients to overcome poor chemotherapy responses.

7.
J Int Med Res ; 48(10): 300060520959220, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33045881

RESUMO

OBJECTIVE: To investigate the relationship between Helicobacter pylori (H. pylori) infection and gallstones or gallbladder polyps. METHODS: This retrospective analysis included 27,881 individuals who underwent health examinations that included a H. pylori test and an abdominal ultrasound scan. Patients were divided into four groups: gallbladder polyp (P group), gallstone (S group), gallstone and gallbladder polyp (SP group), and no gallbladder disease (N group). Case-control matching was used to select the participants in the control group. RESULTS: The mean ages of participants in the P, S, and SP groups were all significantly higher than the mean age of participants in the N group. The proportions of participants with each type of body mass index significantly differed between the N and P groups, and between the N and S groups. In total 45.7% of participants exhibited H. pylori infection. After case-control matching, the proportion of participants with H. pylori infection did not significantly differ according to the presence or absence of gallbladder polyps. Similar results were observed regarding gallstones, as well as gallstones and gallbladder polyps. CONCLUSION: H. pylori infection might not be related to gallbladder polyps or gallstones.

9.
Biomaterials ; 265: 120416, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-33007612

RESUMO

Hepatocellular carcinoma (HCC) is one of the most lethal tumors worldwide. This study aims to address the lack of faithful and available in vitro models for patient-specific drug screening for HCC. We recently established a novel modeling system using three-dimensional (3D) bioprinting technology and constructed hepatorganoids with HepaRG cells, which retain the liver function and prolong the survival of mice with liver failure after abdominal transplantation. Here we extend this modeling system to establish individualized model for hepatocellular carcinoma. HCC specimens were obtained from six patients after surgery. Primary HCC cells were isolated and mixed with gelatin and sodium alginate to form the bioink for printing. Patient-derived three-dimensional bio-printed HCC (3DP-HCC) models were successfully established afterward and grew well during long-term culture. These models retained the features of parental HCCs, including stable expression of the biomarker, stable maintenances of the genetic alterations and expression profiles. 3DP-HCC models are capable of displaying the results of drug screening intuitively and quantitatively. In conclusion, 3DP-HCC models are faithful in vitro models that are reliable in long-term culture and able to predict patient-specific drugs for personalized treatment.

10.
Environ Monit Assess ; 192(10): 665, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33001326

RESUMO

With economic development and the acceleration of urbanization, China's energy demand has gradually increased and brought a lot of energy-related CO2 emissions. Energy-related CO2 emissions are affected by a variety of factors. Quantifying the correlation between energy-related CO2 and driving factors and constructing the driving factor system are conducive to predict the future energy-related CO2 emissions and analyze the impact of driving factors. In this paper, the improved grey relational analysis (IGRA) was proposed to screen the influencing factors of energy-related CO2 emissions considering the sample difference, and the factor analysis (FA) was used to reduce dimensionality of the influencing factors. Then, a carbon dioxide emission forecasting model based on the bacterial foraging optimization algorithm (BFO) and the least square support vector machine (LSSVM) was proposed. Empirical analysis results of Hebei show that the LSSVM optimized BFO significantly improves the accuracy of energy-related CO2 emissions forecasting, and IGRA-FA-BFOLSSVM model is significantly better than BP, PSOBP, SVM, and LSSVM models. The mean absolute percentage error (MAPE) of the proposed model is 0.374%. The forecasting results of the supplementary case show that the model has better generalization ability. In addition, education and technological progress have proven to be important drivers of energy-related CO2 emissions. Simultaneously, the research results can also offer more breakthrough points for policy makers to control carbon emissions.


Assuntos
Dióxido de Carbono/análise , Monitoramento Ambiental , China , Desenvolvimento Econômico , Análise Fatorial
11.
Chem Commun (Camb) ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33020765

RESUMO

Metal-catalyzed reductive amination of formyl-containing linkers with N,N-dialkylformamide solvents is concomitant with the solvothermal coordination assembly, leading to novel MOFs functionalized with tertiary aliphatic amino groups. This illustrates a novel one-pot strategy to functionalize MOFs through in situ organic transformation. The UiO-66 MOFs partially functionalized with the amino groups are highly active heterogeneous catalysts for Knoevenagel condensation.

12.
Cancer Commun (Lond) ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33025763

RESUMO

BACKGROUND: Whether non-sentinel lymph node (SLN)-positive melanoma patients can benefit from completion lymph node dissection (CLND) is still unclear. The current study was performed to identify the prognostic role of non-SLN status in SLN-positive melanoma and to investigate the predictive factors of non-SLN metastasis in acral and cutaneous melanoma patients. METHODS: The records of 328 SLN-positive melanoma patients who underwent radical surgery at four cancer centers from September 2009 to August 2017 were reviewed. Clinicopathological data including age, gender, Clark level, Breslow index, ulceration, the number of positive SLNs, non-SLN status, and adjuvant therapy were included for survival analyses. Patients were followed up until death or June 30, 2019. Multivariable logistic regression modeling was performed to identify factors associated with non-SLN positivity. Log-rank analysis and Cox regression analysis were used to identify the prognostic factors for disease-free survival (DFS) and overall survival (OS). RESULTS: Among all enrolled patients, 220 (67.1%) had acral melanoma and 108 (32.9%) had cutaneous melanoma. The 5-year DFS and OS rate of the entire cohort was 31.5% and 54.1%, respectively. More than 1 positive SLNs were found in 123 (37.5%) patients. Positive non-SLNs were found in 99 (30.2%) patients. Patients with positive non-SLNs had significantly worse DFS and OS (log-rank P < 0.001). Non-SLN status (P = 0.003), number of positive SLNs (P = 0.016), and adjuvant therapy (P = 0.025) were independent prognostic factors for DFS, while non-SLN status (P = 0.002), the Breslow index (P = 0.027), Clark level (P = 0.006), ulceration (P = 0.004), number of positive SLNs (P = 0.001), and adjuvant therapy (P = 0.007) were independent prognostic factors for OS. The Breslow index (P = 0.020), Clark level (P = 0.012), and number of positive SLNs (P = 0.031) were independently related to positive non-SLNs and could be used to develop more personalized surgical strategy. CONCLUSIONS: Non-SLN-positive melanoma patients had worse DFS and OS even after immediate CLND than those with non-SLN-negative melanoma. The Breslow index, Clark level, and number of positive SLNs were independent predictive factors for non-SLN status.

13.
Med Oncol ; 37(11): 97, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33001278

RESUMO

Dipeptidyl peptidase-IV (DPP-IV) inhibitors are used to control blood glucose levels in patients with type 2 diabetes. However, the influence of DPP-IV inhibitors on malignant tumors remains unknown. The present study aimed to investigate the effect of the DPP-IV inhibitor saxagliptin on thyroid carcinoma cells. Transwell assays and a nude mouse lung metastasis model were used to evaluate the invasion and metastasis of thyroid carcinoma cells. Western blotting was used to determine the protein levels of migration and invasion-related molecules. We tested the expression and distribution of nuclear factor, erythroid 2 like 2 (NRF2) in thyroid carcinoma cells with and without saxagliptin. Furthermore, we silenced NRF2 and observed saxagliptin's effect on migration and invasion. Quantitative real-time reverse transcription PCR (qRT-PCR) and western blotting were then used to measure the expression of NFR2's downstream molecules (heme oxygenase 1 (HO1), matrix metalloproteinase 2 (MMP2), and vascular endothelial growth factor (VEGF)). A luciferase reporter assay was used to validate whether NRF2 could regulate the transcriptional activity of the HO1 promoter. Saxagliptin enhanced the migratory and invasive ability of thyroid carcinoma cells. MMP2 and VEGF levels were also elevated by saxagliptin treatment. We found that saxagliptin treatment increases the nuclear and cytoplasmic accumulation NRF2. Silencing NRF2 abolished the effect of saxagliptin on migration and invasion. Accordingly, NRF2 silencing downregulated HO1, MMP2, and VEGF levels. The luciferase assay showed that NRF2 activated transcription from the HO1 promoter. Saxagliptin could promote this transcriptional activity by upregulating NRF2. Saxagliptin enhanced the migratory and invasive ability of human thyroid carcinoma cells, as well as the expression of MMP2 and VEGF, by activating the NRF2/HO1 pathway.

14.
IEEE Trans Cybern ; PP2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33027022

RESUMO

The phenomenon of increasing accidents caused by reduced vigilance does exist. In the future, the high accuracy of vigilance estimation will play a significant role in public transportation safety. We propose a multimodal regression network that consists of multichannel deep autoencoders with subnetwork neurons (MCDAEsn). After we define two thresholds of ``0.35'' and ``0.70'' from the percentage of eye closure, the output values are in the continuous range of 0-0.35, 0.36-0.70, and 0.71-1 representing the awake state, the tired state, and the drowsy state, respectively. To verify the efficiency of our strategy, we first applied the proposed approach to a single modality. Then, for the multimodality, since the complementary information between forehead electrooculography and electroencephalography features, we found the performance of the proposed approach using features fusion significantly improved, demonstrating the effectiveness and efficiency of our method.

15.
J Hazard Mater ; 404(Pt A): 124035, 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33035907

RESUMO

Efficient removal of Hg2+ from aqueous solution is key for environmental protection and human health. Herein, a novel composite of nano humboldtine decorated almandine was synthesized from almandine for the removal of Hg2+. Results showed that the Hg2+ removal process followed pseudo-second-order kinetic model and Langmuir equation, and the maximum adsorption capacity was 575.17 mg/g. Furthermore, Hg2+ removal by the composite was pH-dependent and low pH value facilitated the removal of Hg2+. SEM and HADDF-STEM results suggested a new rod morphology was generated and the adsorbed mercury was mainly enriched into this structure after reaction with Hg2+ solution. The removal mechanisms of Hg2+ by the composite was pH dependent, and included ion exchange, surface complexation, reduction and oxidation. Our results demonstrated that the composite was an ideal material for Hg2+ removal and the transformation ways of mercury related species could be a significant but currently underestimated pathway in natural and engineered systems.

16.
J Formos Med Assoc ; 2020 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-32919876

RESUMO

BACKGROUND/PURPOSE: Malignant hyperthermia (MH) is a life-threatening pharmacogenetic disease with only two known causative genes, RYR1 and CACNA1S. Both are huge genes containing numerous exons, and they reportedly only account for 50-70% of known MH patients. Next-generation sequencing (NGS) technology and bioinformatics could help delineate the genetic diagnosis of MH and several MH-like clinical presentations. METHODS: We established a capture-based targeted NGS sequencing framework to examine the whole genomic regions of RYR1, CACNA1S and the 16.6 Kb mitochondrial genome, as well as 12 other genes related to excitation-contraction coupling and/or skeletal muscle calcium homeostasis. We applied bioinformatics analyses to the variants identified in this study and also to the 48 documented RYR1 pathogenic variants. RESULTS: The causative variants were identified in seven of the eight (87.5%) MH families, but in none of the 10 individuals classified as either normal controls (N = 2) or patients displaying MH-like clinical features later found to be caused by other etiologies (N = 8). We showed that RYR1 c.1565A>G (p.Tyr522Cys)(rs118192162) could be a genetic hot spot in the Taiwanese population. Bioinformatics analyses demonstrated low population frequencies and predicted damaging effects from all known pathogenic RYR1 variants. We estimated that more than one in 1149 individuals worldwide carry MH pathogenic variants at RYR1. CONCLUSION: NGS and bioinformatics are sensitive and specific tools to examine RYR1 and CACNA1S for the genetic diagnosis of MH. Pathogenic variants in RYR1 can be found in the majority of MH patients in Taiwan.

17.
Chemistry ; 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32969553

RESUMO

Developing efficient catalysts for the conversion of CO 2 into fuels and value-added chemicals is of great significance to relieve the growing energy crisis and global warming. With the assistance of DFT calculations, it is interesting to find that, different from Al 12 X (X = Be, Al, and C), the alkali-metal-like superatom Al 12 P prefers to combine with CO 2 via a bidentate double oxygen coordination, yielding a stable Al 12 P( η 2 -O 2 C) complex containing an activated radical anion of CO 2 ( i.e. , CO 2 •- ). Thereby, this compound can not only participate in the subsequent cycloaddition reaction with propylene oxide, but also initiate the radical reaction with hydrogen gas to form high value chemicals, revealing that Al 12 P can play an important role in catalyzing these conversion reactions. Considering that Al 12 P has been produced in laboratory, and is capable of absorbing visible light to drive the activation and transformation of CO 2 , we optimistically anticipated that this work could guide the experimentalists to discover such an additional superatom catalyst for CO 2 transformation, and open up a new research field of superatom catalysis.

18.
Medicine (Baltimore) ; 99(36): e21785, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32899005

RESUMO

BACKGROUND: Concurrent cisplatin with radiotherapy (CRT) or concurrent cetuximab with radiotherapy (BRT) improves outcomes in locally advanced head and neck squamous cell carcinoma (HNSCC) compared with radiotherapy alone. Nevertheless, a detailed comparison between CRT and BRT in locally advanced HNSCC is required due to inconclusive results. METHODS: A comprehensive literature search was conducted on PubMed, Web of Science, Cochrane databases, and EMBASE. Studies that evaluated CRT vs BRT in locally advanced HNSCC were included. The primary outcome that was overall survival (OS), whereas the secondary outcomes were progression-free survival (PFS), locoregional control (LRC), and distant metastasis-free survival (DMFS). Pooled hazard ratios (HRs) and the corresponding 95% confidence intervals (CIs) were used to evaluate prognosis. All the analyses were performed using Stata Statistical Software 12.0. RESULTS: Twenty-three studies, with a total of 8701 patients, were considered eligible and included in this meta-analysis. Our results revealed that patients treated with CRT had longer OS (HR = 0.51, 95%CI, 0.41-0.64, P < .001), PFS (HR = 0.37, 95%CI, 0.23-0.60, P < .001), LRC (HR = 0.46, 95%CI, 0.37-0.57, P < .001), and DMFS (HR = 0.56, 95%CI, 0.40-0.77, P < .001) than those treated with BRT. Furthermore, the results of the subgroup analyses were consistent with the primary analysis. CONCLUSIONS: CRT has a better OS, PFS, LRC, and DMFS than BRT in locally advanced HNSCC, and should be the preferred treatment for patients with the disease.


Assuntos
Cetuximab/uso terapêutico , Cisplatino/uso terapêutico , Neoplasias de Cabeça e Pescoço/terapia , Carcinoma de Células Escamosas de Cabeça e Pescoço/terapia , Idoso , Quimiorradioterapia/métodos , Intervalo Livre de Doença , Feminino , Neoplasias de Cabeça e Pescoço/mortalidade , Humanos , Masculino , Pessoa de Meia-Idade , Intervalo Livre de Progressão , Carcinoma de Células Escamosas de Cabeça e Pescoço/mortalidade
19.
Complement Ther Clin Pract ; 41: 101240, 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32977216

RESUMO

BACKGROUND AND PURPOSE: and purpose: Massage has gained increasing attention for reducing peri-operative anxiety. We aimed to investigate the effectiveness of massage for peri-operative anxiety in adults. METHODS: Six English electronic databases were comprehensively searched from their inception to February 2020. Subgroup analysis, quality assessment, sensitivity analysis, meta-regression and publication bias assessment were performed. RESULTS: Twenty-five controlled trials comprising 2494 participants were included. The meta-analysis indicated that massage could significantly reduce peri-operative anxiety for most types of surgical patients. Specifically, it was effective for pre-, intra- and post-operative anxiety. Acupoint or specific body reflex area massage showed a larger effect than general massage did. Massage delivered by professionals and non-professionals were both effective. Massage lasting 10-20 min per session was the most worthy of recommendation. Massage was concomitant with the improvement of peri-operative vital signs and post-operative pain. CONCLUSION: Massage is a promising complementary therapy for ameliorating peri-operative anxiety in adults.

20.
Med Sci Monit ; 26: e927167, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32963215

RESUMO

BACKGROUND Early and rapid identification of severe coronavirus disease 2019 (COVID-19) cases is important. The present study aimed to investigate the predictors of disease severity and thus determine the trends for disease progression early. MATERIAL AND METHODS Patients with COVID-19 were recruited from Fuyang Second People's Hospital from January to February 2020. Patients' demographic, epidemiological, and clinical data were collected, and the relationships between these variables and disease severity were analyzed. RESULTS A total of 158 cases were included according to COVID-19 diagnosis, and the treatment schemes were analyzed for identification of early indicators affecting COVID-19 progression. Severe cases accounted for 18.99% of the diagnosed cases. Analysis showed that patients' age (χ²=10.640,=0.041); the time interval between onset and diagnosis (χ²=7.278, P=0.026); the source of cases (χ²=5.557, P=0.018); fever (χ²=5.676, P=0.014); dyspnea (χ²=113.085, P<0.001); muscle or joint pain (χ²=3.900, P=0.048); chest pain (χ²=13.446, P=0.006); the levels of lymphocytes (t=2.917, P=0.014), C-reactive protein (U=730.00, P<0.001), and aspartate aminotransferase (U=1235.00, P=0.002); damage in both lungs within 3 days of admission (χ²=7.632, P=0.003); and diabetes (χ²=6.675, P=0.010) were significantly correlated with the trend of intensification. CONCLUSIONS Older age, a long time interval from onset to diagnosis, imported cases from an affected area, dyspnea, muscle or joint pain, chest pain during the course of the disease, reduced lymphocytes, elevated C-reactive protein, computed tomography scan showing damage to both lungs within 3 days of admission, and diabetes mellitus are predictors for severe COVID-19.


Assuntos
Betacoronavirus , Infecções por Coronavirus , Pandemias , Pneumonia Viral , Adolescente , Adulto , Fatores Etários , Proteína C-Reativa/análise , Estudos de Casos e Controles , Comorbidade , Infecções por Coronavirus/epidemiologia , Diagnóstico Tardio , Progressão da Doença , Feminino , Humanos , Pulmão/patologia , Masculino , Pessoa de Meia-Idade , Pneumonia Viral/epidemiologia , Prognóstico , Fatores de Risco , Fatores Socioeconômicos , Avaliação de Sintomas , Adulto Jovem
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