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Gastroenterol Rep (Oxf) ; 7(3): 193-198, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31217983


Background: In addition to the stepwise manner of lymph-node metastasis from the primary tumour, the skip lymph-node metastasis (SLNM) was identified as a low-incidence metastasis of gastric cancer (GC). So far, both the mechanism and outcome of SLNM have not been elucidated completely. The purpose of this study was to analyse the clinical significance and the potential mechanism of SLNM in GC patients who had lymph-node metastasis. Methods: Clinicopathological data and follow-up information of 505 GC patients who had lymph-node metastasis were analysed to demonstrate the significance of SLNM in evaluating the prognostic outcome. According to the pathological results, all GC patients who had lymph-node metastasis were categorized into three groups: patients with the perigastric lymph-node metastasis, patients with the perigastric and extragastric lymph-node metastasis and patients with SLNM.Results: Among the 505 GC patients who had lymph-node metastasis, 24 (4.8%) had pathologically identified SLNM. The location of lymph-node metastasis was not significantly associated with 5-year survival rate and overall survival (OS) (P = 0.194). The stratified survival analysis results showed that the status of SLNM was significantly associated with the OS in patients with pN1 GC (P = 0.001). The median OS was significantly shorter in 19 pN1 GC patients with SLNM than in 100 patients with perigastric lymph-node metastasis (P < 0.001). The case-control matched logistic regression analysis results showed that tumour size (P = 0.002) was the only clinicopathological factor that may predict SLNM in pN1 GC patients undergoing curative surgery. Among the 19 pN1 GC patients with SLNM, 17 (89.5%) had metastatic lymph nodes along the common hepatic artery, around the celiac artery or in the hepatoduodenal ligament. Conclusions: SLNM may be considered a potentially practicable indicator for prognosis among various subgroups of pN1 GC patients.

Biometals ; 29(2): 225-34, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26786763


The coccolithophore Emiliania huxleyi is covered with elaborated calcite plates, the so-called coccoliths, which are produced inside the cells. We investigated the incorporation of zinc into the coccoliths of E. huxleyi by applying different zinc and calcium amounts via the culture media and subsequently analyzing the zinc content in the cells and the Zn/Ca ratio of the coccoliths. To investigate the Zn/Ca ratio of coccoliths built in the manipulated media, the algae have first to be decalcified, i.e. coccolith free. We used a newly developed decalcification method to obtain 'naked' cells for cultivation. E. huxleyi proliferated and produced new coccoliths in all media with manipulated Zn/Ca ratios. The cells and the newly built coccoliths were investigated regarding their zinc content and their Zn/Ca ratio, respectively. High zinc amounts were taken up by the algae. The Zn/Ca ratio of the coccoliths was positively correlated to the Zn/Ca ratio of the applied media. The unique feature of the coccoliths was maintained also at high Zn/Ca ratios. We suggest the following pathway of the zinc ions into the coccoliths: first, the zinc ions are bound to the cell surface, followed by their transportation into the cytoplasm. Obviously, the zinc ions are removed afterwards into the coccolith vesicle, where the zinc is incorporated into the calcite coccoliths which are then extruded. The incorporation of toxic zinc ions into the coccoliths possibly due to a new function of the coccoliths as detoxification sites is discussed.

Carbonato de Cálcio/metabolismo , Haptófitas/metabolismo , Microalgas/metabolismo , Poluentes Químicos da Água/metabolismo , Zinco/metabolismo , Células Cultivadas , Haptófitas/efeitos dos fármacos , Haptófitas/ultraestrutura , Microalgas/efeitos dos fármacos , Microalgas/ultraestrutura , Poluentes Químicos da Água/farmacologia , Zinco/farmacologia
Support Care Cancer ; 24(3): 1019-24, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26245496


PURPOSE: Our aim is to test the validity, reliability, and acceptability of the Chinese version of European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Bone Metastases 22 (EORTC QLQ-BM22) module to assess health-related quality of life (HRQOL) in patients with bone metastases in China. METHODS: Patients with histological confirmation of malignancy and bone metastases from Tianjin Cancer Institution and Hospital from June 2013 to April 2014 were enrolled in this study. All patients self-administered the EORTC QLQ-BM22 and the EORTC QLQ-C30. The Karnofsky Performance Scale (KPS) was performed to evaluate scores. The reliability and validity tests of the questionnaires were based on Cronbach's α coefficients, Pearson correlation test, and Wilcoxon rank sum nonparametric test. RESULTS: Internal consistency reliabilities of all the four scales were acceptable. Scales measuring similar HRQOL aspects were found to correlate with one another between EORTC QLQ-BM22 and EORTC QLQ-C30, but differences still existed. Significant differences were demonstrated in the scores of all four subscales of the QLQ-BM22 between the two KPS subgroups (KPS ≤ 80; KPS > 80). Meanwhile, the compliance for item completion of the QLQ-BM22 was satisfactory. CONCLUSIONS: The Chinese version of EORTC QLQ-BM22 is a reliable and valid instrument, which is appropriate for measuring the HRQOL of patients with bone metastases in China.

Conservadores da Densidade Óssea/uso terapêutico , Neoplasias Ósseas/secundário , Qualidade de Vida , Adulto , Idoso , Idoso de 80 Anos ou mais , Grupo com Ancestrais do Continente Asiático , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cooperação do Paciente , Inquéritos e Questionários , Estudos de Validação como Assunto , Adulto Jovem
Zhonghua Nan Ke Xue ; 9(7): 556-9, 2003 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-14628610


OBJECTIVE: To discuss spermatogenic and strengthening yang action of Baxian(BX) capsule and its possible mechanism. METHODS: Rats or mice were divided randomly into five groups: control group, Baxian groups(2.5 g/kg, 7.5 g/kg, 22.5 g/kg) and positive group. After the drug had been given for 14 days, the levels of serum testosterone and cortisol in the low-aged rats were measured by radioimmunoassay. The coefficient of the reproductive organs in rats, the quantity of sperm and interstitial cells in mice were investigated, and the change of the latency time of penis erection in castrated rats, the capture and ejaculation in normal rats were observed at the same time. RESULTS: BX capsule could obviously raise the concentration of serum testosterone and cortisol, and the coefficient of sex organ in rats, and increase the number of sperm and interstitial cells in mice. Also, it could improve the penis erection and sexual ability of the male rats. CONCLUSIONS: The spermatogenic and strengthening yang action of BX capsule could be attributed to the improvement of the actions of the axis of lower thalamus-hypophysis-sexual gland and the axis of lower thalamus-hypophysis-adrenal cortex.

Medicamentos de Ervas Chinesas/farmacologia , Genitália Masculina/efeitos dos fármacos , Hidrocortisona/sangue , Testosterona/sangue , Animais , Relação Dose-Resposta a Droga , Masculino , Camundongos , Camundongos Endogâmicos , Tamanho do Órgão/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley
Science ; 296(5565): 92-100, 2002 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-11935018


The genome of the japonica subspecies of rice, an important cereal and model monocot, was sequenced and assembled by whole-genome shotgun sequencing. The assembled sequence covers 93% of the 420-megabase genome. Gene predictions on the assembled sequence suggest that the genome contains 32,000 to 50,000 genes. Homologs of 98% of the known maize, wheat, and barley proteins are found in rice. Synteny and gene homology between rice and the other cereal genomes are extensive, whereas synteny with Arabidopsis is limited. Assignment of candidate rice orthologs to Arabidopsis genes is possible in many cases. The rice genome sequence provides a foundation for the improvement of cereals, our most important crops.

Genoma de Planta , Oryza/genética , Análise de Sequência de DNA , Arabidopsis/genética , Mapeamento Cromossômico , Cromossomos/genética , Biologia Computacional , Sequência Conservada , DNA de Plantas/genética , Bases de Dados de Ácidos Nucleicos , Grão Comestível/genética , Duplicação Gênica , Genes de Plantas , Genômica , Oryza/metabolismo , Oryza/fisiologia , Proteínas de Transporte de Fosfato/genética , Doenças das Plantas , Proteínas de Plantas/química , Proteínas de Plantas/genética , Estruturas Vegetais/genética , Sequências Repetitivas de Ácido Nucleico , Homologia de Sequência do Ácido Nucleico , Software , Sintenia , Fatores de Transcrição/genética