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1.
ACS Appl Mater Interfaces ; 14(36): 41215-41224, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36064349

RESUMO

Persistent luminescent materials (PLMs) are promising candidates for the anti-counterfeiting and information encryption field. However, ultraviolet (UV) excitation and visible emission are partially responsible for enabling information that has been encrypted to combat counterfeiting to be accessed by trial and error, resulting in imitation and information leakage. Here, we propose the possibility of controlling the persistent luminescent (PersL) emission spectra and its excitation light source with the use of band gap engineering, while obtaining X-ray exciting, not UV exciting UV PLM for advanced anti-counterfeiting and encryption application. Cationic substitution was used to adjust the width of the band gap of Lu(X)O4 (X = V, Nb, Ta, and P) from ∼4 to 9 eV. In addition, Bi3+ was introduced into the host as an emitter, which enabled the PersL emission spectra to be modulated from ∼550 to 230 nm. Among these PLMs, LuPO4:Bi3+ has unique optical properties. Under UV excitation, LuPO4:Bi3+ exhibits weak, inconspicuous visible down-conversion luminescence (DCL), without PersL ceasing once excitation is discontinued. Interestingly, LuPO4:Bi3+ displays UV PersL after X-ray excitation, and human eyes are insensitive to UV PersL, which requires specialized optical equipment to detect. A proof-of-concept assessment of LuPO4:Bi3+ for anti-counterfeiting and information encryption applications demonstrated its suitability in this regard.

2.
Nat Commun ; 13(1): 5339, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36096894

RESUMO

Zwitterionic hydrogels exhibit eminent nonfouling and hemocompatibility. Several key challenges hinder their application as coating materials for blood-contacting biomedical devices, including weak mechanical strength and low adhesion to the substrate. Here, we report a poly(carboxybetaine) microgel reinforced poly(sulfobetaine) (pCBM/pSB) pure zwitterionic hydrogel with excellent mechanical robustness and anti-swelling properties. The pCBM/pSB hydrogel coating was bonded to the PVC substrate via the entanglement network between the pSB and PVC chain. Moreover, the pCBM/pSB hydrogel coating can maintain favorable stability even after 21 d PBS shearing, 0.5 h strong water flushing, 1000 underwater bends, and 100 sandpaper abrasions. Notably, the pCBM/pSB hydrogel coated PVC tubing can not only mitigate the foreign body response but also prevent thrombus formation ex vivo in rats and rabbits blood circulation without anticoagulants. This work provides new insights to guide the design of pure zwitterionic hydrogel coatings for biomedical devices.


Assuntos
Hidrogéis , Microgéis , Animais , Hidrogéis/química , Cloreto de Polivinila , Coelhos , Ratos
3.
Pest Manag Sci ; 2022 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-36054174

RESUMO

BACKGROUND: Nucleopolyhedrovirus (NPV), one of the baculoviruses, is a promising biopesticide for pest control. Lepidopteran account for 70% of pests, therefore investigation on highly conserved genes associated with viral infections in the lepidopteran model, the silkworm, will serve as a valuable reference for improving the effectiveness of pest management. BmE74A is a member of the erythroblast transformation-specific (ETS) family of transcription factors in Bombyx mori, which we previously found to be highly conserved and closely associated with BmNPV. This study aimed to elucidate the role of BmE74A in viral infection. RESULTS: A significantly high expression of BmE74A in eggs indicated its important role in embryonic development, as did relatively high expressions in the hemolymph and midgut. Significant differences in BmE74A expression in different resistant strains after BmNPV infection suggested its involvement as a response to viral infection. Moreover, RNA interference (RNAi) and overexpression experiments confirmed the important role of BmE74A in promoting viral infection. BmNPV infection was significantly suppressed and enhanced by BmE74A knockdown and overexpression, respectively. Besides, BmE74A was found to regulate the expression of BmMdm2 and Bmp53. Furthermore, the binding of ETS, the functional domain of BmE74A, to occlusion-derived virus proteins was confirmed by far-western blotting, and four viral proteins that may interact with ETS proteins were identified by mass spectrometry. Similarly, a homolog of BmE74A in Spodoptera litura was also found to be involved in larval susceptibility to BmNPV. CONCLUSION: BmE74A promotes BmNPV proliferation by directly interacting with the virus, which may be related to the suppression of the p53 pathway. © 2022 Society of Chemical Industry.

4.
Artigo em Inglês | MEDLINE | ID: mdl-36165460

RESUMO

Underwater adhesion plays an essential role in soft electronics for the underwater interface. Although hydrogel-based electronics are of great interest, because of their versatility, water molecules prevent hydrogels from adhering to substrates, thus bottlenecking further applications. Herein, inspired by the barnacle proteins, MXene/PHMP hydrogels with strong repeatable underwater adhesion are developed through the random copolymerization of 2-phenoxyethyl acrylate, 2-methoxyethyl acrylate, and N-(2-hydroxyethyl) acrylamide with the presence of MXene nanosheets. The hydrogels are mechanically tough (elastic modulus of 32 kPa, fracture stress of 0.11 MPa), and 2-phenoxyethyl acrylate (PEA) with aromatic groups endows the hydrogel with nonswelling property and prevents water molecules from invading the adhesive interface, rendering the hydrogels an outstanding adhesive behavior toward various substrates (including glass, iron, polyethylene terephthalate (PET), porcine). Besides, dynamic physical interactions allow for instant and repeatable underwater adhesion. Furthermore, the MXene/PHMP hydrogels exhibit a high conductivity (0.016 S/m), fast responsiveness, and superior sensitivity as a strain sensor (gauge factor = 7.17 at 200%-500% strain) and pressure sensor (0.63 kPa-1 at 0-70 kPa). The underwater applications of bionic hydrogel-based sensors have been demonstrated, such as human motion, pressure sensing, and holding objects. It is anticipated that the instant and repeatable underwater adhesive hydrogel-based sensors extend the underwater applications of hydrogel electronics.

5.
J Biomed Inform ; 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36064114

RESUMO

The extraction of drug-drug interactions (DDIs) is an important task in the field of biomedical research, which can reduce unexpected health risks during patient treatment. Previous work indicates that methods using external drug information have a much higher performance than those methods not using it. However, the use of external drug information is time-consuming and resource-costly. In this work, we propose a novel method for extracting DDIs which does not use external drug information, but still achieves comparable performance. First, we no longer convert the drug name to standard tokens such as DRUG0, the method commonly used in previous research. Instead, full drug names with drug entity marking are input to BioBERT, allowing us to enhance the selected drug entity pair. Second, we adopt the Key Semantic Sentence approach to emphasize the words closely related to the DDI relation of the selected drug pair. After the above steps, the misclassification of similar instances which are created from the same sentence but corresponding to different pairs of drug entities can be significantly reduced. Then, we employ the Gradient Harmonizing Mechanism (GHM) loss to reduce the weight of mislabeled instances and easy-to-classify instances, both of which can lead to poor performance in DDI extraction. Overall, we demonstrate in this work that it is better not to use drug blinding with BioBERT, and show that GHM performs better than Cross-Entropy loss if the proportion of label noise is less than 30%. The proposed model achieves state-of-the-art results with an F1-score of 84.13% on the DDIExtraction 2013 corpus (a standard English DDI corpus), which fills the performance gap (4%) between methods that rely on and do not rely on external drug information.

6.
Neuropharmacology ; 220: 109259, 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36126726

RESUMO

Somatic symptom disorder (SSD), which occurs in about 5-7 percent of the adult population, involves heightened physical and emotional sensitivity to pain. However, its neural mechanism remains elusive and thus hinders effective clinical intervention. In this study, we employed chronic restraint stress (CRS)-induced hyperalgesia as a mouse model to investigate the neural mechanism underlying SSD and its pharmacological treatment. We found that CRS induced hyperactivity of anterior cingulate cortex (ACC), whereas chemogenetic inhibition of such hyperactivity could prevent CRS-induced hyperalgesia. Systematic application and ACC local infusion of fluoxetine alleviated CRS-induced hyperalgesia. Moreover, we found that fluoxetine exerted its anti-hyperalgesic effects through inhibiting the hyperactivity of ACC and upregulating 5-HT1A receptors. Our study thus uncovers the functional role of 5-HT signaling in modulating pain sensation and provides a neural basis for developing precise clinical intervention for SSD.

7.
Biosensors (Basel) ; 12(9)2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36140115

RESUMO

To establish rapid, high-sensitive, quantitative detection of ACP residues in vegetables. A 1G2 cell clone was selected as the most sensitive for anti-ACP antibody production following secondary immunization, cell fusion, and screening. The affinity of the 1G2 antibody to each of the four coating agents (imidacloprid-bovine serum albumin [BSA], thiacloprid-BSA, imidaclothiz-BSA, and ACP-BSA) was determined using a 20 min enzyme-linked immunosorbent assay (ELISA). The half maximal inhibitory concentration (IC50) was 0.51-0.62 ng/mL, showing no significant difference in affinity to different antigens. However, we obtained IC50 values of 0.58 and 1.40 ng/mL on the linear regression lines for 1G2 anti-ACP antibody/imidacloprid-BSA and 1G2 anti-ACP antibody/thiacloprid-BSA, respectively, via quantum dot (QD)-based immunochromatography. That is, the 1G2 antibody/imidacloprid-BSA pair (the best combination) was about three times more sensitive than the 1G2 antibody/thiacloprid-BSA pair in immunochromatographic detection. The best combination was used for the development of an 8 min chromatographic paper test. With simple and convenient sample pretreatment, we achieved an average recovery of 75-117%. The coefficient of variation (CoV) was <25% for all concentrations tested, the false-positive rate was <5%, the false-negative rate was 0%, and the linear range of the method was 50-1800 µg/kg. These performance metrics met the ACP detection standards in China, the European Union (EU), and the United States (US). In summary, in this study, we established an 8 min QD-based immunochromatographic stripe for the rapid and accurate quantitative determination of ACP residues in vegetables.


Assuntos
Soroalbumina Bovina , Verduras , Anticorpos , Ensaio de Imunoadsorção Enzimática/métodos , Neonicotinoides , Nitrocompostos , Tiazinas
8.
Int J Biol Macromol ; 221: 183-192, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36067846

RESUMO

An intelligent colorimetric film was developed for the quality detection of tilapia fillets using bacterial cellulose (BC) as a substrate in combination with pelargonidin (Pg), cyanidin (Cy), and delphinium (Dp). The color of the BC-Pg-Cy-Dp film and Pg-Cy-Dp solution changed from rosy to blue-violet at pH 3-10. The mechanical and antioxidant properties of the film were improved after the addition of Pg-Cy-Dp. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that a new hydrogen bond might be formed between the cellulose chain and the anthocyanin molecule, which increased the density of the film. The BC-Pg-Cy-Dp film displayed a large color difference from rosy to blue-violet when applied to tilapia fillet storage. The changes of K values indicated a good linear relationship with the change in ∆E at 4 °C and 25 °C. In the actual cold chain, the color of the film changed from rosy to purple, which could be identified by the naked eye and indicated that the fish were in the first fresh stage. Thus, the BC-Pg-Cy-Dp film can be used as an intelligent packaging film during storage to monitor the freshness of fish by the naked eye.

9.
Sensors (Basel) ; 22(15)2022 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-35957269

RESUMO

In this work, we reported a rapid and sensitive fluorescence assay in homogenous solution for detecting organophosphorus pesticides by using tetramethylrhodamine (TAMRA)-labeled aptamer and its complementary DNA (cDNA) with extended guanine (G) bases. The hybridization of cDNA and aptamer drew TAMRA close to repeated G bases, then the fluorescence of TAMRA was quenched by G bases due to the photoinduced electron transfer (PET). Upon introducing the pesticide target, the aptamer bound to pesticide instead of cDNA because of the competition between pesticide and cDNA. Thus, the TAMRA departed from G bases, resulting in fluorescence recovery of TAMRA. Under optimal conditions, the limits of detection for phorate, profenofos, isocarbophos, and omethoate were 0.333, 0.167, 0.267, and 0.333 µg/L, respectively. The method was also used in the analysis of profenofos in vegetables. Our fluorescence design was simple, rapid, and highly sensitive, which provided a means for monitoring the safety of agricultural products.


Assuntos
Aptâmeros de Nucleotídeos , Praguicidas , Aptâmeros de Nucleotídeos/genética , DNA Complementar , Fluorescência , Compostos Organofosforados/análise , Praguicidas/análise
10.
Mikrochim Acta ; 189(9): 317, 2022 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-35930068

RESUMO

MXene@Au as the base and Au@SiO2 as signal amplification factor were used for constructing an ultrasensitive "on-off" electrochemiluminescence (ECL) biosensor for the detection of Pb2+ in water. The use of MXene@Au composite provided a good interface environment for the loading of tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy)32+) on the electrode. Based on resonance energy transfer, the Au (core) SiO2 (shell) (Au@SiO2) nanoparticles stimulate electron transport and promote tripropylamine (TPrA) oxidation. The luminescence effect of Au@SiO2 was five times that of AuNPs and SiO2 nanomaterials alone, and the ECL intensity was greatly improved. In addition, Pb2+ activated the aptamer to exert its endonuclease activity, which realized the signal cycle amplification in the process of Pb2+ detection. When Pb2+ was added, the ECL signal weakened, and the Pb2+ concentration was detected according to the decreased ECL intensity. Under optimized experimental conditions, this aptamer sensor for Pb2+ has a wide detection range (0.1 to 1 × 106 ng L-1) and a low detection limit (0.059 ng L-1). The relative standard deviation (RSD) of the sensor is 0.39-0.99%, and the recovery of spiked standard is between 90.00 and 125.70%. The sensor shows good selectivity and high sensitivity in actual water sample analysis. This signal amplification strategy possibly provides a new method for the detection of other heavy metal ions and small molecules.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Técnicas Biossensoriais/métodos , Endonucleases , Ouro , Íons , Chumbo , Propilaminas , Dióxido de Silício , Água
11.
Biosensors (Basel) ; 12(8)2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-36005022

RESUMO

Herein, a novel electrochemical aptasensor using a broad-spectrum aptamer as a biorecognition element was constructed based on a screen-printed carbon electrode (SPCE) for simultaneous detection of aminoglycoside antibiotics (AAs). The ordered mesoporous carbon (OMC) was firstly modified on 2D Ti3C2 MXene. The addition of OMC not only effectively improved the stability of the aptasensor, but also prevented the stacking of Ti3C2 sheets, which formed a good current passage for signal amplification. The prepared OMC@Ti3C2 MXene functioned as a nanocarrier to accommodate considerable aptamers. In the presence of AAs, the transport of electron charge on SPCE surface was influenced by the bio-chemical reactions of the aptamer and AAs, generating a significant decline in the differential pulse voltammetry (DPV) signals. The proposed aptasensor presented a wide linear range and the detection limit was 3.51 nM. Moreover, the aptasensor, with satisfactory stability, reproducibility and specificity, was successfully employed to detect the multi-residuals of AAs in milk. This work provided a novel strategy for monitoring AAs in milk.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Aminoglicosídeos , Animais , Antibacterianos , Carbono , Técnicas Eletroquímicas , Eletrodos , Limite de Detecção , Leite , Reprodutibilidade dos Testes , Titânio
12.
Food Chem ; 397: 133838, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-35944333

RESUMO

The bioelectronic tongues based on taste receptors have been emerging with human-like taste perception. However, the practical applications of the receptor-based biosensors were restricted by their narrow and low dynamic ranges. Here, a novel immobilization strategy based on AuNPs@ZIF-8/Ti3C2 MXene was developed to immobilize the umami ligand binding domain (T1R1-VFT), to fabricate an umami biosensor for umami substances detection. Through the synergic effect of AuNPs@ZIF-8 and Ti3C2 MXene, the capacity to load T1R1-VFT was effectively increased, and the response signal was also amplified by approximately 3 times. The proposed biosensor showed an ultrawide dynamic range of 10-11-10-3 M, and a high upper limit of detection, which was closer to the human taste threshold and suitable for detecting foods rich in umami substances. Additionally, the biosensor was successfully applied to detect real samples and analyze the synergistic effects of binary umami substances.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Ouro , Humanos , Receptores Acoplados a Proteínas G/metabolismo , Paladar , Titânio/química
13.
J Hazard Mater ; 440: 129707, 2022 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-35986944

RESUMO

For the visual detection of four organophosphorus pesticides (OPs), a colorimetric aptasensor was developed based on aptamer-mediated bimetallic metal-organic frameworks (MOFs) nano-polymers. Fe-Co magnetic nanoparticles (MNPs) and Fe-N-C nanozymes were prepared based on pyrolytic reaction, and were labeled with broad spectrum aptamers and complementary chains of organophosphorus pesticides respectively. The hybridization of aptamers and complementary chains led to the formation of nano-polymers. In the presence of target pesticides, they competed with complementary chains for aptamers on Fe-Co MNPs, resulting in a large number of Fe-N-C nanozymes signal labels being released into the supernatant. Fe-N-C nanozymes showed similar activity to peroxidase and catalyzed the 3,3',5,5'-tetramethylbenzidine-hydrogen peroxide (TMB-H2O2) color system to turn the solution blue-green under mild conditions. The magnetic probes had good selectivity and sensitivity, and were easily separated by magnetic absorption. The sensor functioned well under optimal conditions, demonstrating good stability and specificity for four pesticides: phorate, profenofos, isocarbophos and omethoate, and the detection limits of four pesticides were as low as 0.16 ng/mL, 0.16 ng/mL, 0.03 ng/mL and 1.6 ng/mL respectively, and the recovery rate of OPs residue in vegetable samples was satisfactory. The work described here provided a simple, rapid and sensitive way to construct a biosensor.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Estruturas Metalorgânicas , Praguicidas , Aptâmeros de Nucleotídeos/química , Colorimetria/métodos , Peróxido de Hidrogênio , Limite de Detecção , Estruturas Metalorgânicas/química , Compostos Organofosforados , Peroxidases , Praguicidas/análise , Forato
14.
Acta Biomater ; 151: 290-303, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-35995406

RESUMO

Zwitterionic polymers have shown promising results in non-fouling and preventing thrombosis. However, the lack of controlled surface coverage hinders their application for biomedical devices. Inspired by the natural biological surfaces, a facile zwitterionic microgel-based coating strategy is developed by the co-deposition of poly (sulfobetaine methacrylate-co-2-aminoethyl methacrylate) microgel (SAM), polydopamine (PDA), and sulfobetaine-modified polyethyleneimine (PES). The SAMs were used to construct controllable morphology by using the PDA combined with PES (PDAS) as the intermediate layer, which can be easily modulated via adjusting the crosslinking degree and contents of SAMs. The obtained SAM/PDAS coatings exhibit high anti-protein adhesive properties and can effectively inhibit the adhesion of cells, bacteria, and platelet through the synergy of high deposition density and controllable morphology. In addition, the stability of SAM/PDAS coating is improved owing to the anchoring effects of PDAS to substrate and SAMs. Importantly, the ex vivo blood circulation test in rabbits suggests that the SAM/PDAS coating can effectively decrease thrombosis without anticoagulants. This study provides a versatile coating method to address the integration of zwitterionic microgel-based coatings with high deposition density and controllable morphology onto various substrates for wide biomedical device applications. STATEMENT OF SIGNIFICANCE: Thrombosis is a major cause of medical device implantation failure, which results in significant morbidity and mortality. In this study, inspired by natural biological surfaces (fish skin and vascular endothelial layer) and the anchoring ability of mussels, we report a convenient and efficient method to firmly anchor zwitterionic microgels using an oxidative co-deposition strategy. The prepared coating has excellent antifouling and antithrombotic properties through the synergistic effect of physical morphology and chemical composition. This biomimetic surface engineering strategy is expected to provide new insights into the clinical problems of blood-contacting devices related to thrombosis.


Assuntos
Microgéis , Animais , Anticoagulantes , Betaína/análogos & derivados , Fibrinolíticos , Metacrilatos , Polietilenoimina/farmacologia , Polímeros/química , Coelhos , Propriedades de Superfície
15.
Bioelectrochemistry ; 148: 108227, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-35973324

RESUMO

In this work, an interference-resistant electrochemical aptasensor that could detect profenofos in vegetables was constructed based on complexes of graphene oxide and polyaniline (GO@PANI) and gold nanoparticles-tetrahedral DNA nanostructure (Au-TDN). Compared with a single chain aptamer, the tetrahedral DNA nanostructure is highly stable and allows the aptamer on this structure to stand in a highly ordered position on an electrode surface. Moreover, the AuNPs are biocompatible and can protect the activity of the aptamer, which can improve the assembly success rate of Au-TDN. Besides, the conductivity of PANI had been tremendously enhanced thanks to the existence of GO, which improved the dispersion of PANI. The GO@PANI was prepared by a chemical synthesis method, which had a large surface area and was able to adsorb many Au-TDN. Under optimal working parameters, the constructed aptasensor exhibited good electrochemical sensing performance with a detection limit of 10.50 pg/mL and a linear range of 1.0 × 102-1.0 × 107 pg/mL. In addition, it was employed in detecting profenofos in vegetables with a good recovery rate of 90.41-116.37 %. More importantly, the aptasensor also has excellent stability and high selectivity. This study provides a promising method to avoid interference in the detection of profenofos by sensors.

16.
J Affect Disord ; 315: 190-197, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35908604

RESUMO

INTRODUCTION: The aims of our study are: i) to explore whether plasma levels of BDNF/GDNF are valuable in the diagnosis of first-episode depression; ii) to discuss whether there is an association between peripheral plasma levels of BDNF/GDNF and patients' depression severity and cognitive dysfunction; iii) to explore the association between plasma levels of BDNF/GDNF and the effectiveness of antidepressant treatment. METHODS: Ninety patients with first-episode unmedicated MDD and healthy controls were recruited. MDD patients were treated with antidepressant medication for 8 weeks. Patients were assessed for clinical symptoms using HDRS-17 and HAMA-14. Social and neurocognitive functioning of all subjects was assessed at baseline using the Functional Assessment Test Short Form (FAST) and the MATRICS Consensus Cognitive Battery (MCCB). At the same time, peripheral venous blood was drawn from all subjects for BDNF/GDNF peripheral plasma level analysis at baseline and after 8 weeks of treatment. RESULTS: The baseline BDNF/GDNF levels in MDD patients were significantly lower than that in healthy controls. The area under ROC curve (AUC) of baseline plasma BDNF and GDNF levels predicting MDD was 0.776 (95 % CI: 0.705-0.846, p < 0.001) and 0.864 (95 % CI: 0.808-0.920, p < 0.001), respectively. The baseline GDNF level (beta = 0.425, p = 0.001), the autonomy score of FAST (beta = -0.247, p = 0.037) and BACS-SC score of MCCB (beta = 0.323, p = 0.039) were predictors of HDRS-17 reduction rate after 8 weeks' antidepressant treatment. LIMITATIONS: A longer follow-up period than 8 weeks may make the results more convincing, and the sample size of this study is still insufficient. CONCLUSION: The decreased plasma levels of BDNF and GDNF are strong indicators for predicting the occurrence of MDD. This preliminary finding highlighted the value of GDNF plasma concentrations in the diagnosis of MDD and the prognosis of antidepressant treatment.


Assuntos
Transtorno Depressivo Maior , Antidepressivos/uso terapêutico , Fator Neurotrófico Derivado do Encéfalo , Transtorno Depressivo Maior/diagnóstico , Transtorno Depressivo Maior/tratamento farmacológico , Fator Neurotrófico Derivado de Linhagem de Célula Glial , Humanos , Prognóstico
17.
Biosensors (Basel) ; 12(8)2022 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-35892457

RESUMO

To produce a sensitive monoclonal antibody (mAb) for the simultaneous detection of carbofuran, benfuracarb, carbosulfan and 3-hydroxy-carbofuran, 2,3-dihydro-2,2-dimethyl-7-benzofuranmethanamine (DDB) was conjugated to bovine serum albumin (BSA) to prepare the immunogen DDB-BSA and mice were immunized. Coating antigens were prepared by conjugating DDB and 5-methoxy-2,3-dihydrobenzofuran-3-acetic acid (MDA) to BSA and ovalbumin (OVA), respectively. Furthermore, the effect of different antibody-antigen pairs on the sensitivity of ELISA and LFIA methods for the detection of carbofuran was investigated. After the immunization, a high-affinity mAb 13C8 was obtained. The ability of the coating antigen to compete with carbofuran for binding antibodies was found to be significantly different between ELISA and LFIA methods. With the antibody-antigen pair 13C8-MDA-OVA, the IC50 values of the ELISA and QD-LFIA methods for carbofuran were 0.18 ng/mL and 0.67 ng/mL, respectively. The cross-reactivity (CR) values of the two methods for benfuracarb, carbosulfan and 3-hydroxy-carbofuran ranged from 72.0% to 83.7%, while, for other carbamate pesticides, the CR values were less than 1%. The spiked recoveries of carbofuran in vegetables by the QD-LFIA method were 83-111%, with a coefficient of variation below 10%, and the test results of the actual samples were consistent with the HPLC-MS method. Overall, this study provides key materials for the development of immunoassays for carbofuran and its analogues, and the antibody-antigen pair selection strategy established in this study provides useful insights for the development of sensitive immunoassays for other compounds.


Assuntos
Carbofurano , Praguicidas , Animais , Anticorpos Monoclonais , Antígenos/química , Carbofurano/análise , Ensaio de Imunoadsorção Enzimática/métodos , Imunoensaio/métodos , Camundongos , Praguicidas/análise
18.
PLoS One ; 17(7): e0270840, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35797274

RESUMO

Bombyx mori is an important economic insect, its economic value mainly reflected in the silk yield. The major functional genes affecting the silk yield of B. mori have not been determined yet. Bombyx mori vacuolar protein sorting-associated protein 13d (BmVps13d) has been identified, but its function is not reported. In this study, BmVps13d protein shared 30.84% and 34.35% identity with that of in Drosophila melanogaster and Homo. sapiens, respectively. The expressions of BmVps13d were significantly higher in the midgut and silk gland of JS (high silk yield) than in that of L10 (low silk yield). An insertion of 9 bp nucleotides and two deficiencies of adenine ribonucleotides in the putative promoter region of BmVps13d gene in L10 resulted in the decline of promoter activity was confirmed using dual luciferase assay. Finally, the functions of BmVps13d in B. mori were studied using the CRISPR/Cas9 system, and the mutation of BmVps13d resulted in a 24.7% decline in weight of larvae, as well as a 27.1% (female) decline and a 11.8% (male) decline in the silk yield. This study provides a foundation for studying the molecular mechanism of silk yield and breeding the silkworm with high silk yield.


Assuntos
Bombyx , Genes de Insetos , Proteínas de Insetos , Seda , Animais , Bombyx/química , Bombyx/genética , Bombyx/metabolismo , Proteínas de Drosophila , Drosophila melanogaster/química , Feminino , Genes de Insetos/genética , Humanos , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular , Larva/anatomia & histologia , Masculino , Mutação , Regiões Promotoras Genéticas/genética , Proteínas , Seda/biossíntese
19.
Front Psychiatry ; 13: 932235, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35815047

RESUMO

Background: Brain-derived neurotrophic factor (BDNF) is a candidate for susceptibility locus of Panic disorder (PD). However, the findings about the role of the BDNF Val66Met variant in PD were not consistent. Till now, the relationship between BDNF Val66Met polymorphism and anxiety-related traits in PD patients has been rarely explored. This study aimed to explore the relationship among BDNF Val66Met polymorphism, plasma BDNF level and anxiety-related trait in Chinese PD patients. Method: This multi-center study included 116 PD patients and 99 health controls. We detected single-nucleotide polymorphism (SNP) of BDNF rs6265 (Val66Met) and BDNF plasma level in the two groups. In addition, PD patients were administered the State-Trait Anxiety Inventory (STAI), Panic Disorder Severity Scale-Chinese Version (PDSS-CV) and Hamilton Anxiety Rating Scale (HAMA-14). Quantitative comparison of the differences of BDNF concentration among subjects with different genotypes and association between BDNF Val66Met genotype and trait anxiety were performed. Results: There were no significant differences in the genotype frequency (p = 0.79) or allele frequency (p = 0.88) between PD patients and health controls. BDNF plasma levels of PD patients were significantly lower than those in control group (p = 0.003). BDNF plasma levels of the Met/Met genotype were significantly lower than those of Val/Met genotype in PD patients (p = 0.033). PD patients carried Met/Met genotype showed significantly higher scores in STAI trait compared to those carried Val/Val genotype (p = 0.045) and Val/Met genotype (p = 0.018). STAI trait scores of PD patients with agoraphobia were significantly higher than those of patients without agoraphobia (p < 0.05). The ANCOVA showed that the dependent variable STAI trait score was significantly affected by factor "genotype" (Val/Val, Val/Met, Met/Met, p = 0.029), and covariate "agoraphobia" (p = 0.008). In this model, 11.5% of the variance of the STAI trait score was explained by the BDNF genotype. Contrast analysis showed STAI trait scores of Met/Met subjects were significantly higher than those of Val/Met (p = 0.018) and Val/Val individuals (p = 0.045). Conclusion: We found that anxiety trait was associated with the BDNF polymorphism in PD patients. BDNF Met/Met genotype may decrease plasma BDNF level and increase trait anxiety in panic disorder.

20.
Anal Bioanal Chem ; 414(20): 6127-6137, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35804073

RESUMO

In order to address the widespread concerns with food safety such as adulteration and forgery in the edible oil field, this study developed a fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody in a homogeneous solution system for determination of capsaicinoids in gutter cooking oil by using chemically stable capsaicinoids as an adulteration marker. The prepared fluoresceinthiocarbamyl ethylenediamine (EDF) was coupled with capsaicinoid hapten C, and the synthesized tracer was purified by thin-layer chromatography (TLC) and showed good binding to the monoclonal antibody CPC Ab-D8. The effects of concentration of tracer and recognition components, type and pH of buffer and incubation time on the performance of FPIA were studied. The linear range (IC20 to IC80) was 3.97-97.99 ng/mL, and the half maximal inhibitory concentration (IC50) was 19.73 ng/mL, and the limit of detection (LOD) was 1.56 ng/mL. The recovery rates of corn germ oil, soybean oil and peanut blend oil were in the range of 94.7-132.3%. The experimental results showed that the fluorescence polarization detection system could realize the rapid detection of capsaicinoids, and had the potential to realize on-site identification of gutter cooking oil. As a universal monoclonal antibody, CPC Ab-D8 can also specifically identify capsaicin and dihydrocapsaicin, so the proposed method can be used to quickly monitor for the presence of gutter cooking oil in normal cooking oil.


Assuntos
Culinária , Alimentos , Anticorpos Monoclonais , Imunoensaio de Fluorescência por Polarização/métodos , Limite de Detecção
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