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1.
Artigo em Inglês | MEDLINE | ID: mdl-33538049

RESUMO

Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve [y = 0.1311x + 0.5386; R² = 0.9707] with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer.

2.
Commun Biol ; 4(1): 213, 2021 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-33594251

RESUMO

The mucosa microenvironment is critical for intestinal stem cell self-renewal and reconstruction of the epithelial barrier in inflammatory bowel disease (IBD), where the mechanisms underlying cross-talk between intestinal crypts and the microenvironment remain unclear. Here, we firstly identified miR-494-3p as an important protector in colitis. miR-494-3p levels were decreased and negatively correlated with the severity in human IBD samples, as well as in colitis mice. In colitis crypts, a notable cytokine-cytokine receptor, miR-494-3p-targeted EDA2R and the ligand EDA-A2, suppressed colonic stemness and epithelial repair by inhibiting ß-catenin/c-Myc. In differentiated IECs, miR-494-3p inhibits macrophage recruitment, M1 activation and EDA-A2 secretion by targeting IKKß/NF-κB in colitis. A miR-494-3p agomir system notably ameliorated the severity of colonic colitis in vivo. Collectively, our findings uncover a miR-494-3p-mediated cross-talk mechanism by which macrophage-induced intestinal stem cell impairment aggravates intestinal inflammation.

3.
IEEE Trans Cybern ; PP2021 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33531331

RESUMO

Knowledge tracing is an important research topic in student modeling. The aim is to model a student's knowledge state by mining a large number of exercise records. The dynamic key-value memory network (DKVMN) proposed for processing knowledge tracing tasks is considered to be superior to other methods. However, through our research, we have noticed that the DKVMN model ignores both the students' behavior features collected by the intelligent tutoring system (ITS) and their learning abilities, which, together, can be used to help model a student's knowledge state. We believe that a student's learning ability always changes over time. Therefore, this article proposes a new exercise record representation method, which integrates the features of students' behavior with those of the learning ability, thereby improving the performance of knowledge tracing. Our experiments show that the proposed method can improve the prediction results of DKVMN.

4.
Sleep Breath ; 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33415654

RESUMO

BACKGROUND AND OBJECTIVE: Obstructive sleep apnea (OSA) is associated with hypertension, psychological impairment, neurocognitive dysfunction, and poor quality of sleep. Continuous positive airway pressure (CPAP) has been confirmed to effectively improve OSA, while the effects of supplemental oxygen therapy on OSA have still remained controversial. This meta-analysis aimed to compare the effects of supplemental oxygen therapy and CPAP on patients with OSA. METHODS: PubMed, Cochrane library, EMBASE, and Web of Science databases were systematically searched from inception until April 2020. Randomized controlled trials (RCTs) that compared the effects of supplemental oxygen therapy and CPAP on patients with OSA were selected without language restriction. RESULTS: In this meta-analysis, 8 RCTs that involved 887 patients were found eligible for further analyses. Pooled data showed that there was no significant difference in improving nocturnal oxygen saturation (SpO2) level (95% confidence interval (CI) = - 1.17 to 1.53) or symptoms of depression (95%CI = - 0.69 to 1.19) between supplemental oxygen therapy and CPAP. Supplemental oxygen therapy was found less effective in reducing apnea-hypopnea index (AHI), time of SpO2 < 90%, blood pressure, and improving quality of sleep compared with CPAP. A subgroup analysis based on flow rate of oxygen indicated that the effects of supplemental oxygen therapy on blood pressure significantly differed. Furthermore, an improvement in overall time of SpO2 < 90% was correlated to duration of supplemental oxygen therapy. CONCLUSIONS: CPAP is clinically effective for the treatment of patients with OSA. However, supplemental oxygen therapy can be cautiously used for improving nocturnal hypoxia and symptoms of depression when CPAP is not acceptable or not tolerated. Supplemental oxygen therapy is a promising option to alleviate partial disorders of OSA. Further studies need to focus on flow rate of oxygen and duration of supplemental oxygen therapy.

5.
Bioorg Med Chem ; 32: 116003, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33461148

RESUMO

Progesterone receptor (PR) antagonists have been found to be effective for treating certain human cancers. However, the steroidal structure of PR antagonists could bind to other hormone receptors, thus leading to serious side effects. On the other hand, non-steroidal PR antagonists have rarely been evaluated for their anti-cancer efficacy. Therefore, identifying novel non-steroidal PR antagonists possessing potent anti-cancer efficacy would be an attractive project to pursue. In this study, we presented a new metal-free oxidative CH arylation method to rapidly synthesize a series of 6-aryl-6H-benzo[c]chromene derivatives. Multiple cancer cell lines were used for their anti-cancer activity screening. An extensive analysis of structure-activity relationships (SAR) of the derivatives revealed that compounds 32 and 34 markedly inhibited the proliferation of MCF-7 cells with IC50 values of 6.32 ± 0.52 µM and 5.71 ± 0.49 µM, respectively. Further investigation indicated that derivatives 32 and 34 could elevate the expression of p21 and decrease the expressions of CDK4 and cyclin D1, leading to cell cycle arrest at G0/G1 phase. In addition, derivatives 32 and 34 could induce apoptosis of MCF-7 cells in both dose- and time-dependent manners by activation of p53 pathway, i.e., activation of Cleaved Caspase-3, p53 and P-p53 as well as elevation of the Bax/Bcl-2 ratio. Docking of derivatives 32 and 34 into a PR homology model exhibited potent PR antagonistic activity indicating the 6-aryl-6H-benzo[c]chromene derivatives are promising PR antagonists. We envisioned that derivatives 32 and 34 might be potential anti-cancer drug candidates as novel therapeutic treatment for breast cancer.

6.
Sci Total Environ ; 758: 143947, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33338791

RESUMO

We studied the spatial variations of six volatile halocarbons (VHCs), namely, iodomethane (CH3I), chloroform (CHCl3), tetrachloroethylene (C2Cl4), bromodichloromethane (CHBrCl2), dibromomethane (CH2Br2), and carbon tetrachloride (CCl4), and the environmental influencing factors involved in the cycling of VHCs in the upper ocean (0-500 m) off the Northern Antarctic Peninsula (NAP) during the summer of 2018. About 5%-10% of the total biogenic VHCs in the upper ocean were accumulated in the assemblage layer (AL) with high chlorophyll a. However, higher VHCs levels were observed in the dicothermal layer (DL) compared with the AL because of the preservation from winter and production from dinoflagellates and chlorophytes. Owing to the co-existence occurrence of sharp seasonal pycnocline and thick permanent pycnocline, DL could be an important VHCs reservoir in the upper water column during summer. In response to melting of sea ice and glacier, decreased salinity was responsible for ca. 50% of the variation in the CH2Br2 and CCl4 concentrations, which corresponded with increased CH2Br2 and CCl4 concentrations in the less saline water mass. Anthropogenic CCl4 was found with an average concentration of 44.9 pmol/L, and there was a strong positive relationship between CCl4 and CHCl3 in the upper water, indicating their similar source of pollutant transport caused by anthropogenic activities. Calculated sea-to-air fluxes of CCl4, C2Cl4, CHBrCl2, and CH2Br2 averaged 478.7, 93.7, 33.7, and 61.8 nmol/(m2·d) in summer, respectively, indicating that the waters off the NAP are important sources of VHCs for the atmosphere and exert potentially adverse impacts on the Antarctica ozone depletion.

7.
J Hazard Mater ; 401: 123794, 2021 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-33113737

RESUMO

In this work, an ultrasensitive and selective electrochemiluminescence (ECL) aptasensor with Au-tetrahedral aptamer nanostructure (Au-TAN) for acetamiprid detection was developed, which employed luminescence property of luminol and hydrogen peroxide (H2O2) as a co-reactant to apply the prepared Au-TAN to the luminescence systems. Au-TAN was prepared to modify an electrode surface via an Au-S bond to form a stable tetrahedral nanostructure. Fixed on the surface of the working electrode, Au-TAN could not only enhance the function of the aptamer but also boost the sensing performance. At the same time, Au nanoparticles (AuNPs) of the Au-TAN could also catalyze H2O2, thereby enhancing the luminescence performance of this aptasensor. The pH of the buffer solution, the concentration of H2O2 and the concentration of Au-TAN were optimized. Under the optimal conditions, the aptasensor had a detection limit of 0.0576 pM (S/N = 3), which was lower than those of other aptasensors for acetamiprid detection. Moreover, the weak alkaline environment explored in the experiment could expand its application range. Above all, the proposed method presented a high accuracy and sensitivity.

8.
Ann Transplant ; 25: e926857, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33258806

RESUMO

BACKGROUND This study aimed to assess the psychosocial status (mood, sleep quality, and activities of daily living) of candidates on an orthotopic liver transplantation (OLT) waiting list and to identify the association between psychosocial factors and MELD score in end-stage liver disease (ESLD). MATERIAL AND METHODS Fifty-three OLT waiting list candidates completed 4 scales (Hamilton Rating Scale for Depression [HAMD-17], Hamilton Anxiety Rating Scale [HAM-A], Pittsburgh Sleep Quality Index [PSQI], Activities of Daily Living Scale [ADL]) to assess their affective status, sleep quality, and daily living ability. Candidates were divided into 2 groups, the high MELD score group (MELD score ≥15) and the low MELD score group (MELD score.

9.
Biochem Pharmacol ; 183: 114337, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33186592

RESUMO

The opening of endothelial small-conductance calcium-activated potassium channels (KCa2.3) is essential for endothelium-dependent hyperpolarization (EDH), which predominantly occurs in small resistance arteries. Adenosine monophosphate-activated protein kinase (AMPK), an important metabolic regulator, has been implicated in regulating endothelial nitric oxide synthase activity. However, it was unclear whether AMPK regulated endothelial KCa2.3-mediated EDH-type vasodilation. Using bioinformatics analysis and myograph system, we investigated the regulation by AMPK of KCa2.3 in human umbilical vein endothelial cells (HUVECs) or mouse second-order mesenteric resistance arteries. In HUVECs, AMPK activation either by activators (AICAR, A769662 and MK-8722) or expression of the constitutively active form of AMPK significantly upregulated KCa2.3 expression. Such effects were abolished by AMPK inhibitor (compound C) or AMPK α1-/α2-siRNA, extracellular-signal-regulated-kinase 5 (ERK5) inhibitor (ERK5-IN-1), and specific siRNA to myocyte-enhancer factor 2 (MEF2) or krüppel-like factor 2/4 (KLF2/4). KCa2.3 expression was significantly reduced in mesenteric resistance arteries in AMPKα2 knockout mice when compared with littermate control mice. Furthermore, in high-fat diet fed mice, 2-week treatment with AICAR restored endothelial KCa2.3 expression in mesenteric resistance arteries with improved endothelial dysfunction. Our results demonstrate that activation of AMPK upregulates KCa2.3 channel expression through the ERK5-MEF2-KLF2/4 signaling pathway in vascular endothelium, which contributes to benefits through KCa2.3-mediated EDH-type vasodilation in mesenteric resistance arteries.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33161490

RESUMO

Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs to form a gold-labeled probe (AuNPs-Abs), which enhances the effective binding of antibodies to nanomaterials. Prussian blue (PB) was added to electrodeposition solution to enhance the conductivity, resulting in superior electrochemical performance. The AuNP-Abs-PB composite film was prepared by electrodeposition on the electrode surface to improve the anti-interference ability and stability of the immunosensor. Under the optimal experimental conditions, the immunosensor had a wide detection range (IC20-IC80: 1.82 × 10-3-3.29 × 104 ng/mL) and high sensitivity. Most importantly, it was simple to be prepared and could be used to detect multiple OPs.

11.
Sensors (Basel) ; 20(22)2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33217912

RESUMO

Immunoassay has the advantages of high sensitivity, high specificity, and simple operation, and has been widely used in the detection of mycotoxins. For several years, time-resolved fluorescence immunochromatography (TRFIA) paper-based sensors have attracted much attention as a simple and low-cost field detection technology. However, a traditional TRFIA paper-based sensor is based on antibody labeling, which cannot easily meet the current detection requirements. A second antibody labeling method was used to amplify the fluorescence signal and improve the detection sensitivity. Polystyrene fluorescent microspheres were combined with sheep anti-mouse IgG to prepare fluorescent probes (Eu-IgGs). After the probe fully reacted with the antibody (Eu-IgGs-Abs) in the sample cell, it was deployed on the paper-based sensor using chromatography. Eu-IgGs-Abs that were not bound to the target were captured on the T-line, while those that were bound were captured on the C-line. The paper-based sensor reflected the corresponding fluorescence intensity change. Because a single molecule of the deoxynivalenol antibody could bind to multiple Eu-IgGs, this method could amplify the fluorescence signal intensity on the unit antibody and improve the detection sensitivity. The working standard curve of the sensor was established under the optimum working conditions. It showed the lower limit of detection and higher recovery rate when it was applied to actual samples and compared with other methods. This sensor has the advantages of high sensitivity, good accuracy, and good specificity, saving the amount of antibody consumed and being suitable for rapid field detection of deoxynivalenol.

12.
Molecules ; 25(21)2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33143016

RESUMO

Andrographolide is a labdene diterpenoid with potential applications against a number of viruses, including the mosquito-transmitted dengue virus (DENV). In this study, we evaluated the anti-viral activity of three 14-aryloxy analogues (ZAD-1 to ZAD-3) of andrographolide against Zika virus (ZIKV) and DENV. Interestingly, one analogue, ZAD-1, showed better activity against both ZIKV and DENV than the parental andrographolide. A two-dimension (2D) proteomic analysis of human A549 cells treated with ZAD-1 compared to cells treated with andrographolide identified four differentially expressed proteins (heat shock 70 kDa protein 1 (HSPA1A), phosphoglycerate kinase 1 (PGK1), transketolase (TKT) and GTP-binding nuclear protein Ran (Ran)). Western blot analysis confirmed that ZAD-1 treatment downregulated expression of HSPA1A and upregulated expression of PGK1 as compared to andrographolide treatment. These results suggest that 14-aryloxy analogues of andrographolide have the potential for further development as anti-DENV and anti-ZIKV agents.

13.
Inflamm Res ; 69(12): 1283, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33052416

RESUMO

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s00011-020-01406-1.

14.
J BUON ; 25(4): 2017-2022, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33099947

RESUMO

PURPOSE: This study aimed to observe the effect of neo-adjuvant chemotherapy on the improvement of oral cancer patients and investigate the predictive value of miR-182 on its efficacy. METHODS: A total of 143 patients with advanced oral cancer admitted to Yidu Central Hospital of Weifang from September 2015 to July 2017 formed the study group. Among them, there were 62 cases in the control group (surgery+postoperative radiotherapy) and 81 cases in the study group (preoperative neo-adjuvant chemotherapy+surgery+postoperative radiotherapy). The treatment effect and adverse reactions of patients were compared between the two groups. RT-PCR was used to detect the expression levels of serum miR-182 of patients before and after treatment. The 1-year survival of patients in the two groups was recorded and compared by follow-up. RESULTS: The total effective rate of patients in the study group was significantly higher than that of patients in the control group (p<0.05). The incidence of adverse reactions of patients in the study group was significantly higher than in the control group (p<0.05). There was no significant difference in the prognostic 1-year survival rate between the two groups. After treatment, the expression of miR-182 was lower than before treatment and in the study group it was significantly lower than the control group (p<0.05). ROC curve analysis showed that the area under the curve of miR-182 in the predictive value of oral cancer was 0.756. When the cut-off value was less than 1.823, the optimal specificity was 70.18% and the sensitivity was 75.86%. CONCLUSION: Neo-adjuvant chemotherapy can significantly improve the therapeutic effect, but the incidence of adverse reactions increases. miR-182 may be involved in the occurrence and deterioration of oral cancer and is a good indicator for predicting the treatment efficacy of patients with oral cancer.

15.
Sci Total Environ ; : 143129, 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33121792

RESUMO

Aminoglycoside antibiotics (AAs) have been extensively applied in medical field and animal husbandry owing to desirable broad-spectrum antibacterial activity. Excessive AAs residues in the environment can be accumulated in human body through food chain and cause detrimental effect on human health. The establishment of highly specific, simple and sensitive detection methods for monitoring AAs residues is highly in demand. Aptasensor using aptamer as the biological recognition element is the efficient and promising sensing method for detection of AAs. In this review, we have made a summary of specific aptamers sequences against AAs. Subsequently, we provide a systematical and comprehensive overview of modern techniques in aptasensors for detection of AAs according to optical aptasensors as well as electrochemical aptasensors and further summarize their advantages and disadvantages to compare their applications. In addition, we present an overview of practical applications of aptasensors in sample detection of AAs. Moreover, the current challenges and future trends in this field are also included to reveal a promising perspective for developing novel aptasensors for AAs.

16.
ACS Omega ; 5(41): 26763-26775, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33111003

RESUMO

This paper involved conducting an experimental investigation on the effects of exhaust gas recirculation (EGR) and spark timing on the combustion, performance, and emission characteristics of a China-VI heavy-duty, natural gas engine fueled with high-methane content. The results showed that increasing the EGR rate extends the spark timing range and slows the combustion. This then increases ignition delay, prolongs combustion duration, and decreases heat release rate. Peak in-cylinder pressure (PCP) and indicated thermal efficiency (ITE) initially increase because of higher boost pressure with increasing EGR rate. However, as EGR rate increases further, PCP and ITE begin to decrease because of the deviation of combustion phasing. Lower in-cylinder temperature caused by higher EGR rate may cause nitrogen oxide (NOx) emissions to reduce significantly, while total hydrocarbon (THC) and carbon monoxide (CO) emissions increase, and THC emissions could increase exponentially at high EGR rates. In-cylinder pressure, temperature, and heat release rate increase with early spark timing, but the rate of increase is reduced at higher engine speeds. Early spark timing causes THC and CO emissions to increase at part-load conditions, whereas there is little change at full-load conditions. NOx emissions also increase with early spark timing because of the higher in-cylinder temperature.

17.
Int J Syst Evol Microbiol ; 70(10): 5330-5336, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32897847

RESUMO

A Gram-stain-negative, aerobic, non-motile and ovoid- to rod-shaped bacterial strain, designated GH877T, was isolated from a water sample of Gahai saline lake in Qaidam Basin,PR China. The isolate grew at 5-45 °C, pH 6.0-9.0 (optima, 37 °C and pH 7.5) and with 0.5-20 % (w/v) NaCl (optimum, 2.0 %). The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GH877T belonged to the genus Roseovarius, and had highest 16S rRNA gene sequence similarity of 97.7 % to Roseovarius pacificus 81-2T, followed by Roseovarius halotolerans HJ50T (97.5 %) and Roseovarius litoreus GSW-M15T (96.8 %). Genome sequencing revealed a genome size of 3 378 519 bp and a G+C content of 59.8 mol %. Up-to-date bacterial core gene set analysis indicated that strain GH877T represents one independent lineage with R. pacificus DSM29589T. The average nucleotide identity values of GH877T with R. pacificus 81-2T and R. halotolerans HJ50T are 80.7 and 77.3 %, respectively. In silico DNA-DNA hybridization values between strain GH877T and R. pacificus 81-2T and R. halotolerans HJ50T are 23.2 and 20.0 %, respectively. Q-10 was the predominant respiratory quinone and summed feature 8 (C18  :  1 ω7c and/or C18  :  1 ω6c) and C16  :  0 were the major cellular fatty acids. The polar lipids of strain GH877T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified phosphoglycolipids. Based on polyphasic taxonomic analysis, strain GH877T is proposed to represent a novel species of the genus Roseovarius, for which the name Roseovarius gahaiensis sp. nov. is proposed. (type strain GH877T=CGMCC 1.13971T=KCTC 72576T).


Assuntos
Lagos/microbiologia , Filogenia , Rhodobacteraceae/classificação , Águas Salinas , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
18.
Anal Methods ; 12(24): 3092-3097, 2020 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-32930168

RESUMO

Developing a sensitive, low-cost and general sensing platform for the analysis of a DNA biomarker and its mutation is important for early cancer screening. In our work, the tumor suppressor gene-p53 DNA was chosen as the model DNA biomarker due to its vital role in preventing oncogene cancer-inhibiting activity through mediating cellular proliferation and apoptosis. Compared with tumor biopsy, the quantification of p53 DNA and its mutation in biofluids (such as urine) is more convenient due to its simple operation and non-invasiveness. Herein, a label-free amplified fluorescence assay has been developed for p53 DNA in urine samples through the KFP polymerase-driven double strand displacement reactions and a magnetic nanoprobe. First, the ssDNA probe (RP) was designed with antisense sequences for p53 DNA and the Nb.BbvCI endonuclease recognition site. In the presence of p53 DNA, the formed dsDNA between RP and p53 DNA served as an engaging primer to initiate the first strand displacement reaction (SDA) under the action of KFP DNA polymerase and Nb.BbvCI, generating abundant short ssDNA (primer). Subsequently, the resulting primers will initiate the downstream SDA through the primer-hairpin DNA (HPa) binding, opening up, and extension of HPb and HPc under the action of KFP DNA polymerase. In the process of this final DNA polymerization reaction, the primer hybridized on HPa is released and goes on to initiate another round, forming plenty of duplex Y-shaped DNA. With the integration of SYBR Green I (SG I) into these duplex DNA, the amplified label-free fluorescence detection platform for p53 DNA can be achieved. Moreover, a biotin modified nanoprobe (bio-CP) was used to capture the superfluous HP. By performing the separation function, the binding of superfluous HP and SG could be avoided and a low background can be acquired. Benefiting from the abundant SG intercalation sites of Y-shaped DNA and low background signals, this method showed excellent sensitivity with a detection limit of 0.012 nM, and the p53 DNA in urine samples was evaluated, offering a powerful tool for biomedical research and clinical diagnosis.

19.
Bioorg Chem ; 104: 104205, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32916389

RESUMO

Zika virus (ZIKV) infection represents a significant threat to the global health system, and the search for efficient antivirals to ZIKV remains necessary and urgent. In this study, we extended the exploration of our previously discovered scaffold of 1H-pyrrolo[1,2-c]imidazol-1-one and revealed that two trans isomers of compounds 2 and 7 and one mixture with major trans isomer of compound 3 as novel tetrahydroquinoline-fused imidazolone derivatives are active against ZIKV infection but they are not virucidal. Western Blot and ELISA analyses of ZIKV NS5 and NS1 further demonstrate that compounds of (±)-2, (±)-3 and (±)-7 act as effective agents against ZIKV infection. We show that the N10's basicity is not the basic requirement for these compounds' antiviral activity in the current work. Importantly, tuning of some pharmacophores including substituents at arene can generate promising candidates for anti-ZIKV agents.

20.
Analyst ; 145(22): 7448, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-32966362

RESUMO

Correction for 'A facile deoxyuridine/biotin-modified molecular beacon for simultaneous detection of proteins and nucleic acids via a label-free and background-eliminated fluorescence assay' by Fei Yin, et al., Analyst, 2019, 144, 5504-5510, DOI: 10.1039/C9AN01016E.

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