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1.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2020-2025, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355555

RESUMO

To further study the mechanism of sprout tumble caused by drought,drought stress was simulating with 30% PEG 6000,physiological,and then the morphological changes of Pinellia ternata cells at different treatment time were detected. The results indicated that,along with the period of drought stress continued,the contents of chlorophyll and water potential were decreased,relative electrical conductivity,contents of soluble sugar and MDA increased. Sprout tumble of P. ternata first occurred on the fourth day during drought stress,large scale of sprout tumble appeared on the eighth day with about 73% of tumble rate. The nuclei exposed to drought stress for 2 days were flattened,lobed,invalidated or irregular in shape and significant showed the apoptotic morphological characteristics. Adenylate transferase( ANT) gene expressions were inhibited by drought,with the rapid increase of Caspase-3 enzyme activity,the cell death rate increased. All this proves that the essence of sprout tumble caused by drought is programmed cell death,which may be a self dormancy protection mechanism of P. ternata against adverse environment.


Assuntos
Apoptose , Secas , Pinellia/citologia , Estresse Fisiológico
2.
Zhongguo Zhong Yao Za Zhi ; 43(19): 3855-3861, 2018 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-30453709

RESUMO

To explore the effects of shading and the expression of key enzyme genes on the synthesis and accumulation of Panax japonicus var. major saponins, different shading treatments (0%, 30%,50%) of potted P. japonicus var. major were used as test materials, the expression of three key enzyme genes(CAS,DS,ß-AS) of leaves and rhizomes in different growth periods of P. japonicus var. major was determined by real-time quantitative PCR, the content of total saponins was determined by ultraviolet spectrophotometry. The results indicated that, in flowering stage, CAS,DS,ß-AS were highly expressed in the aerial parts of P. japonicus var. major, 30% shading treatment significantly inhibited the expression of CAS in leaves and promoted the expression of DS and ß-AS in stems, leaves and flowers, it was speculated that the main part of saponin synthesis was leaf in this stage. Both the expression levels of DS and ß-AS and changes in the content of total saponins in leaves showed a tendency of low-high-low throughout the growth cycle, correlation coefficient analysis showed that there was a positive correlation between them. Compared with control, the expression levels of DS and ß-AS and the content of total saponins were greatly enhanced under shading treatment, 30% shading treatment significantly promoted the accumulation of total saponins. Therefore, it is suggested that 30% shading treatment should be applied to the artificial cultivation of P. japonicus var. major, which is beneficial to the accumulation and quality improvement of saponins.


Assuntos
Luz , Panax/enzimologia , Panax/efeitos da radiação , Saponinas/análise , Regulação da Expressão Gênica de Plantas , Panax/genética , Folhas de Planta/enzimologia , Folhas de Planta/genética , Rizoma/enzimologia , Rizoma/genética
3.
Zhongguo Zhong Yao Za Zhi ; 43(2): 353-362, 2018 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29552855

RESUMO

To explore the flavor and meridian tropism classification of Callianthemum taipaicum by principal components analysis(PCA) and partial least square analysis(PLS). Meanwhile,to establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-ESI-MS) method for the simultaneous determination of 55 active components from 13 kinds of Ranunculaceae of Chinese traditional herbs. Samples were separated on HPLC system by Agilent 5 TC-C18(2)(4.6 mm×250 mm,5 µm)column and eluted with acetonitrile and 0.1% formic acid at the flow rate of 0.6 mL·min⁻¹. The data were performed by HPLC-ESI-MS with multiple reaction monitoring(MRM)scanning mode under positive and negative ion modes and quantified by external standards. The data from 13 Ranunculaceae herbs were analyzed by the PLS-tree and cooman's prediction of PCA and PLS to evaluate the similarities and differences of C. taipaicum in flavor and meridian tropism. The results showed that calibration curves of 55 components all showed good linearity, r>0.99,with good precision, repeatability and stability. After compared to other 12 herbs,PCA and PLS results revealed that the C. taipaicum belonged to lung and bladder meridians while its flavor attributive to pungent,warm in nature. In conclusion,the analysis approach of chemometric calculation combined with multi-components quantification is suitable for the classification of meridian tropism and flavor of Chinese traditional medicine,which can be used for alternative research of rare herbs.


Assuntos
Medicamentos de Ervas Chinesas/química , Meridianos , Plantas Medicinais/química , Ranunculaceae/química , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Compostos Fitoquímicos/análise , Espectrometria de Massas em Tandem
4.
Nan Fang Yi Ke Da Xue Xue Bao ; 35(1): 12-6, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-25613602

RESUMO

OBJECTIVE: To investigate the effect of chloroquine on airway hyperresponsiveness in asthmatic mice and explore the possible mechanism. METHODS: Balb/c mouse models of asthma established using OVA received intraperitoneal injections of chloroquine, dexamethasone, or both prior to OVA challenge. Within 24 h after the final challenge, airway hyper- responsiveness (AHR) of the mice was assessed, and the total cell count and the counts of different cell populations in the bronchoalveolar lavage fluid (BALF) were determined under light microscopy. The severity of lung inflammation was evaluated using HE staining, and the concentrations of IL-6 and PGF2α in the BALF were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: Chloroquine pretreatment significantly decreased AHR (P<0.001) in the asthmatic mice and reduced the total cell count (P<0.01), eosinophils (P<0.001), neutrophils (P<0.01), and PGF2α levels in the BALF. Chloroquine combined with low-dose dexamethasone significantly lessened inflammations around the bronchioles (P<0.05) and blood vessels (P<0.01) in the lung tissue, and obviously lowered IL-6 (P<0.05) and PGF2α (P<0.001) in the BALF in the asthmatic mice. CONCLUSION: Chloroquine can inhibit AHR in asthmatic mice and produce better anti-inflammatory effect when combined with dexamethasone for treatment of neutrophilic asthma.


Assuntos
Asma/tratamento farmacológico , Cloroquina/farmacologia , Animais , Asma/induzido quimicamente , Líquido da Lavagem Broncoalveolar/citologia , Dexametasona/farmacologia , Dinoprosta/metabolismo , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Eosinófilos/citologia , Inflamação/patologia , Interleucina-6/metabolismo , Contagem de Leucócitos , Pulmão/patologia , Camundongos , Camundongos Endogâmicos BALB C , Neutrófilos/citologia
5.
Sci Rep ; 4: 3728, 2014 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-24430984

RESUMO

A large and sudden increase in radiocarbon ((14)C) around AD 773 are documented in coral skeletons from the South China Sea. The (14)C increased by ~ 15‰ during winter, and remain elevated for more than 4 months, then increased and dropped down within two months, forming a spike of 45‰ high in late spring, followed by two smaller spikes. The (14)C anomalies coincide with an historic comet collision with the Earth's atmosphere on 17 January AD 773. Comas are known to have percent-levels of nitrogen by weight, and are exposed to cosmic radiation in space. Hence they may be expected to contain highly elevated (14)C/(12)C ratios, as compared to the Earth's atmosphere. The significant input of (14)C by comets may have contributed to the fluctuation of (14)C in the atmosphere throughout the Earth's history, which should be considered carefully to better constrain the cosmic ray fluctuation.


Assuntos
Antozoários/química , Radioisótopos de Carbono/química , Radiação Cósmica , Animais , China
6.
Yao Xue Xue Bao ; 46(6): 738-41, 2011 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-21882538

RESUMO

This study is to investigate the effects of puerarin on the proliferation and differentiation of umbilical cord mesenchymal stem cells (MSCs) into osteoblasts. Umbilical cord MSCs were cultured by tissue adherence and the third passage of cells was used in the experiment. The effect of puerarin on proliferation of umbilical cord MSCs was measured with MTT. The effects of puerarin on umbilical cord MSCs were evaluated by ALP immunohistochemisty and von kossa staining. The OD value decreased with the increase of puerarin concentration. On 7th day, ALP expression of puerarin group was higher than that of control group. On 14th day, ALP staining showed that the positive rate of puerarin group was higher than that of control group. Von kossa staining showed the quantity of calcium nodules was higher in puerarin group than that of control group. Puerarin can promote the umbilical cord MSCs to differentiate into osteoblasts and has an effect on the proliferation of umbilical cord MSCs.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Isoflavonas/farmacologia , Células-Tronco Mesenquimais/citologia , Osteoblastos/citologia , Células Cultivadas , Humanos , Isoflavonas/isolamento & purificação , Osteogênese/efeitos dos fármacos , Plantas Medicinais/química , Pueraria/química , Cordão Umbilical/citologia
7.
Phytopathology ; 100(5): 468-73, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20373968

RESUMO

Tan spot, caused by Pyrenophora tritici-repentis, is an economically important foliar disease of wheat worldwide. Eight races of the pathogen have been characterized on the basis of their ability to cause necrosis or chlorosis in a set of differential wheat lines. Race 1 produces two host-selective toxins, Ptr ToxA and Ptr ToxC, that induce necrosis and chlorosis, respectively, on leaves of sensitive wheat genotypes. A population of recombinant inbred lines was developed from a cross between Chinese landrace Wangshuibai (resistant) and Chinese breeding line Ning7840 (highly susceptible) to identify chromosome regions harboring quantitative trait loci (QTL) or genes for tan spot resistance. Plants were inoculated at the four-leaf stage in a greenhouse and percent leaf area diseased was scored 7 days after inoculation. Two QTL for resistance to race 1 were mapped to the short arms of chromosomes 1A and 2B in the population. The QTL on 1AS, designated as QTs.ksu-1AS, showed a major effect and accounted for 39% of the phenotypic variation; the QTL on 2BS, designated as QTs.ksu-2BS, explained 4% of the phenotypic variation for resistance. A toxin infiltration experiment demonstrated that both parents were insensitive to Ptr ToxA, suggesting that the population was most likely segregating for reaction to chlorosis, not necrosis. The markers closely linked to the QTL should be useful for marker-assisted selection in wheat-breeding programs.


Assuntos
Ascomicetos/fisiologia , Predisposição Genética para Doença , Locos de Características Quantitativas , Triticum/genética , Triticum/microbiologia , China , Ligação Genética , Marcadores Genéticos , Doenças das Plantas/genética , Doenças das Plantas/microbiologia
8.
Acta Crystallogr Sect E Struct Rep Online ; 66(Pt 12): m1574, 2010 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-21589260

RESUMO

In the title compound, [Ni(C(14)H(8)O(5))(C(10)H(8)N(2))](n), the Ni(II) atom is six-coordinated in a slightly distorted octa-hedral geometry by four O atoms from two chelating carboxyl-ate groups of symmetry-related 2,4'-oxydibenzoate anions and by two N atoms from a 2,2'-bipyridine ligand. The Ni(II) atoms are bridged by the 2,4'-oxydibenzoate anions, resulting in the formation of helical chains parallel to [010] with a repeating unit of 15.039 (2) Å.

9.
Zhonghua Fu Chan Ke Za Zhi ; 44(11): 809-11, 2009 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-20079029

RESUMO

OBJECTIVE: To investigate the expression and clinical significance of platelet-derived growth factor A (PDGF-A) on placenta tissue from pre-eclampsia. METHODS: The expression of PDGF-A in the placenta of 38 pre-eclampsia patients and 22 normal pregnant women at third trimester was detected by immunohistochemistry method. RESULTS: (1) PDGF-A was mainly expressed in the cytomembrane and cytoplasm of cytotrophoblasts and the endothelial cell of capillary in placenta. (2) The rates of PDGF-A expression of cytotrophoblasts were 63% (24/38) in pre-eclampsia group and 32% (7/22) in normal pregnancy group, which exhibited significant difference (P < 0.05). (3) The rates of PDGF-A expression of endothelial cell were 68% (26/38) in pre-eclampsia group and 27% (6/22) in normal pregnancy group, which also showed significant difference (P < 0.01). (4) The rates of PDGF-A expression of cytotrophoblasts were 39% (7/18) in mild pre-eclampsia patients and 85% (17/20) in severe pre-eclampsia, which reached statistical difference (P < 0.01). CONCLUSION: The increasing expression of PDGF-A in cytotrophoblast and endothelial cell in placenta might confer the occurrence and progression of pre-eclampsia.


Assuntos
Placenta , Pré-Eclâmpsia , Feminino , Humanos , Imuno-Histoquímica , Placenta/metabolismo , Pré-Eclâmpsia/metabolismo , Gravidez , Terceiro Trimestre da Gravidez , Trofoblastos/metabolismo
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