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1.
World J Clin Cases ; 9(27): 8249-8259, 2021 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-34621888

RESUMO

BACKGROUND: Granulomatous lobular mastitis (GLM) is a type of benign chronic inflammatory disease that poses therapeutic challenges to healthcare providers. The diagnosis of GLM relies on tissue biopsy, and incorrect treatment may lead to delayed diagnosis, considerable aesthetic damage, and even mastectomy. CASE SUMMARY: We report the case of a 37-year-old Chinese woman who was lactating and had GLM in both breasts. At the time of treatment, the right breast had a mass of approximately 15 cm × 11 cm, which was hard and had poor mobility. Multiple skin ulcerations and pus spills were also observed on the surface of the breast. The left breast had a mass of about 13 cm × 9 cm, which was hard and had poor mobility. CONCLUSION: Herein, we report a case of bilateral GLM in a lactating woman that was successfully treated with traditional Chinese medicine (TCM), without the requirement for surgery or other treatments. Therefore, TCM may have advantages in the nonsurgical treatment of GLM.

2.
Head Neck ; 43(11): 3386-3392, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34423875

RESUMO

BACKGROUND: Whether patients with medullary thyroid carcinoma (MTC) who have unresectable synchronous distant metastases should undergo primary surgical resection (PTR) remains controversial. This study aimed to identify predictive factors associated with the survival of such patients. METHODS: We conducted a retrospective study of patients with MTC who were registered in the Surveillance, Epidemiology, and End Results registry. The overall and cancer-specific mortality rates were assessed using risk-adjusted Cox proportional hazards regression modeling and stratified propensity score matching. RESULTS: One hundred and eight matched patients were assessed. Patients in the PTR group had lower overall mortality than did those in the non-PTR group. The 1-, 3-, and 5-year overall and cancer-specific survival rates in the PTR group were significantly higher. CONCLUSIONS: PTR appears to be the most appropriate intervention for patients with good performance status. Such patients are likely to benefit from surgery and to experience long-term stable disease.


Assuntos
Carcinoma Neuroendócrino , Neoplasias da Glândula Tireoide , Carcinoma Neuroendócrino/cirurgia , Humanos , Pontuação de Propensão , Estudos Retrospectivos , Taxa de Sobrevida , Neoplasias da Glândula Tireoide/cirurgia
3.
mSystems ; 6(3): e0119020, 2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34061577

RESUMO

Zika virus (ZIKV; Flaviviridae) is a devastating virus transmitted to humans by the mosquito Aedes aegypti. The interaction of the virus with the mosquito vector is poorly known. The double-stranded RNA (dsRNA)-mediated interruption or activation of immunity-related genes in the Toll, IMD, JAK-STAT, and short interfering RNA (siRNA) pathways did not affect ZIKV infection in A. aegypti. Transcriptome-based analysis indicated that most immunity-related genes were upregulated in response to ZIKV infection, including leucine-rich immune protein (LRIM) genes. Further, there was a significant increment in the ZIKV load in LRIM9-, LRIM10A-, and LIRM10B-silenced A. aegypti, suggesting their function in modulating viral infection. Further, gene function enrichment analysis revealed that viral infection increased global ribosomal activity. Silencing of RpL23 and RpL27, two ribosomal large subunit genes, increased mosquito resistance to ZIKV infection. In vitro fat body culture assay revealed that the expression of RpL23 and RpL27 was responsive to the Juvenile hormone (JH) signaling pathway. These two genes were transcriptionally regulated by JH and its receptor methoprene-tolerant (Met) complex. Silencing of Met also inhibited ZIKV infection in A. aegypti. This suggests that ZIKV enhances ribosomal activity through JH regulation to promote infection in mosquitoes. Together, these data reveal A. aegypti immune responses to ZIKV and suggest a control strategy that reduces ZIKV transmission by modulating host factors. IMPORTANCE Most flaviviruses are transmitted between hosts by arthropod vectors such as mosquitoes. Since therapeutics or vaccines are lacking for most mosquito-borne diseases, reducing the mosquito vector competence is an effective way to decrease disease burden. We used high-throughput sequencing technology to study the interaction between mosquito Aedes aegypti and ZIKV. Leucine-rich immune protein (LRIM) genes were involved in the defense in response to viral infection. In addition, RNA interference (RNAi) silencing of RpL23 and RpL27, two JH-regulated ribosomal large subunit genes, suppressed ZIKV infection in A. aegypti. These results suggest a novel control strategy that could block the transmission of ZIKV.

4.
Ying Yong Sheng Tai Xue Bao ; 32(4): 1279-1288, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-33899396

RESUMO

Analyzing the effects of nutrient addition on the functional traits of desert plants is important for revealing the responses of desert plant species to environmental changes. In this study, we examined the responses of whole plant, root, stem, leaf and fruit traits of Lycium ruthenicum to the addition of N and P, with an experiment with three (low, medium and high) N and P addition levels and three N/P ratios (5:1, 15:1 and 45:1). The results showed that functional traits of L. ruthenicum had divergent responses to NP addition level and N/P ratio. With the increases of NP addition level, the biomass and specific leaf area were increased, while the root-shoot ratio, leaf dry matter content, root tissue density and specific root length were decreased. Belowground biomass, specific root length and net photosynthetic rate increased with the increases of N/P ratio. The coefficient of variation of 17 functional traits was 7.3%-69.1%. The biomass, root-shoot ratio and speci-fic root length were sensitive traits to NP [plastic index (PI)>0.5], with greater variability (49.4%-69.1%), whereas the leaf length-width ratio, leaf thickness, leaf tissue density, and leaf stem dry matter content were conservative traits (PI<0.20). The results of principal component analysis (PCA) showed that the position of L. ruthenicum in the multivariate feature space exhibited lateral migration with the changes of NP addition levels, with a tendency of higher aboveground and belowground biomass and a lower root-shoot ratio. Leaf tissue density was negatively related to leaf thickness and specific leaf area. Leaf dry matter content was negatively correlated with leaf thickness and specific leaf area but positively associated with leaf tissue density. Biomass had a positive correlation with specific leaf area and a negative relation to specific root length. The results of stepwise regression analysis showed that specific root length, specific leaf area and leaf net photosynthetic rate were major functional traits affecting the biomass of L. ruthenicum. L. ruthenicum adapted to the fluctuations of soil nutrient environment through changing resource utilization strategy, altering root carbon allocation, and also the trade-off and covariance among traits and inconsistent response.


Assuntos
Lycium , Biomassa , Fenótipo , Folhas de Planta , Solo
5.
Sci Rep ; 11(1): 8072, 2021 04 13.
Artigo em Inglês | MEDLINE | ID: mdl-33850252

RESUMO

The purpose of this study was to explore the feasibility of eustachian tube optical coherence tomography (ET-OCT) for imaging the pharyngeal region of the eustachian tube (ET). Ten subjects with ear complaints underwent ET-OCT guided by nasal endoscopy, and ET-OCT examination was performed on both sides of each subject's ETs. The process and resulting images were analysed. Ten subjects ranging from 21 to 73 years old (45 ± 14.77) were enrolled in this study. Eighteen ET-OCT imaging examinations were completed. The mean duration of each examination was 2.80 ± 1.62 min (ranging from 2 to 7 min). There were no adverse events or complications. In some subjects, the ET-OCT images clearly presented the microstructures of the ET wall, including the lumen, mucosa, submucosa, cartilage and plica. However, in some subjects, it showed different characteristics, such as an unclear hierarchy and secretions in the lumen. ET-OCT may help to distinguish the structural composition of the ET and elucidate related pathophysiological mechanisms. It is a valuable imaging tool suited for the ET, with potential diagnostic value in determining the morphology of the lumen, intraluminal mucosa and submucosal tissue in the pharyngeal region of the ET.

6.
BMC Pediatr ; 21(1): 126, 2021 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-33722205

RESUMO

BACKGROUND: Phenylketonuria (PKU) is a genetic metabolic disorder in which patients have no ability to convert phenylalanine to tyrosine. Several autoimmune diseases have been reported to combine with PKU, co-existent of PKU and Juvenile Idiopathic Arthritis (JIA) has not been presented. CASE PRESENTATION: The girl was diagnosed with PKU at the age of 1 month confirmed by molecular data. At the age of 3.5 years, she presented with pain and swelling of her right ankle, right knee, and right hip joint. After a serial of examinations, she was diagnosed with JIA and treated with a nonsteroidal anti-inflammatory drug. CONCLUSIONS: We report a rare case of a 4-year-old girl with PKU and JIA, which supports a possible interaction between PKU and JIA. Long-term metabolic disturbance may increase the susceptibility to JIA. Further chronic inflammation could alter the metabolism of tryptophan and tyrosine to increase blood Phe concentration. In addition, corticosteroid and methotrexate therapy for JIA may increase blood Phe concentration.


Assuntos
Artrite Juvenil , Fenilcetonúrias , Corticosteroides/uso terapêutico , Anti-Inflamatórios não Esteroides/uso terapêutico , Artrite Juvenil/complicações , Artrite Juvenil/diagnóstico , Artrite Juvenil/tratamento farmacológico , Pré-Escolar , Feminino , Humanos , Fenilalanina , Fenilcetonúrias/complicações , Fenilcetonúrias/diagnóstico
7.
J Integr Neurosci ; 19(2): 249-257, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32706189

RESUMO

The gene PSEN2 encodes presenilin-2, a subunit of γ-secretase. Mutations in PSEN2 are not only related to Alzheimer's disease but are also involved in other diseases. The Chinese tree shrew (Tupaia belangeri chinensis) is a potential animal model for Alzheimer's disease, although little is known about its cDNA sequence, protein structure, and PSEN2 expression. To better understand PSEN2 in the tree shrew, we cloned this gene by rapid amplification of cDNA ends technology. Hence, we analyzed the sequence and molecular characteristics of PSEN2 mRNA, predicted its spatial structure, and analyzed its expression profiles. We found that tree shrew PSEN2 is 1539 base pairs in length and encodes 330 amino acids. It is homologous and genetically similar to humans (97.64% identity). The protein structure of tree shrew PSEN2 indicated similarities to human PSEN2, both being comprised of numerous transmembrane helices. However, tree shrew PSEN2 possesses seven α-helices, and thus lacks three compared with human PSEN2. Tree shrew PSEN2 mRNAs were ubiquitously detected in all tissues, with a tissue- and temporal-specific pattern. These results pave the way towards the function of tree shrew PSEN2, which will give insights into the mechanisms leading to neurodegenerative and other diseases in humans.


Assuntos
Presenilina-2/genética , Transcriptoma/genética , Tupaia/genética , Animais , DNA Complementar , Modelos Animais de Doenças , RNA Mensageiro
8.
Biosci Rep ; 39(6)2019 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-31092700

RESUMO

BACKGROUND: Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4 intronic transcript 1 (SPRY4-IT1) predicates poor prognosis and promotes tumor progress in cervical cancer (CC). However, the underlying mechanism of SPRY4-IT1 in CC remains unknown. The aim of the present study is to evaluate the function and mechanism of SPRY4-IT1 in CC. METHODS: SPRY4-IT1 was detected by quantitative PCR. Wound-healing assay and Transwell assay were performed to detect cell migration and invasion, respectively. Western blotting assays were used to analyze the protein expression of E-cadherin, N-cadherin and vimentin. Tumor xenografts experiments were performed to detect the effect of SPRY4-IT1 in vivo. Dual luciferase reporter assay was used to investigate potential molecular mechanism of SPRY4-IT1 in CC cells. RESULTS: SPRY4-IT1 was up-regulated in CC cell lines. Knockdown of SPRY4-IT1 significantly inhibited CC cells migration and invasion in vitro and in vivo Moreover, knockdown of SPRY4-IT1 significantly suppressed the epithelial-mesenchymal transition (EMT) of CC by increased E-cadherin expression and decreased the N-cadherin and vimentin expression. Mechanically, SPRY4-IT1 could directly bind to miR-101-3p and effectively act as a competing endogenous RNA (ceRNA) for miR-101-3p to regulate the expression of the target gene ZEB1Conclusions: Our findings indicate that the SPYR4-IT1/miR-101-3p/ZEB1 axis contributes to CC migration and invasion, which may provide novel insights into the function of lncRNA-driven tumorigenesis of CC.


Assuntos
Transição Epitelial-Mesenquimal/genética , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , RNA Longo não Codificante/genética , Neoplasias do Colo do Útero/genética , Homeobox 1 de Ligação a E-box em Dedo de Zinco/genética , Animais , Caderinas/genética , Caderinas/metabolismo , Linhagem Celular Tumoral , Movimento Celular/genética , Feminino , Células HeLa , Humanos , Camundongos Endogâmicos BALB C , Camundongos Nus , Invasividade Neoplásica , Interferência de RNA , Transdução de Sinais/genética , Carga Tumoral/genética , Neoplasias do Colo do Útero/metabolismo , Neoplasias do Colo do Útero/patologia , Ensaios Antitumorais Modelo de Xenoenxerto/métodos , Homeobox 1 de Ligação a E-box em Dedo de Zinco/metabolismo
9.
Emerg Microbes Infect ; 8(1): 232-241, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30866776

RESUMO

Zika virus (ZIKV) is a mosquito-borne flavivirus that caused the public health emergency. Recently, we have proved a novel small animal tree shrew was susceptive to ZIKV infection and presented the most common rash symptoms as ZIKV patients. Here we further cultured the primary cells from different tissues of this animal to determine the tissue tropism of ZIKV infection in vitro. The results showed that the primary cells from tree shrew kidney, lung, liver, skin and aorta were permissive to ZIKV infection and could support viral replication by the detection of viral specific RNA intra- and extra-cells. In comparing, the skin fibroblast and vascular endothelial cells were highly permissive to ZIKV infection with high releasing of active virus particles in supernatants proved by its infectivity in established neonatal mouse model. The expressions of ZIKV envelop and nonstructural protein-1, and the effects and strong immune response of primary tree shrew cells were also detected followed by ZIKV infection. These findings provide powerful in vitro cell-level evidence to support tree shrew as animal model of ZIKV infection and may help to explain the rash manifestations in vivo.


Assuntos
Modelos Animais de Doenças , Tupaiidae/virologia , Infecção por Zika virus/virologia , Zika virus/patogenicidade , Animais , Aorta/citologia , Aorta/virologia , Células Cultivadas , Chlorocebus aethiops , Células HEK293 , Células Endoteliais da Veia Umbilical Humana , Humanos , Rim/citologia , Rim/virologia , Fígado/citologia , Fígado/virologia , Pulmão/citologia , Pulmão/virologia , Pele/citologia , Pele/virologia , Células Vero , Replicação Viral
10.
Vet Microbiol ; 213: 5-14, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29292004

RESUMO

H9N2 subtype low pathogenic avian influenza virus (LPAIV) is distributed worldwide and causes great economic losses in the poultry industry, especially when complicated with other bacterial infections. Tissue damages caused by virus infection provide an opportunity for bacteria invasion, but this mechanism is not sufficient for low pathogenic strains. Moreover, although H9N2 virus infection was demonstrated to promote bacterial infection in several studies, its mechanism remained unclear. In this study, infection experiments in vivo and in vitro demonstrated that the adhesion of Escherichia coli (E. coli) to host cells significantly increased after H9N2 virus infection, and this increase was not caused by pathological damages. Subsequently, we constructed a late chicken embryo infection model and used proteomics techniques to analyze the expression of proteins associated with bacterial adhesion after H9N2 virus infection. A total of 279 significantly differential expressed proteins were detected through isobaric tags for relative and absolute quantitation (iTRAQ) coupled with nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) analysis. The results of Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that differentially expressed proteins were enriched in host innate immunity; cell proliferation, differentiation, and apoptosis; and pathogenicity-related signaling pathways. Finally, we screened out several proteins, such as TGF-ß1, integrins, cortactin, E-cadherin, vinculin, and fibromodulin, which were probably associated with bacterial adhesion. The study analyzed the mechanism of secondary bacterial infection induced by H9N2 virus infection from a novel perspective, which provided theoretical and data support for investigating the synergistic infection mechanism between the H9N2 virus and bacteria.


Assuntos
Aderência Bacteriana , Escherichia coli/fisiologia , Vírus da Influenza A Subtipo H9N2/fisiologia , Influenza Aviária/virologia , Proteômica , Animais , Apoptose , Diferenciação Celular , Proliferação de Células , Embrião de Galinha , Galinhas , Coinfecção , Imunidade Inata , Pulmão/embriologia , Pulmão/microbiologia , Sistema Respiratório/microbiologia
11.
Animal Model Exp Med ; 1(2): 152-161, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30891560

RESUMO

Backgroud: Current understanding of injury and regeneration of islet ß-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of ß-cells in tree shrews. Methods: In this study, we examined the changes in islet cell composition and regeneration of ß-cells after streptozotocin (STZ) treatment in tree shrews compared with Sprague-Dawley rats. Injury and regeneration of islet ß-cells were observed using hematoxylin and eosin (HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and PDX-1. Results: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet ß-cells was very pronounced in rats, and mainly involved regeneration of centro-acinar cells and transformation of extra-islet ductal cells. In tree shrews, the regeneration of islet ß-cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro-acinar cells was observed. Conclusion: The results suggest that the repair mechanism of islet ß-cells in tree shrews is similar to that of humans.

12.
Medicine (Baltimore) ; 96(52): e9511, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29384955

RESUMO

RATIONALE: Cavernous sinus-carotid fistula (CCF) is a rare complication of craniofacial injuries in patients receiving oral and maxillofacial surgical care. PATIENT CONCERNS: A retrospective review of 15 patients with CCF records was conducted. In addition, we present a rare case of a 32-year-old Chinese woman with CCF receiving oral and maxillofacial surgical care. DIAGNOSES: Digital subtraction angiography (DSA) confirmed a diagnosis of CCF. INTERVENTIONS: Endovascular surgery was performed via the femoral artery under general anesthesia without any complications. OUTCOMES: The postoperative course was uneventful, and the subjective and objective ophthalmic symptoms had resolved. LESSONS: The symptoms of CCF may be delayed for several days or weeks. Thus, maxillofacial surgeons should be aware of this and avoid the untimely repair of facial fractures with potentially disastrous consequences. Interventional neuroradiologic approaches that involve the use of a detachable balloon have made it possible to care for patients with CCF without any complications.


Assuntos
Fístula Carotidocavernosa/etiologia , Traumatismos Craniocerebrais/complicações , Adulto , Angiografia Digital , Fístula Carotidocavernosa/cirurgia , Traumatismos Craniocerebrais/cirurgia , Traumatismos Faciais/complicações , Traumatismos Faciais/cirurgia , Feminino , Humanos , Tomografia Computadorizada por Raios X
13.
PLoS One ; 11(10): e0164840, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27760217

RESUMO

INTRODUCTION: Although anaplastic thyroid carcinoma (ATC) is rare, it is one of the most aggressive human cancers. The optimal multimodal therapy policy of ATC is still debated, and a standardized treatment strategy remains to be established. This study aimed to evaluate the management aspect and prognosis of ATC. MATERIALS AND METHODS: The data were analyzed retrospectively for 50 patients with ATC to evaluate the clinical characters, management and factors influencing survival. Survival analysis was performed by Kaplan-Merier method and log-rank test, and multivariate analysis was performed using Cox proportional hazard model. RESULTS: The 1-year and 2-year overall survival rates (OS) were 48.0% and 26.0% respectively in all patients, with the 2-year OS of 40.0% and 31.0% and 6.3% for stage IVA, IVB and IVC respectively (P <0.05). In stage IVA and IVB patients, combined surgery with radiotherapy improved overall survival, and the 2-year OS were 50.0% and 35.7% respectively in the group with combined surgery with radiotherapy and the group with surgery with only (P <0.05). Postoperative radiotherapy improved local control rate in stage IVA and IVB patients (P <0.05). However, surgery, radiotherapy or chemotherapy could not improve the survival of stage IVC patients. Multivariate analysis showed that distant metastases, surgery, radiotherapy and tumor residue could predict the prognosis. CONCLUSION: Combined surgery and radiotherapy could improve overall survival in stage IVA and IVB patients. Patients with ATC have a bad prognosis. Distant metastases, surgery, radiotherapy and tumor residue are the most important factors affecting the prognosis.


Assuntos
Carcinoma Anaplásico da Tireoide/diagnóstico , Carcinoma Anaplásico da Tireoide/terapia , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos , Taxa de Sobrevida , Carcinoma Anaplásico da Tireoide/patologia , Neoplasias da Glândula Tireoide/patologia
14.
Mol Clin Oncol ; 5(1): 129-131, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27330783

RESUMO

Hashimoto's thyroiditis with thyroid cancer in childhood is not as common in the adult population. Hashimoto's thyroiditis is an autoimmune disease associated with autoantibodies, and the association between Hashimoto's thyroiditis and papillary carcinoma of the thyroid remains controversial. The present study reported a 15-year-old adolescent girl with the diagnosis of Hashimoto's thyroiditis with thyroid cancer. With the complexity of the clinical manifestations of Hashimoto's thyroiditis, it can be expressed as not only hyperthyroidism or hypothyroidism, but also normal thyroid function. The long-term treatment, and for children with thyroid cancer, early diagnosis is particularly difficult. In the present case, the diagnosis of Hashimoto's thyroiditis is primarily based on clinical manifestations, anti-thyroglobulin antibody and anti-thyroid microsomal antibody. The only diagnostic imaging ultrasound was negative. The present study discussed the possible reason and the identification of this unique case of Hashimoto's thyroiditis with thyroid cancer.

15.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 38(1): 42-8, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26956855

RESUMO

OBJECTIVE: To observe the characteristic morphological changes of corneal endothelial dysfunction induced by phacoemulcification in rhesus monkey models under confocal microscope. METHODS: The corneal endothelial dysfunction models were established by phacoemulcification power on the central corneal of 7 to 9 mm diameter in the right eyes of 4 rhesus monkeys (the modeling group). The left eyes of 4 rhesus monkeys were set as blank control group. The structural changes in different corneal layers were evaluated by slit lamp microscope and in vivo confocal microscope before surgery and 1, 2, 3, and 4 weeks after surgery. SPSS 19.0 software was applied to analyze data. Paired-t test was used to compare the number of nerve plexus in Bowman's layer and corneal endothelial cell density. Analysis of variance (ANOVA) was used to analyze corneal thickness. RESULTS: After phacoemulcification, the changes of cornea occurred gradually in the endothelial layer, stroma, Bowman's membrane, and basal epithelial layer. In the early stage, the interspace of corneal endothelial cells enlarged and few activated stromal cells were detected in the stroma. The cell morphology of stroma altered. The thickness of stroma increased. Two weeks after surgery, the nerve plexus in Bowman's layer decreased and edema of stroma and endothelial layer increased. Three weeks after surgery, the interspace of basal epithelial cells increased with a few Langerhans' cells infiltration and edema of stroma and endothelial layer increased. Four weeks after the surgery, a large amount of Langerhans' cells presented in basal epithelial layer. Only a few nerve lexus could be seen in Bowman's layer. The stroma and endothelial cells had severe edema. A large number of activated stromal cells could be found in stromal layer. Two weeks after the surgery, the number of nerve plexus in Bowman's layer (t=6.9192, P=0.002) and corneal endothelial cell density (t=7.8936, P<0.0001) in the modeling group were significantly lower than that in control group. Compared with corneal thickness in control group, it was significantly larger in the modeling group at 1 (t=28.31, P<0.0001), 2 (t=63.56, P<0.0001), 3 (t=123.22, P<0.0001), and 4 weeks (t=180.80, P<0.0001) after the surgery. CONCLUSIONS: The changes in corneal endothelial dysfunction induced by phacoemulcification in rhesus monkey models can be clearly shown under in vivo confocal microscope. Gradual increase of endothelial cells interspace, activated stromal cells, increase of Langerhans' cells, and decrease of plexus in Bowman's layer are the main changes.


Assuntos
Doenças da Córnea , Células Endoteliais , Animais , Células de Langerhans , Macaca mulatta , Microscopia Confocal
16.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2561-2565, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905586

RESUMO

Herbarium specimens are the basis for the plant classification and indispensable media in teaching, scientific research and resources investigation. They have also played an important role in identifying and producing traditional Chinese medicine. High-quality herbarium specimens shall meet high requirements for integrity, smoothness, color and fabricating efficiency. Therefore, we designed a rapid setting and drying device for herbarium specimens, which could make the herbarium specimens smooth, colorful and not easy to mildew. In this paper, we pointed out the deficiency of traditional methods in making herbarium specimens, and introduced the structure and working principle of the device. Besides, we also discussed the effect of the device in setting and drying herbarium specimens and its application in the fourth national survey of the Chinese material medica resources (CMMR) in Anhui province. As a result, the device provides new ideas for producing herbarium specimens, with a reasonable design, good uniformity, high efficiency, safety and portability, and so is worthy of promotion and application in the national survey of CMMR.


Assuntos
Dessecação/instrumentação , Plantas Medicinais , Manejo de Espécimes/métodos , Medicamentos de Ervas Chinesas , Materia Medica , Medicina Tradicional Chinesa , Manejo de Espécimes/instrumentação , Inquéritos e Questionários
17.
Zhongguo Zhong Yao Za Zhi ; 41(15): 2814-2818, 2016 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-28914021

RESUMO

Xiangsha Yangwei pill was selected as a model drug in this research, and time domain reflectometry (TDR) was used to determine the water content in the pill. The effects of five factors including the number of pill layers, pill packing density, atmospheric moisture, ambient temperature and the ratio of pill formula were investigated on water content. The results showed that the number of pill layers and ambient temperature had significant effects on water content of pills, while the pill packing density, atmospheric moisture and pill formula ratio had little effect on the determination of water content in pills. The reflection value was stable when 6 layers of pills were used. Under the condition of 25 ℃ and 45% relative humidity, the water content of pills ranged from 4.01% to 22.38%, showing good linear relationship between water content and reflection value, and the model equation was as follows: Y=0.279X-21.670 (R²=0.997 0). Verification experiment was used to explain the feasibility of this prediction model. The precision of the method complied with the methodology standard. It is concluded that TDR can be used in determination of water content in Xiangsha Yangwei pills. Additionally, TDR, as a new way to quickly and efficiently determine the water content, has a prospect application in the processing of traditional Chinese medicine pharmacy, especially for concentrated pill.


Assuntos
Medicamentos de Ervas Chinesas/química , Água/química , Química Farmacêutica , Medicina Tradicional Chinesa
18.
Ying Yong Sheng Tai Xue Bao ; 27(12): 3759-3768, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-29704332

RESUMO

Based on 7-, 17-, 30-, and 40-year-old Larix kaempferi plantations, this paper studied the influence of tree age on biomass, carbon storage and nutrient characteristics. There was significant difference in biomass and nutrient concentration of stem, bark, needle, branch and root at different development stages at individual tree level. The biomass of each organ showed a trend of increase with the increasing age. The concentrations of N, P, K decreased, Mg concentration increased at first and then decreased, and Ca concentration continued to rise with the increasing age. There was significant difference in biomass of each organ for dominant, intermediate and suppressed trees, but no significant difference in nutrient concentration. It indicated that nutrient concentration of each organ was not affected by competition. At stand level, the total biomass, carbon storage and nutrient accumulation increased with the increasing age. Compared with young stand, the growth rate of biomass, carbon storage and nutrient accumulation were increased by 217%, 218% and 56% in mature stand, respectively. It indicated L. kaempferi had a high nutrient use efficiency, and could utilize less nutrient to produce more dry matter. Except that the accumulation of P and K had a slight decrease in pre-mature stand (30 years old), other elements increased with the increasing age. N mainly concentrated in needle, Ca concentrated in stem, K and Mg concentrated in root and P was distributed evenly in different organs. The annual accumulation rates of biomass, carbon and nutrient of L. kaempferi stands decreased with the increasing age, from 7.16 t·hm-2, 3.40 t·hm-2 and 104.64 kg·hm-2 for young stand to 3.99 t·hm-2, 1.89 t·hm-2and 28.64 kg·hm-2 for mature stand, respectively. It indicated that L. kaempferi plantations had great carbon sequestration potential and high nutrient consumption during young and middle ages.


Assuntos
Sequestro de Carbono , Florestas , Larix , Biomassa , Carbono , China , Raízes de Plantas , Caules de Planta , Solo , Árvores
19.
Appl Biochem Biotechnol ; 177(4): 957-66, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26265394

RESUMO

The synaptic protein alpha-synuclein (α-syn) is associated with a number of neurodegenerative diseases, and homology analyses among many species have been reported. Nevertheless, little is known about the cDNA sequence and protein structure of α-syn in tree shrews, and this information might contribute to our understanding of its role in both health and disease. We designed primers to the human α-syn cDNA sequence; then, tree shrew α-syn cDNA was obtained by RT-PCR and sequenced. Based on the acquired tree shrew α-syn cDNA sequence, both the amino acid sequence and the spatial structure of α-syn were predicted and analyzed. The homology analysis results showed that the tree shrew cDNA sequence matches the human cDNA sequence exactly except at nucleotide positions 45, 60, 65, 69, 93, 114, 147, 150, 157, 204, 252, 270, 284, 298, 308, and 324. Further protein sequence analysis revealed that the tree shrew α-syn protein sequence is 97.1 % identical to that of human α-syn. The secondary protein structure of tree shrew α-syn based on random coils and α-helices is the same as that of the human structure. The phosphorylation sites are highly conserved, except the site at position 103 of tree shrew α-syn. The predicted spatial structure of tree shrew α-syn is identical to that of human α-syn. Thus, α-syn might have a similar function in tree shrew and in human, and tree shrew might be a potential animal model for studying the pathogenesis of α-synucleinopathies.


Assuntos
DNA Complementar/genética , Tupaiidae/genética , alfa-Sinucleína/química , alfa-Sinucleína/genética , Sequência de Aminoácidos , Animais , Clonagem Molecular , Humanos , Dados de Sequência Molecular , Estrutura Secundária de Proteína , Homologia de Sequência do Ácido Nucleico
20.
Molecules ; 20(4): 6060-7, 2015 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-25856058

RESUMO

New simple sequence repeat (SSR) markers were developed in the Japanese larch (Larix kaempferi) using unigene sequences for further genetic diversity studies and the genetic improvement of breeding programs. One thousand two handred and thirty five (1235) primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the amplified candidate markers, 145 (90.6%) exhibited polymorphism among 24 individuals of L. kaempferi, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) averaging at 4.510, 0.487, 0.518 and 0.459, respectively. Cross-species amplification of randomly selection of 30 genic-SSRs among the 145 polymorphic ones showed that 80.0% of the SSRs could be amplified in Larix olgensis, 86.7% could be amplified in Larix principi-rupprechtii and 83.0% could be amplified in Larix gmelinii. High rates of cross-species amplification were observed. The genic-SSRs developed herein would be a valuable resource for genetic analysis of Larix kaempferi and related species, and also have the potential to facilitate the genetic improvement and breeding of larch.


Assuntos
DNA de Plantas , Marcadores Genéticos , Larix/genética , Repetições de Microssatélites , Polimorfismo Genético
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