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1.
Anim Biotechnol ; : 1-11, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32213006

RESUMO

Cattle fatty liver has caused mass damage in milk production during the past few years. In our study, to identify different miRNAs involved in cell physiological regulation in fatty liver, we performed miRNA deep sequencing on a normal liver cell (S01), fatty liver cell (S02) and processed cell by monoammonium glycyrrhizinate (S03). As a result, a total of 15,277,462, 14,190,360 and 13,771,060 raw reads representing 13,904,074, 12,784,128 and 11,017,604 clean reads per library were obtained separately. Through bioinformatics analysis, a total of 511 known miRNAs were identified when they were aligned with the known animal miRNAs, and 197 novel miRNAs were predicted using mirDeep2 software. A total of 511 miRNAs including 101 known and 51 novel miRNAs were expressed significantly different. Additionally, expression levels of eight randomly selected miRNAs were confirmed using the stem-loop qPCR, and their expression profiles were consistent with the deep sequencing results. For better understanding the functions of miRNAs, a total of 14,231 targets were predicted. These predicted target genes were further analyzed by function annotation and enrichment pathways, the results showed that these targets of the identified miRNAs are involved in a broad range of physiological functions.

2.
Genomics ; 111(6): 1315-1324, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-30196078

RESUMO

MicroRNAs (miRNAs) play important regulatory roles in numerous biological processes, but there is no report on miRNAs of hybrid snakehead. In this study, four independent small RNA libraries were constructed from the spleen, liver kidney and muscle of hybrid snakehead. These libraries were sequenced using deep sequencing technology, as result, a total of 1,067,172, 1,152,002, 1,653,941 and 970,866 clean reads from these four libraries were obtained. 252 known miRNAs and 63 putative novel miRNAs in these small RNA dataset were identified. The stem-loop RT-qPCR analysis indicated that eight known miRNAs and two novel miRNAs show different expression in eight different kinds of tissues. For better understanding the functions of miRNAs, 95,947 predicated target genes were analyzed by GO and their pathways, the results indicated that these targets of the identified miRNAs are involved in a broad range of physiological functions.

3.
PLoS One ; 13(5): e0196668, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29723230

RESUMO

BACKGROUND: China has a high rate of antibiotic use. The Chinese Ministry of Health (MOH) established the Center for Antibacterial Surveillance (CAS) to monitor the use of antibacterial agents in hospitals in 2005. The purpose of this study was to identify trends, pattern changes and regional differences in antibiotic consumption in 151 public general tertiary hospitals across China from 2011-2014. MATERIALS AND METHODS: Valid data for antibiotic use were collected quarterly, and the antibiotic consumption data were expressed as the defined daily dose (DDD) per 100 inpatient days (ID). We compared the patterns of antibiotic use in different classes and geographical clusters. RESULTS: Total antibiotic use significantly decreased (P = 0.018) from 75.86 DDD/100 ID in 2011 to 47.03 DDD/100 ID in 2014. The total consumption of flomoxef sodium and cefminox increased from 1.31 DDD/100 BD in 2011 to 8.6 DDD/100 BD in 2014. Cephalosporins were the most frequently used antibiotics in all regions. Third-generation cephalosporins accounted for more than 45% of the cephalosporins used. Carbapenem use substantially increased (P = 0.043). Penicillin combinations with inhibitors accounted for 50% of the penicillin used, and prescribed meropenem accounted for most of the carbapenems used in all regions in 2014. The subclasses in each antibiotic group were used differently between the seven regions, and the total hospital antibiotic use in 2014 differed significantly by region (P = 0.014). CONCLUSION: Although the volume and intensity of total antibiotic use decreased, the antibiotic use patterns were not optimal, and broad-spectrum antibiotics were still the main classes. The aggregate data obtained during the study period reveal similar antibiotic consumption patterns in different regions. These findings provide useful information for improving the rational use of antibiotics. More detailed data on antibiotics linked to inpatient diseases need to be collected in future studies.


Assuntos
Antibacterianos/uso terapêutico , Hospitais Gerais/estatística & dados numéricos , Hospitais Públicos/estatística & dados numéricos , Padrões de Prática Médica/estatística & dados numéricos , Centros de Atenção Terciária/estatística & dados numéricos , Antibacterianos/economia , Gestão de Antimicrobianos , China , Uso de Medicamentos/tendências , Humanos , Motivação , Padrões de Prática Médica/economia
4.
Gene ; 626: 298-304, 2017 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-28552712

RESUMO

MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play important roles in regulation of almost all biological processes in organisms. Topmouth culter is an economically important fish species in the Chinese freshwater polyculture system for its delicacy and high economic value. However, to date, no miRNAs have been reported in Topmouth culter species. In this study, the liver, muscle, spleen and kidney of Topmouth culter are sampled and used for construction of a small RNA library for deep sequencing. A total of 273 conserved miRNAs/miRNAs-star and 8 novel miRNAs/miRNAs-star were discovered, these miRNAs belong to 86 different miRNA families. There is a strong base bias on miRNA nucleotide compositions at certain positions. U is the dominant nucleotide, particularly at the beginning, middle, and end of conserved miRNAs. The expression of all 8 novel identified miRNAs in Topmouth culter was validated by using stem-loop qRT-PCR. Furthermore, the potential targets were predicted for these miRNAs, which were found to be involved in diverse biological processes. Our study is the first significantly enriches the Topmouth culter miRNA repertoire and provides a reference for further elucidation of complex miRNA-mediated regulatory networks for gene expression in the growth and developmental progression of Topmouth culter.


Assuntos
Cyprinidae/genética , MicroRNAs/genética , Animais , Sequência Conservada , Cyprinidae/crescimento & desenvolvimento , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Redes Reguladoras de Genes , MicroRNAs/química
5.
Comput Biol Chem ; 68: 92-100, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28282565

RESUMO

MicroRNAs (miRNAs) are post-transcriptional regulators which bind to target to regulate protein expression by repressing translation or promoting degradation of the target mRNA. Studies have shown that deep sequencing is a powerful tool for the identification of miRNAs, and it is believed that may more miRNAs remain to be discovered in grass carp. In the present study, a pool of equal amounts of RNA obtained from 8 different adult grass carp tissues (spleen, liver, muscle, kidney, skin, testis, gut and heart) was sequenced using deep sequencing technology. A total of 16.579.334 raw reads were yielded from the pooled small RNA library. Using bioinformatics analysis, we identified 160 conserved miRNAs and 18 novel miRNAs in grass carp. Randomly selected 6 conserved and 2 novel miRNAs were confirmed their expression by stem-loop qRT-PCR assay. Furthermore, the 1212 potential targets of these miRNAs were predicted using miRNA target prediction tool. GO and KEGG pathway enrichment analyses indicated relevant biological processes. Our study provides the first genome-wide investigation of miRNAs from 8 mixed tissues of grass carp, and the data obtained expand the known grass carp miRNA inventory and provide a basis for further understanding functions of grass carp miRNAs.


Assuntos
Carpas/genética , Sequência Conservada/genética , Sequenciamento de Nucleotídeos em Larga Escala , MicroRNAs/genética , Animais
7.
Sci Rep ; 5: 7674, 2015 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-25567703

RESUMO

There has been considerable professional debate on the association between nausea and vomiting in early pregnancy (NVP) and neural tube defects (NTDs) risk. This study explored the association between NVP and NTDs risk, and the effect of folic acid supplements on the association. A 1:1 matched case-control study was conducted and conditional logistic regression model was used to analyze the associations. The result showed the odds ratio (OR) of severe NVP for NTDs was 2.403 (95%CI 1.437,4.017; P<0.001) and that of moderate NVP was 1.469 (95%CI 1.063,2.031; P = 0.020) compared with light NVP when adjusted by the potential confounders. Stratified by intake of folic acid supplements, the ORs for severe and moderate NVP turned to 2.147 (95%CI 1.140, 4.043; P = 0.018) and 2.055 (95%CI 1.320, 3.199; P = 0.001) in the stratum of non-intake of folic acid supplements while ORs reduced to 1.851 (95%CI 0.729, 4.699; P = 0.195) and 1.003 (95%CI 0.594, 1.694; P = 0.992) in the stratum of intake of folic acid supplements, respectively. We conclude that severe/moderate NVP has an association with the risk of NTDs, which was not found in the group with intake of folic acid supplements. Folic acid supplements should be recommended to use for the prevention of NTDs.


Assuntos
Ácido Fólico/uso terapêutico , Náusea/etiologia , Defeitos do Tubo Neural/prevenção & controle , Vômito/etiologia , Adulto , Estudos de Casos e Controles , Suplementos Nutricionais , Feminino , Humanos , Modelos Logísticos , Defeitos do Tubo Neural/patologia , Razão de Chances , Gravidez , Fatores de Risco , Índice de Gravidade de Doença
8.
Acta Paediatr ; 102(9): 908-13, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23750819

RESUMO

AIM: To study the influence of maternal body mass index (BMI) on the association between folic acid supplementation and neural tube defects (NTDs) risk in offspring. METHODS: A hospital-based, case-control study was conducted between 2006 and 2008 on 459 mothers with NTDs-affected births and 459 mothers without NTDs-affected births. Logistic regression models examined the associations between folic acid supplementation and the NTDs risk in offspring for all mothers, underweight/normal weight mothers (BMI<24.0) and overweight/obese mothers (BMI ≥24.0). The effects were evaluated by adjusted odds ratio (AOR) and 95% confidence intervals (CIs) with SAS 9.1.3 software. RESULTS: The overall AOR for periconceptional folic acid supplementation was 0.315 (95% CI = 0.172-0.577) when compared with no supplements. Stratified by maternal BMI, the AOR for periconceptional folic acid supplementation in overweight/obese mothers was greater than in underweight/normal weight mothers (0.646 vs. 0.208). The AOR for folic acid supplementation within 3 months before conception was 0.711 (95% CI = 0.323-1.563) in all mothers. Stratified by maternal BMI, the AOR for folic acid supplementation within 3 months before conception in overweight/obese mothers was greater than in underweight/normal weight mothers (0.658 vs. 0.527). CONCLUSION: The association between folic acid supplementation and the reduced NTDs risk was weaker in overweight/obese mothers (BMI ≥24.0) than in underweight/normal weight mothers (BMI <24.0).


Assuntos
Índice de Massa Corporal , Suplementos Nutricionais , Ácido Fólico/administração & dosagem , Defeitos do Tubo Neural/prevenção & controle , Obesidade/epidemiologia , Adulto , Análise de Variância , Intervalos de Confiança , Estudos Transversais , Feminino , Humanos , Incidência , Recém-Nascido , Modelos Logísticos , Masculino , Bem-Estar Materno , Defeitos do Tubo Neural/epidemiologia , Razão de Chances , Gravidez , Cuidado Pré-Natal/métodos , Prognóstico , Valores de Referência , Medição de Risco , Adulto Jovem
9.
Birth Defects Res A Clin Mol Teratol ; 97(3): 161-5, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23483719

RESUMO

BACKGROUND: Studies have suggested that being obese before pregnancy is a risk factor for neural tube defects (NTDs), while results of many studies are controversial. This study aims to explore the association between maternal overweight and obesity before pregnancy and risk of NTDs. METHODS: A 1:1 matched case-control study was conducted. Cases were 459 women who delivered infants or conceived fetuses with NTDs in two provinces of China and controls were 459 women with live-born infants, without an apparent congenital malformation, matched with cases by region, delivery hospital, and year of childbirth. RESULTS: Compared with normal body weight, maternal obesity (body mass index, BMI ≥ 28 kg/m(2)) before pregnancy had a significant increased risk for NTDs as a group after adjusting for age, occupation, educational level, family income, parity, and use of folic acid (odds ratio, OR = 2.45; p < 0.05). No significant increase in NTD risk was found for maternal underweight (BMI < 18.5 kg/m(2)) and overweight (BMI from 24 to 28 kg/m(2)). For three subtypes of NTDs, anencephaly, spina bifida, and encephalocele, no significant increase in risk was found with maternal underweight, overweight, or obesity (all, p > 0.05). CONCLUSION: Maternal obesity before pregnancy is associated with risk of NTDs and should be considered in maternal reproductive health care.


Assuntos
Anencefalia/epidemiologia , Encefalocele/epidemiologia , Obesidade/epidemiologia , Sobrepeso/epidemiologia , Disrafismo Espinal/epidemiologia , Adulto , Índice de Massa Corporal , Estudos de Casos e Controles , China/epidemiologia , Feminino , Humanos , Mães , Gravidez , Risco , Fatores de Risco
10.
J Reprod Med ; 56(9-10): 431-6, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22010529

RESUMO

OBJECTIVE: To further explore the association between abortion history and neural tube defect (NTD) risk and the association of different interpregnancy intervals from prior abortion to current conception on NTD risk. STUDY DESIGN: A matched case-control study was conducted. Cases were 459 women who delivered and gestated babies/fetuses with NTDs in two provinces of China and 459 women with live-born infants, without an apparent congenital malformation, matched with cases by the same region, the same hospital, and childbirth in the same year as controls. All of the subjects were investigated by trained interviewers with the same criteria. Conditional logistic regression models were used to calculate odds ratios (ORs) and their 95% CI of abortion history and interpregnancy intervals on NTD risk. RESULTS: Women with abortion history did not have an increased risk for spina bifida and encephalocele. The interpregnancy interval of < 6 months from prior abortion to current conception was significantly associated with anencephaly risk. CONCLUSION: Abortion with an interpregnancy interval of < 6 months from prior abortion to current conception may have an increased risk for anencephaly and should be considered in maternal reproductive health care.


Assuntos
Aborto Induzido , Aborto Espontâneo/epidemiologia , Defeitos do Tubo Neural/epidemiologia , História Reprodutiva , Adulto , Estudos de Casos e Controles , China , Estudos de Coortes , Feminino , Humanos , Fatores de Risco , Fatores Socioeconômicos , Fatores de Tempo , Adulto Jovem
11.
Birth Defects Res A Clin Mol Teratol ; 88(9): 737-42, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20589881

RESUMO

BACKGROUND: Measures for prevention of neural tube defects (NTDs) have been recommended for many years in China, but the compliance with these measures is unsatisfactory. This study aims to compare the effect differences between planned pregnancy and unplanned pregnancy in the compliance with these measures and analyze the interactions between pregnancy planning and these measures for NTD prevention. METHODS: A 1:1 matched case-control study was conducted. We randomly selected 349 women who delivered or gestated babies/fetuses with NTDs in the last two years in two provinces and matched them with 349 women who delivered babies without obvious birth defects as controls. RESULTS: In the case group, 99 women reported that they had planned their pregnancies, accounting for 28.4%, and the proportion who received preconception examinations and took folic acid prior to conception was 13.8 and 8.6%, respectively. According to the multivariate analysis, health education (odds ratio [OR], 0.350), preconception examinations (OR, 0.497) and folic acid consumption prior to conception (OR, 0.257) all had preventative effects on NTDs (for all, p < 0.05). In both groups, the proportions of women who received preconception examinations and reported folic acid intake were much higher for those who reported planning their pregnancies compared to women with an unplanned pregnancy (for all, p < 0.01); and for NTD prevention, synergistic interactions existed between pregnancy planning and the other preventive measures. CONCLUSION: Folic acid consumption prior to conception, preconception examinations, and health education have preventive effects on NTDs. Pregnancy planning can significantly promote compliance with these preventive behaviors. In addition, there are synergistic interactions between pregnancy planning and these measures.


Assuntos
Serviços de Planejamento Familiar , Ácido Fólico/uso terapêutico , Defeitos do Tubo Neural/prevenção & controle , Cuidado Pré-Concepcional , Gravidez não Planejada , Cuidado Pré-Natal , Estudos de Casos e Controles , China , Anticoncepção/efeitos adversos , Suplementos Nutricionais , Feminino , Comportamentos Relacionados com a Saúde , Humanos , Gravidez , Gravidez não Desejada , Vitaminas/uso terapêutico
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