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Zhongguo Zhong Yao Za Zhi ; 44(1): 141-149, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30868825


This study aims to observe the intervention effects of Chinese herbal medicine of supplementing Qi and activating blood circulation on chronic intermittent hypoxia(CIH) composite insulin resistance(IR) mediated atherosclerosis(AS) mice model,and to observe the mechanism of SREBP-1 c signaling molecule.IR Apo E-/-mice model was induced by high-fat diet combined with STZ injection.Then the mice were treated with hypoxic animal incubator for 8 h per day and 8 weeks to establish a CIH+IR-ApoE-/-mouse model.Model mice were randomly and averagely divided into normoxic control group(NC),model group(CIH) and SREBPs inhibitor group(betulin),atorvastatin group(WM),TCM low-dose group(TCM-L),TCM middle-dose group(TCM-M) and TCM high-dose group(TCM-H) group.Chinese herbal medicine of supplementing Qi and activating blood circulation including ginsenosides combined with ligustrazine(TMP) were used as intervention drugs.The study observed the effect of drugs on IR,serum lipid,inflammation,stress,AS and SREBP-1 c related molecules.The results showed that fasting blood glucose in TCM-H group decreased compared with other experimental groups(P<0.05).HDL-C level in betulin group,WM group,TCM-H group was higher than that in CIH group(P<0.05).LDL-C level in TCM-M group,TCM-H group is lower than that in CIH group(P<0.05).The level of CRP in CIH group was higher than that in other groups(P<0.05).The level of SOD in TCM-H group was higher than that in CIH group(P<0.05).NC group and CIH group showed obvious AS aortic plaque,while betulin group,WM group,TCM-H group showed reduction in AS plaque(P<0.05).For descending aorta,AS plaque in CIH group was multiple and large,while less and smaller in WM group and TCM-H(P<0.05).The expression of SREBP-1 c and FAS in aorta and skeletal muscle in TCM-H group was lower than that in CIH group(P<0.05).In aorta,the expression of TNF-α and CD106(VCAM-1) was lower in TCM-H group than that in CIH group(P<0.05).In aorta,skeletal muscle and liver,the level of p-IRS-1 in TCM-H group was significantly higher than that in CIH group(P<0.05).In aorta and liver,the expression of HIF-1α in TCM-H group was lower than that in CIH group(P<0.05).The study demonstrated that combination ginsenosides with TMP could improve IR and serum lipid level and inhibit inflammation and oxidative stress as well as ultimately alleviate AS to some extent.And the mechanism of its interventional effects might be related to the inhibition of CIH-induced upregulation of SREBP-1 c related molecules.

Aterosclerose/tratamento farmacológico , Circulação Sanguínea/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Resistência à Insulina , Qi , Animais , Ginsenosídeos/farmacologia , Hipóxia/patologia , Camundongos , Camundongos Knockout para ApoE , Pirazinas/farmacologia , Distribuição Aleatória
J Proteomics ; 177: 31-39, 2018 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-29425737


Bacillus nematocida B16 (B16) is a pathogenic bacterium that is nematotoxic to plant-parasitic nematodes. In this study, we performed a quantitative lysine acetylome analysis on B16 to understand the potential roles of protein lysine acetylation on this host-pathogen interaction. Altogether, we identified 529 acetylation sites in 349 proteins, quantified 411 sites in 288 proteins, determined that the acetylation levels of 18 sites were up-regulated and those of 19 sites were down-regulated during pathogenesis. The acetylated proteins mainly participated in metabolic processes, protein synthesis, and cell wall/membrane biogenesis. Moreover, these proteins are involved in more than twenty KEGG pathways. Eight peptide motifs of acetylated proteins were identified, five of which have been thus far found only in the B16 acetylome. Twenty-two acetylated proteins were found to be involved in the synthesis of nematode attractants, and two were found to be involved in the secretion of virulence factors. In addition, the acetylation levels of ten lysine sites were regulated significantly differently in the presence of nematodes. Our results reveal that lysine acetylation may play roles in regulating B16-nematode interaction. SIGNIFICANCE: B. nematocida B16 is a bio-control bacterium against nematodes. It lures nematodes to their death by a Trojan horse mechanism. But there is little understanding about the regulation of this "Trojan horse" like pathogenesis. Lysine acetylation was reported to regulate diverse cellular processes. Our results revealed that lysine acetylation played indeed roles in regulating the B16-nematodes interaction. Our data laid a foundation for studying the molecule mechanism of lysine acetylation in regulating this host-pathogen interaction.

Bacillus/patogenicidade , Interações Hospedeiro-Patógeno , Lisina/metabolismo , Nematoides/microbiologia , Acetilação , Animais , Proteínas de Bactérias/metabolismo , Regulação Bacteriana da Expressão Gênica
Artigo em Inglês | MEDLINE | ID: mdl-12168047


A new initiator system for polyacrylamide gel polymerization at low pH is developed. The system consists of ascorbic acid, ferrous sulfate and ammonium persulfate (AP). It is effective under acid conditions even if the concentration of the initiators is very low. To get high incorporation efficiency of acrylamide, the ratio of the AP, ascorbic acid and ferrous sulfate must be in a suitable range. The conversion efficiency has been assessed as a function of the pH of the gelling solution, in pH2.0--6.58 range. New system is able to guarantee>98% conversion in the pH 2.0--5.0 range. In contrast to the hydrogen peroxide-ascorbic acid-ferrous sulfate system widely used under acid conditions, new system gives a much higher incorporation efficiency of acrylamide, lower gel pore size at the same concentration and cross linkage and better gel mechanical property that made the gel easy handle for general purpose. The recommended concentrations of the initiators are: AP, 2.2-3.3 mmol/L, ascorbic acid, 0.91-1.82mmol/L, FeSO(4), 0.046-0.092 mmol/L.