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1.
Food Chem ; 366: 130572, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34284190

RESUMO

In this study, a fluorine-fluorine interaction approach through fluoridating boron nitride nanosheets (BNNs) for sensing perfluoroalkyl acids (PFAAs) in multiple food matrices was developed. Through a facile hydrothermal fluorination modification, the BNNs were transferred into homogeneous fluorinated boron nitride nanoparticles (F-BNNs) with robust networks and specific surface area. After morphological modification, the particles displayed strong adsorption and sensing capabilities on PFAAs in both solid and liquid food matrix. Under the evaluation of mass spectrometry, F-BNNs based microextraction approach exhibited low method detection limits (MDLs) in the ranges of 0.9-3.9 pg mL-1 and 3.6-15.8 pg g-1 for milk and meat matrices, respectively, with satisfactory repeatability (RSD% <13.5%) and recoveries (77.7-110.5%). This work not only depicted a facile approach for preparing F-BNNs based SPME fiber, but also provided a routine analysis protocol for monitoring PFAAs in food systems.


Assuntos
Flúor , Fluorcarbonetos , Cromatografia Líquida , Microextração em Fase Sólida , Espectrometria de Massas em Tandem
2.
Biosens Bioelectron ; 195: 113682, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34624800

RESUMO

Accurate, sensitive, and rapid detection of Salmonella and determination of whether it carries drug resistance genes plays an important role in guiding the clinical medication of salmonellosis and laying a foundation for studying the mechanism of drug resistance transmission of Salmonella. Here, a novel nontransferable, ultrasensitive dual detection platform (Cas12a-Ddp) was developed. The round cap allowed for temporary storage of more Cas12a detection solution than flat cap, enabling one-pot assays and reducing aerosol contamination. The results were read out in dual mode by the microplate reader and UV visualization to achieve sensitive dual-target detection of the virulence genes and drug resistance genes of Salmonella simultaneously, with the possibility of onsite detection. Cas12a-Ddp was combined with multiple polymerase chain reactions and recombinase polymerase amplifications successively. An ultrasensitive dual detection limit of 1 CFU/mL was obtained without any cross-reaction within 40 min. This was an improvement of 1-2 orders of magnitude over the existing methods. Cas12a-Ddp overcame the influence of proteins and fat in liquid matrix foods. It was used for the detection of drug-resistant Salmonella in milk and skim milk powder, also with the dual detection limit of 1 CFU/mL and spiked recovery of 68.58%-158.49%. It was also used for the analysis of Salmonella resistance rate analysis. The Cas12a-Ddp provided a reliable, fast, sensitive, and practical multi-CRISPR detection platform.

3.
Sci Total Environ ; 807(Pt 1): 150840, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34627904

RESUMO

The toxicity evaluation suffers from the absence of suitable models capable of replicating in the co-cultured cell microenvironment and the function of specific tissues in vitro. Motivated by this urgent need, this study aimed to describe a novel three-dimensional (3D) liver spheroid model. The model consisted of a triple co-culture of HepG2, EA.hy 926, and LX-2. Subsequently, it was used for the toxicity evaluation of aflatoxin B1 (AFB1), and its advantages over the two-dimensional (2D) model and the mono-type cell spheroid model were assessed. This study examined the effects of AFB1 on cell viability, proliferation, mitochondria, oxidative stress, and cell membranes. The results revealed that AFB1 greatly affected 2D cell membranes and oxidative stress levels (0.01 µg/mL; 24 h), and could also significantly affect 2D cell viability, proliferation, and mitochondria levels (1 µg/mL; 24 h). On the contrary, 3D cells were less susceptible to AFB1. Combined with the analysis of gene expression, both metabolic activation (cytochrome P450; CYP450) and detoxification efficiency (drug-metabolizing enzymes) were found to be higher in 3D cells than in 2D cells. Moreover, 3D cells in triple co-culture outperformed mono-type cell spheroids. Therefore, the advanced 3D co-cultured spheroid model constructed in this study allowed us to more realistically simulate the microenvironment in vitro, and was a valuable and precise model to study mycotoxins.

4.
J Cell Mol Med ; 25(20): 9753-9766, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34514714

RESUMO

Oridonin, a natural diterpenoid compound extracted from a Chinese herb, has been proved to exert anti-oxidative stress effects in various disease models. The aim of the present study was to investigate the protective effects of oridonin on oxidative stress-induced endothelial injury in ischaemic stroke. We found oridonin repaired blood-brain barrier (BBB) integrity presented with upregulation of tight junction proteins (TJ proteins) expression, inhibited the infiltration of periphery inflammatory cells and neuroinflammation and thereby reduced infarct volume in ischaemic stroke mice. Furthermore, our results showed that oridonin could protect against oxidative stress-induced endothelial injury via promoting nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf-2). The specific mechanism could be the activation of AKT(Ser473)/GSK3ß(Ser9)/Fyn signalling pathway. Our findings revealed the therapeutic effect and mechanism of oridonin in ischaemic stroke, which provided fundamental evidence for developing the extracted compound of Chinese herbal medicine into an innovative drug for ischaemic stroke treatment.

5.
Food Chem ; 370: 131027, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34537432

RESUMO

The pollution caused by estrogens in the environment and food has received increasing attention. It is still challenging for on-site immunochromatographic assay (ICA) detection of estrogens. The performance of the prepared probes plays a decisive role in the sensitivity and stability of the ICA system. The published probes usually directly couple the detection antibody to the label, ignoring the influence of the label on the activity of the antibody. In this study, 17ß-estradiol (E2) was used as a model analyte for the ICA system. Two universal probes were constructed based on quantum dot nanobeads (QBs), recombinant protein A (SPA, from Staphylococcus aureus), and rabbit anti-mouse immunoglobulin G antibody (anti-IgG). The probes were prepared by coupling QBs with SPA, releasing anti-E2 monoclonal antibody (mAb), and maintaining its activity. The prepared universal probes can orient recognize the Fc region of mAb and fully expose its Fab region, improving the detection sensitivity of the ICA system. The free anti-E2 mAb and the universal probe (QBs@SPA or QBs@SPA@anti-IgG) were used as the detection antibodies and signal donors, respectively. The results show that the proposed ICA based on QBs@SPA and QBs@SPA@anti-IgG probes could detect E2 with IC50 of 8.83 and 0.93 ng/mL, respectively, within 15 min under optimal conditions. The recovery results of ICA based on QBs@SPA and QBs@SPA@anti-IgG probes showed good agreement with the findings of the high-performance liquid chromatography (HPLC) analysis for spiked samples. The developed ICA system based on universal probes was superior in terms of sensitivity, rapidity, and applicability, and held great promise for its implementation in detecting environmental and food small-molecule pollutants.

6.
Talanta ; 234: 122671, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34364472

RESUMO

Traditional cell biology researches on cell populations by their origin, tissue, morphology, and secretions. Because of the heterogeneity of cells, research at the single-cell level can obtain more accurate and comprehensive information that reflects the physiological state and process of the cell, increasing the significance of single-cell analysis. The application of single-cell analysis is faced with the problem of contaminated or damaged cells caused by cell sample transportation. Reversible encapsulation of a single cell can protect cells from the external environment and open the encapsulation shell to release cells, thus preserving cell integrity and improving extraction efficiency of analytes. Meanwhile, microfluidic single cell analysis (MSCA) exhibits integration, miniaturization, and high throughput, which can considerably improve the efficiency of single-cell analysis. The researches on single-cell reversible encapsulation materials, single-cell analysis methods, and the MSCA integration platform are analyzed and summarized in this review. The problems of single-cell viability, network of single-cell signal, and simultaneous detection of multiple biotoxins in food based on single-cell are proposed for future research.


Assuntos
Técnicas Analíticas Microfluídicas , Microfluídica , Humanos , Análise de Célula Única
7.
Anal Bioanal Chem ; 413(26): 6489-6502, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34430984

RESUMO

Deoxynivalenol (DON) and zearalenone (ZEN) are mycotoxins that contaminate a wide range of grains and crops. In this study, a one-step time-resolved single-channel immunochromatographic test strip based on europium ion polystyrene fluorescence microspheres was first developed for sensitive and quantitative detection of DON and ZEN. The concentration of the artificial antigen and the mass ratio of the monoclonal antibody to fluorescent microspheres for conjugation were optimized to simplify the sample addition process during immunochromatographic assay and improve the on-site detection efficiency. The limits of detection (LOD) of the single-channel immunochromatographic test strip for DON and ZEN detection were 0.17 and 0.54 µg/L, respectively. Meanwhile, the dual-channel immunochromatographic test strip was designed to simultaneously detect DON and ZEN, with LODs of 0.24 and 0.69 µg/L achieved for DON and ZEN, respectively. The developed test strips also yielded recovery results consistent with that obtained by LC-MS/MS for DON and ZEN detection in real samples of wheat and corn flour, confirming the practicability and reliability of the test strip. The developed immunochromatographic test strips realize quick and sensitive detection of DON and ZEN, exhibiting potential for broad applications in the point-of-care testing platform of multiple mycotoxins in agricultural products. Graphic abstract.

8.
Food Chem ; 365: 130409, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34256225

RESUMO

A new, green, and cost-effective magnetic solid-phase extraction of aflatoxins and ochratoxins from edible vegetable oils samples was developed using polydopamine-coated magnetic multi-walled carbon nanotubes (PDA@Fe3O4-MWCNTs) as the absorbent. PDA@Fe3O4-MWCNTs nanomaterials were prepared by chemical co-precipitation and in situ oxidation and self-polymerization of dopamine and was characterized. Factors affecting MSPE and the adsorption behavior of the adsorbent to mycotoxins were studied, and the optimal extraction conditions of MSPE and the complexity of the adsorption process were determined. Based on this, the magnetic solid-phase extraction-high-performance liquid chromatography-fluorescence detection method (MSPE-HPLC-FLD) was established for determining six mycotoxins [aflatoxin B1 (AFB1), AFB2, AFG1, and AFG2, and ochratoxin A (OTA) and OTB)] in vegetable oils. The recovery was 70.15%~89.25%, and RSD was ≤6.4%. PDA@Fe3O4-MWCNTs showed a high affinity toward aflatoxins and ochratoxins, allowing selective extraction and quantification of aflatoxins and ochratoxins from complex sample matrices.


Assuntos
Aflatoxinas , Nanotubos de Carbono , Ocratoxinas , Adsorção , Aflatoxinas/análise , Cromatografia Líquida de Alta Pressão , Dopamina , Contaminação de Alimentos/análise , Fenômenos Magnéticos , Ocratoxinas/análise , Óleos Vegetais , Verduras
9.
Addict Biol ; 26(6): e13062, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34114299

RESUMO

Methamphetamine (METH) abuse has become a global public health problem. However, the potential mechanisms involving METH-induced metabolic disorders have thus far remained poorly understood. Metabolomics can provide a clue for the cause of apparent changes and consequently be used to investigate the METH-induced dysregulation of metabolite expression and the mechanism of metabolic disorder mechanism. This laboratory investigation included 80 METH abusers and 80 healthy people. The serum metabolites were detected and analysed by gas chromatography/time-of-flight mass spectrometry. Raw data were processed with the software MS DIAL, which includes deconvolution, peak alignment and compound identification. The data matrix was processed by univariate and multivariate analyses for significant metabolite screening with the criteria of variable importance in projection values > 1, fold change > 1.5 and the t test (p value < 0.05). Significant differences in 16 metabolites (deoxycholic acid, cholic acid, hydroxylamine, etc.) in serum were found between the METH abuse group and the control group. Energy metabolic pathways and several amino acid metabolic pathways (alanine, aspartic acid and glutamate metabolism and tryptophan metabolism) were primarily involved. Further analysis indicated that the area under the receiver operating characteristic curve (AUC) was 0.998 for these 16 metabolites. Among the metabolites, three carbohydrates (d-ribose, cellobiose and maltotriose) had an AUC of 0.975, which were determined as potential markers of abuse. We observed metabolic disturbances in METH abusers, particularly perturbation in energy metabolism and amino acid metabolism, which can provide new insights into the search for biomarkers and the mechanisms underlying the adverse effects of METH on human health.

10.
Food Res Int ; 144: 110359, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34053552

RESUMO

Antibiotic resistance in Salmonella enterica serovar Typhimurium (S. ser. Typhimurium) has become a critical safety hazard in food. Sublethal environmental stresses can influence resistance in Salmonella during food processing. This study simulated environmental stresses in food processing. The antibiotic resistance of three strains of S. ser. Typhimurium (the ATCC 14028 strain and two wild-type isolates from chicken and pork product processing) was evaluated under different pH levels (5.0, 5.5, 6.0, 8.0, and 9.0). Also, dynamic changes in resistance with treatment duration under cold (4 °C, -20 °C) and heat (55 °C) treatment were studied. The results showed that acid and alkaline stresses reduced the resistance of S. ser. Typhimurium to eight antibiotics; meanwhile, the resistance of meropenem (MERO) increased. The minimal inhibitory concentration (MIC) of MERO was increased 16- to 64-fold. With acid or alkaline stress, the extracellular ATP content increased, and the scanning electron microscopy (SEM) result clearly revealed the appearance of wrinkles and holes on the outer membrane of Salmonella. These observations imply changes in membrane permeability, which may decrease the antibiotic resistance of Salmonella. Cold or heat stress increased the resistance of S. ser. Typhimurium to tetracycline, cefotaxime, ceftazidime, nalidixic acid, azithromycin, and ampicillin; the MIC increased 2- to 4-fold. The antibiotic resistance only changed when cold and heat stresses occurred over a certain period of time and remained unchanged when the stress persisted. This study reports on the ability of S. ser. Typhimurium to develop antibiotic resistance after environmental stresses. It can provide valuable information for meat processing to improve interventions and risk management.


Assuntos
Farmacorresistência Bacteriana Múltipla , Salmonella typhimurium , Resposta ao Choque Térmico , Testes de Sensibilidade Microbiana , Sorogrupo
11.
FASEB J ; 35(5): e21581, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33871072

RESUMO

Synaptic plasticity damages play a crucial role in the onset and development of depression, especially in the hippocampus, which is more susceptible to stress and the most frequently studied brain region in depression. And, mitochondria have a major function in executing the complex processes of neurotransmission and plasticity. We have previously demonstrated that Iptakalim (Ipt), a new ATP-sensitive potassium (K-ATP) channel opener, could improve the depressive-like behavior in mice. But the underlying mechanisms are not well understood. The present study demonstrated that Ipt reversed depressive-like phenotype in vivo (chronic mild stress-induced mice model of depression) and in vitro (corticosterone-induced cellular model). Further study showed that Ipt could upregulate the synaptic-related proteins postsynaptic density 95 (PSD 95) and synaptophysin (SYN), and alleviated the synaptic structure damage. Moreover, Ipt could reverse the abnormal mitochondrial fission and fusion, as well as the reduced mitochondrial ATP production and collapse of mitochondrial membrane potential in depressive models. Knocking down the mitochondrial ATP-sensitive potassium (Mito-KATP) channel subunit MitoK partly blocked the above effects of Ipt. Therefore, our results reveal that Ipt can alleviate the abnormal mitochondrial dynamics and function depending on MitoK, contributing to improve synaptic plasticity and exert antidepressive effects. These findings provide a candidate compound and a novel target for antidepressive therapy.


Assuntos
Depressão/tratamento farmacológico , Canais KATP/antagonistas & inibidores , Mitocôndrias/efeitos dos fármacos , Propilaminas/farmacologia , Estresse Psicológico/complicações , Sinapses/efeitos dos fármacos , Animais , Depressão/etiologia , Depressão/patologia , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/metabolismo , Plasticidade Neuronal , Sinapses/metabolismo
12.
Crit Rev Food Sci Nutr ; : 1-21, 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33830835

RESUMO

With the gradually increasing prevalence of food allergy in recent years, food allergy has become a major public health problem worldwide. The clinical symptoms caused by food allergy seriously affect people's quality of life; there are unknown allergen components in novel food and hidden allergens caused by cross contamination in food processing, which pose a serious risk to allergy sufferers. Thus, rapid and multiplex detection methods are required to achieve on-site detection or examination of allergic components, so as to identify the risk of allergy in time. This paper reviews the progress of high-efficiency detection of food allergens, including enhanced traditional detection techniques and emerging detection techniques with the ability high-throughput detection or screening potential food allergen, such as xMAP, biosensors, biochips, etc. focusing on their sensitivity, applicability of each method in food, along with their pretreatment, advantages, limitation in the application of food analysis. This paper also introduces the challenges faced by these high-efficiency detection technologies, as well as the potential of customized allergen screening methods and rapid on-site detection technology as future research directions.

13.
Crit Rev Food Sci Nutr ; : 1-19, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33663294

RESUMO

Mycotoxins are metabolites produced by fungi growing in food or feed, which can produce toxic effects and seriously threaten the health of humans and animals. Mycotoxins are commonly found in food and feed, and are of significant concern due to their hepatotoxicity, nephrotoxicity, carcinogenicity, mutagenicity, and ability to damage the immune and reproductive systems. Traditional physical and chemical detoxification methods to treat mycotoxins in food and feed products have limitations, such as loss of nutrients, reagent residues, and secondary pollution to the environment. Thus, there is an urgent need for new detoxification methods to effectively control mycotoxins and treat mycotoxin pollution. In recent years, microbial detoxification technology has been widely used for the degradation of mycotoxins in food and feed because this approach offers the potential for treatment with high efficiency, low toxicity, and strong specificity, without damage to nutrients. This article reviews the application of microbial detoxification technology for removal of common mycotoxins such as Aflatoxin, Ochratoxin, Zearalenone, Deoxynivalenol, and Fumonisins, and discusses the development trend of this important technology.

14.
Bioelectrochemistry ; 139: 107743, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33524655

RESUMO

A "honeycomb" electrochemical biosensor based on 3D printing was developed to noninvasively monitor the viability of 3D cells and evaluate the individual or combined toxicity of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON). Carbon nanofiber (CN)/gelatin methacryloyl (GelMA) conductive composite hydrogel with strong processability was printed on 8-channel screen-printed carbon electrodes (SPCEs) to maintain cell viability and form tight cell-to-cell contacts. A "3D honeycomb" printing infill pattern was selected in the construction of the biosensors to improve conductivity. Based on 3D printing technology, the electrochemical biosensor can prevent manual error and provide for high-throughput detection. Electrochemical impedance spectroscopy (EIS) was used to evaluate mycotoxin toxicity. The EIS response decreased with the concentration of DON, 3-ADON and 15-ADON in the range of 0.1-10, 0.05-100, and 0.1-10 µg/mL, respectively, with a limit of detection of 0.07, 0.10 and 0.06 µg/mL, respectively. Mycotoxin interactions were analyzed using the isobologram-combination index (CI) method. The electrochemical cytotoxicity evaluation result was confirmed by biological assays. Therefore, a novel method for evaluating the combined toxicity of mycotoxins is proposed, which exhibits potential for application to food safety and evaluation.


Assuntos
Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Venenos/análise , Tricotecenos/análise , Células A549 , Carbono/química , Sobrevivência Celular , Eletrodos , Gelatina/química , Humanos , Hidrogéis/química , Nanofibras/química , Impressão Tridimensional , Espécies Reativas de Oxigênio/análise
15.
Sci Total Environ ; 771: 144823, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33545470

RESUMO

With increased interest in the toxic interactions of multiple toxins, biotoxicity models have to be urgently developed for joint toxicity evaluation. This study aimed to develop an optical biosensor based on living mammary cells for monitoring of cadmium (Cd)/deoxynivalenol (DON) in water and evaluating their combined toxicity. Our previous survey found that DON and Cd appeared simultaneously in various products, and RNA seq revealed that AP-1 participated in combined toxicity of DON+Cd in HT-29 cells. Thus AP-1 site-mCherry-based biosensors were constructed, optimized, and then tested for their applicability and stable fluorescence response activities. DON+Cd2+, DON, and Cd2+ induced dose-dependent fluorescence signal in the biosensors (at environmental exposure levels). The enhanced fluorescence signal suggested that the toxicity of DON+Cd2+ was enhanced compared with that of single toxin. The advantages of the biosensors include: I) The easy and visual screening of multiple toxins on the basis of environmental exposure levels; II) Potential as a broad-spectrum tool for joint toxicity evaluation of DON+Cd; III) Pollution-free and stable fluorescence response; IV) A slight effect on viability.


Assuntos
Técnicas Biossensoriais , Tricotecenos , Animais , Cádmio/toxicidade , Tricotecenos/toxicidade
16.
Analyst ; 146(4): 1444-1454, 2021 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-33410840

RESUMO

Single-cell electrochemical sensor is widely used in the local selective detection of single living cells because of its high spatial-temporal resolution and sensitivity, as well as its ability to obtain comprehensive cellular physiological states and processes with increased accuracy. Functionalized nanoprobes can detect the oxidative stress response of cells in single-cell electrochemical sensors. Moreover, the T-2 toxin is one of the most toxic mycotoxins and widely occurs in field crops. T-2 toxin can cause mitochondrial damage in cells and increase intracellular reactive oxygen species (ROS) in various cells. As the most representative free radical of intracellular ROS, H2O2 can effectively reflect the toxic effects of intracellular T-2 toxin. In this study, a functionalized gold nanoprobe was used to dynamically monitor the production of H2O2 in a single live human hepatoma cell HepG2 stimulated by mycotoxin T-2. The concentration of H2O2 produced by HepG2 cells stimulated by T-2 toxin at 1 ppb-1 ppm was linearly correlated, R2 = 0.99055, and LOD = 0.13807 ng mL-1. Sample spiking experiments were conducted, and the recovery rate of spiking was 81.19%-130.17%. A comparative analysis of differences in the current produced by multiple toxins, HT-29 cells, as well as single cells in cell populations, was performed. This method can be applied in real-time monitoring of mycotoxin toxicity during food processing in living cells and provides a novel idea for enhancing food quality and safety in a nanoenvironment.


Assuntos
Micotoxinas , Humanos , Peróxido de Hidrogênio , Micotoxinas/toxicidade , Oxirredução , Estresse Oxidativo , Espécies Reativas de Oxigênio
17.
Cell Death Dis ; 12(1): 23, 2021 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-33414461

RESUMO

Extracellular vesicles (EVs), as a novel intercellular communication carrier transferring cargo microRNAs (miRNAs), could play important roles in the brain remodeling process after ischemic stroke. However, the detailed mechanisms involved in EVs derived miRNAs-mediated cellular interactions in the brain remain unclear. Several studies indicated that microRNA-98 (miR-98) might participate in the pathogenesis of ischemic stroke. Here, we showed that expression of miR-98 in penumbra field kept up on the first day but dropped sharply on the 3rd day after ischemic stroke in rats, indicating that miR-98 could function as an endogenous protective factor post-ischemia. Overexpression of miR-98 targeted inhibiting platelet activating factor receptor-mediated microglial phagocytosis to attenuate neuronal death. Furthermore, we showed that neurons transferred miR-98 to microglia via EVs secretion after ischemic stroke, to prevent the stress-but-viable neurons from microglial phagocytosis. Therefore, we reveal that EVs derived miR-98 act as an intercellular signal mediating neurons and microglia communication during the brain remodeling after ischemic stroke. The present work provides a novel insight into the roles of EVs in the stroke pathogenesis and a new EVs-miRNAs-based therapeutic strategy for stroke.


Assuntos
Isquemia Encefálica/genética , Vesículas Extracelulares/metabolismo , MicroRNAs/metabolismo , Microglia/metabolismo , Neurônios/metabolismo , Doença Aguda , Animais , Modelos Animais de Doenças , Humanos , AVC Isquêmico , Fagocitose , Ratos
18.
Anal Bioanal Chem ; 413(4): 1127-1136, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33420534

RESUMO

Antibiotic resistance has become a serious threat to food safety and public health globally. Therefore, the development of a sensitive, quick, and simple method for antibiotic susceptibility testing is an urgent and crucial need. A novel concentration gradient microfluidic chip was designed in this work to generate antibiotic concentration gradient, culture bacteria, and produce fluorescence emission. An in-house-assembled fluorescence detection platform was constructed, and experiments were conducted to verify the linearity of the generated concentration gradient, explore the appropriate incubation time and flow rate for the microfluidic chip, and study the effect of long-term acid-based food processing on antibiotic susceptibility testing. Experimental results show that the concentration gradient generated by the microfluidic chip exhibited good linearity, stability, and controllability. The appropriate flow rate and incubation time for the microfluidic chip were 2 µL/min and 5 h, respectively. The use of this microfluidic chip for testing antibiotic resistance of Salmonella to ofloxacin and ampicillin generated results that were completely consistent with test results obtained using the gold-standard method. Furthermore, Salmonella showed greater sensitivity to antibiotics under strong acid conditions, confirming the potential influence of acid-based food processing on antibiotic susceptibility testing of real samples. The designed microfluidic chip provides a high-throughput, sensitive, and rapid antibiotic susceptibility testing method that combines the microfluidic chip and the fluorescence detection platform. The application of this method would facilitate determination of antibiotic-resistant bacterial strains for clinicians and researchers, and enable monitoring of changes in bacterial resistance during food processing.


Assuntos
Antibacterianos/farmacologia , Ensaios de Triagem em Larga Escala/instrumentação , Testes de Sensibilidade Microbiana/instrumentação , Técnicas Analíticas Microfluídicas/instrumentação , Salmonella/efeitos dos fármacos , Ampicilina/farmacologia , Farmacorresistência Bacteriana , Desenho de Equipamento , Humanos , Ofloxacino/farmacologia , Infecções por Salmonella/tratamento farmacológico , Infecções por Salmonella/microbiologia
19.
Luminescence ; 36(2): 345-352, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32945114

RESUMO

Paraquat (PQ) residue is harmful for human health, agriculture, and the aquatic environment. This paper proposes a novel fluorescent molecularly imprinted polymer (MIP), SiO2 @CdTe QDs@MIP, for PQ detection and adsorption. The MIP was synthesized using 3-aminopropyltriethoxysilane as the functional monomer, 4,4'-bipyridyl as the template molecule, and tetraethoxysilane as the cross-linker. In addition, CdTe quantum dots featuring unique optical characteristics and excellent photochemical stability were combined as signal reporter. The synthesized MIP had a Brunauer-Emmett-Teller surface area of 68.2 m2 /g, pore volume of 0.42 cm3 /g and pore size of 6.9 nm, demonstrating the potential for both PQ detection and adsorption. For PQ detection, the MIP could achieve three orders of magnitude better than the limit of detection, and one order of magnitude wider detection range than existing methods. The PQ recovery values for real samples of water and corn were 96.4-102.1% and 93.9-97.3%, respectively. The amount of PQ detected by the MIP was within 98.05% on average of that using high-performance liquid chromatography. For PQ adsorption, the MIP had an adsorption capacity of 3.36 mg/g, and followed a pseudo-second-order kinetic model with excellent toxicological characteristics. Overall, the novel SiO2 @CdTe QDs@MIP proposed in this paper could facilitate an efficient and convenient method for PQ detection and adsorption.

20.
J Hazard Mater ; 406: 124330, 2021 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-33144016

RESUMO

Antibacterial hydrogels have received attention for preventing infections and for their biomedical applications. However, traditional antibiotics-containing and metal nanoparticle-containing hydrogels often cause bacterial resistance, exhibit low biocompatibility, and lack real-time monitoring capability. Here, a fluorescent antibacterial hydrogel with antibacterial ability, excellent optical performance, and high biocompatibility was developed based on cationic carbon dots (CDs), pectin, and acrylic acid triggered construction of the hydrogel network by cross-linker. The antibacterial high-cationic CDs (+51.20 mV) were synthesized by a simple hydrothermal method and released from hydrogel in response to broken hydrogen bonds due to a change in the ambient environment caused by the growing bacteria. The hydrogel showed long-term potent broad-spectrum antibacterial ability (even drug-resistant bacteria) due to the bacterial membrane seriously damaged by the released CDs. The inhibitory capability of this hydrogel was 108.5-fold higher than the other hydrogel. After implantation or incubation with cells, no obvious cytotoxicity or tissue toxicity was observed for the antibacterial hydrogel. This hydrogel enhanced both the application of CDs in vivo and the biosafety of hydrogel. Furthermore, the multicolor fluorescence emission produced by CD provides a potential idea for the development of dual-function hydrogels with in situ monitoring and prevention of bacterial infections to treat wounds.


Assuntos
Carbono , Hidrogéis , Antibacterianos/toxicidade , Fluorescência , Cicatrização
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