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1.
Artigo em Inglês | MEDLINE | ID: mdl-34484384

RESUMO

Objective: To evaluate the effectiveness and safety of Chinese patent medicine for mild-to-moderate active ulcerative colitis (UC) using network meta-analysis (NMA). Methods: We systematically searched PubMed, Cochrane library, Embase, Sino-Med, China National Knowledge Infrastructure (CNKI), Wanfang, and Chinese Scientific Journal Database (VIP) databases to October, 2020. We included randomized controlled trials (RCTs) on Chinese patent medicine for mild-to-moderate active UC. The main analysis was complemented by network subanalyses and standard pairwise comparisons. Statistical heterogeneity, inconsistencies, and ranking probability were also evaluated. Results: The databases search identified 3222 citations, of which 33 RCTs involving 2971 patients met the inclusion criteria. A total of 15 Chinese patent medicines were analyzed. The overall quality of the included studies was low. Pairwise meta-analysis showed that Chinese patent medicine was superior to Mesalazine in improving disappearances of clinical symptoms, recurrence rate, and Mayo score. Based on decreases in adverse events, results from NMA showed that Xilei powder plus Mesalazine was more effective than other drugs. Other NMA results indicated that Danshen freeze-dried powder plus Mesalazine (RR: 0.13; 95% CI, 0.02-0.78) and Kangfuxin lotion plus Mesalazine (RR: 0.24; 95% CI, 0.07-0.57) were superior to Mesalazine in decreasing recurrence rate. Another NMA result indicated that Kangfuxin lotion plus Mesalazine (RR: 0.00; 95% CI, 0.00-0.02) and Zhi Kang capsule plus Mesalazine (RR: 0.00; 95% CI, 0.00-0.02) were superior to Mesalazine in increasing the disappearance of tenesmus. Conclusion: In the probability sorting, Xilei powder combined with Mesalazine ranked first for having the fewest adverse events, Maintaining Intestines Antidiarrheal Pills combined with Mesalazine ranked first for having the lowest recurrence rate, Xilei powder combined with Mesalazine ranked first for improving disappearance rate of mucopurulent bloody stool/abdominal pain, and Kangfuxin lotion combined with Mesalazine ranked first for improving the disappearance rate of diarrhea/tenesmus. However, there is a lack of direct comparisons among Chinese patent medicines for UC. More multiarm RCTs are needed in the future to provide direct comparative evidence.

2.
Zhongguo Zhong Yao Za Zhi ; 46(15): 3934-3942, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34472270

RESUMO

The present study aimed to explore the effect of Erxian Decoction on proteomics of osteoblasts stimulated by hydrogen peroxide(H_2O_2) and its protective mechanism with the H_2O_2-induced cell model of oxidative stress. The primary osteoblasts were cultured from the skulls of newborn rats(within 24 hours) and divided into a control group, a model group, a Fosamax group, and an Erxian Decoction group. Blank serum was added in the control group and model group, and the drug-containing serum was added correspondingly to the remaining two groups. After 45 hours, H_2O_(2 )stimulation was conducted for three hours except for the control group, followed by protein extraction. Nano-LC-LTQ-Orbitrap system was used for protein detection, Protein Discovery for protein identification, and SIEVE for quantitative and qualitative analysis. Furthermore, following the blocking of PI3 K signaling pathway by LY294002(10 µmol·L~(-1)), a control group, a model group, an LY294002 group, an Erxian Decoction group, and an Erxian Decoction + LY294002 group were set up to observe the effect of Erxian Decoction on cell proliferation, alkaline phosphatase(ALP) activity, and the relative expression of BMP-2, OPG, p-Akt, p-FoxO1 of osteoblasts stimulated by H_2O_2 under LY294002 intervention. The results revealed that 78 differential proteins were discovered between the Erxian Decoction group and model group, which were involved in the regulation of PI3 K/Akt, glucagon, estrogen, insulin, and other signaling pathways. LY294002 blunted the promoting effect of Erxian Decoction on osteoblast proliferation and significantly down-regulated the expression of OPG and p-FoxO1, whereas its down-regulation on the expression of BMP-2 and p-Akt was not significant. Both LY294002 and Erxian Decoction increased the ALP activity of osteoblasts, which may be related to the cell state and the cell differentiation. The above results suggest that Erxian Decoction can protect osteoblasts stimulated by H_2O_2, with the PI3 K/Akt signaling pathway as one of the internal mechanisms.


Assuntos
Peróxido de Hidrogênio , Fosfatidilinositol 3-Quinases , Animais , Medicamentos de Ervas Chinesas , Osteoblastos/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Proteômica , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Transdução de Sinais
3.
BMC Complement Med Ther ; 21(1): 228, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34517855

RESUMO

BACKGROUND: Traditional Chinese Medicine (TCM) has been a proposed treatment option for ulcerative colitis (UC), however it has been difficult to understand the breadth and depth of evidence as various Chinese medicine therapies may produce effects differently. The aim of this evidence mapping is to visually understand the available evidence in the use of TCM in the treatment of UC, and to identify gaps in evidence to inform priorities of future research. METHODS: A systematic electronic literature search of six databases were performed to identify systematic reviews (SRs) on different Chinese medicine therapies in the treatment in UC. Methodological quality of the included SRs was assessed using AMSTAR 2. RESULTS: The mapping was based on 73 SRs, which included nine interventions that met eligibility criteria. The quality of the included SRs was very low. The diseases stages of patients with UC varied greatly, from active to remission, to non-acute outbreak, to not reported. The results mostly favored the method of intervention. Oral administration combined with enema was the most widely used route of administration in secondary research. CONCLUSION: Based on the current evidence, the treatment of UC with TCM can only be recommended cautiously. A majority of included SRs did not report the location of the disease, the disease classification, and the route of administration of the intervention. Further research is needed on the effectiveness of Chinese medicine alone in the treatment of UC. The effectiveness of combined Chinese and conventional medicine combined with different routes of administration cannot be confirmed. Attention should be paid to the methodological quality of the systematic review. Unifies the outcome indicators used in the evaluation of effectiveness.

4.
BMC Complement Med Ther ; 20(1): 309, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33054760

RESUMO

BACKGROUND: Ulcerative colitis, characterized by diarrhea, bloody stools and abdominal pain, is a chronic, idiopathic inflammatory disease of the colonic mucosa. In recent years, the incidence of ulcerative colitis presents an increasing trend year by year. Acupuncture, as a potential effective treatment for ulcerative colitis, is widely used in clinical practice. METHODS: We searched PubMed, the Cochrane Library, Chinese CBM Database, China National Knowledge Infrastructure, Chinese VIP Information, and Wanfang Database from the date of the establishment of each database up to March, 2019. We included randomized controlled clinical trials (RCT) comparing acupuncture versus conventional conventional medicine or comparing acupuncture combined with conventional medicine versus conventional medicine in participants with ulcerative colitis. Two authors screened all references, assessed the risk of bias and extracted data independently. We summarized data using risk ratios (RR) with 95% confidence intervals (CI) for binary outcomes. We performed meta-analyses using random effects model. We assessed overall quality of evidence using GRADE. RESULTS: We included 13 RCTs (1030 participants, 515 in the acupuncture group and 515 in the control group). Only one study tested head acupuncture, and the other 12 tested body acupuncture. The treatment duration ranged from 14 to 60 days. Seven trials compared acupuncture alone versus conventional medicine, and six compared acupuncture combined with conventional medicine versus conventional medicine. Acupuncture combined with mesalazine showed better clinical effect (improved clinical symptoms, colonoscopy results and stool examination results) (RR 1.25, 95% CI 1.19 to 1.41; 232 participants; 4 trials; low quality evidence) and better colonoscopy curative effect (RR 1.33, 95% CI 1.04 to 1.71; 108 participants; 2 trials; moderate quality evidence) compared to mesalazine. Acupuncture showed better clinical effect compared to the combination of metronidazole and sulfasalazine (RR 1.21, 95%CI 1.10, 1.34; 318 participants; 3 trials; moderate quality evidence). There was no significant difference in the incidence of adverse events between groups. CONCLUSIONS: Both acupuncture alone and acupuncture combined with conventional medicine may be effective in treating ulcerative colitis compared to conventional medicine. Our findings must be interpreted with caution due to high or unclear risk of bias of the included trials.


Assuntos
Terapia por Acupuntura/métodos , Colite Ulcerativa/terapia , Terapia Combinada , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
5.
World J Gastroenterol ; 26(30): 4557-4563, 2020 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-32874064

RESUMO

BACKGROUND: At present, minimally invasive endoscopic treatment is mostly used for patients with actively bleeding Dieulafoy's lesions, , as it has the advantages of minimal trauma, short operation time and good hemostatic effect, although bleeding can easily recur postoperatively. Recently, extensive gastric cuneiform resection has been advocated for use in these patients because the constant-diameter artery follows a long path to the gastric mucosa. CASE SUMMARY: A 47-year-old man was admitted to the hospital for repeated hematemesis and black stool, and he was diagnosed with Dieulafoy's disease. We chose a method that not only simulates surgical gastric cuneiform resection but also reduces trauma. We performed enlarged local endoscopic full-thickness resection of the gastric wall and abdominal constant-diameter artery and sutured the gastric wall. Postoperative follow-up showed that the constant-diameter artery had been resected from the gastric wall, which was confirmed to have no blood flow signals by endoscopic ultrasonography. CONCLUSION: Endoscopic full-thickness resection of the gastric wall and abdominal constant-diameter artery with suturing of the gastric wall has demonstrated potential as a new treatment for Dieulafoy's disease.


Assuntos
Hemorragia Gastrointestinal , Hemostase Endoscópica , Mucosa Gástrica/diagnóstico por imagem , Mucosa Gástrica/cirurgia , Hemorragia Gastrointestinal/diagnóstico por imagem , Hemorragia Gastrointestinal/etiologia , Hemorragia Gastrointestinal/cirurgia , Gastroscopia , Humanos , Masculino , Pessoa de Meia-Idade , Estômago/diagnóstico por imagem , Estômago/cirurgia
6.
J Orthop Translat ; 24: 112-120, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32775203

RESUMO

Background/objectives: For treatment of large bone defects challenging in orthopaedic clinics, bone graft substitutes are commonly used for the majority of surgeons. It would be proposed in the current study that our bioactive scaffolds could additionally serve as a local delivery system for therapeutic small molecule agents capable of providing support to enhance biological bone repair. Methods: In this study, composite scaffolds made of poly (lactic-co-glycolic acid) (PLGA) and tricalcium phosphate (TCP) named by P/T was fabricated by a low-temperature rapid prototyping technique. For optimizing the scaffolds, the phytomolecule icaritin (ICT) was incorporated into P/T scaffolds called P/T/ICT. The osteogenic efficacies of the two groups of scaffolds were compared in a successfully established calvarial defect model in rats. Bone regeneration was evaluated by X-ray, micro-computerised tomography (micro-CT), and histology at weeks 4 and/or 8 post-implantation. In vitro induction of osteogenesis and osteoclastogenesis was established for identification of differentiation potentials evoked by icaritin in primary cultured precursor cells. Results: The results of radiographies and decalcified histology demonstrated more area and volume fractions of newly formed bone within bone defect sites implanted with P/T/ICT scaffold than that with P/T scaffold. Undecalcified histological results presented more osteoid and mineralized bone tissues, and also more active bone remodeling in P/T/ICT group than that in P/T group. The results of histological staining in osteoclast-like cells and newly formed vessels indicated favorable biocompatibility, rapid bioresorption and more new vessel growth in P/T/ICT scaffolds in contrast to P/T scaffolds. Based on in vitro induction, the results presented that icaritin could significantly facilitate osteogenic differentiation, while suppressed adipogenic differentiation. Meanwhile, icaritin demonstrated remarkable inhibition of osteoclastogenic differentiation. Conclusion: The finding that P/T/ICT composite scaffold can enhance bone regeneration in calvarial bone defects through facilitating effective bone formation and restraining excessive bone resorption. The translational potential of this article: The osteogenic bioactivity of icaritin facilitated PLGA/TCP/icartin composite scaffold to exert significant bone regeneration in calvarial defects in rat model. It might form an optimized foundation for potential clinical validation in bone defects application.

7.
ACS Synth Biol ; 8(2): 425-433, 2019 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30668109

RESUMO

Keratinases are becoming biotechnologically important since they have shown potential in hydrolysis of recalcitrant keratins with highly rigid and strongly cross-linked structures. However, the large-scale application of keratinases has been limited by the inefficient expression level and low enzyme activity. In this work, we employed pro-peptide engineering and saturation mutagenesis to construct excellent keratinase variants with improved activities. It turned out that amino acid substitutions at the pro-peptide cleavage site (P1) could accelerate the release of active mature enzymes, resulting in a 3-fold activity increase. Eighteen sites of the pro-peptide area were targeted for codon mutagenesis, and a multisite saturation mutagenesis library of the six potential sites was generated, achieving a significant improvement of keratinase activity from 179 to 1114 units/mL. Also, the mutants exhibited alterant catalytic properties. Finally, fermentation for keratinase production in a 15 L fermenter was carried out, and the enzyme activity reached up to over 3000 units/mL. Our results demonstrated that pro-peptide engineering played a crucial role in high expression and engineering of proteases. This study provides a universal route toward improvement of industrial enzymes that were first synthesized as precursors in the form of pre-pro-protein.


Assuntos
Biotecnologia/métodos , Peptídeo Hidrolases/metabolismo , Substituição de Aminoácidos , Bacillus/enzimologia , Catálise , Concentração de Íons de Hidrogênio , Mutagênese , Peptídeo Hidrolases/genética , Especificidade por Substrato
8.
Chemosphere ; 215: 25-32, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30300808

RESUMO

Biodegradable plastics, as alternatives to conventional plastics, are increasingly used, but their interactions with organic pollutants are still unknown. In this study, the sorption and desorption behaviors on a type of biodegradable plastic-poly(butylene adipate co-terephtalate) (PBAT) were investigated, and at the same time two types of conventional plastics-polyethylene (PEc and PEv) and polystyrene (PS) were used for comparison. Phenanthrene (PHEN) was chosen as one of representative organic pollutants. Results indicated that the sorption and desorption capacities of PBAT were not only higher than those of the other types of microplastics, but also higher than those of carbonaceous geosorbents. The surface area normalized results illustrated that sorption and desorption of the microplastics were positively correlated with their abundance of rubbery subfraction. The sorption kinetic results showed that the sorption rates of PBAT and PEc were higher than PEv and PS. The effects of water chemistry factors including salinity, dissolved organic matter and Cu2+ ion on the sorption process displayed the same trend, but the degrees of influence on the four microplastics differed. The degrees of influence were mainly dependent on the abundance of rubbery subfraction for microplastics. These findings indicate that the biodegradable poly(butylene adipate co-terephtalate) microplastics are actually stronger vectors than the conventional microplastics, and crystallization characteristics of the microplastics have great influences on the vector effect.


Assuntos
Fenantrenos/química , Fenantrenos/isolamento & purificação , Plásticos/química , Poliésteres/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação , Adsorção
9.
J Biomed Mater Res B Appl Biomater ; 106(5): 1934-1940, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-28960835

RESUMO

Beta-tricalcium phosphate (ß-TCP) has been widely used for bone regeneration for many years. However, there are few studies on the osteoconduction ability of different shaped ß-TCP scaffolds. In this study, we compared the osteoconductive potential between the tubular and cylindrical ß-TCP scaffolds in long bone defect animal model. The results showed that more regenerated bone and a better healing property were observed in tubular group than that in cylindrical group. By hematoxylin-eosin staining, the central part of the callus was more compacted in tubular group. And moreover, the increased osteocalcin and osterix expression were found in tubular group, suggesting more vigorous regeneration of bone defect. These results demonstrated that tubular ß-TCP scaffold would be more benefit to promote bone regeneration, indicating that tubular ß-TCP scaffold has a good potential for long bone defect repair in clinical practice. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1934-1940, 2018.


Assuntos
Materiais Biocompatíveis , Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos , Fosfatos de Cálcio , Tíbia , Tecidos Suporte/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Substitutos Ósseos/química , Substitutos Ósseos/farmacologia , Fosfatos de Cálcio/química , Fosfatos de Cálcio/farmacologia , Masculino , Coelhos , Ratos , Ratos Sprague-Dawley , Tíbia/lesões , Tíbia/metabolismo , Tíbia/patologia
10.
Oncotarget ; 8(52): 89913-89922, 2017 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-29163798

RESUMO

Distraction osteogenesis (DO), one of effective therapies for bone regeneration, has been received more attention in recent years. However, the underlying mechanism remains elusive. Recently, microRNAs (miRNAs) have been reported to play important roles in regulating osteogenesis and bone formation. We therefore provided the hypothesis that miRNAs could involve in the DO-mediated bone regeneration. After successfully established the DO model of rats, a miRNA microarray was performed to find the differently expressed miRNAs in DO and control groups in this study. As one of the most downregulated miRNAs, miR-144-3p was found to be decreased during osteogenic differentiation in mesenchymal stem cells of rats (rBMSCs) and DO model. And miR-144-3p overexpression suppressed the osteogenesis while its inhibitor promoted osteogenesis. Furthermore, Connexin-43, an essential regulator for osteogenesis, was validated to be a novel target for miR-144-3p. Finally, miR-144-3p inhibitor modified MSCs promoted mineralization of distracted bone in rat DO model. In conclusion, miR-144-3p was found to regulate osteogenesis and inhibition of miR-144-3p resulted in acceleration of mineralization of DO, which not only give clues to understanding the mechanism of DO but also provide a potential therapeutic target in clinical practice.

11.
Chemosphere ; 181: 433-439, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28458218

RESUMO

Forty-eight surface sediments were collected from three mangrove wetlands in the Pearl River Estuary (PRE) of South China to investigate the distribution of organophosphorus flame retardants (OPFRs) and the relationship between OPFRs and microbial community structure determined by phospholipid fatty acid. Concentrations of ΣOPFRs in mangrove sediments of the PRE ranged from 13.2 to 377.1 ng g-1 dry weight. Levels of ΣOPFRs in mangrove sediments from Shenzhen and Guangzhou were significantly higher than those from Zhuhai, indicating that OPFRs were linked to industrialization and urbanization. Tris(chloropropyl)phosphate was the predominant profile of OPFRs in mangrove sediments from Shenzhen (38.9%) and Guangzhou (35.0%), while the composition profile of OPFRs in mangrove sediments from Zhuhai was dominated by tris(2-chloroethyl) phosphate (25.5%). The mass inventories of OPFRs in the mangrove sediments of Guangzhou, Zhuhai and Shenzhen were 439.5, 133.5 and 662.3 ng cm-2, respectively. Redundancy analysis revealed that OPFRs induced a shift in the structure of mangrove sediment microbial community and the variations were significantly correlated with tris(1,3-dichloro-2-propyl)phosphate and tris(2-butoxyethyl) phosphate.


Assuntos
Retardadores de Chama , Áreas Alagadas , Anticorpos Fosfo-Específicos/análise , China , Estuários , Retardadores de Chama/análise , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiologia , Fenômenos Microbiológicos , Organofosfatos/análise , Compostos Organofosforados/análise
12.
Mar Pollut Bull ; 118(1-2): 413-419, 2017 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-28292528

RESUMO

Six marine biota species were collected from the Xuande Atoll, South China Sea to investigate the bioaccumulation of dichlorodiphenyltrichloroethane (DDT), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), decabromodiphenyl ethane (DBDPE) and dechlorane plus (DP). Pike conger (Muraenesox talabonoides) had the highest concentrations of halogenated organic pollutants (HOPs) among the six marine biota species. DDTs were the predominant HOPs, followed by PCBs and PBDEs, with minor contributions of DBDPE and DP. Twenty-one percent of samples had ratios of (DDE+DDD)/ΣDDTs lower than 0.5, implying the presence of fresh DDT inputs in the environment of the Xuande Atoll. The biomagnification factor values for DDTs, PCBs, PBDEs and DP were higher than 1, suggesting biomagnification of these contaminants in the marine food chains. Consumption of seafood from the Xuande Atoll might not subject local residents in the coastal areas of South China to health risks as far as HOPs are concerned.


Assuntos
Biota , Monitoramento Ambiental , Cadeia Alimentar , Poluentes Químicos da Água/análise , Animais , China , DDT/análise , Dieta , Éteres Difenil Halogenados/análise , Halogenação , Hidrocarbonetos Clorados , Bifenilos Policlorados/análise , Compostos Policíclicos , Alimentos Marinhos/análise
13.
FASEB J ; 31(3): 954-964, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-27895107

RESUMO

Tendon injures are common orthopedic conditions, but tendon development and the pathogenesis of tendon injures, such as tendinopathy, remain largely unknown and have limited the development of clinical therapy. Studies on tenogenic differentiation at the molecular level may help in developing novel therapeutic strategies. As novel regulators, long noncoding RNAs (lncRNAs) have been found to have widespread biological functions, and emerging evidence demonstrates that lncRNAs may play important regulatory roles in cell differentiation and tissue regeneration. In this study, we found that lncRNA H19 stimulated tenogenesis of human tendon-derived stem cells. Stable overexpression of H19 significantly accelerated TGF-ß1-induced tenogenic differentiation in vitro and accelerated tendon healing in a mouse tendon defect model. H19 directly targeted miR-29b-3p, which is considered to be a negative regulator of tenogenesis. Furthermore, miR-29b-3p directly suppressed the expression of TGF-ß1 and type I collagen, thereby forming a novel regulatory feedback loop between H19 and TGF-ß1 to mediate tenogenic differentiation. Our study demonstrated that H19 promotes tenogenic differentiation both in vitro and in vivo by targeting miR-29b-3p and activating TGF-ß1 signaling. Regulation of the TGF-ß1/H19/miR-29b-3p regulatory loop may be a new strategy for treating tendon injury.-Lu, Y.-F., Liu, Y., Fu, W.-M., Xu, J., Wang, B., Sun, Y.-X., Wu, T.-Y., Xu, L.-L, Chan, K.-M., Zhang, J.-F., Li, G. Long noncoding RNA H19 accelerates tenogenic differentiation and promotes tendon healing through targeting miR-29b-3p and activating TGF-ß1 signaling.


Assuntos
MicroRNAs/genética , RNA Longo não Codificante/genética , Tendões/fisiologia , Fator de Crescimento Transformador beta1/genética , Cicatrização , Linhagem Celular , Células Cultivadas , Colágeno Tipo I/genética , Colágeno Tipo I/metabolismo , Humanos , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Tendões/citologia , Tendões/metabolismo , Fator de Crescimento Transformador beta1/metabolismo
14.
Environ Sci Pollut Res Int ; 23(21): 22163-22171, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27544528

RESUMO

Heavy metals were measured by flame atomic absorption in male hair from residents in urban and rural areas in Chongqing. The median values of the Cd, Cu, Ni, Pb and Zn were 2.90, 23.9, 9.31, 39.3 and 203 µg/g in urban areas and 0.84, 13.4, 5.56, 14.5 and 169 µg/g in rural area, respectively. The levels of Cd, Ni and Pb both in urban and rural areas lie at the high end of the worldwide figures. The differences in heavy metal distribution pattern indicated that there were more sources of Cd and Pb in urban areas. The levels of Cd were increasing along with the growth of age except for the aged people in urban areas, and no significant relationship was observed between the levels of the heavy metal and the age. It is noticed that the hair of smokers exhibited more heavy metal levels than that of non-smokers both in urban and rural areas. In addition, the hair metal levels of the smokers and non-smokers in urban areas were significantly higher than those in rural area, respectively. Significant pairwise correlations (p < 0.01) were observed among Cd, Cu, Ni and Pb in rural area and only between Cu and Ni and between Pb and Ni in urban areas, indicating the elements in these two areas might originate from different sources. The elevated levels of Cd, Pb and Ni implied that the residents both in urban and rural areas might be at high risk of toxic metal exposure, especially for the children.


Assuntos
Cabelo/química , Metais Pesados/metabolismo , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Pré-Escolar , China , Monitoramento Ambiental , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Medição de Risco , População Rural , Fumar , Distribuição Tecidual , População Urbana , Adulto Jovem
15.
Hum Mov Sci ; 49: 196-205, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27428595

RESUMO

In the present study we investigated the effects of different durations of using high-heeled shoes on plantar pressure and gait. A questionnaire survey and dynamic plantar pressure measurements were performed in 20 control females and 117 females who had worn high-heeled shoes for a long time. According to the duration of using high-heeled shoes (as specified in the questionnaire), subjects were divided into a control group and five groups with different durations of use (i.e. <2years, 2-5years, 6-10years, 11-20years and >20years). Parameters, including peak pressure, impulse and pressure duration, in different plantar regions were measured with the Footscan pressure plate. The 2-5years group had smaller midfoot contact areas for both feet and higher subtalar joint mobility, while the 6-10years group had larger midfoot contact areas for both feet and prolonged foot flat phase during gait. The peak pressure and impulse under the second and fourth metatarsus were increased with the prolonged wearing of high-heeled shoes, and the pressure and impulse under the midfoot were substantially reduced in the 2-5years group. The findings suggest that long-term use of high-heeled shoes can induce changes in arch morphology: the longitudinal arch tends to be elevated within 2-5years; the longitudinal arch tends to be flattened within 6-10years; and the forefoot latitudinal arch tends to collapse in more than 20years.


Assuntos
Fenômenos Biomecânicos/fisiologia , Antepé Humano/fisiopatologia , Marcha/fisiologia , Placa Plantar/fisiopatologia , Sapatos , Suporte de Carga/fisiologia , Adulto , China , Feminino , Pé Chato/fisiopatologia , Seguimentos , Humanos , Pessoa de Meia-Idade , Adulto Jovem
16.
Arch Environ Contam Toxicol ; 71(3): 359-64, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27421724

RESUMO

Thirty surface sediments and three sediment cores were collected from mangrove wetlands in the Pearl River Estuary of South China to investigate the spatial and vertical distribution of Dechlorane Plus (DP). DP concentrations in the mangrove surface sediments ranged from 0.0130 to 1.504 ng/g dry weight (dw). DP concentrations in sediments from Shenzhen were significantly greater than those from Guangzhou and Zhuhai. Anti-Cl11-DP, the dechlorinated product of anti-DP, was also detected in the mangrove sediments with concentrations ranged from not detected to 0.0198 ng/g dw. Significant positive relationship between anti-Cl11-DP and anti-DP levels was observed in the mangrove sediments, suggesting that photo and/or microbial degradation of anti-DP might occur in the sediments. The f anti values in the mangrove sediments were close to those in the technical DP products, suggesting that stereoselective enrichment of anti-DP may not exist in the mangrove sediments. DP concentrations in the mangrove sediment cores generally showed an increasing trend from the bottom to top layers. This is the first study to report the occurrence of DP and its degradation product in the mangrove wetlands.


Assuntos
Monitoramento Ambiental , Sedimentos Geológicos/química , Hidrocarbonetos Clorados/análise , Compostos Policíclicos/análise , Poluentes Químicos da Água/análise , Áreas Alagadas , China
17.
J Hazard Mater ; 316: 186-93, 2016 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-27235826

RESUMO

The applicability of bentonite-supported nanoscale zero-valent iron (B-nZVI) as a catalyst to activate persulfate (PS) for the simultaneous removal of Cr(VI) and phenol was systematically investigated in this study. Experimental results demonstrated that phenol degradation was significantly enhanced under acidic condition and with oxygen supply, whereas the Cr(VI) reduction was not obviously declined. The removal efficiencies of Cr(VI) and phenol in B-nZVI/PS combined system were 99.8 and 72.3%, respectively. Cr(VI) reduction and phenol oxidation was simultaneously achieved in B-nZVI/PS combined system, which provided a promising environmental treatment for industrial wastewater containing metal ions and organic compounds. An acidic condition was more favorable to the decomposition of persulfate for the production of sulfate radicals. Radical scavenging tests revealed that the predominant reactive oxygen species for phenol degradation was SO4(-), neither HO nor O2(-). A reaction mechanism, which involves the Cr(VI) removal mainly by the reduction of nZVI and the degradation of phenol mainly by the SO4(-) from the decomposition of persulfate, was proposed. These findings revealed that B-nZVI/PS combined system has a potential in the environmental remediation polluted jointly by organic compounds and/or heavy metals.

18.
Sci Rep ; 6: 20121, 2016 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-26853553

RESUMO

Bone homeostasis is tightly orchestrated and maintained by the balance between osteoblasts and osteoclasts. Recent studies have greatly expanded our understanding of the molecular mechanisms of cellular differentiation. However, the functional roles of non-coding RNAs particularly lncRNAs in remodeling bone architecture remain elusive. In our study, lncRNA H19 was found to be upregulated during osteogenesis in hMSCs. Stable expression of H19 significantly accelerated in vivo and in vitro osteoblast differentiation. Meanwhile, by using bioinformatic investigations and RIP assays combined with luciferase reporter assays, we demonstrated that H19 functioned as an miRNA sponge for miR-141 and miR-22, both of which were negative regulators of osteogenesis and Wnt/ß-catenin pathway. Further investigations revealed that H19 antagonized the functions of these two miRNAs and led to de-repression of their shared target gene ß-catenin, which eventually activated Wnt/ß-catenin pathway and hence potentiated osteogenesis. In addition, we also identified a novel regulatory feedback loop between H19 and its encoded miR-675-5p. And miR-675-5p was found to directly target H19 and counteracted osteoblast differentiation. To sum up, these observations indicate that the lncRNA H19 modulates Wnt/ß-catenin pathway by acting as a competing endogenous RNA, which may shed light on the functional role of lncRNAs in coordinating osteogenesis.


Assuntos
MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Proteínas Wnt/metabolismo , Via de Sinalização Wnt , Regiões 3' não Traduzidas , Fosfatase Alcalina/genética , Fosfatase Alcalina/metabolismo , Animais , Sequência de Bases , Western Blotting , Diferenciação Celular , Linhagem Celular , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Genes Reporter , Humanos , Imunoprecipitação , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Osteoblastos/citologia , Osteoblastos/metabolismo , Osteogênese , RNA Longo não Codificante/genética , Reação em Cadeia da Polimerase em Tempo Real , Alinhamento de Sequência , Proteínas Wnt/antagonistas & inibidores , Proteínas Wnt/genética , beta Catenina/antagonistas & inibidores , beta Catenina/genética , beta Catenina/metabolismo
19.
Mar Pollut Bull ; 99(1-2): 150-6, 2015 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-26209127

RESUMO

Four biota species were collected from mangrove ecosystems of the Pearl River Estuary to investigate the bioaccumulation and biomagnification of polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT), polybrominated diphenyl ethers (PBDEs), dechlorane plus (DP), and decabromodiphenyl ethane (DBDPE). Concentrations of ΣPCBs, ΣDDTs, ΣPBDEs, DP, DBDPE and anti-Cl11-DP (the dechlorination product of anti-DP) in mangrove biota ranged from 32.1-466, 153-3819, 3.88-59.8, 0.18-6.88, not detected (nd)-30.6 and nd-2.65 ng/g lipid weight, respectively. Daggertooth pike conger (Muraenesox cinereus) had higher concentrations of contaminants than the other three biota species. Significant positive relationship between anti-Cl11-DP and anti-DP levels was observed in mangrove biota. DDTs were the predominant HOPs in all biota species, followed by PCBs and PBDEs. All the target compounds exhibited biomagnification, with biomagnification factors greater than 1 in the studied feeding relationships. Food web magnification was found for ΣPCBs, ΣDDTs, ΣPBDEs and DP, with trophic magnification factors of 2.76, 2.61, 2.20 and 2.31, respectively.


Assuntos
Cadeia Alimentar , Éteres Difenil Halogenados/farmacocinética , Bifenilos Policlorados/farmacocinética , Poluentes Químicos da Água/farmacocinética , Animais , Biota , China , DDT/análise , DDT/farmacocinética , Ecossistema , Monitoramento Ambiental/métodos , Estuários , Peixes , Éteres Difenil Halogenados/análise , Bifenilos Policlorados/análise , Rios , Poluentes Químicos da Água/análise
20.
Ecotoxicology ; 24(7-8): 1593-600, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25956984

RESUMO

Fish are often used as good bioindicators to monitor the occurrence of persistent organic pollutants (POPs) on different scales in recent years. Forty-five golden threads (Nemipterus virgatus) were collected from six sampling sites in the northern South China Sea (SCS) to investigate the geographical distribution of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane and its metabolites (DDTs). Concentrations of PBDEs, PCBs, and DDTs ranged from 1.3-36.0, 2.3-76.5, 8.3-228 ng/g lipid weight, respectively. The highest PBDEs and DDTs concentrations were found in golden threads from Shantou, owing to the intensive electronic waste recycling activities and rapid development of agriculture. Samples from Haikou had the highest levels of PCBs, probably due to the existence of many shipbuilding yards in the past years. The concentrations of PBDEs and PCBs were found in a decreasing trend from east to west and from north to south, while DDTs concentrations had no obvious trend in the distribution. PCBs were the most prevalent contaminants in Xiamen and Yangjiang, while DDTs were the dominant compounds at the other four sampling sites. Different profiles of POPs at each sampling site may attribute to different pollution sources in the northern SCS. Ratios of (DDD + DDE)/DDTs in golden threads suggested the probability of fresh input of DDT in the northern SCS. The estimated daily intakes of PBDEs, PCBs and DDTs were 0.030-0.069, 0.167-0.258 and 0.105-1.88 ng/kg/day, respectively, which were significantly lower than the acceptable daily intake, suggesting that consumption of golden threads from the northern SCS would not subject the residents in the coastal areas of SCS to significant health risk.


Assuntos
Monitoramento Ambiental , Contaminação de Alimentos/análise , Perciformes/metabolismo , Alimentos Marinhos/análise , Poluentes Químicos da Água/análise , Animais , China , DDT/análise , Exposição Ambiental , Éteres Difenil Halogenados/análise , Humanos , Bifenilos Policlorados/análise , Medição de Risco
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