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1.
Med Phys ; 2021 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-33583029

RESUMO

PURPOSE: Traditional registration of functional magnetic resonance images (fMRI) is typically achieved through registering their co-registered structural MRI. However, it cannot achieve accurate performance in that functional units which are not necessarily located relative to anatomical structures. In addition, registration methods based on functional information focus on gray matter (GM) information but ignore the importance of white matter (WM). To overcome the limitations of exiting techniques, in this paper, we aim to register resting-state fMRI (rs-fMRI) based directly on rs-fMRI data and make full use of GM and WM information to improve the registration performance. METHODS: We provide a robust representation of WM functional connectivity features using tissue-specific patch-based functional correlation tensors (ts-PFCTs) as auxiliary information to assist registration. Furthermore, we propose a semi-supervised deep learning model that uses GM and WM information (GM ts-PFCTs and WM ts-PFCTs) during training as a fine tweak to improve registration accuracy when such information is not provided in new test image pairs. We implement our method on the 1000 Functional Connectomes Project dataset. To evaluate our method, a group-level analysis was implemented in resting-state brain functional networks after registration, resulting in t maps. RESULTS: Our method increases the peak t values of the t maps of default mode network, visual network, central executive network, and sensorimotor network to 21.4, 20.0, 18.4, and 19.0, respectively. Through comparison with traditional methods (FMRIB Software Library(FSL), Statistical Parametric Mapping _ Echo Planar Image(SPM_EPI), and SPM_T1), our method achieves an average improvement of 67.39%, 12.96%, and 25.14%. CONCLUSION: We propose a semi-supervised deep learning network by adding GM and WM information as auxiliary information for resting-state fMRI registration. GM and WM information is extracted and described as GM ts-PFCTs and WM ts-PFCTs. Experimental results show that our method achieves superior registration performance.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33448913

RESUMO

Electronic waste is the fastest growing waste stream and one of the most significant constituents is electronic plastics. In this study, the combustion kinetic of typical electronic plastic waste⸺TV plastic shell⸺was investigated using two basic kinetic methods. The reaction mechanism and kinetic compensation effect were probed as well. The thermogravimetric analysis (TGA) revealed that its degradation process can be divided into four stages, reaction initiation stage (20-300 °C), major reaction stage (300-450 °C), minor reaction stage (450-600 °C) and reaction cessation stage (600-1000 °C). The activation energy (E) were calculated and indicated that, the kinetic parameters from six model-free methods gradually decreased with α increasing from 0.1 to 0.35, and then slightly increased. The Flynn-Wall-Ozawa (FWO) method was more reliable and E values decreased from 155.0 to 147.51 kJ/mol with α range of 0.1-0.35, then gradually increased to 165.21 kJ/mol. Among the Coats-Redfern method, the first-order (F1) model had higher coefficient of determination (R2) and comparable E values with that from FWO method. The result of kinetic compensation effect confirmed that the compensation effect existed between E and A during the plastic waste combustion. A linear relationship lnA=0.183E-3.11 (R2=0.991) was obtained. The pre-exponential factors (A) were also determined as 7.67×101° min-1 based on the F1 reaction model and FWO method. Implication Statement Municipal solid waste (MSW) is a complex mixture of different components and the plastic takes up a significant portion in total MSW. Understanding the combustion process of typical electronic plastic waste, and further probing its combustion kinetic are significant. Through this study, it will be significant for the reactor designing and optimizing in practice.

3.
Arthroscopy ; 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33278528

RESUMO

PURPOSE: The purpose of the study is to determine if patients with coexisting lumbar back pain experience back pain improvement after undergoing hip arthroscopy for femoroacetabular impingement (FAI). METHODS: An IRB approved retrospective chart review compared patients undergoing hip arthroscopy for FAI with lumbar spine back pain to patients solely reporting hip pain. The modified Harris Hip Score (MHHS) and Nonarthritic Hip Score (NAHS) were recorded preoperatively and at 1-year follow up . The Oswestry disability score, which quantifies disability from lower back pain, and visual analog scale (VAS) were recorded from the Hip-Spine (HS) cohort alone. Statistical analysis was performed using paired sample t-tests with p≤ 0.05 considered significant. RESULTS: Sixty-eight patients who underwent hip arthroscopy between November 2016 and October 2018 were enrolled. Thirty-four patients with a mean age of 48.2 ± 14.0 years and BMI of 26.6 ± 6.6 had a history of back pain and 34 patients were age- and sex-matched for the Matched-Control (MC) cohort. The MC cohort had lower MHHS and NAHS scores preoperatively. The MC cohort reported a larger increase in the MHHS (p= 0.01) and NAHS scores (p= 0.01) postoperatively. More patients in the MC cohort reached MCID with MMHS (p=0.003) and NAHS (p=0.06). Following surgery, the HS cohort reported a lower Oswestry Disability score, indicating minimal disability (p= 0.01). CONCLUSION: Surgical intervention for FAI can lead to improvements in hip and back pain in patients with co-existing lumbar pathology.

4.
Huan Jing Ke Xue ; 41(10): 4446-4454, 2020 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124376

RESUMO

Presently, there are a few studies on the measurement of industrial organic solvents in China. To determine the content and species of volatile organic compounds (VOCs) in industrial organic solvents and to provide the emission factors of organic solvents, the Chinese wood and automotive coatings, which accounted for the largest proportion of production in the world, were investigated. Coating samples were obtained by sampling from companies and buying from markets, and were measured in accordance with the domestic standard testing methods for coatings. The content and composition spectrum of VOCs in the coatings were determined, and the ozone formation potential (OFP) was then calculated. The results showed that for wood coatings, the average content of the VOCs in solvent-based, water-based, and ultra-violet (UV) coatings were 37.28%, 9.88%, and 18.02%, respectively. For automotive coatings, the average content of the VOCs in water-based original equipment manufacturer (OEM) coating, solvent-based OEM coating, water-based refinishing coating, and solvent-based refinishing coating were 15.06%, 59.90%, 11.79%, and 54.50%, respectively. The content of the VOCs in different types of coatings varied substantially. The main components and OFP contributors were alcohols and ethers for water-based coatings, benzene series and esters for solvent-based coatings, and esters, alcohols, and ethers for UV coatings. The average value of the coating samples could meet the current mandatory national standards, but 12% of the solvent-based wood coating samples and 42% of the solvent-based OEM coating samples did not meet the standards. In addition, except for the benzene series of the water-based wood coatings, the contents of harmful substances in other coating samples were up to the standards.


Assuntos
Poluentes Atmosféricos , Compostos Orgânicos Voláteis , Poluentes Atmosféricos/análise , China , Monitoramento Ambiental , Compostos Orgânicos Voláteis/análise , Madeira/química
5.
Opt Express ; 28(21): 32226-32238, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33115184

RESUMO

Metasurface-based beam splitters with high efficiency, large split angle, wide bandwidth and easy fabrication are highly desirable and still in pursuit. In this paper, we propose a heuristic scheme for designing an ultra-broadband high-efficiency power beam splitter based on a homogeneous metasurface. The conversion efficiency and total transmission intensity of the power splitter stays higher than 95% and 0.66 within the wavelength region from 604 nm to 738 nm, respectively. Particularly, the conversion efficiency can maintain greater than 99% in 58 nm bandwidth. The angle between two split beams can reach a maximum of 157.82° at the wavelength of 738 nm. In addition to simplified design and easy fabrication, the proposed power beam splitter possesses high robustness as well. We expect that our design can pave a new way for realizing high-performance metasurface-based beam splitters.

6.
Opt Lett ; 45(17): 4847-4850, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32870873

RESUMO

Due to the utilization of overlapped dipole resonances, traditional Huygens' metasurfaces suffer from dipole interactions. In this Letter, we propose a design of phase-gradient Huygens' metasurface based on the quadrupole resonances excited in the cross-shaped structures. The quadrupole resonances are theoretically shown insensitive to the quadrupole interactions. Benefiting from this intrinsic property, the proposed metasurface can well suppress element interaction influence and exhibits some impressive properties, including the ability to suppress high diffraction orders, tunable anomalous refractive angles, and high transmission efficiency. The numerical results show promising potential for quadrupole resonances to be applied in advanced Huygens' metasurface designs.

7.
Front Cell Dev Biol ; 8: 349, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32478078

RESUMO

Perilipin1 (PLIN1), the most abundant lipid droplet (LD)-associated protein, plays a vital role in regulating lipid storage and breakdown in adipocytes. Recently, we found that the overexpression of PLIN1 promotes chicken preadipocyte lipid accumulation. However, the mechanisms by which transcription of the chicken PLIN1 gene is regulated remain unknown. In this study, we investigated the role of retinoid X receptor α (RXRα) in transcription of the chicken PLIN1 gene. Notably, reporter gene and expression assays showed that RXRα activates transcription of the chicken PLIN1 gene in a PPARγ-independent manner. Furthermore, promoter deletion and electrophoretic mobility shift assay (EMSA) analysis revealed that the chicken PLIN1 gene promoter region (-774/-785) contains an RXRα-binding site. Further study demonstrated that RXRα overexpression promotes differentiation of an immortalized chicken preadipocyte cell line (ICP1), causing a concomitant increase in PLIN1 transcripts. Taken together, our results show for the first time that RXRα activates transcription of the chicken PLIN1 gene in a PPARγ-independent manner, which might be at least in part responsible for RXRα-induced adipogenesis.

8.
Materials (Basel) ; 13(4)2020 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-32098307

RESUMO

The tribological properties of Ni3Si alloy were studied at high temperatures. The effect of the addition of Ti was also analyzed. The surface composition was analyzed by Raman spectroscopy. The results showed that the friction coefficient decreased with the increasing temperature, and the wear rate changed slightly from 25 to 400 °C. However, the wear resistance of the alloys decreased sharply at 600 °C, and this was due to the decrease of the high-temperature strength and the severe oxidation of the alloys. Although the oxidation resistance of Ni3Si alloy decreased with Ti addition, the tribological property was improved by the addition of Ti. The Ni3Si alloy with 5% Ti addition had the best wear resistance at high temperatures as compared to pure Ni3Si alloy and with 10% Ti addition, and the wear rates of the alloys were in the order of magnitude of 10-5 mm3/Nm. With the increase of temperature, the wear mechanism of pure Ni3Si alloy transformed from abrasive wear to oxidation wear. As the Ti content increased, the wear mechanisms of the alloys changed from abrasive wear to fatigue wear at low temperature, and oxidation wear and fatigue wear at high temperature.

9.
J Hazard Mater ; 392: 122298, 2020 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-32105956

RESUMO

The porous carbon materials formed from biomass precursors are promising candidates for adsorbing organic vapor pollutants. However, these materials have insufficient pores, which hinder their accessibility to adsorbates. This study develops an ultrahigh-surface-area porous carbon adsorbent with interlacing micro-mesoporous structures through Trichoderma viride decomposition. An orthogonal experiment is conducted, and the most suitable conditions for fabricating porous carbon with an ultrahigh SBET of 3714 m2.g-1 and a hierarchical porous structure are identified. This work achieves one of the highest specific surface areas of biomass carbons among recent studies. T. viride corrodes the internal and external microstructures of rice husks, and regulates the lignin, cellulose, and hemicellulose contents, which improve the efficiency of carbonization and chemical activation. The carbonaceous materials with microbial pretreatment exhibit better toluene adsorption performances (100 ppm: 708 mg.g-1), adsorption rates, and cyclic utilization than those without pretreatment (100 pm: 538 mg.g-1). In addition, grand canonical Monte Carlo simulation is conducted. The micropores and mesopores created after microbial pretreatment are effective toluene adsorption sites. Moreover, the diffusion coefficient calculated by utilizing Thomas model and Chemical diffusion verify that the mesopores accelerate the kinetic process of toluene adsorption.

10.
J Hazard Mater ; 384: 121276, 2020 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-31600693

RESUMO

This work reported a facile method for fabricating multi-layered polydopamine (PDA) encapsulated AuAg@C@AuAg core/shell nanosphere with a hollow interior. During the synthetic process, the preliminary Ag@C nanosphere is easily covered by an AuAg/PDA hybrid layer through the in situ redox-oxidized polymerization to form the Ag-AuAg@C@AuAg/PDA precursor, in which the AuAg bimetallic nanocrystals are simultaneously obtained via the electrochemical substitution reaction. After etching the residue Ag core, the final AuAg@C@AuAg/PDA hybrid nanosphere is achieved and the inner AuAg shows a unique nanoframe-like nanostructure. The carbon shell plays an important role for the formation and structure evolution of the AuAg@C@AuAg/PDA, and the composition can be modulated by varying the polymerization process. Owing to the well distributed AuAg nanocrystals and inner AuAg nanoframes, the AuAg@C@AuAg/PDA shows better performance than Ag-AuAg@C@AuAg/PDA precursor in catalyzing 4-nitrophenol, and the rate constant (K) to catalyst weight ratio reaches as high as 3.63 min-1 •mg-1. As a result, this work not only offers a hybrid bi-metallic nanocatalyst with excellent performance, but also has valuable implications for compositional modulation of hollow interior multi-layered nanostructure in adsorption, drug delivery, and nanocatalysis.

11.
Arch Anim Breed ; 62(2): 375-382, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31807648

RESUMO

Evidence suggests that Perilipin-1 (PLIN1) is subject to functional regulation by epigenetic modifications in women with obesity. However, whether chicken PLIN1 expression is regulated by DNA methylation is unknown. Here, Sequenom MassARRAY and real-time polymerase chain reaction (PCR) were conducted to analyze the promoter methylation status and expression of the PLIN1 gene in Northeast Agricultural University broiler lines divergently selected for abdominal fat content. We found that chicken PLIN1 expression was significantly higher in adipose tissue of fat-line broilers than in lean lines at 1-7 weeks of age, and was significantly positively correlated with abdominal fat percentage (AFP) in chicken adipose development (Pearson's r = 0.627 , P < 0.001 ). The region analyzed for DNA methylation was from - 12 to - 520  bp upstream of the translation start codon ATG, and had five CpG sites, where only the DNA methylation levels of CpG5 located at position - 490  bp were significantly higher in lean compared to fat chickens at 5 and 6 weeks ( P < 0.05 ) and were significantly negatively correlated with PLIN1 mRNA levels and AFP ( P < 0.05 ). These results shed new light on the regulation of hypertrophic growth in chicken adipose development.

12.
Front Chem ; 7: 761, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31781543

RESUMO

N-doped mesoporous carbons, NMCs, have attracted intensive attention recently and have shown potential applications in various scientific fields including catalysis and energy conversion/storage. Via modification with foreign N elements and construction of mesoporous structures for NMCs, their electronic and spin structure, as well as their porosity can be greatly tailored. And the resultant electron-donor property, surface wettability, conductivity, ion/molecular transfer and reactivity are changed accordingly. In this review, we will summarize the recent research progress of these metal-free NMCs, with an emphasis on their synthesis and performance, especially for their synthetic strategy and catalytic properties toward oxygen and nitro compound reductions, as well as their electrochemical properties as electrode materials for lithium-ion/sulfur batteries and supercapacitors. We hope for future developments, such as controlling doping methods more precisely, generating more active sites by N-doping, and finding wider applications of NMCs in other fields.

13.
J Agric Food Chem ; 67(35): 9727-9737, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31398034

RESUMO

The present study aimed to search for chicken abdominal fat deposition-related polymorphisms within RB1 and to provide functional evidence for significantly associated genetic variants. Association analyses showed that 11 single nucleotide polymorphisms (SNPs) in intron 17 of RB1, were significantly associated with both abdominal fat weight (P < 0.05) and abdominal fat percentage (P < 0.05). Functional analysis revealed that the A allele of g.32828A>G repressed the transcriptional efficiency of RB1 in vitro, through binding nuclear factor-kappa B (NF-KB) and SRY-related HMG box protein 2 (SOX2). Furthermore, RB1 mRNA expression levels in the abdominal fat tissue of individuals with the A/A genotype of g.32828A>G were lower than those of individuals with the G/G genotype. Collectively, we propose that the intronic SNP g.32828A>G of RB1 is an obesity-associated variant that directly affects binding with NF-KB and SOX2, leading to changes in RB1 expression which in turn may influence chicken abdominal fat deposition.


Assuntos
Adiposidade , Proteínas Aviárias/metabolismo , Galinhas/metabolismo , NF-kappa B/metabolismo , Proteína do Retinoblastoma/genética , Proteína do Retinoblastoma/metabolismo , Fatores de Transcrição SOX/metabolismo , Gordura Abdominal/metabolismo , Alelos , Animais , Proteínas Aviárias/genética , Sítios de Ligação , Galinhas/genética , Galinhas/crescimento & desenvolvimento , Íntrons , NF-kappa B/genética , Polimorfismo de Nucleotídeo Único , Ligação Proteica , Fatores de Transcrição SOX/genética
14.
IEEE Trans Med Imaging ; 38(10): 2352-2363, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-30908198

RESUMO

Conducting an accurate motion correction of liver dynamic contrast-enhanced magnetic resonance (DCE-MR) imaging remains challenging because of intensity variations caused by contrast agents. Such variations lead to the failure of the traditional intensity-based registration method. To address this problem, we propose a correlation-weighted sparse representation framework to separate the contrast agent from original liver DCE-MR images. This framework allows the robust registration of motion components over time without intensity variances. Existing sparse coding techniques recover a 3D image containing only contrast agents (named contrast enhancement component) from a manually labeled dictionary, whose column has the same size with the original 3D volume (3D-t mode). The high dimension of the recovery target (3D volume) and the indistinguishability between the unenhanced and enhanced images make accurate coding difficult. In this paper, we predefine an ideal time-intensity curve containing only contrast agents (named contrast agent curve) and recover it from the transpose dictionary (t-3D mode), whose column has been updated into the original time-intensity curves. The low dimension of the target (1D curve) and the significant intergroup difference between contrast agent curves and non-contrast agent curves can estimate a series of pure contrast agent curves. A "correlation-weighted" constraint is introduced for the selection of a coding subset with more contrast agent curves, leading to an efficient and accurate sparse recovery process. Then, the contrast enhancement component can be estimated by the solved sparse coefficients' map and the ideal curve and subtracted from the original DCE-MRI. Finally, we register the de-enhanced images and apply the obtained deformation fields for the original DCE-MRI to achieve the goal of motion correction. We conduct the experiments on both simulated and real liver DCE-MRI data. Compared with other state-of-the-art DCE-MRI registration methods, the experimental results show that our method achieves a better registration performance with less computational efficiency.


Assuntos
Imageamento Tridimensional/métodos , Fígado/diagnóstico por imagem , Imagem por Ressonância Magnética/métodos , Algoritmos , Meios de Contraste , Humanos
15.
Chem Biol Interact ; 300: 27-34, 2019 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-30629952

RESUMO

Selenium deficiency and T-2 toxin exposure may contribute to the development of Keshan disease characterized by congestive cardiomyopathy. The aim of this study was to explore the role of autophagy in the aggravation of selenium deficiency on T-2 toxin-induced damages on primary cardiomyocyte. Our present study demonstrated that 0.25-1 µM T-2 toxin damaged primary cardiomyocytes and selenium deficiency exacerbated T-2 toxin-induced damages by measuring the levels of MTT, lactate dehydrogenase and cleaved-caspase 3. T-2 toxin triggered autophagy in primary cardiomyocytes, as indicated by markedly increased expressions of LC3-Ⅱ and Beclin-1 mRNA levels. Rapamycin (autophagy agonist) treatment increased autophagy levels and decreased the cytotoxicity caused by T-2 toxin while 3-methyladenine (autophagy inhibitor) treatment reduced autophagy levels and enhanced the cytotoxicity of T-2 toxin, suggesting that autophagy protect primary cardiomyocytes from the cytotoxicity of T-2 toxin. Selenium deficiency lowered cytoprotective autophagy in the primary cardiomyocytes treated by T-2 toxin. It can be concluded that autophagy induced by T-2 toxin plays a role in protecting primary cardiomyocyte, but selenium deficiency decreases the protective autophagy and then exacerbate T-2 toxin-induced damages. Our finding may partly interpret the combination effects of selenium deficiency and T-2 toxin on the development of Keshan disease.


Assuntos
Autofagia/efeitos dos fármacos , Selênio/deficiência , Toxina T-2/farmacologia , Adenina/análogos & derivados , Adenina/farmacologia , Animais , Proteína Beclina-1/genética , Proteína Beclina-1/metabolismo , Caspase 3/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/metabolismo , Miócitos Cardíacos/citologia , Miócitos Cardíacos/metabolismo , Ratos , Ratos Wistar , Sirolimo/farmacologia
16.
J Poult Sci ; 56(3): 177-185, 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-32055212

RESUMO

The effects of insulin-like growth factor binding protein 2 (IGFBP2) on the expression of fatty acid synthesis regulators and triglyceride production were investigated in primary cultured chicken hepatocytes. The full-length chicken IGFBP2 coding region was synthesized by overlap extension PCR and cloned into the pcDNA3.1 vector. An in situ digestion method was used to prepare the chicken hepatocytes. Primary chicken hepatocytes were maintained in monolayer culture. Real-time PCR was used to detect changes in the expression of IGFBP2, PPARG, IGF1, IGF1R, APOAI, and LFABP, after the overexpression of IGFBP2 in chicken hepatocytes. Triglyceride production and glucose content were also evaluated using triglyceride and glucose analysis methods. The expression level of IGFBP2 increased after transfection of the IGFBP2-containing vector. The expression levels of PPARG, IGF1, and IGF1R also increased in cultured chicken hepatocytes after the overexpression of IGFBP2, whereas the expression of LFABP and APOAI decreased. Triglyceride production in primary cultured chicken hepatocytes increased after the overexpression of IGFBP2. These results suggest that IGFBP2 is involved in lipogenesis, increasing both the expression of fatty acid synthesis regulators, and triglyceride production in primary cultured chicken hepatocytes.

17.
Artigo em Inglês | MEDLINE | ID: mdl-30580104

RESUMO

Clostera restitura Walker (Lepidoptera: Notodontidae) is one of the most destructive defoliators of poplars in China. We constructed an antennal transcriptome using Illumina Hiseq 2500™ sequencing and characterized the expression profiles of odorant binding proteins for better understanding of the olfactory receptive system and the role of putative olfactory proteins in C. restitura. A total of 165 transcripts were identified, including 43 transcripts encoding putative odorant-binding proteins (OBPs), 13 chemosensory proteins (CSPs), 78 odorant receptors (ORs), 15 ionotropic receptors (IRs), 13 gustatory receptors (GRs), and 3 sensory neuron membrane proteins (SNMPs). Furthermore, we systematically analyzed expression patterns of eight OBPs from different tissues of both C. restitura sexes by using reverse transcription PCR and quantitative real time PCR (RT-qPCR). The expression level of CresGOBP2 in female antennae was approximately two times higher than in males, and two pheromone binding proteins PBPs (CresPBP1 and -PBP3) and three OBPs (CresOBP9, -10, and -16) were more highly enriched in male antennae than in female antennae. CresOBP10 showed a remarkably high expression in legs compared to other studied insects. Our results suggested that these proteins might play a key role in foraging, seeking mates, and host recognition in C. restitura. Our findings provided a foundation for future studies on the molecular mechanisms controlling the olfactory system in C. restitura and potential novel targets for pest control strategies.


Assuntos
Antenas de Artrópodes/metabolismo , Perfilação da Expressão Gênica , Genes de Insetos , Lepidópteros/metabolismo , Receptores Odorantes/metabolismo , Animais , Feminino , Ontologia Genética , Lepidópteros/classificação , Lepidópteros/genética , Masculino , Filogenia , Receptores Odorantes/genética
18.
J Agric Food Chem ; 67(2): 735-745, 2019 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-30586993

RESUMO

Our previous study reported that aflatoxin B1 (AFB1) promoted influenza replication. Mannan oligosaccharide (MOS), derived from the cell walls of yeast, is a potent immunomodulator. Here, we investigated the role of MOS in AFB1-promoted influenza replication and further explored the underlying mechanisms. In vitro and in vivo, the exposure to AFB1 alone resulted in significantly decreased weight gain and increased viral replication as well as lung and spleen damages. Increased influenza replication coupled with increases in toll-like receptor 4 (TLR4), phosphorylated nuclear factor κB, and tumor necrosis factor α (TNF-α) levels. However, MOS given in conjunction with exposure to AFB1 significantly reversed these above changes. A further study indicated that MOS activity was abolished by TLR4 knockout or TLR4 overexpression. Surprisingly, TNF-α played no role in the MOS-mediated protective effects. Collectively, our data suggest that MOS alleviates the AFB1-promoted influenza replication, inflammation, and tissue damages in a TLR4-dependent manner.


Assuntos
Aflatoxina B1/administração & dosagem , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana/metabolismo , Mananas/administração & dosagem , Oligossacarídeos/administração & dosagem , Substâncias Protetoras/administração & dosagem , Receptor 4 Toll-Like/metabolismo , Replicação Viral/efeitos dos fármacos , Animais , Humanos , Vírus da Influenza A Subtipo H1N1/genética , Vírus da Influenza A Subtipo H1N1/fisiologia , Influenza Humana/tratamento farmacológico , Influenza Humana/genética , Influenza Humana/virologia , Pulmão/metabolismo , Pulmão/virologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/genética , NF-kappa B/metabolismo , Baço/metabolismo , Baço/virologia , Receptor 4 Toll-Like/genética , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
19.
Pak J Pharm Sci ; 31(5(Special)): 2215-2221, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30463815

RESUMO

In this study the wound status of skin flap repair patients were closely observed, there were sign of infection. The secretion were taken for bacterial culture and sensitivity analysis and given sensitive antibiotics to active treatment. Patients received intravenous antibiotics 30 minutes before surgery to prevent infection. If postoperative infection occurred, according to susceptibility test results, patients were given sensitive antibiotics. Drug sensitivity analysis showed that 85.71% of gram-negative bacteria were sensitive to cefoperazone/shubatan and imipenem and 72.72% of gram-negative bacteria were sensitive to cefoperazone/tazobactam, so these 3 antibiotics were the first choice for treatment. And gram-positive bacteria were sensitive to teicoplanin and vancomycin. Therefore, scientific and rational use of antibiotics has great significance to effectively prevent postoperative infection and reduce the production of drugresistant bacteria. At the same time, L-extension deltopectoral flap was used to reconstruct a full-thickness labiomental defect concurrent infection. All the surgeries were successful without any necrosis. There are many kinds of pathogens for skin flap infection, and their sensitivity to antibiotics is not the same. Therefore, it is suggested that combination therapy should be carried out at the early stage, so as to ensure a good antibacterial effect.


Assuntos
Antibacterianos/uso terapêutico , Infecções Bacterianas/tratamento farmacológico , Lábio/microbiologia , Lábio/cirurgia , Complicações Pós-Operatórias/tratamento farmacológico , Dermatopatias Bacterianas/tratamento farmacológico , Feminino , Humanos , Masculino , Período Pós-Operatório , Transplante de Pele/métodos
20.
Front Immunol ; 9: 2297, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30337931

RESUMO

Aflatoxin B1 (AFB1), which alters immune responses to mammals, is one of the most common mycotoxins in feeds and food. Swine influenza virus (SIV) is a major pathogen of both animals and humans. However, there have been few studies about the relationship between AFB1 exposure and SIV replication. Here, for the first time, we investigated the involvement of AFB1 in SIV replication in vitro and in vivo and explored the underlying mechanism using multiple cell lines and mouse models. In vitro studies demonstrated that low concentrations of AFB1 (0.01-0.25 µg/ml) markedly promoted SIV replication as revealed by increased viral titers and matrix protein (M) mRNA and nucleoprotein (NP) levels in MDCK cells, A549 cells and PAMs. In vivo studies showed that 10-40 µg/kg of AFB1 exacerbated SIV infection in mice as illustrated by significantly higher lung virus titers, viral M mRNA levels, NP levels, lung indexes and more severe lung damage. Further study showed that AFB1 upregulated TLR4, but not other TLRs, in SIV-infected PAMs. Moreover, AFB1 activated TLR4 signaling as demonstrated by the increases of phosphorylated NFκB p65 and TNF-α release in PAMs and mice. In contrast, TLR4 knockdown or the use of BAY 11-7082, a specific inhibitor of NFκB, blocked the AFB1-promoted SIV replication and inflammatory responses in PAMs. Furthermore, a TLR4-specific antagonist, TAK242, and TLR4 knockout both attenuated the AFB1-promoted SIV replication, inflammation and lung damage in mice. We therefore conclude that AFB1 exposure aggravates SIV replication, inflammation and lung damage by activating TLR4-NFκB signaling.


Assuntos
Aflatoxina B1/farmacologia , Vírus da Influenza A/efeitos dos fármacos , Vírus da Influenza A/fisiologia , Pulmão/metabolismo , Pulmão/virologia , Receptor 4 Toll-Like/metabolismo , Liberação de Vírus/efeitos dos fármacos , Replicação Viral/efeitos dos fármacos , Aflatoxina B1/efeitos adversos , Animais , Linhagem Celular , Modelos Animais de Doenças , Técnicas de Silenciamento de Genes , Pulmão/patologia , Masculino , Camundongos , Infecções por Orthomyxoviridae/metabolismo , Infecções por Orthomyxoviridae/patologia , Infecções por Orthomyxoviridae/virologia , Transdução de Sinais , Vírus da Imunodeficiência Símia/metabolismo , Receptor 4 Toll-Like/genética
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