Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 51
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
2.
Food Funct ; 13(1): 425-436, 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34913451

RESUMO

Our previous study confirmed the beneficial effects of chestnut polysaccharides (CPs) on the spermatogenesis process, but the exact mechanism is not clear. Several studies have demonstrated the importance of balanced gut microbiota in maintaining normal reproductive function. In this study, we investigated the biological functions of CPs from the perspective of gut microbiota function, expecting to find out the specific mechanism of CPs in restoring impaired spermatogenesis. Compared with the control group, the mice treated with busulfan showed a reduced number of germ cells, structural changes in the small intestine and composition alteration in the gut microbiota at several levels, including the phylum and genus. In contrast, the number of germ cells in seminiferous tubules was significantly increased, and the structure of the small intestine and the composition of the gut microbiota were altered in the busulfan-treated mice after the CPs treatment. The 16s rRNA analysis results showed that the Firmicutes was the predominant phylum in all groups followed by Proteobacteria, Bacteroidetes, Actinobacteria, Tenericutes, Cyanobacteria and unidentified bacteria. Interestingly, the subsequent functional analysis implied that the steroid hormone biosynthesis process is the major metabolic pathway in the CPs-mediated restoration process and the experimental results confirmed this speculation. In conclusion, this study confirmed that CPs can restore the impaired spermatogenesis process by adjusting the gut microbiota and intestinal structure, which will also provide technical support and a theoretical basis for the subsequent treatment of male infertility.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34698529

RESUMO

Background: Lung malignancy is a main source of disease passing all throughout the planet, whereas the transthyretin (TTR) is a specific biomarker for clinical diagnosis. However, its role in lung malignancy stays to be obscure. Materials and Methods: In the current examination, the authors made an endeavor to research impact of abnormal expression of TTR on nonsmall cell lung carcinoma (NSCLC) by overexpression or knockdown of TTR. To further explore the instruments' fundamental mechanism part of TTR in NSCLC, several signal pathways were searched and verified. To confirm the effect of TTR overexpression on tumors, in vivo experiments were conducted. Result: It was found that upregulated TTR clearly stifled cell proliferation, migration, invasion, and expanded apoptosis. Significant suppression of phosphor-extracellular signal-regulated kinase (ERK) was observed in TTR-treated NSCLC cells, implying that TTR was important for cellular progress by regulating mitogen-activated protein kinase/ERK signaling pathway. In in vivo experiment, overexpression of TTR promoted cell apoptosis and inhibited tumor growth. Conclusion: Overall, the results suggest that TTR has a potential antitumor effect in human NSCLC progression, which provides theoretical basis for the diagnosis and treatment of NSCLC. Above all, further understanding of TTR was useful for clinical care. Clinical Trial Registration Number: 2016-08.

4.
Int J Biol Sci ; 17(7): 1744-1756, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33994859

RESUMO

Human papillomavirus (HPV) infection and gene mutations were reputed as key factors in cervical carcinoma (CC) and head and neck squamous cell carcinoma (HNSCC). However, the associations of HPV status and gene mutations remain to be determined. This study aims to identify molecular patterns of LRP1B mutation and HPV status via rewiring tumor samples of HNSCC (n=1478) and CC (n=178) from the TCGA dataset. Here, we found that LRP1B mutation was associated with HPV status in CC (P=0.040) and HNSCC (P=0.044), especially in HPV 16 integrated CC (P=0.036). Cancer survival analysis demonstrated that samples with LRP1B mutation showed poor disease outcomes in CC (P=0.013) and HNSCC (P=0.0124). In addition, the expression status of LPR1B was more favorable for prediction than TP53 or RB1 in CC and HNSCC. Mutation clustering analysis showed that samples with LRP1B mutation showed higher mutation count in CC (P=1.76e-67) and HNSCC (P<10e-10). Further analysis identified 289 co-occurrence genes in these two cancer types, which were enriched in PI3K signaling, cell division process, and chromosome segregation process, et al. The 289-co-occurrence gene signature identified a cluster of patients with a higher portion of copy number variation (CNV) lost in the genome, different tumor HPV status (P<10e-10), higher mutation count (P<10e-10), higher fraction genome altered value (P=2.078e-4), higher aneuploidy score (P=3.362e-4), and earlier started the smoking year (P=2.572e-4), which were associated with shorter overall survival (P=0.0103) in CC and HNSCC samples. Overall, LRP1B mutation was associated with tumor HPV status and was an unfavorable prognostic biomarker for CC and HNSCC.

5.
Front Cell Dev Biol ; 8: 585541, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33195232

RESUMO

Circular RNA (circRNA) has been increasingly proven as a new type of promising therapeutic RNA molecule in a variety of human diseases. However, the role of circRNA in bronchopulmonary dysplasia (BPD) has not yet been elucidated. Here, a new circRNA circABCC4 was identified from the Agilent circRNA chip as a differentially expressed circRNA in BPD. The relationship between circABCC4 level and BPD clinicopathological characteristics was analyzed. The function of circABCC4 was evaluated by performing CCK-8 and apoptosis analysis in vitro and BPD model analysis in vivo. RNA immunoprecipitation (RIP), luciferase reporter and rescue experiments were used to elucidate the interaction between circABCC4 and miR-663a. Luciferase reporter assay and rescue experiments were used to elucidate the interaction between PLA2G6 and miR-663a. CircABCC4 and PLA2G6 levels were increased, while miR-663a levels were decreased in the BPD group, compared to the control group. MiR-663a inhibited apoptosis by repressing PLA2G6 expression, while circABCC4 enhanced the apoptosis and inhibited the proliferation of A549 cells by sponging miR-663a and increasing PLA2G6 expression. In conclusion, circABCC4 promotes the evolving of BPD by spongening miR-663a and up-regulating PLA2G6 expression, which makes circABCC4 an ideal molecular target for early diagnosis and intervention of BPD.

6.
Aging (Albany NY) ; 12(12): 11431-11445, 2020 06 21.
Artigo em Inglês | MEDLINE | ID: mdl-32568099

RESUMO

Recently there has been a continuing worldwide decrease in the quality of human spermatozoa, especially in spermatozoa motility and concentration. Many factors are involved in this decline, and great efforts have been made to rescue spermatogenesis; however, there has been little progress in the improvement of sperm quality. Chestnuts are used in traditional Chinese medicine; their major active components are chestnut polysaccharides (CPs). CPs have many biological activities but their effects on spermatogenesis are unknown. The current investigation was designed to explore the impact of CPs on spermatogenesis and the underlying mechanisms. We demonstrated that CPs significantly increased sperm motility and concentration (4-fold and 12-fold, respectively), and improved seminiferous tubule development by increasing the number of germ cells after busulfan treatment. CPs dramatically rescued the expression of important genes and proteins (STRA8, DAZL, SYCP1, SYCP3, TNP1 etc.) in spermatogenesis. Furthermore, CPs increased the levels of hormone synthesis proteins such as CYP17A1 and HSD17ß1. All the data suggested that CPs improved the testicular microenvironment to rescue spermatogenesis. With CPs being natural products, they may be an attractive alternative for treating infertile patients in the future. At the same time, the deep underlying mechanisms of their action need to be explored.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Fagaceae/química , Infertilidade Masculina/tratamento farmacológico , Polissacarídeos/farmacologia , Espermatogênese/efeitos dos fármacos , Animais , Bussulfano/toxicidade , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/uso terapêutico , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Infertilidade Masculina/induzido quimicamente , Infertilidade Masculina/patologia , Masculino , Camundongos , Modelos Animais , Polissacarídeos/uso terapêutico , RNA-Seq , Túbulos Seminíferos/efeitos dos fármacos , Túbulos Seminíferos/crescimento & desenvolvimento , Túbulos Seminíferos/metabolismo , Túbulos Seminíferos/patologia , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Motilidade Espermática/genética , Espermatogênese/genética , Espermatozoides/efeitos dos fármacos , Espermatozoides/crescimento & desenvolvimento , Espermatozoides/metabolismo
7.
J Cancer ; 11(10): 2921-2934, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32226507

RESUMO

Background: Lung cancer is the most common cancer worldwide, both in terms of the incidence and mortality. NDC80 complex comprising of NDC80, NUF2, SPC24, and SPC25 is a heterotetrameric protein complex located in the outer layer of the kinetochore and plays a critical role in mitosis. This study focuses on the effects of NDC80 complex genes on clinical features and prognosis in lung adenocarcinoma (LUAD). Materials and methods: Expression of NDC80 complex in LUAD and related clinical information was extracted from the TCGA website. NDC80 complex gene functional analysis and correlation analysis was conducted by using DAVID, BiNGO, Gene MANIA, STRING and GSEA. Survival probability was predicted by nomogram. Statistical analysis was used to predict NDC80 complex gene expression on clinical features and prognosis in patients with LUAD. Results: Expression of NDC80, NUF2, SPC24 and SPC25 was significantly elevated in LUAD tumors compared with normal tissues (P < 0.05). These genes showed diagnostic values for LUAD (P < 0.001 for each; area under the curve (AUC), 0.958, 0.968, 0.951, and 0.932 respectively); combinatorial analysis of these genes was more advantageous than single analysis alone (P < 0.001; AUC > 0.900 for each). Expression of both NDC80 and SPC25 correlated with the prognosis of LUAD (P < 0.001; AUC > 0.600 for each). Higher expression of NDC80, NUF2, SPC24 and SPC25 was associated with low overall survival (OS) in univariate analysis. Higher expression of NDC80 and SPC25 was associated with low OS in multivariate analysis. High expression of NDC80 combined with high expression of SPC25 was predictive of poor OS in LUAD in joint analysis. Conclusion: NDC80 complex gene might be an early indicator of diagnosis and prognosis of LUAD. The combined detection of NDC80, NUF2, SPC24 and SPC25 may become a new research direction in LUAD diagnosis and a new target for tumor targeted gene therapy.

8.
Mol Med Rep ; 21(1): 429-437, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31746387

RESUMO

The present study examined whether lipoxin A4 (LXA4) increases the expression of HO­1, and inhibits the production of interleukin 6 (IL­6) and monocyte chemotactic protein 1 (MCP­1) in LXA4­induced protection during hyperoxia­induced injury in murine lung epithelial cells (MLE­12) and what signal pathway may participate in the actions of LXA4 inhibiting IL­6 and MCP­1. MLE­12 cells were exposed to air or hyperoxia with or without pretreatment with LXA4, Zinc protoporphyrin IX (ZnPP­IX), IL­6, anti­IL­6, MCP­1, anti­MCP­1, inhibitors of p38 mitogen­activated protein kinase (p38 MAPK), protein kinase B (Akt) and extracellular signal­regulated kinase 1/2 (ERK1/2) signaling pathways. The cell survival rates, cell viability, apoptosis rates, expression of superoxide dismutase (SOD), heme oxygenase­1 (HO­1), IL­6 and MCP­1, and the activations of p38 MAPK, ERK1/2 and Akt were measured. LXA4 significantly increased the cell survival rates, cell viability, SOD levels and HO­1 expression, reduced the apoptosis rates, and inhibited the MCP­1 and IL­6 levels induced by hyperoxia in cells. ZnPP­IX, an inhibitor of HO­1, blocked LXA4­induced protection on cell viability in cells exposed to hyperoxia. Anti­IL­6 and anti­MCP­1 improved the cell viability of cells exposed to hyperoxia. Inhibition of p38 MAPK and ERK1/2 blocked the expression of MCP­1 and IL­6 induced by hyperoxia. LXA4 inhibited the activation of p38 MAPK and ERK1/2 induced by hyperoxia, and increased the activation of the Akt signaling pathway, which was inhibited by hyperoxia. Therefore, LXA4 attenuated hyperoxia­induced injury in MLE­12 cells via the upregulation of HO­1 expression. The protection of LXA4 in hyperoxia­induced cell injury may be associated with the downregulation IL­6 and MCP­1 levels via the inhibition of the p38 MAPK and ERK1/2 signaling pathways.


Assuntos
Quimiocina CCL2/genética , Heme Oxigenase-1/genética , Lipoxinas/genética , Lesão Pulmonar/genética , Animais , Proliferação de Células/genética , Sobrevivência Celular/genética , Citocinas/genética , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Regulação da Expressão Gênica/genética , Humanos , Interleucina-6/genética , Lesão Pulmonar/patologia , Sistema de Sinalização das MAP Quinases/genética , Camundongos , Proteínas Proto-Oncogênicas c-akt/genética
9.
Curr Pharm Biotechnol ; 21(1): 79-88, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31580250

RESUMO

BACKGROUND: Large-scale population studies showed that the SNP rs1764391 of Connexin37 gene also known as Cx37 gene may play a pivotal role in the occurrence and development of acute myocardial infarction (AMI). Published results, however, are highly controversial. OBJECTIVE: This study aimed to examine the association between SNP rs1764391 of Cx37 and diseasesusceptibility, several risk factors, and gene-environment interactions of AMI in Guangxi Han Chinese population. METHODS: In this study, 344 healthy controls and 344 AMI patients of Han Chinese population were enrolled. The TaqMan assay was implemented to identify genotypes of Cx37 and allele frequencies of SNP rs1764391 in both the AMI and control groups. RESULTS: Significant differences were detected in TT genotype frequencies of SNP rs1764391 between the AMI and control groups (P < 0.05). In the context of gender stratification, the result was also statistically different in women (P < 0.05). Each variable such as age, BMI, diabetes, high blood pressure, smoking and TC was a risk factor and correlated significantly (P < 0.05) with the development of AMI. HDL-C correlated negatively with the risk of AMI (P < 0.001). BMI, smoking or alcohol consumed interacts significantly (P < 0.017) with the presence of the SNP rs1764391 CC genotype. CONCLUSION: Evidences were presented that Cx37 rs1764391 variation may contribute to the risk for AMI, especially in women and this genetic variant may prove to be a potential biomarker for AMI risk stratification and may prove to be a useful target for therapeutic intervention to further improve prognosis in high-risk patients.


Assuntos
Conexinas/genética , Infarto do Miocárdio/genética , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , China , Feminino , Frequência do Gene , Interação Gene-Ambiente , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Fatores de Risco
10.
Brain Topogr ; 32(6): 1035-1048, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31583493

RESUMO

Cortical folding is a hallmark of brain topography whose variability across individuals remains a puzzle. In this paper, we call for an effort to improve our understanding of the pli de passage phenomenon, namely annectant gyri buried in the depth of the main sulci. We suggest that plis de passage could become an interesting benchmark for models of the cortical folding process. As an illustration, we speculate on the link between modern biological models of cortical folding and the development of the Pli de Passage Frontal Moyen (PPFM) in the middle of the central sulcus. For this purpose, we have detected nine interrupted central sulci in the Human Connectome Project dataset, which are used to explore the organization of the hand sensorimotor areas in this rare configuration of the PPFM.


Assuntos
Córtex Cerebral/anatomia & histologia , Lobo Occipital/anatomia & histologia , Córtex Cerebral/fisiologia , Conectoma , Mãos , Humanos , Imageamento por Ressonância Magnética , Masculino , Modelos Biológicos , Lobo Occipital/fisiologia , Córtex Sensório-Motor/anatomia & histologia , Córtex Sensório-Motor/fisiologia
11.
Int J Mol Med ; 44(4): 1399-1413, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31432143

RESUMO

At present, thousands of circular RNAs (circRNAs) have been found in cancer and various tissues from different species. However, the expression of circRNAs during rat lung development remains largely unknown. In the present study, circRNA expression profiles were screened in three mixed rat lung tissues at 3 time­points [embryonic day (E) 19, E21 and post­natal (P) day 3] during fetal rat development with circRNA high­throughput sequencing. Preliminary results were verified by reverse transcription­PCR (RT­PCR) at 4 time­points (E16, E19, E21 and P3). A total of 375 circRNAs were differently expressed in E19 vs. E21 (fold change ≥1.5; P<0.05). At the same time, a total of 358 circRNAs were differently expressed in E21 vs. P3 (fold change ≥1.5; P<0.05). A total of 3 circRNAs (rno_circ:chr7:24777879­24784993, rno_circ:chr14:14620910­14624933 and rno_circ:chr3:1988750­â€‹1998592) were characterized by having consistent fold changes (≥1.5) between 3 time­points (E19, E21 and P3) and were selected for RT­PCR at 4 time­points (E16, E19, E21 and P3). Subsequently, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of parent genes of the differentially expressed circRNAs revealed that these circRNAs may serve important roles in lung development. The present results support that these new found circRNAs participate in lung development. Furthermore, these findings may help to clarify the physiopathological mechanism of normal rat lung development, and may further provide a physiopathological basis of lung developmental diseases.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Pulmão/embriologia , Pulmão/metabolismo , Organogênese/genética , RNA Circular/genética , Animais , Biologia Computacional/métodos , Feminino , Imunofluorescência , Perfilação da Expressão Gênica , Ontologia Genética , Redes Reguladoras de Genes , Pulmão/citologia , MicroRNAs/genética , Gravidez , Ratos
12.
Mol Med Rep ; 19(6): 4980-4988, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31059017

RESUMO

Deficiency of surfactant proteins (SPs) is the main cause of respiratory distress syndrome (RDS) and chronic lung diseases. Our previous study demonstrated that miR­431 was differentially expressed between infants with RDS and infants without RDS using microarray analysis. However, the potential role of miR­431 in the development of lung function is still unknown. In the present study, the morphological characteristics of lung tissues and the expression levels of miR­431 were examined at three time points of rat lung development [gestational days 19 and 21 (E19, and E21) and postnatal day (P3)]. The protein and mRNA levels of SMAD4 and SPs (SP­A, SP­B, SP­C and SP­D) were also validated by reverse transcription­quantitative polymerase chain reaction (RT­qPCR) and western blot analysis, respectively. The expression levels of miR­431 were gradually decreased over time periods of E19, E21 and P3, as determine using RT­qPCR and fluorescence in situ hybridization. Dual luciferase­reporter assays revealed that SMAD4 is a direct target of miR­431. The mRNA and protein expression levels of SMAD4 and SPs increased gradually in rat lung tissues from E19 to P3. The order of magnitude was as follows: E19, E21 and P3. The present study demonstrated that the expression level of miR­431 decreased in the order of E19, E21 and P3 during rat lung development. The target gene of miR­431, SMAD4, was negatively regulated by miR­431, and its expression levels in the rat lung tissue increased from E19 to the P3. Surfactant synthesis was further increased over the E19 to P3 time period. Further studies are required to determine how miR­431 regulates pulmonary surfactant synthesis by targeting SMAD4.


Assuntos
Pulmão/crescimento & desenvolvimento , MicroRNAs/metabolismo , Regiões 3' não Traduzidas , Animais , Animais Recém-Nascidos , Sequência de Bases , Feminino , Pulmão/metabolismo , Pulmão/patologia , MicroRNAs/genética , Microscopia Eletrônica , Gravidez , Proteínas Associadas a Surfactantes Pulmonares/genética , Proteínas Associadas a Surfactantes Pulmonares/metabolismo , Ratos , Ratos Sprague-Dawley , Alinhamento de Sequência , Proteína Smad4/química , Proteína Smad4/genética , Proteína Smad4/metabolismo
13.
J Pineal Res ; 67(1): e12577, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30938853

RESUMO

This study, using an in vitro ovary culture model, investigates the mechanisms through which di(2-ethylhexyl)phthalate (DEHP) impairs germ cell cyst breakdown and primordial follicle assembly. The results indicate the latter effects exerted by 10 or 100 µmol/L DEHP in cultured newborn ovaries were associated with increased levels of reactive oxygen species (ROS) and apoptosis. Based on a transcriptome analysis, we found the expression of the oxidative stress-related gene Xdh (xanthine dehydrogenase) was significantly upregulated in DEHP-cultured ovaries. Two treatments, namely Xdh RNAi or the addition of melatonin to the ovary culture, inhibited the increase in Xdh expression and ROS levels caused by DEHP and, at the same time, reduced apoptosis and the impairment of primordial follicle assembly in the treated ovaries. Together, the results identify Xdh gene as one of the major targets of DEHP in newborn ovaries and that the consequent increased level of ROS is possibly responsible for the increment of apoptosis and primordial follicle assembly impairment. At the same time, they highlight that melatonin alleviates the effects of DEHP as with other endocrine-disrupting compounds on the ovary.


Assuntos
Dietilexilftalato/toxicidade , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Melatonina/farmacologia , Ovário/enzimologia , Regulação para Cima/efeitos dos fármacos , Xantina Desidrogenase/biossíntese , Animais , Animais Recém-Nascidos , Apoptose/efeitos dos fármacos , Feminino , Camundongos , Ovário/patologia , Espécies Reativas de Oxigênio/metabolismo
14.
J Neurol Sci ; 399: 199-206, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30849580

RESUMO

BACKGROUND: Antiplatelet therapies for secondary prevention of ischemic stroke or transient ischemic attack (TIA) is a highly active research topic with five critical drugs obtained by visual analysis. We aimed to compare and rank multiple antiplatelet therapies using a network meta-analysis. METHODS: Relevant medical databases were searched. Eligible randomized controlled trials (RCTs) which examined any comparisons involving mono- or dual antiplatelet therapies, based on aspirin, clopidogrel, dipyridamole, ticlopidine, cilostazol and placebo for patients with noncardioembolic ischemic stroke or TIA, were included. 14 outcomes were assessed. Primary outcomes were stroke recurrence, composite events (stroke recurrence, myocardial infarction and vascular death), and intracranial hemorrhage. PROSPERO registered number CRD42017069728. RESULTS: 45 RCTs with 173,131 patients were included in network meta-analysis, involving eight antiplatelet therapies. Cilostazol and clopidogrel were statistically more efficacious than aspirin (odds ratio (OR) = 0.64, 95% confidence interval (CI) = 0.47-0.88; OR = 0.77, 95%CI = 0.62-0.95) and dipyridamole (OR = 0.64, 95%CI = 0.44-0.93; OR = 0.76, 95%CI = 0.58-0.99) in reducing stroke recurrence, and showed significant benefits in reducing composite events compared with aspirin (OR = 0.63, 95%CI = 0.45-0.89; OR = 0.90, 95%CI = 0.83-0.97). No significant difference was found between cilostazol and clopidogrel in intracranial hemorrhage. Weighted regression suggested cilostazol was hierarchically the optimum treatment in consideration of both efficacy and safety, followed by clopidogrel. CONCLUSION: Cilostazol and clopidogrel are probably promising options for secondary prevention of ischemic stroke or TIA. Both of them reduce stroke recurrence similarly compared with aspirin or dipyridamole, and reduce composite events compared with aspirin. Further studies are needed to confirm this finding.


Assuntos
Isquemia Encefálica/prevenção & controle , Ataque Isquêmico Transitório/prevenção & controle , Inibidores da Agregação Plaquetária/uso terapêutico , Acidente Vascular Cerebral/prevenção & controle , Isquemia Encefálica/tratamento farmacológico , Humanos , Ataque Isquêmico Transitório/tratamento farmacológico , Metanálise em Rede , Prevenção Secundária , Acidente Vascular Cerebral/tratamento farmacológico
15.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(3): 287-293, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-30907356

RESUMO

OBJECTIVE: To study the role of miR-431 in lung development and morphology. METHODS: According to the stage of lung development in rats, Sprague-Dawley rats at embryonic day 16 (E16), embryonic day (E19), embryonic day (E21), postnatal day 1 (P1), postnatal day 3 (P3), postnatal day 7 (P7), postnatal day 14 (P14) and 10 weeks after birth (P10 weeks) were selected, and lung tissue samples were collected for observation. Hematoxylin-eosin staining and transmission electron microscopy were performed to observe the morphology of lung tissue. Fluorescence in situ hybridization and real-time PCR were used to measure the expression of miR-431 during the critical stages of lung development (E19, E21 and P3). RESULTS: The E19 group had the formation of the lamellar body and type II alveolar epithelial cells in the fetal lung tissue. The number of lamellar bodies increased with the increasing gestational age, with aggregation and excretion. Pulmonary alveoli formed rapidly, the lung interstitium became thinner, and the microvascular system became mature after birth. Fluorescence in situ hybridization and real-time PCR showed that the expression of miR-431 gradually decreased with the increasing gestational age (P<0.05). CONCLUSIONS: The systematic and continuous morphological data of lung development is obtained in this experiment. In addition, miR-431 may play an important role in the negative regulation of lung development, which provides basis and direction for further research on the mechanism of lung development and related diseases.


Assuntos
Pulmão , Animais , Feto , Hibridização in Situ Fluorescente , MicroRNAs , Ratos , Ratos Sprague-Dawley
16.
J Cell Physiol ; 234(8): 14339-14350, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30633354

RESUMO

From the previous research, it has been supported that activin A (ActA) is conducive to ovarian development in vitro. In the present paper, with the aim to identify the molecular pathways through which ActA can influence processes of the fetal and early postnatal oogenesis, we analyzed the transcriptome of embryonic ovaries (12.5 days postcoitum) in vitro cultured with or without ActA for 6 days, as well as the produced oocytes for 28 days, and further compared the gene expression profile with their in vivo counterparts. With the confirmation of designed test, we found that the addition of ActA to the ovary culture tended, generally, to align oocyte gene expression to the in vivo condition, in particular of a number of genes involved in meiosis and epigenetic modifications of histones. In particular, we identified DNA recombination during the oocyte meiotic prophase I and lysine trimethylation of the histone H3K27 during the oocyte growth phase as molecular pathways modulated by ActA.


Assuntos
Ativinas/genética , Meiose/genética , Oogênese/genética , Transcriptoma/genética , Animais , Apoptose/genética , Feto , Código das Histonas/genética , Histona Desmetilases com o Domínio Jumonji/genética , Camundongos , Oócitos/crescimento & desenvolvimento , Oócitos/metabolismo
17.
Zhonghua Nan Ke Xue ; 25(8): 713-718, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32227714

RESUMO

Objective: To investigate the clinical effect of suprapubic V-Y incision versus that of Sun's coronal sulcus ring incision plus suprapubic liposuction (CSRI+SPLS) for penile elongation. METHODS: From December 2010 to January 2018, 100 adult males with congenital short penis underwent suprapubic V-Y incision (the V-Y group, n = 50) or CSRI+SPLS (n = 50) for penile elongation surgery in our department. We statistically analyzed the clinical data on the two groups of patients, including age, body mass index (BMI), pre- and post-operative penile lengths in flaccid and erectile states, operation time, intraoperative blood loss, postoperative use of analgesics, postoperative hospital stay, stage-Ⅰ wound healing, incidence of postoperative complications, and quality of pre- and post-operative sexual life. RESULTS: The average ages of the patients in the V-Y and CSRI+SPLS groups were 23.5 and 23.0 years, their BMIs (26.59 ± 1.16) and (26.44 ± 0.96) kg/m2, preoperative flaccid penile lengths (5.11 ± 0.30) and (5.12 ± 0.35) cm, preoperative erectile penile lengths (7.57 ± 0.65) and (7.35 ± 0.59) cm, postoperative flaccid penile lengths (7.80 ± 0.40) and (7.79 ± 0.42) cm, postoperative erectile penile lengths (11.59 ± 0.55) and (11.47 ± 0.64) cm, none with statistically significant difference between the two groups (P > 0.05). Compared with the V-Y group, the CSRI+SPLS group showed a markedly shorter operation time (108 ï¼»90-120ï¼½ vs 51 ï¼»45-58ï¼½ min, P < 0.01), less intraoperative blood loss (30 ï¼»15-45ï¼½ vs 15 ï¼»5-25ï¼½ ml, P < 0.01), shorter postoperative hospital stay 8 ï¼»6-11ï¼½ vs 4 ï¼»2-6ï¼½ d, P < 0.01), lower incidence rates of postoperative hematoma (23.33% ï¼»7 casesï¼½ vs 0, P < 0.05) and infection (20.00% ï¼»6 casesï¼½ vs 0, P < 0.05), higher stage-Ⅰ healing rate (76.67% ï¼»23 casesï¼½ vs 100.00% ï¼»30 casesï¼½, P < 0.05), lower rate of postoperative use of analgesics (26.67% ï¼»8 casesï¼½ vs 3.33% ï¼»1 caseï¼½, P < 0.05), and higher score on the quality of postoperative sexual life (36.73 ± 5.41 vs 42.07 ± 3.64, P < 0.01) though with no statistically significant difference preoperatively (28.70 ± 4.87 vs 28.27 ± 3.40, P > 0.05). CONCLUSIONS: As a surgical procedure for penile elongation, Sun's coronal sulcus ring incision plus suprapubic liposuction is superior to suprapubic V-Y incision for its advantages of lower invasiveness, shorter operation time, less intraoperative blood loss, and lower incidence of postoperative complications.


Assuntos
Lipectomia , Pênis/cirurgia , Procedimentos Cirúrgicos Urológicos Masculinos/métodos , Perda Sanguínea Cirúrgica , Humanos , Masculino , Duração da Cirurgia , Complicações Pós-Operatórias , Adulto Jovem
18.
Zhonghua Nan Ke Xue ; 25(10): 883-889, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-32233218

RESUMO

Objective: To study the safety, durability and effectiveness of a new medical liquid silicone rubber elastomer (LSRE) compound Urolastic in penile augmentation in beagle dogs. METHODS: A total of 30 beagle dogs were included in the experiment. The diameters of the proximal, middle and distal penis of the animals were measured and the indexes of blood routine, liver and kidney function and electrolytes obtained before and at 1 week and 3 months after penile subcutaneous injection of the LSRE compound. CT scanning and pathological examinations of the liver, kidney and penile tissues were performed at 1 and 3 months after treatment. RESULTS: The diameters of the proximal, middle and distal penis of the dogs were increased by (0.4 ± 0.3) cm, (0.6 ± 0.1) cm and (0.5 ± 0.3) cm at 1 week, and (0.4 ± 0.2) cm, (0.5 ± 0.1) cm and (0.6 ± 0.2) cm at 3 months after injection of the LSRE compound, with statistically significant difference from the baseline (P < 0.01 or P < 0.05) but not between the 1st week and the 3rd month (P > 0.05). The counts of leukocytes and neutrophils were markedly increased compared with the baseline (ï¼»18.16 ± 2.57ï¼½ vs ï¼»15.16 ± 3.17ï¼½ g/L, P < 0.05; ï¼»77.34 ± 9.21ï¼½% vs ï¼»67.18 ± 8.25ï¼½%, P < 0.05), but not the other blood routine indexes. There were no statistically significant differences in the liver and kidney functions or electrolytes before and after the injection. At 1 and 3 months after treatment, Urolastic was clearly visible and the injection points were irregular in shape at CT imaging. The anatomical findings were consistent with the CT manifestations and showed that the material was easily separated from the surrounding tissues. No significant inflammatory cell infiltration was observed in pathological examinations at 1 and 3 months. CONCLUSIONS: The new medical LSRE compound Urolastic has a good clinical application prospect in penile augmentation for its advantages of significant effectiveness and high safety.


Assuntos
Dimetilpolisiloxanos/administração & dosagem , Pênis/anatomia & histologia , Borracha , Elastômeros de Silicone , Titânio/administração & dosagem , Animais , Cães , Injeções , Masculino , Pênis/efeitos dos fármacos
19.
J Cell Biochem ; 120(3): 4238-4247, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30269353

RESUMO

OBJECTIVE: This study aimed to explore the effects of high-mobility group B1 (HMGB1) on coronary microembolization (CME)-induced myocardial inflammation, myocardial apoptosis, and cardiac function injury in rats. METHODS: Forty Sprague-Dawley rats were divided into sham operation group (sham group), microembolization group (CME group), CME + HMGB1 siRNA (HMGB1 siRNA) group, and CME + scrambled siRNA (control siRNA) group (10 rats in each group). The CME model group was constructed by injecting microembolism spheres into the apex of the left ventricle after clamping the ascending aorta. The sham group was constructed by injecting the same amount of saline. The HMGB1 siRNA group was injected with HMGB1 siRNA transfection complex via the tail vein 72 hours before CME modeling. The control siRNA group was injected with the same amount of scrambled siRNA mixture through the tail vein 72 hours before CME modeling. The cardiac function, serum cardiac troponin I level, and apoptotic index were examined 12 hours after the surgery. The levels of HMGB1, nuclear factor-κB (NF-κB) p65, glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), cleaved caspase-12, cleaved caspase-3, tumor necrosis factor-α (TNF-α), and interleukin 1ß (IL-1ß) were detected. RESULTS: Myocardial dysfunction, enhanced serum cardiac troponin I level, and apoptotic index were induced following CME. Moreover, CME increased the expression of HMGB1, NF-κB p65, GRP78, CHOP, cleaved caspase-12, cleaved caspase-3, TNF-α, and IL-1ß. HMGB1 siRNA reversed these effects, whereas scrambled siRNA had no effect. CONCLUSIONS: Inhibition of HMGB1 expression reduced CME-induced myocardial injury and improved cardiac function. Hence, it may serve as a new target for preventing and treating the CME-induced myocardial injury.


Assuntos
Vasos Coronários/patologia , Embolia/complicações , Proteína HMGB1/genética , Proteína HMGB1/metabolismo , Miocardite/etiologia , Miocardite/metabolismo , Animais , Apoptose/genética , Caspase 12/metabolismo , Caspase 3/metabolismo , Proteínas de Choque Térmico/metabolismo , Interleucina-1beta/metabolismo , Masculino , Miócitos Cardíacos/metabolismo , RNA Interferente Pequeno/genética , Ratos , Ratos Sprague-Dawley , Volume Sistólico/genética , Fator de Transcrição CHOP/metabolismo , Fator de Transcrição RelA/metabolismo , Transfecção , Troponina I/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
20.
Cancer Biother Radiopharm ; 34(2): 76-84, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30585765

RESUMO

OBJECTIVE: To predict the early identification of recurrence based on magnetic resonance imaging (MRI) in nasopharyngeal cancer (NPC) patients. METHODS: The clinical and MRI data of 215 patients with local recurrent NPC were retrospectively reviewed. Logistic regression analysis was performed to distinguish the independent risk factors for the short-term (less than 24 months) local recurrence of NPC. The predictive score model was based on the regression coefficients of significant independent variables. RESULTS: Residual disease in the nasopharyngeal cavity (NC), masticator space invasion (MSI), skull base bone erosion (SBBE), and MRI-detected cranial nerve invasion (MDCNI) were all significant independent risk factors for the short-term recurrence of NPC (p < 0.05). The receiver operating characteristic curve showed that the total score had a maximal AUC (area under the curve) value of 0.897, with a cutoff point of 10.50. The sensitivity and specificity were 79.4% and 80.5%, respectively. CONCLUSION: Residual lesions in NC, MSI, SBBE, and MDCNI are independent risk factors in predicting the short-term recurrence of NPC. The authors' findings suggest that patients with a score of more than 10.50 points should be hypervigilant regarding the possibility of short-term recurrence.


Assuntos
Imageamento por Ressonância Magnética/métodos , Carcinoma Nasofaríngeo/diagnóstico por imagem , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo/patologia , Recidiva Local de Neoplasia , Estudos Retrospectivos , Sensibilidade e Especificidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...