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1.
Food Chem ; 304: 125383, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31479997

RESUMO

An aqueous two-phase system was used in conjunction with ultrasonic cell disruption to extract and separate solanine (mainly solasonine and solamargine) and Solanum nigrum polysaccharide from Solanum nigrum unripe fruit. The optimized conditions of the present study were determined by a single-factor experiment and a multifactor experiment. The concentration of ethanol was set at 60% and the duration of the ultrasonic cell disruption extraction was 50 min. In the ethanol-K2CO3 aqueous two-phase separation system, the concentration of ethanol was 36%, the concentration of K2CO3 was 0.21 mg·mL-1, and the temperature was 15 °C. The solasonine and solamargine were determined by high-performance liquid chromatography, and the Solanum nigrum polysaccharide was determined by an ultraviolet-visible spectrophotometer in accordance with the phenol-sulfuric acid method. xUnder optimized conditions, the average extraction efficiencies of solasonine, solamargine and Solanum nigrum polysaccharide were 95.86%, 95.95% and 96.95%, respectively, and the average separation efficiencies of solasonine, solamargine and Solanum nigrum polysaccharide were 2.07 mg·g-1, 2.05 mg·g-1 and 8.15 mg·g-1, respectively.

2.
Mol Med Rep ; 20(5): 4235-4243, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31545428

RESUMO

Proper spindle formation and accurate chromosome segregation are essential for ensuring mitotic fidelity. Phosphatase and tensin homolog (PTEN) is a multifunctional protein, which is able to maintain the stability of the genome and chromosomes. The present study described an essential role of PTEN in regulating chromosome segregation to prevent gross genomic instability via regulation of mitotic arrest deficient 2 (MAD2). PTEN knockdown induced cell cycle arrest and abnormal chromosome segregation, which manifested as the formation of anaphase bridges, lagging chromosomes and premature chromatid separation. In addition, MAD2 was identified as a potential target of PTEN. Furthermore, the present study revealed that PTEN knockdown resulted in MAD2 degradation via the ubiquitin­proteasomal pathway, while restoration of MAD2 expression partially ameliorated the mitotic defects induced by PTEN loss. The results from the present study proposed a novel mechanism by which PTEN maintains chromosome stability.

3.
Math Biosci Eng ; 16(5): 4607-4621, 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31499680

RESUMO

OBJECTIVE: To explore the potential mechanism which miR-527 targeting the heparan sulfate 6-O-endosulfatase (SULF2) regulates TGF-ß/SMAD signaling pathway induced epithelial-mesenchymal transition (EMT) in non-small-cell lung cancer (NSCLC). METHODS: 38 pairs of lung tumor biopsies and corresponding paracancerous biopsies were obtained from NSCLC patients with surgical resection, normal human bronchial epithelial BEAS-2B cells and five NSCLS cell lines were applied for our study. miR-527 and SULF2 expression were determined by qRT-PCR and immunohistochemistry. MiR-527 and SULF2 biological link were predicted by Targetscan.org and tested by dual luciferase. Cells proliferation and apoptosis were respectively detected by EDU staining and flow cytometry. Cells migration was examined by transwell and scratch-wound assay. Expression of proteins related to EMT and TGF-ß/SMAD signaling pathway, such as E-cadherin, N-cadherin, p-Samd3 and p-Smad2, was detected by western blot. RESULTS: miR-527 expression was decreased in lung tumor tissues and NSCLS cell lines, conversely, SULF2 expression was significantly increased. In addition, we found that miR-527 targeted 3'-untranslated regions (3'-UTR) of SULF2 and mediated its expression. Overexpression of miR-527 evidently suppressed NSCLC proliferation, invasion and EMT via TGF-ß/SMAD signaling pathway. Moreover, the silence of SULF2 exhibited a similar effect. CONCLUSION: miR-527 targeting SULF2 down-regulated SULF2 expression, concurrently, suppressed NSCLC epithelial-mesenchymal transition and invasion via inhibiting TGF-ß/SMAD signaling pathway.

4.
Math Biosci Eng ; 16(5): 5652-5671, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31499730

RESUMO

Deflection is a crucial indicator to reflect the operating condition of girder bridges, which can be used to evaluate structure condition and identify abnormal loading. The paper analyzed the deflection characteristics of long-span girder bridges based on the coupling vibration between stochastic traffic stream and bridge. First, the latest research advances were integrated to form an analytical model of the coupling vibration between stochastic traffic stream and bridge. Then, a generalized Pareto distribution model based on peaks-over-threshold theory was established to predict the extreme girder deflection. Next, a cellular automaton based microsimulation method was proposed to model the traffic loads on bridges, which utilized the intelligent driver car-following model and acceptance distance based lane-changing model. Finally, these theories were applied in the case study of a long-span prestressed concrete continuous girder bridge. It is discovered from the study that, under the coupling vibration between stochastic traffic stream and bridge, the predicted extreme deflection of the case bridge is far lower than the specified design value. Hence, a grading warning model was established and employed to the analysis of deflection monitoring data of the bridge, showing a wide potential prospect of application.

5.
Toxicol Lett ; 315: 1-8, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31421153

RESUMO

Arsenic trioxide (As2O3) has been used clinically for the treatment of acute promyelocytic leukemia and some solid tumors. However, the mechanisms of its anti-tumor effects are still elusive. Angiogenesis is a key process for tumor initiation, and increasing evidence has supported the role of anti-angiogenesis caused by arsenic in tumor suppression, although the detailed mechanism is not well understood. In the present study, we found that As2O3 significantly inhibited the angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro, and this was mediated by the upregulation of FoxO3a. Knockdown of FoxO3a could restore the angiogenic ability of HUVECs. Moreover, vascular endothelial cell-specific knockout of FoxO3a in mice could disrupt the anti-angiogenesis effect of As2O3 and endow the tumors with resistance to As2O3 treatments. Our results revealed a new mechanism by which As2O3 suppresses angiogenesis and tumor growth.


Assuntos
Inibidores da Angiogênese/uso terapêutico , Antineoplásicos/uso terapêutico , Trióxido de Arsênio/farmacologia , Trióxido de Arsênio/uso terapêutico , Proteína Forkhead Box O3/efeitos dos fármacos , Leucemia Promielocítica Aguda/tratamento farmacológico , Regulação para Cima/efeitos dos fármacos , Animais , Antineoplásicos/farmacologia , Crescimento Celular/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Modelos Animais de Doenças , Células Epiteliais/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos , Veias Umbilicais/efeitos dos fármacos
6.
Photochem Photobiol ; 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31441061

RESUMO

UVA can penetrate dermis and cause functional damage of dermal fibroblasts leading photoaging. Ginseng is a widely used traditional Chinese medicine for skin aging. However, its effects on skin photoaging induced by UVA are not clear. In this study, we isolated ginseng proteins (GP), with molecular weights of 27 kDa and 13 kDa, and found that they alleviated the inhibitory effects of UVA on cell viability and increased percentage of NIH-3T3 fibroblasts in the S phase of cells cycle. GP also improved cell contraction ability, increased the expression and secretion of CoL-I, similar to MAPK phosphorylation inhibitors and reduced expression and secretion of MMP-1, MMP-2 and MMP-9 as well as the enzyme activities of MMP-2 and MMP-9. They reduced ROS content, DNA damage and 8-OHdG content, as well as the protein expression of p53, p21 and p16. The levels of p-ERK, p-p38 and p-JNK, p-c-Fos and p-c-Jun proteins were decreased by GP. Inactivated GP did not inhibit the cellular activity and expression and secretion of CoL-I irradiated by UVA. The results showed that GP can improve cell viability and contractile function by inhibiting DNA damage and collagen degradation to inhibit the photoaging effects of skin dermal cells caused by UVA.

7.
Sensors (Basel) ; 19(9)2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31075934

RESUMO

Real-time and accurate monitoring of dynamic deflection is of great significance for health monitoring and condition assessment of bridge structures. This paper proposes an improved step-type liquid level sensing system (LLSS) for dynamic deflection monitoring. Layout of straight-line-type pipeline is replaced by step-type pipeline in this improved deflection monitoring system, which can remove the interference of the inclination angle on the measurement accuracy and is applicable for dynamic deflection monitoring. Fluid dynamics are first analyzed to demonstrate that measurement accuracy is interfered with by the fluid velocity induced by structural vibration, and ANSYS-FLOTRAN is applied for analyzing the influence range caused by the turbulent flow. Finally, a step-type LLSS model is designed and experimented with to verify the influence of the three key parameters (initial displacement excitation, step height, and distance from the measurement point to the elbow) on the measurement accuracy, and the reasonable placement scheme for the measurement point is determined. The results show that the measurement accuracy mainly depends on the turbulent flow caused by step height. The measurement error gets smaller after about 1.0 m distance from the elbow. To ensure that the measurement error is less than 6%, the distance between the measurement point and the elbow should be larger than 1.0 m.


Assuntos
Técnicas Biossensoriais/métodos , Articulação do Cotovelo/fisiologia , Humanos , Monitorização Fisiológica/métodos , Vibração
8.
Biomed Res Int ; 2019: 2737968, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31111045

RESUMO

Purpose: To investigate the effect of preoperative treatment and postoperative enhanced anti-inflammatory treatment on alleviating meibomian gland dysfunction (MGD) and dry eye induced by cataract surgery. Design: Prospective, randomized clinical trial. Methods: A total of 120 cataract patients with moderate obstructive-MGD were enrolled and randomized with 60:30:30 number of patients in cohorts I, II, and III, respectively: Cohort I: routine postoperative anti-inflammatory treatment; Cohort II: preoperative treatment (warming compress, lid hygiene, and anti-inflammatory treatment) and routine postoperative anti-inflammatory treatment; Cohort III: enhanced postoperative anti-inflammatory treatment. Main Outcomes Measures: All participants were examined preoperatively and postoperatively for ocular symptom score (OSS), noninvasive keratographic tear break-up time (NIKBUT), corneal fluorescein staining, Schirmer I test, lid margin, meibum quality and expressibility, and meibomian gland dropout. Results: Ocular surface disorders and MGD showed aggravated status at 1 month postoperatively in Cohort I and Cohort III, and the aggravated MGD resolved by 3 months postoperatively. At 1 month postoperatively, Cohort II and Cohort III presented high NIKBUT and low OSS, lid margin, and meibum quality and expressibility (Cohort II vs Cohort I: all P<0.001, respectively; Cohort III vs Cohort I: P=0.011, P=0.024, P=0.046, P=0.045, and P=0.012, respectively). Additionally, Cohort II had better outcomes of lid margin and meibum quality and expressibility than Cohort III at 1 month postoperatively (P=0.031, P=0.026, and P<0.001, respectively). At 3 months postoperatively, Cohort II presented a significantly higher NIKBUT than Cohort I and Cohort III (P<0.001 and P=0.001, respectively). Conclusion: Preoperative management of MGD is effective and optimal in alleviating obstructive-MGD and dry eye induced by cataract surgery.

10.
J Colloid Interface Sci ; 544: 321-328, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30861437

RESUMO

In this study, we demonstrated the influence of adding nanofiltration membranes to a CDI cell, when desalinating mono- and divalent salt solutions both theoretically and experimentally, and proposed a new procedure for the separation of mono- and divalent ions. It is revealed that the distinction of the diffusion coefficient between mono- and divalent ions is the key to the effective separation. Combined with the appropriate time of adsorption (1.5 h), the concentration ratio of NaCl to MgSO4 can be increased by a factor of 3, from a mixture with equal amount of NaCl and MgSO4.

11.
Indian J Ophthalmol ; 67(4): 484-489, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30900579

RESUMO

Purpose: Our study was conducted to evaluate and compare the accuracy of the refractive prediction determined by the calculation formulas for different intraocular lens (IOL) powers for high myopia. Methods: This study reviewed 217 eyes from 135 patients who had received cataract aspiration treatment and IOL implantation. The refractive mean numerical error (MNE) and mean absolute error (MAE) of the IOL power calculation formulas (SRK/T, Haigis, Holladay, Hoffer Q, and Barrett Universal II) were examined and compared. The MNE and MAE at different axial lengths (AL) were compared, and the percentage of every refractive error absolute value for each formula was calculated at ±0.25D, ±0.50D, ±1.00D, and ±2.00D. Results: In all, 98 patients were recruited into this study and 98 eyes of them were analyzed. We found that Barrett Universal II formula had the lowest MNE and MAE, SRK/T and Haigis formulas arrived at similar MNE and MAE, and the MNE and MAE calculated by Holladay and Hoffer Q formula were the highest. Barrett Universal II formulas have the lowest MAE among different AL patients, whereas it reached the highest percentage of refractive error absolute value within 0.5D in this study. The MAE of each formula is positively correlated with AL. Conclusion: Barrett Universal II formula rendered the lowest predictive error compared with SRK/T, Haigis, Holladay, and Hoffer Q formulas. Thus, Barrett Universal II formula may be regarded as a more reliable formula for high myopia.


Assuntos
Biometria/métodos , Extração de Catarata , Lentes Intraoculares , Miopia/fisiopatologia , Óptica e Fotônica , Complicações Pós-Operatórias/fisiopatologia , Refração Ocular/fisiologia , Idoso , Comprimento Axial do Olho , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Miopia/diagnóstico , Miopia/etiologia , Complicações Pós-Operatórias/diagnóstico , Reprodutibilidade dos Testes , Estudos Retrospectivos , Acuidade Visual
12.
J Pharm Biomed Anal ; 170: 112-123, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30921645

RESUMO

Irinotecan hydrochloride liposome injection (IHLI) is a formulation of anticancer drug irinotecan hydrochloride (CPT-11) entrapped in the aqueous core of liposomes. To understand the pharmacokinetic property and evaluate the relationship between pharmacokinetics and pharmacodynamics/toxicity of IHLI, it is of prime importance to determine the concentrations of free CPT-11, total CPT-11 and its main metabolites (SN-38 and SN-38 G) in human plasma. In this paper, we developed and validated a sensitive and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to quantify the concentrations of these related substances in human plasma. Free CPT-11, SN-38 and SN-38 G in human plasma were simultaneously separated and extracted by 96-well solid phase extraction (SPE) plate, while total CPT-11 was extracted by protein precipitation (PP) method. The analytes were chromatographed on an Acquity UPLC BEH C18 column and then detected on a Xevo TQ-S tandem mass spectrometer in multiple reactions monitoring (MRM) mode using positive electrospray ionization (ESI). The UPLC-MS/MS method combined with SPE and PP techniques were fully validated in line with existing guidelines issued by regulatory agencies. In brief, all the analytes achieved a satisfactory selectivity and sensitivity in this method. The calibration curves were proved to be linear over the concentration range of 10-10000 ng/mL for total CPT-11, 0.5-1000 ng/mL for free CPT-11, 0.5-200 ng/mL for SN-38 and SN-38 G, respectively. For all the analytes, the intra- and inter-run precisions were less than 11.4% and the accuracies (in terms of RE%) were within -7.7-7.3% except for accuracies of LLOQs were within -15.8 to 7.2%. Besides, carry-over, extraction recovery, matrix effect, dilution integrity, stability and special matrices were also assessed. Finally, the method was successfully applied to a phase I clinical pharmacokinetic study of IHLI in Chinese subjects with advanced solid tumors.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Irinotecano/sangue , Plasma/química , Espectrometria de Massas em Tandem/métodos , Administração Intravenosa/métodos , Humanos , Injeções/métodos , Lipossomos/química , Reprodutibilidade dos Testes , Extração em Fase Sólida/métodos
13.
Nanotechnology ; 30(22): 225201, 2019 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-30731433

RESUMO

In this paper, hybrid transparent conductive films (TCFs) are designed by combining silver nanowires with the single-wall carbon nanotubes (SWCNTs) and the transparent heating films (THFs) based on the TCFs are evaluated for possible vehicle applications. By comparing the properties, including the transmittance, sheet resistance, microstructure and heating curves, we found that the SWCNTs/AgNWs are considerably suitable for making THFs. The after-treatment methods, such as physical method (hot roll pressing) and chemical method (nitric acid and Poly (diallydimethylammonium chloride) solution, (PDAC)) were researched in detail to optimize the sheet resistance and transparency to fit the THF requirements. A careful study of the different after-treatment methods revealed that hot roll pressing can quickly and efficiently improve the properties, while the nitric acid is more helpful than PDAC for the long-term stability. The results showed that a small amount of SWCNTs addition can promote the endurable maximum electric current by spreading the heat fast and efficiently, and the maximum current flow can be as high as 4 A. The thermal stability of the THFs and the de-frog performance were tested, indicating that the hybrid film had an advantage in resisting current shock and good thermal efficiency was obtained. The fabricated TCFs of stable thermal properties are qualified as a windshield-glass heater.

14.
Chin J Integr Med ; 2018 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-30519874

RESUMO

OBJECTIVE: To determine the effects of hawthorn extract on serum lipid levels, pathological changes in aortic atherosclerosis plaque, inflammatory factors, and apoptosis-related protein and mRNA expression in apolipoprotein E gene knockout (ApoE-/-) mice. METHODS: Thirty-six ApoE-/- mice were fed with a high-fat diet starting at the age of 8 weeks. Mice were randomly divided into 3 groups by a random number table including model group, hawthorn extract group, and simvastatin group, 12 mice in each group. Twelve 8-week-old C57BL/6 mice were fed a basic diet and served as control. The mice in the control and model groups were administered 0.2 mL saline daily, the mice in the hawthorn extract and simvastatin groups were administered with 50 mg/kg hawthorn extract or 5 mg/kg simvastatin daily for 16 weeks. After 16 weeks, plasma lipids including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were determined by an enzymatic assay. Aortic atherosclerotic lesions were observed by light microscopy, scanning and transmission electron microscopy, respectively. Plasma levels of monocyte chemoattractant protein-1 (MCP-1), interleukin-1ß (IL-1ß), adiponectin (APN), and hypersensitive C-reactive protein (hs-CRP) were measured by enzyme-linked immunosorbent assay (ELISA). Protein and mRNA expressions of Bax and Bcl-2 in the aorta were assessed by Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. RESULTS: Compared to the control group, the plasma levels of TC, TG and LDL-C were significantly increased and HDL-C were significantly decreased in the model group (P<0.01). Compared to the model group, treatment with hawthorn extract significantly decreased the plasma levels of TC, TG, and LDL-C and increased the plasma level of HDL-C in ApoE-/- mice (P<0.01). The levels of MCP-1, IL-1ß, and hs-CRP in the model group were significantly increased and APN was significantly decreased compared with the control group (P<0.01). Compared to the model group, treatment with hawthorn extract decreased the levels of MCP-1, IL-1ß, and hs-CRP and increased the APN level (P<0.01). Compared to the control group, the protein and mRNA expression of Bax in the model group were significantly increased and the expression of Bcl-2 was significantly decreased (P<0.01). Hawthorn extract also reduced the protein and mRNA expression of Bax and increased the Bcl-2 expression in the aorta (P<0.01). CONCLUSION: Hawthorn extract has anti-atherosclerosis and stabilizing unstable plaque effects. The mechanism may be related to the inflammation and apoptosis signaling pathways.

15.
Molecules ; 24(1)2018 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-30577538

RESUMO

Panax ginseng C.A. Meyer is one of the most important medicinal plants in Northeast China, and ginsenosides are the main active ingredients found in medicinal ginseng. The biosynthesis of ginsenosides is regulated by environmental factors and the expression of key enzyme genes. Therefore, in this experiment, ginseng in the leaf opened stage, the green fruit stage, the red fruit stage, and the root growth stage was used as the test material, and nine individual ginsenosides and total saponins (the sum of the individual saponins) were detected by HPLC (High Performance Liquid Chromatography). There was a trend of synergistic increase and decrease, and saponin accumulation and transfer in different tissues. The expression of key enzyme genes in nine synthetic pathways was detected by real-time PCR, and the correlation between saponin content, gene expression, and ecological factors was analyzed. Correlation analysis showed that in root tissue, PAR (Photosynthetically Active Radiation) and soil water potential had a greater impact on ginsenoside accumulation, while in leaf tissue, temperature and relative humidity had a greater impact on ginsenoside accumulation. The results provide a theoretical basis for elucidating the relationship between ecological factors and genetic factors and their impact on the quality of medicinal materials. The results also have guiding significance for realizing the quality of medicinal materials.


Assuntos
Ginsenosídeos/química , Panax/química , Cromatografia Líquida de Alta Pressão , Regulação da Expressão Gênica de Plantas , Raízes de Plantas/química , Reação em Cadeia da Polimerase em Tempo Real , Saponinas/química
16.
Epigenetics Chromatin ; 11(1): 45, 2018 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-30103804

RESUMO

BACKGROUND: The product of dosage compensation in female mammals is the inactive X chromosome (Xi). Xi facultative heterochromatin is organized into two different types, one of which is defined by histone H3 trimethylated at lysine 9 (H3K9me3). The rationale for this study was to assess SET domain bifurcated 1 (SETDB1) as a candidate for maintaining this repressive modification at the human Xi. RESULTS: Here, we show that loss of SETDB1 does not result in large-scale H3K9me3 changes at the Xi, but unexpectedly we observed striking decompaction of the Xi territory. Close examination revealed a 0.5 Mb region of the Xi that transitioned from H3K9me3 heterochromatin to euchromatin within the 3' end of the IL1RAPL1 gene that is part of a common chromosome fragile site that is frequently deleted or rearranged in patients afflicted with intellectual disability and other neurological ailments. Centrally located within this interval is a powerful enhancer adjacent to an ERVL-MaLR element. In the absence of SETDB1, the enhancer is reactivated on the Xi coupled with bidirectional transcription from the ERVL-MaLR element. Xa deletion of the enhancer/ERVL-MaLR resulted in loss of full-length IL1RAPL1 transcript in cis, coupled with trans decompaction of the Xi chromosome territory, whereas Xi deletion increased detection of full-length IL1RAPL1 transcript in trans, but did not impact Xi compaction. CONCLUSIONS: These data support a critical role for SETDB1 in maintaining the ERVL-MaLR element and adjacent enhancer in the 3' end of the IL1RAPL1 gene in a silent state to facilitate Xi compaction.

17.
J Ginseng Res ; 42(3): 304-311, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29983611

RESUMO

Background: Ginseng black spot disease resulting from Alternaria panax Whuetz is a common soil-borne disease, with an annual incidence rate higher than 20-30%. In this study, the bacterial strains with good antagonistic effect against A. panax are screened. Methods: A total of 285 bacterial strains isolated from ginseng rhizosphere soils were screened using the Kirby-Bauer disk diffusion method and the Oxford cup plate assay. We analyzed the antifungal spectrum of SZ-22 by confronting incubation. To evaluate the efficacy of biocontrol against ginseng black spot and for growth promotion by SZ-22, we performed pot experiments in a plastic greenhouse. Taxonomic position of SZ-22 was identified using morphology, physiological, and biochemical characteristics, 16S ribosomal DNA, and gyrB sequences. Results: SZ-22 (which was identified as Brevundimonas terrae) showed the strongest inhibition rate against A. panax, which showed 83.70% inhibition, and it also provided broad-spectrum antifungal effects. The inhibition efficacies of the SZ-22 bacterial suspension against ginseng black spot reached 82.47% inhibition, which is significantly higher than that of the 25% suspension concentrate azoxystrobin fungicide treatment (p < 0.05). Moreover, the SZ-22 bacterial suspension also caused ginseng plant growth promotion as well as root enhancement. Conclusion: Although the results of the outdoor pot-culture method were influenced by the pathogen inoculum density, the cropping history of the field site, and the weather conditions, B. terrae SZ-22 controlled ginseng black spot and promoted ginseng growth successfully. This study provides resource for the biocontrol of ginseng black spot.

18.
Neuroimage ; 178: 445-460, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29802968

RESUMO

In recent years, machine learning approaches have been successfully applied to the field of neuroimaging for classification and regression tasks. However, many approaches do not give an intuitive relation between the raw features and the diagnosis. Therefore, they are difficult for clinicians to interpret. Moreover, most approaches treat the features extracted from the brain (for example, voxelwise gray matter concentration maps from brain MRI) as independent variables and ignore their spatial and anatomical relations. In this paper, we present a new Support Vector Machine (SVM)-based learning method for the classification of Alzheimer's disease (AD), which integrates spatial-anatomical information. In this way, spatial-neighbor features in the same anatomical region are encouraged to have similar weights in the SVM model. Secondly, we introduce a group lasso penalty to induce structure sparsity, which may help clinicians to assess the key regions involved in the disease. For solving this learning problem, we use an accelerated proximal gradient descent approach. We tested our method on the subset of ADNI data selected by Cuingnet et al. (2011) for Alzheimer's disease classification, as well as on an independent larger dataset from ADNI. Good classification performance is obtained for distinguishing cognitive normals (CN) vs. AD, as well as on distinguishing between various sub-types (e.g. CN vs. Mild Cognitive Impairment). The model trained on Cuignet's dataset for AD vs. CN classification was directly used without re-training to the independent larger dataset. Good performance was achieved, demonstrating the generalizability of the proposed methods. For all experiments, the classification results are comparable or better than the state-of-the-art, while the weight map more clearly indicates the key regions related to Alzheimer's disease.

19.
J Ophthalmol ; 2018: 1875627, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29670766

RESUMO

Background: To analyze the therapeutic effects of corneal debridement combined with intrastromal voriconazole in recalcitrant fungal keratitis. Methods: This is a retrospective study. Fourteen patients with recalcitrant fungal keratitis were treated by corneal debridement combined with intrastromal voriconazole (50 µg/0.1 ml). This paper reviews and analyzes the patients' basic state, surgical intervention, medicinal treatment, and outcomes. Results: The mean sizes of infiltration and ulcer were (5.54 ± 1.32)mm and (3.46 ± 1.03)mm, respectively, and the mean depth was (315.43 ± 57.72)µm. Twelve of the patients had satellite lesion, and 2 suffered hypopyon. After intrastromal voriconazole, the size of infiltration decreased significantly to (4.32 ± 1.10)mm (P < 0.001), but there was no significant change in ulcer size ((3.36 ± 0.92)mm, P = 0.082). Thirteen patients were cured after corneal debridement. The mean healing time was (15.38 ± 7.38) days. Excluding one cured patient with optic nerve atrophy and one patient for whom the treatment failed, the mean best-corrected visual acuity after healing was (0.23 ± 0.18)LogMAR, a significant improvement compared to pretreatment (0.87 ± 0.57(LogMAR), P = 0.01). The mean corneal astigmatism was (1.3 ± 1.6)D of 12 cured patients after healing and (1.0 ± 0.7)D at final follow-up, and there was no significant difference (P = 0.374). Conclusions: Corneal debridement combined with intrastromal voriconazole is a secure and effective treatment for recalcitrant fungal keratitis.

20.
A A Pract ; 11(3): 76-78, 2018 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-29634547

RESUMO

Median arcuate ligament syndrome (MALS), also known as celiac artery compression syndrome, is an uncommon condition classically characterized by chronic abdominal pain, weight loss, and abdominal bruit. Chronic mesenteric ischemia caused by intermittent compression of the celiac artery by the MAL provokes upper abdominal pain that is sympathetically mediated via the celiac plexus. Because it is a diagnosis of exclusion, diagnosis of MALS in the clinical setting is typically challenging. We present an atypical case which highlights the utility of celiac plexus block as both an assistant diagnostic tool and a predictor of surgical outcomes for suspected MALS.

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