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1.
World J Microbiol Biotechnol ; 35(12): 180, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31728755

RESUMO

Derived from RNA, 5'-ribonucleotides, especially Inosine-5'-monophosphate (IMP) and guanosine-5'-monophosphate (GMP), can enhance the umami taste of soy sauce. In this study, the RNA content of three different salt-tolerant yeasts was examined. The most valuable strain was subjected to atmospheric and room-temperature plasma (ARTP) mutagenesis, which improved its RNA content by 160.54%. Regular fermentation with RNA-enhanced strain failed to increase the amount of 5'-ribonucleotides in the soy sauce due to hydrolysis by phosphatase. A two-stage fermentation strategy was then carried out. Aroma compounds were mainly synthesized in the first stage, and RNA-enriched biomass was massively produced in the second stage followed by heat treatment to inactivate phosphatase. After the proposed strategy was applied, IMP and GMP in the soy sauce reached 68.54 and 89.37 mg/L, respectively. Moreover, the amounts of key aroma compounds and organic acids significantly increased. Results may provide new insights for improving the quality of soy sauce through microorganism breeding and fermentation control.


Assuntos
Mutagênese , Gases em Plasma , RNA , Tolerância ao Sal/genética , Tolerância ao Sal/efeitos da radiação , Alimentos de Soja , Zygosaccharomyces/genética , Zygosaccharomyces/efeitos da radiação , Cruzamento , Fermentação , Microbiologia de Alimentos , Cloreto de Sódio , Paladar , Temperatura Ambiente , Zygosaccharomyces/fisiologia
2.
Opt Lett ; 35(8): 1281-3, 2010 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-20410993

RESUMO

The optical trapping forces acting on a metallic Rayleigh particle are calculated for the case where a double-ring-shaped radially polarized beam is applied. The influence of the off-focus distance and the off-axis distance of a trapping particle on the trapping force is investigated. Compared with the use of the conventional single-ring-shaped radially polarized beam, the longer axial trap distance and the larger radial trap stiffness are predicted using a double-ring-shaped radially polarized beam in an optical trap. These features are useful for improving the trapping ability of an optical trap system where a longer axial trap distance is needed.

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