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J Forensic Sci ; 65(1): 84-89, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31454431


Impressions of friction ridges left on a surface are important evidence for identifying an individual and can be used to confirm his or her presence at the scene of an incident. Factors influencing the durability of fingerprints include the physiological characteristics and cleanliness of the individual, environmental factors, and time. The aim of the study was to determine the effect of atmospheric factors, gender, hand cleanliness and time on the width of friction ridge impressions and the amount of material forming the print. The research shows that factors such as gender, ambient conditions, and hand cleanliness affect the width of the ridge impressions and the quantity of material forming the print. The passage of time significantly reduces both the width of the ridge impressions and the quantity of the material forming the print.

Dermatoglifia , Higiene das Mãos , Manejo de Espécimes , Adulto , Escuridão , Exposição Ambiental , Feminino , Humanos , Luz , Masculino , Microscopia , Fatores Sexuais , Creme para a Pele , Temperatura , Fatores de Tempo , Adulto Jovem
J Therm Biol ; 84: 154-163, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31466748


L-proline (L-PROL) is an essential amino acid, a constituent of many proteins and the osmoprotective molecule produced and accumulated in higher plants and some freshwater microalgae in response to various environmental stressors. Knowledge on thermoprotective effects of this amino acid on freshwater invertebrates is very scarce. Therefore the aim of our study was to determine the effect of L-PROL at concentrations: 10 mg/L, 20 mg/L and 50 mg/L on swimming behavior (immobilization, swimming track density, swimming speed, turning ability) of Daphnia magna subjected to temperatures: 22 °C, 35 °C and 38 °C. We found that L-PROL elevated all the measured swimming parameters at 22 °C when compared to the untreated crustaceans. Furthermore, L-PROL alleviated heat-induced inhibition of these parameters in the experimental animals subjected to 35 °C. The results suggest that L-PROL stimulates swimming performance and alleviates alterations of swimming parameters induced by heat stress in D. magna. Moreover, these findings may support the hypothesis that in natural conditions, L-PROL may protect crustaceans against thermal stress.

Daphnia/efeitos dos fármacos , Resposta ao Choque Térmico/efeitos dos fármacos , Prolina/farmacologia , Natação , Animais , Comportamento Animal/efeitos dos fármacos , Daphnia/fisiologia , Feminino , Resposta ao Choque Térmico/fisiologia
Arh Hig Rada Toksikol ; 70(1): 14-17, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30956217


Palythoa spp. corals and some other marine organisms contain one of the most poisonous substances ever known - palytoxin (PTX). Due to their modest life requirements and ease of breeding, these corals are popular in home aquariums. Here we refer to a case of PTX poisoning of a middle-aged woman who inhaled poisonous vapours while brushing the corals from live rock and compare it with the available literature. As the case revealed that the symptoms of PTX poisoning are not specific and neither is treatment, our aim was to give a brief tabulated review of the symptoms that may indicate such poisoning. Cases of palytoxin poisoning have been reported worldwide, and severe ones (mostly due to ingestion of contaminated sea food) can end in death. As it appears, most (if not all) poisonings result from unawareness of the risk and reckless handling by aquarists. This is one of the first articles which provides some practical advice about the use of personal protection equipment, including gloves, masks, eyewear, and other clothing during any coral manipulation to minimise the risk. We also draw attention to the lack of marketing/trading regulations for dangerous coral species and/or regulations or instructions dealing with their removal and health protection.

Acrilamidas/envenenamento , Poluentes Atmosféricos/envenenamento , Antozoários/química , Venenos de Cnidários/envenenamento , Exposição Ambiental/efeitos adversos , Exposição por Inalação/efeitos adversos , Água do Mar/química , Poluentes da Água/envenenamento , Adulto , Animais , Feminino , Humanos
J Forensic Sci ; 64(3): 711-716, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30577070


The aim of the study was to determine the effect of gender, body mass index, and time on the width of friction ridge impressions in fingerprints and the content of sweat and sebum forming the print. The research showed that the friction ridge impressions of women are significantly wider than those of men, and this tendency persists throughout the existence of the print. The differences may result from the anatomical structure of the skin, as well as from the use of protective products such as creams, which are used more often by women. The width of friction ridge impression is similar in underweight and overweight people, but greater than in those with normal body weight, and the amount of sweat and sebum increases with body weight. The passage of time significantly reduces both the width of the friction ridge impression and the content of sweat and sebum secretions in the print.

Dermatoglifia , Adulto , Índice de Massa Corporal , Feminino , Humanos , Modelos Lineares , Masculino , Microscopia , Sebo/fisiologia , Fatores Sexuais , Suor/fisiologia , Fatores de Tempo , Adulto Jovem
J Comp Physiol B ; 188(5): 779-791, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29948158


Ectoine (ECT) is a compatible solute synthesized mostly by halophilic microorganisms subjected to various stressful factors. Its protective properties in bacteria and some populations of isolated cells subjected to different stressors are reported; however, little is known on its effects against a commonly used compound, ethanol (ETH). The purpose of our study was to determine the effects of ETH alone (at 20 and 60 g/L) and in the combination with various concentrations of ECT (5, 10, and 25 mg/L) at various times of exposure on behavioural, physiological, and biochemical parameters of a model invertebrate Daphnia magna. In the present study, we determined the following parameters: immobilisation, heart rate, thoracic limb movement, catalase (CAT) activity, and nitric oxide species (NOx) level. Our study revealed that both concentrations of ETH alone induced immobilisation and decrease of swimming velocity, heart rate, and thoracic limb activity; however, catalase activity and NOx levels were increased. On the other hand, the animals exposed to the combinations of ETH + ECT showed a reduced immobilisation and alleviated inhibition of heart rate and thoracic limb activity, lower increase of CAT activity, and NOx level when compared to the crustaceans subjected to ETH alone. The most distinct alleviation of toxic effects was noted in the combinations in which the highest concentration of ECT were used. The results suggest that ETH may induce oxidative stress in daphnids and attenuating effects of ECT probably result from its antioxidative properties.

Diamino Aminoácidos/farmacologia , Daphnia/efeitos dos fármacos , Etanol/toxicidade , Substâncias Protetoras/farmacologia , Animais , Catalase/metabolismo , Daphnia/fisiologia , Extremidades/fisiologia , Frequência Cardíaca/efeitos dos fármacos , Atividade Motora/efeitos dos fármacos , Óxido Nítrico/metabolismo