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1.
Ideggyogy Sz ; 72(7-8): 264-272, 2019 Jul 30.
Artigo em Húngaro | MEDLINE | ID: mdl-31517459

RESUMO

Background and purpose: Objective - Our aim is to evaluate sleep habits, sleep quality and influencing factors among preschool- and schoolchildren. Methods: Two questionnaires were recorded. Questionnaire 1 dealt with sleeping habits, breastfeeding and health behavior of preschool children and infant, and it contained the abbreviated version of the Children's Sleep Habits Questionnaire. Questionnaire 2 dealt with health behavior and the application of sleep hygiene rules, as well as it contained the Athens Insomnia Scale. Subjects - We assessed a total of 1063 questionnaires: 516 kindergarten children participated in our online survey across the country; 547 primary and secondary school students participated in the 2nd questionnaire survey in Szolnok. Results: Parents' observation shows that the average nighttime sleeping time of kindergarten children is 10 hours 20 minutes on weekdays and 10 hours 36 minutes on weekends. The most popular sleeping habits in kindergarten age: teal reading (65.1%) and co-sleeping (42.8%). Parents of infants used breastfeeding (50.4%) and rocking (43.2%) most frequently before sleep. Co-sleeping has a positive influence on the length of lactation. Among the preschool sleeping habits we have proved a number of positive effects of teal reading, while watching television have negative effects. The sleep quality of school-age children according to the Athens Insomnia Scale is 6.11 points (SD: 4.11), 19% of the children are insomniac. Their sleep time is 7 hours 31 minutes on weekdays and 9 hours 30 minutes on weekends. The usage of good health behavior and sleep hygiene rules positively influence sleep quality and sleep duration. Conclusion: With our results, we would like to draw the attention of children and parents to the importance of sleeping and using sleep hygiene rules.


Assuntos
Hábitos , Comportamentos Relacionados com a Saúde , Higiene do Sono , Sono/fisiologia , Televisão , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Iluminação , Masculino , Instituições Acadêmicas , Inquéritos e Questionários
2.
J Dairy Sci ; 102(11): 9570-9585, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31477303

RESUMO

Colitis severely affects the quality of life of patients, and lactic acid bacteria have been reported to be able to improve or treat colitis. In this study, we selected a strain of Lactobacillus fermentum (CQPC04) with good resistance in vitro to evaluate its effect on improvement in mice with dextran sulfate sodium (DSS)-induced colitis. We analyzed the effects of L. fermentum CQPC04 on mice with colitis macroscopically via colon length and histopathology. We also used conventional biochemical and ELISA kits, real-time quantitative PCR (RT-qPCR), and Western blotting to analyze microscopically the effects of L. fermentum CQPC04 on related oxidant indices and pro- and anti-inflammatory cytokines in serum and colon tissue of mice. The results indicated that L. fermentum CQPC04 notably increased colon length and ameliorated pathological damage of colon tissue in colitic mice. Serum indices showed that L. fermentum CQPC04 increased the enzyme activity of total superoxide dismutase (T-SOD) and catalase (CAT) and decreased the content of malondialdehyde (MDA) and the activity of myeloperoxidase (MPO). In addition, it inhibited the release of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), IFN-γ, IL-1ß, IL-6, and IL-12, and increased the release of the anti-inflammatory cytokine IL-10 in serum. The RT-qPCR experiments confirmed that L. fermentum CQPC04 downregulated the expression of pro-inflammatory cytokine nuclear factor-κB-p65 (NF-κBp65), NF-κB inhibitor-α (IκB-α), TNF-α, IFN-γ, IL-1ß, IL-6, cyclooxygenase 2 (COX-2), and inducible nitric oxide synthase (iNOS), and upregulated the expression of IL-10 in colon tissue. Western blot analysis indicated that L. fermentum CQPC04 significantly reduced expression of NF-κBp65, TNF-α, IL-1ß, COX-2, and iNOS in mouse colon tissues, and increased expression of IκB-α and superoxide dismutase 2 (SOD2). Thus, L. fermentum CQPC04 could effectively alleviate the symptoms of DSS-induced colitis mice and is a potential probiotic for human experiments.


Assuntos
Colite/dietoterapia , Lactobacillus fermentum , NF-kappa B/metabolismo , Probióticos/uso terapêutico , Transdução de Sinais/efeitos dos fármacos , Animais , Colite/induzido quimicamente , Colite/metabolismo , Colite/prevenção & controle , Citocinas/sangue , Sulfato de Dextrana , Feminino , Camundongos , Camundongos Endogâmicos ICR , Óxido Nítrico Sintase Tipo II/metabolismo , Substâncias Protetoras , Fator de Necrose Tumoral alfa/metabolismo
3.
J Sci Food Agric ; 97(3): 1010-1017, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27247268

RESUMO

BACKGROUND: In soybean, at least 16 seed proteins have been identified as causing allergenic reactions in sensitive individuals. As a soybean genebank accession low in the immunodominant protein P34 (Gly m Bd 30K) has recently been found, introgression of the low-P34 trait into adapted soybean germplasm has been attempted in order to improve the safety of food products containing soybean protein. Therefore, marker-assisted selection and proteomics were applied to identify and characterize low-P34 soybeans. RESULTS: In low-P34 lines selected from a cross-population, concentrations of the P34 protein as identified with a polyclonal antibody were reduced by 50-70% as compared to P34-containing controls. Using 2D electrophoresis and immunoblotting, the reduction of P34 protein was verified in low-P34 lines. This result was confirmed by liquid chromatographic-tandem mass spectrometric analysis, which revealed either a reduction or complete absence of the authentic P34 protein as suggested from presence or absence of a unique peptide useful for discriminating between conventional and low-P34 lines. CONCLUSION: Marker-assisted selection proved useful for identifying low-P34 soybean lines for the development of hypoallergenic soy foods. The status of the P34 protein in low-P34 lines needs further characterization. In addition, the food safety relevance of low-P34 soybeans should be tested in clinical studies. © 2016 Society of Chemical Industry.


Assuntos
Antígenos de Plantas/efeitos adversos , Cruzamentos Genéticos , Regulação para Baixo , Melhoramento Vegetal , Sementes/química , Alimentos de Soja/análise , Proteínas de Soja/efeitos adversos , Soja/química , Antígenos de Plantas/genética , Antígenos de Plantas/metabolismo , Ensaio de Imunoadsorção Enzimática , Sistemas Especialistas , Hipersensibilidade Alimentar/dietoterapia , Hipersensibilidade Alimentar/prevenção & controle , Inocuidade dos Alimentos , Alimento Funcional/efeitos adversos , Alimento Funcional/análise , Regulação da Expressão Gênica de Plantas , Ligação Genética , Marcadores Genéticos , Humanos , Repetições de Microssatélites , Proteômica/métodos , Sementes/efeitos adversos , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Seleção Genética , Alimentos de Soja/efeitos adversos , Proteínas de Soja/genética , Proteínas de Soja/metabolismo , Soja/efeitos adversos , Soja/crescimento & desenvolvimento , Soja/metabolismo
4.
Eur Arch Psychiatry Clin Neurosci ; 262(8): 687-96, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22526731

RESUMO

Nightmares are intense, emotionally negative mental experiences that usually occur during late-night sleep and result in abrupt awakenings. Questionnaire-based studies have shown that nightmares are related to impaired sleep quality; however, the polysomnographic profile of nightmare subjects has been only scarcely investigated. We investigated the sleep architecture of 17 individuals with frequent nightmares and 23 control subjects based on polysomnographic recordings of a second night spent in the laboratory after an adaptation night. Nightmare subjects in comparison with control subjects were characterized by impaired sleep architecture, as reflected by reduced sleep efficiency, increased wakefulness, a reduced amount of slow wave sleep, and increased nocturnal awakenings, especially from Stage 2 sleep. While these differences were independent of the effects of waking psychopathology, nightmare subjects also exhibited longer durations of REM sleep that was mediated by heightened negative affect. Our results support that nightmares are related to altered sleep architecture, showing impaired sleep continuity and emotion-related increase in REM propensity.


Assuntos
Sonhos/psicologia , Fases do Sono/fisiologia , Transtornos do Sono-Vigília/fisiopatologia , Adolescente , Ansiedade/diagnóstico , Ansiedade/etiologia , Eletroencefalografia , Feminino , Humanos , Masculino , Análise Multivariada , Polissonografia , Escalas de Graduação Psiquiátrica , Psicometria , Transtornos do Sono-Vigília/classificação , Inquéritos e Questionários , Vigília/fisiologia , Adulto Jovem
5.
J Basic Microbiol ; 47(1): 74-83, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17304622

RESUMO

The objective of this study was to investigate the role of the Pap1 transcription factor in response to long-term Cd(2+) stress. The Schizosaccharomyces pombe wild-type strain and the Deltapap1 mutant, treated with 0.5 mM CdSO(4), were used in antioxidant enzyme and gene expression experiments. The Deltapap1 mutant proved to be sensitive to Cd(2+) in the spot test assay, suggesting that the Pap1 transcription factor plays an important role in the response to Cd(2+) stress. The Cd(2+) uptake was the same in both strains. Determination of the superoxide level in the wild-type strain proved that superoxide was generated, suggesting that long-term Cd(2+) treatment could trigger oxidative stress. Furthermore, the Deltapap1 mutant displayed higher amounts of superoxide. These results were supported by the significantly lower amount of peroxide generated in the reaction catalyzed by superoxide dismutase (SOD). The Deltapap1 mutant had a significantly lower glutathione S-transferase specific activity than that of the wild-type strain during long-term Cd(2+) stress, caused by the lower GSH and sulfide assimilation. We have demonstrated that GST III activity was not induced by Cd(2+) stress in the Deltapap1 mutant. The overall low GST activity was not sufficient for the cell to eliminate Cd(2+) caused damage and could result in a Cd(2+)-sensitive phenotype of the Deltapap1 mutant. The RT-PCR and Northern blot experiments proved that gst2 was not induced either by short-term or by long-term Cd(2+) treatment. The SPCC965.06 (a putative K(+) ion channel subunit) gene expression increased, while the hmt1 (an ABC-type vacuolar transporter protein) expression decreased in both strains. No detectable alteration in the mRNA levels of, gpx1, hmt2, sod1, sod, and trx1 was observed. SOD enzyme analyses revealed that the absence of Pap1 protein could result in a lower SODs activity and affect the sulfate assimilation. This is the first report on the fact that the Pap1 transcription factor could play an important role in the cellular post-transcriptional/post-translational enzyme activity induction processes of SODs that occur in response to Cd(2+).


Assuntos
Fatores de Transcrição de Zíper de Leucina Básica/genética , Cádmio/farmacologia , Glutationa Transferase/metabolismo , Proteínas de Schizosaccharomyces pombe/genética , Schizosaccharomyces/enzimologia , Schizosaccharomyces/genética , Superóxido Dismutase/metabolismo , Fatores de Transcrição de Zíper de Leucina Básica/metabolismo , Regulação Fúngica da Expressão Gênica , Peróxido de Hidrogênio/metabolismo , Modelos Biológicos , Mutação , Proteínas Associadas a Pancreatite , Schizosaccharomyces/metabolismo , Proteínas de Schizosaccharomyces pombe/metabolismo , Superóxidos/metabolismo , Fatores de Tempo
6.
FEMS Yeast Res ; 6(8): 1140-8, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17156011

RESUMO

Lovastatin inhibited the growth of Candida albicans in a fungistatic way. Although it triggers apoptosis in a great variety of eukaryotic cells, including many tumour cell lines, lovastatin failed to provoke apoptotic events in this human pathogen. The fungistatic behaviour of this statin might arise from its negative influence on membrane fluidity. Because yeast-->pseudomycelium and hyphae morphogenetic transitions took place under exposure to lovastatin morphogenetic switch and apoptotic cell death must be regulated independently in C. albicans.


Assuntos
Antifúngicos/farmacologia , Apoptose/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Lovastatina/farmacologia , Candida albicans/crescimento & desenvolvimento , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Fragmentação do DNA/efeitos dos fármacos , DNA Fúngico/efeitos dos fármacos , Humanos
7.
J Basic Microbiol ; 43(2): 96-103, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-12746851

RESUMO

The stable Cr(VI)-tolerant chr1-66T mutant of Schizosaccharomyces pombe, which carries one simple gene mutation responsible for Cr(VI) tolerance, accumulated and reduced the chromate anion (CrO(4)(2-)) significantly more slowly than did its parental strain 6chr(+). The mutant chr1-66T proved to be sensitive to oxidative stressors such as H(2)O(2), menadione, tert-butyl hydroperoxide and Cd(2+). Both the Cr(VI) tolerance and the oxidative stress sensitivity were attributed to a decreased specific glutathione reductase activity. These effects were also enhanced with a decrease in the specific mitochondrial Mn-SOD activity.


Assuntos
Cromatos/farmacologia , Glutationa Redutase/metabolismo , Radical Hidroxila/metabolismo , Schizosaccharomyces/efeitos dos fármacos , Farmacorresistência Fúngica , Schizosaccharomyces/metabolismo
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