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2.
China CDC Wkly ; 3(19): 414-415, 2021 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-34594897
3.
Front Vet Sci ; 8: 714249, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660760

RESUMO

Enterocytozoon bieneusi is a microsporidian and zoonotic species. This study investigated the prevalence and distribution of E. bieneusi genotypes in farmed masked palm civets using nested PCR, as well as assessed their zoonotic potential by phylogenetic analysis of the ITS region of the rRNA region. Here, we collected 251 fecal specimens from farmed masked palm civets (Paguma larvata) from the Hainan Island, China. In total, 128 of 251 samples were positive for E. bieneusi, with an average infection rate of 51.0%. Seventeen genotypes were identified including 12 known genotypes-HNR-VI (n = 56), SHR1 (n = 45), SHW7 (n = 6), KIN-1 (n = 3), D (n = 3), New1 (n = 3), EbpC (n = 2), CHC5 (n = 1), CHG19 (n = 1), CHN4 (n = 1), EbpA (n = 1), and Henan-III (n = 1)-and five novel genotypes (HNPL-I to HNPL-II; one each). Phylogenetic analysis categorized these genotypes into two groups. Thirteen of them were members of the zoonotic group 1, and the remaining four genotypes were in group 12. This study has shown that the infection rates of E. bieneusi in masked palm civets from Hainan were relatively high and provide baseline data to control and prevent microsporidiosis in farm-related communities. Therefore, infections in masked palm civets with zoonotic genotypes D, EbpC, CHN4, EbpA, KIN-1, and Henan-III should be considered potential threats to public health.

4.
Sci Total Environ ; : 151039, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34673063

RESUMO

The adsorption/desorption of arsenic (As) in agricultural soils is of utmost importance for the evaluation of its kinetic release and potential of entering the food chain by uptake of crops. However, the mobility of As in soils is closely related to the migration behavior of soil phosphorus (P) due to their chemical similarity. Here, the distribution and desorption kinetics of As and P in four different types of farmland soils were simultaneously estimated by cerium oxide-based diffusive gradients in thin films technique (CeO2-DGT) coupled with dynamic model of DGT induced fluxes in soils (DIFS). CeO2-DGT was deployed in the soils over 400 h to investigate the interactions between As and P for their migration behaviors. The accumulated masses of As in the DGT devices showed reverse orders with those of P among the four soils, indicating their competitive adsorption on soil solids. The distribution coefficients (Kdl) for the labile As and P derived from the DIFS model were mutually exclusive. Clay in the soil reduced the pool size of the labile As by increasing the irreversible adsorption of As on soil particles. The adsorption rate constants of As were much smaller than P but their desorption rate constants were comparable. Among the four soils, the soil with the highest soil labile As/P molar ratio measured by DGT showed the largest potential of As phytotoxicity. Both As and P could reach the equilibrium of resupply within 0.7- 18 min under DGT depletion, and significant negative correlation was observed between the desorption rate (kb) of As and clay content in the soils.

5.
Sci Total Environ ; 807(Pt 2): 150519, 2021 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-34610409

RESUMO

The urbanization and development of Tibetan Plateau (TP) probably results in a significant contamination of organic pollutants, such as organophosphate flame retardants (OPFRs). However, there is a lack of monitoring and evaluation of their occurrence and risks in the soil of TP. We investigated the concentrations, vertical distributions, potential sources, and ecological risks of OPFRs in soil profiles from four regions of TP, China. The total concentrations of OPFRs in all soil samples ranged from 1.35 to 126 ng/g with a median of 12.6 ng/g. Relatively high concentrations were discovered in the top soils from Lhasa, suggesting a rising contamination around cities of TP due to anthropogenic disturbance. Tri-n-butyl phosphate (TNBP) was the dominant OPFRs followed by tris(2-chloroethyl) phosphate (TCEP). Vertical distribution of ΣOPFRs was discovered, especially at site Lhasa. Source apportionment based on principle component analysis and correlation analysis suggests that OPFRs in the TP soil mainly originate from atmospheric transport, while some OPFRs in the top soil may be also influenced by nearby sources. The vertical distributions of OPFRs in soil may be influenced by both soil and chemical properties, as well as their use. The ecological risk quotients (RQs) of 6 OPFRs in the TP soil were calculated, and most of their ecological risks were relatively low or negligible. However, for the worst-case scenario calculated by the 95th percentile concentrations, TNBP and tris(2-chloro-isopropyl) phosphate (TCIPP) at site Lhasa and cresyl diphenyl phosphate (CDP) at site Nagri had moderate risks. More attentions should be paid to the Tibetan Plateau in the future due to the rising ecological risks of OPFRs, especially to the areas around cities.

7.
Micromachines (Basel) ; 12(8)2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34442516

RESUMO

A quartz crystal resonator (QCR) is an indispensable electronic component in the field of the modern electronics industry. By designing and depositing electrodes of different shapes and thicknesses on a quartz wafer with a certain fundamental frequency, the desired target frequency can be obtained. Affected by factors such as the deposition equipment, mask, wafer size and placement position, it is difficult to accurately obtain the target frequency at a given time, especially for mass-produced QCRs. In this work, a laser with a wavelength of 532 nm was used to thin the electrodes of a QCR with a fundamental frequency of 10 MHz. The electrode surface was etched through a preset processing pattern to form a processing method of local thinning of the electrode surface. At the same time, the effect of laser etching on silicon dioxide and resonator performance was analyzed. Satisfactory trimming frequency-increasing results were achieved, such as a frequency modulation accuracy of 1 ppm, frequency distribution with good consistency and equivalent parameters with small changes, by the laser partial etching of the resonator electrode. However, when the surface electrode was etched into using through-holes, the attenuation amplitude of the equivalent parameter became larger, especially in terms of the quality factor (Q), which decreased from 63 K to 1 K, and some resonators which had a serious frequency drift of >40%. In this case, a certain number of QCRs were no longer excited to vibrate, which was due to the disappearance of the piezoelectric effect caused by the local thermal phase change in the quartz wafer.

9.
J Inflamm Res ; 14: 3039-3047, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34262327

RESUMO

Objective: This study explored the correlation between myocardial infarction (MI) and the Glu504Lys polymorphism in the aldehyde dehydrogenase 2 (ALDH2) gene in the Qingyuan area. Methods: The Glu504Lys polymorphism of the ALDH2 gene was analyzed using the polymerase chain reaction and deoxyribonucleic acid microarray analysis for 468 patients diagnosed with MI for the first time and 132 healthy subjects. Results: There was a significant difference in the distribution of the ALDH2 genotype between the MI group and the control group (P = 0.0492), but there was no significant difference in allele frequency between the two groups (P = 0.1363). The clinical data showed that there were statistically significant differences (P < 0.05) in the two groups' gender and age distributions, rates of diabetes and hypertension, levels of alcohol and tobacco use, serological levels of heart markers, blood lipids and glucose. The subgroup analysis of ALDH2 genotypes found that alcohol consumption, high levels of myoglobin, and low levels of high-density lipoprotein cholesterol were significantly associated with a higher incidence of MI (P < 0.05). After adjusting for gender, hypertension, diabetes, and other related influencing factors, logistic regression analysis showed that the ALDH2 genotype GA/AA was an independent risk factor for MI (P < 0.05, OR = 1.479, 95% CI = 1.003-2.179). Conclusion: The presence of risk alleles with the genetic effect (ALDH2 genotype GA/AA) is an independent risk factor for MI.

10.
Environ Pollut ; 284: 117532, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34261226

RESUMO

We investigated the concentration, composition, and potential risk of organophosphate esters (OPEs) and phthalates (PAEs) in soils and vegetation from rural areas of Dalian, Northeast China. The residues of total OPEs and PAEs in soils were in the range of 33.1-136 ng/g dw (dry weight) and 465-5450 ng/g dw, while the values in plants were 140-2360 ng/g dw and 2440-21800 ng/g dw, respectively. The concentrations of both chemicals in the plant rhizosphere soils were significantly lower than those in the bulk soils, suggesting an enhanced degradation or uptake by plant. The contaminations in soils also varied for different land use types with the concentrations generally higher in paddy soils than those in maize soils. The OPE and PAE concentrations in plant leaves were slightly higher than those in their corresponding roots. The bioconcentration factors of OPEs & PAEs were significantly negatively correlated with their octanol-water partition coefficients. A hazard assessment suggested potential medium to high risks from tricresyl phosphate (TMPP) and di-n-butyl phthalate (DNBP) for the agricultural soils in Dalian of China. Although the ecological risks of OPEs and PAEs in the rhizosphere soils were lower than those in the bulk soils, the relevant risk could still endanger human health via oral intake of these plants. The daily dietary intakes of OPEs and PAEs via vegetable and rice consuming were estimated, and the result suggests a higher exposure risk via ingestion of leafy vegetable than rice.


Assuntos
Ácidos Ftálicos , Solo , China , Monitoramento Ambiental , Ésteres , Humanos , Organofosfatos , Medição de Risco
11.
BMC Vet Res ; 17(1): 213, 2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34107958

RESUMO

BACKGROUND: Enterocytozoon bieneusi, a microsporidian species, is a zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored the different genotypes of E. bieneusi in wild rhesus macaques (Macaca mulatta) (Hainan Island of China), and assessed their zoonotic potential. METHODS: We collected 173 fecal specimens from wild rhesus macaques living in Nanwan Monkey Island, Hainan, China. Subsequently, we identified and genotyped E. bieneusi using nested PCR analysis amplification of the internal transcribed spacer region (ITS) of the rRNA gene. Lastly, a neighbor-joining tree was built based on gene sequences from the ITS region of E. bieneusi. RESULTS: Of the 173 specimens from wild rhesus macaques, 26 (15%) were infected with E. bieneusi. We identified six genotypes of E. bieneusi, of which five were known: PigEBITS7 (n = 20), D (n = 2), Type IV (n = 1), Peru6 (n = 1), Henan-III (n = 1), and a novel genotype: HNM-IX (n = 1). From the phylogenetic analysis, the six genotypes identified here were all clustered into zoonotic group 1. CONCLUSION: This study is the first report to detect E. bieneusi infection in wild rhesus macaques from Hainan, China. Human-pathogenic genotypes D, Henan-III, Peru6, PigEbITS7, and Type IV in the wild rhesus macaques support these animals infected with E. bieneusi have a public health significance.


Assuntos
Enterocytozoon/genética , Macaca mulatta/virologia , Microsporidiose/veterinária , Doenças dos Macacos/virologia , Animais , Animais Selvagens , China/epidemiologia , Enterocytozoon/isolamento & purificação , Feminino , Genoma Viral , Genótipo , Humanos , Incidência , Masculino , Microsporidiose/epidemiologia , Microsporidiose/virologia , Doenças dos Macacos/epidemiologia , Filogenia , Prevalência , Saúde Pública , Zoonoses/virologia
12.
J Cell Physiol ; 2021 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-34121193

RESUMO

In a healthy body, reactive oxygen species (ROS) and antioxidants remain balanced. When the balance is broken toward an overabundance of ROS, oxidative stress appears and may lead to oocyte aging. Oocyte aging is mainly reflected as the gradual decrease of oocyte quantity and quality. Here, we aim to review the relationship between oxidative stress and oocyte aging. First, we introduced that the defective mitochondria, the age-related ovarian aging, the repeated ovulation, and the high-oxygen environment were the ovarian sources of ROS in vivo and in vitro. And we also introduced other sources of ROS accumulation in ovaries, such as overweight and unhealthy lifestyles. Then, we figured that oxidative stress may act as the "initiator" for oocyte aging and reproductive pathology, which specifically causes follicular abnormally atresia, abnormal meiosis, lower fertilization rate, delayed embryonic development, and reproductive disease, including polycystic ovary syndrome and ovary endometriosis cyst. Finally, we discussed current strategies for delaying oocyte aging. We introduced three autophagy antioxidant pathways like Beclin-VPS34-Atg14, adenosine 5'-monophosphate (AMP)-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR), and p62-Keap1-Nrf2. And we also describe the different antioxidants used to combat oocyte aging. In addition, the hypoxic (5% O2 ) culture environment for oocytes avoiding oxidative stress in vitro. So, this review not only contribute to our general understanding of oxidative stress and oocyte aging but also lay the foundations for the therapies to treat premature ovarian failure and oocyte aging in women.

13.
Plant Cell Physiol ; 62(9): 1409-1422, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34185870

RESUMO

Previous studies had demonstrated that in Arabidopsis, IDM3 is involved in ROS1-mediated DNA demethylation pathway, and SUVH-SDJ complex functions as a DNA methylation reader complex for enhancing gene transcription, which presumably recruits ROS1 to the promoters of target genes for DNA demethylation. Here, our analyses, however, showed that the IDM3 and SDJ1/2/3, the components of the SUVH-SDJ complex, are implicated in establishing and/or maintaining DNA methylation as well through DDR (DRD1-DMS3-RDM1) complex. idm3-3 or sdj1/2/3 mutations led to genome-wide DNA hypomethylation, and both mutants shared a large number of common hypo-DMRs (Differentially Methylated Regions) with rdm1-4 and dms3-4, suggesting that IDM3 and SDJ1/2/3 help establish and/or maintain DNA methylation, mediated by RdDM pathway, at a subset of genomic regions largely through DDR complex. IDM3 is able to strongly interact with RDM1 and DMS3, but weakly with SDJ1 and SDJ3; SDJ1 and SDJ3 is capable of interacting separately with RDM1 and DMS3. Furthermore, comparisons of DNA methylation features in idm3-3 and sdj1/2/3 indicated that idm3-3 and sdj1/2/3 mutations make differential impacts on DNA methylation levels and patterns on a genome-wide scale, indicating that they are targeted to quite distinct genomic regions for aiding in DNA methylation. Further analyses on ChIP-seq data demonstrated that RDM1, DMS3 and NRPE1 are enriched in IDM3- and SDJ1/2/3-targted regions. Altogether, our results provide clear demonstration that IDM3 and SDJ1/2/3 play a part in establishing and/or maintaining DNA methylation of a group of genomic regions, through the DDR complex.

14.
Biosens Bioelectron ; 184: 113220, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33878592

RESUMO

A hybrid recognition interface combining peptide and molecularly imprinted polymer (MIP) was achieved by introducing a vancomycin binding tripeptide in the preparation of MIP to implement high affinity and specificity recognition of vancomycin in complex matrices. The tripeptide that can specifically bind vancomycin was immobilized onto gold nanoparticles (GNPs) deposited on a glassy carbon electrode (GCE) by Au-S bond, and then a controlled electropolymerization of dopamine was carried out to imprint the vancomycin-peptide complex. After removing vancomycin from the polydopamine (PDA), hybrid peptide-MIP cavities containing multiple binding sites for vancomycin in the MIPDA/peptide/GNPs/GCE were obtained. The electrode had better selectivity and higher sensitivity toward vancomycin than either peptide or MIP modified GNPs/GCE, and the limit of quantification was as low as 10 pM by electrochemical impedance spectroscopy. The real samples, including fetal calf serum, probiotic drink and honey spiked with 0.17-2.0 µM vancomycin were analyzed on the MIPDA/peptide/GNPs/GCE, with the recoveries of 92.16-104.67%. The present study provides a sensitive, reliable method for the detection of vancomycin in complex matrices.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Impressão Molecular , Técnicas Eletroquímicas , Eletrodos , Ouro , Limite de Detecção , Polímeros Molecularmente Impressos , Vancomicina
15.
Plant Cell Physiol ; 62(7): 1168-1184, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33836080

RESUMO

To discover new mutants conferring enhanced tolerance to drought stress, we screened a mutagenized upland rice (Oryza sativa) population (cv. IAPAR9) and identified a mutant, named idr1-1 (increased drought resistance 1-1), with obviously increased drought tolerance under upland field conditions. The idr1-1 mutant possessed a significantly enhanced ability to tolerate high-drought stresses. Map-based cloning revealed that the gene LOC_Os05g26890, residing in the mapping region of IDR1 locus, carried a single-base deletion in the idr1-1 mutant. IDR1 encodes the Gα subunit of the heterotrimeric G protein (also known as RGA1), and this protein was localized in nucleus and to plasma membrane or cell periphery. Further investigations indicated that the significantly increased drought tolerance in idr1-1 mutants stemmed from a range of physiological and morphological changes, including greater leaf potentials, increased proline contents, heightened leaf thickness and upregulation of antioxidant-synthesizing and drought-induced genes, under drought-stressed conditions. Especially, reactive oxygen species (ROS) production might be remarkably impaired, while ROS-scavenging ability appeared to be markedly enhanced due to significantly elevated expression of ROS-scavenging enzyme genes in idr1-1 mutants under drought-stressed conditions. In addition, idr1-1 mutants showed reduced expression of OsBRD1. Altogether, these results suggest that mutation of IDR1 leads to alterations in multiple layers of regulations, which ultimately leads to changes in the physiological and morphological traits and limiting of ROS levels, and thereby confers obviously increased drought tolerance to the idr1-1 mutant.

16.
J Thorac Dis ; 13(3): 1315-1326, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33841925

RESUMO

Background: The purpose of this study was to explore the prognostic factors of oesophageal signet ring cell (SRC) carcinoma and to construct a nomogram for predicting the outcome of SRC carcinoma of oesophagus. Methods: A total of 968 cases of oesophageal SRC carcinoma were extracted from the Surveillance, Epidemiology, and End Results (SEER) database between 2004 and 2016. Cases were divided into training cohort and validation cohort. Univariate and multivariable Cox analyses was performed to select the predictors of overall survival (OS for the nomogram. The performance of nomogram was validated with Harrell's concordance index (C-index), calibration curves and decision curve analysis (DCA). Results: The 1- and 5-year OS in the training cohort were 0.446 and 0.146, respectively, and the 1- and 5-year OS in the validation cohort were 0.459 and 0.138. The independent prognostic factors for establishing the nomogram were marital status, invasion of the surrounding tissue, lymph node metastasis, distant metastasis, surgery and chemotherapy. The Harrell's c-index value of the training cohort and validation cohort were 0.723 and 0.708. In the calibration curves, the predicted survival probability and the actual survival probability have a considerable consistency. DCA indicated the favourable potential clinical utility of the nomogram. Conclusions: A nomogram to predict the OS of patients with oesophageal SRC carcinoma was established. The validation of the nomogram fully demonstrates its great performance.

18.
Thorac Cancer ; 12(9): 1469-1488, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33787090

RESUMO

Perioperative adjuvant treatment has become an increasingly important aspect of the management of patients with non-small cell lung cancer (NSCLC). In particular, the success of immune checkpoint inhibitors, such as antibodies against PD-1 and PD-L1, in patients with lung cancer has increased our expectations for the success of these therapeutics as neoadjuvant immunotherapy. Neoadjuvant therapy is widely used in patients with resectable stage IIIA NSCLC and can reduce primary tumor and lymph node stage, improve the complete resection rate, and eliminate microsatellite foci; however, complete pathological response is rare. Moreover, because the clinical benefit of neoadjuvant therapy is not obvious and may complicate surgery, it has not yet entered the mainstream of clinical treatment. Small-scale clinical studies performed in recent years have shown improvements in the major pathological remission rate after neoadjuvant therapy, suggesting that it will soon become an important part of NSCLC treatment. Nevertheless, neoadjuvant immunotherapy may be accompanied by serious adverse reactions that lead to delay or cancellation of surgery, additional illness, and even death, and have therefore attracted much attention. In this article, we draw on several sources of information, including (i) guidelines on adverse reactions related to immune checkpoint inhibitors, (ii) published data from large-scale clinical studies in thoracic surgery, and (iii) practical experience and published cases, to provide clinical recommendations on adverse events in NSCLC patients induced by perioperative immunotherapy.

19.
Brain Res ; 1752: 147228, 2021 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-33385377

RESUMO

The possible role of miR-194-5p in brain and neurodegenerative diseases has been reported, but its role in intracerebral hemorrhage (ICH) has not been studied. This study estimated the mechanism of miR-194-5p in ICH. ICH rat model was established by injecting collagenase type VII. miR-194-5p expression in brain tissue of ICH rats was overexpressed by injection of miR-194-5p agomir. Then neurological function score and brain water content were measured. The morphological changes of brain tissue and neuronal apoptosis were evaluated by histological staining. Levels of NLRP3 inflammasomes, IL-1ß and IL-18 were measured. The target relation between miR-194-5p and TRAF6 was verified and the binding of TRAF6 to NLRP3 was explored. miR-194-5p was decreased in ICH rats. After overexpression of miR-194-5p, the neuropathological injury in ICH rats was significantly reduced, and NLRP3-mediated inflammatory injury was inhibited. miR-194-5p targeted TRAF6. TRAF6 interacted with NLRP3 to promote the activation of NLRP3 inflammasomes. Overexpression of miR-194-5p reduced the interaction between TRAF6 and NLRP3, thereby alleviating the neuroinflammation. Collectively, overexpression of miR-194-5p reduced the TRAF6/NLRP3 interaction, thus inhibiting the activation of NLRP3 inflammasomes and reducing neuroinflammation during ICH. This study may shed new light on ICH treatment.

20.
Sci Total Environ ; 767: 144433, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33422958

RESUMO

We investigated the occurrence, compositions, and partitioning behaviors of organophosphorus flame retardants (OPFRs) in indoor dust, air, and ornamental plants in Dalian, China, to evaluate the possibility of using houseplants as indoor biosamplers of OPFRs. The mean concentrations of OPFRs in the indoor air, dust, and plant samples were 14.9 ng/m3, 18,000 ng/g, and 345 ng/g, respectively. Tris(2-chloroisopropyl) phosphate (TCIPP) was the dominant congener in all kinds of samples. Significant correlation was found between the concentrations of tris(1,3-dichloroisopropyl) phosphate (TDCIPP) in indoor air and plants, suggesting that ornamental plant can be used as a sentinel for certain OPFRs in the indoor air. We developed a predictive model to assess the partitioning coefficients of OPFRs between indoor air and plant. The lipid content in leaf cuticle instead of leaf organic matter was used to improve the accuracy and reliability of this assessment. Using this model, we can estimate the OPFR concentrations in the indoor air based on their concentrations measured in the corresponding indoor plant. The estimated air concentrations were generally comparable with the measured concentrations, especially for those with octanol-air partition coefficient log Koa <11.6. Indoor plants can also provide a more holistic understanding of OPFR occurrence within a home due to the relatively long-term air-foliage partitioning. The results suggest that under certain conditions indoor ornamental plants have the potential to be used as the biosamplers of OPFRs in the indoor environment due to their convenience and low-cost.


Assuntos
Poluição do Ar em Ambientes Fechados , Retardadores de Chama , Poluição do Ar em Ambientes Fechados/análise , China , Poeira/análise , Exposição Ambiental/análise , Monitoramento Ambiental , Retardadores de Chama/análise , Organofosfatos/análise , Compostos Organofosforados/análise , Reprodutibilidade dos Testes
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