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1.
Aging (Albany NY) ; 13(17): 21483-21496, 2021 09 12.
Artigo em Inglês | MEDLINE | ID: mdl-34511433

RESUMO

2,5-dimethyl celecoxib (DMC), a close derivative of celecoxib, has also been reported to have anticancer effects. However, the effects and underlying molecular mechanisms of DMC with respect to nasopharyngeal carcinoma are still largely unknown. In this study, we present that DMC has displayed anticancer potency in nasopharyngeal carcinoma in vitro and in vivo. Mechanistically, we found DMC induced apoptosis and autophagy for anticancer therapy against nasopharyngeal carcinoma. Furthermore, DMC-induced autophagy could remarkably attenuate after the treatment of reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC) and c-Jun N-terminal kinase (JNK) inhibitor SP600125 (SP). Taken together, these results suggested DMC induced apoptosis and autophagic death via activation of ROS/JNK axis in NPC cells, which providing us new insights into developing potential therapeutic agents for nasopharyngeal carcinoma patients.

2.
Sensors (Basel) ; 21(16)2021 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-34450809

RESUMO

The recent growth of the elderly population has led to the requirement for constant home monitoring as solitary living becomes popular. This protects older people who live alone from unwanted instances such as falling or deterioration caused by some diseases. However, although wearable devices and camera-based systems can provide relatively precise information about human motion, they invade the privacy of the elderly. One way to detect the abnormal behavior of elderly residents under the condition of maintaining privacy is to equip the resident's house with an Internet of Things system based on a non-invasive binary motion sensor array. We propose to concatenate external features (previous activity and begin time-stamp) along with extracted features with a bi-directional long short-term memory (Bi-LSTM) neural network to recognize the activities of daily living with a higher accuracy. The concatenated features are classified by a fully connected neural network (FCNN). The proposed model was evaluated on open dataset from the Center for Advanced Studies in Adaptive Systems (CASAS) at Washington State University. The experimental results show that the proposed method outperformed state-of-the-art models with a margin of more than 6.25% of the F1 score on the same dataset.


Assuntos
Atividades Cotidianas , Dispositivos Eletrônicos Vestíveis , Idoso , Humanos , Memória de Longo Prazo , Redes Neurais de Computação , Privacidade
4.
Gene ; 798: 145792, 2021 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-34175399

RESUMO

BACKGROUND: Apoptosis is a form of cell death that plays a critical role in the maintenance of tissue homeostasis involving the development and elimination of unwanted cells. Dysregulation of apoptosis appears to be associated in the pathogenesis of many human diseases. Autosomal dominant polycystic kidney disease (ADPKD) is a common monogenetic disease and is mainly caused by mutations in PKD1. Previous studies proved that increased cell death occurred in ADPKD patients and animal models. However, the role of apoptosis in kidney cystogenesis is not clear. METHODS: In current study, due to the high similarities between human and pig, PKD1-deficient (PKD1+/-) pigs and PKD1-knockdown (PKD1KD) pig kidney epithelial cells were used to investigate the mechanisms of apoptosis in driving cystogenesis. RESULTS: In PKD1+/- pigs, increased intrinsic and extrinsic apoptosis were found at ages of 1 month and 3 months, whereas the autophagy and pyroptosis were not altered. Meanwhile, the intrinsic apoptosis was activated along with untouched extrinsic apoptosis in PKD1KD pig kidney cells. Thus, the intrinsic apoptosis played important roles in cystogenesis. CONCLUSIONS: This work provides detail analysis of the roles of different cell death types during cystogenesis in ADPKD pig model. The results suggested a potential new strategy for the diagnosis and treatment of ADPKD by targeting intrinsic apoptosis.


Assuntos
Apoptose , Rim Policístico Autossômico Dominante/etiologia , Canais de Cátion TRPP/deficiência , Animais , Contagem de Células , Linhagem Celular , Modelos Animais de Doenças , Técnicas de Silenciamento de Genes , Macrófagos , Rim Policístico Autossômico Dominante/genética , Suínos , Fator de Necrose Tumoral alfa/metabolismo
5.
Sensors (Basel) ; 21(9)2021 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-33946998

RESUMO

Research on the human activity recognition could be utilized for the monitoring of elderly people living alone to reduce the cost of home care. Video sensors can be easily deployed in the different zones of houses to achieve monitoring. The goal of this study is to employ a linear-map convolutional neural network (CNN) to perform action recognition with RGB videos. To reduce the amount of the training data, the posture information is represented by skeleton data extracted from the 300 frames of one film. The two-stream method was applied to increase the accuracy of recognition by using the spatial and motion features of skeleton sequences. The relations of adjacent skeletal joints were employed to build the direct acyclic graph (DAG) matrices, source matrix, and target matrix. Two features were transferred by DAG matrices and expanded as color texture images. The linear-map CNN had a two-dimensional linear map at the beginning of each layer to adjust the number of channels. A two-dimensional CNN was used to recognize the actions. We applied the RGB videos from the action recognition datasets of the NTU RGB+D database, which was established by the Rapid-Rich Object Search Lab, to execute model training and performance evaluation. The experimental results show that the obtained precision, recall, specificity, F1-score, and accuracy were 86.9%, 86.1%, 99.9%, 86.3%, and 99.5%, respectively, in the cross-subject source, and 94.8%, 94.7%, 99.9%, 94.7%, and 99.9%, respectively, in the cross-view source. An important contribution of this work is that by using the skeleton sequences to produce the spatial and motion features and the DAG matrix to enhance the relation of adjacent skeletal joints, the computation speed was faster than the traditional schemes that utilize single frame image convolution. Therefore, this work exhibits the practical potential of real-life action recognition.


Assuntos
Algoritmos , Redes Neurais de Computação , Idoso , Bases de Dados Factuais , Atividades Humanas , Humanos , Esqueleto
6.
Sensors (Basel) ; 21(9)2021 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-33925161

RESUMO

Owing to progressive population aging, elderly people (aged 65 and above) face challenges in carrying out activities of daily living, while placement of the elderly in a care facility is expensive and mentally taxing for them. Thus, there is a need to develop their own homes into smart homes using new technologies. However, this raises concerns of privacy and data security for users since it can be handled remotely. Hence, with advancing technologies it is important to overcome this challenge using privacy-preserving and non-intrusive models. For this review, 235 articles were scanned from databases, out of which 31 articles pertaining to in-home technologies that assist the elderly in living independently were shortlisted for inclusion. They described the adoption of various methodologies like different sensor-based mechanisms, wearables, camera-based techniques, robots, and machine learning strategies to provide a safe and comfortable environment to the elderly. Recent innovations have rendered these technologies more unobtrusive and privacy-preserving with increasing use of environmental sensors and less use of cameras and other devices that may compromise the privacy of individuals. There is a need to develop a comprehensive system for smart homes which ensures patient safety, privacy, and data security; in addition, robots should be integrated with the existing sensor-based platforms to assist in carrying out daily activities and therapies as required.


Assuntos
Atividades Cotidianas , Privacidade , Idoso , Envelhecimento , Segurança Computacional , Humanos , Tecnologia
7.
J Cell Mol Med ; 25(7): 3548-3559, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33728802

RESUMO

Growth arrest-specific 5 (GAS5) is a kind of long non-coding RNAs (lncRNAs). Previous studies showed that down-regulation of LncRNA-GAS5 was involved in the development of systemic lupus erythematosus (SLE). However, the regulatory mechanism of down-expressed LncRNA-GAS5 in SLE remains obscure. In this study, we aimed to investigate the association of LncRNA-GAS5 polymorphism with SLE risk. And further explore how LncRNA-GAS5 is involved in the occurrence of SLE. Here, we evaluated the relationship between the risk for the development of SLE and the 5-base pair (AGGCA/-) insertion/deletion (I/D) polymorphism (rs145204276) in the LncRNA-GAS5 promoter region. A custom 36-Plex SNPscan kit was used for genotyping the LncRNA-GAS5 polymorphisms. The LncRNA-GAS5 and miR-21 target prediction was performed using bioinformatics software. Enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR (qRT-PCR) were performed to assess GAS5 and miR-21 mRNA expression and PTEN protein expression. The results revealed that rs145204276 resulted in a decreased risk of SLE (DD genotypes vs II genotypes: adjusted OR = 0.538, 95% CI, 0.30-0.97, P = .039; ID genotypes vs II genotypes: adjusted OR = 0.641, 95% CI, 0.46-0.89, P = .007; ID/DD genotypes vs II genotypes: adjusted OR = 0.621, 95% CI, 0.46-0.84, P = .002; D alleles vs I alleles: adjusted OR = 0.680, 95% CI, 0.53-0.87, P = .002). A reduced incidence of renal disorders in SLE was found to be related to ID/DD genotypes and D alleles (ID/DD genotypes vs II genotypes: OR = 0.57, 95% CI, 0.36-0.92, P = .020; D alleles vs I alleles: OR = 0.63, 95% CI, 0.43-0.93, P = .019). However, no significant association of rs2235095, rs6790, rs2067079 and rs1951625 polymorphisms with SLE risk was observed (P > .05). Additionally, haplotype analysis showed that a decreased SLE risk resulted from the A-A-C-G-D haplotype (OR = 0.67, 95% CI, 0.49-0.91, P = .010). Also, patients in the SLE group showed a down-regulated expression of LncRNA-GAS5 and PTEN than the healthy volunteers; however, patients with rs145204276 ID/DD genotypes showed up-regulated expression of LncRNA-GAS5 and PTEN compared with patients carrying the II genotype. Furthermore, the miR-21 levels were considerably up-regulated in the SLE group than the healthy volunteers, and patients with rs145204276 ID/DD genotype had lower miR-21 levels than the ones with the II genotype. Thus, we found that the LncRNA-GAS5/miR-21/PTEN signalling pathway was involved in the development of SLE, where LncRNA-GAS5 acted as an miR-21 target, and miR-21 regulated the expression of PTEN. These findings indicated that the rs145204276 ID/DD genotypes in the LncRNA-GAS5 gene promoter region may be protected against SLE by up-regulating the expression of LncRNA-GAS5, which consecutively regulated miR-21 and PTEN levels.


Assuntos
Lúpus Eritematoso Sistêmico/genética , Lúpus Eritematoso Sistêmico/metabolismo , MicroRNAs/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Polimorfismo de Nucleotídeo Único , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Adulto , Grupo com Ancestrais do Continente Asiático , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Leucócitos Mononucleares/metabolismo , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Regiões Promotoras Genéticas , Transdução de Sinais
9.
J Cell Physiol ; 235(11): 8768-8778, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32633026

RESUMO

Mutation in the tumor suppressor gene p53 is the most frequent molecular defect in endometrial carcinoma (EC). Recently, CP-31398, a p53-stabilizing compound, has been indicated to possess the ability to alter the expression of non-p53 target genes in addition to p53 downstream genes in tumor cells. Herein, we explore the alternative mechanisms underlying the restoration of EC tumor suppressor function in mutant p53 by CP-31398. A p53-mutated EC cell was constructed in AN3CA cells with restored or partial loss of Slug using lentiviral vectors, followed by treatment with 25 µM CP-31398. A p53-independent mechanism of CP-31398 was confirmed by the interaction between mouse double minute 2 homolog (MDM2) and Slug AN3CA cells treated with IWR-1 (inhibitor of Wnt response 1). Furthermore, the AN3CA cells were treated with short hairpin RNA against Slug, Wnt-specific activators (LiCl) or inhibitors (XAV-939) followed by CP-31398 treatment. Moreover, AN3CA cell proliferation and apoptosis were examined. A tumorigenicity assay was conducted in nude mice. CP-31398 could promote the apoptosis of p53-mutated EC cells, while Slug reversed this effect. Slug ubiquitination was found to occur via binding of Slug to MDM2 in AN3CA cells. We found that CP-31398 increased the GSK-3ß, p-Slug, Puma, Wtp53, and Bax expressions whereas Wnt, Mtp-53, Slug, Bcl-2, and Ki-67 expressions were decreased. However, these findings were reversed following the activation of the Wnt pathway and overexpression of Slug. Finally, the in vivo experimental evidence confirmed that CP-31398 with depleted Slug suppressed tumor growth by downregulating the Slug. Collectively, CP-31398-regulated Slug downregulation represses the p53-mutated EC via the p53/Wnt/Puma pathway.


Assuntos
Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Neoplasias do Endométrio/tratamento farmacológico , Pirimidinas/farmacologia , Proteína Supressora de Tumor p53/metabolismo , Animais , Linhagem Celular Tumoral , Neoplasias do Endométrio/metabolismo , Feminino , Humanos , Camundongos Nus , Proteínas Proto-Oncogênicas c-mdm2/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteína Supressora de Tumor p53/genética
10.
J Nanosci Nanotechnol ; 20(12): 7516-7521, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-32711621

RESUMO

UV light driven photoelectric properties of ZnO film to humidity were researched. ZnO film was prepared through the method of screen printing sustained on Al2O3 substrate. ZnO was characterized by XRD, FE-SEM and EDX. The time-dependent UV light driven photoelectric properties of ZnO were investigated by exposing it to different bias voltages and different relative humidity (20% RH, 40% RH, 60% RH and 80% RH). On one hand, the photoelectric properties of ZnO increased with the augmenting of bias voltage, which shows that a higher bias causes more separation of carriers. On the other hand, the photocurrent decreased with the increase in relative humidity, which shows that bigger humidity results in smaller photoelectric property. To discuss these results, corresponding possible illustrations for the photoelectric properties under different conditions were proposed.

11.
Surg Infect (Larchmt) ; 21(9): 760-765, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32716758

RESUMO

Background: In the ongoing coronavirus disease 2019 (COVID-19) pandemic, resuming provision of surgical services poses a challenge given that patients may have acute surgical pathologies with concurrent COVID-19 infection. We utilized a risk-stratified approach to allow for early recognition and isolation of potential COVID-19 infection in surgical patients, ensuring continuity of surgical services during a COVID-19 outbreak. Patients and Methods: Over a four-month period from January to April 2020, surgical patients admitted with concurrent respiratory symptom, infiltrates on chest imaging, or suspicious travel/epidemiologic history were placed in a dedicated ward in which they were tested for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). If emergency operations were necessary prior to the exclusion of COVID-19, patients were managed as per suspected cases of COVID-19, with appropriate precautions and full personal protective equipment (PPE). Results: From January through April 2020, a total of 8,437 patients were admitted to our surgical department; 5.9% (498/8437) required peri-operative testing for SARS-CoV-2. Because testing was in-house with turnaround within 24 hours, only a small number of emergency operations (n = 10) were conducted for suspected COVID-19 cases prior to results; none tested positive. The testing yield was lower in surgical inpatients compared with medical inpatients (odds ratio [OR] = 0.20, 95% confidence interval [CI], 0.12-0.32, p < 0.001). Three operations were conducted in known COVID-19 cases; all healthcare workers (HCWs) used full PPE. A risk-stratified testing strategy picked up previously unsuspected COVID-19 in six cases; 66.7% (4/6) were asymptomatic at presentation. Although 48 HCWs were exposed to these six cases, delayed diagnosis was averted and no evidence of spread to patients or HCWs was detected. Conclusion: A risk-stratified approach allowed for early recognition, testing, and isolation of potential COVID-19 infection in surgical patients, ensuring continuity of surgical services.


Assuntos
Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/fisiopatologia , Pacientes Internados , Isolamento de Pacientes/métodos , Pneumonia Viral/diagnóstico , Pneumonia Viral/fisiopatologia , Adulto , COVID-19 , Surtos de Doenças , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Equipamento de Proteção Individual , Medição de Risco , Singapura , Procedimentos Cirúrgicos Operatórios , Centros de Atenção Terciária
12.
Cancer Manag Res ; 12: 4595-4604, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606952

RESUMO

Cancer is the leading cause of death, placing a substantial global health burden. The development of the most effective treatment regimen is the unmet clinical need for cancer. Inflammation plays a role in tumorigenesis and progression, and anti-inflammation may be a promising option for cancer management and prevention. Emerging studies have shown that non-steroidal anti-inflammatory drugs (NSAIDs) display anticarcinogenic and chemopreventive properties through the regulation of autophagy in certain types of cancer. In this review, we summarize the pharmacological functions and side effects of NSAIDs as chemotherapeutic agents, and focus on its mode of action on autophagy regulation, which increases our knowledge of NSAIDs and cancer-related inflammation, and contributes to a putative addition of NSAIDs in the chemoprevention and treatment of cancer.

13.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(3): 894-898, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32552954

RESUMO

OBJECTIVE: To investigate the influence of conventional CAG regimen and decitabine + decreased dose CAG (D+dCAG) regimen on the clinical efficacy and safety of patients with MDS-RAEB/AML-MRC. METHODS: The clinical data of 67 patients with MDS-RAEB/AML-MRC hospitalized in our hospital from March 2012 to July 2017 were analyzed retrospectively. According to chemotherapecctic regimens, 76 patients were divided into 2 groups: 37 patients treated with conventional CAG regimen were enrolled in control group, 30 patients treated with decitabine + decreased dose CAG regimen were enrolled in D+dCAG group. The complete remission (CR) rate, overall remission rate (ORR), OS and PFS time and incidence of adverse reactions in 2 groups were compared. RESULTS: The CR in D+dCAG group was significantly higher than that in control group (P<0.05). ORR was not significanly different between 2 groups (P>0.05). There was no significant difference in the cumulative OS rate between 2 groups (P>0.05). There was no significant difference in the cumulative OS rate and PFS rate in nonimplantation between 2 groups (P>0.05). The incidence of adverse reactions of hematological system, pulmonary infection, skin and soft tissue infection, agranulocytosic fever and mycotic infection was not significanly different between 2 groups (P>0.05). The duration of granulocyte deficiency and platelet count less than 20×109/L were not significanly different between 2 groups (P>0.05). CONCLUSION: Compared with conventional CAG regimen, decitabine + decreased dose CAG regimen in the treatment of patients with MDS-RAEB/AML-MRC can efficiently improve the remission effects and showed the well overall safety, but can not increase the survival rate.


Assuntos
Anemia Refratária com Excesso de Blastos , Leucemia Mieloide Aguda , Síndromes Mielodisplásicas , Protocolos de Quimioterapia Combinada Antineoplásica , Citarabina , Decitabina , Fator Estimulador de Colônias de Granulócitos , Humanos , Estudos Retrospectivos , Resultado do Tratamento
14.
Antib Ther ; 3(2): 63-70, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32391516

RESUMO

Dickkopf-related protein 2 (DKK2)is a member of the Dickkopf family in Wnt signaling pathway. Recently, we found that antibodies against DKK2 could activate natural killer (NK) and CD8+ T cells in tumors and inhibit tumor growth. In this paper, we report the rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal anti-DKK2 antibodies. To break the immune tolerance, we designed and chemically synthesized six peptides corresponding to different regions of DKK2 as immunogens and found five of them could generate mouse polyclonal antibodies that can bind to the active recombinant human DKK2 protein. Neutralizing mouse monoclonal antibodies (5F8 and 1A10) against human DKK2 were successfully developed by immunizing the mice with two different peptides (34KLNSIKSSL42 and 240KVWKDATYS248) conjugated to Keyhole limpet hemocyanin (KLH). The monoclonal antibodies not only abolish DKK2's suppression of Wnt signaling in vitro but also inhibits tumor growth in vivo. Currently, those two mAbs are undergoing humanization as immunotherapy candidates and may offer a new drug for treatment of human cancers.

15.
Chest ; 158(1): e9-e13, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32243945

RESUMO

As of March 24, 2020, novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for 379,661 infection cases with 16,428 deaths globally, and the number is still increasing rapidly. Herein, we present four critically ill patients with SARS-CoV-2 infection who received supportive care and convalescent plasma. Although all four patients (including a pregnant woman) recovered from SARS-CoV-2 infection eventually, randomized trials are needed to eliminate the effect of other treatments and investigate the safety and efficacy of convalescent plasma therapy.


Assuntos
Antivirais , Infecções por Coronavirus , Estado Terminal/terapia , Pandemias , Pneumonia Viral , Complicações Infecciosas na Gravidez , Adulto , Idoso , Antifúngicos/administração & dosagem , Antivirais/administração & dosagem , Antivirais/classificação , Betacoronavirus/isolamento & purificação , COVID-19 , Teste para COVID-19 , Técnicas de Laboratório Clínico/métodos , Infecções por Coronavirus/complicações , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/fisiopatologia , Infecções por Coronavirus/terapia , Oxigenação por Membrana Extracorpórea/métodos , Feminino , Humanos , Imunização Passiva/métodos , Masculino , Pessoa de Meia-Idade , Pneumonia Viral/complicações , Pneumonia Viral/diagnóstico , Pneumonia Viral/microbiologia , Pneumonia Viral/fisiopatologia , Pneumonia Viral/terapia , Gravidez , Complicações Infecciosas na Gravidez/fisiopatologia , Complicações Infecciosas na Gravidez/terapia , Complicações Infecciosas na Gravidez/virologia , Radiografia Torácica/métodos , Respiração Artificial/métodos , SARS-CoV-2 , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento
16.
Biosci Rep ; 40(5)2020 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-32319512

RESUMO

Precartilaginous stem cells (PCSCs) are adult stem cells that can initiate chondrocytes and bone development. In the present study, we explored whether miR-132/212 was involved in the proliferation of PCSCs via Hedgehog signaling pathway. PCSCs were isolated and purified with the fibroblast growth factor receptor-3 (FGFR-3) antibody. Cell viability, DNA synthesis and apoptosis were measured using MTT, BrdU and flow cytometric analysis. The mRNA and protein expression were detected by real-time PCR and Western blot, respectively. The target gene for miR-132/212 was validated by luciferase reporter assay. Results showed that transfection with miR-132/212 mimic significantly increased cell viability and DNA synthesis, and inhibited apoptosis of PCSCs. By contrast, miR-132/212 inhibitor could suppress growth and promote apoptosis of PCSCs. Luciferase reporter assays indicated that transfection of miR-132/212 led to a marked reduction of luciferase activity, but had no effect on PTCH1 3'-UTR mutated fragment, suggesting that Patched1 (PTCH1) is a target of miR-132/212. Furthermore, treatment with miR-132/212 mimics obviously increased the protein expression of Indian hedgehog (Ihh) and parathyroid hormone related protein (PTHrP), which was decreased after treatment with Hedgehog signaling inhibitor, cyclopamine. We also found that inhibition of Ihh/PTHrP signaling by cyclopamine significantly suppressed growth and DNA synthesis, and induced apoptosis in PCSCs. These findings demonstrate that miR-132/212 promotes growth and inhibits apoptosis in PCSCs by regulating PTCH1-mediated Ihh/PTHrP pathway, suggesting that miR-132/212 cluster might serve as a novel target for bone diseases.


Assuntos
Células-Tronco Adultas/fisiologia , Proliferação de Células/genética , Condrócitos/fisiologia , MicroRNAs/metabolismo , Animais , Apoptose/efeitos dos fármacos , Cartilagem Articular/citologia , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Proteínas Hedgehog/antagonistas & inibidores , Proteínas Hedgehog/metabolismo , Família Multigênica , Proteína Relacionada ao Hormônio Paratireóideo/metabolismo , Receptor Patched-1/metabolismo , Cultura Primária de Células , Coelhos , Alcaloides de Veratrum/farmacologia
17.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 93-97, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027259

RESUMO

OBJECTIVE: To analyze the correlation of the minimal residual disease level with the prognosis of the AML patients with NPM1 gene mutation positive after chemotherapy. METHODS: The clinical data of 112 newly diagnosed adult AML patients with positive NPM1 gene were collected and retrospectively analyzed. The correlation of the transcripts of NPM1 gene mutation with prognosis of patients was analyzed. RESULTS: In 112 AML patients, the median transcript level of NPM1 gene mutation accounted for 83.68% (5.86%-486.57%), FLT3-ITD mutation positive was found in 44 cases (39.29%), chromosomal abnormalities in 22 cases (19.64%) and complete remission in 96 cases (85.71%). Multivariate logistic regression analysis showed that the initial induction therapy and white blood cell count closely related with complete remission (P<0.05). The median follow-up time was 22 (3-36) months, and the 3-year overall survival rate was 66.07% in 112 patients. Multivariate logistic regression analysis showed that both the high level of minimal residual disease at the initial complete remission and the high level of minimal residual disease after consolidation therapy were the independent risk factors for overall survival (P<0.05). CONCLUSION: In newly diagnosed adult AML patients with NPM1 mutation positive, the early high level of minimal residual disease after chemotherapy closely relates with poor prognosis.


Assuntos
Leucemia Mieloide Aguda , Proteínas Nucleares/genética , Adulto , Humanos , Leucemia Mieloide Aguda/genética , Mutação , Neoplasia Residual , Prognóstico , Estudos Retrospectivos , Tirosina Quinase 3 Semelhante a fms
18.
Sensors (Basel) ; 19(23)2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31816832

RESUMO

We propose a portable and wireless acquisition system to help consumers or users register important physiological signals. The acquisition system mainly consists of a portable device, a graphic user interface (GUI), and an application program for displaying the signals on a notebook (NB) computer or a smart device. Essential characteristics of the portable device include eight measuring channels, a powerful microcontroller unit, a lithium battery, Bluetooth 3.0 data transmission, and a built-in 2 GB flash memory. In addition, the signals that are measured can be displayed on a tablet, a smart phone, or a notebook computer concurrently. Additionally, the proposed system provides extra power supply sources of ±3 V for the usage of external circuits. On the other hand, consumers or users can design their own sensing circuits and combine them with this system to carry out ubiquitous physiological studies. Four major advantages in the proposed system are the capability of combining it with a NB computer or a smart phone to display the signals being measured in real time, the superior mobility due to its own independent power system, flash memory, and good expandability. Briefly, this acquisition system offers consumers or users a convenient and portable studying tool to measure dynamic vital signals of interest in psychological and physiological research fields.


Assuntos
Monitorização Ambulatorial/instrumentação , Processamento de Sinais Assistido por Computador , Tecnologia sem Fio , Gráficos por Computador , Computadores , Computadores de Mão , Desenho de Equipamento , Humanos , Monitorização Ambulatorial/métodos , Smartphone , Telemetria/instrumentação , Interface Usuário-Computador
19.
Sensors (Basel) ; 19(19)2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31569666

RESUMO

In this paper, we propose an intelligent lecturer tracking and capturing (ILTC) system to automatically record course videos. Real-time and stable lecturer localization is realized by combining face detection with infrared (IR) thermal sensors, preventing detection failure caused by abrupt and rapid movements in face detection and solving the non-real-time sensing problem for IR thermal sensors. Further, the camera is panned automatically by a servo motor controlled with a microcontroller to keep the lecturer in the center of the screen. Experiments were conducted in a classroom and a laboratory. Experimental results demonstrated that the accuracy of the proposed system is much higher than that of the system without IR thermal sensors. The survey of 32 teachers from two universities showed that the proposed system is a more practical utility and meets the demand of increasing online courses.

20.
Int J Biol Sci ; 15(9): 1993-2005, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31523199

RESUMO

Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) is a severe infectious disease in the swine industry. PRRSV infection is mediated by porcine CD163 (pCD163). Scavenger receptor cysteine-rich domain 5 coded by exon 7 of pCD163 is essential for PRRSV infection. In this study, we generated CD163 exon 7 deleted (CD163E7D) pigs using CRISPR/Cas9 mediated homologous recombination and somatic cell nuclear transfer (SCNT). The deletion of exon 7 had no adverse effects on CD163-associated functions. Pigs were further challenged with a highly pathogenic PRRSV (HP-PRRSV) strain. The CD163E7D pigs exhibited mild clinical symptoms and had decreased viral loads in blood. All CD163E7D pigs survived the viral challenge, while all the WT pigs displayed severe symptoms, and 2 out of 6 WT pigs died during the challenge. Our results demonstrated that CD163 exon 7 deletion confers resistance to HP-PRRSV infection without impairing the biological functions of CD163.


Assuntos
Antígenos CD/genética , Antígenos de Diferenciação Mielomonocítica/genética , Éxons/genética , Síndrome Respiratória e Reprodutiva Suína/genética , Síndrome Respiratória e Reprodutiva Suína/prevenção & controle , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Receptores de Superfície Celular/genética , Animais , Sistemas CRISPR-Cas/genética , Técnicas de Transferência Nuclear , Suínos
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