Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 71
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Mater Chem B ; 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35521746

RESUMO

Chronic wounds, especially diabetic wounds, involve abnormally long inflammatory periods due to their pathological microenvironment of high reactive oxygen species (ROS) levels and lack of blood vessels. Here, via a mild, simple and feasible fabrication approach, a sustained oxygenation system is proposed, consisting of MnO2 nanosheets and a dual-network hydrogel fabricated from natural biomaterials including silk fibroin (SF) and carboxymethyl cellulose (CMC). Compared with the initial value (61.09 kPa), the compression modulus of the dual-network hydrogel increased by 116.2% through the coordination of strong covalent bonds and sacrificial coordination bonds constructed by enzymatic crosslinking and UV-irradiation crosslinking; the intrinsic shear-thinning effect endows the dual-network hydrogel with satisfactory injectable properties to be customized as a predetermined shape to accommodate the irregular wounds of diabetes. The encapsulated MnO2 nanosheets can catalyze the excessive ROS into necessary O2in situ and, after co-incubating with the SF/CMC@MnO2 hydrogels, cells in oxidative stress show significantly lower ROS (3 times) and higher O2 (17 times) levels that are conductive to relieving oxidative stress, promoting angiogenesis and reducing inflammation in vivo. Meanwhile, these SF-based hydrogels can offset the overexpression of matrix metalloproteinases (MMPs) in diabetic wounds (more than 80%) and promote remodeling of the extracellular matrix. Eventually, wound healing rates >76% in 7 days and 100% in 14 days were achieved by the bio-fabricated nanocomposite hydrogel and are remarkably faster than the commercial dressing healing rates (<30% in 7 days and <80% in 14 days). These results indicate that this bio-fabricated hydrogel system with multiple and customizable functions has great promise in the personalized clinical care of chronic wounds.

2.
Front Med (Lausanne) ; 9: 872881, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572990

RESUMO

Background and Aims: Emergency endoscopy is recommended for patients with acute esophageal variceal bleeding (EVB) and their prognosis has improved markedly over past decades due to the increased specialization of endoscopic practice. The study aimed to compare outcomes following emergency endoscopic injection sclerotherapy (EIS) and endoscopic variceal ligation (EVL) in cirrhotic patients with acute EVB. Methods: Cirrhotic patients with acute EVB who underwent emergency endoscopy were retrospectively enrolled from 2013 to 2020 across 34 university hospitals from 30 cities. The primary outcome was the incidence of 5-day rebleeding after emergency endoscopy. Subgroup analysis was stratified by Child-Pugh class and bleeding history. A 1:1 propensity score matching (PSM) analysis was performed. Results: A total of 1,017 and 382 patients were included in EIS group and EVL group, respectively. The 5-day rebleeding incidence was similar between EIS group and EVL group (4% vs. 5%, P = 0.45). The result remained the same after PSM (P = 1.00). Among Child-Pugh class A, B and C patients, there were no differences in the 5-day rebleeding incidence between the two groups after PSM (P = 0.25, 0.82, and 0.21, respectively). As for the patients with or without bleeding history, the differences between EIS group and EVL group were not significant after PSM (P = 1.00 and 0.26, respectively). Conclusion: The nationwide cohort study indicates that EIS and EVL are both efficient emergency endoscopic treatment strategies for acute EVB. EIS should not be dismissed as an economical and effective emergency endoscopic treatment strategy of acute EVB. ClincialTrials.gov number NCT04307264.

3.
Biomater Sci ; 10(9): 2417-2427, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35393995

RESUMO

Adhesives for gastric hemorrhage are of great clinical significance. However, it remains a major challenge in clinics due to its poor stability under acidic environments and low adhesion to wet tissues. Herein, inspired by the high adhesiveness of the ascidian secretory protein, we designed a series of aciduric bionic hydrogel adhesives (PDTAs) based on poly(γ-glutamic acid) (γ-PGA) and tannic acid (TA). The formation of hydrogel adhesives was attributed to the abundant hydrogen bonds between amide groups of PGA-DA and polyphenol groups of TA. These hydrogel adhesives exhibited enhanced wet tissue adhesion (400%), higher stretchability (800% elongation), and aciduric stability (7 days) compared with commercial fibrin glue. Rodent wound models indicated that the hydrogel adhesives demonstrated significant healing promotion due to ameliorating collagen deposition and angiogenesis. These hydrogel adhesives show great potential in treating gastric hemorrhages and promoting wound healing.


Assuntos
Hidrogéis , Urocordados , Adesividade , Adesivos/farmacologia , Animais , Hemostasia , Hidrogéis/química , Taninos/química , Taninos/farmacologia , Cicatrização
4.
J Ethnopharmacol ; 292: 115216, 2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-35331875

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Composed of dried Aconitum pendulum and Aconitum flavum roots, Tiebangchui, is an important Tibetan medicine and has been traditionally and widely used as a remedy for cold and pain for thousands of years because of its extraordinary pharmacological activities. The toxicity and efficacy of Tiebangchui as a typical toxic traditional Tibetan medicine, are interdependent, and thus to make sure its safe use in clinics is also noteworthy. AIM OF THE STUDY: This review aims to document and summarize critical and comprehensive information about traditional uses, phytochemistry, pharmacology, toxicology and processing methods of Tiebangchui. Perspectives for possible future investigations have been discussed. MATERIALS AND METHODS: Relevant information about Tiebangchui (A. pendulum and A. flavum) was collected from internationally recognized electronic scientific databases, such as Web of Science, PubMed, Science Direct, Springer Link, ACS, and CNKI. Then, classic Tibetan medical books, such as Four Medical Tantra, and Jing Zhu Materia Medica, and official drug standards were reviewed. RESULTS: A total of 95 chemical constituents have been isolated and identified from Tiebangchui, and most of them were diterpenoid alkaloids. These phytochemicals showed a wide range of pharmacological properties, such as anti-inflammation, anti-rheumatoid arthritis, analgesic, local anesthetic, anti-cancer and anti-bacterial activities. Hence, Tiebangchui is broadly used in hundreds of preparations to treat fever, arthritis, rheumatic arthralgia, traumatic injury, furuncle and swelling. Cardiotoxicity, neurotoxicity and gastrointestinal toxicity are the main toxic effects caused by the Aconitum alkaloids of Tiebangchui. Various processing methods, including steaming, decocting and sand-frying, and traditional Tibetan medicine processing methods, such as processing with Hezi decoction, Qingke wine and Zanba, are effective in attenuating toxicity while retaining efficacy. CONCLUSIONS: The present review provides primary information of Tiebangchui, particularly for its traditional uses, botanical characteristics, phytochemicals, outstanding bioactivities and processing methods. However, studies that explored the in vivo pharmacokinetics and mechanism of Tiebangchui, as well as its quality markers, qualitative and quantitative analysis are still insufficient. Processing methods that attenuate toxicities, evaluations of efficacy, in vivo processes and biological effects, the mechanisms of processed products should be further explored.


Assuntos
Aconitum , Alcaloides , Medicamentos de Ervas Chinesas , Aconitum/química , Alcaloides/análise , Medicamentos de Ervas Chinesas/farmacologia , Etnofarmacologia/métodos , Compostos Fitoquímicos/uso terapêutico , Compostos Fitoquímicos/toxicidade , Extratos Vegetais/farmacologia , Raízes de Plantas/química
5.
Histol Histopathol ; : 18446, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-35229281

RESUMO

Asiatic acid can attenuate osteoporosis through suppressing adipogenic differentiation and osteoclastic differentiation. Oxidative stress enhances osteoclastic differentiation but represses osteogenic differentiation to promote osteoporosis. However, the role and mechanism of asiatic acid in osteoporosis have not been reported. Firstly, mice were fed with high-fat-diet (HFD) with or without asiatic acid for 16 weeks. Data from an automatic biochemical analyzer showed that HFD induced down-regulation of high-density lipoprotein (HDL) and an increase of serum levels of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein (LDL). However, asiatic acid administration attenuated the decrease of HDL and increase of serum TG, TC and LDL in osteoporotic mice. Secondly, HFD induced high levels of malondialdehyde (MDA) and reactive oxygen species (ROS), low levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in osteoporotic mice. However, the levels of MDA, ROS, SOD and GSH-Px in osteoporotic mice were reversed by asiatic acid administration. (this section is unclear and requires revision) Asiatic acid administration reduced expression of c-telopeptide of type 1 collagen (CTX-1), enhanced alkaline phosphatase (ALP) and procollagen type 1 N-terminal propeptide (P1NP) in HFD-induced osteoporotic mice. (this section is unclear and requires revision) Thirdly, asiatic acid promoted calcium deposition in bone marrow cells and osteogenic differentiation in osteoporotic mice, but decreased ALP in bone marrow cells. Lastly, asiatic acid enhanced SIRT1 and nuclear FOXO1 (Nu-FOXO1) expression, while it reduced Acetyl FOXO1 (Ac-FOXO1) in osteoporotic mice. In conclusion, asiatic acid might inhibit oxidative stress and promote osteogenic differentiation through activating SIRT1/FOXO1 to attenuate HFD-induced osteoporosis in mice.

6.
Int J Phytoremediation ; : 1-9, 2022 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-35040734

RESUMO

Green plants can effectively retain atmospheric particle pollution and purify the air. As an important part of the campus ecosystem, they have a significant effect on absorbing atmospheric pollutants and improving the quality of the campus air environment. Six common greening plants were selected as the object in Xiangnan University. The dust retention capacity of plant leaves, leaf micro-structure, and leaf contact angle were measured. The results show that the highest amounts of dust removed by Osmanthus fragrans Lour was 3.451 g/m2, and the least amounts of dust removed by Magnolia denudata Desr. was 1.005 g/m2, and the maximum is 4.148 times of the minimum. The micro-structure of the plant leaf surface affects the amount of dust retention. The plant dust retention capacity with gully of the leaf surface and fluff, stomata, and sawtooth structure is relatively strong; The plant dust retention capacity with smooth, wax layer, leathery, and gully is relatively weak. The leaf contact area of a plant is related to its dust retention ability, that is, when the measured leaf contact angle is <90°, the leaf dust retention ability of the plant is strong. When the blade contact angle is >90°, the dust retention capacity is weak.


Novelty StatementMany studies have found that green plants can effectively retain atmospheric particle pollution and purify the air. As an important part of the campus ecosystem, few people specially study the campus ecosystem. Many scholars focus on the research on urban street trees and different districts of the city. The object of this study is more in-depth and targeted. Through the analysis of the difference of dust retention ability between common plants in campus, this paper compares and analyzes the influencing factors of the difference of dust retention ability among plants, which is not only related to the external environment but also closely related to the micro-structure characteristics of plant leaves. The research on the dust retention ability of different plants will help to better control dust and atmospheric environmental pollution, improve air quality, and select plants with strong dust retention ability as green plants on campus, which will play an important role in reducing dust pollution on campus.

7.
Sci China Mater ; 65(1): 246-254, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34413988

RESUMO

Although conventional suturing techniques are commonly used in assisting wound closure, they do pose limited conduciveness and may lead to secondary injury to wound tissues. Inspired by marine organism mussels, we designed and manufactured a bio-inspired hydrogel-based bandage with tough wet tissue adhesion to substitute traditional surgical suture, accelerate wound healing and avoid infection. Poly(γ-glutamic acid) was modified with 3,4-dihydroxyphenylalanine and glycidyl methacylate, then introduced into the acrylic acid-co-acrylamide hydrogel matrix with robust mechanical properties. The hydrogel bandage showed strong chemical linkage adhesion (70 ± 2.1 kPa), which is 2.8 times that of commercial tissue adhesive fibrin glue (25 ± 2.2 kPa). The hydrogel bandage can not only maintain the self-stability, but is also capable of self-tuning adhesive strength in the range of 14-70 kPa to achieve different adhesion effects by tuning constituent ratio. The bandage has desirable compression properties (0.7 ± 0.11 MPa) and tensile elongation (about 25 times), which ensures its resistance to damages, especially in joint spaces. Secondly, the bandage was endowed with antioxidant and endogenous broad-spectrum antibacterial properties with its catechol structure. Results also demonstrated excellent cell compatibility and blood compatibility, certifying its eligible biological safety profile. In a rat full-thickness cutaneous deficiency model, we can clearly observe that the bandage possesses the ability to promote wound healing (only need 6 days). Above all, this research provides a new strategy for the emergency treatment of liver hemostasis and myocardial repair during disaster rescue. SUPPLEMENTARY INFORMATION: Experimental details and supporting data are available in the online version of the paper10.1007/s40843-021-1724-8.

8.
Carbohydr Polym ; 275: 118692, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34742419

RESUMO

Traditional hydrogels often fail to match the dynamic interactions between mechanical and cellular behaviors exhibited by the natural cartilage extracellular matrix. In this research, we constructed a novel hybrid hydrogels system based on sodium alginate and polyglutamic acid. By controlling the grafting rate and concentration of polymer, the gelation time and mechanical strength can be adjusted between range of 8-28 s and 60-144 kPa. By adding microcrystalline cellulose into the system, so that the degradation time was prolonged (125%) and the swelling rate was reduced (470%). Additionally, the presence of hydrazone bonds gives the system some dynamic response characteristics, and the hydrogel exhibits excellent self healing and injectable ability. It was found that the system had positive cytocompatibility (80%), which accelerated regulatory gene expression in cartilage tissue. In conclusion, this injectable hydrogel with self-healing and customizable mechanical strength will have broad application prospects in future biomedical engineering.


Assuntos
Alginatos/química , Diferenciação Celular/efeitos dos fármacos , Condrogênese/efeitos dos fármacos , Hidrogéis/química , Células-Tronco Mesenquimais/metabolismo , Ácido Poliglutâmico/análogos & derivados , Alginatos/farmacologia , Cartilagem/metabolismo , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Celulose/química , Matriz Extracelular/metabolismo , Humanos , Ácido Poliglutâmico/química , Ácido Poliglutâmico/farmacologia , Polímeros/química , Engenharia Tecidual/métodos , Tecidos Suporte/química
9.
Immun Ageing ; 18(1): 45, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34879879

RESUMO

BACKGROUND: Studies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. The object of this study was to explore the role and mechanism of microRNA miR-425-5p in the differentiation of MSC. METHODS: The expression of miR-425-5p in MSC was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell proliferation, cell cycle and apoptosis were detected by CCK-8 colorimetry and flow cytometry. The expression of TNF were detected by ELISA. RESULTS: Our data show that MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation. ANXA2 is also the target of miR-425-5p and ANXA2 was involved in TNF-induced MSC cell apoptosis, proliferation, and differentiation. In addition, MiR-425-5p enhanced osteoporosis in mice. CONCLUSION: MiR-425-5p might serve as a potential therapeutic target for the treatment of osteoporosis.

10.
Inorg Chem ; 60(22): 17201-17211, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34735136

RESUMO

A polycrystalline iridate Li8IrO6 material was prepared via heating Li2O and IrO2 starting materials in a sealed quartz tube at 650 °C for 48 h. The structure was determined from Rietveld refinement of room-temperature powder neutron diffraction data. Li8IrO6 adopts the nonpolar space group R3̅ with Li atoms occupying the tetrahedral and octahedral sites, which is supported by the electron diffraction and solid-state 7Li NMR. This results in a crystal structure consisting of LiO4 tetrahedral layers alternating with mixed IrO6 and LiO6 octahedral layers along the crystallographic c-axis. The +4 oxidation state of Ir4+ was confirmed by near-edge X-ray absorption spectroscopy. An in situ synchrotron X-ray diffraction study of Li8IrO6 indicates that the sample is stable up to 1000 °C and exhibits no structural transitions. Magnetic measurements suggest long-range antiferromagnetic ordering with a Néel temperature (TN) of 4 K, which is corroborated by heat capacity measurements. The localized effective moment µeff (Ir) = 1.73 µB and insulating character indicate that Li8IrO6 is a correlated insulator. First-principles calculations support the nonpolar crystal structure and reveal the insulating behavior both in paramagnetic and antiferromagnetic states.

11.
Carbohydr Polym ; 272: 118489, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34420745

RESUMO

Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein. This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties. It is found that storage proteins and starch biosynthetic enzymes influence each other. The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content. In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000). This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.


Assuntos
Amido , Amilopectina , Amilose , Cicer/química , Oryza/química , Amido/química
12.
Carbohydr Polym ; 270: 118330, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34364592

RESUMO

Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials. The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior. Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa). Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments. Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one. Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.


Assuntos
Cartilagem/citologia , Sulfatos de Condroitina/química , Hidrogéis/química , Ácido Poliglutâmico/análogos & derivados , Células-Tronco/metabolismo , Engenharia Tecidual/métodos , Animais , Cartilagem/metabolismo , Diferenciação Celular , Linhagem Celular , Condrócitos/metabolismo , Camundongos , Microscopia Eletrônica de Varredura/métodos , Ácido Poliglutâmico/química , Reologia/métodos , Termogravimetria/métodos , Tecidos Suporte/química
13.
Int J Biol Macromol ; 187: 91-104, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34298048

RESUMO

Developing a wound dressing for the treatment of large and irregular-shaped wounds remains a great challenge. Herein we developed novel printable bionic hydrogels with antibacterial and antioxidant properties which could effectively overcome the challenge by inhibiting inflammation and accelerating wound healing. The CMC/PL (CP) hydrogels were customized with glycidyl methacrylate (GMA) modified carboxymethyl cellulose (CMC) and ε-polylysine (ε-PL) via ultraviolet (UV) light polymerization using a 3D printer. Except for the high compression modulus (238 kPa), stable rheological properties, and effective degradability, these CP hydrogels also had an excellent inhibitory effect (95%) on both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Remarkably, CP hydrogels could remove the excessive reactive oxygen species (ROS) and protect the fibroblasts from damage. Compared with the commercial dressing (Tegaderm ™ film), CP hydrogels showed a better ability to increase the expression of VEGF and CD31, accelerate granulation tissue regeneration, and promote wound healing. This work provides a new strategy to fabricate on-demand multi-functional hydrogels in the field of skin tissue engineering.


Assuntos
Antibacterianos , Antioxidantes , Escherichia coli/crescimento & desenvolvimento , Hidrogéis , Impressão Tridimensional , Pele , Staphylococcus aureus/crescimento & desenvolvimento , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Celulose/química , Celulose/farmacologia , Hidrogéis/química , Hidrogéis/farmacologia , Camundongos , Células NIH 3T3 , Polilisina/química , Polilisina/farmacologia , Ratos , Ratos Sprague-Dawley , Pele/lesões , Pele/metabolismo
14.
J Hazard Mater ; 411: 125184, 2021 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-33858118

RESUMO

Trace detection of fluoride ion has gained increasing attention due to fluoride's close association with biological and environmental processes. Herein, we construct a novel hybrid nanosystem consisting of carbon dots and curcumin for sensitive and selective sensing of F-. Carbon dots are synthesized by hydrothermal treatment of 2,3-diaminopyridine and selenourea in hydrochloric acid. This material is employed as the fluorescent indicator that exhibits intense blue and yellow emission with quantum yields of 12% and 33%, respectively. Curcumin, possessing an absorption peak at 532 nm, can significantly quench the yellow fluorescence of carbon dots through inner filter effect. Curcumin is also used to specifically recognize F-. When F- is added, the curcumin-F- complex generates, which leads to the hypochromatic shift of the absorption band from 532 to 430 nm. In such a case, the inner filter effect reduces, and yellow fluorescence of carbon dots recovers. Thus, a fluorescence turn-on sensor of F- is built based on the carbon dots/curcumin system. The limits of detection and quantitation are measured to be 0.39 and 1.30 µM, respectively. For real usage, the proposed method is applied to determinate F- in tap water and milk samples with relative standard deviations below 7.9%.


Assuntos
Curcumina , Pontos Quânticos , Carbono , Fluorescência , Corantes Fluorescentes , Fluoretos , Espectrometria de Fluorescência
15.
Ann Plast Surg ; 86(3S Suppl 2): S189-S193, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33651013

RESUMO

OBJECTIVE: There has been an increase in use of autologous costal cartilage for nasal tip shaping material. However, there are deficiencies in using this plastic material, such as a hard nasal tip, poor nasal tip elasticity and mobility postoperatively, and some nasal tip skin thinning that allows the shape of the cartilage grafts to be felt on the surface of the nasal tip skin. To address these problems, we have combined autologous nasal septum cartilage and conchal cartilage as nasal tip graft materials, which achieve a natural shape and realistic touch postoperatively. METHODS: From January 2017 to September 2019, a total of 47 patients with nasal septal cartilage combined with conchal cartilage transplantation for nasal tip plasty were screened for a retrospective study. They were followed up and evaluated for 6 to 25 months postoperatively. The operation was performed through an incision in the nose. During the operation, a piece of septal cartilage was cut to make a columella strut. Using one side of the cavitas conchae and cymba conchae cartilage as the graft material, 2 long strips of cartilage were excised for the septum extension graft, and a piece of cap cartilage was transplanted to the top of the nasal tip cartilage scaffold. RESULTS: The time between the operation and follow-up was greater than 6 months, with the range of follow-up and evaluation being from 6 to 25 months. Forty-six patients were satisfied with the shape of the nose. One patient complained that the nasal tip was round and large after 1 month postoperatively, but after 6 months, he was satisfied with the shape of the nasal tip after daily use of a nasal clip. In one male case, the implant was removed more than 7 months postoperatively because of hematoma and infection secondary to nasal trauma. There were 2 cases of nasal tip numbness, both of which recovered spontaneously after 12 months. After the operation, the shape of the nasal tip was round, no cartilage contour was seen on the surface, no collapse of upper nasal tip, no obvious deviation of the nasal dorsum and columella, and no deformation and displacement of the nasal tip cartilage scaffold. At the 6-month follow-up visit, each patient palpated the nasal tip for evaluation. Most patients felt their nose felt realistic and that they had good nasal tip motion. Only 1 patient felt that the nasal tip and the nasal dorsum were moving together when the tip of the nose was being pushed. CONCLUSIONS: Nasal septum cartilage and conchal cartilage can be combined as a graft material for reconstruction of the nasal tip. This technique uses different characteristics of each of the 2 cartilages to achieve a more realistic and esthetic outcome.


Assuntos
Cartilagem Costal , Rinoplastia , Humanos , Masculino , Cartilagens Nasais/cirurgia , Septo Nasal/cirurgia , Nariz/cirurgia , Estudos Retrospectivos
16.
J Clin Apher ; 36(3): 454-464, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33598970

RESUMO

BACKGROUND: Ulcerative colitis (UC) is a refractory disease with unclear etiology. Studies have shown that UC is closely associated with gut microbiota dysbiosis. Adsorptive granulomonocytapheresis (GMA) using an Adacolumn has been found to treat UC effectively, but its underlying mechanism of treatment has not been fully elucidated. In this study, we aimed to investigate the influence of GMA on the gut microbiota in patients with active UC. METHODS: We conducted a single-center prospective analysis of patients with active UC who received GMA therapy and ultimately achieved clinical remission. Stool samples of healthy controls and patients before and after 5 or 10 sessions of GMA therapy were collected. Subsequently, high-throughput sequencing of the 16S rRNA V3 and V4 gene region of the stool was conducted and clustering of operational taxonomic units and species annotation were performed. RESULTS: Gut microbial profiles in patients with UC were characterized by low bacterial diversity. After 5 or 10 sessions of GMA therapy, the gut microbiota diversity in patients with UC increased and was similar to that of healthy controls. UC was further characterized by increased abundances of Proteobacteria and Bacteroides, as well as decreased abundances of Faecalibacterium, Roseburia, Firmicutes, and Dialister; however, after GMA therapy, the abundance of Bacteroides decreased, whereas those of Faecalibacterium, Roseburia, and Firmicutes increased. CONCLUSIONS: Active UC is associated with gut microbiota dysbiosis. GMA therapy exerts a strong regulatory effect on the gut microbiota in patients with UC.


Assuntos
Colite Ulcerativa/terapia , Microbioma Gastrointestinal , Leucaférese/métodos , Adulto , Colite Ulcerativa/microbiologia , Disbiose/microbiologia , Feminino , Granulócitos , Humanos , Masculino , Pessoa de Meia-Idade , Monócitos , Estudos Prospectivos
17.
Ann Plast Surg ; 86(3S Suppl 2): S265-S268, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33443877

RESUMO

ABSTRACT: Reconstruction of total facial deformities and defects has been a major challenge of reconstructive surgery. Allotransplantation is limited by the number of donors and the need for life-long immunosuppression. Autotransplantation, where multiple autogenous tissue grafts from various donor sites are used to repair facial defects, inevitably leaves conspicuous patchwork scars. A prefabricated monoblock flap, although the preferred treatment modality, is limited by insufficient blood supply and the large size of the flap. In the Journal of Craniofacial Surgery (2014;25:21-25), Li et al applied the technique of flap prefabrication and stem cell-assisted tissue expansion to reconstruct total facial injuries, but the operations were complicated and the final expanded flap area was also uncertain. This article introduces an approach to reconstruct total facial injuries with a prefabricated expanded thoracic flap combined with an expanded scalp flap (called combined flaps), which not only solves the limitations of blood supply and expanded volume but also reduces patchwork scars. The approach is a simple, feasible, and effective surgical method for total face resurfacing.


Assuntos
Queimaduras , Traumatismos Faciais , Procedimentos Cirúrgicos Reconstrutivos , Queimaduras/cirurgia , Cicatriz/cirurgia , Traumatismos Faciais/cirurgia , Humanos , Couro Cabeludo/cirurgia , Transplante de Pele , Retalhos Cirúrgicos , Expansão de Tecido
18.
Int J Biol Macromol ; 166: 940-947, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33152361

RESUMO

While ionic liquids (ILs) have been considered as effective and "green" solvents for biopolymer processing, regeneration of IL-dissolved biopolymers could largely impact biopolymer structure and properties. This study indicates that the reconstitution of chitosan structure during regeneration from 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) depends on anti-solvent (water, methanol or ethanol) largely. Irrespective of anti-solvent, the chitosan chemical structure was not varied by dissolution or regeneration. With water, the regenerated chitosan had the highest crystallinity index of 54.18%, followed by those with methanol (35.07%) and ethanol (25.65%). Water as an anti-solvent could promote chitosan chain rearrangement, leading to the formation of an ordered aggregated structure and crystallites. Density functional theory (DFT) simulation indicates that the number of hydrogen bonds formed between anti-solvents and [Emim][OAc] was in the order of water > methanol > ethanol. With water used for regeneration, the aggregation and rearrangement of chitosan chains occurred more easily.


Assuntos
Quitosana/análogos & derivados , Cristais Líquidos/química , Álcoois/química , Ligação de Hidrogênio , Imidazóis/química , Solventes/química , Água/química
19.
Int J Biol Macromol ; 167: 1508-1516, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33212107

RESUMO

For the problems in the research on differentiation of mesenchymal stem cells (BMSCs), such as poor differentiation tendency and low differentiation efficiency, a novel photo-crosslinked extracellular matrix (ECM) inspired double network hydrogel that composed of poly(γ-glutamic acid) (γ-PGA) hydrogel and Fe3+ ligand coordination was designed and manufactured. Compared with those traditional γ-PGA based hydrogels, the introduction of Fe3+ significantly enhanced the mechanical properties of the hydrogel and accelerated the chondrogenesis efficiency of BMSCs chondrogenesis. The experimental results confirmed that the mechanical properties of hydrogel enhanced by the introduction of metal ions Fe3+ could promote BMSCs proliferation, induce cartilage-specific gene expression, and increase secretion of hydroxyproline (HYP) and glycosaminoglycan (GAG). As a result, this method could promote chondrogenic differentiation of BMSCs, accelerate the regeneration of cartilage, and was prospective to be conducive to the research work of cartilage defect repair. Thus, the mechanically enhanced γ-PGA hydrogel scaffold by Fe3+ could mediate BMSCs differentiation and provide a scientific and theoretical basis for research and development of biomedical materials on cartilage tissue engineering field.


Assuntos
Biomimética/métodos , Cartilagem/efeitos dos fármacos , Condrogênese/efeitos dos fármacos , Hidrogéis/química , Ferro/química , Células-Tronco Mesenquimais/citologia , Ácido Poliglutâmico/análogos & derivados , Engenharia Tecidual/métodos , Materiais Biocompatíveis/química , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Matriz Extracelular/metabolismo , Glicosaminoglicanos/metabolismo , Hidrogéis/síntese química , Hidrogéis/efeitos da radiação , Hidroxiprolina/metabolismo , Ligantes , Células-Tronco Mesenquimais/efeitos dos fármacos , Metacrilatos/química , Fotoquímica , Ácido Poliglutâmico/química , Reologia , Tecidos Suporte/química
20.
J Interv Cardiol ; 2020: 1031675, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33192184

RESUMO

AIM: Based on optical coherence tomography (OCT), we aimed to determine the diagnosis, clinical characteristics, and interventions of braid-like coronary arteries, which are rare and tend to be diagnosed as a woven coronary artery (WCA) anomaly. METHODS AND RESULTS: We identified braid-like lesions on coronary angiography (CAG) in 7 patients (6 men; median age 47 years; age range 26 to 57 years). All patients were heavy smokers. Four patients were diagnosed with an old myocardial infarction and the other 3 with unstable angina. The braid-like lesions were located in the left anterior descending arteries in 2 patients and in the right coronary arteries in the other 5. TIMI grade 2 flow was observed in all involved vessels. OCT findings of all lesions were consistent with recanalization of organized thrombi, which consisted of septa that divided the lumen into multiple small cavities communicating with each other. No separate three-layered structure could be defined. Based on the significance of the stenosis and its related symptoms, drug-eluting stents were implanted in all of the lesions. All patients experienced symptomatic improvement after the intervention and were followed up event-free for 12 months. CONCLUSIONS: Braid-like coronary arteries are likely to undergo recanalization of organized thrombi rather than WCA according to our OCT findings. The majority of cases affect men who smoke heavily. Percutaneous stent implantation may be beneficial in selected patients when feasible.


Assuntos
Angiografia Coronária/métodos , Anomalias dos Vasos Coronários , Vasos Coronários , Intervenção Coronária Percutânea , Tomografia de Coerência Óptica/métodos , Anomalias dos Vasos Coronários/diagnóstico , Anomalias dos Vasos Coronários/cirurgia , Vasos Coronários/diagnóstico por imagem , Vasos Coronários/patologia , Diagnóstico Diferencial , Stents Farmacológicos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Isquemia Miocárdica/diagnóstico , Isquemia Miocárdica/psicologia , Isquemia Miocárdica/cirurgia , Intervenção Coronária Percutânea/instrumentação , Intervenção Coronária Percutânea/métodos , Fatores de Risco , Fumar/epidemiologia , Resultado do Tratamento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...