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1.
Plant Cell ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069643

RESUMO

Abscisic acid (ABA)-activated inward Ca2+-permeable channels in the plasma membrane (PM) of guard cells are required for the initiation and regulation of ABA-specific cytosolic Ca2+ signaling and stomatal closure in plants. But the identities of the PM Ca2+ channels are still unknown. We hypothesized that the ABA-activated Ca2+ channels consist of multiple CYCLIC NUCLEOTIDE GATED CHANNEL (CNGC) proteins from the CNGC family, which is known as a Ca2+-permeable channel family in Arabidopsis (Arabidopsis thaliana). In this research, we observed high expression of multiple CNGC genes in Arabidopsis guard cells, namely CNGC5, CNGC6, CNGC9 and CNGC12. The T-DNA insertional loss-of-function quadruple mutant cngc5-1 cngc6-2 cngc9-1 cngc12-1 (hereafter c5/6/9/12) showed a strong ABA-insensitive phenotype of stomatal closure. Further analysis revealed that ABA-activated Ca2+ channel currents were impaired, and ABA-specific cytosolic Ca2+ oscillation patterns were disrupted in c5/6/9/12 guard cells compared with in wild-type guard cells. All ABA-related phenotypes of the c5/6/9/12 mutant were successfully rescued by the expression of a single gene out of the four CNGCs under the respective native promoter. Thus, our findings reveal a type of ABA-activated PM Ca2+ channel comprised of multiple CNGCs, which is essential for ABA-specific Ca2+ signaling of guard cells and ABA-induced stomatal closure in Arabidopsis.

2.
Front Neurosci ; 16: 938200, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36090261

RESUMO

Objective: To investigate the analgesic mechanism of electroacupuncture (EA) in rats with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods: Thirty male SD rats were randomly divided into sham group, model group and EA group, with ten rats in each group. The CP/CPPS model was prepared by injecting 50 µL of complete Freund's adjuvant (CFA) into the ventral lobes of the prostate tissue, and the sham group was injected with the same dose of saline. After 14 days of modeling, EA was applied to Guanyuan (CV4), Zhongji (CV3), Sanyinjiao (SP6) and Huiyang (BL35) in the EA group. After four courses, H&E staining was performed to observe the prostate tissue morphology, transcriptome sequencing (RNA-Seq) was performed for each group, and the selected signaling pathways were verified by qRT-PCR. Results: The RNA-Seq analysis results suggested that the analgesic effect of EA on CP/CPPS may be achieved by regulating prostate gene expression, which may be related to multiple biological processes and signaling pathways. qRT-PCR results showed that the vanillic acid receptor subtype 1 of the transient receptor potential (TRPV1), phospholipase C (PLC), protein kinase C (PKC), cyclic adenosine monophosphate (cAMP), and protein kinase A (PKA) were all upregulated in the model group compared to the sham group (p < 0.01). Compared with the model group, TRPV1, PLC, PKC, cAMP, and PKA were all downregulated in the EA group (p < 0.05, p < 0.01). Conclusion: The analgesic mechanism of EA on CP/CPPS may be achieved through modulation of cAMP-PKA-TRPV1/PLC-PKC-TRPV1 signaling pathway.

3.
Front Plant Sci ; 13: 988211, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119600

RESUMO

Excessive nitrogen (N) fertilizer has threatened the survivability and sustainability of agriculture. Improving N productivity is promising to address the above issue. Therefore, the field experiment, which investigated the effect of no-till and N fertilizer reduction on water use and N productivity of spring wheat (Triticum aestivum L.), was conducted at Wuwei experimental station in northwestern China. There were two tillage practices (conventional tillage, CT; and no-till with previous plastic film mulching, NT) and three N fertilizer rates (135 kg N ha-1, N1; 180 kg N ha-1, N2; and 225 kg N ha-1, N3). The results showed that NT lowered soil evaporation (SE) by 22.4% while increasing the ratio of transpiration to evapotranspiration (T/ET) by 13.6%, compared with CT. In addition, NT improved the total N accumulation by 11.5% and enhanced N translocation (NT) quantity, rate, and contribution by a range of 6.2-23.3%. Ultimately, NT increased grain yield (GY), N partial factor productivity, and N harvest index by 13.4, 13.1, and 26.0%, respectively. Overall, N1 increased SE (13.6%) but decreased T/ET (6.1%) compared with N3. While, N2 enhanced NT quantity, rate, and contribution by a range of 6.0-15.2%. With the integration of NT, N2 achieved the same level of GY and N harvest index as N3 and promoted N partial factor productivity by 11.7%. The significant positive correlation of NT relative to T/ET and GY indicated that improving T/ET was essential for achieving higher NT. Therefore, we concluded that no-till coupled with N fertilizer rate at 180 kg N ha-1 was a preferable management option to boost the N productivity of spring wheat in arid areas.

4.
Chem Biodivers ; : e202200485, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069208

RESUMO

Curcumin is a potential plant-derived drug for the treatment of breast cancer. Poor solubility and bioavailability are the main factors that limit its clinical application. Various structural modification strategies have been developed to improve the anti-breast cancer activity of curcumin. This review focuses on the difference of modification sites and heterocyclic/non-heterocyclic modifications to systematically summarize curcumin derivatives with better anti-breast cancer activity.

5.
Eur J Cancer ; 175: 125-135, 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36113242

RESUMO

BACKGROUND: A phase 1a first-in-human study evaluated the safety/tolerability, preliminary antitumour activity and pharmacokinetics of the oral MEK1/2 inhibitor FCN-159 in Chinese patients with advanced, NRAS-mutant melanoma. PATIENTS AND METHODS: Patients received a single FCN-159 dose at assigned levels, proceeding to continuous dosing (once daily [QD] for 28-day cycles) if no dose-limiting toxicities (DLTs) occurred within the next 3 days. Dose escalation was initiated after review of data for the previous dose level. The primary end-point was incidence of DLTs after the first dose. RESULTS: Thirty-three patients were enrolled across nine FCN-159 dose groups (0.2-15 mg QD). One DLT occurred: grade 3 folliculitis in the 15-mg group. There was one grade >3 treatment-emergent adverse event (TEAE), death of unknown aetiology (not FCN-159 related). The most common FCN-159-related TEAE was rash (36.4%), and the incidence of grade ≥3 FCN-159-related TEAEs was 15.2%. Antitumour activity at QD doses <6 mg was limited; therefore, efficacy data are presented only for doses ≥6 mg (n = 21). The objective response and clinical benefit rates were 19.0% (four partial responses) and 52.4%, respectively. Median (95% confidence interval) duration of response and progression-free survival were 4.8 months (2.8-not reached) and 3.8 months (1.8-5.6), respectively. FCN-159 exposure increased dose-proportionately; geometric mean terminal half-life was 29.9-56.9 h. CONCLUSIONS: FCN-159 was well tolerated and demonstrated promising antitumour activity at doses ≥6 mg QD in patients with advanced, NRAS-mutant melanoma. The recommended phase 2 dose was 12 mg QD. GOV IDENTIFIER: NCT03932253. https://clinicaltrials.gov/ct2/show/NCT03932253.

6.
BMJ Open ; 12(9): e060912, 2022 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-36104141

RESUMO

OBJECTIVE: This study systematically reviewed the effect of melatonin (MLT) on quality of life (QoL) and symptoms among patients with cancer. DESIGN: Systematic review and meta-analysis. DATA SOURCES: Cochrane Library, PubMed, Embase, Web of Science, Medline, CINAHL, Scopus, ClinicalTrials.gov, China Biology Medicine (CBM), ProQuest and Open Grey were searched from inception to November 2021. ELIGIBILITY CRITERIA: We included randomised controlled trials (RCTs) assessing the effects of MLT on QoL, sleep quality, fatigue, depression, pain, stomatitis rate and stomatitis severity in adult patients with cancer, without language restrictions. Studies that reported the effects of MLT along with other interventions and had incomplete or absent outcome data were excluded. DATA EXTRACTION AND SYNTHESIS: Two independent reviewers extracted data, and another two reviewers assessed the risk of bias. The risk of bias for each eligible study was assessed using the Cochrane assessment tool. The mean difference or standard mean difference (SMD) with 95% CIs was used in the computation of continuous variables to synthesise data. The relative risk was used for dichotomous outcomes. Heterogeneity was assessed and quantified (I2 statistic). RESULTS: A total of 19 qualified studies that included 2101 patients with cancer (MLT: 1078, control: 1023) were included in the meta-analysis. The results indicated that MLT had no significant effect on QoL (SMD=-0.01, 95% CI (-0.14 to 0.11), p=0.83), sleep quality (SMD=-0.18, 95% CI (-0.62 to 0.26), p=0.42), fatigue (SMD=-0.34, 95% CI (-0.73 to 0.06), p=0.10), pain (SMD=-0.34, 95% CI (-0.7 to 0.02), p=0.06) or stomatitis severity (RR=0.78, 95% CI (0.47 to 1.30), p=0.35). MLT reduced stomatitis rate among patients with cancer (RR=0.47, 95% CI (0.26 to 0.88), p=0.02), except those with head and neck cancer (RR=1.09, 95% CI (0.92 to 1.29), p=0.35). MLT eased depression in patients who received administration for more than 14 days (SMD=-0.14, 95% CI (-0.27 to -0.01), p=0.03) and those who underwent surgery (SMD=-0.17, 95% CI (-0.32 to -0.03), p=0.02). CONCLUSION: The findings showed that MLT did not improve the QoL, sleep quality, fatigue, pain or stomatitis severity among patients with cancer. It had a limited effect on decreasing the stomatitis rate and easing depression. Different treatments, durations and cancer types were the main sources of heterogeneity. Further large-scale RCTs are urgently needed. In addition, the effects of different combinations of MLT dosage and duration, administration types and joint measures are worthy of further study. PROSPERO REGISTRATION NUMBER: CRD42021292855.


Assuntos
Melatonina , Neoplasias , Estomatite , Adulto , Fadiga/etiologia , Humanos , Melatonina/uso terapêutico , Neoplasias/complicações , Neoplasias/tratamento farmacológico , Dor , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto
7.
Artigo em Inglês | MEDLINE | ID: mdl-35941500

RESUMO

Although increased attempts to preserve biodiversity ecosystems have been widely publicized, bibliometric research of biodiversity loss remains limited. Using VOSviewer, we hope to provide a bibliometric assessment of global research trends on biodiversity loss from 1990 to 2021. Document type, language, publication trend, countries, institutions, Author Keywords, and Keywords Plus were all examined. This study recorded a total of 6599 publications from the Web of Science Core Collection database. According to the findings, biodiversity loss research is expected to rise dramatically in the near future. However, the role of social sciences and economics in biodiversity loss studies has received little attention. The USA made the most significant contribution in this field. Biological Conservation was the most productive journal, and Proceedings of the National Academy of Sciences of the United States of America was the most influential journal in biodiversity loss literature. Eisenhauer, N was the most prolific author, and Collen, B was the most referenced. Biodiversity, biodiversity loss mechanisms, biodiversity loss drivers, conservation, and climate change have been the topic of previous research. Possible future research hotspots may include species diversity and many elements of biodiversity. Lastly, the outcomes of this study suggest that existing socio-economic concerns can be integrated into decision-making processes to improve biodiversity conservation.

8.
Exp Cell Res ; 419(2): 113324, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-36002046

RESUMO

Osteogenic differentiation of periodontal ligament stem cells (PDLSCs) is limited in hypoxia, and HIF-1α is key to the response to hypoxia. However, its mechanisms remain largely unknown. This study discovered an osteogenesis-related gene sensitive to hypoxia in PDLSCs, and investigated the molecular mechanisms between HIF-1α and the gene. NOG, a gene that negatively regulates osteogenesis, was discovered by RNA-seq. Under normoxic conditions, HIF-1α overexpression led to enhanced expression of NOG/Noggin and inhibited the expression of osteogenesis-related genes, while inhibition of HIF-1α reversed this effect. The expression of HIF-1α, NOG/Noggin and the osteogenesis-related genes were detected by qRT-PCR or Western blot. Mechanistically, we verified that HIF-1α binds to the hypoxia response element (-1505 to -1502) in the promotor of NOG to enhance secretion of Noggin by chromatin immunoprecipitation and a dual-luciferase reporter assay. IHC staining findings in an animal model verified that Noggin-associated osteogenic differentiation was inhibited in hypoxia. NOG displayed a concordant relationship with HIF-1α, and secreted more with increasing of HIF-1α. Hypoxia stabilized HIF-1α, which bound to the HRE (-1505 to -1502) of the NOG promotor to enhance NOG transcription resulted in inhibiting osteogenic differentiation of PDLSCs. This study offers a promising therapy for periodontitis.


Assuntos
Osteogênese , Ligamento Periodontal , Animais , Diferenciação Celular/genética , Células Cultivadas , Hipóxia/metabolismo , Osteogênese/genética , Ligamento Periodontal/metabolismo , Células-Tronco
9.
Chem Commun (Camb) ; 58(68): 9532-9535, 2022 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-35924915

RESUMO

A series of site-diversified, fully functionalized diazirine probes are constructed based on a scaffold shared by several marketed EGFR-targeted drugs. The integrated analysis of protein targets of the site-diversified probe toolkit not only unveils more complete target space and helps suggest false positive targets, but also reveals dynamic events of multi-domain target-ligand interaction.


Assuntos
Diazometano , Proteômica , Diazometano/química , Ligantes , Proteínas
10.
PLoS One ; 17(8): e0271075, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35944028

RESUMO

Tetracycline (TC) is widely-used antibiotic pollutant with high toxicity, refractory, persistence and bacteriostasis, and its removal from water needs to be enhanced. In this work, a novel Graphite-UiO-66(Zr)/Ti electrode was successfully prepared and evaluated for electrochemical oxidation degradation of TC. The electrochemical performance tests indicate the Graphite-UiO-66(Zr)/Ti electrode had higher electrochemical oxidation activity, which achieved higher TC removal efficiency (98.1% ± 1.5%) than Ti plate (65.2% ± 3.5%), Graphite-MIL-53(Al)/Ti electrode (79.5% ± 2.9%) and Graphite-MIL-100(Fe)/Ti electrode (89.0% ± 2.6%). The influence of operating condition was also systematically studied, and the optimized condition was pH 5.0, 20 mA/cm2 current density and 0.1 M electrolyte (Na2SO4). Through the liquid chromatography mass spectrometry (LC-MS), the TC degradation pathway by Graphite-UiO-66(Zr)/Ti electrode oxidation was proposed. Under the •OH free radical oxidative decomposition effect, the double bond, phenolic group and amine group of TC were attacked. TC was transformed into intermediate product ① (m/z = 447), then was further degraded to intermediates ② (m/z = 401) and ③ (m/z = 417). The latter was fragmented into small fractions ④ (m/z = 194), ⑤but-2-enedioic acid (m/z = 116) and ⑥oxalic acid (m/z = 90, the proposed intermediate). In addition, TC removal remained at 89.6% ± 2.7% in the sixth cycle of operation, which confirmed the efficient reusability and stability for antibiotics removal from water.


Assuntos
Grafite , Poluentes Químicos da Água , Antibacterianos/análise , Eletrodos , Grafite/química , Estruturas Metalorgânicas , Oxirredução , Ácidos Ftálicos , Tetraciclina/análise , Titânio/química , Água , Poluentes Químicos da Água/análise
11.
Cancers (Basel) ; 14(15)2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35954435

RESUMO

As the most common neuro-epithelial tumors of the central nervous system in adults, gliomas are highly malignant and easy to recurrence, with a dismal prognosis. Imaging studies are indispensable for tracking tumor progression (TP) or treatment-related changes (TRCs). During follow-up, distinguishing TRCs from TP in treated patients with gliomas remains challenging as both share similar clinical symptoms and morphological imaging characteristics (with new and/or increasing enhancing mass lesions) and fulfill criteria for progression. Thus, the early identification of TP and TRCs is of great significance for determining the prognosis and treatment. Histopathological biopsy is currently the gold standard for TP and TRC diagnosis. However, the invasive nature of this technique limits its clinical application. Advanced imaging methods (e.g., diffusion magnetic resonance imaging (MRI), perfusion MRI, magnetic resonance spectroscopy (MRS), positron emission tomography (PET), amide proton transfer (APT) and artificial intelligence (AI)) provide a non-invasive and feasible technical means for identifying of TP and TRCs at an early stage, which have recently become research hotspots. This paper reviews the current research on using the abovementioned advanced imaging methods to identify TP and TRCs of gliomas. First, the review focuses on the pathological changes of the two entities to establish a theoretical basis for imaging identification. Then, it elaborates on the application of different imaging techniques and AI in identifying the two entities. Finally, the current challenges and future prospects of these techniques and methods are discussed.

12.
Front Cell Infect Microbiol ; 12: 949514, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35937700

RESUMO

Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection (STI) and remains a major public health challenge, especially in less-developed regions. Establishing a rapid, inexpensive, and easy-to-interpret point-of-care (POC) testing system for C. trachomatis could be critical for its treatment and limiting further transmission. Here, we devised a novel approach termed a multiple cross displacement amplification integrated with gold nanoparticle-based lateral flow biosensor (MCDA-AuNPs-LFB) for the highly specific, sensitive, user-friendly, and rapid identification of C. trachomatis in clinical samples. A suite of MCDA primers based on the C. trachomatis ompA gene from 14 serological variants (serovar A-K, L1, L2, and L3) were successfully designed and used to establish the assay. Optimal assay conditions were identified at 67°C, and the detection procedure, including nucleic acid preparation (approximately 5 min), MCDA amplification (30 min), and AuNPs-LFB visual readout (within 2 min), was completed within 40 min. The all-in cost for each test was approximately $5.5 USD. The limit of detection (LoD) was 10 copies/reaction, and no cross-reaction was observed with non-C. trachomatis microbes. A total of 135 suspected C. trachomatis-infection genital secretion samples were collected and simultaneously detected using real-time quantitative PCR (qPCR) in our assay. Compared with the qPCR technology, the MCDA-AuNPs-LFB sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 96.20%, 94.92%, and 100%, respectively. Hence, our MCDA-AuNP-LFB assay exhibited considerable potential for POC testing and could be used to identify C. trachomatis in clinical settings, particularly in low-income regions.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Técnicas Biossensoriais/métodos , Chlamydia trachomatis/genética , Ouro , Técnicas de Amplificação de Ácido Nucleico/métodos , Sistemas Automatizados de Assistência Junto ao Leito , Sensibilidade e Especificidade , Temperatura
13.
Acta Histochem ; 124(7): 151938, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35981451

RESUMO

RILP (Rab-interacting lysosomal protein) is a key regulator of lysosomal transport and a potential tumor suppressor. However, the role of RILP in prostate cancer and the underlying mechanism of RILP in regulating the proliferation, migration, and invasion of prostate cancer cells remain to be studied. In this study, we confirmed RalGDS (Ral guanine nucleotide dissociation stimulator) as the interaction partner of RILP in PC3 prostate cancer cells. Immunofluorescence microscopy showed that RILP recruits RalGDS to the lysosomal compartment. We found that RILP inhibits the activation of RalA and downstream effector RalBP1, and negatively regulates the downstream molecular phosphorylation of Ras. We showed that RILP inhibits the proliferation, migration, and invasion of PC3 prostate cancer cells, which may give rise to novel ideas for cancer treatment.

14.
Tohoku J Exp Med ; 258(2): 149-157, 2022 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-36002253

RESUMO

Liver cancer is one of the most common cancer forms and a significant contributor to global cancer-associated mortality. Hepatitis B virus (HBV) infection contributes enormously to HCC development and progression. Despite this, the molecular basis of liver tumorigenesis is not clear. This work focused on identifying the relevant genetic markers and available drugs for treating HBV-related HCC. Differentially expressed genes (DEGs) from HBV-related HCC samples and corresponding healthy samples were identified from GSE62232 and GSE121248 datasets from the GEO2R repository (Gene Expression Omnibus). The Venn diagram software screened the overlapping DEGs between these two datasets. The DEGs were functionally assessed using protein-protein interaction (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses by different bioinformatic methods, and hub genes were screened. Hub genes and related drugs were verified by the GEPIA2 (Gene Expression Profiling Interactive Analysis) web server and the Quartata Web online platform. Overall, 116 DEGs (88 up-regulated and 28 down-regulated) related to signal transduction and metabolic pathways were identified. The nine significant target hub genes were TOP2A, RRM2, DTL, ECT2, ASPM, ANLN, BUB1B, CCNB1, and CDK1. Moreover, one screened drug, Fostamatinib, was targeted to CDK1. Our study identified three genes and associated drugs as probable targets for studying HBV-related HCC.


Assuntos
Carcinoma Hepatocelular , Hepatite B , Neoplasias Hepáticas , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/genética , Biologia Computacional/métodos , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Marcadores Genéticos , Hepatite B/complicações , Hepatite B/tratamento farmacológico , Hepatite B/genética , Vírus da Hepatite B/genética , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/genética , Proteínas do Tecido Nervoso/genética
15.
J Hazard Mater ; 440: 129741, 2022 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-35985217

RESUMO

Biochar currently served as the support for dispersed metal nanoparticles and cooperated with pyrite to generate more reactive radicals in organic pollution degradation system. But the mechanism of interaction between biochar and pyrite has not been elucidated. In this paper, biochar with oxygen-containing functional groups (OFGs) served as a stable dispersant to prepare nano-FexSy loaded biochar materials (BCOFGs@nano-FexSy). BCOFGs coordinated with nano-FexSy to overcome its drawbacks, boosting QNC removal efficiency from 28.64% to 100%. The XPS and the linear sweep voltammetry (LSV) results revealed higher Fe(II) content and higher electron transfer rate on used BCOFGs@nano-FexSy, further validating that hydroxyl functional groups on biochar surface provided electrons to Fe(III) to achieve efficient Fe(II)/Fe(III) cycling. Based on comparative experiments and studies on the roles of iron, S(II) species and OFGs, we clearly revealed that OFGs on biochar materials surface coordinated with nano-FexSy to catalyze the degradation of QNC. The degradation efficiency of BCOFGs@nano-FexSy for QNC was still as high as 91.39% after five cycles, providing full demonstrations that OFGs and S(II) as the abundant electron donor coordinated with Fe species for QNC catalytic degradation and further enhanced the catalytic performance and stability of nano-FexSy.


Assuntos
Compostos Férricos , Poluentes Químicos da Água , Carvão Vegetal , Compostos Ferrosos , Ferro , Oxigênio , Sulfetos , Poluentes Químicos da Água/análise
16.
Chemosphere ; 307(Pt 3): 135843, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35970212

RESUMO

In this paper, a three-dimensional mathematical model of the rotary diesel particulate filter (RDPF) for truck diesel engine is established according to the fluid mechanics and porous media theory. The effects of inlet velocity and structure parameters (diameter ratio, expansion angle and filter length) on the flow uniformity in the RDPF are investigated. Furthermore, the Fuzzy grey relational analysis (FGRA) is employed to make a weight analysis of the influences of structure parameters on the regeneration performance and pressure drop of the RDPF. The results show that the velocity uniformity in the RDPF can be improved by properly reducing the inlet velocity, diameter ratio or expansion angle θ1. The capture-regeneration volume ratio with 8-10 is appropriate range for the structural optimization. Finally, the expansion angle θ1 is the most important structure parameter for the filter regeneration performance (regeneration time R = 0.8467; regeneration efficiency R = 0.6849) and the diameter ratio is the most important structure parameter for the pressure drop at the capture-regeneration "balance point" (R = 0.9352).


Assuntos
Material Particulado , Emissões de Veículos , Baías , Poeira , Veículos Automotores , Material Particulado/análise , Emissões de Veículos/análise
17.
Front Pharmacol ; 13: 905547, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35784704

RESUMO

Aims: To evaluate the utility of fasudil in a rat model of contrast-associated acute kidney injury (CA-AKI) and explore its underlying mechanism through multiparametric renal magnetic resonance imaging (mpMRI). Methods: Experimental rats (n = 72) were grouped as follows: controls (n = 24), CA-AKI (n = 24), or CA-AKI + Fasudil (n = 24). All animals underwent two mpMRI studies (arterial spin labeling, T1 and T2 mapping) at baseline and post iopromide/fasudil injection (Days 1, 3, 7, and 13 respectively). Relative change in renal blood flow (ΔRBF), T1 (ΔT1) and T2 (ΔT2) values were assessed at specified time points. Serum levels of cystatin C (CysC) and interleukin-1ß (IL-1ß), and urinary neutrophil gelatinase-associated lipocalin (NGAL) concentrations were tested as laboratory biomarkers, in addition to examining renal histology and expression levels of various proteins (Rho-kinase [ROCK], α-smooth muscle actin [α-SMA]), hypoxia-inducible factor-1α (HIF-1α), and transforming growth factor-ß1 (TGF-ß1) that regulate renal fibrosis and hypoxia. Results: Compared with the control group, serum levels of CysC and IL-1ß, and urinary NGAL concentrations were clearly increased from Day 1 to Day 13 in the CA-AKI group (all p < 0.05). There were significant reductions in ΔT2 values on Days 1 and 3, and ΔT1 reductions were significantly more pronounced at all time points (Days 1-13) in the CA-AKI + Fasudil group (vs. CA-AKI) (all p < 0.05). Fasudil treatment lowered expression levels of ROCK-1, and p-MYPT1/MYPT1 proteins induced by iopromide, decreasing TGF-ß1 expression and suppressing both extracellular matrix accumulation and α-SMA expression relative to untreated status (all p < 0.05). Fasudil also enhanced PHD2 transcription and inhibition of HIF-1α expression after CA-AKI. Conclusions: In the context of CA-AKI, fasudil appears to reduce renal hypoxia, fibrosis, and dysfunction by activating (Rho/ROCK) or inhibiting (TGF-ß1, HIF-1α) certain signaling pathways and reducing α-SMA expression. Multiparametric MRI may be a viable noninvasive tool for monitoring CA-AKI pathophysiology during fasudil therapy.

18.
Front Neurol ; 13: 893401, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35812109

RESUMO

Background: Abdominal obesity and adipocytokines are closely related to atherosclerosis, and adiponectin level is considered one of the important clinical indicators. This study aimed to analyze the associations of abdominal visceral fat content and adiponectin level with intracranial atherosclerotic stenosis (ICAS). Methods: A total of 186 patients were enrolled in this study. Patients were distributed into ICAS and non-ICAS by the degree of artery stenosis. Plasma adiponectin levels and the ratio of visceral adipose tissue (VAT) to subcutaneous adipose tissue (SAT) were measured. The related factors of intracranial atherosclerotic stenosis were determined using multivariable logistic regression analysis. Results: The VAT/SAT ratio (OR, 26.08; 95% CI, 5.92-114.83; p < 0.001) and adiponectin (OR, 0.61; 95% CI, 0.44-0.84; p = 0.002) were found to be the independent predictors of ICAS in a multivariable logistic regression analysis. The prevalence of ICAS increased (T1: 27.4%; T2: 50.0%; T3: 75.8%) as the VAT/SAT ratio tertile increased (p < 0.001). The prevalence of ICAS decreased (T1: 72.6%; T2: 54.8%; T3: 25.8%) as the adiponectin tertile increased (p < 0.001). In ROC curves analysis, VAT/SAT ratio had a sensible accuracy for the prediction of ICAS. The optimal cut-off value of VAT/SAT ratio to predict ICAS in this study was 1.04 (AUC: 0.747; p < 0.001; sensitivity: 67.4%; specificity: 74.7%). The optimal adiponectin cutoff was 3.03 ug/ml (AUC: 0.716; p < 0.001; sensitivity:75.8%; specificity: 61.5%). Conclusion: Higher VAT/SAT ratio and lower plasma adiponectin levels were closely related to the increased risk of intracranial atherosclerotic stenosis.

19.
Front Microbiol ; 13: 914620, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35903464

RESUMO

Chlamydial infection, caused by Chlamydia trachomatis, is the most common bacterial sexually transmitted infection and remains a major public health problem worldwide, particularly in underdeveloped regions. Developing a rapid and sensitive point-of-care (POC) testing for accurate screening of C. trachomatis infection is critical for earlier treatment to prevent transmission. In this study, a novel diagnostic assay, loop-mediated isothermal amplification integrated with gold nanoparticle-based lateral flow biosensor (LAMP-LFB), was devised and applied for diagnosis of C. trachomatis in clinical samples. A set of LAMP primers based on the ompA gene from 14 C. trachomatis serological variants (serovar A-K, L1, L2, L3) was successfully designed and used for the development of C. trachomatis-LAMP-LFB assay. The optimal reaction system can be performed at a constant temperature of 67°C for 35 min. The total assay process, including genomic DNA extraction (~15 min), LAMP reaction (35 min), and LFB readout (~2 min), could be finished within 60 min. The C. trachomatis-LAMP-LFB could detect down to 50 copies/ml, and the specificity was 100%, no cross-reactions with other pathogens were observed. Hence, our C. trachomatis-LAMP-LFB was a rapid, reliable, sensitive, cost-effective, and easy-to-operate assay, which could offer an attractive POC testing tool for chlamydial infection screening, especially in resource starvation settings.

20.
ACS Appl Mater Interfaces ; 14(26): 29599-29612, 2022 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-35737456

RESUMO

Aggregation-induced emission (AIE)-active luminogens (AIEgens) have demonstrated exciting potential for the application in cancer phototheranostics. However, simultaneously achieving tumor-activated bright emission, enhanced reactive oxygen species (ROS) generation, high tumor accumulation, and minimized ROS depletion remains challenging. Here, a metal-organic framework (MOF) hybrid AIEgen theranostic platform is designed, termed A-NUiO@DCDA@ZIF-Cu, composed of an AIEgen-loaded hydrophobic UiO-66 (A-NUiO@DCDA) core and a Cu-doped hydrophilic ZIF-8 (ZIF-Cu) shell. The fluorescence emission and therapeutic ROS activity of AIEgens are restrained during delivery. After uptake by tumor tissues, ZIF-Cu decomposition occurs in response to an acidic tumor microenvironment (TME), and the hydrophobic A-NUiO@DCDA cores self-assemble into large particles, extremely increasing the tumor accumulation of AIEgens. This results in enhanced fluorescence imaging (FLI) and highly improved 1O2 generation ability during photodynamic therapy (PDT). Meanwhile, the released Cu2+ reacts to glutathione (GSH) to generate Cu+, which provides an extra chemodynamic therapy (CDT) function through Fenton-like reactions with overexpressed H2O2, resulting in the GSH depletion-enhanced ROS therapy. As a result of these characteristics, the MOF hybrid AIEgens can selectively kill tumors with excellent efficacy.


Assuntos
Estruturas Metalorgânicas , Neoplasias , Fotoquimioterapia , Linhagem Celular Tumoral , Glutationa , Humanos , Peróxido de Hidrogênio , Estruturas Metalorgânicas/farmacologia , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/química , Ácidos Ftálicos , Espécies Reativas de Oxigênio , Microambiente Tumoral
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